ELFG - ECOLI - GREMLIN Contacts

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ELFG - Uncharacterized fimbrial-like protein ElfG

UniProt:	P75858	Sequences: MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYIGVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVYLGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASGNSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSLGSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSLTATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVSTTGVPPETGRFEATATVRINFD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13712
Length:	356 (324)
Sequences:	224 (184)
Seq/√Len:	10.2

ECOLI - ELFG YRAK SFMH

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
300_T	331_Y	4.849	1.00
236_V	345_F	4.005	0.99
336_T	340_P	3.934	0.99
189_E	206_V	3.880	0.99
82_C	146_C	3.619	0.98
275_S	354_N	3.469	0.97
80_C	175_G	3.000	0.93
301_D	305_N	2.874	0.92
172_P	213_G	2.804	0.90
120_L	187_L	2.765	0.90
278_A	288_I	2.695	0.89
130_V	207_L	2.542	0.85
173_F	215_I	2.509	0.85
292_R	340_P	2.473	0.84
167_L	215_I	2.470	0.84
231_V	256_L	2.460	0.83
180_P	213_G	2.420	0.82
115_A	170_L	2.359	0.81
287_A	297_V	2.307	0.79
114_L	169_I	2.296	0.79
100_L	124_G	2.238	0.77
214_T	220_S	2.173	0.75
104_G	114_L	2.150	0.74
18_A	63_V	2.148	0.74
219_G	292_R	2.060	0.70
106_N	117_K	2.047	0.70
334_S	340_P	2.043	0.70
122_V	137_T	2.008	0.68
76_Y	160_G	2.002	0.68
173_F	219_G	1.989	0.68
290_T	347_A	1.957	0.66
272_V	354_N	1.902	0.64
80_C	180_P	1.877	0.63
93_A	96_A	1.835	0.61
219_G	334_S	1.822	0.61
307_I	312_G	1.805	0.60
292_R	336_T	1.790	0.59
173_F	190_C	1.775	0.58
18_A	21_L	1.756	0.58
119_L	164_S	1.738	0.57
206_V	228_T	1.737	0.57
270_V	326_A	1.734	0.57
226_G	229_I	1.730	0.56
295_V	342_T	1.725	0.56
319_P	354_N	1.708	0.55
107_W	206_V	1.708	0.55
46_V	184_L	1.707	0.55
48_F	184_L	1.703	0.55
212_S	274_L	1.701	0.55
174_V	222_S	1.692	0.55
100_L	108_Y	1.678	0.54
122_V	132_F	1.647	0.53
172_P	266_N	1.637	0.52
109_K	170_L	1.636	0.52
233_L	345_F	1.620	0.51
297_V	335_T	1.593	0.50
179_I	294_G	1.592	0.50
188_Y	206_V	1.558	0.48
137_T	160_G	1.556	0.48
70_W	163_G	1.546	0.48
160_G	163_G	1.510	0.46
58_N	82_C	1.498	0.46
130_V	156_Q	1.479	0.45
122_V	163_G	1.471	0.44
175_G	295_V	1.468	0.44
36_H	39_Q	1.460	0.44
137_T	159_S	1.450	0.43
231_V	347_A	1.448	0.43
167_L	264_T	1.447	0.43
221_C	262_C	1.426	0.42
117_K	292_R	1.421	0.42
311_G	331_Y	1.419	0.42
229_I	324_S	1.416	0.42
163_G	209_Y	1.411	0.42
111_N	205_P	1.409	0.42
179_I	213_G	1.409	0.42
