GREMLIN Database
PFLE - Putative pyruvate formate-lyase 3-activating enzyme
UniProt: P75794 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13470
Length: 299 (290)
Sequences: 919 (771)
Seq/√Len: 45.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
129_L141_A5.2511.00
187_D197_N5.0051.00
3_F37_S4.2091.00
2_I22_L4.1601.00
127_L148_S3.1091.00
165_W207_K3.0681.00
161_L180_A3.0371.00
180_A205_L2.9991.00
154_H177_L2.9151.00
145_L155_T2.8841.00
113_V148_S2.8171.00
219_V248_I2.7921.00
114_L151_A2.7841.00
147_A150_E2.7741.00
82_P85_L2.7581.00
85_L88_L2.7051.00
82_P88_L2.6201.00
187_D199_A2.5961.00
146_Q174_Y2.5621.00
106_V147_A2.5611.00
6_Q9_S2.5021.00
285_Q289_C2.3151.00
142_M170_P2.2891.00
112_T115_R2.2821.00
189_P192_Q2.1821.00
104_K109_I2.1701.00
193_W225_F2.1361.00
33_Q158_E2.1351.00
110_M148_S2.1271.00
217_I239_A2.1241.00
149_H176_D2.1221.00
185_V238_F2.0991.00
27_L201_V2.0811.00
62_A88_L2.0490.99
190_F198_A2.0130.99
231_S235_I1.9870.99
191_K197_N1.9550.99
109_I144_L1.9480.99
20_V113_V1.9320.99
169_A207_K1.8240.99
64_E83_E1.8160.99
23_K43_D1.7950.98
158_E216_I1.7920.98
107_E147_A1.7630.98
165_W169_A1.7420.98
52_L94_T1.7350.98
62_A85_L1.7300.98
175_I178_F1.6950.98
147_A151_A1.6820.98
276_D281_L1.6520.97
109_I113_V1.6500.97
135_F163_V1.6290.97
26_S160_C1.6240.97
117_K121_D1.6050.97
129_L144_L1.6010.97
67_E75_I1.5990.97
165_W203_D1.5980.97
206_K241_D1.5950.97
178_F208_L1.5880.97
216_I247_E1.5870.97
190_F196_G1.5730.96
229_E233_K1.5670.96
77_R97_L1.5580.96
43_D266_L1.5430.96
236_T288_A1.5430.96
184_H225_F1.5160.96
62_A82_P1.5140.96
19_V130_S1.5090.96
135_F171_S1.5080.96
202_L238_F1.5080.96
19_V158_E1.4800.95
229_E283_F1.4760.95
16_I120_Y1.4750.95
221_L250_F1.4740.95
129_L134_P1.4700.95
22_L134_P1.4330.94
237_D291_K1.4100.94
87_A90_D1.4040.93
78_E81_T1.3680.93
162_H196_G1.3220.91
206_K238_F1.3180.91
40_R136_M1.3170.91
219_V235_I1.3170.91
261_Y268_Y1.3120.91
276_D280_L1.3100.91
135_F168_I1.3080.91
226_N231_S1.2970.90
223_Q273_K1.2780.90
203_D207_K1.2690.89
48_A262_H1.2650.89
254_H257_G1.2510.89
142_M146_Q1.2450.88
18_T116_D1.2320.88
286_Q290_Q1.2280.88
129_L145_L1.2200.87
63_P87_A1.2150.87
134_P141_A1.2120.87
30_R193_W1.2110.87
161_L205_L1.2040.87
14_P177_L1.2000.87
38_R266_L1.1990.86
226_N254_H1.1990.86
157_V178_F1.1990.86
30_R192_Q1.1920.86
102_E108_E1.1910.86
106_V140_M1.1820.86
7_R115_R1.1760.85
185_V231_S1.1700.85
179_L252_P1.1430.84
47_D263_L1.1330.83
38_R261_Y1.1260.83
280_L284_A1.1140.82
110_M151_A1.1100.82
34_N260_K1.0950.81
148_S155_T1.0860.80
257_G268_Y1.0830.80
26_S162_H1.0820.80
26_S136_M1.0760.80
184_H193_W1.0760.80
110_M147_A1.0650.79
66_I88_L1.0580.79
178_F213_K1.0540.78
114_L117_K1.0500.78
230_T234_A1.0420.78
216_I249_H1.0390.77
206_K210_A1.0270.77
30_R269_D1.0260.76
185_V234_A1.0230.76
162_H197_N1.0220.76
57_L93_P1.0200.76
135_F157_V1.0100.75
182_L202_L1.0010.75
44_L97_L0.9940.74
178_F205_L0.9940.74
56_E59_A0.9940.74
66_I85_L0.9890.74
189_P228_D0.9880.74
138_P167_Y0.9880.74
149_H174_Y0.9880.74
105_S108_E0.9820.73
33_Q38_R0.9780.73
221_L281_L0.9650.72
117_K151_A0.9630.72
70_L73_L0.9620.72
