GCS2 - ECOLI - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

GCS2 - Putative glutamate--cysteine ligase 2

UniProt:	P77213	Sequences: MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNKITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPFQKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPHFIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMIDSIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKDYLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEALHRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13644
Length:	372 (348)
Sequences:	3217 (2101)
Seq/√Len:	112.6

ECOLI - GCS2
HALSA - GCS2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
175_D	349_R	5.697	1.00
28_Y	363_K	4.337	1.00
64_D	75_Q	4.173	1.00
69_I	255_G	3.570	1.00
16_I	72_A	3.386	1.00
25_P	93_E	2.912	1.00
73_A	251_V	2.812	1.00
238_V	257_I	2.795	1.00
13_T	140_G	2.737	1.00
165_S	253_M	2.693	1.00
358_L	361_L	2.662	1.00
305_R	310_D	2.645	1.00
68_D	71_Q	2.624	1.00
66_C	71_Q	2.620	1.00
67_R	71_Q	2.611	1.00
266_T	324_K	2.563	1.00
31_S	92_L	2.528	1.00
323_H	328_S	2.526	1.00
179_A	346_Q	2.507	1.00
179_A	349_R	2.489	1.00
256_L	318_I	2.484	1.00
112_D	117_Q	2.383	1.00
337_Q	341_G	2.326	1.00
358_L	362_V	2.307	1.00
200_N	203_Q	2.286	1.00
357_S	360_G	2.276	1.00
144_G	205_E	2.266	1.00
267_E	270_F	2.265	1.00
40_A	90_H	2.243	1.00
199_S	203_Q	2.209	1.00
40_A	89_D	2.203	1.00
308_T	312_L	2.168	1.00
209_R	213_Y	2.155	1.00
305_R	313_R	2.130	1.00
293_E	346_Q	2.116	1.00
170_Y	352_V	2.107	1.00
319_A	323_H	2.084	1.00
24_N	27_G	2.055	1.00
96_G	243_T	2.022	1.00
342_L	350_D	2.017	1.00
73_A	77_S	2.017	1.00
147_A	229_P	2.010	1.00
306_P	309_E	1.964	1.00
33_D	92_L	1.946	1.00
319_A	335_H	1.905	1.00
33_D	36_M	1.902	1.00
359_I	362_V	1.898	1.00
256_L	314_L	1.893	1.00
286_Q	290_Y	1.868	1.00
244_P	253_M	1.849	1.00
151_L	236_V	1.775	1.00
346_Q	350_D	1.758	1.00
295_V	304_R	1.733	1.00
124_G	128_Q	1.718	1.00
312_L	339_V	1.705	1.00
293_E	308_T	1.702	1.00
343_N	346_Q	1.686	1.00
252_N	334_L	1.685	1.00
295_V	306_P	1.673	1.00
157_F	260_T	1.659	1.00
336_R	340_S	1.657	1.00
269_P	272_H	1.657	1.00
179_A	345_A	1.642	1.00
12_F	262_H	1.642	1.00
355_G	360_G	1.640	1.00
263_W	321_S	1.630	1.00
76_F	247_L	1.630	1.00
66_C	75_Q	1.629	1.00
22_V	92_L	1.613	1.00
360_G	363_K	1.604	1.00
255_G	331_I	1.592	1.00
77_S	81_K	1.585	1.00
263_W	317_K	1.585	1.00
63_T	66_C	1.582	1.00
15_G	19_E	1.579	1.00
143_S	146_D	1.575	1.00
154_L	238_V	1.566	1.00
21_Q	133_F	1.561	1.00
50_V	83_V	1.544	1.00
26_P	91_H	1.541	1.00
246_T	249_H	1.529	1.00
335_H	339_V	1.521	1.00
100_H	108_Q	1.519	1.00
73_A	330_A	1.516	1.00
332_E	335_H	1.506	1.00
