GREMLIN Database
MDH - Malate dehydrogenase
UniProt: P61889 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG10576
Length: 312 (289)
Sequences: 4882 (2849)
Seq/√Len: 167.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_A70_A4.4331.00
72_V235_G3.1601.00
204_K208_N3.0421.00
289_Q293_E2.9781.00
74_L235_G2.7501.00
95_A127_I2.5951.00
117_I231_A2.5741.00
79_V93_V2.5061.00
4_A67_L2.4801.00
23_Q236_L2.4041.00
67_L109_C2.3981.00
102_V128_A2.3961.00
246_Q271_G2.3041.00
104_Q107_K2.2981.00
297_D301_K2.2821.00
140_K255_E2.2351.00
271_G276_E2.2121.00
264_F292_L2.1921.00
205_R212_E2.1851.00
246_Q276_E2.1321.00
201_D205_R2.0671.00
269_L277_E2.0271.00
132_L263_F2.0081.00
106_A138_Y2.0061.00
268_L275_V2.0001.00
100_N104_Q1.9681.00
174_I185_L1.9681.00
161_L198_E1.9261.00
113_C238_L1.8941.00
259_Q293_E1.8881.00
290_N294_G1.8621.00
102_V135_A1.8601.00
171_V189_V1.8581.00
264_F296_L1.8461.00
99_K103_Q1.8341.00
293_E297_D1.8231.00
91_F302_D1.8071.00
138_Y142_K1.7701.00
2_K29_E1.7401.00
259_Q292_L1.7201.00
98_V128_A1.7121.00
103_Q134_K1.7101.00
146_V231_A1.6731.00
103_Q107_K1.6561.00
286_A289_Q1.6561.00
39_T43_A1.6491.00
2_K31_S1.6431.00
85_M93_V1.5741.00
5_V17_A1.5671.00
183_L288_E1.5651.00
126_A303_I1.5641.00
291_A294_G1.5631.00
96_G99_K1.5601.00
147_T265_S1.5481.00
173_V184_P1.5461.00
253_Y263_F1.5431.00
47_S160_E1.5411.00
254_V266_Q1.5391.00
200_A204_K1.5361.00
3_V20_L1.5301.00
16_L231_A1.5151.00
286_A290_N1.5001.00
273_N276_E1.4991.00
125_V263_F1.4951.00
81_R210_G1.4771.00
64_T108_T1.4621.00
114_I128_A1.4561.00
64_T107_K1.4521.00
65_P68_E1.4431.00
148_T230_A1.4391.00
47_S156_T1.4371.00
159_A169_V1.4261.00
294_G297_D1.4151.00
259_Q296_L1.4051.00
158_V169_V1.3731.00
184_P203_T1.3671.00
41_G216_A1.3401.00
49_I229_Q1.3391.00
67_L70_A1.3251.00
18_L228_G1.3201.00
287_F291_A1.3181.00
300_K304_A1.3171.00
208_N211_T1.3161.00
116_I128_A1.3141.00
181_T298_T1.3051.00
75_I116_I1.3021.00
126_A133_K1.2921.00
105_V138_Y1.2851.00
204_K207_Q1.2781.00
99_K134_K1.2741.00
198_E201_D1.2731.00
148_T249_V1.2631.00
212_E216_A1.2621.00
290_N293_E1.2551.00
244_G272_K1.2531.00
165_Q168_E1.2531.00
86_D89_D1.2521.00
161_L201_D1.2521.00
301_K305_L1.2491.00
71_D110_P1.2411.00
105_V114_I1.2331.00
106_A136_G1.2331.00
60_G66_A1.2291.00
187_S285_S1.2281.00
98_V102_V1.2261.00
212_E215_E1.2191.00
113_C142_K1.2191.00
72_V115_G1.2181.00
259_Q300_K1.2081.00
73_V105_V1.2071.00
16_L235_G1.1931.00
20_L30_L1.1881.00
121_V145_G1.1851.00
18_L22_T1.1771.00
37_P63_A1.1721.00
255_E260_Y1.1691.00
16_L74_L1.1681.00
79_V82_K1.1651.00
3_V27_G1.1631.00
31_S66_A1.1611.00
187_S288_E1.1531.00
168_E190_P1.1461.00
100_N103_Q1.1461.00
144_F238_L1.1461.00
109_C112_A1.1431.00
2_K69_G1.1361.00
254_V257_D1.1341.00
233_R247_G1.1321.00
294_G298_T1.1311.00
119_N177_H1.1261.00
93_V96_G1.1261.00
90_L94_N1.1231.00
111_K139_D1.1231.00
248_V269_L1.1021.00
48_H209_A1.1011.00
293_E296_L1.0881.00
73_V112_A1.0851.00
11_G224_T1.0831.00
135_A138_Y1.0781.00
127_I134_K1.0671.00
87_R119_N1.0671.00
141_N273_N1.0600.99
181_T295_M1.0600.99
75_I128_A1.0590.99
236_L243_Q1.0520.99
