GREMLIN Database
GSPE - Putative type II secretion system protein E
UniProt: P45759 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12891
Length: 493 (468)
Sequences: 4266 (2920)
Seq/√Len: 135.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
436_T458_Q4.9461.00
381_L412_P4.9011.00
234_L262_I4.1611.00
237_L371_G4.0321.00
387_Q413_V4.0181.00
206_R272_N3.8531.00
386_R410_G3.7101.00
442_A455_L3.6591.00
161_I207_A3.5951.00
347_S350_G3.3541.00
384_Q417_H3.3471.00
388_F408_T3.3041.00
271_R314_V3.2561.00
235_E265_A3.1221.00
387_Q411_T2.9661.00
53_L58_F2.8381.00
467_G481_V2.7871.00
389_T411_T2.7821.00
381_L477_S2.7701.00
246_L341_S2.7521.00
9_A13_L2.7491.00
274_L307_I2.7051.00
381_L386_R2.6571.00
442_A447_V2.6341.00
456_V465_K2.6091.00
276_V303_G2.6071.00
380_R427_M2.5651.00
190_R213_D2.4981.00
389_T409_I2.4731.00
202_I280_V2.4471.00
194_V209_L2.4101.00
248_T343_L2.4001.00
238_I262_I2.3881.00
199_L290_T2.3821.00
320_I329_A2.3471.00
231_K235_E2.3321.00
435_V443_I2.3111.00
392_S395_Q2.2491.00
427_M475_D2.2221.00
112_I160_L2.2181.00
466_N470_K2.2151.00
373_I432_M2.2101.00
136_M157_A2.2041.00
177_I296_V2.1841.00
26_I49_T2.1461.00
438_E458_Q2.1431.00
22_S46_Q2.0881.00
206_R288_G2.0881.00
236_N434_V2.0831.00
123_T424_Q2.0571.00
54_S57_D2.0531.00
412_P477_S2.0161.00
170_L198_T2.0011.00
347_S453_E1.9971.00
461_K469_Q1.9901.00
168_A196_V1.9481.00
197_S310_Q1.9391.00
439_L452_L1.9391.00
352_V369_L1.9201.00
145_V379_R1.9191.00
30_A53_L1.9141.00
12_A66_F1.9091.00
148_T151_Q1.9061.00
349_S433_M1.8761.00
391_V409_I1.8471.00
230_D430_H1.8251.00
40_I48_Q1.8211.00
212_L282_Y1.8191.00
441_A445_E1.8091.00
11_W15_Q1.7961.00
229_A461_K1.7861.00
145_V483_R1.7801.00
468_L472_I1.7701.00
194_V211_L1.7631.00
137_S148_T1.7491.00
135_T151_Q1.7321.00
230_D432_M1.7131.00
469_Q473_S1.7021.00
415_C418_C1.6961.00
436_T455_L1.6931.00
382_C385_C1.6901.00
266_L314_V1.6831.00
356_R365_L1.6741.00
228_A232_Q1.6671.00
448_D451_A1.6651.00
240_L370_A1.6631.00
427_M470_K1.6391.00
384_Q424_Q1.6351.00
201_S288_G1.6291.00
385_C418_C1.6261.00
226_M430_H1.6251.00
230_D466_N1.6231.00
198_T207_A1.6161.00
227_T230_D1.6151.00
464_I485_A1.5991.00
164_I196_V1.5871.00
382_C418_C1.5861.00
245_I338_L1.5821.00
385_C415_C1.5711.00
299_S302_R1.5551.00
20_L51_T1.5531.00
28_Y51_T1.5521.00
383_P386_R1.5421.00
447_V451_A1.5411.00
389_T395_Q1.5351.00
293_N296_V1.5311.00
276_V307_I1.5141.00
455_L458_Q1.4961.00
29_L40_I1.4941.00
140_F149_I1.4931.00