190_C	340_P	1.401	0.41
287_A	295_V	1.399	0.41
218_L	345_F	1.398	0.41
219_G	337_G	1.394	0.41
160_G	207_L	1.363	0.40
251_A	324_S	1.359	0.39
218_L	236_V	1.344	0.39
299_V	305_N	1.337	0.38
103_L	294_G	1.334	0.38
289_K	297_V	1.323	0.38
240_N	250_G	1.318	0.38
224_N	229_I	1.315	0.37
22_M	25_C	1.310	0.37
20_V	26_A	1.304	0.37
222_S	227_E	1.303	0.37
48_F	182_M	1.302	0.37
80_C	295_V	1.302	0.37
226_G	294_G	1.301	0.37
281_D	348_T	1.286	0.36
221_C	332_P	1.275	0.36
296_G	333_V	1.274	0.36
40_G	233_L	1.272	0.36
352_R	355_F	1.269	0.35
324_S	350_T	1.268	0.35
285_P	305_N	1.266	0.35
80_C	108_Y	1.258	0.35
106_N	115_A	1.256	0.35
227_E	347_A	1.247	0.35
184_L	187_L	1.243	0.34
265_G	322_A	1.233	0.34
35_C	287_A	1.222	0.33
226_G	296_G	1.205	0.33
113_Y	260_V	1.194	0.32
309_P	342_T	1.191	0.32
241_F	334_S	1.190	0.32
217_S	294_G	1.188	0.32
20_V	56_E	1.184	0.32
22_M	26_A	1.178	0.32
203_G	235_D	1.165	0.31
33_G	163_G	1.164	0.31
20_V	25_C	1.159	0.31
231_V	339_P	1.155	0.31
126_S	138_G	1.154	0.31
47_N	112_D	1.149	0.31
182_M	213_G	1.139	0.30
186_R	208_V	1.135	0.30
185_A	207_L	1.133	0.30
118_V	211_L	1.123	0.30
125_S	128_T	1.123	0.30
219_G	265_G	1.118	0.29
19_A	303_Q	1.108	0.29
116_A	185_A	1.106	0.29
35_C	80_C	1.106	0.29
19_A	25_C	1.101	0.29
82_C	203_G	1.099	0.29
118_V	185_A	1.097	0.29
92_W	118_V	1.087	0.28
233_L	256_L	1.084	0.28
218_L	227_E	1.083	0.28
262_C	332_P	1.079	0.28
314_L	322_A	1.077	0.28
126_S	184_L	1.068	0.27
258_I	295_V	1.067	0.27
175_G	342_T	1.055	0.27
274_L	316_L	1.049	0.27
324_S	334_S	1.048	0.27
108_Y	160_G	1.048	0.27
176_S	355_F	1.045	0.27
106_N	354_N	1.045	0.27
118_V	191_Y	1.040	0.26
107_W	168_K	1.040	0.26
110_I	229_I	1.039	0.26
297_V	340_P	1.039	0.26
286_Q	333_V	1.038	0.26
215_I	301_D	1.037	0.26
172_P	314_L	1.035	0.26
274_L	353_I	1.033	0.26
219_G	340_P	1.031	0.26
237_F	240_N	1.025	0.26
32_S	307_I	1.023	0.26
122_V	160_G	1.022	0.26
102_Y	294_G	1.021	0.26
271_N	342_T	1.015	0.26
73_S	100_L	1.015	0.26
89_S	276_L	1.012	0.25
335_T	340_P	1.009	0.25
81_D	295_V	1.001	0.25
257_A	328_M	1.001	0.25
84_K	114_L	1.000	0.25
327_R	349_A	1.000	0.25
48_F	65_D	0.999	0.25
137_T	189_E	0.999	0.25
324_S	331_Y	0.998	0.25
209_Y	268_G	0.997	0.25
348_T	351_V	0.991	0.25
161_N	294_G	0.990	0.25
174_V	297_V	0.989	0.25
25_C	29_A	0.985	0.24
37_T	206_V	0.985	0.24
297_V	350_T	0.983	0.24
89_S	278_A	0.981	0.24
278_A	286_Q	0.980	0.24
243_V	334_S	0.976	0.24
275_S	340_P	0.975	0.24
120_L	167_L	0.972	0.24
307_I	334_S	0.966	0.24
247_K	253_T	0.957	0.24
61_G	349_A	0.955	0.23