228_D231_S0.9600.72
135_F160_C0.9470.71
22_L129_L0.9470.71
145_L175_I0.9450.70
66_I81_T0.9440.70
106_V144_L0.9440.70
116_D120_Y0.9390.70
156_A177_L0.9350.70
158_E256_L0.9320.69
149_H155_T0.9300.69
158_E179_L0.9290.69
196_G201_V0.9160.68
78_E139_E0.9090.67
185_V235_I0.9090.67
107_E111_T0.9070.67
33_Q257_G0.9060.67
239_A248_I0.9010.67
7_R116_D0.8940.66
189_P230_T0.8920.66
287_Y291_K0.8910.66
127_L153_I0.8910.66
162_H200_R0.8870.65
232_V288_A0.8850.65
45_L100_C0.8840.65
187_D191_K0.8780.65
190_F201_V0.8750.64
4_N102_E0.8670.64
22_L141_A0.8640.63
226_N257_G0.8640.63
14_P154_H0.8570.63
5_I112_T0.8560.63
56_E69_A0.8530.62
198_A202_L0.8520.62
142_M171_S0.8470.62
184_H231_S0.8470.62
141_A145_L0.8460.62
179_L249_H0.8440.62
59_A69_A0.8410.61
209_A243_L0.8390.61
139_E170_P0.8370.61
23_K101_G0.8320.60
19_V33_Q0.8290.60
6_Q257_G0.8280.60
109_I140_M0.8220.59
205_L217_I0.8220.59
163_V168_I0.8220.59
158_E249_H0.8170.59
157_V163_V0.8150.59
209_A215_I0.8140.59
203_D206_K0.8120.59
142_M174_Y0.8110.58
7_R120_Y0.8080.58
198_A201_V0.8070.58
205_L238_F0.8050.58
233_K237_D0.8030.58
203_D210_A0.8030.58
134_P157_V0.8010.57
184_H226_N0.8010.57
127_L144_L0.8000.57
259_N263_L0.7930.57
51_C92_C0.7920.57
135_F141_A0.7890.56
227_A280_L0.7890.56
175_I205_L0.7870.56
166_K169_A0.7840.56
224_G227_A0.7790.55
164_P200_R0.7780.55
209_A242_E0.7770.55
61_A91_C0.7730.55
186_A234_A0.7730.55
192_Q225_F0.7680.54
33_Q208_L0.7680.54
182_L205_L0.7640.54
111_T237_D0.7610.54
236_T293_L0.7610.54
133_E160_C0.7530.53
281_L284_A0.7510.53
7_R134_P0.7460.52
207_K211_A0.7460.52
4_N23_K0.7450.52
199_A203_D0.7400.52
267_P271_P0.7380.51
113_V144_L0.7380.51
75_I119_F0.7350.51
68_R81_T0.7350.51
229_E280_L0.7320.51
31_W220_P0.7320.51
59_A65_V0.7320.51
137_Q173_P0.7320.51
45_L263_L0.7310.51
28_G38_R0.7280.50
227_A284_A0.7270.50
136_M160_C0.7270.50
280_L283_F0.7250.50
159_T178_F0.7240.50
7_R23_K0.7210.50
22_L104_K0.7170.49
16_I253_Y0.7170.49
253_Y270_A0.7160.49
219_V231_S0.7160.49
3_F23_K0.7160.49
33_Q256_L0.7160.49
2_I104_K0.7140.49
19_V122_R0.7090.49
106_V143_A0.7090.49
232_V235_I0.7060.48
261_Y264_L0.7040.48
98_T263_L0.7030.48
56_E83_E0.6950.47
39_A43_D0.6940.47
185_V202_L0.6940.47
282_D286_Q0.6940.47
161_L168_I0.6930.47
205_L215_I0.6920.47
236_T291_K0.6920.47
189_P231_S0.6890.47
114_L153_I0.6890.47
13_G17_R0.6840.46
86_T89_T0.6830.46
135_F167_Y0.6820.46
221_L225_F0.6820.46
6_Q19_V0.6820.46
158_E175_I0.6800.46
2_I20_V0.6790.46
42_Q103_V0.6780.46
83_E87_A0.6780.46
22_L109_I0.6780.46
9_S37_S0.6710.45
75_I153_I0.6660.44
26_S231_S0.6640.44
20_V109_I0.6640.44
178_F215_I0.6630.44
25_C29_C0.6620.44
113_V127_L0.6590.44
258_I272_E0.6580.44
282_D285_Q0.6540.43
82_P86_T0.6480.43
57_L91_C0.6420.42
250_F253_Y0.6420.42
38_R43_D0.6400.42
46_Y75_I0.6390.42
28_G60_K0.6390.42
219_V239_A0.6370.42
284_A287_Y0.6360.42
51_C58_C0.6350.41
160_C163_V0.6350.41
96_A101_G0.6340.41
59_A111_T0.6330.41
140_M190_F0.6320.41
59_A67_E0.6300.41
31_W36_E0.6280.41
157_V192_Q0.6250.40
50_L96_A0.6250.40
158_E180_A0.6250.40
14_P119_F0.6230.40
28_G108_E0.6220.40
6_Q34_N0.6210.40
102_E112_T0.6190.40