433_M460_H1.4911.00
454_R457_R1.4811.00
124_A142_I1.4801.00
290_T307_I1.4791.00
7_Y12_A1.4721.00
409_I471_V1.4671.00
129_I136_M1.4661.00
130_E139_R1.4651.00
462_A465_K1.4631.00
382_C415_C1.4621.00
327_Q331_Q1.4611.00
388_F410_G1.4481.00
21_Y28_Y1.4481.00
266_L340_L1.4351.00
470_K475_D1.4321.00
230_D233_D1.4271.00
34_F38_L1.4271.00
471_V481_V1.4141.00
12_A62_L1.4081.00
438_E457_R1.4001.00
18_G58_F1.3841.00
148_T269_P1.3781.00
112_I164_I1.3721.00
246_L320_I1.3721.00
16_R59_E1.3661.00
25_E46_Q1.3571.00
16_R55_Q1.3551.00
381_L413_V1.3531.00
320_I341_S1.3531.00
267_N273_I1.3421.00
206_R274_L1.3301.00
145_V270_G1.3281.00
445_E451_A1.3111.00
237_L432_M1.3091.00
245_I371_G1.3061.00
27_I44_V1.2971.00
326_A360_V1.2941.00
232_Q236_N1.2931.00
423_Y476_T1.2921.00
13_L63_E1.2911.00
37_L50_M1.2891.00
250_P376_R1.2861.00
437_P440_R1.2831.00
381_L410_G1.2831.00
220_S223_N1.2771.00
411_T479_D1.2711.00
393_P397_Q1.2691.00
248_T345_T1.2681.00
18_G61_R1.2561.00
239_Q265_A1.2501.00
20_L30_A1.2361.00
303_G307_I1.2321.00
372_I433_M1.2271.00
229_A233_D1.2241.00
330_V360_V1.2221.00
251_T312_P1.2211.00
226_M258_T1.2201.00
233_D434_V1.2131.00
349_S452_L1.2081.00
378_V476_T1.1880.99
222_N231_K1.1880.99
244_I339_V1.1870.99
334_L364_L1.1840.99
118_E122_E1.1750.99
331_Q357_D1.1740.99
222_N235_E1.1730.99
183_I194_V1.1700.99
185_L194_V1.1630.99
251_T337_H1.1620.99
267_N286_G1.1410.99
374_A463_L1.1370.99
167_M183_I1.1340.99
142_I147_R1.1320.99
429_I467_G1.1260.99
226_M234_L1.1170.99
351_A372_I1.1160.99
115_I138_I1.1100.99
180_D197_S1.1090.99
259_L316_M1.1060.99
55_Q59_E1.0950.99
331_Q334_L1.0930.99
391_V396_A1.0930.99
41_Q47_C1.0920.99
456_V460_H1.0910.99
356_R362_S1.0850.99
120_I192_I1.0840.99
28_Y49_T1.0780.99
226_M428_A1.0700.99
423_Y477_S1.0680.99
407_T472_I1.0670.99
476_T480_E1.0660.99
236_N240_L1.0650.99
7_Y40_I1.0530.99
451_A454_R1.0530.99
26_I47_C1.0510.99
230_D373_I1.0490.99
60_A64_A1.0450.99
323_T327_Q1.0430.99
379_R480_E1.0380.99
414_G418_C1.0340.99
165_K205_E1.0330.99
30_A51_T1.0310.99
15_Q36_R1.0280.99
192_I211_L1.0280.99
385_C414_G1.0280.99
227_T469_Q1.0260.98
20_L28_Y1.0220.98
25_E47_C1.0200.98
27_I30_A1.0170.98
227_T473_S1.0130.98
399_F468_L1.0000.98
62_L66_F0.9990.98
349_S460_H0.9990.98
234_L432_M0.9950.98
315_V332_A0.9890.98
472_I475_D0.9870.98
307_I315_V0.9820.98
469_Q472_I0.9800.98
197_S208_V0.9780.98
119_A140_F0.9750.98
36_R40_I0.9740.98
301_A324_E0.9690.98
186_R191_N0.9650.98
