YJGX - ECOLI - GREMLIN Contacts

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YJGX - Putative phosphoethanolamine transferase YjgX

UniProt:	P39349	Sequences: MNRPVFPVYHFLVSAAILVFVVIFWRTHHRDHRNWLALRLFVLCSVNRWPLRMVKGTVVGTTDTLREMQRYEQLSSTGLTTGKSSRXAPLYDTIVIVTGESVRRDYMSVYDYPVPTTPWLNTAPGLFIDDYTSTASSTVSSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISNQGKLGEHDTRISVIASDAEHATFLKKGSFASRKTDDKLLLQETERALADTSSPKIIFLHMMGSHPNPCDSLNSXPNNYLEQYPRKIACYLASISKLDNFLGQLDGILRRYSRHFAMLYFSGLGLSVSDSANPVHHYGHVQGGYSVPLIITASDITSHQPVSRKISARHFAGIFQWMTGICTENIPPFNPLTDEDN	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG12543
Length:	371 (318)
Sequences:	6040 (4767)
Seq/√Len:	267.3

ECOLI - YJGX OPGE EPTC EPTA YHBX EPTB YEJM YIDJ YDEN ASLA OPGB
BACSU - LTAS2 YQGS LTAS1 YVGJ
HALSA - Q9HQ44 Q9HSV3 Q9HQQ2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
351_W	365_P	5.794	1.00
119_W	277_G	4.847	1.00
129_D	337_S	4.226	1.00
224_A	286_Y	3.520	1.00
131_Y	340_I	3.328	1.00
119_W	122_T	3.232	1.00
220_E	282_I	3.183	1.00
99_G	102_V	2.947	1.00
216_L	236_M	2.917	1.00
281_G	285_R	2.872	1.00
170_S	228_S	2.852	1.00
280_D	292_M	2.772	1.00
94_I	164_A	2.706	1.00
95_V	292_M	2.699	1.00
340_I	366_L	2.644	1.00
218_E	221_R	2.564	1.00
277_G	281_G	2.421	1.00
92_D	289_H	2.373	1.00
94_I	353_T	2.371	1.00
115_P	121_N	2.255	1.00
117_T	121_N	2.219	1.00
108_S	121_N	2.209	1.00
97_V	294_Y	2.196	1.00
348_I	367_T	2.189	1.00
108_S	117_T	2.135	1.00
224_A	282_I	2.094	1.00
291_A	325_I	2.089	1.00
281_G	284_R	2.041	1.00
251_P	274_N	2.039	1.00
97_V	276_L	2.020	1.00
93_T	290_F	2.016	1.00
90_L	289_H	2.016	1.00
110_Y	127_F	1.948	1.00
96_I	293_L	1.895	1.00
93_T	230_K	1.881	1.00
282_I	286_Y	1.873	1.00
217_Q	278_Q	1.838	1.00
164_A	353_T	1.823	1.00
221_R	225_D	1.775	1.00
216_L	220_E	1.765	1.00
234_L	279_L	1.762	1.00
248_N	271_K	1.743	1.00
327_A	330_I	1.735	1.00
283_L	292_M	1.675	1.00
253_N	270_S	1.657	1.00
186_R	191_A	1.652	1.00
282_I	285_R	1.625	1.00
95_V	230_K	1.616	1.00
213_K	278_Q	1.604	1.00
172_W	222_A	1.591	1.00
95_V	290_F	1.576	1.00
215_L	234_L	1.575	1.00
309_V	312_Y	1.563	1.00
183_H	186_R	1.539	1.00
102_V	269_I	1.531	1.00
126_L	334_Q	1.527	1.00
249_S	252_N	1.516	1.00
219_T	232_I	1.509	1.00
128_I	323_L	1.506	1.00
96_I	231_I	1.505	1.00
158_N	166_K	1.499	1.00
275_F	278_Q	1.496	1.00
293_L	349_F	1.484	1.00
293_L	325_I	1.475	1.00
316_Q	320_S	1.475	1.00
311_H	314_H	1.474	1.00
99_G	272_L	1.469	1.00
254_Y	270_S	1.446	1.00
