CUSB - ECOLI - GREMLIN Contacts

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CUSB - Cation efflux system protein CusB

UniProt:	P77239	Sequences: MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANISGALERMRSESATHAH	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG14235
Length:	407 (311)
Sequences:	11789 (9022)
Seq/√Len:	511.6

ECOLI - CUSB MDTA MDTE ACRA ACRE MACA
BACSU - YKNX YVRP

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
345_D	351_V	3.400	1.00
134_K	152_D	3.107	1.00
149_P	209_T	3.052	1.00
356_A	369_S	2.994	1.00
98_T	376_K	2.789	1.00
353_K	375_E	2.659	1.00
145_Q	215_D	2.554	1.00
340_R	352_P	2.452	1.00
343_T	353_K	2.428	1.00
162_Q	202_Q	2.405	1.00
281_W	299_E	2.268	1.00
102_G	364_V	2.226	1.00
159_V	162_Q	2.194	1.00
263_Q	277_T	2.194	1.00
114_V	246_V	2.188	1.00
101_R	373_E	2.182	1.00
278_I	298_L	2.150	1.00
115_S	247_T	2.150	1.00
152_D	209_T	2.136	1.00
349_R	390_N	2.102	1.00
158_W	198_L	2.081	1.00
145_Q	148_T	2.073	1.00
265_T	277_T	2.071	1.00
149_P	211_K	2.025	1.00
329_S	365_T	2.014	1.00
326_L	364_V	2.000	1.00
136_Y	149_P	1.993	1.00
136_Y	152_D	1.971	1.00
248_A	315_L	1.952	1.00
344_V	391_I	1.908	1.00
338_E	354_R	1.902	1.00
101_R	323_P	1.891	1.00
169_R	196_R	1.877	1.00
143_K	217_V	1.869	1.00
372_A	375_E	1.867	1.00
193_A	196_R	1.864	1.00
212_A	238_I	1.861	1.00
93_G	392_S	1.852	1.00
351_V	387_S	1.823	1.00
167_L	171_T	1.819	1.00
274_K	306_A	1.811	1.00
107_A	316_Q	1.810	1.00
114_V	244_V	1.795	1.00
146_K	215_D	1.793	1.00
356_A	368_R	1.785	1.00
120_Q	240_G	1.774	1.00
358_F	368_R	1.755	1.00
185_L	189_G	1.740	1.00
182_L	186_R	1.738	1.00
175_A	179_E	1.710	1.00
266_L	300_V	1.709	1.00
102_G	326_L	1.702	1.00
127_R	207_R	1.690	1.00
174_T	178_T	1.687	1.00
358_F	366_A	1.675	1.00
96_T	393_G	1.674	1.00
264_F	298_L	1.673	1.00
186_R	189_G	1.662	1.00
355_V	367_L	1.661	1.00
218_I	236_A	1.659	1.00
359_Q	366_A	1.656	1.00
162_Q	166_L	1.641	1.00
166_L	169_R	1.639	1.00
99_V	325_L	1.638	1.00
248_A	298_L	1.621	1.00
165_Y	192_E	1.611	1.00
109_S	316_Q	1.610	1.00
350_F	378_V	1.605	1.00
132_I	223_L	1.595	1.00
341_V	355_V	1.573	1.00
263_Q	279_R	1.548	1.00
268_V	313_A	1.546	1.00
95_K	380_S	1.543	1.00
96_T	344_V	1.530	1.00
265_T	275_T	1.528	1.00
357_V	365_T	1.527	1.00
245_W	297_R	1.526	1.00
196_R	199_I	1.492	1.00
99_V	374_G	1.479	1.00
178_T	182_L	1.477	1.00
199_I	202_Q	1.473	1.00
329_S	363_G	1.463	1.00
