WCAL - ECOLI - GREMLIN Contacts

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WCAL - Putative colanic acid biosynthesis glycosyltransferase WcaL

UniProt:	P71243	Sequences: MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQNTHAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESRNLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREVLNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKAPATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQLEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVSTLHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQQVINRELASLLQAL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG12652
Length:	406 (353)
Sequences:	48465 (40577)
Seq/√Len:	2159.7

ECOLI - WCAL RFAG GLGA RFAB WBBK WCAI WCAC
BACSU - YPJH GLGA YQGM COTSA EPSF YTCC EPSD TUAC TUAH YKVP CGEB TAGE
HALSA - Q9HQQ0 Q9HSW1 Q9HS45 Q9HSV4 Q9HRP8 Q9HSV2 Q9HQT2 Q9HRP9
SULSO - Q980C8 Q97XT7 Q97UM0 Q97V61 Q97ZD3 Q97V80 Q97VL4 Q97XU2 Q97XT8 Q7LXI2 Q97UU3 Q97XT9 Q97XT5 Q7LXM9 Q7LXF8 Q97XT1 Q97XT6 Q97XF6 Q7LXF5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
226_I	299_A	4.631	1.00
255_R	282_E	3.695	1.00
177_L	200_A	3.156	1.00
302_F	324_A	3.087	1.00
123_I	403_L	2.948	1.00
224_E	257_R	2.888	1.00
222_P	255_R	2.713	1.00
385_K	389_D	2.688	1.00
257_R	284_P	2.670	1.00
265_E	269_R	2.640	1.00
242_E	271_L	2.638	1.00
246_Q	361_A	2.636	1.00
250_Q	365_A	2.612	1.00
227_S	237_L	2.552	1.00
248_K	280_V	2.367	1.00
186_A	190_Q	2.276	1.00
242_E	357_A	2.261	1.00
223_L	368_S	2.211	1.00
33_E	119_A	2.173	1.00
236_G	239_V	2.147	1.00
33_E	117_F	2.127	1.00
246_Q	358_R	2.122	1.00
371_D	374_E	2.060	1.00
335_L	340_P	2.058	1.00
244_C	256_Y	2.057	1.00
259_L	295_M	2.052	1.00
271_L	275_Y	2.049	1.00
363_R	366_A	1.982	1.00
224_E	255_R	1.976	1.00
30_F	400_A	1.932	1.00
257_R	282_E	1.890	1.00
343_V	349_G	1.883	1.00
224_E	298_D	1.871	1.00
225_I	301_V	1.870	1.00
348_S	385_K	1.869	1.00
335_L	353_P	1.856	1.00
123_I	149_I	1.850	1.00
328_G	383_R	1.850	1.00
344_E	347_K	1.828	1.00
366_A	374_E	1.827	1.00
375_L	379_V	1.820	1.00
264_W	267_R	1.818	1.00
236_G	307_V	1.799	1.00
394_V	398_E	1.795	1.00
223_L	300_D	1.789	1.00
183_D	186_A	1.786	1.00
353_P	359_A	1.765	1.00
4_G	119_A	1.765	1.00
28_M	397_R	1.755	1.00
261_I	265_E	1.750	1.00
350_W	363_R	1.745	1.00
245_R	275_Y	1.730	1.00
