EMRK - ECOLI - GREMLIN Contacts

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EMRK - Probable multidrug resistance protein EmrK

UniProt:	P52599	Sequences: MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13282
Length:	387 (296)
Sequences:	24010 (18495)
Seq/√Len:	1075.0

ECOLI - EMRK EMRA YIBH YIAV YHII MDTN YBHG MACA ACRA ACRE MDTA MDTE AAEA YDHJ CUSB
BACSU - YVRP YKNX

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
67_V	84_S	3.142	1.00
81_I	210_D	2.906	1.00
77_R	216_T	2.810	1.00
283_T	317_S	2.704	1.00
157_K	161_I	2.410	1.00
69_N	84_S	2.387	1.00
84_S	210_D	2.362	1.00
264_S	279_H	2.357	1.00
133_L	137_N	2.349	1.00
139_R	156_T	2.321	1.00
139_R	148_I	2.312	1.00
160_L	164_K	2.249	1.00
266_N	277_V	2.168	1.00
77_R	80_D	2.102	1.00
87_K	91_T	2.101	1.00
60_Q	208_R	2.093	1.00
266_N	279_H	2.073	1.00
140_V	144_K	2.069	1.00
98_K	203_W	2.067	1.00
75_Y	218_Y	2.067	1.00
143_A	150_K	1.972	1.00
81_I	212_K	1.952	1.00
69_N	81_I	1.933	1.00
78_Q	216_T	1.929	1.00
280_G	316_V	1.913	1.00
140_V	157_K	1.904	1.00
131_Q	135_D	1.903	1.00
248_N	313_P	1.869	1.00
31_A	35_W	1.866	1.00
150_K	154_E	1.859	1.00
246_W	315_E	1.850	1.00
152_T	155_H	1.842	1.00
133_L	160_L	1.838	1.00
220_A	240_V	1.818	1.00
136_Y	140_V	1.792	1.00
101_L	199_T	1.785	1.00
91_T	95_N	1.752	1.00
129_Y	160_L	1.752	1.00
51_T	248_N	1.714	1.00
249_A	336_A	1.712	1.00
133_L	164_K	1.712	1.00
94_L	203_W	1.706	1.00
136_Y	153_L	1.703	1.00
126_R	167_L	1.697	1.00
94_L	98_K	1.660	1.00
143_A	153_L	1.652	1.00
281_R	319_D	1.644	1.00
95_N	98_K	1.627	1.00
136_Y	160_L	1.609	1.00
129_Y	133_L	1.606	1.00
129_Y	164_K	1.598	1.00
71_K	74_N	1.593	1.00
161_I	164_K	1.578	1.00
281_R	317_S	1.571	1.00
249_A	316_V	1.569	1.00
96_K	198_A	1.566	1.00
264_S	281_R	1.556	1.00
153_L	157_K	1.551	1.00
94_L	199_T	1.539	1.00
130_Q	134_E	1.530	1.00
265_V	316_V	1.523	1.00
135_D	155_H	1.518	1.00
247_V	334_M	1.511	1.00
91_T	206_L	1.504	1.00
136_Y	157_K	1.504	1.00
30_G	34_Y	1.500	1.00
155_H	159_T	1.495	1.00
31_A	34_Y	1.467	1.00
129_Y	167_L	1.466	1.00
164_K	168_N	1.464	1.00
93_A	96_K	1.457	1.00
284_G	315_E	1.457	1.00
231_S	234_Q	1.451	1.00
66_T	86_D	1.449	1.00
289_T	312_V	1.440	1.00
65_V	224_V	1.424	1.00
193_V	196_A	1.414	1.00
65_V	83_V	1.414	1.00
159_T	162_S	1.410	1.00
200_K	203_W	1.410	1.00
201_E	205_A	1.409	1.00
266_N	339_D	1.403	1.00
98_K	199_T	1.402	1.00
32_Y	36_S	1.402	1.00
268_I	337_T	1.391	1.00
244_Q	317_S	1.379	1.00
66_T	84_S	1.378	1.00
101_L	196_A	1.372	1.00
123_A	126_R	1.371	1.00
63_G	85_L	1.370	1.00
81_I	84_S	1.364	1.00
128_Q	162_S	1.361	1.00
75_Y	242_A	1.348	1.00
96_K	100_N	1.348	1.00
132_S	163_S	1.346	1.00
269_S	334_M	1.333	1.00
126_R	130_Q	1.331	1.00
140_V	153_L	1.321	1.00
151_E	155_H	1.302	1.00
93_A	201_E	1.301	1.00
137_N	141_P	1.299	1.00
16_Y	20_L	1.296	1.00