343_T	375_E	1.436	1.00
109_S	314_W	1.436	1.00
327_I	377_V	1.435	1.00
96_T	376_K	1.432	1.00
189_G	193_A	1.406	1.00
247_T	295_Q	1.395	1.00
132_I	151_L	1.392	1.00
334_D	339_Q	1.385	1.00
165_Y	169_R	1.382	1.00
200_A	204_I	1.369	1.00
249_A	295_Q	1.364	1.00
251_P	254_I	1.359	1.00
93_G	394_A	1.354	1.00
129_A	231_K	1.341	1.00
121_Y	237_K	1.333	1.00
265_T	318_N	1.309	1.00
243_P	299_E	1.299	1.00
240_G	243_P	1.299	1.00
219_T	239_Q	1.290	1.00
267_T	316_Q	1.286	1.00
246_V	313_A	1.281	1.00
265_T	316_Q	1.259	1.00
157_D	160_E	1.259	1.00
103_P	323_P	1.238	1.00
137_P	148_T	1.227	1.00
130_G	153_L	1.226	1.00
150_L	236_A	1.223	1.00
164_E	167_L	1.218	1.00
133_D	154_T	1.210	1.00
327_I	341_V	1.208	1.00
254_I	257_L	1.204	1.00
345_D	349_R	1.198	1.00
97_A	379_S	1.198	1.00
287_V	294_L	1.197	1.00
151_L	221_F	1.190	1.00
167_L	191_P	1.186	1.00
177_Q	180_G	1.185	1.00
271_R	276_L	1.179	1.00
348_G	390_N	1.176	1.00
151_L	236_A	1.171	1.00
202_Q	206_T	1.171	1.00
103_P	322_E	1.171	1.00
127_R	131_F	1.165	1.00
378_V	385_I	1.155	1.00
267_T	275_T	1.150	1.00
149_P	152_D	1.145	1.00
325_L	373_E	1.144	1.00
184_R	188_A	1.142	1.00
174_T	179_E	1.141	1.00
268_V	271_R	1.141	1.00
160_E	164_E	1.139	1.00
324_M	366_A	1.138	1.00
192_E	196_R	1.136	1.00
198_L	202_Q	1.132	1.00
267_T	273_D	1.129	1.00
143_K	305_E	1.126	1.00
230_A	233_N	1.125	1.00
137_P	236_A	1.122	1.00
212_A	215_D	1.113	1.00
98_T	374_G	1.112	1.00
91_N	390_N	1.112	1.00
335_T	338_E	1.104	1.00
123_I	234_V	1.098	1.00
176_T	180_G	1.094	1.00
165_Y	198_L	1.090	1.00
344_V	376_K	1.084	1.00
255_A	294_L	1.082	1.00
168_L	171_T	1.082	1.00
135_V	225_A	1.077	1.00
329_S	360_A	1.076	1.00
246_V	266_L	1.073	1.00
113_N	292_R	1.071	1.00
120_Q	225_A	1.064	1.00
279_R	299_E	1.057	1.00
335_T	352_P	1.056	1.00
271_R	274_K	1.054	1.00
123_I	237_K	1.047	1.00
134_K	225_A	1.046	1.00
129_A	230_A	1.042	1.00
214_I	238_I	1.041	1.00
114_V	313_A	1.041	1.00
220_A	237_K	1.037	1.00
343_T	351_V	1.036	1.00
350_F	391_I	1.020	1.00
221_F	224_R	1.017	1.00
143_K	215_D	1.017	1.00
114_V	307_L	1.014	1.00
158_W	162_Q	1.010	1.00
302_N	305_E	1.006	1.00
332_L	339_Q	1.005	1.00
151_L	210_L	1.001	1.00
168_L	195_I	0.998	1.00
360_A	365_T	0.993	1.00
92_L	389_A	0.991	1.00
280_K	298_L	0.985	1.00
243_P	301_D	0.984	1.00
229_I	235_V	0.977	1.00
132_I	229_I	0.965	1.00
246_V	315_L	0.964	1.00
352_P	387_S	0.964	1.00
182_L	185_L	0.963	1.00