205_G	392_Q	1.702	1.00
302_F	329_I	1.696	1.00
366_A	371_D	1.683	1.00
186_A	201_V	1.682	1.00
273_E	278_E	1.675	1.00
148_T	175_G	1.672	1.00
240_A	256_Y	1.658	1.00
228_V	292_V	1.628	1.00
365_A	369_Q	1.628	1.00
287_K	291_E	1.623	1.00
183_D	341_E	1.614	1.00
347_K	381_R	1.600	1.00
177_L	402_L	1.600	1.00
244_C	254_F	1.587	1.00
180_P	199_I	1.560	1.00
4_G	35_L	1.552	1.00
374_E	377_P	1.536	1.00
356_D	359_A	1.532	1.00
240_A	303_L	1.526	1.00
247_L	365_A	1.525	1.00
24_A	27_D	1.517	1.00
24_A	393_Q	1.505	1.00
359_A	363_R	1.502	1.00
350_W	366_A	1.498	1.00
186_A	199_I	1.492	1.00
344_E	385_K	1.490	1.00
291_E	294_A	1.488	1.00
384_E	388_H	1.474	1.00
344_E	348_S	1.474	1.00
24_A	396_N	1.470	1.00
377_P	381_R	1.446	1.00
19_L	23_T	1.445	1.00
288_P	291_E	1.436	1.00
183_D	201_V	1.427	1.00
28_M	400_A	1.424	1.00
259_L	284_P	1.417	1.00
234_K	314_M	1.416	1.00
287_K	295_M	1.377	1.00
239_V	307_V	1.369	1.00
239_V	357_A	1.361	1.00
238_H	271_L	1.356	1.00
333_S	339_I	1.352	1.00
357_A	361_A	1.350	1.00
225_I	364_L	1.343	1.00
5_F	25_F	1.341	1.00
33_E	61_L	1.337	1.00
224_E	300_D	1.334	1.00
243_A	360_L	1.332	1.00
270_T	274_Q	1.321	1.00
381_R	384_E	1.318	1.00
231_L	258_I	1.318	1.00
219_P	222_P	1.317	1.00
299_A	329_I	1.310	1.00
259_L	287_K	1.307	1.00
352_V	363_R	1.302	1.00
257_R	295_M	1.301	1.00
124_A	146_I	1.299	1.00
179_L	399_L	1.279	1.00
225_I	303_L	1.269	1.00
284_P	287_K	1.266	1.00
271_L	274_Q	1.266	1.00
246_Q	357_A	1.262	1.00
398_E	401_S	1.254	1.00
237_L	258_I	1.250	1.00
183_D	187_G	1.238	1.00
180_P	186_A	1.237	1.00
227_S	240_A	1.225	1.00
394_V	397_R	1.220	1.00
211_F	327_V	1.213	1.00
359_A	362_Q	1.213	1.00
226_I	259_L	1.208	1.00
223_L	301_V	1.202	1.00
5_F	125_H	1.198	1.00
376_A	380_K	1.194	1.00
266_R	269_R	1.190	1.00
257_R	299_A	1.185	1.00
293_K	327_V	1.181	1.00
269_R	273_E	1.179	1.00
33_E	59_R	1.179	1.00
266_R	270_T	1.176	1.00
317_I	322_M	1.175	1.00
306_S	315_E	1.164	1.00
187_G	190_Q	1.164	1.00
179_L	402_L	1.159	1.00
26_I	29_G	1.156	1.00
304_L	321_L	1.155	1.00
245_R	249_E	1.148	1.00
321_L	339_I	1.146	1.00
17_F	319_V	1.142	1.00
345_A	363_R	1.133	1.00
151_H	319_V	1.123	1.00
307_V	355_N	1.119	1.00
292_V	296_L	1.115	1.00
372_T	375_L	1.113	1.00
5_F	22_I	1.111	1.00
223_L	254_F	1.108	1.00
210_R	293_K	1.101	1.00
333_S	351_L	1.100	1.00
272_I	283_M	1.099	1.00
379_V	382_A	1.097	1.00
