GREMLIN Database
GSPA - Putative general secretion pathway protein A
UniProt: P45756 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12888
Length: 489 (453)
Sequences: 312 (261)
Seq/√Len: 12.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
166_I187_I6.1581.00
314_C324_C4.1721.00
49_T97_S3.7840.99
118_L200_L3.6320.99
354_V366_V3.5180.99
68_N99_C3.4860.98
343_A380_F3.4670.98
28_A142_R3.4330.98
166_I202_G3.3390.98
422_L439_K3.2040.97
359_V364_V3.0280.96
191_C201_A2.9910.96
228_I231_D2.9350.95
334_L341_W2.9130.95
180_T183_V2.8780.95
183_V222_W2.8660.95
364_V380_F2.8150.94
79_W83_E2.6780.92
28_A149_I2.5860.91
19_W65_T2.5630.91
85_A222_W2.5480.90
4_R152_V2.5280.90
440_I455_V2.5050.90
165_S199_A2.4880.89
190_L217_F2.4740.89
108_H130_F2.4380.88
130_F207_L2.4170.88
162_L187_I2.3960.87
54_V149_I2.3810.87
452_D456_G2.3210.85
142_R149_I2.3200.85
86_G108_H2.3070.85
66_D79_W2.2930.85
66_D72_Q2.2700.84
122_I324_C2.2190.83
68_N88_L2.1860.82
276_V279_Q2.1650.81
4_R145_K2.1540.81
142_R197_K2.1200.80
437_V441_K2.0640.78
415_E422_L2.0400.77
303_W418_L2.0240.76
343_A356_V1.9690.74
122_I314_C1.9410.73
107_A147_Q1.9360.73
13_C129_Q1.9350.73
413_W417_M1.9090.72
303_W322_L1.8510.69
28_A54_V1.8450.69
70_A85_A1.8320.69
57_Q108_H1.8120.68
1_M38_Q1.8100.68
70_A131_L1.7710.66
299_L324_C1.7710.66
432_W440_I1.7380.64
107_A123_S1.7370.64
402_I440_I1.7360.64
123_S135_R1.7200.63
419_N422_L1.7050.63
178_L206_R1.7040.63
1_M398_G1.6850.62
415_E427_E1.6800.62
203_E209_Q1.6660.61
103_I138_R1.6590.61
83_E413_W1.6520.60
7_V153_S1.6280.59
248_I251_T1.6260.59
36_A108_H1.6250.59
344_S387_D1.6200.59
119_Q123_S1.6160.58
29_G32_K1.5950.57
123_S147_Q1.5740.56
15_K118_L1.5730.56
92_L108_H1.5720.56
13_C22_C1.5680.56
1_M208_L1.5660.56
26_G32_K1.5640.56
398_G402_I1.5560.55
9_L150_E1.5460.55
171_N204_T1.5460.55
119_Q196_R1.5360.54
100_P131_L1.5340.54
1_M139_K1.5320.54
119_Q147_Q1.5320.54
119_Q135_R1.5270.54
130_F208_L1.5220.54
56_W120_R1.5210.54
42_D168_E1.5090.53
100_P130_F1.5020.53
1_M183_V1.4980.53
66_D114_I1.4960.52
212_E221_Q1.4940.52
204_T207_L1.4850.52
122_I150_E1.4810.52
116_D123_S1.4740.51
214_T218_T1.4730.51
48_I54_V1.4690.51
116_D119_Q1.4620.51
136_P146_K1.4590.51
10_S204_T1.4540.50
82_D86_G1.4540.50
440_I451_T1.4500.50
49_T68_N1.4320.49
54_V142_R1.4300.49
89_L126_P1.4230.49
460_L463_L1.4030.48
81_R149_I1.4020.48
