GREMLIN Database
BLUF - Blue light- and temperature-regulated antirepressor BluF
UniProt: P75990 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13887
Length: 403 (390)
Sequences: 19945 (16075)
Seq/√Len: 814.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
182_P381_F3.8181.00
190_F395_P3.8171.00
4_T8_R3.4341.00
75_V79_C3.3901.00
219_L257_F3.2801.00
218_D247_T3.1311.00
259_L298_A3.0831.00
329_Q333_T2.9991.00
173_H197_N2.9161.00
223_A264_A2.8981.00
296_K323_D2.8751.00
387_A390_K2.8641.00
176_F228_M2.8231.00
273_I324_R2.6731.00
181_D184_S2.6101.00
70_R73_N2.5601.00
155_D203_A2.5381.00
367_P371_M2.5321.00
233_E271_Q2.5041.00
162_D198_E2.4571.00
372_W376_A2.4461.00
353_T379_E2.4291.00
230_H234_L2.2431.00
370_W386_F2.2001.00
208_Q217_A2.0851.00
228_M392_N2.0851.00
220_K224_L2.0661.00
303_A324_R2.0641.00
346_Q354_S2.0401.00
318_S341_R2.0381.00
15_E19_K1.9911.00
165_A169_T1.9901.00
194_V221_S1.9711.00
156_G210_K1.9571.00
224_L227_T1.9431.00
366_T369_E1.9401.00
121_Y124_R1.9301.00
39_I114_T1.8791.00
170_I224_L1.8711.00
370_W381_F1.8641.00
226_F261_E1.8521.00
75_V80_D1.8481.00
342_Q372_W1.8411.00
175_A391_L1.8101.00
224_L228_M1.8051.00
41_L91_G1.8011.00
179_I380_M1.7971.00
228_M232_L1.7711.00
364_V381_F1.7671.00
174_F221_S1.7611.00
41_L110_F1.7511.00
268_V271_Q1.7331.00
238_M301_S1.7191.00
292_I302_V1.7181.00
180_V386_F1.6901.00
324_R360_S1.6891.00
273_I303_A1.6651.00
24_V122_D1.6651.00
336_H342_Q1.6641.00
179_I324_R1.6571.00
261_E265_N1.6571.00
346_Q376_A1.6461.00
223_A261_E1.6431.00
367_P385_L1.6321.00
134_L143_E1.6241.00
370_W374_E1.6191.00
391_L394_I1.6161.00
336_H368_E1.6141.00
19_K23_M1.6071.00
301_S323_D1.6041.00
223_A265_N1.6041.00
229_A267_L1.5961.00
346_Q350_K1.5841.00
56_E59_K1.5761.00
349_I376_A1.5751.00
325_I352_C1.5741.00
171_N198_E1.5731.00
349_I378_I1.5501.00
137_E143_E1.5451.00
232_L392_N1.5251.00
46_H109_V1.5071.00
243_L274_V1.5051.00
262_I300_I1.5041.00
358_Q379_E1.5011.00
243_L254_A1.4991.00
235_G396_S1.4871.00
116_K119_L1.4851.00
384_D389_A1.4831.00
370_W385_L1.4741.00
358_Q380_M1.4721.00
179_I273_I1.4691.00
60_M63_R1.4671.00
304_I322_P1.4631.00
238_M273_I1.4501.00
188_I380_M1.4401.00
329_Q363_G1.4271.00
47_F139_S1.4271.00
179_I189_A1.4241.00
172_D194_V1.4231.00
20_I75_V1.4071.00
112_K144_I1.3951.00
23_M26_I1.3911.00
318_S334_N1.3771.00
63_R66_C1.3641.00
119_L126_L1.3621.00
233_E268_V1.3531.00
336_H372_W1.3521.00
171_N196_K1.3521.00
133_V136_T1.3281.00
60_M102_R1.3261.00
