GREMLIN Database
ISPG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
UniProt: P62620 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10370
Length: 372 (347)
Sequences: 1739 (757)
Seq/√Len: 40.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
193_R230_E4.9061.00
76_K103_D3.8571.00
144_D163_R3.5381.00
14_R26_A3.3091.00
347_Q350_A3.0281.00
155_Q194_L3.0251.00
58_I84_V2.9981.00
279_D282_G2.7871.00
14_R21_P2.6501.00
16_Y21_P2.6081.00
115_E170_R2.5931.00
96_K99_E2.5371.00
15_I177_K2.5201.00
189_V226_L2.5161.00
165_V178_V2.4071.00
166_D170_R2.3971.00
45_V79_V2.3551.00
188_A207_I2.3351.00
217_A250_V2.3321.00
52_E80_N2.3151.00
13_T174_D2.2971.00
216_G240_L2.2641.00
45_V78_Q2.2531.00
10_R196_A2.2431.00
326_K338_R2.2421.00
74_L77_Q2.2221.00
327_S344_M2.2101.00
50_A243_D2.2011.00
104_C177_K2.2001.00
36_T44_T2.1960.99
41_V45_V2.1910.99
266_N299_D2.1780.99
98_A125_A2.1460.99
98_A130_I2.1310.99
238_V254_I2.1210.99
79_V83_L2.0710.99
12_S196_A2.0580.99
291_L300_V2.0310.99
142_E160_S1.9890.99
40_D43_A1.9450.99
39_T65_T1.9330.99
142_E163_R1.9260.99
340_D343_D1.8960.98
129_N175_Q1.8780.98
32_S51_L1.8760.98
49_K80_N1.8750.98
178_V195_L1.8460.98
290_R349_E1.8200.98
178_V199_I1.8180.98
283_T345_I1.8090.98
13_T200_D1.7710.98
120_M123_D1.7710.98
52_E79_V1.7690.98
30_V252_F1.7680.98
222_I254_I1.7660.98
14_R25_G1.7580.98
73_K100_Y1.7380.97
241_A269_A1.7260.97
84_V104_C1.7020.97
30_V251_G1.7000.97
180_V195_L1.6980.97
16_Y19_N1.6750.97
169_D201_Q1.6680.97
148_K159_E1.6660.97
46_N50_A1.6480.96
278_F322_G1.6460.96
45_V80_N1.6080.96
242_A246_E1.6030.96
42_E78_Q1.6000.96
277_E324_N1.5980.96
34_T44_T1.5930.96
57_D261_R1.5790.95
50_A245_V1.5700.95
351_R354_A1.5640.95
145_L156_A1.5540.95
282_G286_A1.5530.95
167_H171_L1.5510.95
122_V171_L1.5420.95
343_D347_Q1.5240.94
122_V168_L1.5170.94
337_D347_Q1.5090.94
246_E249_K1.5060.94
325_K341_N1.4910.94
29_A58_I1.4610.93
61_V79_V1.4580.93
72_F102_V1.4430.92
38_T44_T1.4420.92
138_A182_A1.4280.92
195_L199_I1.4240.92
221_A225_G1.4210.92
290_R346_D1.4210.92
162_M199_I1.4160.92
29_A57_D1.4150.92
205_L236_L1.4100.92
76_K83_L1.4060.91
212_G284_V1.3980.91
29_A251_G1.3920.91
145_L163_R1.3620.90
66_M96_K1.3610.90
116_E120_M1.3590.90
348_L352_I1.3550.90
207_I223_G1.3520.90
226_L230_E1.3470.90
224_L238_V1.3460.89
119_R123_D1.3460.89
20_V29_A1.3370.89
26_A29_A1.3350.89
327_S339_L1.3350.89
252_F263_R1.3340.89
320_V344_M1.3290.89
119_R171_L1.3250.89
153_T156_A1.3120.88
138_A141_L1.3060.88
349_E353_R1.2960.88
54_V245_V1.2940.88
194_L198_Q1.2910.87
118_I164_H1.2820.87
214_R253_D1.2640.86
329_L339_L1.2640.86
350_A354_A1.2630.86
14_R24_D1.2630.86
329_L348_L1.2620.86
49_K52_E1.2600.86
21_P25_G1.2550.86
62_S274_S1.2380.85
99_E124_C1.2370.85
105_L125_A1.2370.85