118_L418_L1.3980.47
105_E135_R1.3830.47
185_K253_G1.3720.46
22_C40_Q1.3700.46
462_H465_Q1.3660.46
116_D147_Q1.3650.46
10_S44_H1.3520.45
156_R191_C1.3460.45
8_I172_I1.3340.44
40_Q168_E1.3280.44
57_Q417_M1.3260.44
48_I149_I1.3240.44
26_G29_G1.3230.44
299_L303_W1.3210.44
408_E411_I1.3210.44
85_A162_L1.3200.44
280_D283_K1.3190.44
419_N439_K1.3150.43
81_R142_R1.3100.43
107_A135_R1.3080.43
123_S126_P1.3020.43
118_L130_F1.3010.43
317_A322_L1.2960.42
36_A129_Q1.2930.42
105_E146_K1.2850.42
85_A422_L1.2810.42
148_G156_R1.2800.42
30_S149_I1.2780.41
89_L196_R1.2760.41
65_T122_I1.2750.41
130_F145_K1.2740.41
58_G131_L1.2640.41
125_I137_D1.2630.41
252_C256_L1.2580.41
126_P136_P1.2450.40
415_E465_Q1.2440.40
44_H208_L1.2430.40
51_S204_T1.2430.40
91_A266_V1.2290.39
28_A137_D1.2250.39
123_S196_R1.2240.39
85_A152_V1.2240.39
244_S248_I1.2230.39
116_D135_R1.2070.38
135_R147_Q1.2060.38
35_L421_A1.2010.38
104_I195_R1.1950.37
227_R230_G1.1900.37
323_A394_M1.1870.37
169_G195_R1.1840.37
54_V63_I1.1830.37
19_W122_I1.1820.37
26_G31_G1.1790.37
244_S251_T1.1730.36
415_E439_K1.1670.36
162_L381_E1.1660.36
67_D143_D1.1630.36
188_A204_T1.1590.36
30_S67_D1.1580.36
142_R148_G1.1580.36
116_D137_D1.1570.36
331_L381_E1.1570.36
317_A406_S1.1480.35
444_Q453_G1.1470.35
300_Y317_A1.1460.35
149_I197_K1.1400.35
30_S81_R1.1360.35
22_C204_T1.1350.35
199_A206_R1.1330.35
140_V194_D1.1320.35
249_A252_C1.1290.34
105_E123_S1.1260.34
150_E204_T1.1210.34
388_Y419_N1.1190.34
133_I313_W1.1180.34
135_R196_R1.1140.34
89_L124_L1.1130.34
89_L136_P1.1120.34
90_H124_L1.1120.34
191_C461_V1.1090.33
97_S364_V1.1020.33
86_G130_F1.0950.33
165_S206_R1.0940.33
13_C188_A1.0900.33
119_Q125_I1.0890.33
411_I432_W1.0860.32
158_T161_E1.0810.32
187_I192_R1.0760.32
20_R119_Q1.0760.32
232_N245_G1.0750.32
30_S330_S1.0740.32
325_K461_V1.0720.32
137_D143_D1.0720.32
239_L246_T1.0710.32
356_V446_S1.0630.31
105_E196_R1.0620.31
126_P137_D1.0600.31
125_I135_R1.0570.31
414_L455_V1.0550.31
102_I313_W1.0510.31
120_R146_K1.0460.31
3_T395_S1.0440.31
443_F463_L1.0420.31
124_L136_P1.0390.30
397_E441_K1.0380.30
15_K379_W1.0360.30
131_L308_P1.0340.30
39_L207_L1.0330.30
60_A118_L1.0330.30
87_Q244_S1.0280.30
105_E179_L1.0260.30
348_G407_S1.0250.30
123_S137_D1.0200.30
88_L148_G1.0190.30
242_V423_H1.0180.29
402_I433_R1.0170.29
250_L266_V1.0160.29
30_S142_R1.0150.29
418_L463_L1.0140.29
