GREMLIN Database
WCAL - Putative colanic acid biosynthesis glycosyltransferase WcaL
UniProt: P71243 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12652
Length: 406 (353)
Sequences: 48465 (40577)
Seq/√Len: 2159.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
226_I299_A4.6311.00
255_R282_E3.6951.00
177_L200_A3.1561.00
302_F324_A3.0871.00
123_I403_L2.9481.00
224_E257_R2.8881.00
222_P255_R2.7131.00
385_K389_D2.6881.00
257_R284_P2.6701.00
265_E269_R2.6401.00
242_E271_L2.6381.00
246_Q361_A2.6361.00
250_Q365_A2.6121.00
227_S237_L2.5521.00
248_K280_V2.3671.00
186_A190_Q2.2761.00
242_E357_A2.2611.00
223_L368_S2.2111.00
33_E119_A2.1731.00
236_G239_V2.1471.00
33_E117_F2.1271.00
246_Q358_R2.1221.00
371_D374_E2.0601.00
335_L340_P2.0581.00
244_C256_Y2.0571.00
259_L295_M2.0521.00
271_L275_Y2.0491.00
363_R366_A1.9821.00
224_E255_R1.9761.00
30_F400_A1.9321.00
257_R282_E1.8901.00
343_V349_G1.8831.00
224_E298_D1.8711.00
225_I301_V1.8701.00
348_S385_K1.8691.00
335_L353_P1.8561.00
123_I149_I1.8501.00
328_G383_R1.8501.00
344_E347_K1.8281.00
366_A374_E1.8271.00
375_L379_V1.8201.00
264_W267_R1.8181.00
236_G307_V1.7991.00
394_V398_E1.7951.00
223_L300_D1.7891.00
183_D186_A1.7861.00
353_P359_A1.7651.00
4_G119_A1.7651.00
28_M397_R1.7551.00
261_I265_E1.7501.00
350_W363_R1.7451.00
245_R275_Y1.7301.00
205_G392_Q1.7021.00
302_F329_I1.6961.00
366_A371_D1.6831.00
186_A201_V1.6821.00
273_E278_E1.6751.00
148_T175_G1.6721.00
240_A256_Y1.6581.00
228_V292_V1.6281.00
365_A369_Q1.6281.00
287_K291_E1.6231.00
183_D341_E1.6141.00
347_K381_R1.6001.00
177_L402_L1.6001.00
244_C254_F1.5871.00
180_P199_I1.5601.00
4_G35_L1.5521.00
374_E377_P1.5361.00
356_D359_A1.5321.00
240_A303_L1.5261.00
247_L365_A1.5251.00
24_A27_D1.5171.00
24_A393_Q1.5051.00
359_A363_R1.5021.00
350_W366_A1.4981.00
186_A199_I1.4921.00
344_E385_K1.4901.00
291_E294_A1.4881.00
384_E388_H1.4741.00
344_E348_S1.4741.00
24_A396_N1.4701.00
377_P381_R1.4461.00
19_L23_T1.4451.00
288_P291_E1.4361.00
183_D201_V1.4271.00
28_M400_A1.4241.00
259_L284_P1.4171.00
234_K314_M1.4161.00
287_K295_M1.3771.00
239_V307_V1.3691.00
239_V357_A1.3611.00
238_H271_L1.3561.00
333_S339_I1.3521.00
357_A361_A1.3501.00
225_I364_L1.3431.00
5_F25_F1.3411.00
33_E61_L1.3371.00
224_E300_D1.3341.00
243_A360_L1.3321.00
270_T274_Q1.3211.00
381_R384_E1.3181.00
231_L258_I1.3181.00
219_P222_P1.3171.00
299_A329_I1.3101.00
259_L287_K1.3071.00
352_V363_R1.3021.00
257_R295_M1.3011.00
124_A146_I1.2991.00
179_L399_L1.2791.00
225_I303_L1.2691.00
284_P287_K1.2661.00
271_L274_Q1.2661.00
246_Q357_A1.2621.00
398_E401_S1.2541.00
237_L258_I1.2501.00
183_D187_G1.2381.00
180_P186_A1.2371.00
227_S240_A1.2251.00
394_V397_R1.2201.00
211_F327_V1.2131.00
359_A362_Q1.2131.00
226_I259_L1.2081.00
223_L301_V1.2021.00
5_F125_H1.1981.00
376_A380_K1.1941.00
266_R269_R1.1901.00
257_R299_A1.1851.00
