GREMLIN Database
FIEF - Ferrous-iron efflux pump FieF
UniProt: P69380 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11873
Length: 300 (277)
Sequences: 10520 (7286)
Seq/√Len: 437.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
177_R181_L3.5741.00
192_Y196_R3.2371.00
203_Q208_R3.0601.00
247_I282_I2.9951.00
222_V247_I2.8071.00
232_H248_H2.7671.00
219_I223_T2.5981.00
30_A120_V2.5201.00
221_I275_F2.4791.00
221_I274_R2.4591.00
99_L103_Q2.4151.00
28_I43_L2.3581.00
98_F184_L2.2951.00
68_D75_H2.2591.00
87_S198_G2.1391.00
222_V267_V2.1361.00
170_L178_A2.1271.00
139_V148_R2.1031.00
225_W270_A2.0961.00
199_Y203_Q2.0841.00
229_S252_E2.0711.00
78_A206_L2.0491.00
119_I123_V2.0291.00
31_W40_L2.0191.00
269_Q273_R1.9201.00
98_F187_G1.9181.00
10_S64_L1.9031.00
225_W267_V1.9031.00
225_W266_Q1.8991.00
221_I270_A1.8721.00
216_Q220_D1.8571.00
71_H75_H1.8311.00
118_V165_L1.8211.00
253_D258_V1.8101.00
261_H283_H1.7631.00
217_E274_R1.7621.00
249_L267_V1.7571.00
77_K207_D1.7451.00
215_R236_T1.7251.00
29_F33_Y1.7141.00
10_S61_R1.6951.00
103_Q107_S1.6881.00
14_I18_A1.6791.00
195_L199_Y1.6621.00
262_M266_Q1.6611.00
259_Q262_M1.6441.00
211_P214_E1.6401.00
95_L99_L1.6211.00
97_L183_A1.6091.00
184_L188_I1.6031.00
260_A284_Q1.5881.00
257_L260_A1.5771.00
136_R140_R1.5661.00
28_I32_W1.5641.00
230_G250_E1.5551.00
167_A182_F1.5421.00
188_I192_Y1.5271.00
270_A274_R1.5161.00
102_I106_I1.5131.00
129_I133_S1.4851.00
118_V169_G1.4801.00
220_D224_S1.4781.00
54_L58_L1.4771.00
266_Q270_A1.4691.00
14_I57_L1.4681.00
148_R200_E1.4511.00
169_G172_W1.4361.00
21_S50_I1.4321.00
163_A182_F1.4181.00
19_M127_C1.4081.00
196_R199_Y1.4071.00
137_W141_R1.4061.00
232_H285_D1.4001.00
45_D153_H1.3941.00
101_G183_A1.3941.00
136_R151_M1.3661.00
73_F246_Q1.3531.00
236_T245_I1.3321.00
34_T117_G1.3261.00
122_I126_I1.3221.00
251_M257_L1.3101.00
177_R180_A1.3041.00
118_V168_L1.2991.00
264_A268_E1.2891.00
170_L173_Y1.2721.00
122_I165_L1.2711.00
251_M263_V1.2711.00
223_T230_G1.2681.00
255_L259_Q1.2671.00
125_L165_L1.2481.00
96_F99_L1.2471.00
216_Q219_I1.2431.00
210_L236_T1.2371.00
217_E221_I1.2351.00
15_A134_F1.2351.00
94_A191_L1.2271.00
214_E217_E1.2131.00
126_I130_I1.2121.00
217_E275_F1.2111.00
59_V82_A1.2051.00
27_K46_S1.2021.00
207_D244_F1.2021.00
130_I133_S1.2011.00
31_W43_L1.1941.00
248_H285_D1.1881.00
62_Y78_A1.1781.00
105_L180_A1.1781.00
68_D71_H1.1751.00
133_S137_W1.1731.00
234_L247_I1.1651.00
18_A22_L1.1651.00
117_G168_L1.1521.00
264_A283_H1.1501.00
264_A284_Q1.1391.00
24_L47_L1.1331.00
225_W263_V1.1211.00
218_I271_I1.1211.00
19_M131_L1.1201.00
261_H286_P1.1191.00
91_S194_A1.1161.00
258_V262_M1.1111.00
257_L284_Q1.1101.00
37_V101_G1.1101.00
212_D216_Q1.1071.00
235_R279_D1.1001.00
70_N75_H1.0991.00
130_I134_F1.0991.00
268_E272_L1.0951.00
105_L177_R1.0921.00
217_E220_D1.0861.00
98_F102_I1.0851.00
71_H203_Q1.0751.00
23_L27_K1.0741.00
150_D153_H1.0711.00
87_S197_M1.0681.00
23_L127_C1.0651.00
100_T103_Q1.0641.00
91_S95_L1.0491.00
55_T82_A1.0471.00