87_R177_H1.0500.99
63_A66_A1.0460.99
14_Q42_V1.0350.99
132_L253_Y1.0340.99
121_V125_V1.0280.99
269_L276_E1.0270.99
92_N96_G1.0250.99
292_L296_L1.0210.99
16_L228_G1.0200.99
98_V124_T1.0040.99
159_A166_P1.0000.99
301_K304_A0.9910.99
132_L140_K0.9910.99
269_L279_K0.9850.99
89_D92_N0.9840.99
208_N215_E0.9800.99
167_G245_E0.9660.99
81_R214_V0.9650.99
102_V114_I0.9650.99
79_V97_I0.9650.99
70_A73_V0.9640.99
71_D111_K0.9610.99
155_N170_E0.9570.99
33_Y64_T0.9550.99
185_L284_L0.9530.99
259_Q289_Q0.9530.99
31_S70_A0.9530.99
287_F290_N0.9520.99
72_V239_V0.9480.99
125_V145_G0.9460.99
196_E283_T0.9450.99
228_G232_A0.9440.99
38_V42_V0.9430.99
156_T160_E0.9400.99
23_Q232_A0.9330.99
257_D279_K0.9280.99
115_G234_F0.9240.99
181_T294_G0.9230.99
14_Q228_G0.9210.99
68_E108_T0.9200.99
17_A32_L0.9200.99
139_D142_K0.9200.99
29_E58_F0.9180.98
284_L292_L0.9130.98
85_M89_D0.9120.98
106_A135_A0.9080.98
217_K221_G0.9030.98
65_P108_T0.9010.98
210_G214_V0.8980.98
20_L26_S0.8980.98
69_G110_P0.8970.98
297_D304_A0.8960.98
197_Q201_D0.8930.98
154_S171_V0.8910.98
143_L263_F0.8900.98
73_V109_C0.8880.98
164_K169_V0.8820.98
75_I98_V0.8820.98
57_G60_G0.8800.98
151_I155_N0.8800.98
22_T25_P0.8770.98
62_D66_A0.8760.98
123_T302_D0.8760.98
74_L115_G0.8710.98
157_F205_R0.8710.98
56_K165_Q0.8670.98
132_L143_L0.8560.98
251_C268_L0.8540.98
27_G30_L0.8440.97
157_F160_E0.8410.97
138_Y143_L0.8400.97
231_A235_G0.8350.97
64_T101_L0.8350.97
83_P215_E0.8250.97
145_G251_C0.8190.97
81_R90_L0.8180.97
115_G238_L0.8170.97
19_L228_G0.8140.97
11_G14_Q0.8140.97
148_T234_F0.8130.97
162_K169_V0.8060.97
16_L20_L0.8050.97
26_S232_A0.8040.97
112_A138_Y0.8030.96
266_Q278_R0.8020.96
234_F251_C0.8010.96
81_R85_M0.7980.96
255_E279_K0.7970.96
115_G235_G0.7960.96
90_L214_V0.7950.96
121_V147_T0.7930.96
203_T207_Q0.7880.96
40_P216_A0.7880.96
180_V211_T0.7860.96
256_G280_S0.7750.96
6_L101_L0.7690.96
271_G274_G0.7680.95
88_S123_T0.7670.95
81_R211_T0.7650.95
211_T215_E0.7640.95
16_L76_S0.7640.95
298_T302_D0.7610.95
5_V16_L0.7580.95
158_V162_K0.7540.95
246_Q269_L0.7520.95
98_V116_I0.7520.95
297_D300_K0.7510.95
75_I101_L0.7490.95
195_T199_V0.7480.95
102_V106_A0.7470.95
140_K253_Y0.7460.95
276_E279_K0.7450.95
302_D305_L0.7430.95
173_V203_T0.7430.95
7_G10_G0.7390.94
174_I183_L0.7370.94
126_A132_L0.7360.94
213_V226_S0.7360.94
11_G222_S0.7350.94
97_I118_T0.7340.94
154_S184_P0.7340.94
19_L22_T0.7310.94
77_A97_I0.7310.94
15_A225_L0.7300.94
257_D277_E0.7300.94
193_S196_E0.7280.94
37_P40_P0.7280.94
217_K223_A0.7250.94
126_A261_A0.7210.94
201_D204_K0.7190.94
113_C144_F0.7180.94
236_L245_E0.7170.94
244_G271_G0.7170.94
33_Y67_L0.7170.94
165_Q247_G0.7160.93
92_N305_L0.7160.93
183_L295_M0.7120.93
186_L206_I0.7070.93
36_A39_T0.7050.93
69_G111_K0.7040.93
87_R90_L0.7020.93
20_L232_A0.7020.93
255_E261_A0.7010.93
188_Q281_I0.6990.93
15_A224_T0.6990.93
257_D266_Q0.6960.92
82_K93_V0.6940.92
42_V228_G0.6910.92
208_N212_E0.6910.92
74_L231_A0.6900.92
214_V222_S0.6890.92
258_G266_Q0.6870.92
106_A112_A0.6850.92
88_S302_D0.6830.92
15_A19_L0.6760.91
20_L235_G0.6750.91
20_L27_G0.6750.91
154_S189_V0.6740.91
