GREMLIN Database
GSPF - Putative type II secretion system protein F
UniProt: P41441 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12885
Length: 398 (387)
Sequences: 4837 (3680)
Seq/√Len: 187.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
7_A36_L5.5991.00
65_T126_Y4.8791.00
19_I27_A4.4271.00
202_L206_I4.2341.00
104_A117_H3.8631.00
21_A27_A3.6831.00
289_T302_A3.5021.00
111_L115_L3.2821.00
238_R241_N3.0601.00
27_A41_I2.9101.00
243_H299_Q2.8741.00
203_S381_F2.7991.00
304_A318_L2.7841.00
155_K354_T2.6951.00
64_F98_L2.6051.00
272_S346_V2.6041.00
340_Q343_T2.5671.00
100_Q118_F2.4991.00
190_T212_D2.3471.00
24_E28_R2.3461.00
73_A144_E2.3201.00
68_L81_S2.2891.00
269_R353_E2.2801.00
80_E337_K2.2511.00
202_L205_R2.2361.00
235_W241_N2.2341.00
205_R209_G2.1991.00
157_R161_I2.1861.00
38_L41_I2.1071.00
82_L105_I2.1031.00
236_L245_F2.1011.00
86_G99_N2.0891.00
69_A147_A2.0881.00
6_R16_Q2.0741.00
230_V234_L2.0441.00
104_A109_H2.0431.00
152_N357_Q2.0221.00
116_Q131_K2.0071.00
68_L143_L1.9941.00
6_R14_K1.9931.00
5_Y17_G1.9881.00
176_G180_I1.9691.00
285_M301_L1.9691.00
179_I183_T1.9591.00
6_R40_D1.9451.00
286_N305_A1.9341.00
276_S342_G1.9011.00
100_Q104_A1.8961.00
104_A114_A1.8831.00
246_H295_L1.8771.00
377_L381_F1.8681.00
82_L98_L1.8661.00
180_I184_A1.8581.00
159_K361_A1.8561.00
83_A102_R1.8201.00
213_T221_L1.8181.00
203_S388_Q1.7751.00
172_T176_G1.7631.00
307_N312_N1.7581.00
66_R151_E1.7481.00
261_A355_L1.7351.00
203_S206_I1.7211.00
30_R34_E1.7201.00
199_Q205_R1.7111.00
392_Q395_S1.7051.00
73_A347_R1.7041.00
229_A233_W1.7041.00
387_L391_L1.7031.00
307_N317_S1.7021.00
244_R248_M1.7011.00
126_Y146_L1.6841.00
138_L141_P1.6741.00
271_L284_G1.6631.00
307_N320_Q1.6401.00
233_W237_K1.6331.00
102_R106_L1.6291.00
171_T175_I1.6261.00
60_E91_N1.6181.00
112_S134_E1.6081.00
22_N26_Q1.5921.00
115_L130_V1.5681.00
168_C172_T1.5381.00
135_K283_D1.5341.00
8_M14_K1.5311.00
126_Y150_N1.5281.00
220_T224_T1.5271.00
18_I44_Q1.5231.00
246_H294_N1.4991.00
159_K357_Q1.4931.00
207_L385_S1.4761.00
86_G102_R1.4661.00
26_Q30_R1.4661.00
23_D26_Q1.4601.00
240_N244_R1.4511.00
27_A38_L1.4481.00
212_D215_Q1.4451.00
312_N316_L1.4451.00
246_H249_L1.4231.00
154_Q158_S1.4191.00
228_V232_F1.4121.00
217_T220_T1.4041.00
206_I209_G1.3941.00
69_A144_E1.3891.00
259_I263_N1.3671.00
235_W238_R1.3641.00
283_D286_N1.3591.00
289_T305_A1.3361.00
109_H113_D1.3341.00
269_R350_D1.3301.00
188_K191_E1.3131.00
132_A136_S1.3091.00