54_A	238_A	1.290	1.00
125_A	170_A	1.285	1.00
199_T	203_W	1.280	1.00
93_A	205_A	1.276	1.00
141_P	145_Q	1.276	1.00
250_N	313_P	1.275	1.00
130_Q	133_L	1.269	1.00
276_V	327_P	1.268	1.00
219_I	237_M	1.267	1.00
196_A	200_K	1.259	1.00
266_N	337_T	1.239	1.00
100_N	103_N	1.238	1.00
322_E	327_P	1.235	1.00
56_P	238_A	1.231	1.00
141_P	144_K	1.228	1.00
33_A	37_M	1.221	1.00
70_H	80_D	1.215	1.00
67_V	226_V	1.213	1.00
137_N	140_V	1.201	1.00
213_S	239_V	1.197	1.00
130_Q	161_I	1.194	1.00
53_N	241_P	1.193	1.00
138_R	154_E	1.186	1.00
204_L	208_R	1.184	1.00
252_K	255_Q	1.177	1.00
248_N	315_E	1.175	1.00
83_V	211_I	1.175	1.00
98_K	102_A	1.174	1.00
241_P	244_Q	1.162	1.00
62_S	232_P	1.156	1.00
29_S	33_A	1.152	1.00
203_W	207_K	1.149	1.00
166_A	169_A	1.147	1.00
135_D	151_E	1.141	1.00
95_N	200_K	1.140	1.00
94_L	206_L	1.125	1.00
191_Q	194_E	1.118	1.00
268_I	277_V	1.116	1.00
221_Q	238_A	1.114	1.00
56_P	235_S	1.110	1.00
82_L	237_M	1.105	1.00
154_E	157_K	1.099	1.00
159_T	163_S	1.095	1.00
124_S	166_A	1.084	1.00
134_E	154_E	1.082	1.00
128_Q	131_Q	1.075	1.00
90_A	209_T	1.073	1.00
60_Q	64_S	1.072	1.00
125_A	128_Q	1.068	1.00
55_D	215_V	1.060	1.00
157_K	160_L	1.053	1.00
135_D	139_R	1.045	1.00
125_A	163_S	1.041	1.00
87_K	209_T	1.037	1.00
131_Q	158_D	1.033	1.00
34_Y	37_M	1.032	1.00
217_G	239_V	1.032	1.00
122_V	126_R	1.027	1.00
357_S	362_T	1.019	1.00
162_S	166_A	1.006	1.00
131_Q	155_H	1.002	1.00
222_R	225_Q	1.000	1.00
286_N	313_P	0.998	1.00
72_D	221_Q	0.995	1.00
59_A	211_I	0.991	1.00
87_K	210_D	0.990	1.00
260_R	263_Q	0.975	1.00
64_S	229_T	0.966	1.00
230_V	236_L	0.963	1.00
88_T	204_L	0.958	1.00
279_H	319_D	0.957	1.00
121_E	124_S	0.956	1.00
50_V	247_V	0.951	1.00
100_N	198_A	0.947	1.00
28_F	32_Y	0.946	1.00
128_Q	166_A	0.944	1.00
247_V	267_I	0.942	1.00
75_Y	216_T	0.938	1.00
70_H	237_M	0.937	1.00
146_G	150_K	0.932	1.00
68_V	226_V	0.929	1.00
198_A	201_E	0.929	1.00
246_W	317_S	0.923	1.00
83_V	237_M	0.922	1.00
282_V	314_V	0.920	1.00
71_K	222_R	0.919	1.00
34_Y	38_E	0.917	1.00
100_N	194_E	0.916	1.00
268_I	335_T	0.916	1.00
267_I	318_L	0.914	1.00
124_S	128_Q	0.908	1.00
139_R	152_T	0.905	1.00
89_D	205_A	0.903	1.00
122_V	167_L	0.903	1.00
74_N	80_D	0.901	1.00
251_F	314_V	0.899	1.00
134_E	137_N	0.894	1.00
246_W	283_T	0.894	1.00
83_V	236_L	0.893	1.00
204_L	207_K	0.891	1.00
264_S	339_D	0.885	1.00
45_T	337_T	0.880	1.00
43_I	337_T	0.878	1.00
189_Q	193_V	0.876	1.00
65_V	230_V	0.875	1.00
119_S	123_A	0.873	1.00
192_V	196_A	0.868	1.00
156_T	159_T	0.861	1.00
213_S	216_T	0.855	1.00
93_A	97_A	0.855	1.00
62_S	231_S	0.851	1.00
101_L	192_V	0.850	1.00
268_I	274_E	0.850	1.00
68_V	83_V	0.848	1.00
83_V	222_R	0.843	1.00
84_S	87_K	0.841	1.00
166_A	170_A	0.840	1.00
223_S	238_A	0.839	1.00
343_E	346_A	0.839	1.00
251_F	259_V	0.838	1.00
45_T	335_T	0.836	1.00