276_L	330_V	0.954	0.23
94_F	187_L	0.952	0.23
114_L	173_F	0.951	0.23
111_N	173_F	0.950	0.23
132_F	159_S	0.946	0.23
130_V	134_N	0.942	0.23
115_A	215_I	0.941	0.23
117_K	177_V	0.934	0.23
251_A	297_V	0.934	0.23
251_A	334_S	0.930	0.23
134_N	137_T	0.927	0.23
178_V	278_A	0.926	0.23
231_V	234_G	0.924	0.22
290_T	348_T	0.922	0.22
173_F	213_G	0.921	0.22
273_N	327_R	0.920	0.22
175_G	340_P	0.920	0.22
298_V	351_V	0.917	0.22
341_E	346_E	0.916	0.22
270_V	275_S	0.914	0.22
258_I	292_R	0.909	0.22
61_G	74_N	0.909	0.22
28_T	295_V	0.908	0.22
167_L	331_Y	0.907	0.22
334_S	337_G	0.905	0.22
101_V	184_L	0.905	0.22
72_E	189_E	0.902	0.22
293_P	353_I	0.896	0.22
44_I	191_Y	0.895	0.22
289_K	335_T	0.891	0.21
245_G	336_T	0.891	0.21
166_S	291_S	0.890	0.21
99_P	198_S	0.889	0.21
173_F	180_P	0.881	0.21
25_C	28_T	0.878	0.21
49_G	94_F	0.874	0.21
114_L	213_G	0.874	0.21
256_L	328_M	0.869	0.21
96_A	279_T	0.869	0.21
54_E	171_Q	0.867	0.21
314_L	326_A	0.864	0.21
219_G	241_F	0.862	0.21
107_W	178_V	0.861	0.20
305_N	333_V	0.860	0.20
307_I	310_A	0.858	0.20
265_G	268_G	0.854	0.20
184_L	272_V	0.854	0.20
70_W	185_A	0.854	0.20
316_L	352_R	0.850	0.20
107_W	117_K	0.847	0.20
221_C	343_G	0.847	0.20
295_V	352_R	0.847	0.20
215_I	265_G	0.844	0.20
100_L	207_L	0.838	0.20
132_F	163_G	0.830	0.20
236_V	291_S	0.827	0.19
33_G	146_C	0.827	0.19
292_R	337_G	0.825	0.19
161_N	233_L	0.825	0.19
46_V	211_L	0.824	0.19
166_S	185_A	0.821	0.19
109_K	330_V	0.819	0.19
110_I	115_A	0.815	0.19
169_I	334_S	0.814	0.19
170_L	304_N	0.814	0.19
63_V	132_F	0.813	0.19
109_K	112_D	0.811	0.19
19_A	178_V	0.810	0.19
108_Y	168_K	0.807	0.19
316_L	350_T	0.807	0.19
190_C	292_R	0.805	0.19
250_G	284_Y	0.804	0.19
223_V	236_V	0.801	0.19
113_Y	352_R	0.801	0.19
283_S	336_T	0.799	0.19
267_A	279_T	0.797	0.19
217_S	272_V	0.797	0.19
130_V	185_A	0.796	0.19
286_Q	298_V	0.795	0.19
307_I	324_S	0.794	0.19
167_L	187_L	0.793	0.19
171_Q	326_A	0.789	0.18
30_A	82_C	0.785	0.18
265_G	328_M	0.784	0.18
278_A	285_P	0.784	0.18
245_G	334_S	0.781	0.18
34_Q	239_A	0.781	0.18
129_A	347_A	0.778	0.18
95_A	201_T	0.770	0.18
163_G	268_G	0.768	0.18
231_V	260_V	0.766	0.18
106_N	324_S	0.766	0.18
18_A	39_Q	0.765	0.18
24_W	29_A	0.762	0.18
275_S	324_S	0.762	0.18
127_P	164_S	0.762	0.18
205_P	235_D	0.761	0.18
113_Y	116_A	0.761	0.18
213_G	345_F	0.759	0.18
238_A	264_T	0.759	0.18
42_P	56_E	0.757	0.18
56_E	139_G	0.756	0.17
290_T	297_V	0.754	0.17
56_E	328_M	0.753	0.17
97_D	111_N	0.750	0.17
161_N	304_N	0.749	0.17
222_S	261_R	0.748	0.17
228_T	327_R	0.747	0.17