163_L	350_Q	1.441	1.00
213_K	271_K	1.425	1.00
188_S	191_A	1.415	1.00
102_V	107_M	1.410	1.00
92_D	291_A	1.406	1.00
240_H	297_G	1.392	1.00
239_S	269_I	1.386	1.00
212_D	238_G	1.385	1.00
99_G	294_Y	1.382	1.00
131_Y	323_L	1.381	1.00
270_S	274_N	1.375	1.00
104_R	320_S	1.374	1.00
350_Q	355_I	1.364	1.00
174_I	232_I	1.363	1.00
148_Y	151_E	1.361	1.00
94_I	291_A	1.348	1.00
222_A	225_D	1.347	1.00
117_T	120_L	1.338	1.00
189_V	192_S	1.336	1.00
236_M	275_F	1.323	1.00
347_G	363_F	1.315	1.00
216_L	279_L	1.304	1.00
102_V	294_Y	1.300	1.00
346_A	361_P	1.296	1.00
129_D	335_P	1.295	1.00
138_T	235_H	1.294	1.00
138_T	142_L	1.291	1.00
126_L	336_V	1.288	1.00
220_E	224_A	1.283	1.00
219_T	234_L	1.270	1.00
102_V	272_L	1.269	1.00
108_S	115_P	1.261	1.00
195_E	198_T	1.249	1.00
134_T	144_R	1.232	1.00
327_A	331_T	1.221	1.00
220_E	286_Y	1.219	1.00
49_W	53_M	1.217	1.00
310_H	313_G	1.210	1.00
96_I	295_F	1.209	1.00
49_W	52_R	1.201	1.00
213_K	275_F	1.197	1.00
174_I	222_A	1.193	1.00
247_L	252_N	1.190	1.00
278_Q	281_G	1.176	1.00
173_W	177_Q	1.174	1.00
127_F	335_P	1.166	1.00
147_I	159_N	1.166	1.00
119_W	281_G	1.166	1.00
112_Y	266_L	1.163	1.00
75_S	78_G	1.162	1.00
183_H	187_I	1.161	1.00
145_T	295_F	1.157	1.00
91_Y	167_A	1.157	1.00
310_H	315_V	1.151	1.00
187_I	191_A	1.148	1.00
118_P	273_D	1.145	1.00
274_N	278_Q	1.141	1.00
48_R	51_L	1.128	1.00
48_R	56_G	1.122	1.00
70_R	73_Q	1.117	1.00
98_T	295_F	1.110	1.00
345_F	349_F	1.108	1.00
133_S	321_V	1.092	1.00
173_W	233_F	1.084	1.00
164_A	350_Q	1.076	1.00
279_L	292_M	1.071	1.00
93_T	288_R	1.069	1.00
47_N	50_P	1.068	1.00
106_Y	266_L	1.066	1.00
47_N	52_R	1.066	1.00
160_V	231_I	1.065	1.00
280_D	284_R	1.059	1.00
187_I	192_S	1.052	1.00
328_S	331_T	1.052	1.00
248_N	251_P	1.051	1.00
212_D	216_L	1.047	1.00
73_Q	76_S	1.046	1.00
48_R	52_R	1.046	1.00
48_R	53_M	1.041	1.00
164_A	231_I	1.041	1.00
311_H	315_V	1.040	1.00
256_E	260_R	1.037	1.00
46_V	50_P	1.036	1.00
138_T	143_S	1.034	1.00
149_D	157_G	1.034	1.00
295_F	342_A	1.029	1.00
291_A	327_A	1.023	1.00
278_Q	282_I	1.021	1.00
122_T	284_R	1.020	1.00
50_P	53_M	1.020	1.00
149_D	152_Q	1.011	1.00
216_L	275_F	1.011	1.00
148_Y	347_G	1.004	1.00
239_S	268_S	1.003	1.00
52_R	56_G	1.003	1.00
138_T	240_H	0.994	1.00
141_S	342_A	0.992	1.00
106_Y	269_I	0.991	1.00
173_W	235_H	0.990	1.00
109_V	127_F	0.979	1.00
310_H	314_H	0.974	1.00
309_V	313_G	0.974	1.00
119_W	273_D	0.970	1.00
66_R	70_R	0.970	1.00
167_A	355_I	0.964	1.00
52_R	55_K	0.960	1.00
323_L	345_F	0.959	1.00