394_I397_I1.3191.00
260_N264_A1.3081.00
259_L294_S1.2981.00
324_R358_Q1.2891.00
39_I87_F1.2851.00
4_T13_D1.2781.00
335_V372_W1.2781.00
361_A378_I1.2761.00
227_T265_N1.2701.00
289_A293_K1.2631.00
174_F220_K1.2621.00
231_A235_G1.2591.00
262_I269_P1.2321.00
371_M374_E1.2221.00
225_A239_I1.2201.00
165_A171_N1.2111.00
215_Y251_E1.2111.00
289_A319_R1.2031.00
257_F261_E1.2011.00
381_F386_F1.1981.00
229_A239_I1.1921.00
371_M375_S1.1911.00
37_T117_F1.1901.00
194_V217_A1.1881.00
352_C359_V1.1871.00
322_P357_I1.1851.00
83_P86_R1.1831.00
97_L100_H1.1741.00
60_M64_A1.1721.00
293_K297_A1.1691.00
126_L150_W1.1531.00
20_I76_E1.1511.00
181_D188_I1.1491.00
194_V220_K1.1481.00
75_V81_Y1.1441.00
57_Q106_L1.1421.00
88_G144_I1.1381.00
88_G92_M1.1331.00
232_L396_S1.1331.00
61_I98_R1.1311.00
338_S341_R1.1311.00
181_D186_R1.1301.00
92_M96_D1.1291.00
273_I301_S1.1281.00
236_D271_Q1.1221.00
8_R19_K1.1191.00
134_L141_Y1.1171.00
185_R374_E1.1111.00
228_M231_A1.1041.00
179_I240_S1.0981.00
335_V345_I1.0981.00
201_P204_I1.0941.00
255_V259_L1.0941.00
182_P370_W1.0931.00
64_A102_R1.0921.00
259_L263_K1.0861.00
47_F50_L1.0861.00
245_P279_S1.0851.00
367_P370_W1.0831.00
14_D62_Y1.0761.00
43_N108_A1.0751.00
223_A227_T1.0741.00
189_A238_M1.0651.00
50_L160_E1.0651.00
133_V137_E1.0641.00
133_V143_E1.0621.00
336_H366_T1.0621.00
274_V300_I1.0591.00
44_G135_A1.0561.00
172_D220_K1.0531.00
45_S61_I1.0501.00
240_S273_I1.0481.00
56_E60_M1.0481.00
173_H391_L1.0461.00
25_S122_D1.0441.00
229_A233_E1.0401.00
285_F320_F1.0351.00
230_H265_N1.0351.00
7_Y18_K1.0331.00
202_S329_Q1.0301.00
98_R108_A1.0281.00
113_G126_L1.0251.00
258_L274_V1.0241.00
390_K394_I1.0231.00
324_R380_M1.0231.00
23_M74_I1.0231.00
211_D215_Y1.0221.00
350_K354_S1.0201.00
258_L295_L1.0181.00
163_S208_Q1.0181.00
230_H233_E1.0181.00
179_I238_M1.0171.00
25_S29_R1.0171.00
118_Q144_I1.0161.00
347_A354_S1.0131.00
255_V291_A1.0121.00
42_F131_T1.0071.00
255_V294_S1.0051.00
159_K207_G1.0011.00
191_E242_N0.9921.00
44_G52_E0.9921.00
226_F272_I0.9891.00
216_T219_L0.9861.00
27_A38_G0.9851.00
23_M27_A0.9771.00
294_S297_A0.9751.00
392_N396_S0.9751.00
168_P216_T0.9681.00
372_W375_S0.9681.00
343_A354_S0.9651.00
225_A241_I0.9621.00
7_Y22_E0.9591.00
95_F142_F0.9581.00
87_F91_G0.9571.00
174_F225_A0.9571.00
160_E164_C0.9561.00
227_T231_A0.9541.00
128_F132_F0.9541.00
284_R287_E0.9531.00
360_S380_M0.9501.00
282_I316_L0.9501.00
98_R102_R0.9491.00
99_L140_T0.9421.00
287_E291_A0.9411.00
230_H266_A0.9391.00
291_A294_S0.9391.00