70_E96_K1.2320.85
182_A187_L1.2310.85
226_L229_S1.2310.85
337_D351_R1.2240.84
157_L180_V1.2200.84
95_L102_V1.1970.83
343_D346_D1.1860.83
21_P26_A1.1750.82
35_N276_Q1.1570.81
222_I225_G1.1480.80
115_E119_R1.1470.80
286_A290_R1.1410.80
15_I21_P1.1400.80
84_V177_K1.1380.80
289_Q292_E1.1350.80
11_K200_D1.1350.80
134_I164_H1.1240.79
249_K292_E1.1230.79
285_N289_Q1.1060.78
98_A124_C1.1040.78
34_T38_T1.1000.77
49_K78_Q1.0910.77
242_A281_I1.0900.77
41_V75_I1.0870.77
190_E193_R1.0840.76
126_R172_N1.0810.76
39_T68_A1.0790.76
168_L173_F1.0790.76
319_G330_Y1.0720.76
301_S316_S1.0710.76
41_V74_L1.0700.75
283_T286_A1.0660.75
328_G338_R1.0660.75
95_L128_K1.0610.75
44_T47_Q1.0570.75
222_I226_L1.0540.74
213_A242_A1.0530.74
205_L227_L1.0520.74
146_Q314_L1.0440.74
155_Q198_Q1.0440.74
318_L351_R1.0430.74
331_E336_K1.0330.73
176_F201_Q1.0240.72
212_G288_E1.0220.72
95_L124_C1.0190.72
52_E81_V1.0120.71
21_P29_A1.0090.71
117_R120_M1.0070.71
124_C127_D1.0060.71
69_A100_Y0.9950.70
252_F261_R0.9920.70
328_G344_M0.9790.69
286_A345_I0.9750.68
17_V29_A0.9750.68
286_A289_Q0.9670.68
45_V76_K0.9660.68
25_G318_L0.9660.68
164_H168_L0.9650.68
97_V100_Y0.9610.67
321_T330_Y0.9610.67
76_K79_V0.9600.67
41_V71_A0.9590.67
222_I257_S0.9590.67
124_C128_K0.9580.67
96_K100_Y0.9530.67
11_K25_G0.9500.66
107_I113_G0.9460.66
42_E46_N0.9380.65
45_V49_K0.9340.65
267_F291_L0.9320.65
72_F76_K0.9280.64
165_V180_V0.9230.64
157_L187_L0.9170.64
157_L182_A0.9150.63
155_Q190_E0.9140.63
56_A81_V0.9110.63
221_A254_I0.9060.63
146_Q156_A0.9040.62
22_I29_A0.8990.62
168_L176_F0.8990.62
65_T68_A0.8980.62
64_P68_A0.8930.61
159_E163_R0.8870.61
188_A224_L0.8860.61
13_T175_Q0.8850.61
216_G242_A0.8820.61
291_L294_I0.8820.61
123_D127_D0.8780.60
166_D169_D0.8750.60
219_K266_N0.8710.60
184_D266_N0.8690.59
236_L251_G0.8660.59
284_V287_L0.8660.59
214_R217_A0.8650.59
35_N47_Q0.8630.59
38_T71_A0.8610.59
306_V328_G0.8600.59
331_E351_R0.8590.58
339_L347_Q0.8570.58
27_P261_R0.8560.58
214_R249_K0.8530.58
287_L302_I0.8500.58
157_L165_V0.8490.58
34_T59_V0.8440.57
326_K340_D0.8430.57
263_R295_I0.8420.57
45_V52_E0.8420.57
10_R200_D0.8410.57
309_G319_G0.8360.56
44_T61_V0.8350.56
90_D93_I0.8350.56
163_R166_D0.8330.56
162_M198_Q0.8310.56
169_D174_D0.8300.56
146_Q152_P0.8300.56
189_V229_S0.8280.56
229_S258_L0.8270.56
57_D252_F0.8270.56
38_T47_Q0.8250.55
49_K79_V0.8240.55
165_V199_I0.8240.55
207_I227_L0.8190.55
28_I236_L0.8170.55
17_V104_C0.8130.54
115_E167_H0.8100.54
184_D187_L0.8100.54
272_T276_Q0.8100.54
83_L103_D0.8090.54
37_R68_A0.8090.54
207_I257_S0.8070.54
241_A280_V0.8060.54
284_V302_I0.8050.54
145_L148_K0.8040.53
194_L197_K0.8030.53
62_S93_I0.8010.53
337_D350_A0.7980.53
66_M70_E0.7970.53
214_R218_V0.7960.53
35_N244_P0.7950.53
166_D201_Q0.7920.52
