ABGB - ECOLI - GREMLIN Contacts

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ABGB - p-aminobenzoyl-glutamate hydrolase subunit B

UniProt:	P76052	Sequences: MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLASALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTPGENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13351
Length:	481 (362)
Sequences:	2601 (1979)
Seq/√Len:	104.0

ECOLI - ABGB

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
69_F	140_F	3.690	1.00
213_A	287_T	3.590	1.00
198_A	207_G	3.422	1.00
43_L	82_Y	3.411	1.00
25_Q	38_W	3.174	1.00
338_T	343_Q	2.850	1.00
193_F	217_M	2.754	1.00
242_G	253_A	2.744	1.00
68_S	139_R	2.738	1.00
24_D	371_N	2.700	1.00
214_V	240_N	2.673	1.00
93_G	97_P	2.663	1.00
210_A	250_Q	2.658	1.00
113_L	172_P	2.658	1.00
232_A	263_E	2.609	1.00
192_R	290_E	2.607	1.00
113_L	369_L	2.553	1.00
18_R	123_I	2.511	1.00
25_Q	42_H	2.433	1.00
26_I	42_H	2.378	1.00
43_L	115_G	2.376	1.00
48_E	56_R	2.334	1.00
16_R	20_T	2.330	1.00
126_K	129_L	2.317	1.00
75_V	137_T	2.299	1.00
227_H	273_R	2.298	1.00
77_A	164_V	2.298	1.00
46_A	49_S	2.274	1.00
215_T	218_T	2.265	1.00
161_F	341_D	2.244	1.00
11_A	127_K	2.241	1.00
194_K	288_T	2.214	1.00
153_T	157_R	2.161	1.00
77_A	167_A	2.141	1.00
174_A	362_L	2.140	1.00
44_A	56_R	2.100	1.00
221_T	274_V	2.055	1.00
273_R	276_K	2.047	1.00
19_Y	120_A	2.037	1.00
38_W	41_E	2.005	1.00
28_D	101_T	1.994	1.00
275_A	279_E	1.982	1.00
192_R	254_E	1.939	1.00
282_A	289_V	1.927	1.00
37_F	63_N	1.903	1.00
276_K	286_E	1.874	1.00
279_E	283_L	1.851	1.00
239_T	256_L	1.837	1.00
187_I	267_V	1.825	1.00
16_R	375_P	1.797	1.00
167_A	341_D	1.789	1.00
194_K	290_E	1.780	1.00
212_D	223_F	1.773	1.00
190_S	292_R	1.769	1.00
7_F	131_Q	1.734	1.00
274_V	291_C	1.721	1.00
259_I	293_F	1.718	1.00
315_S	319_T	1.716	1.00
89_S	103_G	1.703	1.00
227_H	286_E	1.693	1.00
72_G	135_G	1.680	1.00
208_R	287_T	1.665	1.00
117_A	377_A	1.641	1.00
117_A	121_A	1.640	1.00
139_R	163_D	1.626	1.00
11_A	128_W	1.625	1.00
19_Y	116_T	1.612	1.00
271_Y	293_F	1.606	1.00
82_Y	115_G	1.600	1.00
271_Y	275_A	1.596	1.00
220_G	281_A	1.593	1.00
311_Y	315_S	1.583	1.00
192_R	252_Q	1.571	1.00
41_E	45_S	1.568	1.00
12_I	124_A	1.560	1.00
205_H	229_I	1.551	1.00
190_S	254_E	1.550	1.00
77_A	141_Y	1.546	1.00
18_R	46_A	1.538	1.00
271_Y	295_K	1.528	1.00
10_D	14_A	1.528	1.00
16_R	372_E	1.520	1.00
141_Y	167_A	1.509	1.00
194_K	252_Q	1.504	1.00
32_T	86_A	1.495	1.00
190_S	256_L	1.473	1.00
48_E	54_V	1.450	1.00
92_A	97_P	1.449	1.00
169_T	345_S	1.440	1.00
216_L	219_T	1.431	1.00
268_Q	272_D	1.424	1.00
91_Q	366_E	1.422	1.00
41_E	56_R	1.419	1.00
52_F	67_A	1.397	1.00
278_A	289_V	1.397	1.00
78_L	118_A	1.394	1.00
18_R	22_I	1.393	1.00