425_S435_L1.0140.29
22_C59_K1.0140.29
107_A126_P1.0120.29
106_N109_L1.0120.29
33_T105_E1.0080.29
48_I52_L1.0070.29
22_C413_W1.0040.29
220_K388_Y1.0040.29
251_T265_P1.0040.29
19_W150_E1.0020.29
17_Q64_V1.0000.29
260_F425_S0.9980.29
147_Q196_R0.9950.29
90_H93_H0.9950.29
68_N168_E0.9860.28
20_R135_R0.9860.28
1_M57_Q0.9860.28
364_V376_T0.9860.28
186_R223_R0.9830.28
24_L331_L0.9810.28
36_A133_I0.9790.28
343_A384_W0.9770.28
257_L438_E0.9740.28
125_I143_D0.9690.27
83_E150_E0.9690.27
115_L137_D0.9680.27
276_V280_D0.9660.27
3_T35_L0.9630.27
107_A196_R0.9610.27
20_R126_P0.9600.27
185_K288_V0.9530.27
253_G258_S0.9530.27
48_I86_G0.9520.27
135_R146_K0.9500.27
326_S389_L0.9490.27
100_P150_E0.9470.27
402_I432_W0.9440.26
22_C145_K0.9390.26
107_A137_D0.9390.26
235_R263_T0.9380.26
360_G371_Q0.9360.26
22_C150_E0.9340.26
27_E146_K0.9330.26
120_R143_D0.9290.26
45_R191_C0.9270.26
98_R188_A0.9270.26
98_R415_E0.9260.26
12_L21_L0.9230.26
323_A445_K0.9210.26
56_W122_I0.9200.26
20_R116_D0.9190.25
120_R392_W0.9180.25
129_Q204_T0.9170.25
162_L166_I0.9140.25
324_C340_P0.9140.25
126_P135_R0.9130.25
55_S143_D0.9110.25
28_A143_D0.9110.25
16_R46_R0.9090.25
122_I145_K0.9060.25
296_L389_L0.9020.25
247_I251_T0.8990.25
20_R137_D0.8980.25
107_A119_Q0.8950.25
112_R455_V0.8950.25
257_L260_F0.8890.24
28_A135_R0.8890.24
27_E136_P0.8860.24
28_A39_L0.8770.24
58_G226_Y0.8750.24
105_E120_R0.8740.24
103_I161_E0.8740.24
1_M230_G0.8730.24
406_S410_E0.8730.24
1_M418_L0.8710.24
66_D200_L0.8700.24
324_C386_G0.8690.24
1_M130_F0.8680.24
398_G464_W0.8680.24
344_S368_V0.8670.24
148_G200_L0.8660.24
120_R135_R0.8660.24
118_L145_K0.8640.23
1_M44_H0.8630.23
22_C188_A0.8620.23
10_S207_L0.8610.23
15_K415_E0.8580.23
23_Y168_E0.8570.23
399_E462_H0.8540.23
35_L59_K0.8540.23
39_L249_A0.8490.23
8_I35_L0.8480.23
122_I395_S0.8480.23
115_L147_Q0.8460.23
254_W258_S0.8460.23
177_L192_R0.8440.23
79_W114_I0.8420.23
245_G253_G0.8410.23
41_K127_D0.8400.23
12_L42_D0.8400.23
15_K345_L0.8390.23
24_L166_I0.8390.23
20_R196_R0.8340.22
98_R226_Y0.8330.22
131_L213_Q0.8260.22
232_N425_S0.8250.22
24_L99_C0.8250.22
20_R33_T0.8230.22
278_K283_K0.8210.22
100_P208_L0.8200.22
427_E434_P0.8160.22
251_T258_S0.8160.22
353_P369_G0.8160.22
447_H466_V0.8150.22
101_L126_P0.8140.22
139_K364_V0.8120.22
66_D122_I0.8120.22
14_E122_I0.8110.22
68_N422_L0.8110.22
135_R143_D0.8090.21