59_K63_R0.9381.00
256_S294_S0.9351.00
183_L379_E0.9351.00
253_D257_F0.9311.00
38_G115_S0.9311.00
181_D379_E0.9301.00
349_I359_V0.9281.00
336_H369_E0.9241.00
48_F52_E0.9231.00
213_E216_T0.9221.00
45_S108_A0.9221.00
95_F110_F0.9191.00
222_K261_E0.9111.00
69_P73_N0.9041.00
209_R214_I0.9031.00
10_H18_K0.9011.00
190_F237_K0.9011.00
179_I360_S0.9001.00
228_M396_S0.8951.00
165_A168_P0.8901.00
171_N199_D0.8891.00
205_A214_I0.8861.00
147_E151_L0.8841.00
238_M271_Q0.8831.00
19_K22_E0.8811.00
192_A239_I0.8801.00
256_S260_N0.8761.00
158_D162_D0.8751.00
170_I223_A0.8751.00
91_G142_F0.8701.00
170_I220_K0.8701.00
91_G95_F0.8661.00
251_E257_F0.8661.00
68_D93_E0.8621.00
261_E264_A0.8611.00
364_V373_L0.8591.00
164_C213_E0.8571.00
166_L172_D0.8521.00
91_G112_K0.8511.00
349_I353_T0.8491.00
49_Q52_E0.8441.00
88_G142_F0.8441.00
96_D99_L0.8431.00
21_E25_S0.8381.00
46_H139_S0.8381.00
166_L171_N0.8371.00
150_W154_A0.8361.00
301_S358_Q0.8351.00
174_F194_V0.8341.00
174_F192_A0.8321.00
68_D97_L0.8321.00
226_F267_L0.8311.00
120_T124_R0.8311.00
194_V204_I0.8291.00
183_L374_E0.8281.00
107_Q140_T0.8281.00
146_A149_S0.8271.00
290_E294_S0.8241.00
7_Y19_K0.8221.00
129_F149_S0.8221.00
164_C216_T0.8221.00
178_P387_A0.8181.00
22_E26_I0.8151.00
47_F160_E0.8151.00
289_A321_Q0.8141.00
84_A88_G0.8141.00
222_K258_L0.8121.00
257_F260_N0.8111.00
275_E326_K0.8111.00
180_V187_I0.8111.00
133_V152_F0.8081.00
160_E211_D0.8041.00
293_K321_Q0.8041.00
231_A392_N0.8021.00
333_T369_E0.8011.00
49_Q53_G0.8001.00
342_Q346_Q0.7981.00
111_D127_Q0.7971.00
192_A225_A0.7951.00
114_T144_I0.7871.00
90_A93_E0.7861.00
176_F192_A0.7841.00
208_Q214_I0.7841.00
318_S344_I0.7801.00
219_L261_E0.7791.00
219_L223_A0.7791.00
176_F392_N0.7781.00
290_E293_K0.7771.00
267_L272_I0.7761.00
137_E141_Y0.7721.00
82_A86_R0.7711.00
233_E236_D0.7691.00
176_F190_F0.7671.00
364_V370_W0.7661.00
215_Y247_T0.7651.00
252_P287_E0.7641.00
8_R15_E0.7641.00
44_G132_F0.7621.00
175_A197_N0.7611.00
306_H362_M0.7591.00
7_Y15_E0.7581.00
145_P150_W0.7581.00
81_Y87_F0.7561.00
373_L378_I0.7561.00
92_M95_F0.7561.00
226_F265_N0.7561.00
343_A346_Q0.7551.00
160_E207_G0.7541.00
319_R340_P0.7531.00
334_N337_K0.7531.00
350_K353_T0.7531.00
52_E135_A0.7511.00
178_P390_K0.7501.00
254_A258_L0.7491.00
211_D214_I0.7481.00
231_A234_L0.7471.00
67_Q97_L0.7461.00
277_T306_H0.7461.00
361_A373_L0.7461.00
334_N341_R0.7441.00
341_R369_E0.7391.00
89_K93_E0.7381.00
21_E123_D0.7371.00
49_Q132_F0.7371.00
20_I24_V0.7371.00
