GREMLIN Database
PUUD - Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD
UniProt: P76038 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13909
Length: 254 (222)
Sequences: 5995 (3990)
Seq/√Len: 267.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
11_G62_L3.3621.00
170_L239_G3.0131.00
107_R214_P2.9311.00
101_N105_E2.8631.00
167_L207_A2.7461.00
9_V43_I2.6671.00
9_V41_L2.6341.00
7_N41_L2.4651.00
43_I62_L2.4621.00
163_E168_S2.2991.00
168_S175_S2.2581.00
100_I110_I2.2491.00
195_R211_I2.2191.00
196_V199_R2.1311.00
120_L216_A2.1111.00
100_I119_E2.0691.00
158_E176_N2.0511.00
148_P153_Y2.0431.00
98_A101_N1.9991.00
148_P151_Q1.9871.00
166_L169_A1.9281.00
10_I64_G1.9071.00
133_C145_P1.8941.00
165_G197_E1.8801.00
43_I61_K1.8771.00
110_I120_L1.8561.00
65_I103_A1.8421.00
163_E175_S1.8111.00
35_I40_G1.8031.00
160_Q176_N1.7651.00
215_F239_G1.7611.00
156_S178_W1.7601.00
158_E178_W1.7051.00
194_L208_V1.6571.00
38_A245_Q1.6531.00
6_N38_A1.6431.00
14_M44_A1.6041.00
93_D115_R1.5771.00
197_E209_S1.5661.00
131_K145_P1.5581.00
100_I216_A1.5451.00
149_V153_Y1.5431.00
7_N39_G1.5241.00
67_L100_I1.5101.00
207_A221_W1.5061.00
143_E146_E1.5051.00
124_T194_L1.5011.00
151_Q154_A1.4841.00
27_L44_A1.4651.00
93_D119_E1.4601.00
239_G243_A1.4551.00
162_E199_R1.4501.00
162_E195_R1.4401.00
112_A120_L1.4211.00
97_M123_A1.4171.00
149_V154_A1.4111.00
102_A106_R1.4111.00
11_G45_L1.3991.00
81_E188_K1.3981.00
190_V196_V1.3751.00
200_S203_G1.3661.00
34_A40_G1.3521.00
51_E54_L1.3481.00
66_Y113_I1.3461.00
30_K34_A1.3421.00
100_I123_A1.3421.00
167_L197_E1.3411.00
60_P106_R1.3361.00
124_T128_L1.3201.00
64_G244_C1.3191.00
148_P154_A1.3131.00
198_A207_A1.3121.00
129_H188_K1.3121.00
62_L65_I1.3071.00
166_L217_L1.3001.00
164_G168_S1.2871.00
210_V213_H1.2851.00
57_Q60_P1.2841.00
133_C144_D1.2761.00
242_T246_H1.2511.00
10_I244_C1.2471.00
107_R213_H1.2421.00
159_V221_W1.2151.00
239_G242_T1.2111.00
50_A54_L1.2101.00
161_V198_A1.2091.00
103_A108_I1.1991.00
165_G195_R1.1961.00
29_E33_N1.1931.00
120_L218_G1.1901.00
67_L110_I1.1891.00
210_V216_A1.1791.00
136_P139_L1.1751.00
161_V167_L1.1731.00
56_E102_A1.1731.00
9_V62_L1.1581.00
160_Q175_S1.1571.00
186_G204_L1.1301.00
119_E122_V1.1301.00
31_Y34_A1.1241.00
161_V177_F1.1231.00
196_V205_V1.1221.00
56_E106_R1.1221.00
51_E55_L1.1191.00
100_I120_L1.0991.00
191_S194_L1.0981.00
107_R212_N1.0941.00
117_L187_A1.0931.00
9_V61_K1.0901.00
121_V208_V1.0811.00