261_A359_R1.3081.00
219_P223_A1.3041.00
4_R44_Q1.2891.00
269_R349_A1.2891.00
303_N321_T1.2771.00
267_Y301_L1.2731.00
89_S94_L1.2511.00
268_L328_M1.2491.00
295_L298_R1.2481.00
264_S328_M1.2441.00
243_H296_E1.2441.00
166_Y170_L1.2421.00
101_V115_L1.2261.00
265_A356_Q1.2241.00
73_A140_A1.2231.00
97_V121_L1.2231.00
305_A309_R1.2121.00
178_V378_I1.2081.00
234_L237_K1.2011.00
281_L314_I1.1991.00
152_N353_E1.1981.00
89_S98_L1.1911.00
232_F245_F1.1851.00
201_P204_T1.1821.00
123_D150_N1.1811.00
58_H157_R1.1781.00
242_R295_L1.1761.00
292_L301_L1.1681.00
244_R247_A1.1611.00
170_L375_M1.1561.00
165_I169_M1.1541.00
181_L382_I1.1541.00
263_N297_I1.1531.00
274_L284_G1.1511.00
271_L345_M1.1411.00
300_R321_T1.1341.00
144_E148_D1.1261.00
123_D126_Y1.1231.00
113_D116_Q1.1161.00
226_F230_V1.1151.00
368_E372_I1.1151.00
72_S143_L1.1061.00
265_A353_E1.1061.00
65_T146_L1.1031.00
62_T147_A1.1021.00
271_L281_L1.1011.00
213_T217_T1.1001.00
31_L34_E1.0981.00
303_N306_E1.0971.00
257_P261_A1.0971.00
189_I387_L1.0941.00
212_D216_R1.0941.00
24_E43_P1.0931.00
231_G235_W1.0911.00
225_V229_A1.0891.00
189_I391_L1.0881.00
27_A30_R1.0881.00
116_Q127_R1.0841.00
56_I94_L1.0801.00
380_L384_V1.0691.00
19_I30_R1.0691.00
318_L332_V1.0651.00
243_H295_L1.0641.00
75_A278_G1.0631.00
267_Y271_L1.0541.00
131_K135_K1.0481.00
14_K39_L1.0441.00
78_L111_L1.0431.00
92_K95_G1.0391.00
206_I210_L1.0381.00
183_T214_L1.0371.00
104_A118_F1.0361.00
261_A356_Q1.0361.00
149_Y153_R1.0231.00
97_V122_F1.0121.00
248_M252_V1.0111.00
66_R148_D1.0080.99
97_V118_F1.0040.99
209_G213_T1.0040.99
15_L36_L1.0030.99
285_M308_V0.9970.99
208_L212_D0.9900.99
231_G234_L0.9860.99
68_L78_L0.9840.99
166_Y369_P0.9830.99
58_H154_Q0.9820.99
178_V382_I0.9810.99
301_L304_A0.9770.99
328_M348_A0.9720.99
142_V146_L0.9690.99
182_L210_L0.9690.99
276_S346_V0.9650.99
155_K159_K0.9640.99
235_W244_R0.9580.99
170_L379_V0.9520.99
30_R33_E0.9490.99
227_I231_G0.9480.99
162_Q361_A0.9450.99
190_T194_V0.9440.99
247_A251_R0.9370.99
243_H247_A0.9330.99
158_S354_T0.9330.99
17_G31_L0.9330.99
7_A17_G0.9320.99
59_S158_S0.9310.99
7_A15_L0.9260.99
250_L294_N0.9220.99
389_P392_Q0.9160.99
29_L33_E0.9150.99
70_T344_L0.9120.99
104_A107_E0.9120.99
378_I381_F0.9120.99
319_E333_A0.9090.99
289_T301_L0.9090.99
24_E42_R0.9090.99
155_K357_Q0.9060.99
113_D131_K0.9040.99
204_T385_S0.9020.99
5_Y31_L0.9020.99
119_P123_D0.9020.99
71_L81_S0.8990.99
200_L204_T0.8910.99
390_L394_N0.8900.99
262_I356_Q0.8880.99
