GREMLIN Database
NHAP2 - K(+)/H(+) antiporter NhaP2
UniProt: P76007 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13896
Length: 578 (474)
Sequences: 5269 (3919)
Seq/√Len: 180.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
112_A119_L3.8221.00
338_W382_S3.7871.00
234_I252_C3.7851.00
307_A374_V3.6521.00
415_E419_Y3.4751.00
197_L243_G3.4091.00
385_W389_K3.3271.00
208_L211_Q3.2231.00
189_V193_Q3.1151.00
109_M119_L3.1091.00
109_M113_W3.0641.00
111_A122_G3.0401.00
300_I304_I3.0341.00
207_Y211_Q3.0141.00
346_P368_A3.0031.00
209_L230_G2.9941.00
108_G119_L2.9261.00
211_Q215_R2.9211.00
304_I308_W2.8401.00
118_D121_E2.8221.00
188_I192_L2.7601.00
430_A434_D2.7081.00
416_Q419_Y2.7071.00
193_Q197_L2.6501.00
110_M309_M2.6251.00
109_M112_A2.6001.00
415_E418_V2.5811.00
86_R89_L2.5281.00
112_A122_G2.5161.00
205_G234_I2.5121.00
418_V421_L2.4631.00
441_T446_L2.4511.00
432_L443_I2.4061.00
360_N363_L2.2951.00
203_L255_L2.2841.00
481_K485_Q2.2791.00
128_I350_A2.2561.00
95_L318_V2.1901.00
166_V349_L2.1641.00
446_L450_N2.1351.00
386_A389_K2.1291.00
303_L370_F2.1251.00
374_V378_L2.1211.00
108_G112_A2.1171.00
85_F92_A2.1001.00
444_A448_R2.0971.00
203_L207_Y2.0541.00
446_L449_D2.0461.00
421_L461_L2.0411.00
48_D293_S1.9471.00
447_F450_N1.9291.00
301_P305_L1.9201.00
478_A481_K1.8991.00
441_T444_A1.8951.00
475_D478_A1.8931.00
108_G123_L1.8801.00
190_D193_Q1.8711.00
329_N332_E1.8511.00
414_W419_Y1.8371.00
431_A434_D1.8131.00
106_L313_A1.7941.00
105_G109_M1.7851.00
306_S371_V1.7821.00
159_G250_Y1.7711.00
286_L344_A1.7631.00
435_L439_K1.7161.00
390_A393_V1.7121.00
50_V53_I1.6921.00
454_H457_G1.6851.00
446_L459_T1.6841.00
319_F330_L1.6821.00
104_S130_G1.6681.00
417_F421_L1.6631.00
405_G408_I1.6451.00
196_G200_V1.6311.00
124_L353_P1.6291.00
40_A44_L1.6251.00
150_V332_E1.6221.00
192_L196_G1.6141.00
435_L438_P1.5941.00
464_G468_C1.5921.00
49_G53_I1.5821.00
422_S426_W1.5761.00
134_A158_S1.5561.00
211_Q214_N1.5501.00
204_G207_Y1.5411.00
441_T448_R1.5011.00
406_L409_H1.5001.00
319_F334_V1.5001.00
443_I450_N1.4991.00
194_Q242_E1.4921.00
193_Q243_G1.4891.00
108_G126_G1.4711.00
47_V291_N1.4651.00
104_S168_L1.4651.00
415_E421_L1.4651.00
443_I447_F1.4581.00
238_T243_G1.4581.00
400_P404_V1.4541.00
468_C472_R1.4491.00
443_I449_D1.4481.00
419_Y423_A1.4471.00
102_I317_S1.4431.00
315_P337_S1.4421.00
412_N419_Y1.4391.00
57_N61_A1.4291.00
282_M344_A1.4211.00
132_T344_A1.4201.00
171_T187_F1.4031.00
227_A267_I1.3961.00
91_P153_T1.3941.00
469_V472_R1.3891.00
461_L465_D1.3801.00