162_E165_G1.0811.00
150_E153_Y1.0791.00
121_V147_L1.0751.00
238_E242_T1.0711.00
121_V187_A1.0671.00
117_L208_V1.0661.00
165_G209_S1.0651.00
104_L124_T1.0621.00
65_I108_I1.0471.00
162_E190_V1.0451.00
140_E143_E1.0401.00
121_V191_S1.0341.00
6_N245_Q1.0201.00
170_L217_L1.0181.00
37_H242_T1.0161.00
217_L236_L1.0121.00
66_Y240_F1.0091.00
102_A105_E1.0081.00
55_L98_A1.0041.00
124_T210_V1.0041.00
148_P152_Q0.9931.00
161_V168_S0.9891.00
135_Q140_E0.9861.00
111_F240_F0.9851.00
52_P98_A0.9841.00
240_F244_C0.9831.00
31_Y35_I0.9761.00
167_L217_L0.9741.00
117_L218_G0.9651.00
158_E201_P0.9481.00
215_F240_F0.9241.00
234_R238_E0.9231.00
215_F243_A0.9231.00
124_T192_P0.9231.00
166_L209_S0.9161.00
131_K184_G0.9161.00
30_K35_I0.9151.00
149_V152_Q0.9141.00
226_N234_R0.9081.00
121_V194_L0.9041.00
115_R139_L0.8911.00
92_R96_S0.8901.00
40_G241_I0.8781.00
199_R203_G0.8691.00
200_S204_L0.8611.00
18_R46_P0.8551.00
137_E146_E0.8531.00
127_S188_K0.8481.00
150_E154_A0.8471.00
65_I99_I0.8471.00
197_E207_A0.8441.00
59_L103_A0.8421.00
93_D139_L0.8340.99
64_G240_F0.8290.99
121_V126_G0.8260.99
242_T245_Q0.8240.99
156_S226_N0.8220.99
243_A246_H0.8160.99
219_V236_L0.8090.99
157_H181_S0.8030.99
12_V26_T0.8000.99
59_L108_I0.7970.99
129_H135_Q0.7930.99
159_V198_A0.7910.99
13_V68_P0.7910.99
110_I216_A0.7820.99
33_N36_I0.7810.99
101_N127_S0.7740.99
160_Q199_R0.7730.99
32_L36_I0.7730.99
124_T191_S0.7710.99
30_K225_W0.7710.99
140_E144_D0.7710.99
196_V208_V0.7700.99
121_V190_V0.7650.99
110_I119_E0.7620.99
32_L35_I0.7550.99
30_K36_I0.7500.99
12_V68_P0.7490.99
10_I40_G0.7450.99
50_A58_L0.7390.99
43_I58_L0.7360.99
52_P55_L0.7330.99
104_L216_A0.7320.99
24_T28_Q0.7310.99
170_L215_F0.7280.99
187_A208_V0.7270.99
159_V179_V0.7230.99
131_K134_E0.7190.98
160_Q201_P0.7190.98
59_L62_L0.7190.98
100_I124_T0.7190.98
37_H234_R0.7160.98
13_V96_S0.7160.98
195_R209_S0.7140.98
167_L219_V0.7100.98
108_I247_H0.7090.98
120_L208_V0.7040.98
161_V165_G0.7030.98
208_V218_G0.7030.98
155_P226_N0.7020.98
32_L37_H0.7010.98
54_L57_Q0.7010.98
187_A205_V0.7000.98
81_E130_R0.6990.98
20_K44_A0.6970.98
207_A219_V0.6950.98
168_S172_P0.6920.98
29_E91_G0.6910.98
103_A110_I0.6810.98
79_Y128_L0.6810.98
129_H192_P0.6760.98
161_V171_L0.6760.98
96_S119_E0.6750.98
213_H216_A0.6720.98
101_N123_A0.6680.97
114_C222_H0.6670.97
25_Q29_E0.6650.97
111_F219_V0.6650.97
65_I110_I0.6650.97
45_L62_L0.6460.97
104_L210_V0.6460.97
104_L123_A0.6450.97
35_I66_Y0.6440.97
