GREMLIN Database
MBHT - Hydrogenase-2 small chain
UniProt: P69741 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13006
Length: 372 (322)
Sequences: 769 (392)
Seq/√Len: 21.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
268_T294_G4.8601.00
244_A261_L4.5631.00
135_E143_K3.9501.00
270_G296_N3.5991.00
110_K147_G3.3971.00
100_E104_N3.3931.00
160_W218_N3.1271.00
123_L174_V2.9880.99
55_G58_E2.8990.99
113_Y147_G2.8090.99
154_I198_F2.7510.99
115_L144_A2.5820.98
245_K260_H2.5540.98
152_I202_V2.4890.98
197_N271_N2.4570.98
112_Q149_A2.4380.98
56_A137_I2.4180.98
103_H143_K2.4160.97
156_S160_W2.3560.97
57_Q120_S2.3550.97
61_G162_G2.2080.96
58_E164_A2.2020.96
332_V335_G2.1940.96
91_S98_V2.1710.95
135_E139_D2.1520.95
26_A39_M2.1480.95
125_D128_I2.1130.94
160_W298_E2.0760.94
50_P83_S2.0610.94
130_C137_I2.0610.94
113_Y146_E2.0370.93
215_D219_R2.0330.93
283_V287_A2.0190.93
308_Q311_N2.0160.93
198_F202_V1.9550.92
276_Q287_A1.9480.92
204_H212_P1.9110.91
271_N275_L1.9070.91
193_P247_F1.8920.90
63_T87_H1.8820.90
138_V142_R1.8770.90
188_I193_P1.8200.89
115_L145_A1.8010.88
124_K172_G1.8000.88
102_K144_A1.7760.87
121_I141_I1.7590.87
123_L138_V1.7500.86
193_P198_F1.7440.86
306_I313_E1.7430.86
194_N197_N1.7380.86
307_H311_N1.7290.86
43_V203_A1.6990.85
62_C193_P1.6840.84
115_L141_I1.6470.83
51_V84_L1.6430.83
247_F261_L1.6260.82
160_W189_P1.6140.82
55_G118_D1.6120.81
307_H310_A1.6090.81
24_A28_T1.6040.81
152_I198_F1.6030.81
204_H210_K1.5850.80
149_A205_I1.5750.80
237_H242_R1.5680.80
55_G62_C1.5660.80
104_N108_K1.5280.78
116_V198_F1.5040.77
55_G60_T1.5010.77
331_N335_G1.4960.76
217_K298_E1.4840.76
91_S102_K1.4700.75
209_G212_P1.4660.75
50_P85_E1.4500.74
122_P171_T1.4350.73
186_I211_P1.4230.73
124_K128_I1.4220.72
145_A151_I1.4030.71
60_T193_P1.4010.71
331_N334_A1.3990.71
52_I106_L1.3850.70
306_I312_V1.3850.70
204_H209_G1.3770.70
58_E62_C1.3770.70
235_R239_D1.3750.70
332_V336_A1.3650.69
65_S277_F1.3630.69
156_S299_G1.3590.69
180_L183_K1.3200.67
123_L127_G1.3140.66
188_I224_Y1.3130.66
36_A40_A1.3110.66
328_E332_V1.3080.66
17_D20_K1.3020.66
53_W215_D1.2830.64
121_I138_V1.2710.64
306_I310_A1.2710.64
66_L74_V1.2690.63
50_P109_Y1.2500.62
194_N248_G1.2460.62
204_H208_Y1.2400.62
334_A337_I1.2350.61
121_I153_A1.2270.61
107_E110_K1.2010.59
329_G332_V1.2010.59
194_N271_N1.2000.59
192_P265_G1.1990.59
306_I311_N1.1920.59
55_G164_A1.1920.59
21_L25_L1.1880.58
104_N109_Y1.1870.58
335_G338_G1.1820.58
164_A171_T1.1760.57
77_L151_I1.1730.57
308_Q312_V1.1620.57
85_E101_N1.1620.57
63_T118_D1.1610.56
124_K129_Y1.1590.56
135_E140_H1.1560.56