148_D152_N0.8860.99
110_P113_D0.8850.99
56_I121_L0.8800.99
372_I375_M0.8800.99
59_S154_Q0.8800.99
267_Y318_L0.8790.99
239_G243_H0.8780.99
130_V143_L0.8770.99
7_A38_L0.8730.99
28_R41_I0.8700.99
354_T358_N0.8670.98
306_E310_Q0.8650.98
346_V350_D0.8650.98
61_L123_D0.8610.98
129_L142_V0.8540.98
180_I185_V0.8520.98
152_N155_K0.8520.98
101_V114_A0.8510.98
116_Q124_S0.8500.98
313_S316_L0.8470.98
124_S290_E0.8420.98
315_H336_E0.8410.98
83_A106_L0.8400.98
179_I214_L0.8380.98
302_A305_A0.8380.98
230_V233_W0.8320.98
145_K276_S0.8310.98
335_G339_G0.8290.98
133_G142_V0.8290.98
280_P284_G0.8280.98
170_L174_A0.8270.98
249_L259_I0.8260.98
292_L297_I0.8240.98
67_Q331_M0.8210.98
10_Q35_G0.8190.98
300_R323_I0.8180.98
94_L97_V0.8180.98
58_H62_T0.8170.98
79_E102_R0.8170.98
131_K290_E0.8170.98
27_A31_L0.8150.98
356_Q360_I0.8130.98
363_T367_F0.8110.98
308_V314_I0.8060.98
135_K287_L0.8030.97
298_R302_A0.8020.97
224_T228_V0.8020.97
383_V391_L0.7980.97
272_S349_A0.7980.97
171_T371_L0.7980.97
182_L186_V0.7970.97
103_S107_E0.7970.97
242_R246_H0.7940.97
24_E27_A0.7940.97
134_E339_G0.7870.97
173_V177_V0.7840.97
165_I365_S0.7840.97
232_F236_L0.7830.97
4_R16_Q0.7810.97
109_H117_H0.7790.97
262_I266_R0.7790.97
304_A317_S0.7780.97
62_T150_N0.7780.97
161_I165_I0.7760.97
132_A270_T0.7750.97
160_L367_F0.7730.97
162_Q362_L0.7700.97
315_H333_A0.7670.97
252_V255_I0.7650.97
289_T298_R0.7630.97
165_I168_C0.7590.96
119_P122_F0.7570.96
331_M348_A0.7560.96
83_A87_Q0.7550.96
218_G222_L0.7520.96
65_T143_L0.7510.96
65_T150_N0.7500.96
306_E309_R0.7500.96
66_R147_A0.7500.96
247_A296_E0.7490.96
190_T208_L0.7470.96
173_V373_I0.7450.96
109_H114_A0.7400.96
70_T338_S0.7390.96
189_I193_F0.7350.96
127_R131_K0.7310.96
207_L381_F0.7310.96
199_Q203_S0.7310.96
252_V256_G0.7270.96
165_I362_L0.7250.95
256_G260_C0.7240.95
4_R18_I0.7240.95
307_N310_Q0.7220.95
61_L65_T0.7220.95
149_Y270_T0.7190.95
347_R350_D0.7190.95
141_P276_S0.7180.95
98_L101_V0.7180.95
142_V273_I0.7180.95
270_T291_S0.7160.95
7_A39_L0.7130.95
234_L238_R0.7120.95
222_L226_F0.7090.95
186_V208_L0.7090.95
233_W236_L0.7080.95
23_D29_L0.7080.95
265_A349_A0.7050.95
257_P359_R0.7050.95
86_G98_L0.7040.95
296_E300_R0.7020.95
248_M251_R0.7020.95
62_T154_Q0.7000.95
6_R39_L0.6980.94
112_S131_K0.6970.94
264_S348_A0.6960.94
204_T389_P0.6950.94
362_L365_S0.6890.94
87_Q319_E0.6880.94
119_P127_R0.6820.94
223_A227_I0.6810.94
325_P352_Q0.6810.94
268_L356_Q0.6810.94
336_E339_G0.6800.94
