YGEY - ECOLI - GREMLIN Contacts

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YGEY - Uncharacterized protein YgeY

UniProt:	P65807	Sequences: MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHRIKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGMETDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYIIEQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPILGELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGALDEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEGLFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMYAAIPLSWLATE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13055
Length:	403 (358)
Sequences:	29435 (20847)
Seq/√Len:	1101.8

ECOLI - YGEY DAPE ARGE ABGA PEPT ALLC PEPD ABGB
BACSU - YODQ FAPD PEPT YQJE YHAA YTNL DAPEL YXEP AMHX PEPVL ALLC YQJN ROCB
HALSA - Q9HS25
SULSO - Q97Y12 Q97ZB7 Q97V97 LYSK CBPX1 CBPX2 Q97VB6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
68_Y	141_L	3.428	1.00
193_D	257_S	3.149	1.00
46_K	50_E	2.896	1.00
27_D	44_R	2.826	1.00
193_D	259_A	2.825	1.00
199_C	207_G	2.729	1.00
106_L	379_K	2.710	1.00
75_L	139_T	2.497	1.00
280_R	284_A	2.463	1.00
194_V	217_I	2.462	1.00
276_L	280_R	2.447	1.00
195_Q	257_S	2.439	1.00
191_R	259_A	2.433	1.00
334_G	394_A	2.412	1.00
48_E	51_K	2.388	1.00
330_N	398_S	2.387	1.00
128_I	393_A	2.348	1.00
334_G	398_S	2.345	1.00
195_Q	306_V	2.297	1.00
52_V	127_K	2.268	1.00
214_M	244_I	2.247	1.00
49_M	67_G	2.221	1.00
214_M	258_C	2.215	1.00
44_R	48_E	2.201	1.00
191_R	261_S	2.169	1.00
213_K	303_T	2.165	1.00
68_Y	139_T	2.162	1.00
75_L	141_L	2.157	1.00
277_D	281_A	2.139	1.00
245_F	259_A	2.127	1.00
128_I	132_L	2.039	1.00
214_M	260_V	2.036	1.00
77_A	167_P	2.024	1.00
195_Q	304_G	1.985	1.00
216_P	219_G	1.974	1.00
243_E	261_S	1.970	1.00
20_D	127_K	1.962	1.00
329_V	333_E	1.945	1.00
193_D	308_P	1.939	1.00
31_I	44_R	1.909	1.00
157_Q	161_E	1.899	1.00
215_G	218_L	1.894	1.00
39_K	43_H	1.884	1.00
95_F	103_T	1.814	1.00
48_E	52_V	1.792	1.00
94_D	377_N	1.780	1.00
328_L	395_I	1.767	1.00
47_E	51_K	1.752	1.00
191_R	308_P	1.747	1.00
72_G	137_E	1.734	1.00
217_I	283_P	1.730	1.00
40_R	43_H	1.713	1.00
193_D	306_V	1.712	1.00
43_H	47_E	1.706	1.00
44_R	47_E	1.674	1.00
210_A	258_C	1.659	1.00
197_V	208_D	1.644	1.00
21_M	389_A	1.638	1.00
24_F	48_E	1.638	1.00
105_E	383_S	1.633	1.00
26_R	30_A	1.627	1.00
394_A	397_L	1.610	1.00
208_D	213_K	1.610	1.00
278_E	281_A	1.609	1.00
127_K	131_D	1.597	1.00
215_G	219_G	1.590	1.00
273_E	277_D	1.589	1.00
27_D	40_R	1.587	1.00
158_Y	161_E	1.581	1.00
46_K	65_V	1.576	1.00
54_F	69_I	1.576	1.00
246_F	258_C	1.572	1.00
284_A	290_A	1.568	1.00
23_R	27_D	1.561	1.00
212_F	222_Q	1.558	1.00
333_E	339_A	1.554	1.00
27_D	30_A	1.547	1.00
198_S	256_D	1.540	1.00
203_A	206_R	1.520	1.00