287_E290_E0.7341.00
185_R370_W0.7301.00
262_I265_N0.7271.00
85_R88_G0.7261.00
48_F58_V0.7241.00
307_F348_I0.7231.00
109_V139_S0.7221.00
11_I52_E0.7211.00
339_G342_Q0.7201.00
145_P149_S0.7191.00
4_T7_Y0.7191.00
163_S172_D0.7181.00
353_T359_V0.7171.00
111_D130_R0.7161.00
200_S203_A0.7141.00
76_E79_C0.7131.00
167_S220_K0.7131.00
138_Q141_Y0.7111.00
104_D165_A0.7111.00
384_D388_K0.7111.00
248_L291_A0.7091.00
112_K142_F0.7051.00
42_F127_Q0.7051.00
332_I335_V0.7051.00
87_F114_T0.7011.00
11_I59_K0.6991.00
332_I361_A0.6951.00
43_N110_F0.6941.00
202_S206_V0.6931.00
115_S119_L0.6921.00
61_I65_I0.6901.00
390_K395_P0.6901.00
64_A101_E0.6891.00
229_A236_D0.6891.00
304_I316_L0.6881.00
285_F288_F0.6871.00
42_F75_V0.6831.00
231_A396_S0.6811.00
126_L145_P0.6811.00
61_I102_R0.6811.00
86_R89_K0.6791.00
57_Q102_R0.6791.00
19_K74_I0.6771.00
185_R367_P0.6751.00
365_A384_D0.6741.00
214_I246_M0.6701.00
285_F289_A0.6691.00
148_D151_L0.6681.00
17_V21_E0.6671.00
91_G110_F0.6671.00
178_P187_I0.6651.00
163_S166_L0.6651.00
237_K271_Q0.6621.00
334_N338_S0.6611.00
373_L381_F0.6611.00
256_S259_L0.6591.00
248_L280_E0.6591.00
52_E58_V0.6581.00
173_H393_G0.6581.00
31_N35_D0.6571.00
36_V80_D0.6561.00
359_V379_E0.6561.00
342_Q376_A0.6541.00
44_G48_F0.6541.00
162_D196_K0.6521.00
318_S338_S0.6521.00
47_F210_K0.6511.00
301_S324_R0.6491.00
151_L155_D0.6481.00
172_D196_K0.6461.00
113_G127_Q0.6431.00
349_I373_L0.6421.00
95_F140_T0.6411.00
229_A271_Q0.6401.00
272_I300_I0.6371.00
30_R80_D0.6361.00
242_N305_D0.6351.00
18_K21_E0.6351.00
255_V298_A0.6351.00
222_K241_I0.6331.00
27_A30_R0.6321.00
278_E308_G0.6311.00
190_F239_I0.6281.00
46_H107_Q0.6281.00
267_L271_Q0.6261.00
148_D152_F0.6221.00
28_N115_S0.6221.00
39_I81_Y0.6201.00
70_R93_E0.6181.00
79_C90_A0.6171.00
159_K162_D0.6161.00
154_A158_D0.6151.00
219_L247_T0.6151.00
182_P374_E0.6151.00
84_A144_I0.6141.00
63_R67_Q0.6131.00
316_L319_R0.6131.00
180_V185_R0.6131.00
327_I378_I0.6131.00
343_A347_A0.6121.00
208_Q212_G0.6121.00
21_E122_D0.6111.00
245_P280_E0.6101.00
189_A240_S0.6101.00
229_A272_I0.6091.00
223_A257_F0.6091.00
191_E326_K0.6051.00
81_Y86_R0.6031.00
292_I321_Q0.6021.00
325_I357_I0.6021.00
219_L260_N0.6011.00
219_L251_E0.5971.00
239_I267_L0.5961.00
309_A331_L0.5931.00
258_L261_E0.5931.00
175_A389_A0.5911.00
163_S213_E0.5911.00
294_S298_A0.5911.00
113_G125_A0.5891.00
348_I352_C0.5871.00
70_R90_A0.5851.00
95_F99_L0.5841.00
365_A369_E0.5831.00
309_A330_E0.5831.00
109_V134_L0.5831.00