59_V	63_D	0.955	1.00
67_E	70_R	0.954	1.00
220_E	278_Q	0.951	1.00
47_N	56_G	0.941	1.00
223_L	232_I	0.941	1.00
274_N	277_G	0.938	1.00
66_R	69_Q	0.937	1.00
95_V	279_L	0.936	1.00
172_W	232_I	0.934	1.00
47_N	51_L	0.928	1.00
243_P	300_L	0.925	1.00
188_S	192_S	0.922	1.00
98_T	142_L	0.908	1.00
145_T	345_F	0.906	1.00
296_S	321_V	0.906	1.00
184_D	187_I	0.906	1.00
68_M	72_E	0.904	1.00
130_D	320_S	0.903	1.00
122_T	277_G	0.903	1.00
212_D	215_L	0.903	1.00
185_T	188_S	0.903	1.00
254_Y	267_A	0.903	1.00
195_E	200_L	0.899	1.00
346_A	350_Q	0.899	1.00
150_Y	343_R	0.894	1.00
305_S	308_P	0.892	1.00
172_W	230_K	0.885	1.00
178_G	181_G	0.883	1.00
55_K	58_V	0.881	1.00
114_V	266_L	0.881	1.00
291_A	352_M	0.880	1.00
49_W	55_K	0.873	1.00
250_X	253_N	0.872	1.00
54_V	58_V	0.871	1.00
102_V	239_S	0.867	1.00
47_N	54_V	0.864	1.00
163_L	166_K	0.863	1.00
244_C	264_C	0.858	1.00
65_L	74_L	0.857	1.00
169_Y	229_P	0.855	1.00
69_Q	72_E	0.853	1.00
221_R	224_A	0.849	1.00
247_L	267_A	0.846	1.00
283_L	287_S	0.842	1.00
212_D	271_K	0.838	0.99
72_E	76_S	0.833	0.99
312_Y	315_V	0.832	0.99
276_L	280_D	0.827	0.99
214_L	217_Q	0.827	0.99
249_S	253_N	0.827	0.99
97_V	279_L	0.825	0.99
122_T	281_G	0.823	0.99
94_I	169_Y	0.821	0.99
101_S	298_L	0.821	0.99
119_W	274_N	0.821	0.99
96_I	233_F	0.820	0.99
49_W	56_G	0.817	0.99
284_R	326_T	0.814	0.99
247_L	251_P	0.814	0.99
46_V	49_W	0.812	0.99
118_P	121_N	0.809	0.99
46_V	51_L	0.809	0.99
140_S	143_S	0.807	0.99
72_E	75_S	0.806	0.99
97_V	292_M	0.806	0.99
316_Q	319_Y	0.798	0.99
51_L	55_K	0.797	0.99
130_D	339_K	0.797	0.99
48_R	57_T	0.797	0.99
218_E	222_A	0.794	0.99
138_T	145_T	0.789	0.99
47_N	57_T	0.788	0.99
275_F	279_L	0.784	0.99
73_Q	79_L	0.784	0.99
252_N	264_C	0.780	0.99
196_H	200_L	0.775	0.99
50_P	55_K	0.775	0.99
115_P	270_S	0.774	0.99
295_F	345_F	0.769	0.99
163_L	346_A	0.768	0.99
251_P	271_K	0.768	0.99
119_W	280_D	0.765	0.99
174_I	219_T	0.764	0.99
74_L	78_G	0.763	0.99
87_X	90_L	0.761	0.99
49_W	57_T	0.761	0.99
56_G	59_V	0.759	0.99
138_T	297_G	0.759	0.99
100_E	239_S	0.757	0.99
236_M	272_L	0.756	0.99
98_T	235_H	0.755	0.99
219_T	223_L	0.752	0.99
168_G	229_P	0.751	0.99
94_I	231_I	0.751	0.99
247_L	254_Y	0.746	0.99
223_L	283_L	0.745	0.99
147_I	150_Y	0.744	0.99
73_Q	77_T	0.744	0.99
43_L	46_V	0.744	0.99
95_V	232_I	0.743	0.99
280_D	326_T	0.739	0.99
186_R	190_I	0.739	0.99
178_G	184_D	0.738	0.99
50_P	57_T	0.734	0.99
58_V	62_T	0.734	0.99
180_L	184_D	0.733	0.99
271_K	275_F	0.733	0.99
276_L	294_Y	0.732	0.99
