GREMLIN Database
YCAL - Uncharacterized metalloprotease YcaL
UniProt: P43674 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13133
Length: 254 (228)
Sequences: 4589 (3425)
Seq/√Len: 226.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
76_R125_D3.8321.00
81_A95_Y3.7011.00
219_E247_R3.3941.00
84_L136_L3.0001.00
127_E220_T2.9881.00
231_S241_E2.9781.00
130_G214_L2.7881.00
115_V132_L2.5871.00
194_S245_H2.5151.00
94_N112_C2.5031.00
194_S241_E2.4311.00
127_E216_G2.4231.00
137_G199_F2.3991.00
99_M117_S2.3491.00
198_D245_H2.3311.00
222_A240_T2.1631.00
216_G219_E2.1541.00
107_A139_V2.1191.00
123_M217_S2.0981.00
141_L199_F2.0671.00
115_V128_I2.0371.00
133_G200_S2.0311.00
73_Y97_V1.9871.00
124_N127_E1.9861.00
70_S73_Y1.9131.00
143_H195_E1.8081.00
245_H248_D1.7581.00
124_N213_G1.7361.00
198_D249_R1.7001.00
147_E192_K1.6781.00
50_K54_N1.6481.00
106_W114_R1.6351.00
77_L115_V1.6181.00
49_A142_G1.6141.00
140_A203_L1.5961.00
57_C96_K1.5921.00
126_N213_G1.5701.00
45_S48_D1.5531.00
60_M98_Y1.5271.00
127_E213_G1.5221.00
244_Q247_R1.5021.00
215_V247_R1.4981.00
27_D30_M1.4971.00
202_D206_K1.4901.00
201_F246_I1.4901.00
130_G213_G1.4811.00
215_V250_I1.4811.00
119_L131_V1.4681.00
244_Q248_D1.4661.00
98_Y114_R1.4591.00
78_A82_K1.4491.00
215_V246_I1.4461.00
191_D194_S1.4231.00
57_C61_D1.4071.00
75_K79_K1.4061.00
123_M128_I1.4011.00
102_D116_Y1.4001.00
219_E243_A1.3781.00
101_S121_D1.3631.00
246_I250_I1.3621.00
212_Q250_I1.3321.00
57_C114_R1.3291.00
137_G141_L1.3211.00
76_R80_I1.3161.00
202_D249_R1.3061.00
49_A53_A1.2991.00
153_A157_A1.2941.00
241_E244_Q1.2901.00
239_S243_A1.2891.00
198_D202_D1.2851.00
149_K153_A1.2791.00
142_G145_L1.2751.00
64_N116_Y1.2741.00
95_Y113_V1.2721.00
182_G187_K1.2581.00
59_E63_G1.2491.00
141_L195_E1.2461.00
130_G204_L1.2431.00
165_S169_S1.2381.00
214_L246_I1.2321.00
222_A239_S1.2281.00
73_Y76_R1.2271.00
126_N210_S1.2101.00
199_F203_L1.1961.00
48_D51_A1.1931.00
71_S75_K1.1921.00
245_H249_R1.1921.00
187_K190_R1.1921.00
240_T244_Q1.1701.00
56_G59_E1.1671.00
205_K211_T1.1671.00
216_G220_T1.1661.00
100_T116_Y1.1631.00
157_A161_I1.1621.00
241_E245_H1.1591.00
130_G217_S1.1501.00
186_A235_S1.1461.00
126_N209_I1.1381.00
38_A146_A1.1281.00
76_R79_K1.1171.00
129_E209_I1.1101.00
80_I129_E1.0731.00
230_Q233_F1.0671.00
195_E199_F1.0671.00
59_E62_S1.0651.00
137_G140_A1.0601.00
109_A144_S1.0581.00
225_D239_S1.0571.00
81_A113_V1.0531.00
234_D238_P1.0451.00
103_V116_Y1.0441.00
26_I29_N1.0441.00
