GREMLIN Database
PRET - NAD-dependent dihydropyrimidine dehydrogenase subunit PreT
UniProt: P76440 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14074
Length: 412 (371)
Sequences: 7252 (4339)
Seq/√Len: 225.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
316_A328_R4.4791.00
151_T192_K3.9661.00
230_H319_G3.8721.00
55_N58_G3.8451.00
257_L337_A3.5251.00
123_T148_Y3.3801.00
376_F405_Y3.1311.00
182_A185_E3.1041.00
226_P316_A2.9211.00
214_L378_A2.9131.00
214_L402_I2.8311.00
149_D190_T2.8291.00
155_K196_E2.7501.00
255_S278_Q2.7341.00
139_A214_L2.7191.00
255_S336_A2.7081.00
255_S338_D2.7021.00
159_P193_C2.6931.00
288_L302_R2.6821.00
318_E328_R2.5231.00
328_R334_T2.4921.00
153_Y210_N2.4801.00
318_E326_H2.4791.00
326_H334_T2.4581.00
329_L333_L2.3821.00
178_D182_A2.3521.00
164_R178_D2.3441.00
50_S62_T2.3131.00
357_E361_N2.3091.00
152_I191_I2.2411.00
148_Y403_H2.2181.00
207_K374_Q2.1721.00
320_N323_T2.1591.00
126_K149_D2.1091.00
318_E334_T2.1091.00
281_T309_I2.0971.00
125_G211_R2.0961.00
60_A109_E2.0811.00
182_A186_K2.0721.00
365_T369_Q2.0571.00
57_K61_E2.0441.00
231_S323_T2.0301.00
51_I101_L2.0041.00
374_Q405_Y2.0031.00
403_H407_E1.9821.00
283_V335_M1.9701.00
108_F112_T1.9241.00
239_F272_L1.9171.00
217_V381_I1.9161.00
254_Q278_Q1.9091.00
135_A214_L1.8791.00
324_F340_I1.8581.00
196_E199_N1.8521.00
224_G228_F1.8451.00
144_T189_V1.8091.00
192_K195_N1.7801.00
132_A159_P1.7751.00
122_K144_T1.7691.00
281_T307_S1.7511.00
50_S55_N1.7421.00
132_A154_E1.7281.00
128_A153_Y1.7161.00
383_E394_T1.7131.00
233_V317_V1.6951.00
312_F333_L1.6941.00
320_N325_K1.6821.00
257_L335_M1.6641.00
60_A64_R1.6641.00
130_I215_V1.6421.00
204_E208_A1.6301.00
317_V324_F1.6261.00
151_T190_T1.6231.00
146_Q403_H1.6181.00
49_R53_F1.5771.00
207_K372_D1.5631.00
317_V340_I1.5621.00
43_D46_K1.5571.00
151_T210_N1.5571.00
328_R332_E1.5531.00
158_H178_D1.5431.00
76_V388_V1.5091.00
195_N201_L1.5051.00
157_A194_N1.4951.00
322_V327_V1.4891.00
129_I136_G1.4621.00
205_Q209_E1.4601.00
56_F104_F1.4591.00
372_D375_V1.4441.00
369_Q374_Q1.4361.00
257_L327_V1.4331.00
223_S347_H1.4181.00
159_P191_I1.4051.00
337_A340_I1.3921.00
301_A306_V1.3821.00
159_P181_I1.3731.00
395_G399_A1.3711.00
50_S58_G1.3671.00
287_E290_E1.3671.00
257_L283_V1.3611.00
358_P361_N1.3491.00
129_I150_V1.3481.00
126_K209_E1.3461.00
122_K149_D1.3421.00
299_T303_E1.3321.00
301_A308_I1.3241.00
326_H336_A1.3111.00
309_I312_F1.3111.00
128_A210_N1.3051.00
273_K306_V1.2911.00
269_A282_C1.2851.00
319_G325_K1.2831.00
260_G263_D1.2761.00
301_A305_G1.2731.00
214_L398_A1.2691.00
322_V326_H1.2601.00
256_A277_C1.2561.00
243_A271_T1.2501.00
283_V312_F1.2431.00
195_N200_T1.2431.00
377_A382_V1.2351.00
357_E363_I1.2321.00
103_R107_D1.2271.00
216_T378_A1.2251.00
362_T381_I1.2181.00
266_M282_C1.2181.00
124_L149_D1.2141.00
97_D100_R1.2111.00
369_Q373_P1.2101.00
51_I105_V1.2051.00
318_E325_K1.1961.00
287_E311_G1.1911.00
197_V201_L1.1901.00
352_A355_E1.1901.00
153_Y192_K1.1781.00