361_A	365_A	1.090	1.00
190_Q	196_R	1.088	1.00
297_D	327_V	1.086	1.00
328_G	380_K	1.082	1.00
24_A	28_M	1.081	1.00
391_N	394_V	1.080	1.00
9_K	12_L	1.077	1.00
239_V	334_T	1.072	1.00
224_E	299_A	1.066	1.00
261_I	286_F	1.066	1.00
238_H	307_V	1.064	1.00
222_P	253_A	1.061	1.00
367_F	378_V	1.060	1.00
334_T	352_V	1.058	1.00
305_P	360_L	1.057	1.00
230_R	234_K	1.056	1.00
296_L	327_V	1.055	1.00
181_I	185_W	1.054	1.00
304_L	320_A	1.050	1.00
246_Q	250_Q	1.048	1.00
246_Q	249_E	1.047	1.00
180_P	189_L	1.039	1.00
337_S	340_P	1.035	1.00
350_W	378_V	1.032	1.00
238_H	242_E	1.029	1.00
329_I	379_V	1.025	1.00
392_Q	395_I	1.019	1.00
223_L	253_A	1.019	1.00
211_F	293_K	1.015	1.00
395_I	398_E	1.014	1.00
239_V	355_N	1.013	1.00
374_E	378_V	1.010	1.00
302_F	320_A	1.010	1.00
247_L	254_F	1.004	1.00
350_W	367_F	1.002	1.00
22_I	34_I	0.999	1.00
362_Q	366_A	0.997	1.00
187_G	191_K	0.994	1.00
181_I	204_M	0.993	1.00
284_P	295_M	0.992	1.00
257_R	298_D	0.989	1.00
226_I	295_M	0.986	1.00
318_P	339_I	0.980	1.00
228_V	259_L	0.980	1.00
181_I	316_G	0.978	1.00
228_V	296_L	0.972	1.00
108_A	112_Q	0.972	1.00
315_E	318_P	0.971	1.00
195_P	198_K	0.969	1.00
247_L	364_L	0.969	1.00
289_S	292_V	0.969	1.00
239_V	242_E	0.969	1.00
325_M	331_V	0.967	1.00
372_T	376_A	0.964	1.00
3_V	30_F	0.962	1.00
124_A	148_T	0.961	1.00
111_G	139_L	0.955	1.00
352_V	360_L	0.950	1.00
272_I	277_L	0.947	1.00
241_I	272_I	0.947	1.00
146_I	175_G	0.946	1.00
338_G	341_E	0.943	1.00
225_I	244_C	0.940	1.00
180_P	185_W	0.935	1.00
325_M	382_A	0.934	1.00
235_K	315_E	0.934	1.00
293_K	297_D	0.931	1.00
380_K	384_E	0.928	1.00
333_S	343_V	0.928	1.00
238_H	267_R	0.927	1.00
20_N	392_Q	0.925	1.00
242_E	275_Y	0.923	1.00
17_F	151_H	0.918	1.00
395_I	399_L	0.915	1.00
397_R	401_S	0.913	1.00
324_A	329_I	0.911	1.00
267_R	270_T	0.908	1.00
254_F	368_S	0.907	1.00
125_H	151_H	0.906	1.00
353_P	356_D	0.904	1.00
113_V	117_F	0.903	1.00
244_C	280_V	0.902	1.00
208_M	391_N	0.901	1.00
204_M	319_V	0.898	1.00
379_V	383_R	0.897	1.00
232_T	263_P	0.896	1.00
188_R	191_K	0.893	1.00
247_L	368_S	0.888	1.00
332_V	352_V	0.888	1.00
20_N	23_T	0.887	1.00
245_R	248_K	0.887	1.00
207_D	210_R	0.886	1.00
358_R	362_Q	0.883	1.00
205_G	395_I	0.882	1.00
301_V	367_F	0.882	1.00
378_V	381_R	0.876	1.00
321_L	342_L	0.874	1.00
240_A	305_P	0.871	1.00
350_W	374_E	0.870	1.00
267_R	271_L	0.869	1.00