115_I127_I0.9640.98
272_N288_G0.9640.98
237_L370_A0.9620.98
17_I36_R0.9570.98
248_T351_A0.9550.98
78_Q81_D0.9550.98
17_I29_L0.9530.98
390_P408_T0.9450.97
365_L369_L0.9440.97
280_V291_Q0.9390.97
276_V292_V0.9380.97
305_R324_E0.9360.97
349_S453_E0.9340.97
154_K157_A0.9280.97
471_V478_W0.9280.97
132_Y137_S0.9250.97
369_L372_I0.9250.97
192_I213_D0.9240.97
109_I163_R0.9220.97
123_T142_I0.9170.97
208_V310_Q0.9170.97
276_V290_T0.9160.97
391_V395_Q0.9160.97
33_P36_R0.9140.97
237_L245_I0.9120.97
44_V47_C0.9110.97
242_H347_S0.9100.97
477_S480_E0.9090.97
238_I340_L0.9050.97
30_A58_F0.9040.97
141_R311_D0.9030.97
268_T271_R0.9020.97
120_I211_L0.8990.97
227_T470_K0.8980.97
58_F62_L0.8950.96
326_A355_L0.8930.96
191_N214_K0.8920.96
274_L288_G0.8890.96
206_R310_Q0.8880.96
170_L207_A0.8870.96
468_L471_V0.8860.96
143_D379_R0.8850.96
17_I40_I0.8780.96
439_L455_L0.8770.96
356_R446_N0.8750.96
177_I298_M0.8740.96
441_A454_R0.8640.96
304_L328_I0.8640.96
115_I129_I0.8630.96
24_G46_Q0.8620.96
247_V258_T0.8550.95
27_I46_Q0.8540.95
116_L194_V0.8530.95
22_S25_E0.8520.95
380_R475_D0.8510.95
395_Q398_M0.8500.95
362_S446_N0.8480.95
320_I326_A0.8470.95
202_I283_E0.8460.95
366_S444_H0.8460.95
297_D302_R0.8450.95
396_A407_T0.8440.95
107_P110_R0.8410.95
266_L273_I0.8400.95
429_I484_V0.8390.95
122_E147_R0.8340.95
294_T298_M0.8340.95
327_Q361_E0.8340.95
8_P11_W0.8330.95
429_I485_A0.8310.95
41_Q48_Q0.8300.95
40_I44_V0.8250.94
256_S260_Y0.8250.94
23_E26_I0.8240.94
31_D52_S0.8240.94
267_N270_G0.8230.94
299_S324_E0.8230.94
122_E419_H0.8230.94
247_V373_I0.8200.94
196_V209_L0.8200.94
371_G432_M0.8200.94
319_E344_H0.8180.94
172_I176_R0.8170.94
442_A451_A0.8150.94
290_T303_G0.8150.94
396_A400_K0.8130.94
197_S306_A0.8120.94
61_R64_A0.8120.94
343_L351_A0.8120.94
15_Q39_D0.8080.94
403_Q406_V0.8060.94
456_V459_Q0.8060.94
39_D42_R0.8010.93
435_V439_L0.8010.93
135_T285_E0.7970.93
221_L231_K0.7970.93
396_A401_Y0.7950.93
480_E483_R0.7940.93
163_R167_M0.7940.93
171_D295_R0.7880.93
120_I187_I0.7860.93
227_T466_N0.7800.93
221_L235_E0.7790.93
376_R463_L0.7720.92
382_C414_G0.7700.92
7_Y19_Y0.7680.92
468_L478_W0.7670.92
470_K473_S0.7660.92
230_D461_K0.7620.92
130_E175_K0.7590.92
274_L290_T0.7570.91
447_V452_L0.7560.91
61_R66_F0.7540.91
37_L41_Q0.7520.91
259_L340_L0.7510.91
242_H370_A0.7490.91
309_R319_E0.7490.91
476_T481_V0.7470.91
349_S449_E0.7460.91
309_R344_H0.7440.91
456_V462_A0.7440.91
304_L307_I0.7440.91
352_V443_I0.7420.91
126_D344_H0.7420.91
