GREMLIN Database
ALAE - L-alanine exporter AlaE
UniProt: P64550 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13525
Length: 149 (136)
Sequences: 101 (46)
Seq/√Len: 3.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
90_T126_A2.9800.60
61_M86_L2.8940.57
66_F86_L2.8510.56
21_Y122_M2.7930.54
25_V47_V2.5490.47
23_S48_A2.4960.46
21_Y134_Y2.4620.45
100_L116_S2.2880.40
21_Y43_Y2.2080.38
85_I126_A2.1160.35
115_S133_D2.0870.35
125_G128_Y2.0060.32
32_F74_S2.0020.32
39_E82_L1.9840.32
51_V113_A1.9130.30
54_L66_F1.8460.29
102_V119_V1.7880.27
26_N48_A1.7660.27
62_Y90_T1.7200.26
21_Y127_V1.7000.25
22_C98_A1.6780.25
76_S90_T1.6690.25
106_D138_L1.6450.24
46_L62_Y1.6270.24
12_V32_F1.6030.23
85_I90_T1.5960.23
68_R86_L1.5850.23
7_R83_A1.5300.22
61_M75_P1.4800.21
36_M53_I1.4710.21
28_C32_F1.4460.20
37_S119_V1.4340.20
12_V103_V1.4330.20
50_P87_A1.4040.19
41_S52_N1.4000.19
119_V131_F1.3990.19
13_A128_Y1.3940.19
25_V118_I1.3910.19
84_D129_G1.3480.18
98_A123_L1.2920.17
18_M128_Y1.2900.17
13_A17_A1.2890.17
103_V120_V1.2610.17
21_Y62_Y1.2590.17
22_C123_L1.2580.17
82_L86_L1.2570.17
47_V126_A1.2540.17
57_W126_A1.2320.16
32_F39_E1.2140.16
7_R56_A1.2130.16
13_A60_G1.2130.16
24_V32_F1.1850.16
85_I130_Y1.1790.16
46_L138_L1.1770.16
81_N85_I1.1550.15
99_I132_L1.1550.15
10_H56_A1.1390.15
57_W127_V1.1380.15
46_L90_T1.1180.15
13_A125_G1.1170.15
70_A83_A1.1120.15
37_S142_S1.1060.15
54_L70_A1.0930.14
68_R78_W1.0900.14
16_F135_C1.0660.14
33_L95_V1.0520.14
27_M41_S1.0480.14
36_M136_R1.0440.14
111_M119_V1.0390.14
60_G64_D1.0280.13
26_N40_Q1.0270.13
54_L142_S1.0210.13
53_I89_V1.0070.13
102_V127_V1.0070.13
17_A125_G0.9990.13
25_V54_L0.9910.13
47_V115_S0.9790.13
22_C81_N0.9680.13
82_L106_D0.9570.13
24_V107_W0.9380.12
67_M70_A0.9350.12
122_M134_Y0.9340.12
88_Y100_L0.9220.12
37_S81_N0.9210.12
119_V137_R0.9140.12
52_N140_K0.9130.12
90_T108_H0.9100.12
32_F80_K0.8900.12
86_L110_I0.8880.12
98_A101_L0.8800.12
102_V142_S0.8770.12
108_H142_S0.8660.11
84_D136_R0.8650.11
129_G136_R0.8650.11
21_Y138_L0.8640.11
13_A44_S0.8400.11
28_C33_L0.8310.11
54_L83_A0.8170.11
21_Y130_Y0.8160.11
44_S117_N0.8150.11
29_I113_A0.8090.11
29_I36_M0.8060.11
25_V37_S0.7960.11
23_S53_I0.7950.11
49_I56_A0.7930.11
38_F100_L0.7930.11
52_N62_Y0.7900.11
33_L78_W0.7850.11
49_I113_A0.7820.11
17_A128_Y0.7820.11
41_S117_N0.7800.10
25_V106_D0.7780.10
64_D87_A0.7780.10
23_S126_A0.7770.10
22_C130_Y0.7760.10
115_S123_L0.7740.10
100_L141_V0.7680.10
58_P104_G0.7650.10
9_R95_V0.7600.10
127_V142_S0.7590.10
13_A26_N0.7590.10
85_I120_V0.7540.10
67_M90_T0.7530.10
69_A82_L0.7520.10
74_S120_V0.7470.10
29_I88_Y0.7470.10
122_M127_V0.7380.10
29_I52_N0.7330.10
28_C85_I0.7190.10
35_G92_Q0.7140.10
123_L138_L0.7130.10
7_R106_D0.7130.10
16_F102_V0.7100.10
8_L80_K0.7090.10
82_L95_V0.7050.10
51_V100_L0.7050.10
7_R37_S0.6910.10
48_A62_Y0.6890.10
27_M135_C0.6860.10
51_V95_V0.6860.10
111_M115_S0.6810.09
10_H18_M0.6800.09
39_E108_H0.6800.09
112_A142_S0.6760.09
47_V62_Y0.6740.09
54_L79_I0.6680.09
68_R77_G0.6670.09
66_F142_S0.6620.09
82_L123_L0.6620.09
23_S26_N0.6610.09
18_M115_S0.6510.09
65_L102_V0.6500.09
67_M115_S0.6480.09
98_A111_M0.6470.09
28_C81_N0.6410.09
31_V116_S0.6300.09
16_F56_A0.6300.09
43_Y122_M0.6280.09
31_V88_Y0.6270.09
106_D123_L0.6240.09
18_M38_F0.6230.09
8_L21_Y0.6210.09
66_F101_L0.6140.09
56_A89_V0.6100.09
66_F97_V0.6100.09
78_W127_V0.6090.09
57_W81_N0.6070.09
7_R21_Y0.6060.09
107_W111_M0.5990.09
43_Y130_Y0.5960.09
34_S57_W0.5920.09
9_R114_V0.5860.09
67_M76_S0.5820.09
43_Y46_L0.5740.08
101_L106_D0.5730.08
12_V98_A0.5660.08
79_I98_A0.5610.08
101_L141_V0.5600.08
7_R138_L0.5540.08
29_I119_V0.5520.08
59_Y105_A0.5480.08
78_W107_W0.5480.08
47_V108_H0.5450.08
79_I86_L0.5430.08
35_G40_Q0.5410.08
19_V117_N0.5310.08
51_V88_Y0.5270.08
66_F106_D0.5260.08
32_F73_V0.5260.08
12_V46_L0.5230.08
61_M69_A0.5210.08
32_F142_S0.5200.08
7_R70_A0.5200.08
34_S127_V0.5190.08
12_V79_I0.5180.08
15_T122_M0.5180.08
40_Q114_V0.5140.08
9_R135_C0.5130.08
47_V124_M0.5120.08
46_L54_L0.5110.08
37_S137_R0.5060.08
55_I103_V0.5060.08
90_T115_S0.5020.08
33_L75_P0.5000.08
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4dveA 3 0.4832 10.9 0.962 Contact Map
4cz8A 2 0.8456 2.9 0.971 Contact Map
1i5pA 1 0.6309 2.9 0.971 Contact Map
4xp4A 1 0.8926 2.4 0.972 Contact Map
2qmiA 5 0.9597 2.4 0.972 Contact Map
1b35D 3 0.1409 2.3 0.972 Contact Map
4huqS 1 0.4899 2 0.973 Contact Map
3sbxA 2 0.6174 1.8 0.974 Contact Map
1bgkA 1 0.1074 1.5 0.975 Contact Map
3g14A 2 0.7047 1.4 0.975 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0063 seconds.