33_G	76_Y	0.747	0.17
157_G	215_I	0.746	0.17
20_V	71_N	0.744	0.17
107_W	188_Y	0.736	0.17
180_P	340_P	0.735	0.17
85_D	135_V	0.735	0.17
186_R	227_E	0.734	0.17
168_K	182_M	0.734	0.17
35_C	241_F	0.733	0.17
36_H	73_S	0.732	0.17
219_G	336_T	0.729	0.17
201_T	278_A	0.729	0.17
76_Y	337_G	0.726	0.17
72_E	80_C	0.725	0.17
121_Q	161_N	0.724	0.17
18_A	92_W	0.717	0.16
104_G	347_A	0.715	0.16
244_V	346_E	0.714	0.16
172_P	234_G	0.711	0.16
218_L	223_V	0.711	0.16
43_Y	339_P	0.709	0.16
78_V	157_G	0.709	0.16
25_C	71_N	0.707	0.16
70_W	170_L	0.703	0.16
243_V	253_T	0.701	0.16
35_C	340_P	0.700	0.16
241_F	340_P	0.699	0.16
246_H	330_V	0.698	0.16
61_G	295_V	0.698	0.16
192_N	295_V	0.695	0.16
54_E	184_L	0.694	0.16
261_R	330_V	0.694	0.16
259_P	328_M	0.692	0.16
328_M	351_V	0.691	0.16
73_S	272_V	0.691	0.16
222_S	241_F	0.690	0.16
214_T	291_S	0.685	0.16
33_G	82_C	0.680	0.16
109_K	333_V	0.679	0.16
245_G	309_P	0.677	0.15
56_E	294_G	0.676	0.15
160_G	187_L	0.674	0.15
32_S	81_D	0.673	0.15
301_D	336_T	0.665	0.15
18_A	23_L	0.662	0.15
116_A	209_Y	0.661	0.15
38_T	64_K	0.660	0.15
172_P	260_V	0.654	0.15
275_S	355_F	0.652	0.15
116_A	168_K	0.650	0.15
24_W	30_A	0.650	0.15
113_Y	216_N	0.650	0.15
50_V	63_V	0.641	0.15
54_E	57_A	0.640	0.15
246_H	331_Y	0.640	0.15
60_A	154_G	0.640	0.15
219_G	264_T	0.638	0.15
217_S	349_A	0.637	0.15
82_C	231_V	0.637	0.15
281_D	335_T	0.637	0.15
207_L	316_L	0.635	0.14
78_V	299_V	0.635	0.14
96_A	99_P	0.635	0.14
109_K	276_L	0.634	0.14
200_T	329_N	0.634	0.14
41_N	106_N	0.634	0.14
310_A	334_S	0.633	0.14
262_C	343_G	0.630	0.14
312_G	328_M	0.629	0.14
85_D	184_L	0.624	0.14
134_N	199_C	0.624	0.14
110_I	161_N	0.622	0.14
110_I	316_L	0.621	0.14
234_G	345_F	0.620	0.14
281_D	307_I	0.620	0.14
58_N	340_P	0.620	0.14
28_T	99_P	0.620	0.14
260_V	267_A	0.620	0.14
248_P	335_T	0.620	0.14
104_G	227_E	0.618	0.14
247_K	350_T	0.618	0.14
174_V	225_A	0.617	0.14
33_G	276_L	0.615	0.14
288_I	334_S	0.615	0.14
72_E	207_L	0.612	0.14
278_A	296_G	0.611	0.14
107_W	317_S	0.610	0.14
184_L	295_V	0.609	0.14
126_S	258_I	0.609	0.14
22_M	97_D	0.609	0.14
276_L	288_I	0.609	0.14
133_E	318_I	0.608	0.14
96_A	100_L	0.608	0.14
69_Q	84_K	0.607	0.14
159_S	207_L	0.600	0.14
78_V	206_V	0.599	0.14
335_T	348_T	0.599	0.14
45_G	48_F	0.599	0.14
56_E	232_D	0.597	0.14
277_T	289_K	0.594	0.14
37_T	160_G	0.594	0.14
201_T	206_V	0.593	0.14
263_N	286_Q	0.592	0.14
113_Y	337_G	0.592	0.14
248_P	333_V	0.592	0.14
132_F	136_G	0.591	0.14
179_I	315_P	0.591	0.14
178_V	214_T	0.591	0.14
56_E	127_P	0.590	0.13
