FIEF - ECOLI - GREMLIN Contacts

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FIEF - Ferrous-iron efflux pump FieF

UniProt:	P69380	Sequences: MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALVDSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGIQHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMMNGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEIIDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFPGSDVIIHQDPCSVVPREGKRSMLS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11873
Length:	300 (277)
Sequences:	10520 (7286)
Seq/√Len:	437.7

ECOLI - FIEF ZITB
BACSU - YDFM YDBO YEAB CZCD
HALSA - Q9HP06
SULSO - Q97WI8 Q97WH2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
177_R	181_L	3.574	1.00
192_Y	196_R	3.237	1.00
203_Q	208_R	3.060	1.00
247_I	282_I	2.995	1.00
222_V	247_I	2.807	1.00
232_H	248_H	2.767	1.00
219_I	223_T	2.598	1.00
30_A	120_V	2.520	1.00
221_I	275_F	2.479	1.00
221_I	274_R	2.459	1.00
99_L	103_Q	2.415	1.00
28_I	43_L	2.358	1.00
98_F	184_L	2.295	1.00
68_D	75_H	2.259	1.00
87_S	198_G	2.139	1.00
222_V	267_V	2.136	1.00
170_L	178_A	2.127	1.00
139_V	148_R	2.103	1.00
225_W	270_A	2.096	1.00
199_Y	203_Q	2.084	1.00
229_S	252_E	2.071	1.00
78_A	206_L	2.049	1.00
119_I	123_V	2.029	1.00
31_W	40_L	2.019	1.00
269_Q	273_R	1.920	1.00
98_F	187_G	1.918	1.00
10_S	64_L	1.903	1.00
225_W	267_V	1.903	1.00
225_W	266_Q	1.899	1.00
221_I	270_A	1.872	1.00
216_Q	220_D	1.857	1.00
71_H	75_H	1.831	1.00
118_V	165_L	1.821	1.00
253_D	258_V	1.810	1.00
261_H	283_H	1.763	1.00
217_E	274_R	1.762	1.00
249_L	267_V	1.757	1.00
77_K	207_D	1.745	1.00
215_R	236_T	1.725	1.00
29_F	33_Y	1.714	1.00
10_S	61_R	1.695	1.00
103_Q	107_S	1.688	1.00
14_I	18_A	1.679	1.00
195_L	199_Y	1.662	1.00
262_M	266_Q	1.661	1.00
259_Q	262_M	1.644	1.00
211_P	214_E	1.640	1.00
95_L	99_L	1.621	1.00
97_L	183_A	1.609	1.00
184_L	188_I	1.603	1.00
260_A	284_Q	1.588	1.00
257_L	260_A	1.577	1.00
136_R	140_R	1.566	1.00
28_I	32_W	1.564	1.00
230_G	250_E	1.555	1.00
167_A	182_F	1.542	1.00
188_I	192_Y	1.527	1.00
270_A	274_R	1.516	1.00
102_I	106_I	1.513	1.00
129_I	133_S	1.485	1.00
118_V	169_G	1.480	1.00
220_D	224_S	1.478	1.00
54_L	58_L	1.477	1.00
266_Q	270_A	1.469	1.00
14_I	57_L	1.468	1.00
148_R	200_E	1.451	1.00
169_G	172_W	1.436	1.00
21_S	50_I	1.432	1.00
163_A	182_F	1.418	1.00
19_M	127_C	1.408	1.00
196_R	199_Y	1.407	1.00
137_W	141_R	1.406	1.00
232_H	285_D	1.400	1.00
45_D	153_H	1.394	1.00
101_G	183_A	1.394	1.00
136_R	151_M	1.366	1.00
73_F	246_Q	1.353	1.00
236_T	245_I	1.332	1.00
34_T	117_G	1.326	1.00
122_I	126_I	1.322	1.00
251_M	257_L	1.310	1.00
177_R	180_A	1.304	1.00
118_V	168_L	1.299	1.00
264_A	268_E	1.289	1.00
170_L	173_Y	1.272	1.00
