GREMLIN Database
GCS2 - Putative glutamate--cysteine ligase 2
UniProt: P77213 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13644
Length: 372 (348)
Sequences: 3217 (2101)
Seq/√Len: 112.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
175_D349_R5.6971.00
28_Y363_K4.3371.00
64_D75_Q4.1731.00
69_I255_G3.5701.00
16_I72_A3.3861.00
25_P93_E2.9121.00
73_A251_V2.8121.00
238_V257_I2.7951.00
13_T140_G2.7371.00
165_S253_M2.6931.00
358_L361_L2.6621.00
305_R310_D2.6451.00
68_D71_Q2.6241.00
66_C71_Q2.6201.00
67_R71_Q2.6111.00
266_T324_K2.5631.00
31_S92_L2.5281.00
323_H328_S2.5261.00
179_A346_Q2.5071.00
179_A349_R2.4891.00
256_L318_I2.4841.00
112_D117_Q2.3831.00
337_Q341_G2.3261.00
358_L362_V2.3071.00
200_N203_Q2.2861.00
357_S360_G2.2761.00
144_G205_E2.2661.00
267_E270_F2.2651.00
40_A90_H2.2431.00
199_S203_Q2.2091.00
40_A89_D2.2031.00
308_T312_L2.1681.00
209_R213_Y2.1551.00
305_R313_R2.1301.00
293_E346_Q2.1161.00
170_Y352_V2.1071.00
319_A323_H2.0841.00
24_N27_G2.0551.00
96_G243_T2.0221.00
342_L350_D2.0171.00
73_A77_S2.0171.00
147_A229_P2.0101.00
306_P309_E1.9641.00
33_D92_L1.9461.00
319_A335_H1.9051.00
33_D36_M1.9021.00
359_I362_V1.8981.00
256_L314_L1.8931.00
286_Q290_Y1.8681.00
244_P253_M1.8491.00
151_L236_V1.7751.00
346_Q350_D1.7581.00
295_V304_R1.7331.00
124_G128_Q1.7181.00
312_L339_V1.7051.00
293_E308_T1.7021.00
343_N346_Q1.6861.00
252_N334_L1.6851.00
295_V306_P1.6731.00
157_F260_T1.6591.00
336_R340_S1.6571.00
269_P272_H1.6571.00
179_A345_A1.6421.00
12_F262_H1.6421.00
355_G360_G1.6401.00
263_W321_S1.6301.00
76_F247_L1.6301.00
66_C75_Q1.6291.00
22_V92_L1.6131.00
360_G363_K1.6041.00
255_G331_I1.5921.00
77_S81_K1.5851.00
263_W317_K1.5851.00
63_T66_C1.5821.00
15_G19_E1.5791.00
143_S146_D1.5751.00
154_L238_V1.5661.00
21_Q133_F1.5611.00
50_V83_V1.5441.00
26_P91_H1.5411.00
246_T249_H1.5291.00
335_H339_V1.5211.00
100_H108_Q1.5191.00
73_A330_A1.5161.00
332_E335_H1.5061.00
51_K62_A1.5031.00
142_A146_D1.4851.00
351_F355_G1.4781.00
73_A248_S1.4731.00
315_L331_I1.4701.00
259_A327_A1.4601.00
224_H228_R1.4441.00
23_V95_C1.4361.00
160_H311_T1.4321.00
269_P273_Q1.4091.00
104_K107_R1.4011.00
219_S222_D1.3881.00
276_D279_L1.3861.00
135_Q250_A1.3741.00
350_D354_D1.3521.00
84_L93_E1.3471.00
262_H266_T1.3461.00
282_F286_Q1.3281.00
70_N74_G1.3211.00
99_T171_M1.3181.00
201_W205_E1.3151.00
34_S38_I1.3081.00
74_G78_A1.3000.99
59_L83_V1.2980.99
98_G243_T1.2980.99
259_A322_A1.2970.99
249_H347_L1.2910.99
259_A321_S1.2880.99
171_M176_T1.2880.99
286_Q304_R1.2800.99
100_H103_Q1.2780.99
154_L261_A1.2660.99
16_I254_A1.2650.99
207_L210_C1.2490.99
308_T339_V1.2440.99
21_Q58_M1.2350.99
77_S247_L1.2300.99
25_P88_T1.2290.99
149_Y269_P1.2250.99
227_I230_S1.2190.99
286_Q294_G1.2150.99
41_V50_V1.2110.99
178_F184_N1.2030.99
