GREMLIN Database
GSPJ - Putative type II secretion system protein J
UniProt: P45761 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12893
Length: 195 (170)
Sequences: 740 (566)
Seq/√Len: 43.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
157_T169_Q4.8941.00
62_L83_A3.1511.00
7_G10_L3.0421.00
71_R123_D3.0121.00
47_Q50_Q3.0001.00
113_T132_A2.9761.00
107_W133_M2.8971.00
58_L158_L2.8621.00
68_R123_D2.7981.00
55_I170_R2.6121.00
32_S36_E2.5161.00
26_L30_I2.4061.00
58_L62_L2.3771.00
68_R71_R2.3621.00
61_D116_R2.3351.00
159_H167_G2.1701.00
108_Y115_Y2.0500.99
104_T119_R2.0150.99
38_H42_Q1.9490.99
108_Y117_A1.9320.99
47_Q164_Q1.9140.99
105_V116_R1.9030.99
88_Q92_P1.9020.99
7_G12_E1.8990.99
159_H169_Q1.7750.98
54_T114_L1.7700.98
143_E146_S1.7360.98
20_F24_S1.7340.98
9_T12_E1.7280.98
84_I104_T1.7150.98
34_A38_H1.6530.97
48_F52_Q1.6240.97
54_T134_L1.6210.97
103_L118_V1.5890.96
88_Q91_D1.5890.96
91_D97_S1.5720.96
55_I172_F1.5380.95
139_S161_Q1.5350.95
19_I53_R1.5300.95
107_W160_L1.5120.95
86_T102_L1.4390.94
113_T135_E1.4330.93
27_A30_I1.4200.93
21_S51_L1.4070.93
23_L27_A1.3930.92
50_Q54_T1.3870.92
28_F57_I1.3780.92
110_R115_Y1.3360.91
46_Q133_M1.3250.90
141_L159_H1.3140.90
17_L24_S1.3080.90
59_D170_R1.3020.90
138_E163_Q1.2880.89
28_F155_S1.2870.89
8_F12_E1.2830.89
13_V18_A1.2790.89
26_L89_S1.2470.87
23_L44_E1.2440.87
22_M26_L1.2390.87
66_V69_R1.2300.87
71_R88_Q1.2280.87
28_F34_A1.2080.86
50_Q135_E1.2020.85
146_S153_P1.1930.85
14_M145_N1.1570.83
89_S118_V1.1490.83
100_Q122_V1.1390.82
59_D172_F1.1340.82
23_L30_I1.1320.82
106_H119_R1.1270.81
28_F32_S1.0830.79
104_T122_V1.0700.78
162_T165_Y1.0650.78
19_I106_H1.0570.77
28_F146_S1.0510.77
58_L114_L1.0450.76
15_A42_Q1.0320.75
93_L124_G1.0180.74
54_T133_M1.0030.73
89_S97_S1.0030.73
16_A31_F0.9960.73
18_A170_R0.9920.72
75_K78_V0.9640.70
76_I152_L0.9560.70
85_F172_F0.9540.69
105_V129_P0.9450.69
48_F134_L0.9440.69
9_T59_D0.9430.68
45_I168_L0.9420.68
87_T175_P0.9370.68
58_L107_W0.9330.68
19_I162_T0.9300.67
29_M33_Q0.9260.67
7_G11_L0.9240.67
18_A21_S0.9120.66
37_L175_P0.9110.66
25_V113_T0.9110.66
97_S100_Q0.9060.65
62_L156_V0.9020.65
70_N86_T0.8770.63
46_Q60_N0.8760.63
70_N122_V0.8650.62
119_R157_T0.8620.61
41_S65_L0.8590.61
84_I119_R0.8440.60
161_Q166_G0.8420.60
60_N174_L0.8340.59
48_F116_R0.8310.59
25_V137_V0.8300.59
57_I155_S0.8280.58
74_D78_V0.8220.58
104_T155_S0.8200.58
110_R140_F0.8140.57
162_T166_G0.8120.57
42_Q133_M0.8040.56
28_F33_Q0.7970.55
22_M136_H0.7970.55
14_M21_S0.7900.55
20_F155_S0.7890.55
89_S92_P0.7840.54
19_I32_S0.7770.54
20_F48_F0.7770.54
47_Q109_L0.7750.53
138_E150_Q0.7720.53
47_Q166_G0.7690.53
43_K154_L0.7690.53
140_F158_L0.7650.52
93_L120_T0.7640.52
52_Q92_P0.7630.52
118_V170_R0.7610.52
28_F121_S0.7530.51
109_L138_E0.7480.51
83_A156_V0.7470.51
17_L155_S0.7410.50