58_F61_E0.6740.91
37_P61_E0.6720.91
141_N274_G0.6710.91
4_A31_S0.6700.91
117_I146_V0.6700.91
250_E270_L0.6690.91
192_V196_E0.6680.91
226_S229_Q0.6670.91
155_N233_R0.6660.91
96_G100_N0.6640.91
17_A46_L0.6630.91
70_A109_C0.6620.91
158_V171_V0.6580.90
106_A114_I0.6570.90
280_S283_T0.6570.90
210_G223_A0.6560.90
200_A287_F0.6550.90
48_H156_T0.6520.90
64_T68_E0.6500.90
33_Y101_L0.6320.88
88_S298_T0.6290.88
87_R150_D0.6260.88
159_A164_K0.6260.88
42_V217_K0.6230.88
111_K142_K0.6210.88
23_Q26_S0.6200.87
64_T104_Q0.6190.87
4_A109_C0.6180.87
213_V217_K0.6160.87
128_A143_L0.6150.87
296_L300_K0.6140.87
256_G259_Q0.6140.87
58_F62_D0.6130.87
105_V108_T0.6100.87
133_K253_Y0.6080.86
19_L232_A0.6070.86
237_S250_E0.6060.86
86_D180_V0.6040.86
88_S305_L0.6040.86
104_Q108_T0.6030.86
274_G279_K0.6020.86
142_K273_N0.6010.86
102_V134_K0.5990.86
183_L284_L0.5990.86
185_L188_Q0.5990.86
160_E205_R0.5980.85
121_V146_V0.5980.85
5_V30_L0.5970.85
75_I102_V0.5970.85
150_D153_R0.5950.85
183_L291_A0.5940.85
21_K46_L0.5940.85
78_G118_T0.5910.85
59_S62_D0.5900.85
277_E280_S0.5900.85
43_A47_S0.5880.85
14_Q225_L0.5870.84
284_L293_E0.5870.84
210_G222_S0.5870.84
186_L189_V0.5870.84
275_V278_R0.5860.84
78_G81_R0.5840.84
262_R300_K0.5830.84
67_L106_A0.5830.84
18_L225_L0.5820.84
21_K30_L0.5810.84
128_A135_A0.5790.84
95_A134_K0.5790.84
266_Q277_E0.5770.83
76_S117_I0.5750.83
271_G275_V0.5750.83
31_S60_G0.5740.83
203_T291_A0.5730.83
177_H210_G0.5690.83
136_G143_L0.5670.82
31_S62_D0.5670.82
98_V134_K0.5640.82
281_I284_L0.5610.82
53_V229_Q0.5590.81
162_K194_F0.5580.81
15_A18_L0.5540.81
234_F249_V0.5540.81
285_S289_Q0.5530.81
140_K263_F0.5510.81
274_G277_E0.5500.80
132_L135_A0.5490.80
256_G278_R0.5420.79
13_G17_A0.5420.79
172_P190_P0.5410.79
262_R296_L0.5410.79
183_L203_T0.5410.79
176_G179_G0.5410.79
51_T152_I0.5390.79
6_L67_L0.5390.79
233_R245_E0.5380.79
94_N124_T0.5380.79
133_K136_G0.5360.79
68_E107_K0.5340.78
72_V238_L0.5330.78
69_G109_C0.5310.78
31_S58_F0.5300.78
101_L105_V0.5300.78
266_Q280_S0.5290.78
283_T289_Q0.5290.78
132_L138_Y0.5260.77
272_K276_E0.5240.77
237_S245_E0.5240.77
158_V194_F0.5230.77
249_V265_S0.5230.77
154_S183_L0.5220.77
174_I281_I0.5210.77
300_K303_I0.5210.77
223_A227_M0.5210.77
200_A286_A0.5190.77
284_L288_E0.5180.76
5_V20_L0.5140.76
36_A61_E0.5140.76
42_V225_L0.5140.76
214_V223_A0.5130.76
95_A99_K0.5130.76
257_D280_S0.5130.76
157_F161_L0.5120.76
144_F234_F0.5110.75
174_I284_L0.5100.75
152_I226_S0.5100.75
99_K127_I0.5060.75
3_V30_L0.5040.74
85_M90_L0.5040.74
155_N171_V0.5020.74
256_G260_Y0.5010.74
17_A30_L0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3hhpA 2 1 100 0.209 Contact Map
1mldA 3 0.9904 100 0.282 Contact Map
3nepX 4 0.9327 100 0.287 Contact Map
2d4aB 4 0.9455 100 0.297 Contact Map
1guzA 4 0.9423 100 0.304 Contact Map
4cl3A 4 0.9487 100 0.306 Contact Map
4plzA 4 0.9135 100 0.313 Contact Map
2v6bA 4 0.8558 100 0.315 Contact Map
3p7mA 4 0.9519 100 0.316 Contact Map
1hyhA 4 0.9231 100 0.316 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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