121_S	388_C	1.511	1.00
42_V	82_I	1.499	1.00
220_E	282_L	1.484	1.00
21_M	124_Y	1.476	1.00
77_A	143_T	1.474	1.00
326_K	329_V	1.470	1.00
39_K	63_G	1.468	1.00
19_A	23_R	1.464	1.00
271_T	274_G	1.462	1.00
274_G	278_E	1.459	1.00
74_R	168_E	1.454	1.00
237_G	275_A	1.453	1.00
45_I	82_I	1.452	1.00
47_E	50_E	1.445	1.00
27_D	31_I	1.438	1.00
48_E	127_K	1.437	1.00
327_A	398_S	1.395	1.00
220_E	287_K	1.387	1.00
194_V	214_M	1.383	1.00
394_A	398_S	1.370	1.00
77_A	170_V	1.350	1.00
130_K	133_G	1.348	1.00
30_A	102_E	1.348	1.00
69_I	130_K	1.348	1.00
387_T	390_A	1.346	1.00
56_K	59_I	1.345	1.00
190_I	262_I	1.339	1.00
277_D	280_R	1.319	1.00
22_T	26_R	1.314	1.00
46_K	58_E	1.312	1.00
117_G	385_L	1.311	1.00
191_R	310_E	1.308	1.00
78_M	122_M	1.300	1.00
211_I	246_F	1.292	1.00
23_R	26_R	1.290	1.00
192_I	260_V	1.288	1.00
331_A	398_S	1.285	1.00
329_V	342_V	1.283	1.00
230_Y	278_E	1.277	1.00
80_A	115_Q	1.273	1.00
117_G	380_T	1.263	1.00
235_G	268_W	1.256	1.00
102_E	382_K	1.248	1.00
359_G	362_V	1.241	1.00
332_Y	391_M	1.239	1.00
386_V	390_A	1.238	1.00
33_S	41_V	1.226	1.00
331_A	394_A	1.222	1.00
35_S	150_D	1.220	1.00
78_M	396_P	1.217	1.00
24_F	44_R	1.214	1.00
132_L	393_A	1.212	1.00
24_F	124_Y	1.209	1.00
20_D	23_R	1.209	1.00
276_L	309_T	1.206	1.00
43_H	46_K	1.205	1.00
194_V	213_K	1.203	1.00
383_S	387_T	1.194	1.00
46_K	59_I	1.190	1.00
326_K	330_N	1.186	1.00
211_I	244_I	1.180	1.00
327_A	331_A	1.175	1.00
330_N	334_G	1.162	1.00
281_A	284_A	1.154	1.00
21_M	120_A	1.143	1.00
355_M	360_I	1.138	1.00
132_L	397_L	1.129	1.00
274_G	277_D	1.128	1.00
185_R	188_M	1.128	1.00
28_M	41_V	1.126	1.00
126_G	142_V	1.120	1.00
158_Y	162_Q	1.116	1.00
276_L	311_C	1.115	1.00
29_V	109_G	1.114	1.00
224_L	278_E	1.108	1.00
26_R	102_E	1.106	1.00
268_W	271_T	1.104	1.00
220_E	223_E	1.093	1.00
27_D	48_E	1.093	1.00
381_W	384_H	1.084	1.00
106_L	381_W	1.078	1.00
74_R	138_Y	1.077	1.00
40_R	44_R	1.074	1.00
219_G	223_E	1.073	1.00
26_R	100_G	1.071	1.00
71_H	137_E	1.067	1.00
173_T	392_Y	1.067	1.00
75_L	168_E	1.065	1.00
70_G	73_P	1.061	1.00
287_K	290_A	1.059	1.00
322_H	326_K	1.056	1.00
24_F	127_K	1.052	1.00
45_I	119_M	1.050	1.00
26_R	382_K	1.042	1.00
31_I	40_R	1.039	1.00
281_A	287_K	1.039	1.00
218_L	260_V	1.038	1.00
177_D	373_A	1.035	1.00
95_F	101_M	1.028	1.00
93_W	96_D	1.024	1.00
330_N	333_E	1.022	1.00
245_F	249_P	1.014	1.00
117_G	175_P	1.010	1.00
54_F	67_G	1.010	1.00
32_P	37_D	1.008	1.00
69_I	136_D	1.005	1.00
29_V	112_T	1.003	1.00
42_V	65_V	1.000	1.00
190_I	276_L	0.999	1.00
212_F	218_L	0.996	1.00
50_E	56_K	0.996	1.00
241_V	262_I	0.996	1.00
180_V	183_G	0.991	1.00
105_E	381_W	0.985	1.00
95_F	98_Y	0.984	1.00
278_E	282_L	0.982	1.00
124_Y	127_K	0.978	1.00
284_A	289_N	0.977	1.00
49_M	123_V	0.974	1.00
122_M	142_V	0.974	1.00
289_N	305_L	0.973	1.00