361_S	364_V	0.963	1.00
367_L	371_L	0.963	1.00
137_P	221_F	0.962	1.00
330_Q	380_S	0.958	1.00
332_L	365_T	0.957	1.00
119_Y	245_W	0.954	1.00
280_K	296_L	0.949	1.00
184_R	187_L	0.936	1.00
179_E	182_L	0.933	1.00
133_D	155_I	0.927	1.00
178_T	181_I	0.927	1.00
101_R	325_L	0.925	1.00
221_F	236_A	0.922	1.00
155_I	208_F	0.915	1.00
247_T	297_R	0.914	1.00
284_L	295_Q	0.908	1.00
339_Q	357_V	0.899	1.00
344_V	348_G	0.897	1.00
164_E	168_L	0.895	1.00
357_V	367_L	0.895	1.00
121_Y	239_Q	0.893	1.00
172_G	179_E	0.889	1.00
97_A	100_T	0.889	1.00
100_T	373_E	0.888	1.00
105_T	318_N	0.887	1.00
107_A	318_N	0.882	1.00
342_I	385_I	0.881	1.00
353_K	372_A	0.876	1.00
126_A	229_I	0.876	1.00
245_W	299_E	0.875	1.00
270_A	274_K	0.874	1.00
103_P	321_S	0.870	1.00
358_F	365_T	0.870	1.00
288_D	291_T	0.869	1.00
137_P	218_I	0.868	1.00
140_V	220_A	0.867	1.00
91_N	392_S	0.862	1.00
122_A	214_I	0.862	1.00
157_D	204_I	0.861	1.00
348_G	376_K	0.857	1.00
278_I	300_V	0.856	1.00
94_V	378_V	0.851	1.00
376_K	395_L	0.844	1.00
159_V	199_I	0.843	1.00
188_A	191_P	0.842	1.00
244_V	309_P	0.841	1.00
157_D	200_A	0.839	1.00
203_K	206_T	0.838	1.00
345_D	348_G	0.835	1.00
344_V	378_V	0.832	1.00
250_I	296_L	0.826	1.00
216_G	238_I	0.826	1.00
345_D	353_K	0.823	1.00
173_G	177_Q	0.815	1.00
187_L	191_P	0.813	1.00
201_T	205_Q	0.812	1.00
131_F	228_N	0.806	1.00
333_I	340_R	0.805	1.00
151_L	235_V	0.799	1.00
242_D	303_A	0.798	1.00
180_G	184_R	0.798	1.00
99_V	371_L	0.798	1.00
126_A	210_L	0.798	1.00
350_F	390_N	0.789	1.00
119_Y	243_P	0.781	1.00
169_R	192_E	0.780	1.00
267_T	314_W	0.778	1.00
171_T	191_P	0.777	1.00
342_I	387_S	0.776	1.00
385_I	391_I	0.773	1.00
142_D	148_T	0.772	1.00
270_A	273_D	0.764	1.00
386_D	389_A	0.758	1.00
132_I	235_V	0.758	1.00
97_A	328_P	0.758	1.00
282_T	297_R	0.756	1.00
110_F	250_I	0.754	1.00
183_E	187_L	0.754	1.00
335_T	340_R	0.753	1.00
101_R	372_A	0.753	1.00
348_G	393_G	0.753	1.00
133_D	152_D	0.746	1.00
124_V	214_I	0.743	1.00
119_Y	240_G	0.742	1.00
125_Q	233_N	0.740	1.00
203_K	207_R	0.740	1.00
332_L	357_V	0.738	1.00
248_A	317_L	0.734	1.00
342_I	350_F	0.733	1.00
283_L	296_L	0.731	1.00
327_I	332_L	0.730	1.00
120_Q	224_R	0.730	1.00
108_Q	254_I	0.730	1.00
171_T	187_L	0.730	1.00
162_Q	198_L	0.726	1.00
158_W	202_Q	0.725	1.00
96_T	379_S	0.724	1.00
270_A	276_L	0.724	1.00
174_T	177_Q	0.721	1.00
166_L	193_A	0.720	1.00
173_G	179_E	0.719	1.00
343_T	377_V	0.719	1.00