36_T47_Q0.7900.52
112_I118_I0.7850.52
293_D353_R0.7800.51
301_S310_P0.7780.51
242_A285_N0.7770.51
303_I316_S0.7750.51
79_V103_D0.7720.51
180_V191_S0.7720.51
20_V58_I0.7720.51
25_G234_D0.7700.50
213_A246_E0.7670.50
72_F97_V0.7630.50
207_I226_L0.7620.50
164_H167_H0.7610.49
10_R25_G0.7560.49
59_V79_V0.7560.49
220_S238_V0.7540.49
68_A274_S0.7530.49
265_I291_L0.7530.49
189_V207_I0.7510.49
49_K53_R0.7490.48
158_L194_L0.7480.48
303_I310_P0.7470.48
17_V20_V0.7430.48
22_I177_K0.7410.48
34_T48_I0.7410.48
318_L352_I0.7400.48
105_L199_I0.7360.47
142_E164_H0.7310.47
99_E128_K0.7310.47
149_Y153_T0.7300.47
349_E352_I0.7290.47
287_L300_V0.7250.46
16_Y20_V0.7200.46
15_I104_C0.7150.45
267_F302_I0.7150.45
34_T47_Q0.7150.45
88_H274_S0.7130.45
141_L154_P0.7120.45
39_T277_E0.7100.45
213_A218_V0.7070.45
115_E171_L0.7060.44
14_R29_A0.7050.44
300_V318_L0.7020.44
193_R226_L0.7020.44
95_L99_E0.7010.44
263_R297_P0.7010.44
195_L263_R0.7000.44
287_L345_I0.7000.44
258_L261_R0.6980.44
22_I104_C0.6980.44
193_R229_S0.6980.44
278_F283_T0.6970.44
37_R277_E0.6970.44
212_G302_I0.6940.43
38_T75_I0.6930.43
75_I81_V0.6920.43
154_P187_L0.6910.43
162_M178_V0.6890.43
215_S253_D0.6890.43
221_A224_L0.6860.43
91_Y95_L0.6850.43
276_Q303_I0.6820.42
217_A221_A0.6800.42
121_V124_C0.6790.42
48_I71_A0.6790.42
280_V341_N0.6780.42
56_A248_I0.6770.42
57_D79_V0.6760.42
294_I297_P0.6750.42
80_N83_L0.6710.41
341_N348_L0.6710.41
45_V103_D0.6680.41
143_K332_D0.6660.41
317_T332_D0.6650.41
142_E183_S0.6640.41
320_V328_G0.6610.40
331_E347_Q0.6610.40
63_V69_A0.6570.40
306_V335_R0.6560.40
14_R20_V0.6520.40
346_D349_E0.6510.39
29_A104_C0.6500.39
122_V167_H0.6480.39
145_L159_E0.6460.39
267_F284_V0.6460.39
73_K77_Q0.6430.39
329_L347_Q0.6430.39
336_K354_A0.6420.39
57_D263_R0.6410.39
253_D256_K0.6400.39
32_S56_A0.6340.38
89_F306_V0.6340.38
335_R338_R0.6330.38
71_A100_Y0.6320.38
196_A203_L0.6320.38
69_A96_K0.6310.38
327_S341_N0.6300.38
329_L345_I0.6270.37
44_T48_I0.6250.37
50_A53_R0.6250.37
154_P194_L0.6240.37
8_Q11_K0.6220.37
343_D350_A0.6190.37
35_N281_I0.6150.36
64_P88_H0.6120.36
184_D188_A0.6100.36
45_V83_L0.6070.36
197_K230_E0.6040.35
160_S183_S0.6040.35
91_Y121_V0.6030.35
16_Y129_N0.6020.35
48_I75_I0.6000.35
222_I238_V0.6000.35
22_I83_L0.5990.35
313_A321_T0.5980.35
182_A188_A0.5980.35
72_F79_V0.5970.35
340_D344_M0.5970.35
251_G350_A0.5960.35
152_P182_A0.5960.35
38_T48_I0.5950.35
76_K80_N0.5950.35
156_A159_E0.5950.35
310_P313_A0.5940.35
45_V75_I0.5930.34
30_V227_L0.5930.34
168_L192_Y0.5920.34
182_A186_F0.5900.34
93_I241_A0.5880.34
73_K96_K0.5880.34
290_R353_R0.5880.34
289_Q334_V0.5860.34
296_T352_I0.5860.34
182_A207_I0.5840.34
287_L344_M0.5840.34
152_P156_A0.5830.34
192_Y203_L0.5830.34
329_L340_D0.5820.34
329_L337_D0.5820.34