293_K327_V1.1811.00
269_R273_E1.1791.00
33_E59_R1.1791.00
266_R270_T1.1761.00
317_I322_M1.1751.00
306_S315_E1.1641.00
187_G190_Q1.1641.00
179_L402_L1.1591.00
26_I29_G1.1561.00
304_L321_L1.1551.00
245_R249_E1.1481.00
321_L339_I1.1461.00
17_F319_V1.1421.00
345_A363_R1.1331.00
151_H319_V1.1231.00
307_V355_N1.1191.00
292_V296_L1.1151.00
372_T375_L1.1131.00
5_F22_I1.1111.00
223_L254_F1.1081.00
210_R293_K1.1011.00
333_S351_L1.1001.00
272_I283_M1.0991.00
379_V382_A1.0971.00
361_A365_A1.0901.00
190_Q196_R1.0881.00
297_D327_V1.0861.00
328_G380_K1.0821.00
24_A28_M1.0811.00
391_N394_V1.0801.00
9_K12_L1.0771.00
239_V334_T1.0721.00
224_E299_A1.0661.00
261_I286_F1.0661.00
238_H307_V1.0641.00
222_P253_A1.0611.00
367_F378_V1.0601.00
334_T352_V1.0581.00
305_P360_L1.0571.00
230_R234_K1.0561.00
296_L327_V1.0551.00
181_I185_W1.0541.00
304_L320_A1.0501.00
246_Q250_Q1.0481.00
246_Q249_E1.0471.00
180_P189_L1.0391.00
337_S340_P1.0351.00
350_W378_V1.0321.00
238_H242_E1.0291.00
329_I379_V1.0251.00
392_Q395_I1.0191.00
223_L253_A1.0191.00
211_F293_K1.0151.00
395_I398_E1.0141.00
239_V355_N1.0131.00
374_E378_V1.0101.00
302_F320_A1.0101.00
247_L254_F1.0041.00
350_W367_F1.0021.00
22_I34_I0.9991.00
362_Q366_A0.9971.00
187_G191_K0.9941.00
181_I204_M0.9931.00
284_P295_M0.9921.00
257_R298_D0.9891.00
226_I295_M0.9861.00
318_P339_I0.9801.00
228_V259_L0.9801.00
181_I316_G0.9781.00
228_V296_L0.9721.00
108_A112_Q0.9721.00
315_E318_P0.9711.00
195_P198_K0.9691.00
247_L364_L0.9691.00
289_S292_V0.9691.00
239_V242_E0.9691.00
325_M331_V0.9671.00
372_T376_A0.9641.00
3_V30_F0.9621.00
124_A148_T0.9611.00
111_G139_L0.9551.00
352_V360_L0.9501.00
272_I277_L0.9471.00
241_I272_I0.9471.00
146_I175_G0.9461.00
338_G341_E0.9431.00
225_I244_C0.9401.00
180_P185_W0.9351.00
325_M382_A0.9341.00
235_K315_E0.9341.00
293_K297_D0.9311.00
380_K384_E0.9281.00
333_S343_V0.9281.00
238_H267_R0.9271.00
20_N392_Q0.9251.00
242_E275_Y0.9231.00
17_F151_H0.9181.00
395_I399_L0.9151.00
397_R401_S0.9131.00
324_A329_I0.9111.00
267_R270_T0.9081.00
254_F368_S0.9071.00
125_H151_H0.9061.00
353_P356_D0.9041.00
113_V117_F0.9031.00
244_C280_V0.9021.00
208_M391_N0.9011.00
204_M319_V0.8981.00
379_V383_R0.8971.00
232_T263_P0.8961.00
188_R191_K0.8931.00
247_L368_S0.8881.00
332_V352_V0.8881.00
20_N23_T0.8871.00
245_R248_K0.8871.00
207_D210_R0.8861.00
358_R362_Q0.8831.00
205_G395_I0.8821.00
301_V367_F0.8821.00
378_V381_R0.8761.00
321_L342_L0.8741.00
240_A305_P0.8711.00
350_W374_E0.8701.00
267_R271_L0.8691.00
321_L331_V0.8691.00
348_S382_A0.8681.00
376_A379_V0.8681.00
239_V360_L0.8581.00
330_P379_V0.8571.00
226_I296_L0.8571.00
175_G178_M0.8571.00
270_T273_E0.8561.00
207_D211_F0.8541.00
22_I26_I0.8541.00
243_A361_A0.8531.00
202_S402_L0.8521.00
227_S258_I0.8491.00
147_A179_L0.8481.00
16_T20_N0.8411.00
229_A237_L0.8391.00
303_L364_L0.8381.00
385_K390_F0.8341.00