339_D	342_N	0.835	1.00
256_L	312_V	0.835	1.00
269_S	272_Y	0.833	1.00
282_V	316_V	0.832	1.00
48_A	247_V	0.831	1.00
247_V	336_A	0.827	1.00
103_N	191_Q	0.822	1.00
92_I	204_L	0.822	1.00
349_P	363_S	0.817	1.00
92_I	197_D	0.815	1.00
99_N	197_D	0.814	1.00
152_T	156_T	0.814	1.00
148_I	152_T	0.812	1.00
194_E	198_A	0.808	1.00
197_D	200_K	0.806	1.00
272_Y	276_V	0.804	1.00
128_Q	132_S	0.798	1.00
126_R	129_Y	0.794	1.00
143_A	148_I	0.793	1.00
57_I	214_P	0.789	1.00
355_V	362_T	0.787	1.00
33_A	36_S	0.786	1.00
128_Q	159_T	0.785	1.00
59_A	230_V	0.782	1.00
27_A	31_A	0.780	1.00
62_S	229_T	0.775	1.00
35_W	38_E	0.773	1.00
150_K	153_L	0.770	1.00
128_Q	163_S	0.770	1.00
66_T	69_N	0.770	1.00
267_I	280_G	0.769	1.00
93_A	202_A	0.768	1.00
290_G	293_F	0.767	1.00
70_H	219_I	0.765	1.00
91_T	94_L	0.765	1.00
97_A	202_A	0.760	1.00
50_V	245_M	0.759	1.00
163_S	166_A	0.759	1.00
66_T	87_K	0.758	1.00
90_A	94_L	0.756	1.00
250_N	311_R	0.756	1.00
222_R	237_M	0.753	1.00
36_S	39_L	0.752	1.00
98_K	101_L	0.752	1.00
158_D	162_S	0.751	1.00
97_A	100_N	0.750	1.00
94_L	202_A	0.749	1.00
191_Q	195_A	0.748	1.00
259_V	338_I	0.748	1.00
163_S	167_L	0.747	1.00
32_Y	35_W	0.746	1.00
75_Y	326_H	0.746	1.00
267_I	316_V	0.739	1.00
59_A	83_V	0.736	1.00
202_A	205_A	0.733	1.00
64_S	86_D	0.732	1.00
53_N	225_Q	0.729	1.00
125_A	166_A	0.728	1.00
20_L	24_L	0.727	1.00
70_H	222_R	0.726	1.00
96_K	201_E	0.719	1.00
17_F	21_A	0.717	1.00
60_Q	89_D	0.716	1.00
273_G	276_V	0.716	1.00
290_G	313_P	0.707	1.00
78_Q	214_P	0.707	1.00
89_D	93_A	0.707	1.00
140_V	143_A	0.705	1.00
16_Y	19_L	0.701	1.00
251_F	338_I	0.701	1.00
38_E	41_D	0.700	1.00
207_K	212_K	0.699	1.00
90_A	93_A	0.699	1.00
87_K	206_L	0.699	1.00
121_E	169_A	0.697	1.00
249_A	338_I	0.697	1.00
52_G	244_Q	0.697	1.00
121_E	125_A	0.696	1.00
267_I	334_M	0.695	1.00
102_A	193_V	0.694	1.00
30_G	33_A	0.693	1.00
76_V	82_L	0.690	1.00
44_S	48_A	0.690	1.00
132_S	156_T	0.687	1.00
24_L	28_F	0.686	1.00
218_Y	242_A	0.683	1.00
272_Y	278_F	0.683	1.00
72_D	222_R	0.682	1.00
18_S	22_V	0.682	1.00
223_S	235_S	0.681	1.00
132_S	159_T	0.680	1.00
245_M	330_I	0.680	1.00
15_K	18_S	0.678	1.00
118_Y	121_E	0.677	1.00
255_Q	258_D	0.673	1.00
143_A	146_G	0.673	1.00
118_Y	170_A	0.673	1.00
103_N	166_A	0.668	1.00
196_A	199_T	0.666	1.00
215_V	239_V	0.665	1.00
97_A	101_L	0.664	1.00
57_I	237_M	0.661	1.00
92_I	95_N	0.661	1.00
97_A	195_A	0.660	1.00
120_A	123_A	0.659	1.00
61_V	90_A	0.651	1.00
59_A	62_S	0.650	1.00
127_I	131_Q	0.649	1.00
278_F	322_E	0.644	1.00
135_D	156_T	0.643	1.00
247_V	318_L	0.641	1.00
15_K	19_L	0.640	1.00
59_A	236_L	0.640	1.00
205_A	208_R	0.639	1.00
164_K	167_L	0.638	1.00
320_P	326_H	0.631	1.00
52_G	246_W	0.630	1.00
271_L	332_L	0.630	1.00
131_Q	134_E	0.629	1.00
119_S	122_V	0.628	1.00
29_S	32_Y	0.628	1.00
17_F	20_L	0.627	1.00
