GREMLIN Database
HIGB - mRNA interferase HigB
UniProt: P64578 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12725
Length: 104 (93)
Sequences: 745 (563)
Seq/√Len: 58.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
63_G66_E3.9631.00
20_K24_V2.8641.00
2_H79_K2.7281.00
62_V69_V2.6121.00
58_Y75_F2.4141.00
34_Y78_Q2.2401.00
51_F54_L2.1611.00
73_V80_C1.9061.00
30_I80_C1.8821.00
30_I35_F1.8741.00
86_M94_F1.7800.99
6_Q27_G1.7660.99
29_T45_F1.7300.99
12_A85_V1.6780.99
37_K40_S1.6340.99
13_A24_V1.6180.99
57_H94_F1.5820.99
5_T8_A1.5740.98
12_A67_L1.5490.98
36_K78_Q1.5290.98
70_V94_F1.5150.98
49_D61_N1.4520.97
42_K49_D1.4460.97
74_F83_R1.4230.97
74_F77_S1.4170.97
72_M83_R1.3590.96
13_A20_K1.3530.96
84_E94_F1.3420.96
48_L58_Y1.3340.96
3_L27_G1.3240.96
57_H86_M1.3150.95
7_K11_D1.2850.95
74_F81_Y1.2720.94
16_Y85_V1.2660.94
23_L62_V1.2220.93
48_L61_N1.1800.92
25_A29_T1.1780.92
26_L60_F1.1750.92
10_K14_E1.1660.91
10_K13_A1.1650.91
3_L31_A1.1490.91
2_H81_Y1.1450.91
19_H67_L1.0800.88
6_Q24_V1.0510.86
30_I41_L1.0300.85
29_T44_V1.0090.84
57_H83_R1.0050.84
38_P75_F0.9880.83
57_H84_E0.9800.82
23_L85_V0.9790.82
22_E64_G0.9760.82
9_L85_V0.9650.81
37_K41_L0.9520.80
38_P50_N0.9510.80
15_K36_K0.9490.80
21_T24_V0.9320.79
61_N69_V0.9190.78
42_K47_S0.9150.78
72_M81_Y0.9110.77
18_Q67_L0.9010.77
3_L30_I0.8940.76
12_A16_Y0.8850.75
49_D58_Y0.8820.75
42_K61_N0.8740.74
18_Q66_E0.8590.73
89_K93_F0.8560.73
10_K24_V0.8550.73
69_V85_V0.8520.73
22_E63_G0.8280.70
17_P90_E0.8160.69
87_T90_E0.8160.69
71_A80_C0.8100.69
30_I33_G0.8030.68
49_D59_V0.7950.67
9_L13_A0.7890.67
56_K75_F0.7870.66
50_N58_Y0.7810.66
70_V86_M0.7800.66
19_H66_E0.7730.65
72_M77_S0.7660.64
41_L73_V0.7610.64
3_L21_T0.7440.62
2_H78_Q0.7380.61
46_P90_E0.7340.61
18_Q61_N0.7270.60
4_I74_F0.7210.60
3_L82_I0.7160.59
2_H83_R0.7090.58
51_F59_V0.7080.58
39_E93_F0.6960.57
5_T83_R0.6950.57
9_L67_L0.6940.57
12_A87_T0.6770.55
79_K83_R0.6720.54
33_G44_V0.6700.54
47_S61_N0.6670.54
59_V81_Y0.6600.53
19_H22_E0.6570.53
72_M79_K0.6560.53
19_H60_F0.6500.52
27_G31_A0.6480.52
2_H72_M0.6350.50
32_K95_T0.6280.49
17_P93_F0.6270.49
43_A89_K0.6240.49
15_K20_K0.6240.49
37_K90_E0.6210.49
45_F49_D0.6150.48
86_M95_T0.6140.48
38_P42_K0.6070.47
2_H5_T0.6070.47
41_L50_N0.5950.46
11_D14_E0.5830.45
12_A19_H0.5810.44
5_T81_Y0.5790.44
6_Q10_K0.5770.44
2_H38_P0.5740.44
38_P58_Y0.5730.43
42_K64_G0.5640.42
36_K40_S0.5580.42
6_Q13_A0.5490.41
6_Q39_E0.5480.41
28_N76_E0.5420.40
55_D77_S0.5380.40
43_A46_P0.5380.40
16_Y87_T0.5340.39
8_A75_F0.5290.39
43_A66_E0.5290.39
15_K89_K0.5240.38
51_F73_V0.5210.38
61_N65_N0.5210.38
29_T49_D0.5160.37
21_T25_A0.5150.37
23_L69_V0.5130.37
6_Q72_M0.5130.37
28_N77_S0.5100.37
46_P49_D0.5050.36
74_F78_Q0.5050.36
35_F41_L0.5030.36
38_P51_F0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3bpqB 1 0.7981 68.6 0.901 Contact Map
4nrnA 2 0.7885 62.3 0.905 Contact Map
3g5oB 1 0.7308 58.8 0.907 Contact Map
4lttA 1 0.7981 53.3 0.91 Contact Map
3oeiC 2 0.7404 42.4 0.915 Contact Map
1wmiA 2 0.7692 38.6 0.917 Contact Map
2kheA 1 0.7885 36.8 0.918 Contact Map
4ml2A 1 0.7692 35.6 0.919 Contact Map
2a6sA 1 0.7404 32.1 0.921 Contact Map
4mcxB 2 0.7596 25.6 0.924 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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