24_G49_T0.7410.91
193_D214_K0.7380.90
378_V429_I0.7360.90
466_N469_Q0.7350.90
453_E456_V0.7330.90
170_L197_S0.7290.90
19_Y22_S0.7270.90
125_S208_V0.7240.90
409_I412_P0.7240.90
260_Y284_L0.7240.90
244_I329_A0.7190.89
242_H346_N0.7180.89
199_L310_Q0.7170.89
112_I167_M0.7170.89
23_E28_Y0.7150.89
22_S27_I0.7120.89
190_R215_N0.7110.89
281_E344_H0.7110.89
68_Q72_E0.7100.89
238_I245_I0.7080.88
330_V364_L0.7060.88
272_N310_Q0.7040.88
435_V440_R0.7020.88
220_S265_A0.7000.88
117_S121_K0.6970.88
11_W43_Q0.6950.88
255_K344_H0.6920.87
213_D217_L0.6910.87
342_T345_T0.6910.87
128_H311_D0.6900.87
168_A183_I0.6900.87
25_E28_Y0.6880.87
388_F391_V0.6880.87
323_T359_G0.6880.87
221_L261_A0.6860.87
438_E455_L0.6840.87
16_R58_F0.6830.87
111_L115_I0.6830.87
461_K465_K0.6820.87
305_R322_D0.6800.86
18_G65_V0.6770.86
345_T348_A0.6770.86
11_W17_I0.6770.86
408_T411_T0.6770.86
478_W482_M0.6770.86
467_G476_T0.6720.86
166_V172_I0.6710.86
248_T374_A0.6710.86
264_S267_N0.6710.86
184_S191_N0.6700.86
321_R344_H0.6700.86
318_G344_H0.6700.86
258_T373_I0.6700.86
109_I113_N0.6680.85
199_L306_A0.6680.85
234_L373_I0.6670.85
467_G470_K0.6670.85
360_V365_L0.6670.85
217_L220_S0.6670.85
406_V409_I0.6670.85
57_D60_A0.6670.85
381_L388_F0.6650.85
229_A469_Q0.6630.85
378_V427_M0.6630.85
245_I370_A0.6620.85
126_D309_R0.6620.85
357_D444_H0.6600.85
183_I196_V0.6600.85
182_R193_D0.6590.85
124_A140_F0.6590.85
113_N163_R0.6580.85
395_Q479_D0.6570.84
32_T55_Q0.6560.84
438_E442_A0.6560.84
437_P454_R0.6550.84
88_S92_E0.6530.84
298_M303_G0.6520.84
26_I46_Q0.6500.84
36_R58_F0.6480.84
139_R288_G0.6480.84
381_L387_Q0.6480.84
468_L481_V0.6470.84
12_A19_Y0.6470.84
348_A433_M0.6470.84
255_K319_E0.6470.84
309_R321_R0.6460.83
142_I149_I0.6460.83
126_D319_E0.6460.83
17_I46_Q0.6460.83
164_I209_L0.6450.83
256_S284_L0.6420.83
19_Y44_V0.6420.83
111_L160_L0.6410.83
199_L307_I0.6410.83
416_P420_Q0.6400.83
317_V328_I0.6390.83
308_L332_A0.6390.83
15_Q33_P0.6390.83
237_L434_V0.6370.83
464_I467_G0.6370.83
300_F325_T0.6370.83
112_I163_R0.6360.83
134_K285_E0.6350.82
126_D255_K0.6340.82
307_I317_V0.6330.82
353_T449_E0.6330.82
95_A99_L0.6320.82
251_T313_D0.6310.82
59_E63_E0.6290.82
386_R408_T0.6290.82
433_M439_L0.6270.82
433_M452_L0.6270.82
88_S93_M0.6260.82
302_R306_A0.6260.82
245_I373_I0.6240.81
58_F61_R0.6230.81
9_A66_F0.6230.81
345_T374_A0.6230.81
12_A17_I0.6190.81
294_T299_S0.6190.81
394_Q398_M0.6190.81
164_I207_A0.6180.81
322_D325_T0.6180.81