288_R	329_D	0.731	0.99
280_D	294_Y	0.731	0.99
66_R	73_Q	0.731	0.99
123_A	277_G	0.731	0.99
132_T	320_S	0.731	0.99
288_R	328_S	0.730	0.99
42_V	45_S	0.729	0.99
352_M	367_T	0.728	0.99
69_Q	75_S	0.726	0.99
186_R	192_S	0.725	0.99
134_T	341_S	0.725	0.99
291_A	349_F	0.723	0.98
58_V	61_T	0.720	0.98
238_G	271_K	0.718	0.98
65_L	71_Y	0.714	0.98
68_M	71_Y	0.714	0.98
216_L	278_Q	0.713	0.98
330_I	333_H	0.710	0.98
178_G	182_E	0.709	0.98
160_V	349_F	0.707	0.98
252_N	255_L	0.705	0.98
147_I	151_E	0.705	0.98
70_R	74_L	0.700	0.98
197_A	200_L	0.697	0.98
74_L	77_T	0.695	0.98
94_I	349_F	0.692	0.98
98_T	145_T	0.692	0.98
126_L	331_T	0.690	0.98
93_T	229_P	0.690	0.98
85_S	367_T	0.686	0.98
116_T	270_S	0.686	0.98
67_E	74_L	0.686	0.98
51_L	59_V	0.684	0.98
216_L	272_L	0.683	0.98
51_L	54_V	0.683	0.98
217_Q	220_E	0.683	0.98
52_R	59_V	0.681	0.98
209_K	237_M	0.677	0.98
77_T	80_T	0.674	0.98
123_A	284_R	0.673	0.98
92_D	229_P	0.672	0.98
65_L	68_M	0.669	0.98
211_D	215_L	0.667	0.97
47_N	55_K	0.665	0.97
116_T	120_L	0.661	0.97
98_T	233_F	0.661	0.97
55_K	59_V	0.660	0.97
185_T	189_V	0.659	0.97
96_I	145_T	0.657	0.97
172_W	175_S	0.657	0.97
238_G	268_S	0.653	0.97
148_Y	152_Q	0.653	0.97
169_Y	353_T	0.648	0.97
321_V	342_A	0.646	0.97
210_T	271_K	0.645	0.97
214_L	218_E	0.645	0.97
53_M	56_G	0.644	0.97
309_V	315_V	0.641	0.97
103_R	302_V	0.640	0.97
45_S	55_K	0.640	0.97
265_Y	269_I	0.639	0.97
95_V	283_L	0.639	0.97
67_E	71_Y	0.638	0.97
43_L	47_N	0.635	0.97
132_T	319_Y	0.634	0.97
96_I	345_F	0.632	0.96
105_D	317_G	0.631	0.96
75_S	79_L	0.630	0.96
263_A	267_A	0.630	0.96
196_H	199_F	0.630	0.96
138_T	144_R	0.629	0.96
76_S	79_L	0.626	0.96
164_A	355_I	0.626	0.96
99_G	276_L	0.626	0.96
305_S	317_G	0.624	0.96
65_L	72_E	0.623	0.96
64_T	68_M	0.622	0.96
217_Q	282_I	0.622	0.96
91_Y	229_P	0.622	0.96
48_R	55_K	0.619	0.96
222_A	230_K	0.619	0.96
103_R	299_G	0.618	0.96
123_A	280_D	0.615	0.96
294_Y	324_I	0.614	0.96
57_T	62_T	0.612	0.96
53_M	58_V	0.609	0.96
70_R	76_S	0.606	0.96
45_S	49_W	0.605	0.96
300_L	318_G	0.604	0.95
66_R	71_Y	0.601	0.95
197_A	201_K	0.600	0.95
272_L	275_F	0.600	0.95
46_V	55_K	0.600	0.95
252_N	267_A	0.599	0.95
52_R	62_T	0.599	0.95
100_E	240_H	0.599	0.95
344_H	363_F	0.595	0.95
102_V	120_L	0.594	0.95
178_G	195_E	0.592	0.95
323_L	340_I	0.592	0.95
46_V	53_M	0.591	0.95
50_P	56_G	0.589	0.95
73_Q	78_G	0.587	0.95
322_P	325_I	0.586	0.95
116_T	266_L	0.585	0.95
60_G	63_D	0.585	0.95
51_L	58_V	0.584	0.95
271_K	274_N	0.582	0.94
45_S	52_R	0.581	0.94
