GREMLIN Database
CUSB - Cation efflux system protein CusB
UniProt: P77239 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14235
Length: 407 (311)
Sequences: 11789 (9022)
Seq/√Len: 511.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
345_D351_V3.4001.00
134_K152_D3.1071.00
149_P209_T3.0521.00
356_A369_S2.9941.00
98_T376_K2.7891.00
353_K375_E2.6591.00
145_Q215_D2.5541.00
340_R352_P2.4521.00
343_T353_K2.4281.00
162_Q202_Q2.4051.00
281_W299_E2.2681.00
102_G364_V2.2261.00
159_V162_Q2.1941.00
263_Q277_T2.1941.00
114_V246_V2.1881.00
101_R373_E2.1821.00
278_I298_L2.1501.00
115_S247_T2.1501.00
152_D209_T2.1361.00
349_R390_N2.1021.00
158_W198_L2.0811.00
145_Q148_T2.0731.00
265_T277_T2.0711.00
149_P211_K2.0251.00
329_S365_T2.0141.00
326_L364_V2.0001.00
136_Y149_P1.9931.00
136_Y152_D1.9711.00
248_A315_L1.9521.00
344_V391_I1.9081.00
338_E354_R1.9021.00
101_R323_P1.8911.00
169_R196_R1.8771.00
143_K217_V1.8691.00
372_A375_E1.8671.00
193_A196_R1.8641.00
212_A238_I1.8611.00
93_G392_S1.8521.00
351_V387_S1.8231.00
167_L171_T1.8191.00
274_K306_A1.8111.00
107_A316_Q1.8101.00
114_V244_V1.7951.00
146_K215_D1.7931.00
356_A368_R1.7851.00
120_Q240_G1.7741.00
358_F368_R1.7551.00
185_L189_G1.7401.00
182_L186_R1.7381.00
175_A179_E1.7101.00
266_L300_V1.7091.00
102_G326_L1.7021.00
127_R207_R1.6901.00
174_T178_T1.6871.00
358_F366_A1.6751.00
96_T393_G1.6741.00
264_F298_L1.6731.00
186_R189_G1.6621.00
355_V367_L1.6611.00
218_I236_A1.6591.00
359_Q366_A1.6561.00
162_Q166_L1.6411.00
166_L169_R1.6391.00
99_V325_L1.6381.00
248_A298_L1.6211.00
165_Y192_E1.6111.00
109_S316_Q1.6101.00
350_F378_V1.6051.00
132_I223_L1.5951.00
341_V355_V1.5731.00
263_Q279_R1.5481.00
268_V313_A1.5461.00
95_K380_S1.5431.00
96_T344_V1.5301.00
265_T275_T1.5281.00
357_V365_T1.5271.00
245_W297_R1.5261.00
196_R199_I1.4921.00
99_V374_G1.4791.00
178_T182_L1.4771.00
199_I202_Q1.4731.00
329_S363_G1.4631.00
343_T375_E1.4361.00
109_S314_W1.4361.00
327_I377_V1.4351.00
96_T376_K1.4321.00
189_G193_A1.4061.00
247_T295_Q1.3951.00
132_I151_L1.3921.00
334_D339_Q1.3851.00
165_Y169_R1.3821.00
200_A204_I1.3691.00
249_A295_Q1.3641.00
251_P254_I1.3591.00
93_G394_A1.3541.00
129_A231_K1.3411.00
121_Y237_K1.3331.00
265_T318_N1.3091.00
243_P299_E1.2991.00
240_G243_P1.2991.00
219_T239_Q1.2901.00
267_T316_Q1.2861.00
246_V313_A1.2811.00
265_T316_Q1.2591.00
157_D160_E1.2591.00
103_P323_P1.2381.00
137_P148_T1.2271.00
130_G153_L1.2261.00
150_L236_A1.2231.00
164_E167_L1.2181.00
133_D154_T1.2101.00
327_I341_V1.2081.00
254_I257_L1.2041.00
345_D349_R1.1981.00