387_L390_L0.6790.94
216_R219_P0.6780.94
169_M366_I0.6750.93
158_S358_N0.6720.93
366_I370_A0.6720.93
184_A188_K0.6710.93
60_E94_L0.6710.93
64_F94_L0.6700.93
5_Y27_A0.6680.93
100_Q117_H0.6670.93
67_Q327_M0.6660.93
62_T69_A0.6650.93
14_K38_L0.6640.93
304_A332_V0.6610.93
77_P80_E0.6600.93
108_G311_G0.6600.93
130_V133_G0.6590.93
15_L34_E0.6590.93
239_G295_L0.6580.93
300_R303_N0.6580.93
189_I192_Q0.6550.92
95_G99_N0.6530.92
25_R32_R0.6520.92
193_F393_L0.6470.92
325_P328_M0.6460.92
58_H150_N0.6450.92
263_N293_N0.6420.92
300_R324_F0.6420.92
332_V341_L0.6420.92
381_F388_Q0.6400.91
312_N320_Q0.6400.91
303_N307_N0.6400.91
203_S385_S0.6390.91
387_L394_N0.6390.91
357_Q361_A0.6380.91
314_I318_L0.6380.91
371_L374_T0.6370.91
101_V118_F0.6370.91
56_I93_R0.6360.91
36_L39_L0.6340.91
296_E299_Q0.6330.91
115_L126_Y0.6320.91
264_S268_L0.6320.91
85_I98_L0.6320.91
207_L375_M0.6280.91
185_V189_I0.6280.91
145_K346_V0.6270.91
5_Y19_I0.6270.91
19_I34_E0.6260.91
53_R120_T0.6250.90
184_A376_A0.6250.90
203_S207_L0.6240.90
318_L329_L0.6240.90
26_Q33_E0.6230.90
380_L383_V0.6230.90
111_L143_L0.6230.90
269_R272_S0.6230.90
220_T223_A0.6210.90
8_M39_L0.6210.90
174_A391_L0.6190.90
182_L211_S0.6180.90
338_S341_L0.6180.90
134_E137_G0.6170.90
140_A359_R0.6170.90
275_Q284_G0.6160.90
107_E310_Q0.6160.90
304_A308_V0.6150.90
210_L213_T0.6140.90
181_L386_V0.6130.90
344_L372_I0.6120.90
221_L225_V0.6110.89
241_N245_F0.6110.89
122_F130_V0.6100.89
331_M352_Q0.6080.89
81_S85_I0.6080.89
316_L319_E0.6070.89
280_P283_D0.6060.89
370_A373_I0.6050.89
140_A342_G0.6040.89
223_A240_N0.6040.89
374_T378_I0.6040.89
20_D26_Q0.6030.89
186_V198_Q0.6020.89
316_L320_Q0.6020.89
23_D27_A0.6020.89
191_E194_V0.6000.89
264_S352_Q0.5990.89
205_R208_L0.5980.88
97_V101_V0.5960.88
83_A86_G0.5960.88
322_A329_L0.5950.88
327_M331_M0.5940.88
164_L371_L0.5940.88
6_R15_L0.5910.88
100_Q103_S0.5900.88
137_G339_G0.5900.88
369_P376_A0.5890.88
82_L102_R0.5890.88
53_R93_R0.5890.88
201_P205_R0.5880.88
275_Q342_G0.5880.88
10_Q44_Q0.5870.88
367_F371_L0.5860.87
180_I183_T0.5850.87
290_E298_R0.5830.87
365_S368_E0.5830.87
10_Q37_F0.5830.87
206_I381_F0.5830.87
34_E38_L0.5820.87
130_V139_L0.5820.87
181_L185_V0.5810.87
87_Q326_P0.5810.87
240_N243_H0.5800.87
40_D43_P0.5800.87
274_L279_V0.5800.87
69_A140_A0.5790.87
54_P93_R0.5790.87
228_V234_L0.5780.87
268_L348_A0.5780.87
369_P389_P0.5770.87
64_F68_L0.5770.87
193_F200_L0.5760.87
209_G212_D0.5750.86
84_V334_S0.5740.86
