GREMLIN Database
ISPH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
UniProt: P62623 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11081
Length: 316 (283)
Sequences: 2958 (1614)
Seq/√Len: 96.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
2_Q261_C4.0351.00
115_E142_Y3.4721.00
150_V180_A3.3441.00
114_E160_K3.0811.00
248_A277_N3.0151.00
82_N86_S2.9871.00
131_M143_L2.8741.00
216_V259_V2.8031.00
219_V265_T2.7431.00
47_D50_R2.7251.00
4_L213_Q2.6951.00
147_P176_D2.6431.00
44_Y247_D2.5101.00
217_V235_A2.4361.00
108_R112_R2.3611.00
106_V164_M2.3181.00
147_P177_V2.2931.00
145_E149_D2.2851.00
47_D51_E2.2571.00
109_A114_E2.1961.00
151_W180_A2.1211.00
61_S83_E2.1011.00
251_I278_V2.0881.00
82_N85_K2.0661.00
107_A111_R2.0211.00
151_W183_K2.0101.00
68_I90_T1.9931.00
243_F255_W1.9741.00
281_R285_L1.9181.00
207_V235_A1.9081.00
241_R259_V1.9051.00
232_A236_Q1.9011.00
4_L261_C1.8961.00
26_L54_A1.8601.00
48_S52_R1.8491.00
83_E86_S1.8351.00
222_S246_D1.8191.00
24_N27_A1.8121.00
25_A92_F1.8121.00
109_A162_S1.8021.00
248_A281_R1.7851.00
253_E285_L1.7771.00
23_E48_S1.7701.00
216_V243_F1.7671.00
209_A213_Q1.7321.00
48_S273_I1.7231.00
214_A217_V1.7141.00
180_A183_K1.7141.00
256_V262_V1.6901.00
181_L188_I1.6871.00
83_E87_R1.6861.00
252_Q255_W1.6661.00
218_L245_I1.6401.00
50_R55_I1.6271.00
211_A238_M1.6161.00
214_A263_G1.6051.00
77_S132_G1.5931.00
43_R47_D1.5721.00
245_I251_I1.5701.00
281_R284_Q1.5661.00
159_E187_K1.5621.00
228_S244_L1.5401.00
146_S149_D1.5091.00
78_Q107_A1.5081.00
27_A52_R1.5011.00
23_E52_R1.4821.00
201_T205_E1.4671.00
160_K187_K1.4651.00
115_E156_K1.4501.00
57_I63_V1.4281.00
148_D151_W1.4241.00
78_Q132_G1.4241.00
247_D250_D1.3970.99
59_Q62_E1.3900.99
8_P206_A1.3900.99
150_V153_L1.3890.99
218_L251_I1.3870.99
106_V130_T1.3840.99
65_D88_D1.3740.99
223_K250_D1.3740.99
220_V278_V1.3610.99
212_E238_M1.3590.99
254_E257_K1.3470.99
3_I279_V1.3390.99
131_M141_M1.3250.99
20_S24_N1.3180.99
22_V70_I1.3140.99
119_I177_V1.3110.99
235_A242_A1.3100.99
216_V256_V1.3050.99
48_S51_E1.2970.99
84_A89_L1.2960.99
115_E140_G1.2620.99
236_Q241_R1.2550.99
4_L214_A1.2550.99
147_P180_A1.2460.99
215_E259_V1.2450.99
13_A98_L1.2380.99
214_A261_C1.2350.99
122_A149_D1.2230.99
172_D208_R1.2200.98
3_I264_V1.2160.98
45_V48_S1.1990.98
32_P55_I1.1830.98
217_V263_G1.1820.98
209_A212_E1.1800.98
150_V177_V1.1780.98
157_N160_K1.1670.98
155_V158_E1.1640.98
144_V153_L1.1560.98
117_I144_V1.1510.98
109_A112_R1.1400.98
36_R80_V1.1350.98
22_V54_A1.1290.97
118_L164_M1.1280.97
264_V279_V1.1080.97
199_A268_A1.1020.97
5_L279_V1.0930.97
44_Y273_I1.0900.97
121_H226_S1.0880.97
109_A164_M1.0870.97
35_V38_E1.0850.97
76_V80_V1.0840.97
104_M107_A1.0810.97
6_A210_L1.0810.97
24_N28_I1.0810.97
179_D183_K1.0800.97
43_R222_S1.0770.97
259_V262_V1.0740.97
249_K277_N1.0580.96
121_H230_R1.0570.96
22_V35_V1.0510.96
223_K244_L1.0430.96
207_V265_T1.0360.96
251_I256_V1.0320.96
78_Q82_N1.0320.96
123_G128_E1.0300.96
43_R246_D1.0270.96
230_R233_E1.0270.96
228_S267_G1.0240.96
180_A184_R1.0240.96
