GREMLIN Database
PTHB - Glucitol/sorbitol-specific phosphotransferase enzyme IIB component
UniProt: P56580 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14373
Length: 319 (314)
Sequences: 208 (107)
Seq/√Len: 6.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
27_I42_L3.9380.93
19_L65_G3.9330.93
3_H20_K3.4330.87
29_Y36_P3.1330.81
60_A83_R3.1020.81
94_G108_Y3.0960.80
86_P107_I3.0340.79
171_D175_K2.8070.73
247_Q250_L2.7480.71
84_R106_D2.7200.71
42_L66_V2.6900.70
6_I19_L2.6680.69
84_R107_I2.6640.69
26_K63_E2.6250.68
75_L281_A2.5240.65
95_K108_Y2.5080.64
85_I107_I2.3930.60
84_R104_V2.3870.60
157_S219_L2.3580.59
6_I88_I2.2970.57
28_V64_I2.2930.57
159_V218_A2.2720.56
59_P85_I2.2710.56
170_I178_L2.2060.54
34_T52_D2.1620.52
312_F315_G2.1360.51
159_V240_I1.9880.46
274_I294_S1.9270.44
222_S300_F1.9230.44
41_K175_K1.9160.43
83_R279_S1.9080.43
77_C89_N1.9060.43
246_V249_G1.8710.42
70_D235_V1.8560.41
108_Y193_A1.8540.41
28_V67_A1.8390.41
40_D44_Q1.8310.40
66_V69_I1.8210.40
188_I244_I1.8030.39
29_Y35_R1.7280.37
6_I96_S1.7170.37
104_V107_I1.7100.36
5_R18_E1.7070.36
89_N108_Y1.7000.36
222_S266_N1.6710.35
51_I88_I1.6370.34
56_E289_R1.6340.34
149_A160_A1.6280.34
113_K116_N1.6280.34
11_G111_G1.6080.33
205_A217_L1.5960.33
57_G60_A1.5910.32
261_A303_G1.5650.32
166_G170_I1.5460.31
194_S252_N1.5310.31
5_R286_D1.5300.31
136_T288_V1.5230.30
219_L240_I1.5190.30
4_I22_T1.5030.30
75_L79_I1.4950.30
103_I185_S1.4930.29
30_I52_D1.4760.29
75_L294_S1.4630.29
6_I66_V1.4530.28
6_I309_I1.4510.28
176_T279_S1.4420.28
86_P106_D1.4320.28
28_V53_G1.4320.28
164_Q188_I1.4160.27
27_I66_V1.4120.27
183_F261_A1.4110.27
7_E118_T1.4080.27
202_H205_A1.4070.27
308_L312_F1.4060.27
95_K104_V1.3690.26
41_K44_Q1.3570.26
39_V294_S1.3470.25
191_I244_I1.3430.25
13_W52_D1.3380.25
46_T50_A1.3340.25
249_G266_N1.3290.25
45_L90_I1.3280.25
45_L119_V1.3270.25
86_P204_L1.3270.25
197_G261_A1.3190.25
42_L88_I1.3130.24
82_K152_G1.3090.24
269_A311_W1.3080.24
64_I85_I1.3070.24
223_F269_A1.3030.24
94_G193_A1.3010.24
34_T75_L1.2980.24
171_D199_W1.2950.24
95_K193_A1.2950.24
183_F197_G1.2930.24
35_R39_V1.2880.24
35_R40_D1.2850.24
102_Y147_L1.2840.24
90_I146_G1.2810.24
155_M242_V1.2670.23
210_H290_V1.2630.23
207_L220_I1.2540.23
67_A257_L1.2540.23
83_R108_Y1.2530.23
269_A306_T1.2520.23
41_K114_E1.2470.23
52_D117_I1.2410.23
15_G191_I1.2350.22
156_G167_R1.2310.22
38_I117_I1.2170.22
55_K68_V1.2080.22
30_I281_A1.2060.22
15_G89_N1.1960.22
228_P296_L1.1920.21
16_P94_G1.1880.21
151_V226_L1.1850.21