321_L	331_V	0.869	1.00
348_S	382_A	0.868	1.00
376_A	379_V	0.868	1.00
239_V	360_L	0.858	1.00
330_P	379_V	0.857	1.00
226_I	296_L	0.857	1.00
175_G	178_M	0.857	1.00
270_T	273_E	0.856	1.00
207_D	211_F	0.854	1.00
22_I	26_I	0.854	1.00
243_A	361_A	0.853	1.00
202_S	402_L	0.852	1.00
227_S	258_I	0.849	1.00
147_A	179_L	0.848	1.00
16_T	20_N	0.841	1.00
229_A	237_L	0.839	1.00
303_L	364_L	0.838	1.00
385_K	390_F	0.834	1.00
362_Q	365_A	0.830	1.00
227_S	305_P	0.826	1.00
321_L	333_S	0.824	1.00
391_N	395_I	0.821	1.00
304_L	339_I	0.820	1.00
26_I	32_V	0.819	1.00
332_V	363_R	0.817	1.00
247_L	361_A	0.817	1.00
272_I	281_V	0.816	1.00
248_K	252_V	0.813	1.00
301_V	364_L	0.812	1.00
336_H	339_I	0.805	1.00
184_L	187_G	0.804	1.00
227_S	302_F	0.803	1.00
203_R	341_E	0.802	1.00
170_Q	174_R	0.802	1.00
330_P	367_F	0.802	1.00
200_A	398_E	0.801	1.00
200_A	402_L	0.800	1.00
17_F	149_I	0.795	1.00
247_L	252_V	0.795	1.00
296_L	329_I	0.794	1.00
255_R	280_V	0.793	1.00
179_L	202_S	0.792	1.00
152_G	234_K	0.791	1.00
203_R	390_F	0.789	1.00
295_M	298_D	0.788	1.00
235_K	320_A	0.786	1.00
219_P	255_R	0.779	1.00
250_Q	362_Q	0.774	1.00
345_A	348_S	0.774	1.00
325_M	348_S	0.767	1.00
318_P	321_L	0.766	1.00
121_V	403_L	0.764	1.00
242_E	246_Q	0.764	1.00
243_A	357_A	0.763	1.00
17_F	204_M	0.762	1.00
151_H	181_I	0.761	1.00
20_N	207_D	0.761	1.00
233_E	238_H	0.759	1.00
190_Q	199_I	0.758	1.00
7_L	18_V	0.756	1.00
397_R	400_A	0.753	1.00
294_A	297_D	0.753	1.00
343_V	351_L	0.753	1.00
18_V	125_H	0.752	1.00
145_K	176_D	0.752	1.00
21_Q	399_L	0.751	1.00
392_Q	396_N	0.750	1.00
242_E	245_R	0.749	1.00
214_R	297_D	0.748	1.00
28_M	393_Q	0.747	1.00
125_H	149_I	0.743	1.00
104_L	108_A	0.743	1.00
20_N	24_A	0.742	1.00
228_V	302_F	0.740	1.00
235_K	306_S	0.740	1.00
250_Q	361_A	0.740	1.00
181_I	319_V	0.737	1.00
294_A	298_D	0.736	1.00
245_R	277_L	0.735	1.00
325_M	385_K	0.734	1.00
335_L	351_L	0.730	1.00
393_Q	397_R	0.727	1.00
203_R	398_E	0.725	1.00
345_A	350_W	0.722	1.00
5_F	123_I	0.722	1.00
272_I	275_Y	0.721	1.00
332_V	367_F	0.721	1.00
225_I	254_F	0.720	1.00
124_A	130_G	0.720	1.00
124_A	133_A	0.717	1.00
137_R	146_I	0.716	1.00
250_Q	369_Q	0.714	1.00
227_S	256_Y	0.710	1.00
366_A	369_Q	0.710	1.00
289_S	293_K	0.708	1.00
317_I	390_F	0.706	1.00
57_R	63_D	0.704	1.00
123_I	399_L	0.704	1.00
300_D	375_L	0.702	1.00
287_K	292_V	0.702	1.00