228_R239_S1.0341.00
212_Q247_R1.0311.00
146_A192_K1.0271.00
74_G78_A1.0191.00
53_A110_N1.0151.00
171_L175_Q1.0141.00
150_A192_K1.0111.00
81_A85_G1.0021.00
243_A247_R1.0011.00
79_K125_D0.9861.00
210_S213_G0.9821.00
189_S192_K0.9791.00
144_S147_E0.9741.00
182_G186_A0.9731.00
103_V224_L0.9721.00
93_V140_A0.9711.00
217_S239_S0.9631.00
65_Q99_M0.9631.00
218_F239_S0.9601.00
137_G203_L0.9551.00
20_C23_T0.9441.00
57_C112_C0.9431.00
53_A108_M0.9421.00
84_L203_L0.9361.00
120_M128_I0.9341.00
215_V243_A0.9311.00
52_I148_M0.9311.00
54_N58_A0.9251.00
201_F205_K0.9231.00
186_A190_R0.9221.00
81_A136_L0.9211.00
74_G97_V0.9191.00
213_G216_G0.9161.00
46_D50_K0.9151.00
87_N207_R0.9140.99
83_A209_I0.9120.99
131_V217_S0.9070.99
117_S121_D0.9060.99
20_C26_I0.9060.99
21_Q24_H0.9010.99
183_A187_K0.8990.99
154_I185_N0.8880.99
85_G95_Y0.8860.99
53_A112_C0.8810.99
79_K83_A0.8770.99
75_K78_A0.8770.99
85_G88_I0.8760.99
158_R162_S0.8740.99
115_V119_L0.8650.99
212_Q215_V0.8610.99
24_H28_T0.8560.99
60_M64_N0.8540.99
22_N25_G0.8480.99
181_E186_A0.8460.99
222_A243_A0.8450.99
82_K86_N0.8420.99
33_S36_L0.8410.99
200_S204_L0.8380.99
107_A114_R0.8380.99
146_A195_E0.8320.99
212_Q251_A0.8300.99
186_A189_S0.8280.99
81_A132_L0.8270.99
238_P241_E0.8240.99
54_N112_C0.8180.99
32_I39_Y0.8170.99
77_L97_V0.8160.99
219_E223_S0.8160.99
201_F249_R0.7980.99
218_F246_I0.7970.99
214_L218_F0.7960.99
51_A55_Q0.7920.99
96_K114_R0.7890.99
182_G233_F0.7820.98
125_D209_I0.7810.98
58_A62_S0.7800.98
121_D127_E0.7770.98
178_D181_E0.7750.98
26_I30_M0.7730.98
80_I128_I0.7730.98
143_H192_K0.7700.98
67_A74_G0.7690.98
227_G230_Q0.7680.98
178_D182_G0.7670.98
187_K191_D0.7650.98
194_S198_D0.7600.98
96_K112_C0.7600.98
41_A153_A0.7590.98
31_A34_S0.7570.98
115_V120_M0.7540.98
159_D163_A0.7520.98
160_A163_A0.7510.98
175_Q178_D0.7470.98
134_H193_E0.7420.98
83_A129_E0.7410.98
91_T139_V0.7400.98
218_F221_L0.7400.98
61_D96_K0.7340.98
67_A117_S0.7320.98
200_S214_L0.7310.98
119_L127_E0.7270.97
66_V96_K0.7230.97
40_K146_A0.7210.97
103_V221_L0.7120.97
71_S74_G0.7060.97
51_A54_N0.7060.97
247_R250_I0.7030.97
140_A199_F0.6980.97
136_L139_V0.6980.97
186_A234_D0.6930.97
40_K149_K0.6900.97
150_A154_I0.6890.97
28_T31_A0.6830.96
52_I55_Q0.6810.96
24_H29_N0.6810.96
212_Q216_G0.6800.96
231_S238_P0.6800.96
101_S117_S0.6790.96
77_L95_Y0.6780.96
103_V122_M0.6780.96
20_C30_M0.6770.96
226_G229_T0.6770.96
67_A99_M0.6730.96
28_T32_I0.6720.96
100_T118_G0.6700.96
22_N26_I0.6690.96
80_I209_I0.6640.96