85_F107_W0.7390.50
29_M32_S0.7370.50
20_F52_Q0.7360.50
41_S48_F0.7310.49
13_V32_S0.7220.48
138_E161_Q0.7210.48
16_A21_S0.7180.48
108_Y158_L0.7170.48
11_L119_R0.7160.48
155_S173_A0.7150.48
58_L133_M0.7150.48
15_A84_I0.7090.47
67_A148_E0.7070.47
15_A87_T0.7040.47
11_L27_A0.7020.46
17_L72_S0.7010.46
73_T80_G0.6940.46
76_I83_A0.6880.45
39_Q69_R0.6870.45
57_I107_W0.6840.45
112_H138_E0.6800.44
36_E40_R0.6770.44
13_V173_A0.6750.44
102_L127_D0.6740.44
17_L105_V0.6720.44
27_A49_N0.6680.43
70_N156_V0.6660.43
91_D103_L0.6610.43
11_L20_F0.6580.42
161_Q164_Q0.6580.42
33_Q85_F0.6580.42
21_S24_S0.6570.42
132_A135_E0.6550.42
26_L29_M0.6550.42
135_E141_L0.6550.42
120_T148_E0.6520.42
34_A45_I0.6520.42
34_A55_I0.6520.42
13_V112_H0.6480.41
31_F61_D0.6450.41
44_E48_F0.6440.41
109_L114_L0.6440.41
17_L69_R0.6400.41
22_M158_L0.6370.40
33_Q55_I0.6330.40
169_Q172_F0.6330.40
14_M42_Q0.6270.39
15_A30_I0.6260.39
165_Y168_L0.6250.39
97_S121_S0.6200.39
50_Q147_G0.6190.39
37_L126_K0.6190.39
112_H156_V0.6160.38
72_S152_L0.6140.38
119_R155_S0.6130.38
115_Y132_A0.5990.37
22_M37_L0.5990.37
65_L85_F0.5970.37
137_V162_T0.5960.37
18_A83_A0.5940.36
21_S28_F0.5930.36
4_R76_I0.5880.36
81_E158_L0.5860.36
4_R147_G0.5850.36
59_D89_S0.5830.35
33_Q155_S0.5820.35
30_I57_I0.5810.35
135_E162_T0.5800.35
68_R122_V0.5790.35
83_A148_E0.5790.35
6_Q113_T0.5790.35
80_G138_E0.5760.35
117_A171_R0.5730.35
25_V33_Q0.5730.35
90_R100_Q0.5720.34
86_T122_V0.5710.34
33_Q36_E0.5680.34
143_E152_L0.5670.34
86_T109_L0.5660.34
14_M71_R0.5650.34
13_V117_A0.5590.33
65_L156_V0.5580.33
34_A115_Y0.5580.33
55_I126_K0.5570.33
48_F64_Q0.5570.33
108_Y127_D0.5570.33
129_P144_S0.5530.33
34_A40_R0.5530.33
107_W116_R0.5510.33
80_G140_F0.5490.33
86_T119_R0.5470.32
76_I153_P0.5470.32
46_Q174_L0.5450.32
59_D63_L0.5430.32
161_Q167_G0.5430.32
54_T92_P0.5420.32
35_S66_V0.5420.32
31_F87_T0.5410.32
6_Q13_V0.5410.32
36_E43_K0.5400.32
122_V125_R0.5390.32
145_N168_L0.5340.31
15_A45_I0.5320.31
72_S124_G0.5310.31
139_S158_L0.5300.31
114_L132_A0.5300.31
4_R74_D0.5290.31
47_Q162_T0.5250.31
117_A140_F0.5240.30
58_L117_A0.5210.30
41_S170_R0.5170.30
31_F41_S0.5140.30
68_R88_Q0.5130.30
156_V167_G0.5130.30
6_Q138_E0.5110.29
20_F49_N0.5110.29
87_T91_D0.5100.29
131_Q156_V0.5080.29
65_L133_M0.5030.29
14_M149_S0.5020.29
112_H139_S0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3njeA 1 0.7077 100 0.448 Contact Map
2retB 2 0.7077 100 0.496 Contact Map
3ci0J 1 0.6718 100 0.522 Contact Map
2m7gA 1 0.3128 97.7 0.852 Contact Map
1oqwA 1 0.6923 95.7 0.884 Contact Map
3sokA 1 0.7026 95.6 0.885 Contact Map
2hi2A 1 0.6769 95.6 0.885 Contact Map
4h33A 3 0.1897 9 0.954 Contact Map
3pjsK 4 0.3436 6.2 0.957 Contact Map
3effK 4 0.3436 6.1 0.957 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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