122_I	165_L	1.271	1.00
251_M	263_V	1.271	1.00
223_T	230_G	1.268	1.00
255_L	259_Q	1.267	1.00
125_L	165_L	1.248	1.00
96_F	99_L	1.247	1.00
216_Q	219_I	1.243	1.00
210_L	236_T	1.237	1.00
217_E	221_I	1.235	1.00
15_A	134_F	1.235	1.00
94_A	191_L	1.227	1.00
214_E	217_E	1.213	1.00
126_I	130_I	1.212	1.00
217_E	275_F	1.211	1.00
59_V	82_A	1.205	1.00
27_K	46_S	1.202	1.00
207_D	244_F	1.202	1.00
130_I	133_S	1.201	1.00
31_W	43_L	1.194	1.00
248_H	285_D	1.188	1.00
62_Y	78_A	1.178	1.00
105_L	180_A	1.178	1.00
68_D	71_H	1.175	1.00
133_S	137_W	1.173	1.00
234_L	247_I	1.165	1.00
18_A	22_L	1.165	1.00
117_G	168_L	1.152	1.00
264_A	283_H	1.150	1.00
264_A	284_Q	1.139	1.00
24_L	47_L	1.133	1.00
225_W	263_V	1.121	1.00
218_I	271_I	1.121	1.00
19_M	131_L	1.120	1.00
261_H	286_P	1.119	1.00
91_S	194_A	1.116	1.00
258_V	262_M	1.111	1.00
257_L	284_Q	1.110	1.00
37_V	101_G	1.110	1.00
212_D	216_Q	1.107	1.00
235_R	279_D	1.100	1.00
70_N	75_H	1.099	1.00
130_I	134_F	1.099	1.00
268_E	272_L	1.095	1.00
105_L	177_R	1.092	1.00
217_E	220_D	1.086	1.00
98_F	102_I	1.085	1.00
71_H	203_Q	1.075	1.00
23_L	27_K	1.074	1.00
150_D	153_H	1.071	1.00
87_S	197_M	1.068	1.00
23_L	127_C	1.065	1.00
100_T	103_Q	1.064	1.00
91_S	95_L	1.049	1.00
55_T	82_A	1.047	1.00
61_R	64_L	1.045	1.00
24_L	43_L	1.042	1.00
232_H	250_E	1.040	1.00
115_G	119_I	1.036	1.00
11_R	15_A	1.033	1.00
224_S	270_A	1.032	1.00
221_I	224_S	1.032	1.00
17_T	50_I	1.027	1.00
80_S	204_S	1.022	1.00
175_W	178_A	1.016	1.00
260_A	264_A	1.006	1.00
24_L	28_I	1.005	1.00
221_I	225_W	0.997	1.00
91_S	191_L	0.988	1.00
218_I	236_T	0.987	1.00
28_I	40_L	0.987	1.00
121_T	165_L	0.986	1.00
227_G	259_Q	0.985	1.00
138_V	141_R	0.985	1.00
261_H	264_A	0.984	1.00
268_E	283_H	0.982	1.00
70_N	208_R	0.980	1.00
62_Y	65_Q	0.978	1.00
91_S	195_L	0.975	1.00
151_M	155_Q	0.973	1.00
245_I	280_V	0.971	1.00
119_I	122_I	0.969	1.00
56_N	146_A	0.966	1.00
133_S	136_R	0.961	1.00
16_A	131_L	0.954	1.00
207_D	239_S	0.950	1.00
254_S	258_V	0.950	1.00
37_V	104_H	0.949	1.00
42_A	161_N	0.944	1.00
101_G	184_L	0.942	1.00
162_G	166_L	0.938	1.00
42_A	45_D	0.938	1.00
17_T	57_L	0.938	1.00
251_M	260_A	0.937	1.00
32_W	40_L	0.935	1.00
265_D	269_Q	0.925	1.00
56_N	149_A	0.921	1.00
25_L	29_F	0.920	1.00
37_V	183_A	0.918	1.00
252_E	255_L	0.917	1.00
105_L	184_L	0.916	1.00
132_V	151_M	0.912	1.00
17_T	54_L	0.911	1.00
227_G	263_V	0.907	1.00
79_E	144_S	0.898	1.00
215_R	219_I	0.895	1.00
250_E	287_C	0.884	1.00
233_D	248_H	0.883	1.00
132_V	136_R	0.883	1.00
210_L	243_R	0.882	1.00
72_S	209_A	0.881	1.00
277_G	280_V	0.880	1.00
100_T	104_H	0.880	1.00
251_M	284_Q	0.875	1.00
88_M	92_G	0.869	1.00
233_D	279_D	0.867	1.00
228_V	249_L	0.862	1.00
139_V	151_M	0.855	1.00
26_I	123_V	0.855	1.00