138_H235_T1.2000.99
319_A328_S1.1990.99
82_V85_Q1.1910.99
315_L335_H1.1780.99
71_Q75_Q1.1590.99
259_A318_I1.1550.99
161_F257_I1.1400.99
137_V254_A1.1400.99
203_Q206_A1.1320.99
352_V355_G1.1300.99
178_F288_C1.1270.99
170_Y349_R1.1240.98
65_V140_G1.1110.98
342_L347_L1.1100.98
305_R309_E1.0920.98
35_S39_D1.0850.98
13_T65_V1.0790.98
167_A322_A1.0770.98
115_R219_S1.0760.98
320_P324_K1.0750.98
20_M61_L1.0750.98
316_E319_A1.0750.98
175_D353_A1.0650.98
129_Q188_A1.0630.98
152_H199_S1.0620.98
319_A332_E1.0510.98
56_E112_D1.0430.98
263_W266_T1.0390.97
145_D201_W1.0360.97
318_I321_S1.0270.97
166_A343_N1.0250.97
25_P91_H1.0240.97
14_L139_V1.0210.97
266_T317_K1.0180.97
178_F289_R1.0160.97
162_I165_S1.0130.97
184_N187_S1.0130.97
151_L229_P1.0110.97
76_F251_V1.0110.97
81_K85_Q1.0110.97
38_I52_H1.0090.97
165_S241_M1.0020.97
328_S332_E1.0010.97
69_I251_V0.9980.97
163_A287_A0.9970.97
262_H324_K0.9950.97
225_W230_S0.9850.96
187_S281_K0.9830.96
162_I240_V0.9820.96
225_W228_R0.9810.96
279_L297_T0.9800.96
22_V94_I0.9790.96
85_Q89_D0.9790.96
351_F354_D0.9750.96
124_G127_I0.9730.96
210_C214_T0.9730.96
35_S52_H0.9690.96
225_W229_P0.9660.96
322_A331_I0.9600.96
74_G77_S0.9560.96
175_D179_A0.9430.96
323_H326_G0.9420.95
80_Q83_V0.9390.95
21_Q98_G0.9340.95
356_G361_L0.9270.95
352_V356_G0.9260.95
313_R316_E0.9260.95
146_D149_Y0.9220.95
132_V186_F0.9220.95
350_D353_A0.9150.95
197_W200_N0.9140.95
114_E117_Q0.9100.95
141_C236_V0.9080.94
123_F186_F0.9070.94
150_L261_A0.9070.94
297_T304_R0.9060.94
298_D301_T0.9010.94
157_F314_L0.8970.94
110_V120_L0.8960.94
139_V258_Q0.8900.94
162_I183_P0.8830.94
30_L100_H0.8800.94
19_E60_E0.8800.94
354_D360_G0.8780.93
153_G264_L0.8760.93
153_G267_E0.8750.93
266_T321_S0.8740.93
226_D284_R0.8730.93
312_L316_E0.8730.93
30_L103_Q0.8630.93
163_A166_A0.8620.93
281_K285_F0.8610.93
349_R353_A0.8610.93
150_L265_L0.8560.93
130_A186_F0.8560.93
316_E353_A0.8540.92
180_S242_D0.8530.92
359_I363_K0.8530.92
179_A293_E0.8490.92
212_S215_T0.8470.92
163_A292_L0.8430.92
307_L310_D0.8360.92
186_F226_D0.8360.92
255_G330_A0.8290.91
60_E239_R0.8280.91
102_F362_V0.8270.91
135_Q243_T0.8240.91
142_A265_L0.8220.91
97_G167_A0.8210.91
356_G359_I0.8210.91
86_A90_H0.8190.91
150_L236_V0.8160.91
73_A247_L0.8160.91
198_V204_F0.8150.91
96_G247_L0.8150.91
154_L257_I0.8140.91
167_A343_N0.8140.91
55_T132_V0.8110.90
36_M39_D0.8110.90
263_W324_K0.8050.90
61_L83_V0.8030.90
258_Q327_A0.8000.90
49_E135_Q0.7950.90
166_A179_A0.7930.89
290_Y294_G0.7900.89
146_D265_L0.7890.89
161_F241_M0.7890.89
316_E335_H0.7880.89
179_A291_G0.7850.89
202_Q206_A0.7850.89
149_Y153_G0.7850.89
170_Y175_D0.7800.89
352_V360_G0.7780.88
298_D313_R0.7780.88
227_I236_V0.7770.88
241_M253_M0.7720.88
348_M351_F0.7720.88
137_V241_M0.7680.88
262_H321_S0.7650.88
224_H230_S0.7640.88
