GREMLIN Database
EFEU - Putative inactive ferrous iron permease EfeU
UniProt: P75901 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13860
Length: 276 (262)
Sequences: 1469 (931)
Seq/√Len: 57.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
5_F160_T6.3881.00
98_K102_E4.5141.00
70_L74_I4.5071.00
16_A165_G3.8561.00
200_I250_V3.6361.00
44_L120_V3.4711.00
26_L38_M3.3471.00
53_G132_L2.9551.00
233_M237_F2.8651.00
199_A203_F2.7081.00
23_A35_D2.6361.00
47_A51_G2.6241.00
166_F170_W2.5701.00
216_A245_V2.4731.00
76_A199_A2.4041.00
84_T254_Y2.3561.00
32_G35_D2.3541.00
245_V249_A2.2201.00
249_A253_I2.1561.00
230_G239_Y2.1461.00
22_I123_V2.0621.00
80_V196_A2.0451.00
55_F59_T1.9941.00
7_I134_S1.9931.00
159_A163_V1.9881.00
49_C131_G1.9811.00
70_L208_L1.9671.00
70_L206_A1.9391.00
19_V42_V1.9281.00
227_S230_G1.8991.00
215_I242_A1.8831.00
125_F235_G1.8390.99
36_C40_I1.8380.99
243_P248_V1.8100.99
52_L128_A1.7830.99
13_L164_L1.7690.99
23_A38_M1.7680.99
155_M159_A1.7590.99
179_A182_K1.7310.99
163_V167_L1.6800.99
40_I44_L1.6260.99
60_T71_F1.6130.99
11_E14_E1.6100.99
160_T163_V1.5880.98
215_I243_P1.5850.98
51_G55_F1.5740.98
83_L188_I1.5480.98
77_V199_A1.5350.98
77_V203_F1.5340.98
105_V119_L1.5000.98
196_A250_V1.4980.98
5_F156_L1.4760.98
108_A118_A1.4630.97
39_W42_V1.4560.97
51_G54_I1.4490.97
219_M222_V1.4450.97
32_G36_C1.4410.97
219_M239_Y1.4150.97
169_Y191_V1.4040.97
8_M157_G1.4030.97
9_L164_L1.3950.97
259_L263_A1.3920.97
45_A140_A1.3900.96
102_E106_D1.3870.96
197_A247_E1.3790.96
92_K95_R1.3570.96
49_C53_G1.3410.96
165_G169_Y1.3350.96
27_K35_D1.3220.95
4_P150_P1.2910.95
84_T192_A1.2900.95
142_F150_P1.2840.94
201_R247_E1.2760.94
21_L97_V1.2710.94
138_L154_A1.2650.94
76_A198_G1.2630.94
242_A248_V1.2600.94
87_V188_I1.2480.94
9_L160_T1.2440.94
56_I135_V1.2380.93
257_P260_V1.2320.93
26_L39_W1.2290.93
234_E247_E1.2230.93
228_L244_S1.2180.93
69_E205_E1.1930.92
230_G234_E1.1850.92
182_K186_L1.1690.91
73_G203_F1.1680.91
59_T166_F1.1660.91
44_L124_F1.1640.91
87_V91_R1.1520.91
100_Q104_A1.1460.90
225_T239_Y1.1290.90
215_I218_D1.1250.90
87_V161_A1.1240.89
150_P154_A1.1240.89
20_S169_Y1.1180.89
202_A205_E1.1180.89
110_Q187_F1.1160.89
23_A27_K1.1100.89
240_Q243_P1.1040.89
55_F216_A1.1040.89
82_I122_M1.1010.88
230_G243_P1.1000.88
80_V199_A1.0940.88
170_W174_R1.0880.88
14_E130_E1.0860.88
201_R240_Q1.0750.87
178_G182_K1.0730.87
25_Y106_D1.0670.87
260_V264_L1.0660.87
130_E133_E1.0630.87
201_R205_E1.0550.86
51_G121_M1.0530.86
60_T128_A1.0530.86
51_G112_G1.0410.85
25_Y118_A1.0380.85
8_M138_L1.0280.85
45_A49_C1.0260.85
39_W180_F1.0260.85
83_L136_F1.0230.84
16_A20_S1.0220.84
10_R14_E1.0190.84
90_M175_L1.0180.84
37_V117_W1.0170.84
152_L156_L1.0100.84
28_R102_E1.0070.84
38_M210_N1.0060.83
48_L124_F1.0060.83
252_F256_I1.0050.83
203_F208_L0.9970.83