194_D338_N0.8090.21
91_A310_D0.8090.21
129_Q188_A0.8070.21
5_R14_E0.8050.21
4_R432_W0.8040.21
105_E119_Q0.7950.21
36_A102_I0.7940.21
62_W122_I0.7930.21
63_I129_Q0.7930.21
82_D108_H0.7900.21
402_I414_L0.7900.21
28_A107_A0.7890.21
241_V245_G0.7870.21
115_L123_S0.7850.21
126_P147_Q0.7840.21
106_N165_S0.7810.21
8_I14_E0.7800.21
20_R123_S0.7790.20
299_L345_L0.7770.20
182_R363_S0.7720.20
408_E457_F0.7720.20
150_E208_L0.7700.20
14_E21_L0.7690.20
124_L196_R0.7670.20
56_W105_E0.7670.20
10_S129_Q0.7640.20
354_V423_H0.7640.20
119_Q126_P0.7620.20
184_L378_K0.7590.20
89_L123_S0.7580.20
38_Q44_H0.7570.20
1_M190_L0.7550.20
373_W407_S0.7540.20
130_F137_D0.7530.20
298_V355_V0.7510.20
440_I453_G0.7500.20
217_F396_P0.7470.19
398_G406_S0.7450.19
1_M209_Q0.7440.19
120_R123_S0.7430.19
78_A432_W0.7420.19
192_R363_S0.7400.19
150_E324_C0.7400.19
105_E147_Q0.7400.19
44_H395_S0.7390.19
232_N247_I0.7370.19
52_L60_A0.7370.19
123_S143_D0.7360.19
355_V367_L0.7340.19
101_L207_L0.7340.19
13_C168_E0.7300.19
306_E394_M0.7290.19
339_L393_K0.7290.19
115_L141_E0.7270.19
25_L153_S0.7230.19
247_I257_L0.7220.19
28_A197_K0.7220.19
388_Y425_S0.7210.19
3_T297_S0.7210.19
42_D201_A0.7160.18
21_L137_D0.7160.18
355_V381_E0.7160.18
204_T208_L0.7130.18
55_S392_W0.7130.18
6_E292_D0.7120.18
205_I325_K0.7110.18
39_L142_R0.7080.18
22_C226_Y0.7070.18
67_D125_I0.7060.18
5_R202_G0.7060.18
104_I109_L0.7060.18
19_W79_W0.7050.18
54_V197_K0.7050.18
357_V367_L0.7040.18
19_W324_C0.7040.18
72_Q99_C0.7010.18
261_T425_S0.7000.18
37_Q116_D0.6980.18
37_Q123_S0.6970.18
114_I213_Q0.6950.18
246_T267_P0.6940.18
21_L90_H0.6940.18
78_A433_R0.6920.18
143_D146_K0.6920.18
150_E314_C0.6920.18
67_D105_E0.6920.18
34_W120_R0.6900.18
237_M425_S0.6900.18
451_T454_V0.6880.18
266_V397_E0.6860.18
125_I196_R0.6850.18
81_R148_G0.6850.18
456_G460_L0.6830.17
51_S168_E0.6820.17
162_L383_V0.6810.17
4_R58_G0.6810.17
184_L279_Q0.6800.17
129_Q207_L0.6790.17
1_M99_C0.6780.17
124_L135_R0.6770.17
6_E370_Q0.6760.17
424_I442_Q0.6760.17
7_V171_N0.6750.17
98_R129_Q0.6740.17
9_L13_C0.6740.17
426_T445_K0.6740.17
136_P141_E0.6730.17
305_Y311_S0.6730.17
237_M438_E0.6730.17
340_P386_G0.6720.17
53_V68_N0.6710.17
119_Q169_G0.6700.17
35_L369_G0.6700.17
136_P147_Q0.6690.17
20_R28_A0.6690.17
31_G104_I0.6680.17
65_T150_E0.6680.17
9_L127_D0.6640.17
65_T324_C0.6620.17
61_A178_L0.6620.17