111_A117_L1.3791.00
389_K393_V1.3791.00
404_V408_I1.3791.00
415_E420_Q1.3641.00
407_D410_P1.3591.00
453_L459_T1.3581.00
307_A311_F1.3571.00
479_L483_F1.3561.00
402_S406_L1.3561.00
320_A324_P1.3491.00
389_K395_P1.3331.00
161_N340_G1.3311.00
19_S45_A1.3281.00
294_D297_P1.3261.00
455_P465_D1.3261.00
319_F337_S1.3221.00
23_L41_I1.3201.00
201_I238_T1.3081.00
396_P399_R1.3011.00
443_I448_R1.2961.00
133_D161_N1.2721.00
196_G199_I1.2611.00
434_D437_M1.2561.00
444_A449_D1.2551.00
439_K443_I1.2531.00
150_V336_I1.2511.00
173_I353_P1.2511.00
443_I455_P1.2411.00
266_G270_N1.2391.00
70_A235_F1.2351.00
191_I195_F1.2291.00
137_V141_L1.2281.00
303_L307_A1.2261.00
444_A447_F1.2251.00
294_D366_N1.2181.00
442_R450_N1.2181.00
428_V463_E1.2131.00
418_V422_S1.2121.00
296_L300_I1.2091.00
391_K395_P1.2001.00
354_M365_F1.1981.00
233_L237_L1.1971.00
442_R449_D1.1941.00
448_R465_D1.1931.00
133_D157_E1.1901.00
445_A450_N1.1881.00
439_K442_R1.1881.00
401_V404_V1.1851.00
421_L425_K1.1851.00
342_R346_P1.1841.00
455_P471_G1.1841.00
58_Y61_A1.1831.00
109_M114_L1.1771.00
305_L308_W1.1761.00
444_A450_N1.1701.00
443_I446_L1.1651.00
235_F245_G1.1641.00
117_L122_G1.1601.00
201_I241_L1.1591.00
234_I248_A1.1581.00
89_L93_L1.1471.00
422_S425_K1.1471.00
402_S407_D1.1461.00
413_P419_Y1.1451.00
126_G130_G1.1441.00
403_R406_L1.1411.00
329_N333_R1.1391.00
189_V192_L1.1371.00
401_V408_I1.1311.00
242_E249_V1.1311.00
223_Y263_N1.1291.00
367_V371_V1.1261.00
198_G202_G1.1231.00
205_G237_L1.1221.00
59_P62_Y1.1221.00
199_I251_L1.1171.00
125_I368_A1.1171.00
419_Y425_K1.1161.00
388_K393_V1.1131.00
441_T445_A1.1101.00
186_M189_V1.1021.00
387_A392_V1.1001.00
111_A126_G1.0991.00
127_A130_G1.0901.00
316_L320_A1.0891.00
472_R475_D1.0871.00
291_N294_D1.0871.00
429_G435_L1.0821.00
436_H439_K1.0811.00
480_G484_S1.0781.00
421_L424_D1.0741.00
208_L212_M1.0741.00
323_L333_R1.0711.00
228_L274_L1.0701.00
309_M313_A1.0681.00
107_T111_A1.0621.00
157_E340_G1.0521.00
453_L463_E1.0521.00
477_P481_K1.0511.00
112_A123_L1.0501.00
111_A306_S1.0481.00
416_Q421_L1.0481.00
102_I313_A1.0451.00
323_L326_R1.0451.00
106_L308_W1.0441.00
202_G206_G1.0421.00
478_A482_L1.0391.00
207_Y255_L1.0361.00
334_V337_S1.0351.00
319_F333_R1.0311.00
320_A323_L1.0311.00
449_D462_R1.0311.00
259_R262_R1.0291.00
420_Q423_A1.0291.00
404_V409_H1.0260.99
455_P460_R1.0260.99
433_R439_K1.0210.99
132_T137_V1.0180.99
311_F315_P1.0130.99
210_L259_R1.0090.99
200_V204_G1.0090.99
238_T248_A1.0080.99
294_D362_R1.0060.99
104_S127_A1.0060.99
