GREMLIN Database
FLGF - Flagellar basal-body rod protein FlgF
UniProt: P75938 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14270
Length: 251 (242)
Sequences: 5723 (4191)
Seq/√Len: 269.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
88_L105_I3.6191.00
80_V137_I3.2051.00
138_A142_T3.1031.00
108_D112_Q3.0321.00
99_Y166_A3.0051.00
174_G180_R2.9561.00
114_T118_H2.9521.00
71_M205_V2.8891.00
112_Q119_P2.8461.00
145_A158_V2.8221.00
71_M81_A2.6991.00
89_A164_V2.6971.00
85_D165_K2.5461.00
172_Q180_R2.3481.00
66_M87_W2.2471.00
92_T98_G2.2341.00
142_T157_P2.2321.00
202_M205_V2.2291.00
239_D242_G2.1821.00
90_V100_T2.1401.00
37_Q173_R2.0861.00
172_Q182_S2.0751.00
72_D208_G2.0691.00
181_L187_A2.0511.00
36_A216_A2.0311.00
89_A97_E1.9581.00
92_T96_S1.9411.00
98_G174_G1.9271.00
73_Y205_V1.9081.00
19_Q220_M1.8991.00
18_Q223_S1.8911.00
80_V201_V1.8881.00
91_Q121_I1.8581.00
102_N177_G1.8551.00
98_G178_I1.8501.00
4_A237_V1.8011.00
41_L45_P1.7721.00
26_L213_A1.7651.00
83_Q202_M1.7421.00
105_I120_V1.7421.00
164_V190_G1.7221.00
82_L163_L1.7181.00
22_T223_S1.6941.00
82_L199_L1.6721.00
18_Q219_D1.6581.00
232_K235_S1.6461.00
19_Q223_S1.6231.00
144_S157_P1.6071.00
113_L120_V1.5971.00
212_N215_A1.5851.00
137_I143_I1.5831.00
138_A144_S1.5811.00
215_A219_D1.5791.00
74_T79_D1.5761.00
90_V114_T1.5411.00
14_Q57_V1.5191.00
64_A173_R1.5161.00
32_P206_L1.5051.00
78_L135_I1.4991.00
92_T118_H1.4881.00
15_T226_R1.4671.00
80_V161_L1.4481.00
147_N150_D1.4201.00
80_V88_L1.4181.00
219_D222_A1.4081.00
112_Q128_A1.3851.00
139_A200_R1.3781.00
32_P102_N1.3761.00
160_R192_V1.3721.00
142_T160_R1.3721.00
166_A181_L1.3601.00
22_T220_M1.3571.00
98_G180_R1.3571.00
216_A219_D1.3431.00
8_A233_V1.3371.00
122_G162_K1.3311.00
93_A118_H1.3301.00
127_I130_P1.3221.00
102_N115_I1.3171.00
70_K208_G1.3131.00
21_V39_N1.3101.00
16_L20_A1.3001.00
36_A215_A1.2951.00
88_L161_L1.2871.00
41_L44_V1.2841.00
146_L155_V1.2541.00
90_V178_I1.2491.00
165_K198_T1.2351.00
39_N59_A1.2241.00
89_A121_I1.2221.00
235_S238_D1.2201.00
118_H178_I1.1991.00
78_L113_L1.1931.00
36_A65_D1.1861.00
37_Q64_A1.1861.00
29_A213_A1.1851.00
76_R79_D1.1841.00
44_V48_G1.1761.00
132_G145_A1.1681.00
123_E160_R1.1571.00
90_V98_G1.1571.00
90_V105_I1.1461.00
38_L63_G1.1451.00
99_Y188_T1.1351.00
11_A230_Q1.1291.00
17_N59_A1.1231.00
32_P104_S1.1131.00
80_V203_S1.1091.00
122_G159_G1.1071.00
182_S185_T1.1051.00
34_F37_Q1.1011.00
24_S34_F1.0881.00
133_A148_P1.0571.00
174_G178_I1.0491.00
130_P133_A1.0481.00
144_S155_V1.0421.00
185_T188_T1.0321.00
89_A190_G1.0221.00
242_G246_Q1.0211.00
116_Q176_D1.0171.00
81_A205_V1.0151.00