118_L127_V1.0210.95
2_Q289_E1.0200.95
216_V255_W1.0150.95
38_E224_N1.0130.95
163_F178_I1.0100.95
205_E209_A1.0080.95
78_Q133_Q1.0050.95
129_G132_G1.0040.95
35_V54_A1.0010.95
154_T158_E0.9990.95
215_E260_K0.9970.95
17_R21_I0.9840.94
216_V262_V0.9800.94
135_S140_G0.9800.94
175_S179_D0.9720.94
146_S150_V0.9720.94
110_S134_Y0.9690.94
135_S139_G0.9660.94
110_S140_G0.9630.94
7_N209_A0.9510.93
103_H130_T0.9500.93
207_V217_V0.9480.93
69_L84_A0.9480.93
60_I84_A0.9460.93
119_I163_F0.9420.93
218_L255_W0.9350.93
9_R206_A0.9310.93
117_I153_L0.9310.93
114_E189_V0.9270.92
272_D276_Q0.9250.92
31_A68_I0.9240.92
218_L256_V0.9190.92
208_R237_R0.9140.92
165_T174_T0.9070.92
122_A145_E0.9060.92
2_Q260_K0.9010.91
37_H72_S0.8980.91
229_N232_A0.8900.91
38_E50_R0.8880.91
256_V259_V0.8880.91
7_N206_A0.8870.91
101_K105_E0.8820.90
37_H125_P0.8810.90
208_R234_L0.8750.90
81_R133_Q0.8730.90
61_S87_R0.8600.89
280_A283_Q0.8580.89
199_A206_A0.8570.89
253_E281_R0.8540.89
266_A275_V0.8500.89
247_D277_N0.8470.89
179_D182_R0.8460.89
280_A284_Q0.8420.88
78_Q81_R0.8410.88
79_A82_N0.8400.88
29_Y68_I0.8370.88
79_A83_E0.8310.88
232_A242_A0.8300.88
199_A265_T0.8270.87
279_V283_Q0.8260.87
23_E49_L0.8240.87
26_L52_R0.8190.87
169_L173_D0.8170.87
65_D87_R0.8170.87
21_I24_N0.8130.87
77_S81_R0.8090.86
64_P87_R0.8090.86
252_Q256_V0.8070.86
19_I45_V0.8030.86
44_Y47_D0.8030.86
64_P67_A0.7970.85
218_L243_F0.7940.85
115_E159_E0.7930.85
115_E153_L0.7900.85
134_Y139_G0.7880.85
251_I281_R0.7870.85
277_N282_L0.7860.85
142_Y153_L0.7820.84
106_V116_S0.7760.84
39_V194_D0.7700.83
151_W184_R0.7690.83
169_L226_S0.7680.83
148_D152_K0.7660.83
116_S162_S0.7650.83
221_G267_G0.7630.83
81_R93_D0.7610.83
220_V245_I0.7590.83
158_E187_K0.7560.82
33_I54_A0.7550.82
105_E193_K0.7520.82
77_S133_Q0.7510.82
60_I69_L0.7510.82
244_L251_I0.7510.82
161_L188_I0.7490.82
114_E162_S0.7470.81
22_V49_L0.7460.81
111_R135_S0.7460.81
40_V73_A0.7450.81
221_G228_S0.7450.81
282_L286_G0.7430.81
16_D20_S0.7420.81
223_K246_D0.7370.81
123_G143_L0.7360.81
98_L230_R0.7340.80
77_S128_E0.7330.80
39_V92_F0.7290.80
110_S135_S0.7280.80
19_I49_L0.7280.80
226_S230_R0.7280.80
11_F19_I0.7260.80
116_S164_M0.7250.80
105_E162_S0.7250.80
98_L198_Y0.7240.79
63_V69_L0.7240.79
118_L141_M0.7220.79
236_Q240_K0.7160.79
232_A244_L0.7130.78
58_E62_E0.7130.78
200_T204_Q0.7100.78
69_L89_L0.7100.78
110_S139_G0.7070.78
163_F181_L0.7060.78
102_V226_S0.7020.77
102_V130_T0.6960.77
147_P151_W0.6950.77
81_R100_T0.6920.76
118_L143_L0.6900.76
66_G89_L0.6890.76
112_R160_K0.6860.76
220_V264_V0.6850.76
176_D179_D0.6850.76
216_V239_G0.6850.76
5_L264_V0.6840.75
63_V66_G0.6840.75
264_V278_V0.6810.75
131_M139_G0.6810.75
44_Y222_S0.6790.75
235_A240_K0.6780.75
7_N39_V0.6780.75
117_I161_L0.6780.75
25_A70_I0.6770.75
115_E141_M0.6750.75
112_R189_V0.6750.75
13_A121_H0.6730.74
134_Y140_G0.6700.74
77_S91_V0.6670.74
43_R224_N0.6660.74
110_S116_S0.6650.73
22_V39_V0.6640.73
6_A263_G0.6630.73
3_I283_Q0.6630.73
222_S228_S0.6590.73
185_F188_I0.6500.72
39_V46_V0.6470.71
85_K88_D0.6460.71