64_N	146_V	0.969	1.00
214_M	218_L	0.964	1.00
393_A	397_L	0.963	1.00
328_L	363_I	0.961	1.00
20_D	24_F	0.959	1.00
216_P	220_E	0.958	1.00
82_I	144_G	0.958	1.00
28_M	44_R	0.954	1.00
216_P	223_E	0.952	1.00
218_L	241_V	0.948	1.00
250_S	253_A	0.945	1.00
389_A	393_A	0.943	1.00
42_V	46_K	0.942	1.00
169_F	324_T	0.940	1.00
125_A	393_A	0.939	1.00
331_A	334_G	0.938	1.00
321_D	324_T	0.937	1.00
181_Y	184_Q	0.936	1.00
192_I	283_P	0.936	1.00
380_T	385_L	0.933	1.00
21_M	385_L	0.930	1.00
390_A	394_A	0.929	1.00
128_I	390_A	0.925	1.00
327_A	330_N	0.925	1.00
20_D	48_E	0.925	1.00
57_V	68_Y	0.923	1.00
178_C	183_G	0.921	1.00
36_C	147_Q	0.919	1.00
280_R	307_Y	0.919	1.00
80_A	119_M	0.913	1.00
360_I	366_G	0.912	1.00
195_Q	256_D	0.910	1.00
55_D	68_Y	0.909	1.00
184_Q	188_M	0.906	1.00
191_R	251_R	0.905	1.00
70_G	139_T	0.901	1.00
251_R	261_S	0.896	1.00
37_D	40_R	0.895	1.00
384_H	387_T	0.892	1.00
57_V	60_D	0.890	1.00
82_I	119_M	0.888	1.00
283_P	305_L	0.885	1.00
54_F	126_G	0.883	1.00
125_A	392_Y	0.877	1.00
221_L	279_I	0.876	1.00
48_E	123_V	0.873	1.00
327_A	395_I	0.873	1.00
52_V	130_K	0.870	1.00
88_G	104_D	0.868	1.00
103_T	106_L	0.865	1.00
359_G	366_G	0.864	1.00
71_H	135_E	0.863	1.00
333_E	338_K	0.860	1.00
192_I	217_I	0.858	1.00
323_F	327_A	0.857	1.00
212_F	215_G	0.855	1.00
282_L	287_K	0.854	1.00
216_P	222_Q	0.852	1.00
211_I	258_C	0.851	1.00
218_L	244_I	0.850	1.00
362_V	365_F	0.849	1.00
323_F	326_K	0.849	1.00
69_I	126_G	0.847	1.00
179_Q	183_G	0.843	1.00
27_D	47_E	0.840	1.00
372_E	375_A	0.839	1.00
180_V	184_Q	0.838	1.00
269_G	273_E	0.835	1.00
355_M	358_H	0.834	1.00
273_E	276_L	0.832	1.00
46_K	56_K	0.832	1.00
325_V	329_V	0.830	1.00
141_L	167_P	0.827	1.00
103_T	108_G	0.823	1.00
266_L	272_W	0.819	1.00
87_I	98_Y	0.818	1.00
281_A	290_A	0.817	1.00
41_V	82_I	0.810	1.00
244_I	258_C	0.810	1.00
371_P	377_N	0.810	1.00
282_L	288_A	0.807	1.00
102_E	105_E	0.806	1.00
156_W	160_I	0.805	1.00
91_K	95_F	0.804	1.00
383_S	386_V	0.802	1.00
132_L	135_E	0.802	1.00
208_D	303_T	0.801	1.00
188_M	272_W	0.801	1.00
76_V	169_F	0.799	1.00
101_M	104_D	0.797	1.00
52_V	123_V	0.796	1.00
181_Y	185_R	0.795	1.00
178_C	182_R	0.794	1.00
336_F	394_A	0.793	1.00
171_V	395_I	0.791	1.00
115_Q	118_G	0.789	1.00
50_E	58_E	0.789	1.00
66_L	141_L	0.785	1.00
183_G	344_K	0.785	1.00
43_H	58_E	0.782	1.00
128_I	131_D	0.782	1.00
30_A	100_G	0.780	1.00
270_E	274_G	0.780	1.00
212_F	223_E	0.779	1.00
94_D	103_T	0.779	1.00
78_M	125_A	0.776	1.00
54_F	130_K	0.776	1.00
24_F	120_A	0.775	1.00
46_K	57_V	0.766	1.00
92_N	95_F	0.766	1.00
33_S	116_E	0.763	1.00
25_L	107_I	0.762	1.00
126_G	130_K	0.761	1.00
121_S	389_A	0.760	1.00
97_P	100_G	0.759	1.00
51_K	127_K	0.757	1.00
121_S	392_Y	0.757	1.00
219_G	222_Q	0.756	1.00
362_V	366_G	0.756	1.00
24_F	27_D	0.754	1.00