22_C129_Q0.6600.17
39_L100_P0.6590.17
453_G456_G0.6590.17
27_E142_R0.6580.17
79_W122_I0.6560.17
77_S222_W0.6550.17
65_T79_W0.6540.17
197_K245_G0.6540.17
51_S123_S0.6540.17
436_L439_K0.6530.17
464_W467_A0.6530.17
332_Q388_Y0.6510.17
246_T266_V0.6480.16
400_S410_E0.6480.16
19_W120_R0.6470.16
7_V353_P0.6460.16
323_A458_S0.6450.16
122_I151_L0.6450.16
407_S442_Q0.6440.16
108_H133_I0.6440.16
50_L317_A0.6410.16
77_S176_D0.6390.16
189_L217_F0.6390.16
44_H129_Q0.6380.16
411_I433_R0.6370.16
400_S445_K0.6370.16
57_Q92_L0.6360.16
53_V261_T0.6360.16
64_V380_F0.6360.16
303_W340_P0.6350.16
40_Q96_D0.6340.16
252_C278_K0.6340.16
393_K465_Q0.6330.16
249_A298_V0.6330.16
38_Q129_Q0.6330.16
218_T320_A0.6330.16
364_V377_H0.6330.16
28_A52_L0.6320.16
114_I203_E0.6320.16
398_G462_H0.6310.16
9_L83_E0.6300.16
120_R153_S0.6300.16
84_M247_I0.6260.16
14_E56_W0.6240.16
34_W89_L0.6240.16
106_N132_L0.6230.16
14_E152_V0.6220.16
24_L120_R0.6200.16
28_A123_S0.6200.16
138_R204_T0.6190.16
25_L199_A0.6190.16
64_V103_I0.6190.16
406_S445_K0.6180.16
303_W324_C0.6160.16
39_L455_V0.6160.16
418_L442_Q0.6140.16
251_T255_L0.6140.16
7_V455_V0.6130.16
101_L208_L0.6120.16
331_L418_L0.6110.15
155_G433_R0.6100.15
2_S5_R0.6080.15
388_Y422_L0.6080.15
241_V248_I0.6080.15
91_A267_P0.6070.15
143_D153_S0.6060.15
256_L426_T0.6060.15
19_W314_C0.6060.15
14_E151_L0.6030.15
4_R108_H0.6030.15
315_D319_R0.6030.15
240_A252_C0.6020.15
304_G320_A0.6020.15
300_Y305_Y0.6020.15
239_L243_M0.6010.15
299_L314_C0.6000.15
141_E165_S0.5990.15
33_T135_R0.5990.15
123_S146_K0.5980.15
383_V455_V0.5980.15
250_L253_G0.5970.15
150_E168_E0.5960.15
106_N172_I0.5960.15
248_I421_A0.5960.15
119_Q148_G0.5950.15
115_L143_D0.5930.15
335_V359_V0.5920.15
51_S146_K0.5910.15
300_Y415_E0.5900.15
276_V282_T0.5900.15
10_S22_C0.5890.15
131_L207_L0.5880.15
22_C153_S0.5850.15
160_H255_L0.5850.15
51_S143_D0.5840.15
20_R125_I0.5840.15
35_L329_A0.5830.15
357_V365_D0.5830.15
365_D374_T0.5820.15
67_D126_P0.5820.15
117_D122_I0.5810.15
72_Q79_W0.5810.15
129_Q408_E0.5790.15
48_I461_V0.5770.15
252_C319_R0.5770.15
13_C90_H0.5770.15
235_R243_M0.5770.15
24_L39_L0.5760.15
252_C294_E0.5760.15
30_S148_G0.5750.15
16_R227_R0.5730.14
143_D210_Q0.5720.14
67_D124_L0.5700.14
446_S451_T0.5680.14
414_L425_S0.5670.14
207_L211_A0.5670.14
48_I142_R0.5650.14
11_W30_S0.5630.14
314_C452_D0.5630.14
106_N199_A0.5610.14