238_D241_A1.0131.00
194_Q200_R1.0131.00
96_S180_R1.0121.00
15_T223_S1.0061.00
97_E121_I1.0011.00
243_R247_L0.9901.00
89_A162_K0.9901.00
91_Q119_P0.9871.00
66_M179_F0.9861.00
22_T216_A0.9791.00
114_T120_V0.9701.00
120_V161_L0.9691.00
170_E182_S0.9651.00
21_V34_F0.9641.00
78_L107_V0.9631.00
134_E146_L0.9631.00
83_Q200_R0.9541.00
43_A46_V0.9451.00
25_N216_A0.9451.00
38_L65_D0.9401.00
163_L198_T0.9221.00
9_M12_A0.9201.00
42_R58_T0.9201.00
18_Q22_T0.9151.00
52_P173_R0.9151.00
166_A171_V0.9141.00
127_I151_P0.9121.00
121_I126_P0.9111.00
14_Q41_L0.9081.00
15_T18_Q0.9041.00
82_L86_G0.9021.00
225_R228_E0.8971.00
77_P134_E0.8961.00
181_L185_T0.8881.00
114_T178_I0.8811.00
92_T95_G0.8781.00
113_L127_I0.8661.00
76_R106_Q0.8651.00
163_L199_L0.8611.00
19_Q240_N0.8611.00
130_P145_A0.8501.00
80_V105_I0.8491.00
126_P130_P0.8481.00
36_A210_N0.8451.00
129_V132_G0.8340.99
173_R179_F0.8280.99
241_A245_N0.8250.99
147_N151_P0.8250.99
91_Q97_E0.8230.99
147_N156_A0.8220.99
139_A202_M0.8210.99
97_E162_K0.8200.99
192_V195_A0.8140.99
23_A233_V0.8100.99
42_R60_S0.8060.99
130_P148_P0.8010.99
106_Q115_I0.8000.99
8_A234_I0.7970.99
40_A63_G0.7820.99
133_A145_A0.7820.99
11_A15_T0.7810.99
243_R246_Q0.7780.99
19_Q224_A0.7750.99
79_D104_S0.7630.99
126_P129_V0.7590.99
2_D241_A0.7570.99
93_A96_S0.7530.99
161_L201_V0.7510.99
3_H55_T0.7460.99
214_V217_M0.7440.99
140_D160_R0.7430.99
86_G199_L0.7420.99
194_Q197_P0.7390.99
104_S115_I0.7320.99
46_V54_R0.7310.99
108_D119_P0.7290.99
166_A188_T0.7280.99
121_I162_K0.7260.99
217_M235_S0.7240.99
124_A127_I0.7240.99
196_D199_L0.7230.99
91_Q95_G0.7200.98
16_L227_F0.7170.98
68_P84_Q0.7170.98
129_V133_A0.7130.98
12_A231_M0.7120.98
87_W179_F0.7110.98
80_V120_V0.7020.98
40_A61_T0.7010.98
100_T114_T0.7010.98
143_I159_G0.7000.98
132_G147_N0.6980.98
237_V240_N0.6940.98
122_G126_P0.6930.98
235_S239_D0.6920.98
125_G162_K0.6920.98
146_L150_D0.6900.98
34_F38_L0.6890.98
5_I241_A0.6860.98
10_G14_Q0.6840.98
13_S212_N0.6840.98
18_Q222_A0.6830.98
8_A230_Q0.6780.98
82_L88_L0.6780.98
90_V118_H0.6750.98
32_P208_G0.6720.98
166_A186_Q0.6690.98
86_G101_R0.6680.98
82_L201_V0.6670.98
6_Y222_A0.6650.97
95_G98_G0.6640.97
38_L41_L0.6640.97
5_I234_I0.6610.97
71_M202_M0.6600.97
228_E232_K0.6530.97
56_L59_A0.6470.97
234_I238_D0.6460.97
7_T56_L0.6410.97
2_D240_N0.6360.97
68_P83_Q0.6300.97
107_V120_V0.6290.96
213_A228_E0.6270.96
236_S239_D0.6250.96
165_K189_R0.6230.96
88_L103_G0.6220.96
194_Q198_T0.6200.96
77_P203_S0.6180.96
225_R238_D0.6180.96
53_T62_P0.6140.96
4_A233_V0.6130.96
170_E185_T0.6120.96
193_L201_V0.6110.96
130_P149_G0.6100.96
13_S215_A0.6100.96