61_R64_L1.0451.00
24_L43_L1.0421.00
232_H250_E1.0401.00
115_G119_I1.0361.00
11_R15_A1.0331.00
224_S270_A1.0321.00
221_I224_S1.0321.00
17_T50_I1.0271.00
80_S204_S1.0221.00
175_W178_A1.0161.00
260_A264_A1.0061.00
24_L28_I1.0051.00
221_I225_W0.9971.00
91_S191_L0.9881.00
218_I236_T0.9871.00
28_I40_L0.9871.00
121_T165_L0.9861.00
227_G259_Q0.9851.00
138_V141_R0.9851.00
261_H264_A0.9841.00
268_E283_H0.9821.00
70_N208_R0.9801.00
62_Y65_Q0.9781.00
91_S195_L0.9751.00
151_M155_Q0.9731.00
245_I280_V0.9711.00
119_I122_I0.9691.00
56_N146_A0.9661.00
133_S136_R0.9611.00
16_A131_L0.9541.00
207_D239_S0.9501.00
254_S258_V0.9501.00
37_V104_H0.9491.00
42_A161_N0.9441.00
101_G184_L0.9421.00
162_G166_L0.9381.00
42_A45_D0.9381.00
17_T57_L0.9381.00
251_M260_A0.9371.00
32_W40_L0.9351.00
265_D269_Q0.9251.00
56_N149_A0.9211.00
25_L29_F0.9201.00
37_V183_A0.9181.00
252_E255_L0.9171.00
105_L184_L0.9161.00
132_V151_M0.9121.00
17_T54_L0.9111.00
227_G263_V0.9071.00
79_E144_S0.8981.00
215_R219_I0.8951.00
250_E287_C0.8841.00
233_D248_H0.8831.00
132_V136_R0.8831.00
210_L243_R0.8821.00
72_S209_A0.8811.00
277_G280_V0.8801.00
100_T104_H0.8801.00
251_M284_Q0.8751.00
88_M92_G0.8691.00
233_D279_D0.8671.00
228_V249_L0.8621.00
139_V151_M0.8551.00
26_I123_V0.8551.00
58_L62_Y0.8541.00
81_L206_L0.8511.00
129_I155_Q0.8491.00
204_S238_Q0.8371.00
153_H157_D0.8321.00
102_I184_L0.8311.00
103_Q106_I0.8251.00
223_T231_A0.8231.00
239_S244_F0.8211.00
261_H285_D0.8211.00
137_W140_R0.8181.00
264_A282_I0.8171.00
229_S287_C0.8121.00
84_L198_G0.8101.00
263_V267_V0.8101.00
209_A215_R0.8061.00
21_S25_L0.8041.00
207_D237_R0.8041.00
167_A178_A0.8021.00
45_D49_D0.8001.00
37_V100_T0.7991.00
73_F237_R0.7951.00
96_F100_T0.7931.00
219_I231_A0.7921.00
12_A134_F0.7901.00
131_L154_Y0.7891.00
20_A46_S0.7861.00
181_L185_G0.7861.00
227_G266_Q0.7801.00
132_V155_Q0.7631.00
92_G96_F0.7621.00
220_D223_T0.7611.00
255_L260_A0.7611.00
152_L155_Q0.7551.00
265_D268_E0.7551.00
58_L61_R0.7501.00
231_A234_L0.7481.00
238_Q243_R0.7461.00
24_L50_I0.7461.00
103_Q109_T0.7441.00
231_A247_I0.7421.00
92_G99_L0.7421.00
268_E280_V0.7381.00
218_I245_I0.7381.00
36_S179_D0.7371.00
28_I47_L0.7351.00
210_L214_E0.7351.00
118_V171_S0.7341.00
271_I280_V0.7341.00
138_V142_T0.7341.00
48_V93_S0.7311.00
182_F186_I0.7281.00
214_E243_R0.7271.00
270_A273_R0.7271.00
26_I120_V0.7261.00
131_L135_Q0.7231.00
60_V146_A0.7231.00
84_L88_M0.7221.00
77_K237_R0.7201.00
10_S14_I0.7141.00
148_R152_L0.7121.00
118_V122_I0.7091.00
111_M114_P0.7081.00
156_S193_S0.7071.00
81_L85_A0.7071.00
178_A182_F0.7061.00
12_A137_W0.7051.00
245_I278_S0.7041.00
227_G252_E0.7041.00
125_L162_G0.7031.00
108_P111_M0.7011.00
27_K43_L0.7001.00
24_L46_S0.7001.00
114_P171_S0.6991.00
235_R246_Q0.6991.00
112_T115_G0.6971.00
271_I282_I0.6921.00
218_I275_F0.6921.00
253_D287_C0.6901.00
84_L201_A0.6891.00
260_A286_P0.6851.00
145_Q204_S0.6831.00
16_A154_Y0.6821.00
116_V119_I0.6821.00
128_T131_L0.6801.00
255_L263_V0.6801.00
18_A21_S0.6801.00