458_S461_L1.0020.99
467_L477_P0.9990.99
433_R438_P0.9990.99
128_I349_L0.9930.99
398_G401_V0.9920.99
328_F333_R0.9910.99
205_G209_L0.9900.99
200_V203_L0.9890.99
442_R447_F0.9880.99
120_I176_I0.9880.99
468_C471_G0.9870.99
73_L131_S0.9830.99
483_F486_S0.9810.99
302_A367_V0.9800.99
135_A279_Q0.9740.99
302_A305_L0.9740.99
226_L230_G0.9730.99
290_V373_L0.9680.99
420_Q425_K0.9680.99
415_E426_W0.9680.99
403_R407_D0.9640.99
51_G54_P0.9630.99
117_L121_E0.9630.99
105_G108_G0.9610.99
74_L253_G0.9610.99
106_L317_S0.9590.99
178_H181_S0.9570.99
402_S405_G0.9560.99
439_K448_R0.9480.99
141_L154_L0.9410.99
456_T459_T0.9390.99
28_S31_G0.9350.99
311_F378_L0.9310.99
129_V371_V0.9280.99
49_G52_G0.9280.99
110_M308_W0.9270.99
82_A86_R0.9210.99
438_P441_T0.9210.99
75_D79_R0.9200.99
118_D122_G0.9140.99
108_G111_A0.9130.99
157_E161_N0.9120.99
432_L455_P0.9120.99
27_S37_I0.9100.99
480_G483_F0.9100.99
458_S471_G0.9090.99
403_R410_P0.9070.99
401_V406_L0.9040.99
113_W116_N0.9030.99
310_I371_V0.9010.99
339_V342_R0.9010.99
451_Q458_S0.9000.99
419_Y422_S0.8990.99
44_L47_V0.8980.99
431_A435_L0.8950.99
401_V409_H0.8900.99
400_P403_R0.8900.99
440_E447_F0.8860.99
471_G476_L0.8840.98
88_A92_A0.8830.98
417_F420_Q0.8830.98
141_L146_L0.8810.98
110_M113_W0.8790.98
184_S188_I0.8780.98
251_L255_L0.8780.98
401_V405_G0.8780.98
460_R463_E0.8730.98
417_F425_K0.8720.98
430_A441_T0.8710.98
420_Q427_C0.8710.98
157_E343_G0.8690.98
415_E422_S0.8680.98
405_G409_H0.8670.98
435_L444_A0.8660.98
419_Y424_D0.8650.98
476_L479_L0.8650.98
417_F423_A0.8640.98
435_L447_F0.8600.98
169_T352_F0.8590.98
430_A433_R0.8550.98
228_L269_Q0.8540.98
120_I172_L0.8540.98
18_T22_L0.8460.98
481_K484_S0.8450.98
127_A172_L0.8440.98
141_L151_G0.8430.98
473_E480_G0.8420.98
377_L381_T0.8410.98
354_M361_A0.8390.98
389_K392_V0.8370.98
333_R337_S0.8360.98
423_A426_W0.8350.98
402_S409_H0.8350.98
452_L470_I0.8330.98
110_M114_L0.8320.98
412_N420_Q0.8280.98
388_K395_P0.8280.98
133_D136_A0.8270.98
432_L435_L0.8260.98
436_H450_N0.8260.98
119_L123_L0.8230.98
423_A463_E0.8230.98
122_G126_G0.8230.98
318_V337_S0.8220.98
429_G433_R0.8210.98
438_P442_R0.8200.98
160_S247_L0.8170.97
166_V244_S0.8130.97
237_L241_L0.8120.97
419_Y426_W0.8110.97
131_S165_A0.8110.97
399_R405_G0.8090.97
412_N418_V0.8080.97
132_T161_N0.8080.97
399_R402_S0.8070.97
80_T85_F0.8050.97
436_H440_E0.8030.97
476_L480_G0.8020.97
20_S23_L0.8010.97
404_V407_D0.8010.97
398_G406_L0.8010.97
426_W430_A0.8000.97
442_R446_L0.8000.97
134_A154_L0.7990.97
342_R375_S0.7950.97