125_A	389_A	0.751	1.00
95_F	100_G	0.749	1.00
93_W	97_P	0.748	1.00
128_I	389_A	0.746	1.00
328_L	391_M	0.744	1.00
154_L	158_Y	0.739	1.00
22_T	102_E	0.736	1.00
264_R	275_A	0.735	1.00
385_L	389_A	0.735	1.00
241_V	260_V	0.735	1.00
221_L	239_L	0.732	1.00
213_K	305_L	0.729	1.00
220_E	288_A	0.727	1.00
313_F	316_W	0.725	1.00
332_Y	336_F	0.725	1.00
127_K	130_K	0.723	1.00
100_G	107_I	0.723	1.00
141_L	166_R	0.722	1.00
124_Y	389_A	0.721	1.00
361_P	366_G	0.721	1.00
121_S	125_A	0.718	1.00
92_N	96_D	0.716	1.00
75_L	138_Y	0.716	1.00
170_V	360_I	0.716	1.00
188_M	276_L	0.714	1.00
272_W	314_P	0.711	1.00
194_V	260_V	0.709	1.00
272_W	276_L	0.709	1.00
336_F	391_M	0.709	1.00
26_R	29_V	0.707	1.00
94_D	97_P	0.706	1.00
34_E	86_G	0.705	1.00
331_A	391_M	0.702	1.00
184_Q	187_R	0.701	1.00
360_I	365_F	0.696	1.00
178_C	185_R	0.693	1.00
122_M	173_T	0.693	1.00
65_V	144_G	0.691	1.00
217_I	260_V	0.690	1.00
360_I	363_I	0.690	1.00
45_I	123_V	0.688	1.00
45_I	144_G	0.687	1.00
197_V	375_A	0.687	1.00
189_E	263_D	0.685	1.00
84_T	98_Y	0.685	1.00
42_V	63_G	0.683	1.00
76_V	396_P	0.681	1.00
245_F	248_S	0.679	1.00
311_C	314_P	0.679	1.00
239_L	264_R	0.678	1.00
368_G	380_T	0.676	1.00
24_F	28_M	0.676	1.00
225_S	239_L	0.675	1.00
180_V	185_R	0.673	1.00
54_F	57_V	0.672	1.00
331_A	395_I	0.670	1.00
351_G	362_V	0.668	1.00
73_P	139_T	0.668	1.00
45_I	65_V	0.668	1.00
199_C	254_V	0.664	1.00
36_C	150_D	0.662	1.00
50_E	55_D	0.662	1.00
34_E	98_Y	0.661	1.00
69_I	142_V	0.660	1.00
119_M	144_G	0.659	1.00
273_E	311_C	0.657	1.00
34_E	37_D	0.657	1.00
372_E	377_N	0.655	1.00
174_E	177_D	0.653	1.00
190_I	279_I	0.653	1.00
390_A	393_A	0.651	1.00
20_D	124_Y	0.650	1.00
56_K	67_G	0.649	1.00
205_E	232_E	0.649	1.00
186_G	266_L	0.644	1.00
22_T	382_K	0.642	1.00
280_R	309_T	0.642	1.00
171_V	324_T	0.642	1.00
28_M	119_M	0.641	1.00
143_T	159_I	0.637	1.00
391_M	395_I	0.636	1.00
209_N	255_A	0.632	1.00
189_E	251_R	0.631	1.00
371_P	375_A	0.628	1.00
172_S	351_G	0.625	1.00
363_I	366_G	0.624	1.00
58_E	164_G	0.622	1.00
72_G	139_T	0.622	1.00
57_V	164_G	0.621	1.00
26_R	107_I	0.620	1.00
309_T	312_Y	0.620	1.00
182_R	185_R	0.619	1.00
321_D	326_K	0.617	1.00
231_D	237_G	0.615	1.00
149_E	374_H	0.614	1.00
182_R	342_V	0.612	1.00
212_F	216_P	0.611	1.00
382_K	386_V	0.609	1.00
107_I	382_K	0.608	1.00
22_T	107_I	0.608	1.00
266_L	275_A	0.605	1.00
210_A	255_A	0.603	1.00
129_I	134_L	0.603	1.00
147_Q	150_D	0.603	1.00
194_V	210_A	0.602	1.00
64_N	159_I	0.602	1.00
138_Y	168_E	0.601	1.00
245_F	261_S	0.600	1.00
84_T	110_R	0.597	1.00
121_S	173_T	0.597	1.00
227_R	278_E	0.596	1.00
54_F	142_V	0.595	1.00
150_D	154_L	0.593	1.00
62_M	158_Y	0.593	1.00
172_S	175_P	0.592	1.00
212_F	219_G	0.592	1.00
277_D	284_A	0.592	1.00
79_D	143_T	0.589	1.00
329_V	341_V	0.588	1.00
208_D	304_G	0.587	1.00