51_K	62_A	1.503	1.00
142_A	146_D	1.485	1.00
351_F	355_G	1.478	1.00
73_A	248_S	1.473	1.00
315_L	331_I	1.470	1.00
259_A	327_A	1.460	1.00
224_H	228_R	1.444	1.00
23_V	95_C	1.436	1.00
160_H	311_T	1.432	1.00
269_P	273_Q	1.409	1.00
104_K	107_R	1.401	1.00
219_S	222_D	1.388	1.00
276_D	279_L	1.386	1.00
135_Q	250_A	1.374	1.00
350_D	354_D	1.352	1.00
84_L	93_E	1.347	1.00
262_H	266_T	1.346	1.00
282_F	286_Q	1.328	1.00
70_N	74_G	1.321	1.00
99_T	171_M	1.318	1.00
201_W	205_E	1.315	1.00
34_S	38_I	1.308	1.00
74_G	78_A	1.300	0.99
59_L	83_V	1.298	0.99
98_G	243_T	1.298	0.99
259_A	322_A	1.297	0.99
249_H	347_L	1.291	0.99
259_A	321_S	1.288	0.99
171_M	176_T	1.288	0.99
286_Q	304_R	1.280	0.99
100_H	103_Q	1.278	0.99
154_L	261_A	1.266	0.99
16_I	254_A	1.265	0.99
207_L	210_C	1.249	0.99
308_T	339_V	1.244	0.99
21_Q	58_M	1.235	0.99
77_S	247_L	1.230	0.99
25_P	88_T	1.229	0.99
149_Y	269_P	1.225	0.99
227_I	230_S	1.219	0.99
286_Q	294_G	1.215	0.99
41_V	50_V	1.211	0.99
178_F	184_N	1.203	0.99
138_H	235_T	1.200	0.99
319_A	328_S	1.199	0.99
82_V	85_Q	1.191	0.99
315_L	335_H	1.178	0.99
71_Q	75_Q	1.159	0.99
259_A	318_I	1.155	0.99
161_F	257_I	1.140	0.99
137_V	254_A	1.140	0.99
203_Q	206_A	1.132	0.99
352_V	355_G	1.130	0.99
178_F	288_C	1.127	0.99
170_Y	349_R	1.124	0.98
65_V	140_G	1.111	0.98
342_L	347_L	1.110	0.98
305_R	309_E	1.092	0.98
35_S	39_D	1.085	0.98
13_T	65_V	1.079	0.98
167_A	322_A	1.077	0.98
115_R	219_S	1.076	0.98
320_P	324_K	1.075	0.98
20_M	61_L	1.075	0.98
316_E	319_A	1.075	0.98
175_D	353_A	1.065	0.98
129_Q	188_A	1.063	0.98
152_H	199_S	1.062	0.98
319_A	332_E	1.051	0.98
56_E	112_D	1.043	0.98
263_W	266_T	1.039	0.97
145_D	201_W	1.036	0.97
318_I	321_S	1.027	0.97
166_A	343_N	1.025	0.97
25_P	91_H	1.024	0.97
14_L	139_V	1.021	0.97
266_T	317_K	1.018	0.97
178_F	289_R	1.016	0.97
162_I	165_S	1.013	0.97
184_N	187_S	1.013	0.97
151_L	229_P	1.011	0.97
76_F	251_V	1.011	0.97
81_K	85_Q	1.011	0.97
38_I	52_H	1.009	0.97
165_S	241_M	1.002	0.97
328_S	332_E	1.001	0.97
69_I	251_V	0.998	0.97
163_A	287_A	0.997	0.97
262_H	324_K	0.995	0.97
225_W	230_S	0.985	0.96
187_S	281_K	0.983	0.96
162_I	240_V	0.982	0.96
225_W	228_R	0.981	0.96
279_L	297_T	0.980	0.96
22_V	94_I	0.979	0.96
85_Q	89_D	0.979	0.96
351_F	354_D	0.975	0.96
124_G	127_I	0.973	0.96
210_C	214_T	0.973	0.96
35_S	52_H	0.969	0.96
225_W	229_P	0.966	0.96
322_A	331_I	0.960	0.96
74_G	77_S	0.956	0.96
175_D	179_A	0.943	0.96
323_H	326_G	0.942	0.95
80_Q	83_V	0.939	0.95
21_Q	98_G	0.934	0.95
356_G	361_L	0.927	0.95
352_V	356_G	0.926	0.95
313_R	316_E	0.926	0.95
146_D	149_Y	0.922	0.95
132_V	186_F	0.922	0.95
350_D	353_A	0.915	0.95
197_W	200_N	0.914	0.95
114_E	117_Q	0.910	0.95