110_S118_L0.6450.71
103_H129_G0.6400.71
98_L103_H0.6390.70
199_A204_Q0.6390.70
29_Y92_F0.6390.70
173_D177_V0.6340.70
171_V194_D0.6320.70
8_P202_N0.6300.69
106_V134_Y0.6300.69
63_V275_V0.6290.69
118_L131_M0.6280.69
253_E256_V0.6260.69
6_A16_D0.6260.69
148_D249_K0.6250.69
26_L33_I0.6250.69
10_G14_G0.6240.69
149_D152_K0.6230.69
165_T169_L0.6220.68
144_V149_D0.6220.68
10_G15_V0.6170.68
123_G145_E0.6170.68
71_F84_A0.6160.68
245_I278_V0.6160.68
208_R212_E0.6150.68
63_V67_A0.6140.67
35_V56_F0.6140.67
60_I83_E0.6140.67
109_A160_K0.6140.67
104_M108_R0.6070.67
73_A133_Q0.6050.66
12_C15_V0.6040.66
206_A272_D0.6020.66
150_V181_L0.6000.66
156_K159_E0.6000.66
218_L244_L0.5980.65
102_V167_T0.5970.65
57_I64_P0.5960.65
208_R238_M0.5960.65
216_V252_Q0.5940.65
3_I262_V0.5940.65
264_V286_G0.5920.65
76_V81_R0.5900.64
34_Y55_I0.5890.64
198_Y202_N0.5880.64
119_I173_D0.5870.64
63_V87_R0.5860.64
276_Q282_L0.5830.63
98_L121_H0.5790.63
23_E26_L0.5790.63
78_Q134_Y0.5770.63
43_R250_D0.5760.63
38_E43_R0.5760.63
109_A189_V0.5720.62
210_L217_V0.5710.62
229_N244_L0.5700.62
127_V130_T0.5680.61
232_A256_V0.5670.61
116_S134_Y0.5660.61
243_F254_E0.5650.61
144_V150_V0.5630.61
160_K245_I0.5630.61
114_E187_K0.5610.60
40_V95_T0.5610.60
134_Y141_M0.5600.60
143_L149_D0.5600.60
104_M112_R0.5580.60
199_A266_A0.5580.60
109_A118_L0.5570.60
17_R22_V0.5570.60
123_G133_Q0.5560.60
22_V26_L0.5550.60
35_V94_A0.5540.60
72_S174_T0.5520.59
200_T230_R0.5520.59
92_F106_V0.5510.59
211_A240_K0.5500.59
231_L265_T0.5500.59
16_D272_D0.5490.59
121_H145_E0.5470.59
119_I169_L0.5440.58
153_L184_R0.5420.58
68_I92_F0.5400.58
276_Q283_Q0.5400.58
204_Q268_A0.5400.58
77_S141_M0.5390.57
200_T231_L0.5380.57
206_A210_L0.5350.57
246_D250_D0.5350.57
115_E161_L0.5310.56
154_T157_N0.5310.56
232_A239_G0.5310.56
255_W259_V0.5310.56
34_Y57_I0.5300.56
267_G275_V0.5280.56
26_L53_G0.5250.56
146_S173_D0.5240.55
57_I84_A0.5230.55
6_A220_V0.5220.55
106_V162_S0.5210.55
232_A245_I0.5210.55
110_S133_Q0.5210.55
5_L201_T0.5200.55
142_Y154_T0.5200.55
10_G210_L0.5200.55
46_V235_A0.5190.55
110_S141_M0.5160.54
260_K280_A0.5160.54
20_S23_E0.5150.54
113_G156_K0.5150.54
40_V98_L0.5150.54
84_A91_V0.5150.54
11_F15_V0.5140.54
169_L174_T0.5130.54
116_S193_K0.5130.54
155_V161_L0.5120.54
240_K259_V0.5110.54
255_W258_E0.5100.53
106_V110_S0.5100.53
108_R111_R0.5090.53
93_D100_T0.5080.53
31_A90_T0.5070.53
111_R156_K0.5060.53
4_L210_L0.5050.53
9_R103_H0.5020.52
174_T178_I0.5010.52
200_T268_A0.5000.52
57_I69_L0.5000.52
119_I174_T0.5000.52
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4h4dA 1 0.981 100 0.039 Contact Map
4n7bA 1 0.9905 100 0.039 Contact Map
3dnfA 2 0.8892 100 0.082 Contact Map
4toiA 2 0.731 100 0.692 Contact Map
4jenA 2 0.3481 85.4 0.956 Contact Map
3uk0A 1 0.4905 75.5 0.96 Contact Map
4f40A 1 0.8006 72.1 0.961 Contact Map
3hsyA 2 0.462 71.2 0.961 Contact Map
3k4hA 2 0.4968 68.4 0.962 Contact Map
4rk6A 2 0.4778 66.6 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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