377_P	380_K	0.701	1.00
371_D	375_L	0.700	1.00
22_I	32_V	0.698	1.00
322_M	390_F	0.698	1.00
169_Q	173_R	0.698	1.00
58_T	61_L	0.697	1.00
260_G	285_G	0.696	1.00
147_A	177_L	0.696	1.00
304_L	318_P	0.693	1.00
220_A	223_L	0.692	1.00
231_L	265_E	0.692	1.00
378_V	382_A	0.686	1.00
16_T	289_S	0.685	1.00
121_V	147_A	0.684	1.00
184_L	188_R	0.684	1.00
233_E	307_V	0.683	1.00
325_M	379_V	0.681	1.00
223_L	372_T	0.679	1.00
269_R	283_M	0.678	1.00
28_M	396_N	0.677	1.00
399_L	403_L	0.674	1.00
373_D	377_P	0.671	1.00
23_T	56_A	0.670	1.00
208_M	326_A	0.669	1.00
225_I	368_S	0.669	1.00
334_T	360_L	0.668	1.00
57_R	62_Q	0.666	1.00
233_E	264_W	0.663	1.00
367_F	375_L	0.663	1.00
33_E	37_L	0.660	1.00
383_R	386_V	0.660	1.00
147_A	403_L	0.659	1.00
135_K	139_L	0.659	1.00
5_F	32_V	0.659	1.00
107_S	111_G	0.658	1.00
303_L	360_L	0.657	1.00
236_G	305_P	0.656	1.00
122_F	144_G	0.653	1.00
202_S	399_L	0.652	1.00
56_A	63_D	0.652	1.00
373_D	376_A	0.652	1.00
27_D	393_Q	0.650	1.00
268_L	283_M	0.649	1.00
200_A	203_R	0.649	1.00
358_R	361_A	0.643	1.00
296_L	320_A	0.639	1.00
17_F	399_L	0.639	1.00
111_G	115_T	0.636	1.00
340_P	351_L	0.635	1.00
262_G	265_E	0.634	1.00
334_T	354_E	0.633	1.00
223_L	226_I	0.631	1.00
352_V	359_A	0.629	1.00
348_S	381_R	0.629	1.00
166_P	169_Q	0.628	1.00
26_I	30_F	0.626	1.00
346_D	366_A	0.624	1.00
332_V	364_L	0.623	1.00
149_I	399_L	0.622	1.00
343_V	348_S	0.620	1.00
244_C	247_L	0.619	1.00
382_A	385_K	0.617	1.00
108_A	111_G	0.617	1.00
17_F	125_H	0.615	1.00
184_L	340_P	0.612	1.00
229_A	235_K	0.608	1.00
57_R	61_L	0.608	1.00
137_R	176_D	0.605	1.00
23_T	27_D	0.604	1.00
295_M	299_A	0.603	1.00
238_H	264_W	0.602	1.00
236_G	355_N	0.602	1.00
16_T	19_L	0.601	1.00
381_R	385_K	0.601	1.00
253_A	280_V	0.600	1.00
182_S	185_W	0.599	1.00
20_N	319_V	0.595	1.00
203_R	317_I	0.595	1.00
166_P	170_Q	0.595	1.00
142_I	145_K	0.594	1.00
210_R	289_S	0.592	1.00
151_H	204_M	0.590	1.00
241_I	281_V	0.590	1.00
4_G	122_F	0.590	1.00
183_D	203_R	0.588	1.00
100_E	103_N	0.588	1.00
238_H	268_L	0.587	1.00
302_F	331_V	0.584	1.00
29_G	400_A	0.581	1.00
243_A	364_L	0.579	1.00
252_V	369_Q	0.579	1.00
320_A	324_A	0.578	1.00
231_L	237_L	0.576	1.00
59_R	62_Q	0.576	1.00
162_N	165_T	0.576	1.00
224_E	295_M	0.576	1.00
114_A	119_A	0.575	1.00
318_P	322_M	0.575	1.00
202_S	398_E	0.573	1.00
115_T	142_I	0.572	1.00