431_E460_H0.6160.80
229_A232_Q0.6160.80
129_I138_I0.6140.80
122_E140_F0.6130.80
135_T148_T0.6120.80
251_T309_R0.6110.80
281_E319_E0.6110.80
224_L430_H0.6110.80
19_Y29_L0.6100.80
89_L93_M0.6100.80
251_T319_E0.6100.80
121_K188_G0.6090.80
234_L238_I0.6090.80
9_A12_A0.6080.80
141_R313_D0.6080.80
136_M161_I0.6060.79
234_L245_I0.6060.79
7_Y17_I0.6050.79
33_P52_S0.6050.79
46_Q50_M0.6040.79
39_D43_Q0.6040.79
438_E441_A0.6030.79
394_Q397_Q0.6030.79
44_V48_Q0.6030.79
396_A406_V0.6020.79
116_L127_I0.6010.79
251_T321_R0.5980.79
389_T392_S0.5980.79
434_V460_H0.5970.78
131_T161_I0.5960.78
267_N271_R0.5960.78
132_Y135_T0.5960.78
156_L160_L0.5950.78
18_G30_A0.5950.78
379_R423_Y0.5940.78
69_N72_E0.5940.78
14_A39_D0.5940.78
177_I302_R0.5920.78
133_E203_Y0.5910.78
35_E38_L0.5900.78
137_S151_Q0.5900.78
157_A161_I0.5890.77
214_K282_Y0.5880.77
210_R344_H0.5880.77
174_E296_V0.5860.77
384_Q416_P0.5860.77
347_S431_E0.5850.77
429_I470_K0.5850.77
228_A231_K0.5840.77
441_A450_Q0.5820.77
91_E94_P0.5820.77
143_D380_R0.5820.77
366_S443_I0.5800.76
235_E239_Q0.5800.76
126_D279_P0.5790.76
237_L373_I0.5780.76
251_T335_T0.5780.76
290_T298_M0.5770.76
247_V259_L0.5770.76
100_L103_D0.5760.76
296_V302_R0.5760.76
347_S463_L0.5760.76
257_T282_Y0.5760.76
391_V401_Y0.5760.76
208_V311_D0.5750.76
256_S282_Y0.5740.76
273_I287_I0.5740.76
279_P319_E0.5730.76
337_H354_R0.5730.76
11_W14_A0.5720.75
482_M485_A0.5720.75
404_L407_T0.5720.75
88_S91_E0.5710.75
33_P39_D0.5710.75
251_T344_H0.5700.75
7_Y11_W0.5690.75
166_V170_L0.5690.75
241_P346_N0.5680.75
129_I157_A0.5680.75
130_E288_G0.5670.75
203_Y283_E0.5670.75
330_V334_L0.5670.75
171_D177_I0.5660.75
7_Y10_S0.5660.75
449_E453_E0.5660.75
176_R296_V0.5660.75
51_T57_D0.5660.75
278_D291_Q0.5660.75
345_T351_A0.5650.75
383_P417_H0.5630.74
63_E72_E0.5630.74
126_D281_E0.5620.74
177_I297_D0.5620.74
170_L196_V0.5610.74
304_L317_V0.5600.74
266_L271_R0.5600.74
171_D176_R0.5590.74
212_L251_T0.5580.74
276_V328_I0.5570.73
37_L48_Q0.5570.73
335_T344_H0.5570.73
279_P344_H0.5560.73
452_L455_L0.5560.73
29_L50_M0.5550.73
418_C422_G0.5550.73
236_N417_H0.5550.73
136_M160_L0.5550.73
213_D260_Y0.5540.73
362_S443_I0.5540.73
165_K179_Q0.5530.73
33_P37_L0.5520.73
450_Q454_R0.5510.73
101_N104_S0.5510.73
113_N167_M0.5510.73
30_A50_M0.5490.72
244_I371_G0.5490.72
378_V467_G0.5480.72
12_A65_V0.5470.72
165_K181_G0.5470.72
304_L308_L0.5470.72
252_G322_D0.5460.72
130_E272_N0.5450.72
298_M306_A0.5440.72
126_D251_T0.5430.72