57_S60_E0.5730.86
376_A394_N0.5730.86
73_A343_T0.5720.86
267_Y292_L0.5720.86
160_L164_L0.5720.86
42_R46_S0.5710.86
151_E347_R0.5710.86
312_N317_S0.5700.86
147_A151_E0.5680.86
274_L287_L0.5670.86
116_Q119_P0.5650.86
22_N25_R0.5620.85
5_Y15_L0.5620.85
4_R45_K0.5600.85
272_S275_Q0.5600.85
56_I97_V0.5590.85
198_Q393_L0.5590.85
84_V337_K0.5560.85
226_F233_W0.5540.84
359_R362_L0.5540.84
253_A256_G0.5540.84
289_T292_L0.5530.84
124_S298_R0.5500.84
182_L214_L0.5440.83
177_V181_L0.5430.83
177_V391_L0.5420.83
264_S325_P0.5420.83
57_S91_N0.5410.83
213_T216_R0.5390.83
15_L27_A0.5390.83
380_L385_S0.5390.83
260_C264_S0.5390.83
177_V382_I0.5370.83
344_L347_R0.5350.82
166_Y368_E0.5330.82
38_L42_R0.5330.82
66_R355_L0.5330.82
186_V211_S0.5330.82
129_L133_G0.5310.82
124_S293_N0.5310.82
31_L36_L0.5300.82
74_A344_L0.5290.82
136_S142_V0.5290.82
174_A196_M0.5290.82
232_F241_N0.5290.82
277_S291_S0.5280.81
149_Y181_L0.5270.81
54_P91_N0.5260.81
123_D153_R0.5240.81
295_L299_Q0.5240.81
257_P260_C0.5240.81
368_E389_P0.5230.81
268_L345_M0.5220.81
93_R96_D0.5220.81
281_L341_L0.5210.81
255_I259_I0.5200.81
66_R69_A0.5200.81
370_A374_T0.5200.81
4_R8_M0.5200.81
29_L42_R0.5200.81
335_G341_L0.5190.80
175_I179_I0.5180.80
386_V391_L0.5180.80
133_G136_S0.5180.80
189_I386_V0.5180.80
245_F249_L0.5180.80
368_E379_V0.5160.80
122_F127_R0.5150.80
391_L394_N0.5150.80
15_L35_G0.5150.80
8_M37_F0.5150.80
246_H250_L0.5150.80
260_C297_I0.5140.80
99_N103_S0.5140.80
97_V115_L0.5120.79
220_T227_I0.5110.79
15_L18_I0.5110.79
105_I111_L0.5100.79
334_S380_L0.5100.79
129_L372_I0.5090.79
105_I114_A0.5090.79
67_Q88_Q0.5090.79
302_A306_E0.5080.79
319_E329_L0.5080.79
149_Y266_R0.5060.79
300_R329_L0.5060.79
13_Q18_I0.5040.78
211_S215_Q0.5040.78
148_D346_V0.5040.78
73_A286_N0.5030.78
19_I31_L0.5030.78
71_L76_L0.5030.78
23_D33_E0.5020.78
181_L188_K0.5020.78
4_R42_R0.5020.78
8_M42_R0.5010.78
196_M393_L0.5010.78
153_R393_L0.5000.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3c1qA 2 0.304 99.9 0.73 Contact Map
4hhxA 1 0.2538 99.9 0.731 Contact Map
2whnA 1 0.2764 99.8 0.759 Contact Map
4ev6A 3 0.4648 25.3 0.96 Contact Map
2vldA 2 0.1106 25 0.96 Contact Map
4i0uA 4 0.4724 24 0.961 Contact Map
2m8rA 1 0.2563 22.7 0.961 Contact Map
4oi3A 2 0.1206 21.7 0.962 Contact Map
1c07A 1 0.2111 15.6 0.964 Contact Map
2wswA 3 0.5427 15.1 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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