44_A	54_V	1.385	1.00
117_A	170_W	1.385	1.00
11_A	15_D	1.358	0.99
46_A	50_A	1.352	0.99
228_I	233_R	1.346	0.99
217_M	282_A	1.343	0.99
170_W	348_N	1.339	0.99
22_I	26_I	1.338	0.99
94_C	97_P	1.332	0.99
360_F	364_H	1.324	0.99
25_Q	29_H	1.312	0.99
22_I	42_H	1.311	0.99
18_R	21_D	1.308	0.99
29_H	38_W	1.298	0.99
17_Q	21_D	1.294	0.99
44_A	48_E	1.292	0.99
264_M	339_S	1.288	0.99
278_A	291_C	1.288	0.99
194_K	251_A	1.285	0.99
52_F	126_K	1.284	0.99
35_E	105_N	1.282	0.99
205_H	230_E	1.270	0.99
215_T	219_T	1.260	0.99
276_K	283_L	1.258	0.99
11_A	14_A	1.253	0.99
92_A	96_Q	1.250	0.99
280_G	283_L	1.244	0.99
38_W	42_H	1.241	0.99
214_V	255_V	1.239	0.99
218_T	240_N	1.235	0.99
272_D	276_K	1.223	0.99
307_E	344_N	1.221	0.99
52_F	69_F	1.216	0.99
168_L	306_L	1.211	0.99
216_L	285_T	1.206	0.99
162_D	340_N	1.203	0.99
274_V	278_A	1.203	0.99
180_N	323_N	1.185	0.99
177_G	180_N	1.183	0.99
26_I	115_G	1.179	0.99
217_M	278_A	1.163	0.98
55_T	68_S	1.150	0.98
189_A	237_A	1.148	0.98
92_A	368_V	1.140	0.98
53_T	68_S	1.126	0.98
32_T	105_N	1.126	0.98
36_E	63_N	1.121	0.98
275_A	291_C	1.120	0.98
214_V	253_A	1.119	0.98
54_V	67_A	1.117	0.98
22_I	46_A	1.113	0.98
122_A	140_F	1.105	0.98
216_L	220_G	1.100	0.98
45_S	49_S	1.097	0.98
352_T	356_N	1.095	0.98
167_A	337_L	1.092	0.98
240_N	253_A	1.088	0.98
150_S	297_C	1.086	0.98
180_N	321_E	1.083	0.97
311_Y	324_S	1.082	0.97
66_I	160_V	1.081	0.97
7_F	10_D	1.078	0.97
233_R	270_I	1.076	0.97
46_A	119_F	1.070	0.97
70_G	73_K	1.068	0.97
167_A	343_Q	1.066	0.97
154_F	157_R	1.066	0.97
113_L	374_A	1.064	0.97
52_F	140_F	1.060	0.97
155_M	158_E	1.053	0.97
68_S	137_T	1.049	0.97
141_Y	160_V	1.048	0.97
191_W	291_C	1.046	0.97
155_M	337_L	1.042	0.97
118_A	140_F	1.042	0.97
34_F	145_G	1.041	0.97
166_A	306_L	1.036	0.97
268_Q	295_K	1.033	0.97
44_A	65_F	1.032	0.97
45_S	48_E	1.030	0.97
352_T	358_K	1.028	0.97
154_F	158_E	1.028	0.97
303_N	306_L	1.027	0.97
76_I	168_L	1.027	0.97
40_A	63_N	1.022	0.96
211_L	240_N	1.015	0.96
229_I	326_E	1.010	0.96
269_H	273_R	1.008	0.96
42_H	46_A	1.003	0.96
350_A	373_V	1.001	0.96
7_F	11_A	1.001	0.96
183_T	300_Y	0.995	0.96
174_A	350_A	0.995	0.96
13_E	16_R	0.994	0.96
191_W	278_A	0.986	0.96
75_V	139_R	0.984	0.96
178_M	376_Y	0.979	0.95
74_P	165_D	0.977	0.95
191_W	255_V	0.969	0.95
94_C	368_V	0.966	0.95
29_H	101_T	0.964	0.95
139_R	161_F	0.963	0.95
96_Q	368_V	0.961	0.95
90_Q	96_Q	0.961	0.95
297_C	336_T	0.958	0.95
210_A	249_V	0.950	0.95
313_A	316_H	0.942	0.94
188_Q	246_P	0.939	0.94
15_D	19_Y	0.937	0.94
185_A	261_A	0.936	0.94
208_R	213_A	0.930	0.94
143_C	333_I	0.926	0.94
352_T	370_A	0.918	0.94
209_S	226_E	0.910	0.93
40_A	82_Y	0.910	0.93
161_F	164_V	0.909	0.93
352_T	357_G	0.909	0.93
139_R	164_V	0.908	0.93