86_G163_L0.6090.96
211_V216_A0.6090.96
238_D242_G0.6080.96
41_L48_G0.6050.96
51_L62_P0.6020.95
234_I247_L0.6010.95
240_N244_A0.6000.95
68_P71_M0.6000.95
40_A44_V0.6000.95
119_P126_P0.6000.95
231_M240_N0.5980.95
106_Q109_P0.5980.95
227_F240_N0.5970.95
104_S206_L0.5950.95
3_H20_A0.5950.95
113_L161_L0.5940.95
4_A7_T0.5920.95
163_L201_V0.5920.95
3_H47_E0.5900.95
91_Q126_P0.5900.95
214_V222_A0.5890.95
103_G206_L0.5880.95
235_S242_G0.5860.95
244_A247_L0.5850.95
112_Q126_P0.5840.95
126_P147_N0.5800.95
227_F231_M0.5790.94
33_G179_F0.5790.94
168_G171_V0.5790.94
131_E157_P0.5780.94
17_N21_V0.5780.94
39_N62_P0.5750.94
12_A15_T0.5740.94
21_V62_P0.5730.94
25_N34_F0.5720.94
72_D206_L0.5720.94
89_A161_L0.5710.94
213_A229_M0.5700.94
13_S17_N0.5690.94
233_V237_V0.5690.94
170_E184_E0.5690.94
182_S186_Q0.5680.94
162_K190_G0.5680.94
163_L193_L0.5670.94
108_D111_G0.5670.94
100_T103_G0.5660.94
15_T218_S0.5640.94
89_A99_Y0.5620.94
43_A54_R0.5590.93
17_N20_A0.5590.93
135_I145_A0.5580.93
159_G162_K0.5580.93
194_Q199_L0.5560.93
90_V120_V0.5540.93
129_V135_I0.5520.93
88_L137_I0.5520.93
9_M15_T0.5500.93
28_N31_T0.5500.93
42_R45_P0.5490.93
82_L87_W0.5420.92
139_A143_I0.5410.92
32_P72_D0.5410.92
10_G57_V0.5400.92
15_T227_F0.5380.92
165_K196_D0.5380.92
15_T19_Q0.5360.92
26_L216_A0.5350.92
143_I158_V0.5340.92
80_V204_G0.5340.92
35_R207_E0.5340.92
181_L188_T0.5340.92
45_P48_G0.5310.92
47_E55_T0.5300.91
59_A62_P0.5290.91
14_Q59_A0.5270.91
21_V59_A0.5260.91
76_R206_L0.5240.91
181_L184_E0.5230.91
6_Y10_G0.5230.91
107_V111_G0.5220.91
2_D7_T0.5190.91
7_T20_A0.5190.91
138_A157_P0.5180.90
221_I224_A0.5170.90
145_A159_G0.5160.90
228_E235_S0.5160.90
137_I203_S0.5150.90
107_V113_L0.5150.90
122_G125_G0.5140.90
98_G118_H0.5120.90
123_E190_G0.5090.90
7_T12_A0.5090.90
129_V158_V0.5090.90
216_A224_A0.5080.90
12_A234_I0.5080.90
229_M232_K0.5070.90
155_V183_A0.5070.90
35_R69_G0.5060.89
190_G198_T0.5060.89
105_I115_I0.5040.89
88_L102_N0.5040.89
236_S240_N0.5030.89
5_I11_A0.5020.89
217_M243_R0.5010.89
69_G210_N0.5010.89
3_H12_A0.5000.89
92_T119_P0.5000.89
224_A231_M0.5000.89
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3a69A 3 0.7331 100 0.116 Contact Map
1wlgA 2 0.5179 100 0.591 Contact Map
4ut1A 1 0.5339 99.8 0.715 Contact Map
1oryB 2 0.1594 16.2 0.958 Contact Map
2d4yA 1 0.741 11.4 0.961 Contact Map
2x5pA 1 0.3944 11.2 0.961 Contact Map
4zcnA 3 0.3347 10.7 0.961 Contact Map
4tkcA 2 0.4223 5 0.967 Contact Map
4dwlA 3 0.1275 2.2 0.972 Contact Map
1nh2B 1 0.0876 1.9 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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