GREMLIN Database
TAM - Trans-aconitate 2-methyltransferase
UniProt: P76145 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13812
Length: 252 (214)
Sequences: 12024 (9865)
Seq/√Len: 674.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_Y57_R3.4651.00
57_R79_Q2.9431.00
115_H118_S2.8991.00
120_L124_G2.7531.00
117_V174_C2.7111.00
65_P82_E2.7071.00
36_A94_L2.6931.00
59_T94_L2.6931.00
71_A80_F2.6191.00
95_D121_N2.5421.00
108_D111_E2.5271.00
68_I80_F2.4921.00
68_I82_E2.4711.00
90_P94_L2.4141.00
65_P69_A2.3991.00
97_I116_L2.3431.00
39_G102_S2.3391.00
46_T58_I2.3151.00
61_I83_A2.2881.00
40_C60_G2.2791.00
116_L126_L2.1561.00
70_E73_S2.1481.00
97_I120_L2.1321.00
40_C46_T2.0231.00
115_H119_L2.0161.00
34_Y59_T1.9911.00
99_A102_S1.9631.00
62_D68_I1.9051.00
59_T81_V1.8761.00
83_A89_Q1.7931.00
30_E33_E1.7741.00
38_L85_I1.7631.00
34_Y121_N1.7211.00
120_L126_L1.6991.00
210_T213_E1.6481.00
85_I106_L1.6411.00
68_I72_R1.6271.00
38_L116_L1.5801.00
110_Y171_E1.5671.00
166_Y170_S1.5621.00
47_A75_L1.5511.00
35_V96_L1.5241.00
33_E56_A1.5211.00
34_Y94_L1.5131.00
102_S106_L1.5111.00
39_G99_A1.4851.00
47_A74_A1.4461.00
48_L51_Q1.4261.00
66_A69_A1.4191.00
88_W115_H1.4181.00
46_T60_G1.4141.00
111_E114_P1.4121.00
110_Y114_P1.4011.00
60_G80_F1.3971.00
112_L116_L1.3931.00
189_S192_A1.3871.00
69_A72_R1.3771.00
59_T79_Q1.3761.00
120_L174_C1.3551.00
223_Q227_E1.3541.00
63_S84_D1.3401.00
91_V118_S1.3311.00
60_G71_A1.3241.00
47_A51_Q1.3241.00
50_Q77_D1.3231.00
161_G164_A1.3121.00
84_D87_N1.2961.00
106_L112_L1.2811.00
191_Q222_H1.2801.00
33_E96_L1.2551.00
45_S98_F1.2531.00
167_D171_E1.2451.00
40_C71_A1.2371.00
22_E25_A1.2351.00
37_D60_G1.2151.00
35_V49_L1.2131.00
226_E229_Y1.1961.00
69_A73_S1.1961.00
116_L120_L1.1941.00
188_P192_A1.1901.00
141_L144_E1.1791.00
214_Q217_F1.1741.00
85_I102_S1.1631.00
81_V90_P1.1611.00
219_K222_H1.1601.00
215_Q219_K1.1531.00
100_N129_Q1.1481.00
196_W200_T1.1421.00
88_W119_L1.1351.00
97_I119_L1.1341.00
62_D67_M1.1321.00
36_A97_I1.1271.00
21_V48_L1.0941.00
218_L222_H1.0881.00
25_A52_R1.0811.00
162_V166_Y1.0781.00
45_S100_N1.0471.00
110_Y168_I1.0451.00
101_A131_P1.0441.00
16_R19_P1.0311.00
103_L106_L1.0301.00
213_E217_F1.0281.00
220_R223_Q1.0201.00
136_E140_V1.0131.00
140_V143_R1.0061.00
40_C62_D1.0021.00
139_H143_R1.0011.00
103_L128_V0.9961.00
86_R106_L0.9931.00
224_M227_E0.9661.00
49_L98_F0.9661.00
136_E139_H0.9641.00
219_K223_Q0.9611.00
223_Q226_E0.9591.00
21_V25_A0.9581.00
210_T214_Q0.9561.00
37_D58_I0.9501.00
33_E95_D0.9361.00
42_P67_M0.9321.00
91_V115_H0.9271.00
22_E26_R0.9261.00
50_Q78_C0.9251.00
142_M145_V0.9231.00
64_S67_M0.9181.00
34_Y95_D0.9171.00
167_D170_S0.9151.00
89_Q115_H0.9101.00
224_M228_Q0.9101.00
113_F169_L0.9071.00
193_I196_W0.9041.00
194_I222_H0.9031.00
20_A45_S0.9011.00
90_P93_A0.9001.00
48_L52_R0.8991.00
222_H226_E0.8951.00
37_D61_I0.8881.00
83_A88_W0.8841.00
211_E214_Q0.8801.00
215_Q218_L0.8691.00
38_L112_L0.8671.00
59_T90_P0.8571.00
32_V56_A0.8531.00
33_E125_V0.8521.00
34_Y93_A0.8521.00
93_A119_L0.8481.00
37_D98_F0.8471.00
216_L220_R0.8471.00
20_A23_L0.8421.00
143_R146_A0.8421.00
198_T218_L0.8341.00
32_V55_A0.8311.00
36_A119_L0.8301.00
101_A104_Q0.8261.00
214_Q218_L0.8261.00
103_L130_M0.8241.00
136_E142_M0.8181.00
12_F15_E0.8171.00
142_M146_A0.8151.00
21_V52_R0.8131.00
138_T141_L0.8121.00
141_L145_V0.8101.00
94_L119_L0.8091.00
114_P172_A0.8061.00
183_Y186_Q0.8051.00
86_R107_P0.8031.00
90_P119_L0.8021.00
196_W199_A0.7881.00
23_L127_A0.7871.00
46_T80_F0.7841.00