113_G118_I0.5810.33
276_Q301_S0.5780.33
76_K119_R0.5780.33
114_N120_M0.5770.33
46_N53_R0.5760.33
153_T187_L0.5760.33
39_T64_P0.5750.33
289_Q346_D0.5740.33
45_V81_V0.5740.33
134_I192_Y0.5740.33
224_L236_L0.5730.33
152_P315_V0.5720.33
222_I229_S0.5700.33
163_R167_H0.5690.33
122_V178_V0.5680.32
91_Y113_G0.5680.32
262_S297_P0.5660.32
123_D126_R0.5650.32
232_I299_D0.5650.32
253_D267_F0.5640.32
44_T75_I0.5640.32
180_V188_A0.5620.32
28_I57_D0.5590.32
177_K252_F0.5550.31
183_S219_K0.5550.31
36_T75_I0.5530.31
259_R267_F0.5510.31
70_E346_D0.5510.31
122_V126_R0.5510.31
152_P159_E0.5500.31
272_T321_T0.5490.31
122_V299_D0.5490.31
92_R117_R0.5490.31
99_E127_D0.5480.31
54_V249_K0.5460.31
90_D243_D0.5450.31
214_R250_V0.5450.31
61_V72_F0.5450.31
177_K263_R0.5450.31
186_F229_S0.5450.31
22_I58_I0.5440.31
131_P176_F0.5440.31
180_V192_Y0.5430.30
98_A128_K0.5430.30
142_E145_L0.5420.30
104_C175_Q0.5420.30
283_T341_N0.5400.30
189_V230_E0.5400.30
9_R12_S0.5390.30
113_G121_V0.5390.30
131_P175_Q0.5380.30
298_M349_E0.5380.30
36_T43_A0.5380.30
113_G143_K0.5350.30
47_Q244_P0.5330.30
238_V269_A0.5330.30
22_I26_A0.5330.30
63_V102_V0.5330.30
36_T40_D0.5330.30
331_E334_V0.5320.30
195_L203_L0.5320.30
318_L331_E0.5320.30
83_L147_E0.5320.30
17_V175_Q0.5320.30
223_G238_V0.5310.30
62_S88_H0.5300.29
112_I143_K0.5300.29
184_D299_D0.5300.29
243_D246_E0.5290.29
276_Q281_I0.5290.29
241_A303_I0.5270.29
167_H170_R0.5270.29
40_D47_Q0.5270.29
59_V81_V0.5270.29
205_L224_L0.5270.29
264_G344_M0.5260.29
276_Q283_T0.5250.29
138_A191_S0.5250.29
306_V326_K0.5240.29
34_T125_A0.5230.29
146_Q151_E0.5230.29
320_V329_L0.5220.29
40_D44_T0.5210.29
41_V61_V0.5210.29
294_I353_R0.5190.29
272_T278_F0.5190.29
276_Q280_V0.5170.28
196_A232_I0.5170.28
52_E103_D0.5170.28
238_V251_G0.5140.28
25_G42_E0.5110.28
93_I269_A0.5110.28
203_L232_I0.5100.28
149_Y159_E0.5090.28
134_I168_L0.5090.28
9_R234_D0.5080.28
173_F176_F0.5070.28
89_F114_N0.5070.28
114_N117_R0.5070.28
298_M317_T0.5060.28
104_C131_P0.5060.28
191_S205_L0.5050.28
32_S243_D0.5050.28
154_P159_E0.5040.27
197_K354_A0.5040.27
72_F201_Q0.5030.27
325_K339_L0.5030.27
309_G322_G0.5020.27
57_D81_V0.5010.27
11_K119_R0.5010.27
28_I56_A0.5010.27
323_G341_N0.5010.27
120_M124_C0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4s39A 2 0.9543 100 0.253 Contact Map
3noyA 2 0.9435 100 0.295 Contact Map
4mwaA 4 0.7016 100 0.502 Contact Map
2yr1A 2 0.6022 100 0.739 Contact Map
4g38A 1 0.914 99.8 0.871 Contact Map
1zj8A 1 0.9624 99.7 0.887 Contact Map
4nirA 3 0.6317 98.9 0.927 Contact Map
4djdD 1 0.6344 98.6 0.934 Contact Map
2yciX 2 0.6398 98.5 0.935 Contact Map
1eyeA 2 0.6075 98.4 0.937 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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