151_I185_V1.1520.56
20_K24_A1.1490.56
85_E109_Y1.1430.55
106_L113_Y1.1420.55
21_L43_V1.1390.55
56_A130_C1.1380.55
294_G297_E1.1340.55
145_A173_A1.1330.55
158_S175_S1.1240.54
223_A271_N1.1200.54
84_L193_P1.1180.54
233_E286_V1.1100.53
124_K127_G1.1030.53
103_H107_E1.0930.52
194_N277_F1.0840.51
179_V182_G1.0820.51
329_G333_S1.0810.51
324_V327_K1.0800.51
79_L130_C1.0710.50
179_V183_K1.0630.50
56_A141_I1.0610.50
131_M136_P1.0610.50
120_S173_A1.0600.50
330_G334_A1.0600.50
233_E271_N1.0570.49
73_T76_N1.0560.49
58_E193_P1.0550.49
57_Q122_P1.0430.48
188_I202_V1.0410.48
102_K203_A1.0380.48
80_E217_K1.0350.48
270_G301_G1.0340.48
107_E147_G1.0210.47
57_Q173_A1.0200.47
61_G154_I1.0170.47
50_P244_A1.0150.47
186_I213_K1.0110.46
117_V137_I1.0100.46
125_D129_Y1.0100.46
283_V288_I1.0090.46
325_N335_G1.0000.46
208_Y212_P0.9990.46
180_L184_T0.9970.45
306_I309_L0.9970.45
264_K297_E0.9950.45
320_Q323_D0.9950.45
154_I202_V0.9910.45
335_G339_L0.9900.45
312_V321_K0.9890.45
187_N298_E0.9850.45
144_A151_I0.9850.45
250_E254_E0.9850.45
52_I102_K0.9840.45
196_H275_L0.9830.44
52_I105_A0.9780.44
233_E287_A0.9770.44
331_N339_L0.9750.44
329_G337_I0.9750.44
110_K146_E0.9680.43
123_L179_V0.9650.43
160_W187_N0.9630.43
56_A121_I0.9610.43
153_A158_S0.9590.43
57_Q137_I0.9580.43
32_S43_V0.9570.43
139_D216_D0.9560.43
38_E41_E0.9540.43
98_V105_A0.9530.42
205_I210_K0.9500.42
146_E183_K0.9470.42
104_N218_N0.9430.42
15_R18_F0.9430.42
321_K326_A0.9350.41
65_S334_A0.9340.41
189_P260_H0.9340.41
271_N277_F0.9320.41
159_A256_W0.9200.40
261_L313_E0.9180.40
186_I214_L0.9120.40
127_G138_V0.9100.40
151_I183_K0.9090.40
227_L269_Y0.9070.39
41_E45_N0.9000.39
188_I198_F0.8960.39
165_A299_G0.8930.39
77_L199_L0.8860.38
62_C285_P0.8860.38
197_N223_A0.8840.38
267_E310_A0.8800.38
69_A199_L0.8740.37
306_I314_N0.8720.37
55_G88_E0.8720.37
197_N277_F0.8710.37
247_F291_P0.8690.37
113_Y149_A0.8590.36
127_G201_T0.8520.36
51_V114_V0.8510.36
128_I131_M0.8480.36
115_L151_I0.8470.36
159_A260_H0.8450.36
146_E249_D0.8400.35
117_V141_I0.8350.35
22_C94_F0.8320.35
101_N105_A0.8250.34
136_P160_W0.8200.34
196_H277_F0.8180.34
193_P248_G0.8180.34
139_D143_K0.8130.34
35_A38_E0.8070.33
65_S153_A0.8070.33
66_L284_W0.8050.33
21_L102_K0.8030.33
329_G335_G0.8010.33
328_E333_S0.8000.33
13_I40_A0.7980.33
156_S159_A0.7980.33
65_S174_V0.7960.33
197_N233_E0.7960.33
133_A139_D0.7920.32
215_D300_I0.7910.32
120_S164_A0.7910.32
26_A31_L0.7870.32
141_I145_A0.7860.32
106_L147_G0.7850.32
197_N275_L0.7840.32
73_T199_L0.7830.32
51_V107_E0.7830.32
245_K249_D0.7830.32
90_L305_G0.7830.32
152_I176_L0.7820.32