222_S269_A1.1790.21
137_S141_T1.1740.21
184_V208_A1.1730.21
197_G235_V1.1660.21
192_M216_M1.1650.21
203_G217_L1.1630.21
108_Y279_S1.1570.21
89_N94_G1.1510.20
288_V311_W1.1470.20
16_P89_N1.1440.20
150_K170_I1.1420.20
67_A214_L1.1420.20
84_R237_A1.1390.20
220_I242_V1.1300.20
77_C221_C1.1250.20
246_V266_N1.1130.20
13_W82_K1.1120.20
197_G303_G1.1110.20
192_M217_L1.1060.19
223_F229_F1.1050.19
104_V217_L1.1050.19
13_W50_A1.1030.19
218_A267_A1.1010.19
39_V305_P1.0900.19
16_P77_C1.0900.19
166_G281_A1.0820.19
247_Q284_R1.0700.19
4_I48_W1.0690.19
214_L267_A1.0590.18
169_T198_D1.0570.18
38_I68_V1.0560.18
42_L117_I1.0550.18
212_L215_V1.0530.18
89_N152_G1.0520.18
13_W75_L1.0490.18
175_K218_A1.0380.18
30_I67_A1.0300.18
66_V209_S1.0300.18
70_D253_I1.0230.18
139_K257_L1.0220.18
183_F191_I1.0220.18
155_M192_M1.0200.18
167_R208_A1.0190.18
13_W140_I1.0180.18
269_A302_T1.0170.18
27_I157_S1.0130.17
111_G176_T1.0130.17
16_P172_T1.0110.17
191_I235_V1.0110.17
27_I48_W1.0100.17
304_A317_I1.0060.17
215_V308_L1.0020.17
144_S315_G0.9970.17
196_L271_C0.9970.17
202_H271_C0.9940.17
60_A108_Y0.9940.17
163_F209_S0.9920.17
35_R305_P0.9900.17
267_A302_T0.9880.17
79_I281_A0.9870.17
169_T173_V0.9860.17
16_P191_I0.9810.17
228_P293_P0.9790.17
35_R304_A0.9770.17
6_I42_L0.9760.17
229_F306_T0.9710.17
27_I65_G0.9570.16
253_I303_G0.9560.16
158_T162_L0.9550.16
202_H217_L0.9540.16
183_F303_G0.9530.16
196_L266_N0.9500.16
59_P83_R0.9490.16
204_L240_I0.9480.16
84_R113_K0.9430.16
66_V160_A0.9420.16
161_V190_I0.9400.16
274_I285_Q0.9370.16
111_G171_D0.9300.16
41_K92_S0.9300.16
138_K288_V0.9290.16
209_S250_L0.9290.16
187_L300_F0.9290.16
200_I305_P0.9270.16
58_E166_G0.9230.16
234_A253_I0.9200.16
93_T160_A0.9200.16
7_E116_N0.9160.16
77_C152_G0.9130.15
52_D301_L0.9090.15
151_V155_M0.9080.15
189_G195_G0.9040.15
223_F306_T0.8990.15
13_W130_V0.8980.15
60_A169_T0.8970.15
274_I288_V0.8970.15
30_I107_I0.8890.15
210_H214_L0.8860.15
44_Q84_R0.8860.15
83_R186_A0.8830.15
15_G94_G0.8780.15
196_L247_Q0.8730.15
169_T186_A0.8710.15
271_C274_I0.8700.15
29_Y34_T0.8660.15
246_V289_R0.8650.15
196_L312_F0.8650.15
36_P94_G0.8640.15
38_I157_S0.8600.15
204_L217_L0.8570.14
67_A219_L0.8530.14
293_P296_L0.8520.14
271_C288_V0.8510.14
241_G265_I0.8500.14
29_Y37_A0.8490.14
152_G317_I0.8430.14
69_I188_I0.8410.14
235_V261_A0.8390.14
19_L215_V0.8390.14
18_E217_L0.8390.14
37_A114_E0.8380.14
210_H316_F0.8370.14
100_A146_G0.8260.14
183_F235_V0.8250.14
60_A279_S0.8250.14
4_I178_L0.8250.14
162_L185_S0.8190.14