126_D321_R0.5430.72
396_A399_F0.5430.72
16_R53_L0.5430.72
315_V337_H0.5410.71
165_K168_A0.5410.71
196_V207_A0.5400.71
122_E142_I0.5400.71
18_G54_S0.5400.71
56_A59_E0.5390.71
126_D210_R0.5390.71
210_R309_R0.5390.71
312_P337_H0.5390.71
249_G318_G0.5380.71
388_F407_T0.5380.71
409_I472_I0.5360.71
349_S353_T0.5360.71
20_L58_F0.5350.71
185_L211_L0.5350.71
143_D424_Q0.5350.71
184_S194_V0.5340.70
227_T461_K0.5330.70
110_R295_R0.5330.70
90_S93_M0.5330.70
137_S269_P0.5320.70
43_Q46_Q0.5320.70
392_S398_M0.5320.70
23_E49_T0.5320.70
319_E335_T0.5310.70
128_H195_R0.5310.70
233_D236_N0.5300.70
249_G336_G0.5300.70
170_L181_G0.5290.70
118_E140_F0.5290.70
210_R319_E0.5290.70
275_T282_Y0.5290.70
210_R321_R0.5280.70
162_S172_I0.5280.70
118_E150_L0.5260.69
53_L57_D0.5250.69
90_S94_P0.5250.69
166_V272_N0.5250.69
195_R344_H0.5240.69
256_S361_E0.5240.69
10_S14_A0.5230.69
102_E105_A0.5230.69
255_K309_R0.5230.69
318_G336_G0.5230.69
68_Q71_G0.5230.69
125_S426_R0.5220.69
30_A61_R0.5210.69
213_D282_Y0.5200.68
180_D346_N0.5200.68
463_L467_G0.5200.68
252_G318_G0.5180.68
116_L185_L0.5180.68
56_A60_A0.5180.68
358_M430_H0.5180.68
284_L287_I0.5170.68
24_G29_L0.5170.68
143_D426_R0.5160.68
263_L287_I0.5160.68
38_L41_Q0.5150.68
222_N228_A0.5150.68
178_P325_T0.5150.68
281_E321_R0.5150.68
59_E62_L0.5140.67
99_L102_E0.5140.67
126_D195_R0.5140.67
244_I330_V0.5130.67
415_C422_G0.5120.67
54_S61_R0.5110.67
93_M97_E0.5100.67
118_E121_K0.5100.67
328_I368_S0.5100.67
335_T346_N0.5090.67
264_S284_L0.5090.67
300_F318_G0.5080.67
40_I54_S0.5080.67
89_L94_P0.5070.66
23_E46_Q0.5070.66
20_L49_T0.5060.66
405_A408_T0.5060.66
213_D216_S0.5060.66
225_G230_D0.5050.66
309_R335_T0.5040.66
384_Q478_W0.5040.66
350_G353_T0.5040.66
334_L368_S0.5040.66
40_I43_Q0.5030.66
225_G428_A0.5030.66
461_K466_N0.5030.66
131_T310_Q0.5020.66
443_I452_L0.5020.66
132_Y286_G0.5020.66
147_R419_H0.5020.66
396_A409_I0.5010.66
467_G471_V0.5010.66
433_M462_A0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4phtA 1 0.8884 100 0.266 Contact Map
4ksrA 3 0.7404 100 0.353 Contact Map
1p9rA 1 0.7667 100 0.377 Contact Map
2ewvA 3 0.6592 100 0.563 Contact Map
3jvvA 3 0.6511 100 0.574 Contact Map
2oap1 3 0.8945 100 0.633 Contact Map
4ihqA 3 0.9229 100 0.675 Contact Map
2eyuA 1 0.4645 100 0.687 Contact Map
4bloA 4 0.5274 100 0.714 Contact Map
2gzaA 3 0.5761 100 0.777 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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