241_M	309_P	0.717	1.00
152_D	155_I	0.715	1.00
358_F	370_G	0.714	1.00
112_A	313_A	0.714	1.00
135_V	151_L	0.713	1.00
163_S	170_E	0.712	1.00
188_A	192_E	0.712	1.00
108_Q	319_T	0.712	1.00
95_K	394_A	0.710	1.00
266_L	315_L	0.710	1.00
155_I	209_T	0.708	1.00
269_P	314_W	0.703	1.00
250_I	258_V	0.700	1.00
191_P	194_D	0.694	1.00
104_L	361_S	0.690	1.00
110_F	248_A	0.682	1.00
175_A	182_L	0.682	1.00
116_Y	309_P	0.678	1.00
246_V	300_V	0.677	1.00
167_L	170_E	0.675	1.00
174_T	181_I	0.674	1.00
273_D	276_L	0.671	1.00
342_I	352_P	0.671	1.00
156_P	203_K	0.671	1.00
222_D	237_K	0.669	1.00
350_F	385_I	0.667	1.00
89_T	390_N	0.667	1.00
185_L	192_E	0.667	1.00
161_A	164_E	0.667	1.00
258_V	280_K	0.666	1.00
272_P	276_L	0.666	1.00
266_L	270_A	0.666	1.00
119_Y	299_E	0.665	1.00
161_A	195_I	0.664	1.00
164_E	191_P	0.664	1.00
99_V	375_E	0.661	1.00
242_D	305_E	0.660	1.00
191_P	195_I	0.657	1.00
100_T	364_V	0.656	1.00
219_T	284_L	0.655	1.00
353_K	370_G	0.655	1.00
98_T	101_R	0.655	1.00
327_I	331_A	0.655	1.00
244_V	302_N	0.653	1.00
332_L	355_V	0.651	1.00
346_A	376_K	0.647	1.00
97_A	327_I	0.647	1.00
155_I	205_Q	0.646	1.00
260_D	283_L	0.646	1.00
341_V	377_V	0.646	1.00
173_G	181_I	0.645	1.00
244_V	300_V	0.645	1.00
166_L	170_E	0.645	1.00
279_R	301_D	0.642	1.00
157_D	161_A	0.640	1.00
97_A	364_V	0.639	1.00
358_F	367_L	0.637	1.00
181_I	184_R	0.634	1.00
174_T	180_G	0.632	1.00
281_W	297_R	0.629	1.00
136_Y	148_T	0.624	1.00
357_V	368_R	0.623	1.00
140_V	221_F	0.621	1.00
177_Q	181_I	0.620	1.00
344_V	350_F	0.620	1.00
269_P	272_P	0.619	1.00
376_K	393_G	0.618	1.00
133_D	136_Y	0.616	1.00
201_T	204_I	0.616	1.00
341_V	371_L	0.615	1.00
259_K	262_S	0.615	1.00
263_Q	318_N	0.612	1.00
129_A	228_N	0.612	1.00
346_A	349_R	0.611	1.00
162_Q	169_R	0.609	1.00
173_G	178_T	0.608	1.00
248_A	264_F	0.607	1.00
194_D	197_R	0.607	1.00
340_R	354_R	0.607	1.00
221_F	225_A	0.606	1.00
185_L	188_A	0.604	1.00
345_D	375_E	0.604	1.00
239_Q	285_P	0.600	0.99
99_V	377_V	0.597	0.99
175_A	178_T	0.597	0.99
120_Q	214_I	0.597	0.99
158_W	165_Y	0.595	0.99
122_A	238_I	0.593	0.99
165_Y	196_R	0.593	0.99
125_Q	228_N	0.593	0.99
159_V	163_S	0.592	0.99
172_G	180_G	0.590	0.99
132_I	153_L	0.589	0.99
143_K	241_M	0.588	0.99
104_L	366_A	0.587	0.99
183_E	186_R	0.584	0.99
123_I	227_M	0.582	0.99
192_E	198_L	0.582	0.99
342_I	378_V	0.578	0.99
94_V	391_I	0.578	0.99
112_A	246_V	0.575	0.99
249_A	288_D	0.572	0.99