68_A141_V1.1751.00
358_P363_I1.1741.00
177_L181_I1.1731.00
202_T205_Q1.1681.00
255_S281_T1.1641.00
58_G61_E1.1621.00
90_A95_P1.1611.00
311_G314_P1.1601.00
82_L88_T1.1581.00
393_K397_E1.1541.00
370_T377_A1.1511.00
75_R107_D1.1441.00
222_G235_I1.1411.00
258_I269_A1.1361.00
351_D355_E1.1351.00
325_K334_T1.1341.00
46_K62_T1.1311.00
181_I185_E1.1271.00
291_F295_E1.1241.00
283_V329_L1.1201.00
305_G308_I1.1191.00
295_E299_T1.1151.00
256_A272_L1.0941.00
309_I333_L1.0941.00
130_I206_L1.0871.00
315_V328_R1.0861.00
48_I101_L1.0861.00
146_Q400_E1.0661.00
401_A404_H1.0661.00
135_A378_A1.0601.00
158_H165_N1.0561.00
198_G201_L1.0541.00
126_K151_T1.0521.00
386_K390_Y1.0461.00
322_V325_K1.0451.00
329_L335_M1.0441.00
69_L152_I1.0421.00
165_N169_Q1.0411.00
156_E241_Q1.0391.00
185_E191_I1.0361.00
302_R308_I1.0281.00
369_Q405_Y1.0281.00
212_A402_I1.0141.00
251_S339_K1.0131.00
154_E193_C1.0091.00
291_F298_F1.0031.00
239_F341_I0.9991.00
57_K114_M0.9901.00
203_L206_L0.9851.00
198_G202_T0.9841.00
269_A280_V0.9821.00
62_T65_E0.9791.00
364_K382_V0.9771.00
228_F342_L0.9721.00
127_V143_L0.9641.00
364_K368_Y0.9581.00
83_C87_C0.9491.00
215_V381_I0.9491.00
57_K112_T0.9451.00
312_F329_L0.9441.00
164_R177_L0.9421.00
132_A191_I0.9361.00
49_R52_Y0.9361.00
148_Y407_E0.9351.00
351_D354_A0.9351.00
82_L87_C0.9331.00
358_P362_T0.9321.00
302_R305_G0.9311.00
125_G406_L0.9261.00
163_L388_V0.9251.00
166_G169_Q0.9241.00
68_A142_T0.9231.00
252_I339_K0.9211.00
84_Q87_C0.9191.00
43_D62_T0.9191.00
84_Q88_T0.9191.00
264_V267_D0.9181.00
234_E339_K0.9170.99
127_V402_I0.9150.99
51_I56_F0.9140.99
101_L105_V0.9140.99
357_E360_R0.9130.99
94_A97_D0.9090.99
281_T337_A0.9080.99
225_L228_F0.9070.99
108_F111_Q0.9050.99
382_V394_T0.9010.99
143_L402_I0.8960.99
128_A213_V0.8950.99
294_S297_E0.8940.99
254_Q338_D0.8920.99
129_I214_L0.8910.99
109_E113_G0.8810.99
315_V332_E0.8800.99
289_D292_P0.8780.99
301_A304_L0.8770.99
383_E390_Y0.8690.99
137_L152_I0.8680.99
156_E220_S0.8670.99
132_A193_C0.8660.99
204_E372_D0.8640.99
42_T66_N0.8630.99
125_G148_Y0.8630.99
99_G102_Q0.8590.99
63_I67_N0.8560.99
364_K377_A0.8550.99
290_E293_A0.8520.99
369_Q376_F0.8520.99
239_F275_L0.8510.99
227_L230_H0.8510.99
350_L354_A0.8510.99
362_T377_A0.8500.99
141_V145_N0.8420.99
367_N401_A0.8410.99
93_D97_D0.8400.99
99_G103_R0.8400.99
205_Q208_A0.8380.99
289_D293_A0.8380.99
124_L148_Y0.8370.99
184_I187_M0.8350.99
293_A297_E0.8340.99
291_F297_E0.8280.99
126_K210_N0.8270.99
225_L314_P0.8250.99
183_R187_M0.8240.99
139_A398_A0.8230.99
213_V375_V0.8220.99
40_A393_K0.8210.99
98_I101_L0.8200.99
394_T397_E0.8170.99
97_D101_L0.8140.99
158_H164_R0.8140.99
43_D65_E0.8120.99
102_Q106_T0.8090.99
61_E65_E0.7960.99
67_N73_C0.7930.99
368_Y382_V0.7920.99
235_I238_D0.7850.98
178_D185_E0.7800.98
136_G152_I0.7780.98
234_E242_R0.7760.98
203_L207_K0.7750.98
294_S298_F0.7750.98
50_S53_F0.7750.98
266_M301_A0.7750.98
154_E191_I0.7730.98
326_H329_L0.7720.98
378_A398_A0.7670.98
101_L106_T0.7640.98