74_L231_G0.7930.97
310_I342_R0.7930.97
465_D474_R0.7920.97
201_I205_G0.7920.97
399_R403_R0.7910.97
432_L457_G0.7910.97
48_D291_N0.7900.97
415_E423_A0.7890.97
214_N217_A0.7890.97
368_A372_V0.7860.97
439_K446_L0.7840.97
431_A438_P0.7840.97
394_V397_V0.7840.97
402_S408_I0.7810.97
183_I188_I0.7810.97
132_T340_G0.7790.97
480_G485_Q0.7790.97
125_I367_V0.7780.97
403_R409_H0.7770.97
133_D340_G0.7760.97
473_E477_P0.7760.97
299_A302_A0.7750.97
384_S388_K0.7750.97
459_T462_R0.7700.96
99_G103_T0.7680.96
42_G46_G0.7670.96
453_L460_R0.7670.96
416_Q422_S0.7660.96
475_D479_L0.7650.96
20_S280_I0.7630.96
397_V400_P0.7610.96
83_S87_V0.7610.96
385_W388_K0.7600.96
403_R408_I0.7600.96
137_V341_L0.7600.96
317_S321_G0.7550.96
157_E318_V0.7540.96
328_F332_E0.7540.96
413_P416_Q0.7540.96
464_G470_I0.7520.96
18_T229_S0.7510.96
396_P401_V0.7510.96
471_G474_R0.7470.96
201_I243_G0.7470.96
461_L464_G0.7470.96
302_A306_S0.7460.96
418_V423_A0.7440.96
433_R446_L0.7430.96
322_L333_R0.7430.96
465_D472_R0.7410.96
120_I123_L0.7390.95
431_A460_R0.7390.95
338_W341_L0.7380.95
457_G464_G0.7370.95
20_S24_S0.7350.95
160_S244_S0.7330.95
210_L256_L0.7330.95
318_V336_I0.7330.95
376_L379_Q0.7310.95
467_L471_G0.7300.95
429_G432_L0.7290.95
88_A153_T0.7290.95
452_L458_S0.7290.95
434_D438_P0.7280.95
451_Q460_R0.7270.95
54_P61_A0.7260.95
418_V426_W0.7260.95
210_L260_P0.7250.95
98_L102_I0.7230.95
9_L13_G0.7220.95
108_G122_G0.7210.95
458_S464_G0.7200.95
433_R445_A0.7190.95
454_H461_L0.7160.95
68_A282_M0.7150.95
435_L443_I0.7140.95
179_H182_N0.7120.94
85_F254_F0.7110.94
476_L483_F0.7070.94
241_L296_L0.7070.94
430_A435_L0.7070.94
413_P426_W0.7070.94
138_F155_E0.7060.94
418_V428_V0.7040.94
150_V335_F0.7040.94
97_T101_L0.7030.94
129_V342_R0.7030.94
462_R466_V0.7020.94
203_L251_L0.7010.94
133_D158_S0.7010.94
104_S165_A0.7010.94
119_L122_G0.6990.94
131_S134_A0.6990.94
204_G208_L0.6960.94
478_A486_S0.6960.94
470_I474_R0.6930.94
50_V54_P0.6930.94
93_L373_L0.6920.94
62_Y66_N0.6920.94
99_G314_R0.6920.94
199_I203_L0.6910.94
132_T343_G0.6900.93
421_L426_W0.6880.93
338_W379_Q0.6870.93
93_L97_T0.6860.93
413_P418_V0.6860.93
438_P443_I0.6840.93
132_T157_E0.6830.93
244_S274_L0.6820.93
227_A256_L0.6810.93
368_A376_L0.6810.93
469_V474_R0.6800.93
107_T126_G0.6800.93
424_D428_V0.6790.93
298_I302_A0.6780.93
415_E425_K0.6780.93
80_T156_I0.6770.93
416_Q426_W0.6730.93
127_A168_L0.6730.93
326_R329_N0.6720.93
91_P328_F0.6720.93
134_A288_L0.6680.92
124_L172_L0.6680.92
304_I374_V0.6680.92
107_T254_F0.6680.92