113_Y144_A0.7820.32
26_A35_A0.7790.32
57_Q164_A0.7790.32
326_A330_G0.7770.31
139_D142_R0.7760.31
64_E86_Y0.7720.31
128_I136_P0.7710.31
103_H135_E0.7700.31
271_N285_P0.7670.31
186_I243_F0.7660.31
68_R193_P0.7620.31
273_S284_W0.7610.30
332_V339_L0.7580.30
328_E339_L0.7580.30
154_I162_G0.7520.30
109_Y112_Q0.7510.30
326_A333_S0.7500.30
56_A285_P0.7480.30
28_T69_A0.7470.30
332_V337_I0.7450.30
233_E284_W0.7440.30
68_R301_G0.7420.29
38_E156_S0.7390.29
50_P111_G0.7390.29
141_I181_P0.7380.29
271_N284_W0.7350.29
324_V333_S0.7340.29
65_S129_Y0.7310.29
73_T198_F0.7310.29
23_A27_A0.7300.29
97_Q100_E0.7290.29
49_P114_V0.7290.29
118_D133_A0.7270.29
336_A339_L0.7260.29
136_P186_I0.7240.28
334_A339_L0.7220.28
64_E68_R0.7210.28
194_N233_E0.7210.28
114_V147_G0.7180.28
236_P241_G0.7180.28
56_A279_D0.7170.28
82_I188_I0.7170.28
124_K138_V0.7150.28
20_K188_I0.7150.28
123_L172_G0.7150.28
101_N104_N0.7130.28
69_A224_Y0.7120.28
327_K330_G0.7110.28
32_S291_P0.7100.28
105_A109_Y0.7100.28
12_G173_A0.7100.28
188_I197_N0.7090.28
19_M36_A0.7080.28
15_R20_K0.7080.28
132_V140_H0.7070.27
231_H273_S0.7020.27
221_T225_G0.7000.27
204_H207_T0.7000.27
125_D175_S0.6970.27
150_A211_P0.6960.27
66_L114_V0.6950.27
55_G193_P0.6940.27
231_H236_P0.6940.27
322_P331_N0.6940.27
63_T94_F0.6920.27
158_S286_V0.6920.27
223_A237_H0.6900.27
66_L77_L0.6860.26
89_V98_V0.6830.26
102_K136_P0.6810.26
177_Q187_N0.6770.26
264_K294_G0.6770.26
106_L111_G0.6760.26
189_P211_P0.6740.26
60_T218_N0.6730.26
330_G335_G0.6680.25
58_E285_P0.6670.25
234_R289_G0.6670.25
242_R252_H0.6670.25
208_Y235_R0.6670.25
51_V82_I0.6630.25
118_D175_S0.6620.25
334_A338_G0.6610.25
41_E175_S0.6590.25
153_A159_A0.6590.25
55_G63_T0.6540.25
33_S36_A0.6530.25
235_R241_G0.6520.25
66_L146_E0.6500.25
302_F306_I0.6490.24
325_N330_G0.6470.24
38_E45_N0.6470.24
31_L179_V0.6460.24
99_E255_G0.6460.24
32_S217_K0.6440.24
69_A281_G0.6440.24
223_A248_G0.6420.24
62_C156_S0.6390.24
63_T70_T0.6390.24
305_G308_Q0.6370.24
126_N129_Y0.6350.24
142_R181_P0.6330.24
311_N314_N0.6330.24
327_K331_N0.6330.24
30_G62_C0.6320.24
116_V120_S0.6320.24
192_P228_I0.6310.24
238_F327_K0.6300.24
68_R285_P0.6280.23
63_T200_A0.6220.23
153_A338_G0.6210.23
319_S324_V0.6210.23
199_L277_F0.6190.23
331_N338_G0.6170.23
194_N285_P0.6170.23
282_G296_N0.6170.23
228_I270_G0.6160.23
67_L121_I0.6130.23
71_H224_Y0.6120.23
277_F284_W0.6100.23
41_E339_L0.6090.23
16_R19_M0.6090.23
266_P294_G0.6080.22
194_N284_W0.6080.22
228_I235_R0.6080.22
145_A239_D0.6070.22
63_T152_I0.6070.22
328_E335_G0.6050.22
231_H238_F0.6050.22
93_A101_N0.6030.22