28_A99_C0.5610.14
77_S411_I0.5610.14
133_I465_Q0.5580.14
22_C58_G0.5570.14
107_A120_R0.5570.14
107_A143_D0.5560.14
57_Q201_A0.5560.14
88_L421_A0.5550.14
86_G124_L0.5550.14
33_T169_G0.5530.14
182_R219_A0.5530.14
49_T99_C0.5520.14
43_K63_I0.5520.14
20_R147_Q0.5520.14
142_R300_Y0.5510.14
223_R252_C0.5500.14
117_D154_I0.5500.14
437_V450_K0.5480.14
35_L133_I0.5460.14
295_A352_L0.5460.14
81_R225_I0.5450.14
96_D195_R0.5450.14
4_R155_G0.5450.14
254_W435_L0.5440.14
59_K200_L0.5430.14
85_A415_E0.5430.14
25_L79_W0.5410.14
98_R197_K0.5400.14
10_S228_I0.5390.14
1_M80_T0.5370.14
444_Q452_D0.5370.14
9_L413_W0.5360.14
1_M52_L0.5360.14
147_Q195_R0.5360.14
235_R376_T0.5350.14
163_K184_L0.5330.14
204_T228_I0.5330.14
116_D126_P0.5320.13
91_A95_T0.5320.13
1_M467_A0.5310.13
119_Q137_D0.5310.13
233_R288_V0.5310.13
36_A207_L0.5310.13
221_Q250_L0.5290.13
166_I427_E0.5290.13
8_I37_Q0.5280.13
120_R136_P0.5270.13
299_L305_Y0.5270.13
303_W393_K0.5260.13
151_L154_I0.5250.13
58_G178_L0.5250.13
63_I118_L0.5240.13
89_L105_E0.5240.13
122_I153_S0.5240.13
394_M445_K0.5240.13
50_L164_A0.5230.13
71_E87_Q0.5220.13
127_D166_I0.5220.13
400_S431_E0.5220.13
303_W314_C0.5210.13
119_Q143_D0.5200.13
340_P384_W0.5190.13
160_H241_V0.5190.13
56_W151_L0.5190.13
7_V11_W0.5170.13
457_F466_V0.5160.13
160_H445_K0.5160.13
185_K327_G0.5150.13
116_D120_R0.5140.13
28_A148_G0.5140.13
422_L436_L0.5130.13
28_A81_R0.5130.13
56_W137_D0.5130.13
306_E355_V0.5120.13
122_I149_I0.5120.13
15_K145_K0.5110.13
296_L398_G0.5110.13
63_I295_A0.5110.13
81_R156_R0.5110.13
296_L317_A0.5100.13
128_G190_L0.5090.13
312_A391_L0.5080.13
28_A48_I0.5080.13
10_S13_C0.5060.13
23_Y57_Q0.5060.13
342_I371_Q0.5050.13
86_G100_P0.5030.13
192_R449_L0.5030.13
25_L165_S0.5020.13
198_L344_S0.5020.13
63_I146_K0.5010.13
60_A155_G0.5010.13
172_I380_F0.5010.13
21_L143_D0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4g54A 1 0.4151 100 0.784 Contact Map
1fnnA 1 0.6646 99.9 0.854 Contact Map
1w5sA 1 0.6748 99.9 0.856 Contact Map
3te6A 1 0.456 99.9 0.856 Contact Map
2qbyA 1 0.6544 99.9 0.86 Contact Map
1njgA 1 0.4417 99.9 0.864 Contact Map
2qp9X 1 0.4438 99.8 0.867 Contact Map
4xgcA 1 0.593 99.8 0.869 Contact Map
2v1uA 1 0.6748 99.8 0.869 Contact Map
2c9oA 3 0.4744 99.8 0.869 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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