109_T112_T0.6781.00
222_V231_A0.6771.00
10_S57_L0.6771.00
95_L191_L0.6701.00
145_Q200_E0.6701.00
134_F138_V0.6641.00
16_A135_Q0.6641.00
32_W43_L0.6601.00
259_Q266_Q0.6601.00
38_S182_F0.6581.00
266_Q269_Q0.6561.00
83_A146_A0.6561.00
149_A197_M0.6551.00
267_V282_I0.6541.00
169_G173_Y0.6541.00
14_I17_T0.6541.00
70_N203_Q0.6530.99
81_L202_V0.6500.99
57_L61_R0.6500.99
47_L51_G0.6480.99
83_A197_M0.6470.99
63_S79_E0.6440.99
52_A56_N0.6400.99
101_G187_G0.6380.99
124_A128_T0.6360.99
209_A236_T0.6330.99
52_A89_F0.6330.99
185_G189_Y0.6300.99
140_R148_R0.6280.99
189_Y193_S0.6270.99
20_A49_D0.6250.99
283_H286_P0.6250.99
72_S235_R0.6250.99
135_Q150_D0.6240.99
238_Q241_P0.6230.99
246_Q279_D0.6220.99
129_I158_V0.6220.99
94_A194_A0.6210.99
192_Y195_L0.6190.99
45_D90_I0.6170.99
27_K42_A0.6160.99
222_V225_W0.6160.99
135_Q154_Y0.6140.99
260_A263_V0.6140.99
31_W34_T0.6130.99
127_C131_L0.6110.99
12_A138_V0.6080.99
203_Q211_P0.6060.99
29_F32_W0.6040.99
131_L134_F0.6030.99
107_S110_P0.6030.99
210_L215_R0.6010.99
222_V249_L0.5990.99
213_E236_T0.5920.99
16_A53_S0.5920.99
228_V231_A0.5910.99
128_T135_Q0.5900.99
155_Q159_M0.5890.99
267_V270_A0.5880.99
136_R155_Q0.5830.99
16_A150_D0.5820.99
132_V154_Y0.5820.99
87_S201_A0.5780.99
117_G167_A0.5770.99
53_S150_D0.5760.99
51_G54_L0.5710.99
148_R151_M0.5710.99
125_L129_I0.5680.99
113_D116_V0.5680.99
83_A201_A0.5670.99
61_R65_Q0.5670.99
159_M162_G0.5600.98
65_Q75_H0.5560.98
52_A153_H0.5560.98
272_L280_V0.5550.98
164_I167_A0.5540.98
121_T124_A0.5540.98
264_A267_V0.5520.98
93_S96_F0.5510.98
37_V40_L0.5500.98
160_M167_A0.5460.98
13_A17_T0.5440.98
261_H265_D0.5420.98
28_I44_V0.5410.98
173_Y178_A0.5400.98
113_D171_S0.5390.98
20_A50_I0.5390.98
110_P176_H0.5360.98
161_N164_I0.5350.98
128_T154_Y0.5340.98
153_H156_S0.5340.98
69_D215_R0.5340.98
182_F185_G0.5330.98
92_G95_L0.5320.98
249_L282_I0.5320.98
249_L284_Q0.5320.98
101_G180_A0.5280.98
13_A56_N0.5280.98
67_A75_H0.5260.98
171_S174_G0.5260.98
132_V158_V0.5260.98
262_M265_D0.5260.98
166_L170_L0.5230.98
143_Q148_R0.5220.98
84_L205_L0.5170.97
202_V206_L0.5140.97
263_V266_Q0.5140.97
39_I121_T0.5140.97
226_P266_Q0.5130.97
15_A19_M0.5130.97
15_A18_A0.5130.97
225_W228_V0.5120.97
220_D274_R0.5120.97
209_A238_Q0.5120.97
22_L26_I0.5120.97
78_A82_A0.5110.97
73_F235_R0.5090.97
221_I267_V0.5080.97
173_Y176_H0.5060.97
187_G194_A0.5050.97
227_G267_V0.5040.97
227_G255_L0.5040.97
272_L278_S0.5040.97
191_L195_L0.5030.97
234_L245_I0.5020.97
128_T153_H0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j1zP 8 0.98 100 0.093 Contact Map
3h90A 3 0.9433 100 0.125 Contact Map
3bypA 2 0.2667 99.5 0.757 Contact Map
2zztA 2 0.2667 99.4 0.767 Contact Map
3w61A 2 0.2633 99.3 0.785 Contact Map
1ji6A 1 0.42 46.6 0.944 Contact Map
1dlcA 1 0.43 41 0.945 Contact Map
3eb7A 2 0.4233 29.6 0.949 Contact Map
2c9kA 1 0.4233 28.4 0.95 Contact Map
1ib8A 1 0.3233 25 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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