97_A379_S1.1981.00
287_V294_L1.1971.00
151_L221_F1.1901.00
167_L191_P1.1861.00
177_Q180_G1.1851.00
271_R276_L1.1791.00
348_G390_N1.1761.00
151_L236_A1.1711.00
202_Q206_T1.1711.00
103_P322_E1.1711.00
127_R131_F1.1651.00
378_V385_I1.1551.00
267_T275_T1.1501.00
149_P152_D1.1451.00
325_L373_E1.1441.00
184_R188_A1.1421.00
174_T179_E1.1411.00
268_V271_R1.1411.00
160_E164_E1.1391.00
324_M366_A1.1381.00
192_E196_R1.1361.00
198_L202_Q1.1321.00
267_T273_D1.1291.00
143_K305_E1.1261.00
230_A233_N1.1251.00
137_P236_A1.1221.00
212_A215_D1.1131.00
98_T374_G1.1121.00
91_N390_N1.1121.00
335_T338_E1.1041.00
123_I234_V1.0981.00
176_T180_G1.0941.00
165_Y198_L1.0901.00
344_V376_K1.0841.00
255_A294_L1.0821.00
168_L171_T1.0821.00
135_V225_A1.0771.00
329_S360_A1.0761.00
246_V266_L1.0731.00
113_N292_R1.0711.00
120_Q225_A1.0641.00
279_R299_E1.0571.00
335_T352_P1.0561.00
271_R274_K1.0541.00
123_I237_K1.0471.00
134_K225_A1.0461.00
129_A230_A1.0421.00
214_I238_I1.0411.00
114_V313_A1.0411.00
220_A237_K1.0371.00
343_T351_V1.0361.00
350_F391_I1.0201.00
221_F224_R1.0171.00
143_K215_D1.0171.00
114_V307_L1.0141.00
158_W162_Q1.0101.00
302_N305_E1.0061.00
332_L339_Q1.0051.00
151_L210_L1.0011.00
168_L195_I0.9981.00
360_A365_T0.9931.00
92_L389_A0.9911.00
280_K298_L0.9851.00
243_P301_D0.9841.00
229_I235_V0.9771.00
132_I229_I0.9651.00
246_V315_L0.9641.00
352_P387_S0.9641.00
182_L185_L0.9631.00
361_S364_V0.9631.00
367_L371_L0.9631.00
137_P221_F0.9621.00
330_Q380_S0.9581.00
332_L365_T0.9571.00
119_Y245_W0.9541.00
280_K296_L0.9491.00
184_R187_L0.9361.00
179_E182_L0.9331.00
133_D155_I0.9271.00
178_T181_I0.9271.00
101_R325_L0.9251.00
221_F236_A0.9221.00
155_I208_F0.9151.00
247_T297_R0.9141.00
284_L295_Q0.9081.00
339_Q357_V0.8991.00
344_V348_G0.8971.00
164_E168_L0.8951.00
357_V367_L0.8951.00
121_Y239_Q0.8931.00
172_G179_E0.8891.00
97_A100_T0.8891.00
100_T373_E0.8881.00
105_T318_N0.8871.00
107_A318_N0.8821.00
342_I385_I0.8811.00
353_K372_A0.8761.00
126_A229_I0.8761.00
245_W299_E0.8751.00
270_A274_K0.8741.00
103_P321_S0.8701.00
358_F365_T0.8701.00
288_D291_T0.8691.00
137_P218_I0.8681.00
140_V220_A0.8671.00
91_N392_S0.8621.00
122_A214_I0.8621.00
157_D204_I0.8611.00
348_G376_K0.8571.00
278_I300_V0.8561.00
94_V378_V0.8511.00
376_K395_L0.8441.00
159_V199_I0.8431.00
188_A191_P0.8421.00
244_V309_P0.8411.00
157_D200_A0.8391.00
203_K206_T0.8381.00
345_D348_G0.8351.00
344_V378_V0.8321.00
250_I296_L0.8261.00
216_G238_I0.8261.00
345_D353_K0.8231.00
173_G177_Q0.8151.00
187_L191_P0.8131.00
201_T205_Q0.8121.00
131_F228_N0.8061.00