40_A	44_A	0.907	0.93
144_P	155_M	0.906	0.93
304_R	308_N	0.905	0.93
19_Y	374_A	0.905	0.93
328_A	347_N	0.904	0.93
362_L	367_T	0.904	0.93
264_M	267_V	0.903	0.93
221_T	237_A	0.902	0.93
86_A	245_S	0.899	0.93
125_V	138_V	0.897	0.93
275_A	289_V	0.895	0.93
43_L	65_F	0.895	0.93
75_V	165_D	0.894	0.93
300_Y	335_A	0.892	0.93
89_S	104_E	0.885	0.92
185_A	299_S	0.881	0.92
186_N	331_K	0.880	0.92
360_F	366_E	0.876	0.92
329_F	333_I	0.873	0.92
309_A	313_A	0.873	0.92
156_V	161_F	0.866	0.91
78_L	121_A	0.865	0.91
190_S	294_D	0.865	0.91
172_P	350_A	0.860	0.91
15_D	124_A	0.857	0.91
277_I	281_A	0.852	0.91
184_L	329_F	0.851	0.91
229_I	232_A	0.850	0.91
308_N	312_Q	0.850	0.91
128_W	131_Q	0.848	0.91
212_D	218_T	0.848	0.91
200_A	238_I	0.848	0.91
37_F	41_E	0.847	0.91
227_H	276_K	0.847	0.91
189_A	257_Y	0.846	0.90
235_H	259_I	0.840	0.90
183_T	347_N	0.836	0.90
46_A	123_I	0.835	0.90
178_M	372_E	0.834	0.90
55_T	158_E	0.829	0.90
191_W	275_A	0.826	0.89
222_N	225_N	0.822	0.89
212_D	215_T	0.820	0.89
356_N	372_E	0.819	0.89
191_W	217_M	0.818	0.89
12_I	375_P	0.816	0.89
193_F	213_A	0.816	0.89
69_F	126_K	0.815	0.89
15_D	127_K	0.814	0.89
50_A	123_I	0.814	0.89
217_M	221_T	0.813	0.89
310_M	346_L	0.813	0.89
312_Q	315_S	0.812	0.89
310_M	344_N	0.810	0.88
54_V	69_F	0.805	0.88
76_I	166_A	0.803	0.88
224_L	277_I	0.802	0.88
124_A	128_W	0.802	0.88
44_A	58_V	0.801	0.88
298_S	336_T	0.800	0.88
193_F	278_A	0.798	0.88
213_A	285_T	0.796	0.88
367_T	371_N	0.796	0.88
80_G	140_F	0.794	0.87
139_R	160_V	0.794	0.87
241_S	254_E	0.792	0.87
48_E	53_T	0.791	0.87
42_H	45_S	0.788	0.87
280_G	284_M	0.788	0.87
318_G	321_E	0.787	0.87
25_Q	28_D	0.787	0.87
278_A	282_A	0.786	0.87
224_L	270_I	0.786	0.87
74_P	134_Q	0.781	0.87
189_A	274_V	0.781	0.87
332_Q	345_S	0.781	0.87
348_N	377_A	0.780	0.87
33_R	36_E	0.775	0.86
211_L	242_G	0.773	0.86
314_L	322_W	0.773	0.86
96_Q	371_N	0.773	0.86
32_T	35_E	0.772	0.86
193_F	282_A	0.771	0.86
197_A	207_G	0.769	0.86
301_L	304_R	0.767	0.86
352_T	355_E	0.765	0.85
177_G	351_A	0.765	0.85
192_R	292_R	0.764	0.85
22_I	116_T	0.761	0.85
173_E	183_T	0.759	0.85
197_A	248_V	0.759	0.85
54_V	65_F	0.759	0.85
40_A	65_F	0.759	0.85
90_Q	106_G	0.757	0.85
264_M	268_Q	0.755	0.85
80_G	118_A	0.752	0.84
94_C	98_T	0.751	0.84
95_A	98_T	0.748	0.84
160_V	163_D	0.747	0.84
190_S	246_P	0.745	0.84
197_A	210_A	0.745	0.84
345_S	349_I	0.745	0.84
173_E	347_N	0.744	0.84
78_L	125_V	0.740	0.84
13_E	375_P	0.735	0.83
217_M	277_I	0.733	0.83
25_Q	33_R	0.723	0.82
337_L	345_S	0.723	0.82
269_H	272_D	0.720	0.82
261_A	270_I	0.720	0.82
164_V	341_D	0.717	0.82
237_A	240_N	0.717	0.82
306_L	309_A	0.714	0.81
33_R	105_N	0.714	0.81
8_I	124_A	0.714	0.81
102_P	105_N	0.712	0.81