73_L345_V0.6680.92
16_L24_S0.6670.92
447_F466_V0.6670.92
431_A437_M0.6670.92
213_I230_G0.6630.92
455_P463_E0.6630.92
158_S161_N0.6630.92
417_F427_C0.6620.92
446_L453_L0.6600.92
412_N416_Q0.6600.92
429_G444_A0.6580.92
16_L20_S0.6570.92
471_G475_D0.6540.92
433_R441_T0.6540.92
91_P94_S0.6520.91
394_V398_G0.6510.91
19_S23_L0.6500.91
316_L395_P0.6500.91
440_E478_A0.6490.91
482_L486_S0.6490.91
455_P461_L0.6480.91
256_L267_I0.6470.91
110_M305_L0.6470.91
438_P446_L0.6470.91
209_L226_L0.6460.91
442_R445_A0.6460.91
57_N60_F0.6450.91
244_S343_G0.6450.91
197_L206_G0.6450.91
33_P134_A0.6440.91
463_E466_V0.6430.91
93_L109_M0.6420.91
412_N423_A0.6420.91
121_E176_I0.6400.91
414_W420_Q0.6390.91
453_L457_G0.6380.91
430_A436_H0.6380.91
363_L367_V0.6370.90
459_T464_G0.6370.90
266_G269_Q0.6370.90
131_S346_P0.6360.90
416_Q427_C0.6340.90
80_T134_A0.6340.90
422_S427_C0.6320.90
397_V402_S0.6280.90
144_K384_S0.6280.90
90_G94_S0.6270.90
84_S130_G0.6250.90
102_I106_L0.6240.90
419_Y429_G0.6230.89
136_A376_L0.6210.89
173_I176_I0.6210.89
70_A232_I0.6200.89
479_L486_S0.6200.89
437_M447_F0.6190.89
420_Q424_D0.6190.89
92_A156_I0.6180.89
154_L350_A0.6170.89
125_I364_F0.6170.89
75_D275_A0.6160.89
96_A99_G0.6140.89
442_R448_R0.6130.89
255_L259_R0.6120.89
423_A427_C0.6120.89
74_L77_G0.6110.89
101_L105_G0.6100.88
91_P325_F0.6090.88
393_V397_V0.6080.88
416_Q424_D0.6080.88
356_A360_N0.6040.88
338_W380_G0.6030.88
280_I375_S0.6030.88
162_D244_S0.6010.88
395_P402_S0.6010.88
307_A375_S0.6000.88
396_P409_H0.6000.88
131_S161_N0.5990.88
17_V24_S0.5990.88
85_F156_I0.5970.87
190_D194_Q0.5970.87
154_L339_V0.5970.87
132_T286_L0.5950.87
398_G402_S0.5950.87
14_S23_L0.5920.87
169_T353_P0.5920.87
472_R476_L0.5920.87
232_I236_S0.5920.87
11_I14_S0.5920.87
158_S347_I0.5920.87
79_R368_A0.5910.87
445_A460_R0.5900.87
53_I57_N0.5890.87
423_A428_V0.5890.87
434_D439_K0.5880.87
186_M190_D0.5880.87
454_H459_T0.5870.87
468_C474_R0.5860.86
292_P295_L0.5860.86
215_R219_P0.5860.86
95_L139_S0.5860.86
467_L470_I0.5850.86
187_F191_I0.5850.86
470_I473_E0.5850.86
421_L428_V0.5840.86
244_S349_L0.5830.86
137_V342_R0.5820.86
100_V164_M0.5820.86
148_E151_G0.5810.86
225_L277_L0.5810.86
253_G282_M0.5800.86
459_T467_L0.5800.86
419_Y431_A0.5800.86
236_S240_A0.5790.86
458_S462_R0.5770.86
457_G469_V0.5770.86
157_E245_G0.5760.85
109_M424_D0.5750.85
209_L256_L0.5750.85
382_S385_W0.5740.85
80_T258_N0.5730.85
44_L298_I0.5730.85
174_A178_H0.5730.85
436_H451_Q0.5720.85
158_S198_G0.5710.85
435_L441_T0.5710.85