189_V205_V0.6430.97
210_V214_P0.6430.97
193_R211_I0.6430.97
55_L59_L0.6400.97
67_L120_L0.6400.97
194_L210_V0.6380.97
55_L102_A0.6370.97
38_A234_R0.6370.97
33_N228_S0.6360.97
68_P92_R0.6340.97
34_A37_H0.6320.97
97_M101_N0.6320.97
235_I238_E0.6290.96
31_Y36_I0.6280.96
121_V127_S0.6280.96
99_I102_A0.6250.96
66_Y96_S0.6250.96
123_A127_S0.6230.96
164_G169_A0.6180.96
29_E32_L0.6160.96
113_I219_V0.6160.96
50_A55_L0.6150.96
93_D122_V0.6140.96
94_L98_A0.6090.96
55_L58_L0.6080.96
13_V99_I0.6070.96
94_L97_M0.6050.96
133_C140_E0.6020.95
135_Q143_E0.6000.95
190_V208_V0.5980.95
26_T69_G0.5980.95
13_V47_H0.5950.95
166_L215_F0.5940.95
20_K27_L0.5920.95
113_I237_F0.5900.95
159_V167_L0.5890.95
17_N20_K0.5880.95
34_A237_F0.5850.95
94_L126_G0.5840.95
9_V63_D0.5810.94
38_A241_I0.5800.94
59_L102_A0.5800.94
115_R119_E0.5790.94
162_E168_S0.5780.94
51_E56_E0.5770.94
181_S206_E0.5720.94
132_L145_P0.5710.94
167_L198_A0.5680.94
15_C25_Q0.5660.94
180_N226_N0.5650.94
34_A227_S0.5640.94
182_L225_W0.5620.93
69_G222_H0.5620.93
12_V27_L0.5610.93
111_F217_L0.5600.93
31_Y37_H0.5600.93
127_S189_V0.5590.93
160_Q203_G0.5590.93
228_S232_L0.5580.93
166_L170_L0.5560.93
132_L144_D0.5560.93
12_V32_L0.5550.93
63_D247_H0.5510.93
244_C247_H0.5450.92
157_H200_S0.5440.92
30_K33_N0.5400.92
159_V177_F0.5390.92
189_V192_P0.5360.92
174_C232_L0.5350.92
56_E60_P0.5340.92
171_L177_F0.5310.91
69_G114_C0.5300.91
81_E93_D0.5290.91
102_A108_I0.5280.91
33_N37_H0.5280.91
21_G26_T0.5250.91
50_A98_A0.5240.91
190_V205_V0.5240.91
59_L106_R0.5220.91
11_G43_I0.5210.91
132_L204_L0.5210.91
14_M25_Q0.5210.91
97_M119_E0.5190.90
159_V207_A0.5170.90
52_P101_N0.5160.90
131_K144_D0.5160.90
54_L98_A0.5130.90
37_H238_E0.5120.90
36_I233_S0.5110.90
57_Q61_K0.5080.90
227_S233_S0.5050.89
23_A29_E0.5040.89
34_A241_I0.5020.89
164_G195_R0.5020.89
161_V175_S0.5010.89
8_P244_C0.5010.89
170_L235_I0.5010.89
20_K46_P0.5000.89
95_L182_L0.5000.89
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3fijA 4 0.8661 100 0.38 Contact Map
4l8fC 3 0.9213 100 0.447 Contact Map
4zdjA 4 0.878 100 0.455 Contact Map
1l9xA 1 0.9055 100 0.458 Contact Map
1s1mA 4 0.8819 100 0.465 Contact Map
3nvaA 2 0.8701 100 0.478 Contact Map
1vcoA 4 0.8661 100 0.482 Contact Map
2v4uA 1 0.8858 100 0.501 Contact Map
2w7tA 1 0.8819 100 0.505 Contact Map
3uowA 2 0.8386 100 0.526 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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