234_E240_Q0.9960.83
28_R180_F0.9880.82
8_M58_E0.9810.82
248_V252_F0.9730.81
84_T193_A0.9680.81
27_K32_G0.9600.80
34_X109_L0.9590.80
54_I58_E0.9540.80
69_E80_V0.9530.80
99_V103_Q0.9490.80
123_V126_A0.9480.80
15_A126_A0.9430.79
43_L47_A0.9420.79
240_Q261_A0.9390.79
219_M243_P0.9390.79
52_L132_L0.9350.79
33_M47_A0.9350.79
12_G153_G0.9250.78
215_I248_V0.9210.78
46_A162_V0.9150.77
68_Q72_E0.9140.77
197_A244_S0.9120.77
214_E245_V0.9100.77
94_S185_S0.9070.77
40_I120_V0.9040.76
57_N60_T0.8970.76
248_V261_A0.8960.76
17_L140_A0.8900.75
25_Y180_F0.8900.75
187_F258_A0.8890.75
13_L177_L0.8780.74
204_H244_S0.8740.74
12_G161_A0.8740.74
16_A168_L0.8730.74
77_V81_V0.8710.74
72_E205_E0.8670.73
256_I260_V0.8600.73
51_G162_V0.8600.73
59_T63_F0.8590.73
45_A124_F0.8580.72
77_V144_Q0.8540.72
225_T241_E0.8450.71
84_T88_F0.8450.71
3_V76_A0.8360.71
111_R148_I0.8340.70
235_G251_W0.8280.70
25_Y195_L0.8210.69
195_L198_G0.8200.69
8_M134_S0.8180.69
187_F237_F0.8180.69
119_L207_G0.8160.69
3_V165_G0.8140.68
9_L12_G0.8130.68
225_T230_G0.8120.68
218_D221_A0.8070.68
204_H210_N0.8070.68
156_L160_T0.8060.68
14_E129_R0.8050.68
90_M189_L0.8040.68
17_L81_V0.8000.67
135_V157_G0.8000.67
107_S111_R0.7990.67
42_V127_V0.7990.67
90_M109_L0.7970.67
219_M242_A0.7960.67
89_W181_F0.7960.67
236_I239_Y0.7950.67
222_V239_Y0.7930.66
148_I152_L0.7930.66
224_S227_S0.7920.66
68_Q80_V0.7890.66
223_L239_Y0.7860.66
218_D230_G0.7840.66
73_G199_A0.7830.65
210_N213_Q0.7810.65
261_A264_L0.7810.65
101_L126_A0.7790.65
161_A261_A0.7790.65
18_I65_Q0.7770.65
153_G247_E0.7710.64
47_A159_A0.7690.64
2_F136_F0.7680.64
186_L259_L0.7650.64
42_V131_G0.7650.64
2_F168_L0.7610.63
72_E139_L0.7600.63
68_Q184_T0.7590.63
250_V253_I0.7550.63
14_E133_E0.7530.62
200_I204_H0.7510.62
27_K31_R0.7490.62
155_M187_F0.7450.62
160_T167_L0.7450.62
85_W181_F0.7410.61
8_M20_S0.7390.61
39_W131_G0.7300.60
20_S168_L0.7290.60
77_V132_L0.7270.60
139_L143_Q0.7240.59
32_G51_G0.7230.59
94_S127_V0.7230.59
20_S157_G0.7220.59
5_F154_A0.7210.59
26_L36_C0.7180.59
83_L141_A0.7170.59
30_Q77_V0.7170.59
41_G122_M0.7160.59
254_Y258_A0.7140.58
56_I63_F0.7140.58
156_L183_W0.7130.58
247_E255_L0.7130.58
27_K162_V0.7080.58
156_L258_A0.7080.58
21_L161_A0.7040.57
200_I246_S0.7020.57
148_I155_M0.7010.57
96_N99_V0.7000.57
29_T36_C0.6970.56
148_I151_P0.6960.56
22_I120_V0.6940.56
79_A82_I0.6910.56
45_A192_A0.6900.56
219_M223_L0.6870.55
122_M192_A0.6870.55
230_G233_M0.6820.55
72_E202_A0.6810.55
38_M119_L0.6810.55
188_I191_V0.6800.55
87_V185_S0.6780.54
168_L180_F0.6750.54
97_V100_Q0.6730.54
249_A260_V0.6720.54
99_V102_E0.6720.54
200_I244_S0.6720.54
32_G103_Q0.6670.53
4_P8_M0.6670.53
94_S98_K0.6660.53
253_I257_P0.6640.53
62_E136_F0.6630.53
2_F5_F0.6600.52
66_K132_L0.6570.52
69_E77_V0.6530.52
140_A205_E0.6530.52
116_G120_V0.6480.51
110_Q113_N0.6470.51