435_L446_L0.5700.85
330_L334_V0.5680.85
440_E443_I0.5650.84
121_E360_N0.5640.84
94_S321_G0.5640.84
289_L365_F0.5630.84
282_M348_I0.5630.84
406_L410_P0.5630.84
414_W427_C0.5630.84
63_M231_G0.5620.84
38_F280_I0.5610.84
479_L484_S0.5610.84
100_V103_T0.5600.84
458_S466_V0.5590.84
449_D457_G0.5580.84
91_P156_I0.5570.84
184_S189_V0.5570.84
456_T462_R0.5570.84
305_L309_M0.5570.84
411_E419_Y0.5560.83
117_L363_L0.5550.83
71_I228_L0.5540.83
342_R372_V0.5540.83
400_P405_G0.5530.83
397_V403_R0.5520.83
442_R453_L0.5500.83
465_D469_V0.5500.83
75_D272_D0.5490.83
441_T471_G0.5490.83
128_I368_A0.5480.83
459_T473_E0.5470.82
411_E414_W0.5460.82
198_G206_G0.5460.82
473_E479_L0.5460.82
20_S373_L0.5450.82
157_E202_G0.5430.82
165_A169_T0.5430.82
132_T165_A0.5410.82
75_D372_V0.5410.82
460_R464_G0.5390.82
135_A348_I0.5380.81
102_I321_G0.5380.81
70_A234_I0.5370.81
312_F315_P0.5370.81
445_A449_D0.5370.81
270_N274_L0.5360.81
341_L372_V0.5360.81
331_R335_F0.5350.81
431_A449_D0.5350.81
10_F255_L0.5330.81
65_S250_Y0.5320.81
439_K447_F0.5320.81
474_R478_A0.5320.81
395_P405_G0.5320.81
82_A257_G0.5310.81
471_G486_S0.5280.80
420_Q442_R0.5270.80
83_S86_R0.5270.80
388_K392_V0.5260.80
478_A484_S0.5250.80
433_R444_A0.5240.80
213_I261_I0.5240.80
440_E450_N0.5240.80
387_A391_K0.5230.80
412_N417_F0.5230.80
394_V410_P0.5220.79
394_V400_P0.5220.79
77_G249_V0.5210.79
14_S229_S0.5210.79
76_G132_T0.5210.79
157_E163_P0.5200.79
449_D458_S0.5200.79
106_L109_M0.5190.79
21_I225_L0.5180.79
423_A440_E0.5180.79
419_Y428_V0.5180.79
424_D427_C0.5170.79
267_I371_V0.5160.79
433_R467_L0.5140.78
335_F383_L0.5130.78
411_E440_E0.5120.78
39_L286_L0.5120.78
92_A107_T0.5110.78
103_T107_T0.5100.78
23_L37_I0.5100.78
210_L255_L0.5090.78
447_F452_L0.5090.78
29_R116_N0.5090.78
331_R390_A0.5080.78
212_M233_L0.5080.78
440_E446_L0.5060.77
170_I174_A0.5050.77
246_I249_V0.5030.77
250_Y345_V0.5030.77
456_T460_R0.5020.77
398_G409_H0.5020.77
81_Q84_S0.5020.77
464_G483_F0.5020.77
387_A401_V0.5000.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4czbA 2 0.6713 100 0.428 Contact Map
4cz8A 2 0.6661 100 0.436 Contact Map
4bwzA 2 0.6574 100 0.472 Contact Map
1zcdA 1 0.5934 99.9 0.772 Contact Map
2mdfA 1 0.0986 99.3 0.865 Contact Map
3zuxA 1 0.5138 98.2 0.908 Contact Map
2l0eA 1 0.0536 97.7 0.919 Contact Map
4n7wA 2 0.5173 97.6 0.922 Contact Map
2k3cA 1 0.0536 97.3 0.925 Contact Map
2htgA 1 0.0467 97.2 0.927 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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