333_I340_R0.8051.00
151_L235_V0.7991.00
242_D303_A0.7981.00
180_G184_R0.7981.00
99_V371_L0.7981.00
126_A210_L0.7981.00
350_F390_N0.7891.00
119_Y243_P0.7811.00
169_R192_E0.7801.00
267_T314_W0.7781.00
171_T191_P0.7771.00
342_I387_S0.7761.00
385_I391_I0.7731.00
142_D148_T0.7721.00
270_A273_D0.7641.00
386_D389_A0.7581.00
132_I235_V0.7581.00
97_A328_P0.7581.00
282_T297_R0.7561.00
110_F250_I0.7541.00
183_E187_L0.7541.00
335_T340_R0.7531.00
101_R372_A0.7531.00
348_G393_G0.7531.00
133_D152_D0.7461.00
124_V214_I0.7431.00
119_Y240_G0.7421.00
125_Q233_N0.7401.00
203_K207_R0.7401.00
332_L357_V0.7381.00
248_A317_L0.7341.00
342_I350_F0.7331.00
283_L296_L0.7311.00
327_I332_L0.7301.00
120_Q224_R0.7301.00
108_Q254_I0.7301.00
171_T187_L0.7301.00
162_Q198_L0.7261.00
158_W202_Q0.7251.00
96_T379_S0.7241.00
270_A276_L0.7241.00
174_T177_Q0.7211.00
166_L193_A0.7201.00
173_G179_E0.7191.00
343_T377_V0.7191.00
241_M309_P0.7171.00
152_D155_I0.7151.00
358_F370_G0.7141.00
112_A313_A0.7141.00
135_V151_L0.7131.00
163_S170_E0.7121.00
188_A192_E0.7121.00
108_Q319_T0.7121.00
95_K394_A0.7101.00
266_L315_L0.7101.00
155_I209_T0.7081.00
269_P314_W0.7031.00
250_I258_V0.7001.00
191_P194_D0.6941.00
104_L361_S0.6901.00
110_F248_A0.6821.00
175_A182_L0.6821.00
116_Y309_P0.6781.00
246_V300_V0.6771.00
167_L170_E0.6751.00
174_T181_I0.6741.00
273_D276_L0.6711.00
342_I352_P0.6711.00
156_P203_K0.6711.00
222_D237_K0.6691.00
350_F385_I0.6671.00
89_T390_N0.6671.00
185_L192_E0.6671.00
161_A164_E0.6671.00
258_V280_K0.6661.00
272_P276_L0.6661.00
266_L270_A0.6661.00
119_Y299_E0.6651.00
161_A195_I0.6641.00
164_E191_P0.6641.00
99_V375_E0.6611.00
242_D305_E0.6601.00
191_P195_I0.6571.00
100_T364_V0.6561.00
219_T284_L0.6551.00
353_K370_G0.6551.00
98_T101_R0.6551.00
327_I331_A0.6551.00
244_V302_N0.6531.00
332_L355_V0.6511.00
346_A376_K0.6471.00
97_A327_I0.6471.00
155_I205_Q0.6461.00
260_D283_L0.6461.00
341_V377_V0.6461.00
173_G181_I0.6451.00
244_V300_V0.6451.00
166_L170_E0.6451.00
279_R301_D0.6421.00
157_D161_A0.6401.00
97_A364_V0.6391.00
358_F367_L0.6371.00
181_I184_R0.6341.00
174_T180_G0.6321.00
281_W297_R0.6291.00
136_Y148_T0.6241.00
357_V368_R0.6231.00
140_V221_F0.6211.00
177_Q181_I0.6201.00
344_V350_F0.6201.00
269_P272_P0.6191.00
376_K393_G0.6181.00
133_D136_Y0.6161.00
201_T204_I0.6161.00
341_V371_L0.6151.00
259_K262_S0.6151.00
263_Q318_N0.6121.00
129_A228_N0.6121.00
346_A349_R0.6111.00
162_Q169_R0.6091.00
173_G178_T0.6081.00
248_A264_F0.6071.00
194_D197_R0.6071.00
340_R354_R0.6071.00
221_F225_A0.6061.00
185_L188_A0.6041.00