161_F238_V0.7620.87
171_M185_I0.7620.87
296_I306_P0.7620.87
196_P199_S0.7610.87
263_W267_E0.7590.87
309_E312_L0.7590.87
49_E61_L0.7530.87
54_I119_T0.7520.87
330_A337_Q0.7510.87
15_G60_E0.7500.87
29_D103_Q0.7490.87
262_H325_I0.7380.86
97_G348_M0.7380.86
147_A204_F0.7340.85
252_N331_I0.7320.85
329_S336_R0.7280.85
321_S325_I0.7250.85
154_L236_V0.7240.85
220_I231_P0.7240.85
176_T181_S0.7210.84
206_A210_C0.7200.84
181_S185_I0.7190.84
54_I130_A0.7130.84
248_S333_A0.7110.84
357_S361_L0.7100.83
204_F208_F0.7100.83
251_V330_A0.7090.83
89_D350_D0.7080.83
18_L80_Q0.7060.83
117_Q121_E0.7030.83
247_L251_V0.7010.83
287_A307_L0.7000.83
199_S206_A0.7000.83
49_E64_D0.7000.83
285_F289_R0.6990.83
55_T131_T0.6990.83
159_P242_D0.6990.83
76_F135_Q0.6940.82
58_M123_F0.6930.82
194_P197_W0.6930.82
160_H314_L0.6900.82
57_S131_T0.6880.82
266_T320_P0.6880.82
56_E109_E0.6870.81
41_V86_A0.6860.81
162_I284_R0.6860.81
14_L258_Q0.6790.81
107_R121_E0.6780.81
256_L331_I0.6740.80
76_F80_Q0.6700.80
177_R180_S0.6690.80
267_E317_K0.6680.80
20_M94_I0.6650.79
129_Q171_M0.6640.79
296_I305_R0.6610.79
39_D42_K0.6590.79
78_A81_K0.6580.79
329_S332_E0.6560.78
162_I287_A0.6550.78
72_A224_H0.6550.78
54_I111_C0.6550.78
158_V238_V0.6540.78
152_H203_Q0.6540.78
320_P323_H0.6500.78
307_L314_L0.6500.78
140_G186_F0.6490.78
66_C73_A0.6480.78
118_R121_E0.6440.77
217_I223_L0.6440.77
23_V362_V0.6440.77
54_I129_Q0.6440.77
321_S324_K0.6430.77
88_T93_E0.6430.77
167_A321_S0.6420.77
131_T186_F0.6410.77
336_R339_V0.6410.77
207_L212_S0.6410.77
219_S223_L0.6400.77
312_L335_H0.6390.77
60_E237_E0.6390.77
178_F181_S0.6380.76
73_A329_S0.6330.76
316_E320_P0.6320.76
287_A296_I0.6310.76
162_I166_A0.6280.75
135_Q244_P0.6260.75
129_Q185_I0.6250.75
36_M90_H0.6250.75
105_W108_Q0.6240.75
164_L338_V0.6230.75
208_F212_S0.6220.75
148_I152_H0.6210.75
14_L72_A0.6200.74
308_T341_G0.6190.74
12_F142_A0.6180.74
115_R119_T0.6170.74
310_D313_R0.6160.74
248_S251_V0.6150.74
357_S362_V0.6150.74
334_L337_Q0.6130.74
353_A356_G0.6130.74
322_A327_A0.6120.73
16_I63_T0.6090.73
129_Q224_H0.6090.73
296_I310_D0.6090.73
221_K231_P0.6080.73
74_G81_K0.6070.73
167_A259_A0.6070.73
20_M63_T0.6070.73
102_F359_I0.6060.73
23_V93_E0.6060.73
171_M181_S0.6050.73
184_N288_C0.6040.72
202_Q205_E0.6030.72
85_Q354_D0.6010.72
70_N330_A0.6000.72
99_T181_S0.6000.72
180_S183_P0.6000.72
350_D363_K0.5980.72
60_E136_H0.5970.72
56_E111_C0.5970.72
63_T138_H0.5970.72
342_L346_Q0.5940.71
186_F192_N0.5930.71
137_V258_Q0.5920.71
206_A209_R0.5920.71
331_I335_H0.5920.71
16_I134_G0.5920.71
136_H235_T0.5900.71
138_H228_R0.5900.71
33_D90_H0.5850.70
105_W124_G0.5850.70
355_G361_L0.5850.70
270_F273_Q0.5840.70
127_I189_F0.5830.70
224_H285_F0.5830.70
139_V257_I0.5820.70
137_V238_V0.5820.70
96_G244_P0.5810.70
327_A331_I0.5810.70