100_E	297_G	0.581	0.94
46_V	52_R	0.580	0.94
173_W	187_I	0.580	0.94
212_D	236_M	0.578	0.94
301_S	318_G	0.578	0.94
64_T	71_Y	0.577	0.94
309_V	314_H	0.576	0.94
185_T	191_A	0.576	0.94
120_L	276_L	0.575	0.94
45_S	48_R	0.574	0.94
51_L	56_G	0.573	0.94
83_K	86_R	0.571	0.94
331_T	334_Q	0.569	0.94
45_S	53_M	0.568	0.94
52_R	60_G	0.567	0.94
223_L	279_L	0.565	0.94
147_I	152_Q	0.562	0.93
253_N	256_E	0.561	0.93
109_V	121_N	0.560	0.93
161_V	171_T	0.560	0.93
317_G	320_S	0.559	0.93
165_A	171_T	0.558	0.93
193_D	199_F	0.556	0.93
230_K	290_F	0.554	0.93
53_M	57_T	0.554	0.93
138_T	298_L	0.554	0.93
43_L	58_V	0.553	0.93
45_S	58_V	0.552	0.93
239_S	272_L	0.551	0.93
267_A	270_S	0.548	0.93
93_T	289_H	0.546	0.92
94_I	293_L	0.545	0.92
279_L	282_I	0.544	0.92
61_T	65_L	0.542	0.92
347_G	361_P	0.542	0.92
193_D	198_T	0.541	0.92
106_Y	265_Y	0.541	0.92
43_L	48_R	0.538	0.92
64_T	67_E	0.536	0.92
71_Y	77_T	0.535	0.92
104_R	110_Y	0.533	0.92
137_S	140_S	0.532	0.91
70_R	79_L	0.532	0.91
344_H	348_I	0.531	0.91
254_Y	263_A	0.530	0.91
70_R	77_T	0.530	0.91
45_S	50_P	0.530	0.91
115_P	118_P	0.530	0.91
49_W	58_V	0.530	0.91
122_T	125_G	0.530	0.91
69_Q	73_Q	0.529	0.91
108_S	116_T	0.527	0.91
184_D	188_S	0.524	0.91
144_R	343_R	0.524	0.91
140_S	144_R	0.523	0.91
177_Q	235_H	0.523	0.91
101_S	300_L	0.522	0.91
122_T	280_D	0.521	0.91
52_R	57_T	0.519	0.90
56_G	61_T	0.518	0.90
217_Q	221_R	0.518	0.90
66_R	78_G	0.515	0.90
103_R	304_D	0.515	0.90
160_V	346_A	0.515	0.90
99_G	239_S	0.514	0.90
59_V	62_T	0.514	0.90
53_M	63_D	0.513	0.90
46_V	60_G	0.513	0.90
163_L	355_I	0.512	0.90
88_A	91_Y	0.511	0.90
120_L	324_I	0.511	0.90
99_G	216_L	0.510	0.90
54_V	61_T	0.510	0.90
47_N	53_M	0.510	0.90
183_H	190_I	0.508	0.89
46_V	54_V	0.508	0.89
362_P	365_P	0.507	0.89
102_V	117_T	0.506	0.89
133_S	141_S	0.505	0.89
54_V	57_T	0.504	0.89
92_D	327_A	0.504	0.89
48_R	59_V	0.504	0.89
165_A	191_A	0.501	0.89
42_V	65_L	0.501	0.89
209_K	214_L	0.500	0.89

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4uorA	3	0.8113	100	0.387	Contact Map
2w5qA	1	0.8221	100	0.389	Contact Map
4kavA	1	0.8032	100	0.395	Contact Map
3lxqA	1	0.8113	100	0.401	Contact Map
2w8dA	1	0.8248	100	0.403	Contact Map
4upkA	3	0.7493	100	0.404	Contact Map
4uopA	2	0.8194	100	0.405	Contact Map
4tn0A	1	0.7547	100	0.408	Contact Map
2vqrA	3	0.7466	100	0.411	Contact Map
4uplA	2	0.7547	100	0.411	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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