327_V340_I0.7620.98
123_T407_E0.7600.98
139_A378_A0.7600.98
91_G94_A0.7590.98
368_Y397_E0.7580.98
184_I189_V0.7560.98
169_Q174_Q0.7550.98
315_V318_E0.7550.98
378_A402_I0.7540.98
100_R103_R0.7510.98
281_T335_M0.7490.98
201_L205_Q0.7480.98
142_T396_K0.7470.98
97_D103_R0.7470.98
94_A98_I0.7470.98
144_T187_M0.7460.98
227_L319_G0.7420.98
288_L295_E0.7410.98
110_Q113_G0.7370.98
253_P339_K0.7360.98
319_G322_V0.7350.98
142_T145_N0.7320.98
197_V200_T0.7310.98
284_A287_E0.7290.97
104_F108_F0.7280.97
119_P122_K0.7280.97
116_I186_K0.7270.97
285_R311_G0.7260.97
98_I102_Q0.7220.97
47_F102_Q0.7220.97
212_A405_Y0.7200.97
364_K369_Q0.7190.97
100_R104_F0.7160.97
274_V304_L0.7160.97
368_Y394_T0.7150.97
84_Q89_R0.7120.97
137_L181_I0.7090.97
70_G74_A0.7080.97
169_Q175_S0.7070.97
291_F294_S0.7070.97
109_E114_M0.7070.97
204_E207_K0.7060.97
402_I406_L0.7020.97
139_A216_T0.7020.97
369_Q401_A0.7020.97
33_P88_T0.7020.97
356_L360_R0.7010.97
243_A275_L0.7000.97
238_D241_Q0.6980.97
300_S304_L0.6970.97
304_L308_I0.6960.97
98_I103_R0.6950.97
228_F317_V0.6900.96
195_N202_T0.6890.96
261_G291_F0.6880.96
157_A165_N0.6870.96
400_E404_H0.6850.96
89_R93_D0.6830.96
89_R92_V0.6780.96
46_K49_R0.6780.96
212_A406_L0.6770.96
289_D294_S0.6760.96
175_S178_D0.6750.96
129_I140_S0.6740.96
179_A182_A0.6720.96
266_M270_S0.6720.96
300_S305_G0.6710.96
224_G227_L0.6700.96
350_L355_E0.6680.96
140_S152_I0.6660.96
153_Y209_E0.6660.96
97_D106_T0.6650.96
362_T365_T0.6640.96
268_V271_T0.6620.96
103_R106_T0.6610.96
163_L172_L0.6590.95
387_T390_Y0.6590.95
40_A392_V0.6580.95
33_P89_R0.6570.95
220_S349_R0.6560.95
99_G106_T0.6560.95
299_T302_R0.6550.95
110_Q179_A0.6540.95
299_T305_G0.6500.95
183_R186_K0.6490.95
127_V148_Y0.6490.95
219_L381_I0.6490.95
302_R307_S0.6480.95
37_A86_G0.6480.95
267_D274_V0.6480.95
95_P99_G0.6480.95
289_D297_E0.6460.95
230_H317_V0.6450.95
99_G105_V0.6440.95
229_E319_G0.6440.95
94_A101_L0.6420.95
169_Q173_P0.6400.95
236_A240_L0.6390.95
284_A292_P0.6380.95
58_G62_T0.6380.95
41_Q393_K0.6370.95
42_T67_N0.6340.94
43_D66_N0.6340.94
278_Q307_S0.6340.94
237_V241_Q0.6310.94
267_D271_T0.6280.94
356_L362_T0.6270.94
76_V172_L0.6260.94
99_G104_F0.6260.94
136_G216_T0.6250.94
95_P98_I0.6250.94
165_N168_P0.6240.94
233_V324_F0.6240.94
126_K211_R0.6220.94
99_G107_D0.6210.94
377_A381_I0.6140.93
152_I189_V0.6130.93
236_A268_V0.6120.93
369_Q372_D0.6110.93
148_Y211_R0.6110.93
350_L353_F0.6100.93
35_S43_D0.6090.93
128_A209_E0.6070.93
202_T207_K0.6070.93
128_A206_L0.6060.93
250_I341_I0.6050.93
221_S349_R0.6040.93
185_E193_C0.6030.93
145_N187_M0.6020.93
357_E362_T0.6010.93
298_F302_R0.6010.93
35_S46_K0.6000.93
127_V406_L0.5980.93
255_S277_C0.5970.92
167_I172_L0.5970.92
317_V342_L0.5960.92
169_Q172_L0.5960.92
87_C90_A0.5950.92
264_V268_V0.5940.92
137_L184_I0.5940.92
64_R67_N0.5930.92
198_G204_E0.5910.92
104_F107_D0.5880.92
290_E294_S0.5880.92
82_L98_I0.5880.92
212_A374_Q0.5880.92
66_N396_K0.5870.92
217_V352_A0.5870.92
291_F296_K0.5840.92