235_H	274_V	0.707	0.81
187_I	293_F	0.706	0.81
228_I	270_I	0.703	0.80
259_I	271_Y	0.702	0.80
172_P	362_L	0.701	0.80
208_R	286_E	0.700	0.80
90_Q	368_V	0.700	0.80
47_L	67_A	0.700	0.80
279_E	289_V	0.696	0.80
21_D	25_Q	0.696	0.80
155_M	160_V	0.695	0.80
258_L	331_K	0.695	0.80
78_L	168_L	0.694	0.79
22_I	119_F	0.693	0.79
11_A	124_A	0.693	0.79
117_A	348_N	0.691	0.79
196_I	208_R	0.689	0.79
203_S	206_L	0.686	0.79
207_G	249_V	0.686	0.79
47_L	142_G	0.683	0.78
8_I	12_I	0.680	0.78
356_N	359_V	0.679	0.78
6_R	10_D	0.679	0.78
188_Q	256_L	0.678	0.78
54_V	57_N	0.676	0.78
156_V	164_V	0.674	0.77
72_G	136_G	0.673	0.77
308_N	311_Y	0.673	0.77
67_A	126_K	0.672	0.77
141_Y	337_L	0.669	0.77
233_R	236_Y	0.667	0.77
113_L	117_A	0.667	0.77
33_R	148_G	0.666	0.77
193_F	214_V	0.665	0.76
350_A	369_L	0.662	0.76
64_A	155_M	0.659	0.76
58_V	158_E	0.658	0.76
221_T	235_H	0.657	0.76
352_T	359_V	0.657	0.76
55_T	160_V	0.656	0.76
14_A	17_Q	0.656	0.76
281_A	284_M	0.655	0.75
235_H	277_I	0.655	0.75
232_A	266_D	0.655	0.75
212_D	222_N	0.652	0.75
71_Q	135_G	0.652	0.75
77_A	337_L	0.650	0.75
212_D	219_T	0.648	0.75
44_A	57_N	0.648	0.75
203_S	328_A	0.644	0.74
235_H	238_I	0.641	0.74
351_A	359_V	0.639	0.74
29_H	42_H	0.638	0.73
276_K	279_E	0.633	0.73
15_D	18_R	0.631	0.73
66_I	155_M	0.629	0.72
353_G	359_V	0.628	0.72
197_A	250_Q	0.628	0.72
200_A	236_Y	0.627	0.72
245_S	248_V	0.626	0.72
185_A	331_K	0.626	0.72
73_K	135_G	0.625	0.72
261_A	266_D	0.625	0.72
212_D	225_N	0.620	0.71
185_A	267_V	0.619	0.71
126_K	130_E	0.619	0.71
90_Q	93_G	0.618	0.71
267_V	295_K	0.617	0.71
261_A	267_V	0.617	0.71
178_M	373_V	0.616	0.71
172_P	373_V	0.616	0.71
62_P	154_F	0.615	0.71
14_A	127_K	0.612	0.70
123_I	127_K	0.609	0.70
333_I	336_T	0.608	0.70
270_I	274_V	0.605	0.70
91_Q	98_T	0.603	0.69
186_N	246_P	0.602	0.69
361_A	364_H	0.602	0.69
268_Q	271_Y	0.602	0.69
90_Q	94_C	0.601	0.69
188_Q	294_D	0.601	0.69
228_I	269_H	0.601	0.69
215_T	255_V	0.600	0.69
236_Y	239_T	0.598	0.69
272_D	275_A	0.597	0.68
173_E	362_L	0.595	0.68
82_Y	142_G	0.594	0.68
66_I	139_R	0.593	0.68
228_I	266_D	0.593	0.68
173_E	203_S	0.593	0.68
279_E	288_T	0.592	0.68
55_T	58_V	0.591	0.68
262_P	299_S	0.590	0.68
67_A	140_F	0.589	0.67
150_S	154_F	0.587	0.67
172_P	369_L	0.587	0.67
262_P	327_L	0.586	0.67
181_T	376_Y	0.586	0.67
170_W	346_L	0.586	0.67
58_V	155_M	0.585	0.67
72_G	137_T	0.582	0.67
176_A	179_F	0.580	0.66
306_L	338_T	0.578	0.66
54_V	126_K	0.576	0.66
9_D	12_I	0.574	0.65
185_A	266_D	0.573	0.65
15_D	120_A	0.573	0.65
227_H	269_H	0.573	0.65
203_S	330_A	0.572	0.65
328_A	331_K	0.571	0.65
336_T	339_S	0.569	0.65
70_G	136_G	0.569	0.65
63_N	144_P	0.568	0.65
156_V	160_V	0.568	0.65
251_A	365_R	0.567	0.65
306_L	344_N	0.567	0.65