61_I81_V0.7841.00
35_V55_A0.7811.00
158_P164_A0.7801.00
27_V127_A0.7751.00
32_V35_V0.7731.00
212_S216_L0.7701.00
93_A121_N0.7691.00
177_D181_T0.7661.00
103_L113_F0.7661.00
73_S76_P0.7641.00
86_R111_E0.7631.00
220_R224_M0.7621.00
191_Q195_D0.7531.00
203_R206_L0.7511.00
137_P141_L0.7471.00
33_E121_N0.7471.00
75_L78_C0.7471.00
63_S82_E0.7361.00
137_P143_R0.7351.00
213_E216_L0.7321.00
177_D180_R0.7231.00
96_L125_V0.7231.00
158_P162_V0.7221.00
160_A164_A0.7211.00
109_H168_I0.7201.00
192_A195_D0.7141.00
16_R20_A0.7141.00
126_L169_L0.7111.00
144_E224_M0.7071.00
164_A167_D0.7061.00
91_V119_L0.7061.00
85_I112_L0.7051.00
18_R21_V0.7051.00
61_I88_W0.7011.00
137_P144_E0.7001.00
87_N111_E0.6991.00
137_P146_A0.6951.00
75_L80_F0.6901.00
18_R22_E0.6881.00
111_E115_H0.6861.00
136_E145_V0.6831.00
72_R79_Q0.6831.00
117_V172_A0.6821.00
45_S129_Q0.6791.00
29_L33_E0.6751.00
35_V58_I0.6721.00
12_F195_D0.6711.00
130_M165_Y0.6661.00
36_A61_I0.6641.00
39_G106_L0.6611.00
195_D199_A0.6591.00
226_E230_P0.6591.00
39_G63_S0.6541.00
19_P23_L0.6531.00
201_G204_P0.6521.00
46_T78_C0.6511.00
113_F172_A0.6501.00
160_A165_Y0.6471.00
110_Y172_A0.6451.00
103_L112_L0.6431.00
216_L219_K0.6431.00
211_E215_Q0.6431.00
87_N115_H0.6411.00
192_A196_W0.6381.00
45_S48_L0.6331.00
24_L27_V0.6311.00
34_Y92_Q0.6281.00
100_N104_Q0.6271.00
13_S16_R0.6271.00
105_W131_P0.6251.00
40_C61_I0.6251.00
142_M147_W0.6211.00
139_H142_M0.6191.00
51_Q55_A0.6181.00
202_L205_W0.6171.00
65_P72_R0.6171.00
12_F199_A0.6161.00
40_C68_I0.6151.00
209_L212_S0.6091.00
157_E160_A0.6051.00
104_Q130_M0.6031.00
195_D198_T0.6031.00
26_R29_L0.6011.00
38_L88_W0.5921.00
218_L221_Y0.5911.00
44_N47_A0.5901.00
28_P52_R0.5901.00
100_N105_W0.5891.00
138_T142_M0.5891.00
203_R207_Q0.5871.00
138_T146_A0.5861.00
176_V179_W0.5851.00
20_A24_L0.5851.00
104_Q131_P0.5841.00
140_V144_E0.5841.00
156_R159_L0.5841.00
190_H193_I0.5811.00
52_R56_A0.5781.00
182_T185_H0.5761.00
86_R108_D0.5741.00
35_V98_F0.5711.00
117_V173_G0.5671.00
137_P140_V0.5661.00
197_V200_T0.5651.00
162_V165_Y0.5641.00
62_D82_E0.5631.00
137_P145_V0.5621.00
143_R147_W0.5611.00
93_A118_S0.5601.00
209_L217_F0.5581.00
163_H166_Y0.5571.00
221_Y225_L0.5561.00
96_L127_A0.5551.00
16_R100_N0.5541.00
101_A129_Q0.5541.00
184_Y187_M0.5521.00
68_I71_A0.5521.00
47_A50_Q0.5481.00
131_P135_L0.5471.00
101_A105_W0.5461.00
204_P208_D0.5451.00
125_V128_V0.5451.00
23_L26_R0.5421.00
217_F221_Y0.5421.00
164_A168_I0.5411.00
12_F16_R0.5401.00
202_L206_L0.5381.00
13_S17_S0.5371.00
40_C80_F0.5371.00
42_P100_N0.5361.00
206_L209_L0.5351.00
183_Y187_M0.5321.00
36_A90_P0.5311.00
139_H144_E0.5311.00
113_F117_V0.5301.00
60_G78_C0.5291.00
206_L210_T0.5261.00
14_A17_S0.5240.99
44_N48_L0.5190.99
70_E74_A0.5190.99
194_I198_T0.5190.99
139_H146_A0.5180.99
209_L213_E0.5180.99
139_H145_V0.5170.99
86_R112_L0.5150.99
102_S105_W0.5140.99
168_I171_E0.5120.99
19_P24_L0.5090.99
29_L32_V0.5010.99
56_A59_T0.5010.99
89_Q119_L0.5000.99
66_A70_E0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2p35A 2 0.9643 100 0.277 Contact Map
3ccfA 1 0.9365 100 0.337 Contact Map
2yqzA 1 0.9722 100 0.397 Contact Map
1vl5A 1 0.8889 100 0.409 Contact Map
3g5tA 1 0.9802 100 0.412 Contact Map
3egeA 1 0.9127 100 0.426 Contact Map
4hg2A 1 0.9008 100 0.438 Contact Map
3ujcA 1 0.9127 100 0.438 Contact Map
4ineA 2 0.9365 100 0.439 Contact Map
4kriA 1 0.9365 100 0.443 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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