261_Y266_L0.6170.40
217_I238_F0.6150.40
51_C55_C0.6130.39
201_V253_Y0.6130.39
249_H263_L0.6090.39
114_L148_S0.6090.39
10_T225_F0.6080.39
66_I78_E0.6030.38
156_A179_L0.6030.38
72_G91_C0.6030.38
6_Q260_K0.5990.38
12_D17_R0.5980.38
154_H176_D0.5970.38
158_E263_L0.5950.38
56_E195_D0.5950.38
169_A173_P0.5940.38
108_E135_F0.5940.38
4_N231_S0.5930.38
133_E159_T0.5930.38
101_G212_G0.5930.38
248_I254_H0.5930.38
36_E260_K0.5910.37
16_I23_K0.5890.37
8_Y151_A0.5890.37
104_K113_V0.5890.37
159_T180_A0.5890.37
184_H189_P0.5880.37
48_A94_T0.5880.37
219_V223_Q0.5870.37
109_I148_S0.5860.37
277_A281_L0.5860.37
26_S163_V0.5850.37
114_L150_E0.5840.37
95_Q119_F0.5840.37
146_Q150_E0.5830.37
137_Q155_T0.5810.36
241_D267_P0.5800.36
29_C32_C0.5780.36
106_V113_V0.5780.36
159_T254_H0.5770.36
4_N7_R0.5760.36
27_L162_H0.5760.36
113_V129_L0.5730.36
217_I261_Y0.5710.36
120_Y123_S0.5690.35
61_A188_A0.5670.35
28_G194_T0.5670.35
38_R160_C0.5660.35
145_L262_H0.5660.35
73_L76_H0.5650.35
108_E144_L0.5630.35
172_L207_K0.5620.35
101_G116_D0.5620.35
55_C58_C0.5620.35
165_W211_A0.5610.35
191_K282_D0.5600.35
172_L213_K0.5590.34
9_S50_L0.5570.34
268_Y273_K0.5560.34
277_A280_L0.5550.34
4_N154_H0.5550.34
169_A172_L0.5520.34
169_A211_A0.5520.34
136_M143_A0.5520.34
12_D15_G0.5520.34
27_L160_C0.5510.34
46_Y54_G0.5510.34
162_H201_V0.5480.33
47_D149_H0.5480.33
91_C239_A0.5470.33
6_Q120_Y0.5470.33
3_F45_L0.5470.33
109_I141_A0.5460.33
27_L226_N0.5450.33
37_S46_Y0.5450.33
188_A230_T0.5440.33
272_E278_P0.5440.33
110_M144_L0.5430.33
110_M114_L0.5420.33
191_K236_T0.5400.33
232_V284_A0.5390.33
33_Q254_H0.5390.33
231_S236_T0.5390.33
33_Q270_A0.5370.33
12_D25_C0.5360.32
160_C180_A0.5350.32
3_F155_T0.5350.32
140_M223_Q0.5350.32
89_T97_L0.5330.32
100_C263_L0.5320.32
82_P161_L0.5320.32
205_L208_L0.5320.32
99_V293_L0.5300.32
200_R224_G0.5280.32
57_L60_K0.5280.32
254_H259_N0.5260.32
30_R225_F0.5260.32
220_P251_L0.5250.32
10_T288_A0.5230.31
60_K102_E0.5210.31
142_M173_P0.5200.31
19_V141_A0.5200.31
251_L254_H0.5190.31
84_H134_P0.5180.31
4_N37_S0.5170.31
270_A276_D0.5170.31
31_W251_L0.5170.31
116_D260_K0.5170.31
146_Q173_P0.5160.31
228_D279_E0.5150.31
272_E281_L0.5140.31
224_G290_Q0.5130.31
38_R158_E0.5120.30
290_Q293_L0.5120.30
250_F284_A0.5110.30
278_P290_Q0.5110.30
158_E268_Y0.5100.30
120_Y124_G0.5090.30
189_P225_F0.5090.30
142_M149_H0.5090.30
221_L227_A0.5070.30
214_K246_G0.5070.30
134_P163_V0.5070.30
152_G250_F0.5060.30
72_G212_G0.5060.30
40_R104_K0.5050.30
239_A293_L0.5040.30
219_V284_A0.5030.30
150_E164_P0.5020.30
2_I69_A0.5020.30
219_V288_A0.5010.30
66_I82_P0.5010.30
36_E204_N0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3c8fA 1 0.7993 100 0.639 Contact Map
1hfeL 2 0.8629 100 0.704 Contact Map
3canA 2 0.5284 100 0.71 Contact Map
3rfaA 1 0.7391 100 0.768 Contact Map
3c8yA 1 0.8963 100 0.777 Contact Map
1q16B 2 0.8428 100 0.783 Contact Map
2z2uA 1 0.6689 99.9 0.791 Contact Map
2yx0A 1 0.7559 99.9 0.792 Contact Map
2vpzB 2 0.5786 99.9 0.802 Contact Map
4r0vA 1 0.5987 99.9 0.804 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0093 seconds.