23_P222_S0.8150.14
53_G238_Q0.8140.14
8_K111_G0.8120.14
43_A50_A0.8110.14
235_V303_G0.8100.14
4_I42_L0.8090.14
15_G77_C0.8070.14
84_R317_I0.8040.14
253_I261_A0.8030.14
19_L217_L0.8020.14
270_A294_S0.8010.13
36_P95_K0.7970.13
183_F244_I0.7950.13
155_M229_F0.7940.13
33_G189_G0.7920.13
45_L271_C0.7820.13
37_A65_G0.7800.13
183_F253_I0.7780.13
298_S301_L0.7760.13
83_R297_V0.7740.13
13_W34_T0.7700.13
134_Y138_K0.7670.13
16_P152_G0.7660.13
58_E104_V0.7640.13
226_L282_E0.7640.13
163_F168_D0.7630.13
126_Q174_L0.7620.13
88_I310_A0.7610.13
186_A235_V0.7550.13
207_L217_L0.7520.13
49_Q68_V0.7490.13
134_Y288_V0.7470.13
67_A192_M0.7450.13
8_K122_D0.7420.13
308_L315_G0.7410.13
126_Q162_L0.7390.12
90_I218_A0.7390.12
37_A305_P0.7360.12
6_I57_G0.7340.12
186_A297_V0.7330.12
163_F261_A0.7280.12
70_D165_S0.7280.12
222_S297_V0.7260.12
214_L226_L0.7240.12
312_F316_F0.7230.12
18_E174_L0.7230.12
83_R169_T0.7230.12
82_K169_T0.7200.12
167_R282_E0.7180.12
18_E133_D0.7170.12
297_V300_F0.7150.12
157_S169_T0.7120.12
191_I261_A0.7090.12
29_Y300_F0.7090.12
163_F183_F0.7080.12
244_I261_A0.7070.12
148_L158_T0.7050.12
21_A317_I0.7010.12
30_I148_L0.7010.12
83_R94_G0.6960.12
235_V253_I0.6950.12
184_V255_P0.6950.12
7_E11_G0.6920.12
191_I303_G0.6910.12
198_D239_V0.6900.12
174_L284_R0.6890.12
217_L220_I0.6880.12
104_V117_I0.6880.12
60_A64_I0.6880.12
127_P187_L0.6870.12
302_T306_T0.6830.12
143_Q149_A0.6800.12
170_I256_H0.6780.12
223_F240_I0.6760.12
21_A174_L0.6730.11
39_V43_A0.6730.11
4_I20_K0.6690.11
4_I51_I0.6670.11
111_G173_V0.6650.11
79_I294_S0.6640.11
16_P289_R0.6620.11
35_R38_I0.6590.11
29_Y304_A0.6590.11
177_I222_S0.6580.11
93_T165_S0.6570.11
10_T165_S0.6540.11
35_R269_A0.6530.11
94_G194_S0.6510.11
218_A304_A0.6500.11
33_G318_Y0.6480.11
16_P108_Y0.6460.11
214_L257_L0.6460.11
63_E115_E0.6440.11
65_G173_V0.6410.11
29_Y39_V0.6410.11
225_L240_I0.6380.11
48_W65_G0.6380.11
117_I148_L0.6380.11
27_I158_T0.6340.11
279_S311_W0.6330.11
77_C108_Y0.6330.11
48_W163_F0.6320.11
70_D292_V0.6310.11
289_R297_V0.6310.11
185_S265_I0.6280.11
88_I116_N0.6270.11
178_L237_A0.6270.11
89_N193_A0.6270.11
19_L158_T0.6260.11
90_I199_W0.6260.11
166_G186_A0.6250.11
302_T309_I0.6250.11
297_V304_A0.6240.11
20_K209_S0.6240.11
168_D203_G0.6210.11
21_A196_L0.6200.11
5_R120_V0.6180.11
65_G182_A0.6170.11
45_L117_I0.6150.11
75_L82_K0.6150.11
95_K194_S0.6140.11
90_I170_I0.6140.11
196_L239_V0.6130.11
45_L309_I0.6120.11
63_E82_K0.6110.11
29_Y305_P0.6110.11
15_G172_T0.6090.11
156_G282_E0.6080.11