148_I211_L0.5800.69
314_L321_S0.5800.69
342_L345_A0.5800.69
58_M133_F0.5800.69
169_P180_S0.5790.69
183_P288_C0.5790.69
151_L155_S0.5780.69
19_E239_R0.5770.69
356_G360_G0.5770.69
39_D363_K0.5760.69
251_V254_A0.5750.69
259_A331_I0.5730.69
56_E62_A0.5730.69
333_A337_Q0.5730.69
76_F250_A0.5730.69
329_S333_A0.5730.69
58_M132_V0.5720.68
327_A330_A0.5720.68
151_L227_I0.5700.68
337_Q343_N0.5690.68
196_P277_Y0.5690.68
222_D226_D0.5680.68
144_G231_P0.5670.68
155_S194_P0.5640.67
152_H197_W0.5630.67
60_E116_Y0.5610.67
66_C72_A0.5610.67
55_T110_V0.5600.67
318_I331_I0.5600.67
119_T123_F0.5590.67
136_H140_G0.5590.67
149_Y267_E0.5590.67
24_N31_S0.5580.67
41_V85_Q0.5580.67
351_F360_G0.5580.67
98_G260_T0.5540.66
115_R193_G0.5540.66
20_M83_V0.5530.66
136_H239_R0.5530.66
178_F285_F0.5520.66
89_D312_L0.5520.66
287_A291_G0.5510.66
136_H245_L0.5510.66
29_D107_R0.5500.65
54_I186_F0.5490.65
115_R239_R0.5490.65
110_V117_Q0.5470.65
71_Q74_G0.5470.65
53_D136_H0.5440.65
22_V87_A0.5440.65
99_T185_I0.5430.64
213_Y302_G0.5430.64
14_L69_I0.5430.64
67_R174_T0.5420.64
351_F361_L0.5420.64
69_I258_Q0.5400.64
59_L81_K0.5390.64
124_G177_R0.5390.64
277_Y280_Y0.5390.64
69_I260_T0.5380.64
160_H310_D0.5360.63
352_V361_L0.5360.63
224_H229_P0.5350.63
194_P281_K0.5350.63
130_A189_F0.5340.63
175_D289_R0.5340.63
289_R293_E0.5340.63
352_V357_S0.5340.63
198_V203_Q0.5330.63
43_N70_N0.5310.63
348_M361_L0.5310.63
287_A294_G0.5310.63
57_S130_A0.5300.63
84_L87_A0.5290.62
111_C138_H0.5290.62
353_A357_S0.5260.62
255_G326_G0.5240.62
332_E340_S0.5240.62
209_R212_S0.5240.62
37_L90_H0.5230.62
104_K172_Q0.5230.62
55_T130_A0.5230.62
331_I334_L0.5210.61
145_D353_A0.5200.61
51_K113_N0.5200.61
132_V185_I0.5200.61
80_Q84_L0.5180.61
100_H181_S0.5170.61
323_H332_E0.5170.61
100_H296_I0.5170.61
21_Q95_C0.5150.60
39_D292_L0.5140.60
221_K224_H0.5130.60
293_E341_G0.5120.60
53_D60_E0.5110.60
244_P250_A0.5110.60
136_H243_T0.5110.60
58_M62_A0.5080.59
125_Y128_Q0.5080.59
141_C229_P0.5080.59
143_S147_A0.5070.59
162_I180_S0.5060.59
143_S312_L0.5060.59
22_V31_S0.5050.59
341_G346_Q0.5050.59
261_A265_L0.5050.59
183_P186_F0.5040.59
111_C116_Y0.5030.59
227_I233_F0.5020.58
292_L338_V0.5020.58
54_I110_V0.5020.58
61_L79_M0.5020.58
28_Y362_V0.5010.58
65_V72_A0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r8gA 2 0.9651 100 0.17 Contact Map
2gwdA 2 0.9597 100 0.221 Contact Map
3nztA 1 0.9624 100 0.371 Contact Map
1va6A 1 0.9543 100 0.394 Contact Map
3ln6A 2 0.9435 100 0.406 Contact Map
3ln7A 2 0.9462 100 0.414 Contact Map
3ig5A 1 0.9973 100 0.469 Contact Map
4hppA 3 0.7366 98.8 0.878 Contact Map
4baxA 5 0.5565 98.4 0.895 Contact Map
4is4A 4 0.5027 98.4 0.895 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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