31_N115_S0.5821.00
41_L112_K0.5811.00
163_S217_A0.5811.00
277_T362_M0.5801.00
352_C357_I0.5781.00
193_I242_N0.5781.00
84_A117_F0.5771.00
242_N326_K0.5751.00
185_R386_F0.5741.00
68_D94_L0.5741.00
240_S324_R0.5731.00
53_G132_F0.5721.00
162_D199_D0.5721.00
219_L253_D0.5711.00
152_F156_G0.5711.00
75_V82_A0.5701.00
286_D319_R0.5701.00
253_D256_S0.5671.00
393_G396_S0.5671.00
339_G343_A0.5661.00
15_E74_I0.5651.00
87_F112_K0.5621.00
232_L395_P0.5611.00
229_A265_N0.5601.00
282_I285_F0.5601.00
351_C355_L0.5591.00
151_L154_A0.5581.00
347_A351_C0.5581.00
162_D165_A0.5571.00
302_V323_D0.5571.00
46_H140_T0.5571.00
230_H267_L0.5561.00
291_A295_L0.5551.00
97_L101_E0.5541.00
90_A94_L0.5531.00
187_I387_A0.5531.00
191_E305_D0.5511.00
260_N263_K0.5511.00
295_L300_I0.5511.00
222_K257_F0.5501.00
258_L300_I0.5491.00
159_K196_K0.5481.00
222_K226_F0.5471.00
218_D221_S0.5471.00
189_A237_K0.5461.00
31_N38_G0.5451.00
173_H224_L0.5441.00
64_A97_L0.5441.00
12_R58_V0.5431.00
215_Y219_L0.5431.00
157_S161_L0.5411.00
95_F108_A0.5401.00
316_L320_F0.5391.00
51_L57_Q0.5381.00
386_F389_A0.5371.00
254_A257_F0.5361.00
274_V302_V0.5361.00
26_I30_R0.5351.00
153_I157_S0.5341.00
222_K243_L0.5331.00
115_S125_A0.5331.00
24_V38_G0.5331.00
209_R279_S0.5321.00
368_E371_M0.5291.00
212_G246_M0.5291.00
59_K62_Y0.5281.00
119_L124_R0.5281.00
209_R244_L0.5261.00
332_I345_I0.5251.00
286_D290_E0.5251.00
270_E301_S0.5251.00
247_T254_A0.5241.00
269_P300_I0.5241.00
163_S196_K0.5231.00
24_V28_N0.5231.00
285_F319_R0.5231.00
335_V342_Q0.5221.00
96_D100_H0.5211.00
152_F155_D0.5211.00
155_D159_K0.5201.00
318_S340_P0.5191.00
243_L247_T0.5181.00
170_I227_T0.5171.00
344_I348_I0.5171.00
324_R357_I0.5161.00
11_I58_V0.5151.00
30_R79_C0.5141.00
164_C168_P0.5121.00
336_H339_G0.5101.00
293_K296_K0.5101.00
129_F150_W0.5091.00
262_I267_L0.5071.00
17_V128_F0.5071.00
314_L348_I0.5071.00
92_M142_F0.5061.00
282_I288_F0.5051.00
226_F262_I0.5011.00
335_V373_L0.5011.00
50_L156_G0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4rnhA 2 0.9628 100 0.118 Contact Map
4j40A 2 0.6055 100 0.14 Contact Map
3pjxA 1 0.9429 100 0.159 Contact Map
3gfzA 2 0.9181 100 0.23 Contact Map
4hjfA 1 0.6203 100 0.269 Contact Map
4hu4A 1 0.5856 100 0.347 Contact Map
2r6oA 2 0.6129 100 0.348 Contact Map
4rniA 2 0.6104 100 0.353 Contact Map
4kieA 1 0.6278 100 0.357 Contact Map
3sy8A 6 0.9057 100 0.382 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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