86_N	148_P	0.590	0.13
173_F	334_S	0.589	0.13
275_S	297_V	0.589	0.13
93_A	100_L	0.588	0.13
188_Y	236_V	0.588	0.13
174_V	309_P	0.587	0.13
281_D	331_Y	0.586	0.13
286_Q	306_I	0.582	0.13
116_A	206_V	0.581	0.13
265_G	270_V	0.581	0.13
159_S	282_P	0.577	0.13
80_C	176_S	0.577	0.13
110_I	258_I	0.577	0.13
173_F	222_S	0.575	0.13
31_Y	39_Q	0.569	0.13
241_F	326_A	0.568	0.13
213_G	219_G	0.567	0.13
119_L	288_I	0.566	0.13
175_G	180_P	0.565	0.13
134_N	201_T	0.565	0.13
267_A	291_S	0.565	0.13
82_C	167_L	0.564	0.13
248_P	345_F	0.564	0.13
117_K	215_I	0.564	0.13
260_V	347_A	0.564	0.13
270_V	300_T	0.564	0.13
77_Y	210_Y	0.562	0.13
97_D	199_C	0.561	0.13
120_L	185_A	0.561	0.13
79_S	147_D	0.561	0.13
23_L	301_D	0.560	0.13
316_L	351_V	0.560	0.13
245_G	292_R	0.560	0.13
226_G	238_A	0.559	0.13
240_N	355_F	0.558	0.13
255_E	353_I	0.558	0.13
131_P	245_G	0.555	0.13
287_A	298_V	0.554	0.13
28_T	198_S	0.551	0.13
322_A	347_A	0.550	0.13
56_E	169_I	0.547	0.13
251_A	316_L	0.546	0.13
165_F	346_E	0.546	0.13
283_S	326_A	0.545	0.13
224_N	254_A	0.544	0.13
294_G	337_G	0.543	0.12
102_Y	105_D	0.542	0.12
60_A	172_P	0.540	0.12
127_P	132_F	0.538	0.12
179_I	248_P	0.537	0.12
275_S	352_R	0.537	0.12
95_A	206_V	0.536	0.12
161_N	209_Y	0.536	0.12
189_E	233_L	0.535	0.12
25_C	39_Q	0.529	0.12
172_P	175_G	0.529	0.12
278_A	351_V	0.529	0.12
217_S	230_E	0.529	0.12
61_G	292_R	0.524	0.12
200_T	259_P	0.524	0.12
231_V	254_A	0.524	0.12
21_L	125_S	0.522	0.12
94_F	121_Q	0.521	0.12
292_R	334_S	0.519	0.12
313_T	355_F	0.515	0.12
309_P	352_R	0.514	0.12
239_A	258_I	0.514	0.12
115_A	229_I	0.514	0.12
316_L	324_S	0.513	0.12
93_A	187_L	0.512	0.12
43_Y	208_V	0.512	0.12
61_G	111_N	0.511	0.12
325_I	349_A	0.511	0.12
219_G	294_G	0.510	0.12
335_T	352_R	0.509	0.12
278_A	301_D	0.507	0.12
215_I	323_D	0.505	0.12
307_I	331_Y	0.503	0.12
221_C	296_G	0.502	0.12
176_S	214_T	0.502	0.12
76_Y	207_L	0.502	0.12
116_A	160_G	0.501	0.12
183_A	186_R	0.501	0.12
175_G	241_F	0.500	0.12
135_V	141_T	0.500	0.12

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
5afoA	1	0.8848	100	0.503	Contact Map
1n12A	1	0.3511	99.8	0.808	Contact Map
2w07B	1	0.3315	99.7	0.817	Contact Map
1pdkB	1	0.382	99.7	0.82	Contact Map
2jmrA	1	0.4073	99.7	0.823	Contact Map
2xg5B	1	0.3848	99.7	0.825	Contact Map
2jtyA	1	0.4157	99.7	0.825	Contact Map
3jwnH	1	0.7528	99.7	0.828	Contact Map
3bwuF	1	0.3315	99.7	0.831	Contact Map
2uy6B	2	0.4045	99.6	0.832	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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