104_L	326_L	0.571	0.99
114_V	300_V	0.571	0.99
241_M	305_E	0.571	0.99
110_F	254_I	0.569	0.99
272_P	275_T	0.567	0.99
114_V	268_V	0.566	0.99
223_L	229_I	0.566	0.99
106_F	257_L	0.565	0.99
222_D	234_V	0.565	0.99
331_A	379_S	0.564	0.99
147_G	215_D	0.564	0.99
223_L	227_M	0.564	0.99
341_V	370_G	0.564	0.99
220_A	282_T	0.562	0.99
122_A	213_P	0.562	0.99
126_A	151_L	0.560	0.99
274_K	304_D	0.559	0.99
217_V	241_M	0.559	0.99
264_F	270_A	0.559	0.99
266_L	269_P	0.556	0.99
124_V	238_I	0.554	0.99
287_V	292_R	0.554	0.99
173_G	176_T	0.553	0.99
206_T	211_K	0.552	0.99
189_G	196_R	0.550	0.99
97_A	101_R	0.546	0.99
162_Q	165_Y	0.545	0.99
190_M	194_D	0.545	0.99
369_S	372_A	0.545	0.99
361_S	366_A	0.544	0.99
105_T	321_S	0.544	0.99
276_L	306_A	0.542	0.99
350_F	387_S	0.542	0.99
364_V	368_R	0.541	0.99
144_V	150_L	0.541	0.99
163_S	166_L	0.540	0.99
318_N	321_S	0.538	0.99
160_E	167_L	0.538	0.99
172_G	178_T	0.538	0.99
106_F	359_Q	0.537	0.99
341_V	375_E	0.537	0.99
269_P	273_D	0.537	0.99
288_D	295_Q	0.534	0.99
116_Y	121_Y	0.534	0.99
133_D	225_A	0.532	0.99
325_L	375_E	0.531	0.99
189_G	192_E	0.531	0.99
234_V	237_K	0.529	0.99
327_I	365_T	0.528	0.99
199_I	203_K	0.527	0.99
163_S	171_T	0.526	0.99
125_Q	227_M	0.525	0.99
276_L	304_D	0.525	0.99
135_V	221_F	0.524	0.99
161_A	201_T	0.524	0.99
333_I	342_I	0.522	0.99
116_Y	119_Y	0.521	0.99
241_M	302_N	0.520	0.99
108_Q	317_L	0.520	0.99
331_A	341_V	0.520	0.99
300_V	307_L	0.520	0.99
160_E	197_R	0.517	0.98
172_G	176_T	0.517	0.98
116_Y	120_Q	0.516	0.98
333_I	382_L	0.514	0.98
264_F	317_L	0.513	0.98
266_L	278_I	0.513	0.98
328_P	331_A	0.511	0.98
144_V	212_A	0.511	0.98
164_E	194_D	0.511	0.98
126_A	129_A	0.510	0.98
132_I	135_V	0.509	0.98
99_V	327_I	0.509	0.98
176_T	179_E	0.508	0.98
212_A	218_I	0.508	0.98
210_L	235_V	0.507	0.98
326_L	366_A	0.505	0.98
125_Q	234_V	0.503	0.98
268_V	307_L	0.500	0.98

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3ne5B	3	0.7936	100	0.141	Contact Map
3lnnA	6	0.7666	100	0.352	Contact Map
1vf7A	5	0.516	100	0.382	Contact Map
4dk0A	5	0.688	100	0.401	Contact Map
4l8jA	2	0.7322	100	0.404	Contact Map
4kkuA	1	0.6462	100	0.414	Contact Map
3fppA	3	0.5504	100	0.418	Contact Map
2f1mA	2	0.516	100	0.548	Contact Map
4tkoB	1	0.5184	100	0.597	Contact Map
2k32A	1	0.2826	99.8	0.77	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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