141_C	236_V	0.908	0.94
123_F	186_F	0.907	0.94
150_L	261_A	0.907	0.94
297_T	304_R	0.906	0.94
298_D	301_T	0.901	0.94
157_F	314_L	0.897	0.94
110_V	120_L	0.896	0.94
139_V	258_Q	0.890	0.94
162_I	183_P	0.883	0.94
30_L	100_H	0.880	0.94
19_E	60_E	0.880	0.94
354_D	360_G	0.878	0.93
153_G	264_L	0.876	0.93
153_G	267_E	0.875	0.93
266_T	321_S	0.874	0.93
226_D	284_R	0.873	0.93
312_L	316_E	0.873	0.93
30_L	103_Q	0.863	0.93
163_A	166_A	0.862	0.93
281_K	285_F	0.861	0.93
349_R	353_A	0.861	0.93
150_L	265_L	0.856	0.93
130_A	186_F	0.856	0.93
316_E	353_A	0.854	0.92
180_S	242_D	0.853	0.92
359_I	363_K	0.853	0.92
179_A	293_E	0.849	0.92
212_S	215_T	0.847	0.92
163_A	292_L	0.843	0.92
307_L	310_D	0.836	0.92
186_F	226_D	0.836	0.92
255_G	330_A	0.829	0.91
60_E	239_R	0.828	0.91
102_F	362_V	0.827	0.91
135_Q	243_T	0.824	0.91
142_A	265_L	0.822	0.91
97_G	167_A	0.821	0.91
356_G	359_I	0.821	0.91
86_A	90_H	0.819	0.91
150_L	236_V	0.816	0.91
73_A	247_L	0.816	0.91
198_V	204_F	0.815	0.91
96_G	247_L	0.815	0.91
154_L	257_I	0.814	0.91
167_A	343_N	0.814	0.91
55_T	132_V	0.811	0.90
36_M	39_D	0.811	0.90
263_W	324_K	0.805	0.90
61_L	83_V	0.803	0.90
258_Q	327_A	0.800	0.90
49_E	135_Q	0.795	0.90
166_A	179_A	0.793	0.89
290_Y	294_G	0.790	0.89
146_D	265_L	0.789	0.89
161_F	241_M	0.789	0.89
316_E	335_H	0.788	0.89
179_A	291_G	0.785	0.89
202_Q	206_A	0.785	0.89
149_Y	153_G	0.785	0.89
170_Y	175_D	0.780	0.89
352_V	360_G	0.778	0.88
298_D	313_R	0.778	0.88
227_I	236_V	0.777	0.88
241_M	253_M	0.772	0.88
348_M	351_F	0.772	0.88
137_V	241_M	0.768	0.88
262_H	321_S	0.765	0.88
224_H	230_S	0.764	0.88
161_F	238_V	0.762	0.87
171_M	185_I	0.762	0.87
296_I	306_P	0.762	0.87
196_P	199_S	0.761	0.87
263_W	267_E	0.759	0.87
309_E	312_L	0.759	0.87
49_E	61_L	0.753	0.87
54_I	119_T	0.752	0.87
330_A	337_Q	0.751	0.87
15_G	60_E	0.750	0.87
29_D	103_Q	0.749	0.87
262_H	325_I	0.738	0.86
97_G	348_M	0.738	0.86
147_A	204_F	0.734	0.85
252_N	331_I	0.732	0.85
329_S	336_R	0.728	0.85
321_S	325_I	0.725	0.85
154_L	236_V	0.724	0.85
220_I	231_P	0.724	0.85
176_T	181_S	0.721	0.84
206_A	210_C	0.720	0.84
181_S	185_I	0.719	0.84
54_I	130_A	0.713	0.84
248_S	333_A	0.711	0.84
357_S	361_L	0.710	0.83
204_F	208_F	0.710	0.83
251_V	330_A	0.709	0.83
89_D	350_D	0.708	0.83
18_L	80_Q	0.706	0.83
117_Q	121_E	0.703	0.83
247_L	251_V	0.701	0.83
287_A	307_L	0.700	0.83
199_S	206_A	0.700	0.83
49_E	64_D	0.700	0.83
285_F	289_R	0.699	0.83
55_T	131_T	0.699	0.83
159_P	242_D	0.699	0.83
76_F	135_Q	0.694	0.82
58_M	123_F	0.693	0.82
194_P	197_W	0.693	0.82
160_H	314_L	0.690	0.82
57_S	131_T	0.688	0.82
266_T	320_P	0.688	0.82
56_E	109_E	0.687	0.81
41_V	86_A	0.686	0.81
162_I	284_R	0.686	0.81