300_S308_I0.5840.92
129_I216_T0.5830.92
79_T99_G0.5830.92
378_A394_T0.5820.91
302_R306_V0.5810.91
321_K325_K0.5810.91
174_Q177_L0.5800.91
42_T63_I0.5800.91
310_D313_T0.5790.91
44_P49_R0.5790.91
102_Q105_V0.5780.91
376_F401_A0.5780.91
324_F327_V0.5780.91
325_K328_R0.5760.91
370_T375_V0.5750.91
97_D102_Q0.5750.91
309_I335_M0.5750.91
196_E200_T0.5740.91
64_R109_E0.5740.91
242_R248_G0.5730.91
98_I104_F0.5720.91
93_D101_L0.5710.91
285_R314_P0.5710.91
156_E165_N0.5710.91
268_V343_A0.5690.91
117_Y145_N0.5690.91
176_V180_E0.5670.90
120_G146_Q0.5670.90
259_I340_I0.5660.90
56_F108_F0.5650.90
290_E297_E0.5640.90
219_L348_A0.5630.90
94_A99_G0.5630.90
181_I184_I0.5620.90
234_E341_I0.5620.90
170_F173_P0.5620.90
92_V99_G0.5610.90
159_P178_D0.5600.90
265_A284_A0.5580.90
70_G73_C0.5570.90
127_V212_A0.5570.90
101_L104_F0.5570.90
93_D100_R0.5570.90
368_Y401_A0.5570.90
292_P298_F0.5560.90
292_P297_E0.5550.89
278_Q281_T0.5540.89
180_E184_I0.5540.89
171_R174_Q0.5540.89
288_L310_D0.5530.89
399_A403_H0.5510.89
206_L213_V0.5510.89
142_T400_E0.5500.89
295_E298_F0.5490.89
234_E238_D0.5490.89
258_I280_V0.5470.89
270_S304_L0.5470.89
119_P144_T0.5460.89
299_T308_I0.5450.89
35_S41_Q0.5450.89
95_P101_L0.5440.89
266_M269_A0.5440.89
398_A402_I0.5440.89
285_R313_T0.5430.88
297_E301_A0.5430.88
83_C86_G0.5430.88
93_D98_I0.5400.88
236_A343_A0.5400.88
134_P388_V0.5400.88
322_V329_L0.5390.88
235_I342_L0.5380.88
82_L90_A0.5380.88
318_E321_K0.5370.88
231_S319_G0.5340.88
284_A311_G0.5340.88
92_V101_L0.5330.88
79_T82_L0.5330.88
221_S347_H0.5330.88
267_D275_L0.5330.88
300_S303_E0.5320.87
371_R377_A0.5320.87
32_A35_S0.5280.87
207_K373_P0.5270.87
88_T92_V0.5260.87
286_E311_G0.5250.87
268_V341_I0.5250.87
116_I120_G0.5250.87
233_V342_L0.5230.87
82_L86_G0.5220.86
94_A103_R0.5220.86
143_L399_A0.5210.86
143_L148_Y0.5200.86
254_Q339_K0.5200.86
102_Q107_D0.5190.86
181_I193_C0.5190.86
378_A395_G0.5170.86
178_D181_I0.5170.86
92_V97_D0.5170.86
93_D99_G0.5160.86
40_A389_V0.5150.86
74_A77_C0.5150.86
124_L211_R0.5150.86
57_K115_E0.5140.86
64_R183_R0.5140.86
190_T210_N0.5140.86
92_V102_Q0.5130.85
61_E64_R0.5130.85
243_A250_I0.5120.85
63_I70_G0.5110.85
283_V314_P0.5110.85
239_F243_A0.5090.85
138_Q395_G0.5090.85
304_L307_S0.5090.85
67_N70_G0.5090.85
83_C89_R0.5090.85
226_P229_E0.5060.85
278_Q338_D0.5050.85
359_Q363_I0.5050.85
202_T206_L0.5040.84
129_I398_A0.5020.84
177_L184_I0.5020.84
397_E400_E0.5000.84
197_V205_Q0.5000.84
326_H337_A0.5000.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1gteA 4 1 100 0.174 Contact Map
4ylfB 1 1 100 0.183 Contact Map
2vdcG 1 0.9879 100 0.2 Contact Map
1cjcA 1 0.7015 100 0.534 Contact Map
1mo9A 2 0.7767 100 0.543 Contact Map
1lqtA 1 0.699 100 0.55 Contact Map
1o94A 2 0.9102 100 0.554 Contact Map
2q0lA 2 0.6675 100 0.558 Contact Map
3lzwA 2 0.6869 100 0.563 Contact Map
3k30A 2 0.9029 100 0.567 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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