230_E235_R0.6030.22
213_K221_T0.6000.22
156_S189_P0.6000.22
223_A277_F0.6000.22
333_S337_I0.5990.22
106_L144_A0.5990.22
254_E275_L0.5980.22
190_G255_G0.5970.22
185_V218_N0.5960.22
234_R258_L0.5940.22
28_T63_T0.5930.22
62_C65_S0.5910.22
63_T278_C0.5900.22
124_K174_V0.5890.22
333_S339_L0.5890.22
180_L185_V0.5890.22
57_Q171_T0.5880.22
268_T272_C0.5870.21
158_S176_L0.5870.21
110_K172_G0.5870.21
330_G337_I0.5860.21
74_V79_L0.5860.21
153_A187_N0.5850.21
328_E337_I0.5840.21
300_I306_I0.5830.21
296_N301_G0.5830.21
127_G176_L0.5810.21
197_N284_W0.5800.21
86_Y99_E0.5790.21
87_H162_G0.5780.21
88_E244_A0.5780.21
199_L242_R0.5760.21
55_G194_N0.5740.21
173_A238_F0.5730.21
28_T299_G0.5730.21
45_N49_P0.5720.21
259_Y285_P0.5720.21
325_N331_N0.5710.21
141_I176_L0.5690.21
330_G339_L0.5630.20
325_N333_S0.5620.20
93_A286_V0.5610.20
22_C26_A0.5600.20
142_R210_K0.5580.20
68_R270_G0.5580.20
297_E336_A0.5570.20
233_E277_F0.5560.20
117_V176_L0.5540.20
156_S301_G0.5540.20
58_E88_E0.5540.20
194_N198_F0.5510.20
114_V209_G0.5510.20
249_D291_P0.5510.20
167_G221_T0.5470.20
329_G339_L0.5440.20
13_I36_A0.5420.19
46_P198_F0.5410.19
248_G252_H0.5400.19
321_K329_G0.5390.19
306_I322_P0.5390.19
330_G333_S0.5380.19
227_L283_V0.5370.19
97_Q239_D0.5370.19
66_L81_T0.5350.19
187_N218_N0.5340.19
36_A186_I0.5340.19
106_L114_V0.5330.19
13_I50_P0.5320.19
255_G289_G0.5310.19
53_W228_I0.5300.19
17_D34_K0.5290.19
100_E146_E0.5290.19
80_E87_H0.5270.19
30_G46_P0.5250.19
121_I151_I0.5250.19
243_F258_L0.5250.19
144_A147_G0.5250.19
168_V285_P0.5230.19
117_V156_S0.5230.19
100_E307_H0.5210.19
121_I176_L0.5200.19
164_A228_I0.5190.19
92_A150_A0.5170.18
237_H256_W0.5160.18
258_L289_G0.5160.18
32_S188_I0.5150.18
326_A332_V0.5140.18
16_R20_K0.5130.18
150_A183_K0.5130.18
15_R19_M0.5120.18
27_A30_G0.5120.18
288_I300_I0.5100.18
63_T89_V0.5100.18
293_Y306_I0.5090.18
278_C297_E0.5070.18
202_V337_I0.5060.18
236_P242_R0.5060.18
205_I209_G0.5050.18
219_R300_I0.5040.18
26_A101_N0.5040.18
54_I141_I0.5020.18
13_I21_L0.5020.18
224_Y268_T0.5010.18
328_E331_N0.5000.18
57_Q130_C0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1h2aS 1 0.7124 100 0.276 Contact Map
4ue3S 2 0.7124 100 0.295 Contact Map
2wpnA 1 0.6882 100 0.305 Contact Map
4iucS 1 0.7124 100 0.312 Contact Map
3ayxB 2 0.7258 100 0.341 Contact Map
3myrA 3 0.7177 100 0.359 Contact Map
4u9hS 1 0.707 100 0.362 Contact Map
4kn9S 1 0.6935 100 0.367 Contact Map
4uqlA 1 0.7043 100 0.371 Contact Map
1yq9A 2 0.6962 100 0.372 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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