14_L	258_Q	0.679	0.81
107_R	121_E	0.678	0.81
256_L	331_I	0.674	0.80
76_F	80_Q	0.670	0.80
177_R	180_S	0.669	0.80
267_E	317_K	0.668	0.80
20_M	94_I	0.665	0.79
129_Q	171_M	0.664	0.79
296_I	305_R	0.661	0.79
39_D	42_K	0.659	0.79
78_A	81_K	0.658	0.79
329_S	332_E	0.656	0.78
162_I	287_A	0.655	0.78
72_A	224_H	0.655	0.78
54_I	111_C	0.655	0.78
158_V	238_V	0.654	0.78
152_H	203_Q	0.654	0.78
320_P	323_H	0.650	0.78
307_L	314_L	0.650	0.78
140_G	186_F	0.649	0.78
66_C	73_A	0.648	0.78
118_R	121_E	0.644	0.77
217_I	223_L	0.644	0.77
23_V	362_V	0.644	0.77
54_I	129_Q	0.644	0.77
321_S	324_K	0.643	0.77
88_T	93_E	0.643	0.77
167_A	321_S	0.642	0.77
131_T	186_F	0.641	0.77
336_R	339_V	0.641	0.77
207_L	212_S	0.641	0.77
219_S	223_L	0.640	0.77
312_L	335_H	0.639	0.77
60_E	237_E	0.639	0.77
178_F	181_S	0.638	0.76
73_A	329_S	0.633	0.76
316_E	320_P	0.632	0.76
287_A	296_I	0.631	0.76
162_I	166_A	0.628	0.75
135_Q	244_P	0.626	0.75
129_Q	185_I	0.625	0.75
36_M	90_H	0.625	0.75
105_W	108_Q	0.624	0.75
164_L	338_V	0.623	0.75
208_F	212_S	0.622	0.75
148_I	152_H	0.621	0.75
14_L	72_A	0.620	0.74
308_T	341_G	0.619	0.74
12_F	142_A	0.618	0.74
115_R	119_T	0.617	0.74
310_D	313_R	0.616	0.74
248_S	251_V	0.615	0.74
357_S	362_V	0.615	0.74
334_L	337_Q	0.613	0.74
353_A	356_G	0.613	0.74
322_A	327_A	0.612	0.73
16_I	63_T	0.609	0.73
129_Q	224_H	0.609	0.73
296_I	310_D	0.609	0.73
221_K	231_P	0.608	0.73
74_G	81_K	0.607	0.73
167_A	259_A	0.607	0.73
20_M	63_T	0.607	0.73
102_F	359_I	0.606	0.73
23_V	93_E	0.606	0.73
171_M	181_S	0.605	0.73
184_N	288_C	0.604	0.72
202_Q	205_E	0.603	0.72
85_Q	354_D	0.601	0.72
70_N	330_A	0.600	0.72
99_T	181_S	0.600	0.72
180_S	183_P	0.600	0.72
350_D	363_K	0.598	0.72
60_E	136_H	0.597	0.72
56_E	111_C	0.597	0.72
63_T	138_H	0.597	0.72
342_L	346_Q	0.594	0.71
186_F	192_N	0.593	0.71
137_V	258_Q	0.592	0.71
206_A	209_R	0.592	0.71
331_I	335_H	0.592	0.71
16_I	134_G	0.592	0.71
136_H	235_T	0.590	0.71
138_H	228_R	0.590	0.71
33_D	90_H	0.585	0.70
105_W	124_G	0.585	0.70
355_G	361_L	0.585	0.70
270_F	273_Q	0.584	0.70
127_I	189_F	0.583	0.70
224_H	285_F	0.583	0.70
139_V	257_I	0.582	0.70
137_V	238_V	0.582	0.70
96_G	244_P	0.581	0.70
327_A	331_I	0.581	0.70
148_I	211_L	0.580	0.69
314_L	321_S	0.580	0.69
342_L	345_A	0.580	0.69
58_M	133_F	0.580	0.69
169_P	180_S	0.579	0.69
183_P	288_C	0.579	0.69
151_L	155_S	0.578	0.69
19_E	239_R	0.577	0.69
356_G	360_G	0.577	0.69
39_D	363_K	0.576	0.69
251_V	254_A	0.575	0.69
259_A	331_I	0.573	0.69
56_E	62_A	0.573	0.69
333_A	337_Q	0.573	0.69
76_F	250_A	0.573	0.69
329_S	333_A	0.573	0.69
58_M	132_V	0.572	0.68
327_A	330_A	0.572	0.68
151_L	227_I	0.570	0.68
337_Q	343_N	0.569	0.68