35_W	39_L	0.626	1.00
95_N	99_N	0.623	1.00
90_A	202_A	0.623	1.00
37_M	40_E	0.621	1.00
207_K	210_D	0.619	1.00
195_A	199_T	0.614	1.00
19_L	23_V	0.613	1.00
259_V	282_V	0.612	1.00
61_V	205_A	0.612	1.00
200_K	204_L	0.611	1.00
150_K	157_K	0.611	1.00
50_V	328_L	0.611	1.00
131_Q	159_T	0.608	1.00
29_S	34_Y	0.607	1.00
161_I	165_A	0.607	1.00
54_A	240_V	0.605	1.00
70_H	74_N	0.605	1.00
262_G	281_R	0.604	1.00
283_T	315_E	0.604	1.00
132_S	135_D	0.601	1.00
139_R	151_E	0.600	1.00
165_A	168_N	0.599	1.00
329_R	332_L	0.598	1.00
195_A	198_A	0.598	1.00
235_S	238_A	0.597	1.00
23_V	27_A	0.597	1.00
211_I	236_L	0.597	1.00
129_Y	163_S	0.597	1.00
135_D	159_T	0.594	1.00
125_A	167_L	0.593	1.00
137_N	160_L	0.590	1.00
263_Q	340_T	0.590	1.00
58_S	229_T	0.584	1.00
94_L	97_A	0.581	1.00
127_I	130_Q	0.579	1.00
355_V	364_K	0.577	1.00
49_Y	248_N	0.574	1.00
349_P	353_S	0.571	1.00
148_I	153_L	0.571	1.00
201_E	204_L	0.570	1.00
189_Q	192_V	0.568	1.00
261_I	285_I	0.568	1.00
59_A	85_L	0.567	1.00
261_I	283_T	0.566	1.00
265_V	280_G	0.566	1.00
270_D	335_T	0.564	1.00
72_D	315_E	0.562	1.00
151_E	154_E	0.561	1.00
240_V	330_I	0.561	1.00
154_E	158_D	0.558	1.00
89_D	208_R	0.556	1.00
56_P	228_E	0.556	1.00
89_D	204_L	0.555	1.00
99_N	102_A	0.554	1.00
52_G	241_P	0.553	1.00
88_T	207_K	0.553	1.00
139_R	143_A	0.552	1.00
28_F	34_Y	0.551	1.00
55_D	214_P	0.547	1.00
36_S	40_E	0.545	1.00
123_A	127_I	0.545	1.00
55_D	239_V	0.545	1.00
122_V	170_A	0.544	1.00
68_V	222_R	0.543	1.00
267_I	336_A	0.543	1.00
265_V	338_I	0.542	1.00
52_G	317_S	0.542	1.00
316_V	336_A	0.541	1.00
351_L	354_T	0.540	1.00
61_V	208_R	0.540	1.00
79_G	212_K	0.540	1.00
118_Y	122_V	0.539	1.00
35_W	40_E	0.537	1.00
65_V	236_L	0.537	1.00
19_L	22_V	0.534	1.00
48_A	334_M	0.531	1.00
51_T	246_W	0.528	1.00
249_A	314_V	0.528	1.00
350_E	353_S	0.527	1.00
121_E	166_A	0.524	1.00
134_E	138_R	0.524	1.00
149_S	152_T	0.521	1.00
251_F	336_A	0.519	1.00
66_T	226_V	0.518	1.00
158_D	161_I	0.516	1.00
73_T	284_G	0.514	1.00
224_V	228_E	0.514	1.00
92_I	201_E	0.513	1.00
147_V	151_E	0.512	1.00
138_R	151_E	0.511	1.00
336_A	339_D	0.511	1.00
55_D	59_A	0.510	1.00
202_A	206_L	0.506	1.00
190_P	193_V	0.504	1.00
198_A	202_A	0.504	1.00
100_N	191_Q	0.503	1.00
69_N	80_D	0.502	1.00
228_E	234_Q	0.502	1.00
312_V	332_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4dk0A	5	0.7855	100	0.334	Contact Map
3fppA	3	0.6796	100	0.337	Contact Map
4tkoB	1	0.7235	100	0.365	Contact Map
3lnnA	6	0.814	100	0.37	Contact Map
1vf7A	5	0.5814	100	0.401	Contact Map
4l8jA	2	0.7287	100	0.416	Contact Map
2f1mA	2	0.6021	100	0.421	Contact Map
3ne5B	3	0.6693	100	0.423	Contact Map
4kkuA	1	0.5762	100	0.474	Contact Map
2k32A	1	0.2972	99.8	0.694	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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