113_V	116_P	0.571	1.00
186_A	196_R	0.569	1.00
221_T	253_A	0.569	1.00
162_N	166_P	0.569	1.00
230_R	235_K	0.568	1.00
256_Y	281_V	0.566	1.00
185_W	188_R	0.563	1.00
35_L	113_V	0.562	1.00
119_A	144_G	0.561	1.00
389_D	394_V	0.561	1.00
306_S	320_A	0.560	1.00
212_S	297_D	0.559	1.00
226_I	329_I	0.554	1.00
248_K	254_F	0.554	1.00
240_A	244_C	0.554	1.00
4_G	33_E	0.553	1.00
259_L	292_V	0.553	1.00
389_D	398_E	0.550	1.00
226_I	257_R	0.549	1.00
322_M	386_V	0.549	1.00
226_I	302_F	0.548	1.00
17_F	21_Q	0.548	1.00
26_I	57_R	0.547	1.00
301_V	332_V	0.547	1.00
112_Q	116_P	0.545	1.00
223_L	375_L	0.545	1.00
296_L	302_F	0.545	1.00
193_G	196_R	0.544	1.00
149_I	179_L	0.541	1.00
109_I	112_Q	0.540	1.00
209_T	212_S	0.539	1.00
23_T	26_I	0.538	1.00
325_M	386_V	0.538	1.00
31_E	59_R	0.535	1.00
24_A	392_Q	0.535	1.00
5_F	403_L	0.534	1.00
237_L	264_W	0.533	1.00
102_R	105_I	0.533	1.00
305_P	334_T	0.533	1.00
300_D	372_T	0.532	1.00
328_G	379_V	0.531	1.00
212_S	215_P	0.530	1.00
239_V	243_A	0.528	1.00
146_I	176_D	0.528	1.00
380_K	383_R	0.527	1.00
137_R	142_I	0.526	1.00
152_G	157_S	0.525	1.00
272_I	278_E	0.525	1.00
396_N	400_A	0.525	1.00
301_V	375_L	0.525	1.00
130_G	133_A	0.524	1.00
241_I	268_L	0.524	1.00
301_V	368_S	0.524	1.00
170_Q	173_R	0.523	1.00
241_I	271_L	0.523	1.00
102_R	106_L	0.522	1.00
163_H	166_P	0.522	1.00
269_R	272_I	0.522	1.00
180_P	201_V	0.521	1.00
324_A	331_V	0.519	1.00
375_L	378_V	0.518	1.00
315_E	320_A	0.518	1.00
154_D	157_S	0.518	1.00
59_R	117_F	0.518	1.00
138_E	174_R	0.516	1.00
29_G	32_V	0.514	1.00
365_A	368_S	0.508	1.00
259_L	299_A	0.508	1.00
230_R	320_A	0.507	1.00
336_H	354_E	0.507	1.00
292_V	295_M	0.507	1.00
322_M	342_L	0.506	1.00
268_L	271_L	0.505	1.00
261_I	285_G	0.504	1.00
185_W	316_G	0.504	1.00
121_V	145_K	0.502	1.00
243_A	303_L	0.500	1.00
330_P	375_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2r60A	1	0.9212	100	0.164	Contact Map
3c48A	2	0.8966	100	0.176	Contact Map
4n9wA	1	0.8424	100	0.178	Contact Map
1rzuA	1	0.9483	100	0.199	Contact Map
2x0dA	2	0.835	100	0.207	Contact Map
3froA	3	0.9261	100	0.207	Contact Map
3okpA	1	0.8719	100	0.208	Contact Map
3s28A	4	0.9335	100	0.208	Contact Map
2jjmA	4	0.8571	100	0.208	Contact Map
4rbnA	3	0.936	100	0.215	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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