196_P	277_Y	0.569	0.68
222_D	226_D	0.568	0.68
144_G	231_P	0.567	0.68
155_S	194_P	0.564	0.67
152_H	197_W	0.563	0.67
60_E	116_Y	0.561	0.67
66_C	72_A	0.561	0.67
55_T	110_V	0.560	0.67
318_I	331_I	0.560	0.67
119_T	123_F	0.559	0.67
136_H	140_G	0.559	0.67
149_Y	267_E	0.559	0.67
24_N	31_S	0.558	0.67
41_V	85_Q	0.558	0.67
351_F	360_G	0.558	0.67
98_G	260_T	0.554	0.66
115_R	193_G	0.554	0.66
20_M	83_V	0.553	0.66
136_H	239_R	0.553	0.66
178_F	285_F	0.552	0.66
89_D	312_L	0.552	0.66
287_A	291_G	0.551	0.66
136_H	245_L	0.551	0.66
29_D	107_R	0.550	0.65
54_I	186_F	0.549	0.65
115_R	239_R	0.549	0.65
110_V	117_Q	0.547	0.65
71_Q	74_G	0.547	0.65
53_D	136_H	0.544	0.65
22_V	87_A	0.544	0.65
99_T	185_I	0.543	0.64
213_Y	302_G	0.543	0.64
14_L	69_I	0.543	0.64
67_R	174_T	0.542	0.64
351_F	361_L	0.542	0.64
69_I	258_Q	0.540	0.64
59_L	81_K	0.539	0.64
124_G	177_R	0.539	0.64
277_Y	280_Y	0.539	0.64
69_I	260_T	0.538	0.64
160_H	310_D	0.536	0.63
352_V	361_L	0.536	0.63
224_H	229_P	0.535	0.63
194_P	281_K	0.535	0.63
130_A	189_F	0.534	0.63
175_D	289_R	0.534	0.63
289_R	293_E	0.534	0.63
352_V	357_S	0.534	0.63
198_V	203_Q	0.533	0.63
43_N	70_N	0.531	0.63
348_M	361_L	0.531	0.63
287_A	294_G	0.531	0.63
57_S	130_A	0.530	0.63
84_L	87_A	0.529	0.62
111_C	138_H	0.529	0.62
353_A	357_S	0.526	0.62
255_G	326_G	0.524	0.62
332_E	340_S	0.524	0.62
209_R	212_S	0.524	0.62
37_L	90_H	0.523	0.62
104_K	172_Q	0.523	0.62
55_T	130_A	0.523	0.62
331_I	334_L	0.521	0.61
145_D	353_A	0.520	0.61
51_K	113_N	0.520	0.61
132_V	185_I	0.520	0.61
80_Q	84_L	0.518	0.61
100_H	181_S	0.517	0.61
323_H	332_E	0.517	0.61
100_H	296_I	0.517	0.61
21_Q	95_C	0.515	0.60
39_D	292_L	0.514	0.60
221_K	224_H	0.513	0.60
293_E	341_G	0.512	0.60
53_D	60_E	0.511	0.60
244_P	250_A	0.511	0.60
136_H	243_T	0.511	0.60
58_M	62_A	0.508	0.59
125_Y	128_Q	0.508	0.59
141_C	229_P	0.508	0.59
143_S	147_A	0.507	0.59
162_I	180_S	0.506	0.59
143_S	312_L	0.506	0.59
22_V	31_S	0.505	0.59
341_G	346_Q	0.505	0.59
261_A	265_L	0.505	0.59
183_P	186_F	0.504	0.59
111_C	116_Y	0.503	0.59
227_I	233_F	0.502	0.58
292_L	338_V	0.502	0.58
54_I	110_V	0.502	0.58
61_L	79_M	0.502	0.58
28_Y	362_V	0.501	0.58
65_V	72_A	0.500	0.58

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1r8gA	2	0.9651	100	0.17	Contact Map
2gwdA	2	0.9597	100	0.221	Contact Map
3nztA	1	0.9624	100	0.371	Contact Map
1va6A	1	0.9543	100	0.394	Contact Map
3ln6A	2	0.9435	100	0.406	Contact Map
3ln7A	2	0.9462	100	0.414	Contact Map
3ig5A	1	0.9973	100	0.469	Contact Map
4hppA	3	0.7366	98.8	0.878	Contact Map
4baxA	5	0.5565	98.4	0.895	Contact Map
4is4A	4	0.5027	98.4	0.895	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0112 seconds.