362_Q365_A0.8301.00
227_S305_P0.8261.00
321_L333_S0.8241.00
391_N395_I0.8211.00
304_L339_I0.8201.00
26_I32_V0.8191.00
332_V363_R0.8171.00
247_L361_A0.8171.00
272_I281_V0.8161.00
248_K252_V0.8131.00
301_V364_L0.8121.00
336_H339_I0.8051.00
184_L187_G0.8041.00
227_S302_F0.8031.00
203_R341_E0.8021.00
170_Q174_R0.8021.00
330_P367_F0.8021.00
200_A398_E0.8011.00
200_A402_L0.8001.00
17_F149_I0.7951.00
247_L252_V0.7951.00
296_L329_I0.7941.00
255_R280_V0.7931.00
179_L202_S0.7921.00
152_G234_K0.7911.00
203_R390_F0.7891.00
295_M298_D0.7881.00
235_K320_A0.7861.00
219_P255_R0.7791.00
250_Q362_Q0.7741.00
345_A348_S0.7741.00
325_M348_S0.7671.00
318_P321_L0.7661.00
121_V403_L0.7641.00
242_E246_Q0.7641.00
243_A357_A0.7631.00
17_F204_M0.7621.00
151_H181_I0.7611.00
20_N207_D0.7611.00
233_E238_H0.7591.00
190_Q199_I0.7581.00
7_L18_V0.7561.00
397_R400_A0.7531.00
294_A297_D0.7531.00
343_V351_L0.7531.00
18_V125_H0.7521.00
145_K176_D0.7521.00
21_Q399_L0.7511.00
392_Q396_N0.7501.00
242_E245_R0.7491.00
214_R297_D0.7481.00
28_M393_Q0.7471.00
125_H149_I0.7431.00
104_L108_A0.7431.00
20_N24_A0.7421.00
228_V302_F0.7401.00
235_K306_S0.7401.00
250_Q361_A0.7401.00
181_I319_V0.7371.00
294_A298_D0.7361.00
245_R277_L0.7351.00
325_M385_K0.7341.00
335_L351_L0.7301.00
393_Q397_R0.7271.00
203_R398_E0.7251.00
345_A350_W0.7221.00
5_F123_I0.7221.00
272_I275_Y0.7211.00
332_V367_F0.7211.00
225_I254_F0.7201.00
124_A130_G0.7201.00
124_A133_A0.7171.00
137_R146_I0.7161.00
250_Q369_Q0.7141.00
227_S256_Y0.7101.00
366_A369_Q0.7101.00
289_S293_K0.7081.00
317_I390_F0.7061.00
57_R63_D0.7041.00
123_I399_L0.7041.00
300_D375_L0.7021.00
287_K292_V0.7021.00
377_P380_K0.7011.00
371_D375_L0.7001.00
22_I32_V0.6981.00
322_M390_F0.6981.00
169_Q173_R0.6981.00
58_T61_L0.6971.00
260_G285_G0.6961.00
147_A177_L0.6961.00
304_L318_P0.6931.00
220_A223_L0.6921.00
231_L265_E0.6921.00
378_V382_A0.6861.00
16_T289_S0.6851.00
121_V147_A0.6841.00
184_L188_R0.6841.00
233_E307_V0.6831.00
325_M379_V0.6811.00
223_L372_T0.6791.00
269_R283_M0.6781.00
28_M396_N0.6771.00
399_L403_L0.6741.00
373_D377_P0.6711.00
23_T56_A0.6701.00
208_M326_A0.6691.00
225_I368_S0.6691.00
334_T360_L0.6681.00
57_R62_Q0.6661.00
233_E264_W0.6631.00
367_F375_L0.6631.00
33_E37_L0.6601.00
383_R386_V0.6601.00
147_A403_L0.6591.00
135_K139_L0.6591.00
5_F32_V0.6591.00
107_S111_G0.6581.00
303_L360_L0.6571.00
236_G305_P0.6561.00
122_F144_G0.6531.00
202_S399_L0.6521.00
56_A63_D0.6521.00
373_D376_A0.6521.00
27_D393_Q0.6501.00
268_L283_M0.6491.00
200_A203_R0.6491.00
358_R361_A0.6431.00
296_L320_A0.6391.00
17_F399_L0.6391.00
111_G115_T0.6361.00
340_P351_L0.6351.00
262_G265_E0.6341.00
334_T354_E0.6331.00
223_L226_I0.6311.00
352_V359_A0.6291.00
348_S381_R0.6291.00
166_P169_Q0.6281.00
26_I30_F0.6261.00
346_D366_A0.6241.00
332_V364_L0.6231.00
149_I399_L0.6221.00
343_V348_S0.6201.00
244_C247_L0.6191.00