58_L	62_Y	0.854	1.00
81_L	206_L	0.851	1.00
129_I	155_Q	0.849	1.00
204_S	238_Q	0.837	1.00
153_H	157_D	0.832	1.00
102_I	184_L	0.831	1.00
103_Q	106_I	0.825	1.00
223_T	231_A	0.823	1.00
239_S	244_F	0.821	1.00
261_H	285_D	0.821	1.00
137_W	140_R	0.818	1.00
264_A	282_I	0.817	1.00
229_S	287_C	0.812	1.00
84_L	198_G	0.810	1.00
263_V	267_V	0.810	1.00
209_A	215_R	0.806	1.00
21_S	25_L	0.804	1.00
207_D	237_R	0.804	1.00
167_A	178_A	0.802	1.00
45_D	49_D	0.800	1.00
37_V	100_T	0.799	1.00
73_F	237_R	0.795	1.00
96_F	100_T	0.793	1.00
219_I	231_A	0.792	1.00
12_A	134_F	0.790	1.00
131_L	154_Y	0.789	1.00
20_A	46_S	0.786	1.00
181_L	185_G	0.786	1.00
227_G	266_Q	0.780	1.00
132_V	155_Q	0.763	1.00
92_G	96_F	0.762	1.00
220_D	223_T	0.761	1.00
255_L	260_A	0.761	1.00
152_L	155_Q	0.755	1.00
265_D	268_E	0.755	1.00
58_L	61_R	0.750	1.00
231_A	234_L	0.748	1.00
238_Q	243_R	0.746	1.00
24_L	50_I	0.746	1.00
103_Q	109_T	0.744	1.00
231_A	247_I	0.742	1.00
92_G	99_L	0.742	1.00
268_E	280_V	0.738	1.00
218_I	245_I	0.738	1.00
36_S	179_D	0.737	1.00
28_I	47_L	0.735	1.00
210_L	214_E	0.735	1.00
118_V	171_S	0.734	1.00
271_I	280_V	0.734	1.00
138_V	142_T	0.734	1.00
48_V	93_S	0.731	1.00
182_F	186_I	0.728	1.00
214_E	243_R	0.727	1.00
270_A	273_R	0.727	1.00
26_I	120_V	0.726	1.00
131_L	135_Q	0.723	1.00
60_V	146_A	0.723	1.00
84_L	88_M	0.722	1.00
77_K	237_R	0.720	1.00
10_S	14_I	0.714	1.00
148_R	152_L	0.712	1.00
118_V	122_I	0.709	1.00
111_M	114_P	0.708	1.00
156_S	193_S	0.707	1.00
81_L	85_A	0.707	1.00
178_A	182_F	0.706	1.00
12_A	137_W	0.705	1.00
245_I	278_S	0.704	1.00
227_G	252_E	0.704	1.00
125_L	162_G	0.703	1.00
108_P	111_M	0.701	1.00
27_K	43_L	0.700	1.00
24_L	46_S	0.700	1.00
114_P	171_S	0.699	1.00
235_R	246_Q	0.699	1.00
112_T	115_G	0.697	1.00
271_I	282_I	0.692	1.00
218_I	275_F	0.692	1.00
253_D	287_C	0.690	1.00
84_L	201_A	0.689	1.00
260_A	286_P	0.685	1.00
145_Q	204_S	0.683	1.00
16_A	154_Y	0.682	1.00
116_V	119_I	0.682	1.00
128_T	131_L	0.680	1.00
255_L	263_V	0.680	1.00
18_A	21_S	0.680	1.00
109_T	112_T	0.678	1.00
222_V	231_A	0.677	1.00
10_S	57_L	0.677	1.00
95_L	191_L	0.670	1.00
145_Q	200_E	0.670	1.00
134_F	138_V	0.664	1.00
16_A	135_Q	0.664	1.00
32_W	43_L	0.660	1.00
259_Q	266_Q	0.660	1.00
38_S	182_F	0.658	1.00
266_Q	269_Q	0.656	1.00
83_A	146_A	0.656	1.00
149_A	197_M	0.655	1.00
267_V	282_I	0.654	1.00
169_G	173_Y	0.654	1.00
14_I	17_T	0.654	1.00
70_N	203_Q	0.653	0.99
81_L	202_V	0.650	0.99
57_L	61_R	0.650	0.99
47_L	51_G	0.648	0.99
83_A	197_M	0.647	0.99
63_S	79_E	0.644	0.99
52_A	56_N	0.640	0.99
101_G	187_G	0.638	0.99
124_A	128_T	0.636	0.99
209_A	236_T	0.633	0.99
52_A	89_F	0.633	0.99
185_G	189_Y	0.630	0.99
140_R	148_R	0.628	0.99
189_Y	193_S	0.627	0.99
20_A	49_D	0.625	0.99