345_D375_E0.6041.00
239_Q285_P0.6000.99
99_V377_V0.5970.99
175_A178_T0.5970.99
120_Q214_I0.5970.99
158_W165_Y0.5950.99
122_A238_I0.5930.99
165_Y196_R0.5930.99
125_Q228_N0.5930.99
159_V163_S0.5920.99
172_G180_G0.5900.99
132_I153_L0.5890.99
143_K241_M0.5880.99
104_L366_A0.5870.99
183_E186_R0.5840.99
123_I227_M0.5820.99
192_E198_L0.5820.99
342_I378_V0.5780.99
94_V391_I0.5780.99
112_A246_V0.5750.99
249_A288_D0.5720.99
104_L326_L0.5710.99
114_V300_V0.5710.99
241_M305_E0.5710.99
110_F254_I0.5690.99
272_P275_T0.5670.99
114_V268_V0.5660.99
223_L229_I0.5660.99
106_F257_L0.5650.99
222_D234_V0.5650.99
331_A379_S0.5640.99
147_G215_D0.5640.99
223_L227_M0.5640.99
341_V370_G0.5640.99
220_A282_T0.5620.99
122_A213_P0.5620.99
126_A151_L0.5600.99
274_K304_D0.5590.99
217_V241_M0.5590.99
264_F270_A0.5590.99
266_L269_P0.5560.99
124_V238_I0.5540.99
287_V292_R0.5540.99
173_G176_T0.5530.99
206_T211_K0.5520.99
189_G196_R0.5500.99
97_A101_R0.5460.99
162_Q165_Y0.5450.99
190_M194_D0.5450.99
369_S372_A0.5450.99
361_S366_A0.5440.99
105_T321_S0.5440.99
276_L306_A0.5420.99
350_F387_S0.5420.99
364_V368_R0.5410.99
144_V150_L0.5410.99
163_S166_L0.5400.99
318_N321_S0.5380.99
160_E167_L0.5380.99
172_G178_T0.5380.99
106_F359_Q0.5370.99
341_V375_E0.5370.99
269_P273_D0.5370.99
288_D295_Q0.5340.99
116_Y121_Y0.5340.99
133_D225_A0.5320.99
325_L375_E0.5310.99
189_G192_E0.5310.99
234_V237_K0.5290.99
327_I365_T0.5280.99
199_I203_K0.5270.99
163_S171_T0.5260.99
125_Q227_M0.5250.99
276_L304_D0.5250.99
135_V221_F0.5240.99
161_A201_T0.5240.99
333_I342_I0.5220.99
116_Y119_Y0.5210.99
241_M302_N0.5200.99
108_Q317_L0.5200.99
331_A341_V0.5200.99
300_V307_L0.5200.99
160_E197_R0.5170.98
172_G176_T0.5170.98
116_Y120_Q0.5160.98
333_I382_L0.5140.98
264_F317_L0.5130.98
266_L278_I0.5130.98
328_P331_A0.5110.98
144_V212_A0.5110.98
164_E194_D0.5110.98
126_A129_A0.5100.98
132_I135_V0.5090.98
99_V327_I0.5090.98
176_T179_E0.5080.98
212_A218_I0.5080.98
210_L235_V0.5070.98
326_L366_A0.5050.98
125_Q234_V0.5030.98
268_V307_L0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ne5B 3 0.7936 100 0.141 Contact Map
3lnnA 6 0.7666 100 0.352 Contact Map
1vf7A 5 0.516 100 0.382 Contact Map
4dk0A 5 0.688 100 0.401 Contact Map
4l8jA 2 0.7322 100 0.404 Contact Map
4kkuA 1 0.6462 100 0.414 Contact Map
3fppA 3 0.5504 100 0.418 Contact Map
2f1mA 2 0.516 100 0.548 Contact Map
4tkoB 1 0.5184 100 0.597 Contact Map
2k32A 1 0.2826 99.8 0.77 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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