25_G29_N0.6640.96
160_A164_T0.6610.96
56_G108_M0.6590.96
172_S176_L0.6580.96
113_V132_L0.6570.95
46_D49_A0.6550.95
44_L145_L0.6550.95
23_T26_I0.6540.95
49_A52_I0.6530.95
67_A71_S0.6520.95
156_A159_D0.6490.95
31_A35_G0.6480.95
72_K75_K0.6430.95
135_E193_E0.6420.95
181_E187_K0.6420.95
165_S170_Q0.6410.95
77_L81_A0.6370.95
247_R251_A0.6340.95
77_L80_I0.6340.95
20_C27_D0.6340.95
46_D90_G0.6320.94
175_Q179_I0.6290.94
62_S65_Q0.6290.94
67_A97_V0.6270.94
33_S38_A0.6270.94
177_G181_E0.6230.94
147_E185_N0.6220.94
123_M220_T0.6210.94
20_C24_H0.6180.94
22_N27_D0.6040.93
21_Q29_N0.6010.93
130_G218_F0.5990.93
93_V136_L0.5950.92
113_V136_L0.5940.92
184_I188_Y0.5860.92
81_A125_D0.5860.92
157_A160_A0.5820.92
80_I125_D0.5820.92
159_D162_S0.5820.92
108_M112_C0.5800.91
147_E188_Y0.5790.91
156_A160_A0.5760.91
152_Y156_A0.5750.91
66_V74_G0.5750.91
80_I132_L0.5740.91
201_F250_I0.5720.91
107_A190_R0.5690.91
57_C60_M0.5690.91
136_L140_A0.5630.90
173_R176_L0.5590.90
67_A73_Y0.5580.90
21_Q27_D0.5570.90
38_A42_A0.5570.90
195_E198_D0.5570.90
107_A113_V0.5560.90
53_A109_A0.5550.90
170_Q173_R0.5540.90
77_L120_M0.5520.89
99_M118_G0.5520.89
29_N36_L0.5500.89
157_A164_T0.5500.89
129_E207_R0.5460.89
228_R231_S0.5450.89
27_D41_A0.5450.89
21_Q25_G0.5430.89
204_L210_S0.5420.89
151_S185_N0.5390.88
60_M114_R0.5350.88
60_M106_W0.5340.88
143_H146_A0.5320.88
30_M39_Y0.5310.88
24_H27_D0.5310.88
200_S207_R0.5290.87
182_G185_N0.5280.87
189_S234_D0.5280.87
49_A110_N0.5270.87
46_D142_G0.5270.87
83_A125_D0.5270.87
60_M63_G0.5260.87
25_G30_M0.5260.87
176_L179_I0.5260.87
236_H242_R0.5240.87
155_V158_R0.5230.87
228_R241_E0.5220.87
30_M34_S0.5180.86
86_N89_N0.5180.86
26_I31_A0.5180.86
110_N129_E0.5160.86
20_C25_G0.5150.86
88_I203_L0.5140.86
53_A56_G0.5140.86
25_G32_I0.5140.86
179_I183_A0.5110.85
161_I164_T0.5110.85
128_I132_L0.5110.85
122_M220_T0.5080.85
108_M144_S0.5070.85
70_S74_G0.5050.85
78_A96_K0.5040.85
26_I32_I0.5030.85
156_A163_A0.5030.85
27_D32_I0.5010.84
213_G218_F0.5000.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3c37A 1 0.7874 100 0.252 Contact Map
4aw6A 2 0.9331 100 0.492 Contact Map
4il3A 1 0.9291 100 0.499 Contact Map
3cqbA 4 0.4016 99.7 0.681 Contact Map
4qhjA 2 0.3976 97.4 0.862 Contact Map
3dteA 2 0.6378 96.7 0.876 Contact Map
4jiuA 1 0.3937 96.5 0.878 Contact Map
4jixA 2 0.4213 96.4 0.879 Contact Map
2w3zA 1 0.7283 80.2 0.92 Contact Map
3zukA 2 0.9488 75.2 0.923 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0084 seconds.