42_V56_I0.6460.51
79_A137_F0.6450.51
197_A239_Y0.6430.51
129_R133_E0.6420.50
12_G16_A0.6390.50
120_V123_V0.6330.49
44_L81_V0.6320.49
244_S249_A0.6280.49
46_A158_L0.6280.49
42_V157_G0.6250.49
25_Y189_L0.6240.49
224_S229_F0.6240.49
140_A158_L0.6220.48
204_H246_S0.6210.48
74_I78_I0.6190.48
180_F195_L0.6180.48
104_A199_A0.6170.48
54_I103_Q0.6150.48
198_G202_A0.6130.47
37_V116_G0.6100.47
15_A19_V0.6080.47
232_L260_V0.6080.47
76_A202_A0.6070.47
43_L208_L0.6050.46
68_Q203_F0.6040.46
33_M81_V0.6030.46
137_F197_A0.6030.46
156_L164_L0.6020.46
20_S34_X0.6010.46
234_E239_Y0.6000.46
229_F232_L0.5980.46
90_M97_V0.5980.46
96_N100_Q0.5980.46
81_V85_W0.5960.45
79_A90_M0.5950.45
132_L136_F0.5950.45
26_L31_R0.5950.45
228_L231_T0.5940.45
51_G216_A0.5930.45
124_F128_A0.5930.45
223_L230_G0.5930.45
18_I29_T0.5920.45
224_S240_Q0.5910.45
110_Q164_L0.5900.45
78_I188_I0.5860.44
112_G116_G0.5860.44
155_M218_D0.5850.44
26_L30_Q0.5830.44
68_Q87_V0.5810.44
227_S231_T0.5760.43
41_G123_V0.5760.43
229_F238_G0.5760.43
119_L195_L0.5760.43
228_L238_G0.5750.43
82_I175_L0.5750.43
122_M126_A0.5710.43
45_A205_E0.5690.43
258_A262_F0.5680.42
36_C121_M0.5680.42
110_Q249_A0.5670.42
135_V188_I0.5660.42
89_W153_G0.5600.42
47_A113_N0.5590.41
46_A107_S0.5580.41
51_G110_Q0.5570.41
74_I190_F0.5570.41
193_A251_W0.5530.41
12_G206_A0.5530.41
33_M109_L0.5500.40
83_L199_A0.5500.40
237_F260_V0.5490.40
64_P67_E0.5490.40
65_Q69_E0.5490.40
53_G56_I0.5480.40
23_A165_G0.5460.40
180_F184_T0.5440.40
77_V226_H0.5430.40
106_D119_L0.5430.40
257_P263_A0.5420.40
82_I187_F0.5420.40
3_V145_D0.5400.39
153_G229_F0.5380.39
83_L87_V0.5380.39
258_A261_A0.5370.39
218_D222_V0.5360.39
44_L99_V0.5350.39
246_S250_V0.5340.39
167_L254_Y0.5330.39
119_L174_R0.5330.39
102_E190_F0.5310.38
52_L63_F0.5250.38
122_M136_F0.5230.38
7_I112_G0.5230.38
222_V241_E0.5210.37
31_R174_R0.5170.37
134_S201_R0.5150.37
128_A194_G0.5150.37
158_L196_A0.5150.37
46_A103_Q0.5140.37
50_L159_A0.5140.37
19_V77_V0.5140.37
104_A118_A0.5140.37
89_W134_S0.5120.37
101_L105_V0.5110.36
52_L81_V0.5100.36
52_L82_I0.5070.36
44_L50_L0.5070.36
230_G240_Q0.5060.36
197_A243_P0.5060.36
221_A225_T0.5050.36
18_I192_A0.5030.36
235_G247_E0.5010.35
30_Q179_A0.5010.35
225_T234_E0.5010.35
249_A261_A0.5010.35
226_H258_A0.5010.35
7_I10_R0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2wswA 3 0.6232 68.2 0.946 Contact Map
2rddB 1 0.1232 26.6 0.958 Contact Map
3o7qA 1 0.9348 19 0.961 Contact Map
2ks1B 1 0.1594 18.6 0.961 Contact Map
3m73A 3 0.6993 18.1 0.961 Contact Map
4m64A 1 0.9638 17.1 0.962 Contact Map
4czbA 2 0.8732 14.6 0.963 Contact Map
4j05A 3 0.9167 14.4 0.963 Contact Map
2jwaA 2 0.1522 13.5 0.963 Contact Map
3wdoA 1 0.9348 12.5 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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