382_A385_K0.6171.00
108_A111_G0.6171.00
17_F125_H0.6151.00
184_L340_P0.6121.00
229_A235_K0.6081.00
57_R61_L0.6081.00
137_R176_D0.6051.00
23_T27_D0.6041.00
295_M299_A0.6031.00
238_H264_W0.6021.00
236_G355_N0.6021.00
16_T19_L0.6011.00
381_R385_K0.6011.00
253_A280_V0.6001.00
182_S185_W0.5991.00
20_N319_V0.5951.00
203_R317_I0.5951.00
166_P170_Q0.5951.00
142_I145_K0.5941.00
210_R289_S0.5921.00
151_H204_M0.5901.00
241_I281_V0.5901.00
4_G122_F0.5901.00
183_D203_R0.5881.00
100_E103_N0.5881.00
238_H268_L0.5871.00
302_F331_V0.5841.00
29_G400_A0.5811.00
243_A364_L0.5791.00
252_V369_Q0.5791.00
320_A324_A0.5781.00
231_L237_L0.5761.00
59_R62_Q0.5761.00
162_N165_T0.5761.00
224_E295_M0.5761.00
114_A119_A0.5751.00
318_P322_M0.5751.00
202_S398_E0.5731.00
115_T142_I0.5721.00
113_V116_P0.5711.00
186_A196_R0.5691.00
221_T253_A0.5691.00
162_N166_P0.5691.00
230_R235_K0.5681.00
256_Y281_V0.5661.00
185_W188_R0.5631.00
35_L113_V0.5621.00
119_A144_G0.5611.00
389_D394_V0.5611.00
306_S320_A0.5601.00
212_S297_D0.5591.00
226_I329_I0.5541.00
248_K254_F0.5541.00
240_A244_C0.5541.00
4_G33_E0.5531.00
259_L292_V0.5531.00
389_D398_E0.5501.00
226_I257_R0.5491.00
322_M386_V0.5491.00
226_I302_F0.5481.00
17_F21_Q0.5481.00
26_I57_R0.5471.00
301_V332_V0.5471.00
112_Q116_P0.5451.00
223_L375_L0.5451.00
296_L302_F0.5451.00
193_G196_R0.5441.00
149_I179_L0.5411.00
109_I112_Q0.5401.00
209_T212_S0.5391.00
23_T26_I0.5381.00
325_M386_V0.5381.00
31_E59_R0.5351.00
24_A392_Q0.5351.00
5_F403_L0.5341.00
237_L264_W0.5331.00
102_R105_I0.5331.00
305_P334_T0.5331.00
300_D372_T0.5321.00
328_G379_V0.5311.00
212_S215_P0.5301.00
239_V243_A0.5281.00
146_I176_D0.5281.00
380_K383_R0.5271.00
137_R142_I0.5261.00
152_G157_S0.5251.00
272_I278_E0.5251.00
396_N400_A0.5251.00
301_V375_L0.5251.00
130_G133_A0.5241.00
241_I268_L0.5241.00
301_V368_S0.5241.00
170_Q173_R0.5231.00
241_I271_L0.5231.00
102_R106_L0.5221.00
163_H166_P0.5221.00
269_R272_I0.5221.00
180_P201_V0.5211.00
324_A331_V0.5191.00
375_L378_V0.5181.00
315_E320_A0.5181.00
154_D157_S0.5181.00
59_R117_F0.5181.00
138_E174_R0.5161.00
29_G32_V0.5141.00
365_A368_S0.5081.00
259_L299_A0.5081.00
230_R320_A0.5071.00
336_H354_E0.5071.00
292_V295_M0.5071.00
322_M342_L0.5061.00
268_L271_L0.5051.00
261_I285_G0.5041.00
185_W316_G0.5041.00
121_V145_K0.5021.00
243_A303_L0.5001.00
330_P375_L0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2r60A 1 0.9212 100 0.164 Contact Map
3c48A 2 0.8966 100 0.176 Contact Map
4n9wA 1 0.8424 100 0.178 Contact Map
1rzuA 1 0.9483 100 0.199 Contact Map
2x0dA 2 0.835 100 0.207 Contact Map
3froA 3 0.9261 100 0.207 Contact Map
3okpA 1 0.8719 100 0.208 Contact Map
3s28A 4 0.9335 100 0.208 Contact Map
2jjmA 4 0.8571 100 0.208 Contact Map
4rbnA 3 0.936 100 0.215 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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