17_Q	49_S	0.564	0.64
22_I	120_A	0.563	0.64
10_D	13_E	0.562	0.64
12_I	120_A	0.561	0.64
273_R	286_E	0.560	0.64
46_A	120_A	0.559	0.63
42_H	67_A	0.559	0.63
91_Q	94_C	0.558	0.63
113_L	350_A	0.558	0.63
84_A	115_G	0.558	0.63
34_F	150_S	0.558	0.63
309_A	312_Q	0.555	0.63
212_D	216_L	0.555	0.63
189_A	291_C	0.554	0.63
82_Y	155_M	0.552	0.62
201_A	329_F	0.552	0.62
15_D	123_I	0.551	0.62
55_T	139_R	0.550	0.62
213_A	216_L	0.549	0.62
31_E	39_S	0.548	0.62
40_A	58_V	0.546	0.62
119_F	123_I	0.545	0.62
282_A	287_T	0.544	0.61
332_Q	335_A	0.543	0.61
50_A	126_K	0.542	0.61
111_H	171_H	0.542	0.61
91_Q	252_Q	0.541	0.61
48_E	65_F	0.541	0.61
197_A	365_R	0.540	0.61
22_I	25_Q	0.540	0.61
168_L	343_Q	0.539	0.61
216_L	281_A	0.537	0.60
23_A	112_N	0.537	0.60
26_I	46_A	0.536	0.60
85_L	88_L	0.536	0.60
184_L	330_A	0.535	0.60
214_V	218_T	0.535	0.60
69_F	129_L	0.534	0.60
76_I	138_V	0.534	0.60
211_L	218_T	0.534	0.60
169_T	337_L	0.533	0.60
68_S	163_D	0.533	0.60
229_I	262_P	0.532	0.60
173_E	330_A	0.531	0.60
213_A	282_A	0.531	0.60
181_T	314_L	0.530	0.59
92_A	366_E	0.530	0.59
33_R	149_G	0.529	0.59
187_I	261_A	0.529	0.59
9_D	13_E	0.528	0.59
355_E	359_V	0.528	0.59
139_R	158_E	0.527	0.59
20_T	24_D	0.527	0.59
74_P	135_G	0.526	0.59
79_L	143_C	0.524	0.59
214_V	219_T	0.523	0.58
369_L	373_V	0.523	0.58
74_P	136_G	0.522	0.58
235_H	239_T	0.521	0.58
29_H	102_P	0.521	0.58
8_I	11_A	0.521	0.58
241_S	256_L	0.521	0.58
49_S	67_A	0.519	0.58
277_I	280_G	0.519	0.58
199_H	247_N	0.519	0.58
12_I	15_D	0.519	0.58
43_L	118_A	0.519	0.58
109_C	112_N	0.516	0.57
240_N	255_V	0.516	0.57
58_V	62_P	0.516	0.57
74_P	137_T	0.515	0.57
337_L	340_N	0.515	0.57
222_N	236_Y	0.512	0.57
201_A	346_L	0.512	0.57
191_W	214_V	0.512	0.57
184_L	262_P	0.511	0.57
263_E	266_D	0.511	0.57
236_Y	240_N	0.511	0.57
298_S	339_S	0.509	0.56
88_L	364_H	0.508	0.56
48_E	52_F	0.506	0.56
31_E	111_H	0.505	0.56
71_Q	131_Q	0.505	0.56
80_G	115_G	0.505	0.56
215_T	240_N	0.505	0.56
276_K	280_G	0.504	0.56
259_I	270_I	0.504	0.56
266_D	269_H	0.503	0.55
207_G	210_A	0.502	0.55
305_T	308_N	0.502	0.55
72_G	130_E	0.502	0.55
33_R	63_N	0.502	0.55
193_F	210_A	0.501	0.55
352_T	360_F	0.501	0.55
88_L	108_G	0.500	0.55

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3ramA	4	0.8004	100	0.579	Contact Map
1xmbA	1	0.7464	100	0.613	Contact Map
3io1A	4	0.7235	100	0.614	Contact Map
4ewtA	4	0.7755	100	0.624	Contact Map
1ysjA	2	0.7297	100	0.632	Contact Map
3pfoA	2	0.7838	100	0.649	Contact Map
3rzaA	2	0.736	100	0.663	Contact Map
3gb0A	2	0.736	100	0.665	Contact Map
3dljA	2	0.7859	100	0.666	Contact Map
1cg2A	4	0.7755	100	0.671	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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