108_G	379_K	0.585	1.00
172_S	362_V	0.585	1.00
252_C	265_R	0.584	1.00
243_E	259_A	0.584	1.00
24_F	123_V	0.584	1.00
40_R	47_E	0.582	1.00
322_H	361_P	0.582	1.00
179_Q	343_D	0.581	1.00
217_I	282_L	0.581	1.00
80_A	122_M	0.581	1.00
370_E	373_A	0.580	1.00
201_G	240_T	0.580	1.00
359_G	363_I	0.579	1.00
201_G	242_S	0.579	1.00
372_E	379_K	0.576	1.00
96_D	100_G	0.575	1.00
34_E	106_L	0.575	1.00
179_Q	341_V	0.575	1.00
28_M	120_A	0.571	1.00
364_G	368_G	0.570	1.00
157_Q	160_I	0.568	1.00
194_V	305_L	0.568	1.00
184_Q	349_T	0.568	1.00
262_I	279_I	0.568	1.00
325_V	361_P	0.565	1.00
78_M	171_V	0.565	1.00
243_E	248_S	0.564	1.00
38_E	147_Q	0.564	1.00
232_E	235_G	0.564	1.00
31_I	103_T	0.562	1.00
87_I	109_G	0.560	1.00
42_V	59_I	0.560	1.00
245_F	257_S	0.559	1.00
120_A	124_Y	0.559	1.00
66_L	164_G	0.558	1.00
180_V	187_R	0.557	1.00
181_Y	188_M	0.557	1.00
66_L	143_T	0.557	1.00
25_L	120_A	0.555	1.00
49_M	54_F	0.554	1.00
124_Y	128_I	0.552	1.00
172_S	177_D	0.551	1.00
363_I	367_P	0.550	1.00
324_T	327_A	0.548	1.00
75_L	166_R	0.548	1.00
94_D	98_Y	0.546	1.00
314_P	357_R	0.546	1.00
284_A	287_K	0.545	1.00
208_D	302_W	0.545	1.00
37_D	106_L	0.545	1.00
79_D	145_T	0.544	1.00
192_I	307_Y	0.543	1.00
160_I	163_S	0.542	1.00
213_K	216_P	0.542	1.00
221_L	241_V	0.542	1.00
22_T	386_V	0.539	1.00
175_P	367_P	0.538	1.00
243_E	249_P	0.534	1.00
224_L	227_R	0.534	1.00
102_E	107_I	0.533	1.00
25_L	382_K	0.530	1.00
179_Q	184_Q	0.529	1.00
264_R	279_I	0.529	1.00
215_G	222_Q	0.526	1.00
31_I	41_V	0.526	1.00
115_Q	119_M	0.525	1.00
269_G	356_G	0.523	1.00
369_K	379_K	0.521	1.00
154_L	157_Q	0.521	1.00
36_C	40_R	0.519	1.00
210_A	254_V	0.517	1.00
143_T	170_V	0.517	1.00
181_Y	343_D	0.516	1.00
192_I	262_I	0.515	1.00
167_P	170_V	0.515	1.00
171_V	174_E	0.514	1.00
386_V	389_A	0.513	1.00
178_C	184_Q	0.512	1.00
20_D	51_K	0.511	1.00
68_Y	75_L	0.511	1.00
329_V	340_P	0.511	1.00
24_F	52_V	0.511	1.00
49_M	126_G	0.509	1.00
283_P	307_Y	0.509	1.00
336_F	387_T	0.509	1.00
21_M	25_L	0.508	1.00
133_G	136_D	0.508	1.00
232_E	343_D	0.507	1.00
192_I	279_I	0.506	1.00
34_E	87_I	0.505	1.00
76_V	171_V	0.505	1.00
19_A	22_T	0.504	1.00
244_I	260_V	0.504	1.00
190_I	309_T	0.503	1.00
91_K	94_D	0.502	1.00
198_S	376_P	0.500	1.00
217_I	221_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3pfoA	2	0.9653	100	0.197	Contact Map
3iszA	2	0.8933	100	0.244	Contact Map
4pqaA	2	0.9057	100	0.248	Contact Map
4mmoA	2	0.9132	100	0.264	Contact Map
1cg2A	4	0.9231	100	0.264	Contact Map
4g1pA	2	0.9677	100	0.266	Contact Map
2pokA	2	0.9529	100	0.271	Contact Map
3rzaA	2	0.8834	100	0.274	Contact Map
4q7aA	5	0.8809	100	0.274	Contact Map
3dljA	2	0.9653	100	0.276	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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