266_N297_V0.6070.11
42_L148_L0.6070.11
156_G208_A0.6050.11
235_V241_G0.6040.11
111_G285_Q0.6020.11
143_Q147_L0.6010.10
164_Q177_I0.5970.10
210_H219_L0.5970.10
189_G280_L0.5950.10
155_M306_T0.5950.10
247_Q289_R0.5930.10
21_A316_F0.5920.10
69_I149_A0.5910.10
39_V304_A0.5890.10
204_L244_I0.5860.10
162_L221_C0.5860.10
70_D77_C0.5830.10
20_K23_P0.5820.10
24_G63_E0.5820.10
28_V164_Q0.5810.10
22_T121_G0.5770.10
75_L176_T0.5770.10
239_V254_P0.5740.10
137_S288_V0.5730.10
5_R154_G0.5720.10
284_R297_V0.5720.10
92_S168_D0.5700.10
30_I58_E0.5700.10
154_G220_I0.5690.10
26_K59_P0.5680.10
66_V166_G0.5680.10
231_G238_Q0.5680.10
218_A239_V0.5660.10
148_L177_I0.5660.10
34_T301_L0.5650.10
133_D301_L0.5640.10
29_Y194_S0.5630.10
56_E151_V0.5610.10
113_K163_F0.5600.10
25_K196_L0.5590.10
15_G152_G0.5560.10
176_T270_A0.5550.10
27_I192_M0.5540.10
58_E284_R0.5520.10
184_V187_L0.5520.10
67_A187_L0.5520.10
122_D252_N0.5510.10
299_R307_V0.5500.10
269_A281_A0.5500.10
57_G252_N0.5490.10
191_I194_S0.5480.10
44_Q167_R0.5460.10
5_R210_H0.5450.10
7_E174_L0.5450.10
36_P270_A0.5440.10
38_I43_A0.5420.10
226_L311_W0.5410.10
153_M176_T0.5380.10
171_D218_A0.5370.10
44_Q237_A0.5360.10
101_Q151_V0.5340.10
58_E186_A0.5340.10
36_P305_P0.5330.10
279_S297_V0.5320.10
4_I21_A0.5310.10
239_V304_A0.5310.10
18_E22_T0.5300.10
160_A242_V0.5290.10
169_T309_I0.5290.10
75_L270_A0.5280.10
150_K247_Q0.5280.10
214_L271_C0.5250.10
19_L101_Q0.5240.10
191_I197_G0.5230.09
192_M239_V0.5210.09
106_D242_V0.5190.09
8_K239_V0.5180.09
235_V318_Y0.5180.09
58_E177_I0.5180.09
92_S194_S0.5180.09
15_G289_R0.5180.09
93_T108_Y0.5170.09
150_K212_L0.5170.09
74_T295_V0.5160.09
18_E118_T0.5140.09
250_L312_F0.5130.09
167_R186_A0.5130.09
183_F221_C0.5120.09
53_G93_T0.5110.09
68_V308_L0.5100.09
158_T178_L0.5090.09
225_L304_A0.5090.09
250_L317_I0.5080.09
60_A161_V0.5080.09
269_A304_A0.5080.09
173_V177_I0.5070.09
204_L222_S0.5040.09
289_R316_F0.5040.09
136_T141_T0.5000.09
45_L257_L0.5000.09
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4h11A 2 0.2571 20.7 0.982 Contact Map
1uitA 1 0.2853 17.8 0.982 Contact Map
1um1A 1 0.2759 17 0.982 Contact Map
2fneA 2 0.2414 14.5 0.983 Contact Map
1um7A 1 0.2727 14.1 0.983 Contact Map
2csjA 1 0.279 13.1 0.983 Contact Map
4f35D 2 0.6176 11.8 0.983 Contact Map
2v90A 4 0.2539 11.4 0.984 Contact Map
2dazA 1 0.2665 10.5 0.984 Contact Map
2dluA 1 0.2759 9.7 0.984 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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