283_H	286_P	0.625	0.99
72_S	235_R	0.625	0.99
135_Q	150_D	0.624	0.99
238_Q	241_P	0.623	0.99
246_Q	279_D	0.622	0.99
129_I	158_V	0.622	0.99
94_A	194_A	0.621	0.99
192_Y	195_L	0.619	0.99
45_D	90_I	0.617	0.99
27_K	42_A	0.616	0.99
222_V	225_W	0.616	0.99
135_Q	154_Y	0.614	0.99
260_A	263_V	0.614	0.99
31_W	34_T	0.613	0.99
127_C	131_L	0.611	0.99
12_A	138_V	0.608	0.99
203_Q	211_P	0.606	0.99
29_F	32_W	0.604	0.99
131_L	134_F	0.603	0.99
107_S	110_P	0.603	0.99
210_L	215_R	0.601	0.99
222_V	249_L	0.599	0.99
213_E	236_T	0.592	0.99
16_A	53_S	0.592	0.99
228_V	231_A	0.591	0.99
128_T	135_Q	0.590	0.99
155_Q	159_M	0.589	0.99
267_V	270_A	0.588	0.99
136_R	155_Q	0.583	0.99
16_A	150_D	0.582	0.99
132_V	154_Y	0.582	0.99
87_S	201_A	0.578	0.99
117_G	167_A	0.577	0.99
53_S	150_D	0.576	0.99
51_G	54_L	0.571	0.99
148_R	151_M	0.571	0.99
125_L	129_I	0.568	0.99
113_D	116_V	0.568	0.99
83_A	201_A	0.567	0.99
61_R	65_Q	0.567	0.99
159_M	162_G	0.560	0.98
65_Q	75_H	0.556	0.98
52_A	153_H	0.556	0.98
272_L	280_V	0.555	0.98
164_I	167_A	0.554	0.98
121_T	124_A	0.554	0.98
264_A	267_V	0.552	0.98
93_S	96_F	0.551	0.98
37_V	40_L	0.550	0.98
160_M	167_A	0.546	0.98
13_A	17_T	0.544	0.98
261_H	265_D	0.542	0.98
28_I	44_V	0.541	0.98
173_Y	178_A	0.540	0.98
113_D	171_S	0.539	0.98
20_A	50_I	0.539	0.98
110_P	176_H	0.536	0.98
161_N	164_I	0.535	0.98
128_T	154_Y	0.534	0.98
153_H	156_S	0.534	0.98
69_D	215_R	0.534	0.98
182_F	185_G	0.533	0.98
92_G	95_L	0.532	0.98
249_L	282_I	0.532	0.98
249_L	284_Q	0.532	0.98
101_G	180_A	0.528	0.98
13_A	56_N	0.528	0.98
67_A	75_H	0.526	0.98
171_S	174_G	0.526	0.98
132_V	158_V	0.526	0.98
262_M	265_D	0.526	0.98
166_L	170_L	0.523	0.98
143_Q	148_R	0.522	0.98
84_L	205_L	0.517	0.97
202_V	206_L	0.514	0.97
263_V	266_Q	0.514	0.97
39_I	121_T	0.514	0.97
226_P	266_Q	0.513	0.97
15_A	19_M	0.513	0.97
15_A	18_A	0.513	0.97
225_W	228_V	0.512	0.97
220_D	274_R	0.512	0.97
209_A	238_Q	0.512	0.97
22_L	26_I	0.512	0.97
78_A	82_A	0.511	0.97
73_F	235_R	0.509	0.97
221_I	267_V	0.508	0.97
173_Y	176_H	0.506	0.97
187_G	194_A	0.505	0.97
227_G	267_V	0.504	0.97
227_G	255_L	0.504	0.97
272_L	278_S	0.504	0.97
191_L	195_L	0.503	0.97
234_L	245_I	0.502	0.97
128_T	153_H	0.500	0.97

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3j1zP	8	0.98	100	0.093	Contact Map
3h90A	3	0.9433	100	0.125	Contact Map
3bypA	2	0.2667	99.5	0.757	Contact Map
2zztA	2	0.2667	99.4	0.767	Contact Map
3w61A	2	0.2633	99.3	0.785	Contact Map
1ji6A	1	0.42	46.6	0.944	Contact Map
1dlcA	1	0.43	41	0.945	Contact Map
3eb7A	2	0.4233	29.6	0.949	Contact Map
2c9kA	1	0.4233	28.4	0.95	Contact Map
1ib8A	1	0.3233	25	0.951	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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