GREMLIN Database
MSCM - Miniconductance mechanosensitive channel MscM
UniProt: P39285 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12478
Length: 1107 (1042)
Sequences: 436 (306)
Seq/√Len: 9.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
1006_V1010_L6.3801.00
917_E972_K5.3751.00
1012_T1016_R5.3521.00
945_L967_I5.2791.00
911_L915_L5.2291.00
999_A1036_G4.5510.99
997_A1006_V4.3270.99
996_P999_A4.2730.99
999_A1074_M4.0990.99
911_L919_F4.0790.99
28_I52_A3.8690.98
1005_E1008_E3.7860.98
196_S200_R3.7580.98
1014_A1042_L3.5430.97
959_I969_V3.4730.96
1033_L1038_Q3.3990.96
1006_V1040_F3.3960.96
996_P1036_G3.3630.95
110_I222_L3.3450.95
946_T962_W3.3320.95
1001_A1030_L3.2640.95
777_Q853_A3.2040.94
1030_L1078_P3.1540.94
1000_D1038_Q3.1450.93
1036_G1074_M3.1340.93
852_P860_L3.0580.92
1000_D1064_L3.0170.92
830_T833_A2.9720.91
979_F986_D2.8930.90
995_I1074_M2.8390.89
1034_Q1038_Q2.8330.89
496_F524_W2.8080.89
530_S893_I2.8030.89
811_I888_V2.7950.88
60_K811_I2.7810.88
996_P1076_F2.7460.87
989_T1046_A2.7400.87
704_S798_W2.6410.85
911_L923_I2.6070.84
999_A1030_L2.6030.84
28_I33_E2.5940.84
996_P1078_P2.5920.84
905_A920_A2.5780.84
996_P1074_M2.5660.83
918_I924_S2.5450.83
722_M943_R2.5410.83
996_P1030_L2.5310.83
777_Q850_N2.5160.82
271_A346_Q2.4970.82
997_A1010_L2.4950.82
1033_L1074_M2.4820.81
525_S904_V2.4430.80
917_E920_A2.4270.80
1004_E1065_A2.4070.79
1030_L1036_G2.4040.79
1005_E1070_H2.3950.79
968_I991_V2.3180.76
961_D965_K2.3140.76
1035_Q1039_I2.3130.76
897_W902_W2.3120.76
1012_T1066_G2.3120.76
1030_L1074_M2.3080.76
1062_L1065_A2.3050.76
945_L983_S2.3020.76
427_R716_H2.2720.75
32_L46_V2.2700.75
973_A977_E2.2670.75
138_L141_L2.2640.75
939_T962_W2.2530.74
1038_Q1074_M2.2140.73
1000_D1033_L2.1880.72
237_A241_L2.1870.72
621_D624_E2.1760.72
950_T960_S2.1710.71
1000_D1074_M2.1620.71
1038_Q1060_H2.1600.71
996_P1037_I2.1470.70
944_D1045_Y2.1370.70
1001_A1034_Q2.1360.70
510_V731_F2.1170.69
911_L961_D2.1020.69
897_W901_Q2.1010.69
986_D991_V2.0940.68
881_L932_K2.0920.68
1013_A1066_G2.0920.68
998_P1039_I2.0920.68
1030_L1033_L2.0840.68
1033_L1082_R2.0840.68
518_T585_H2.0760.68
999_A1033_L2.0650.67
958_T983_S2.0630.67
932_K999_A2.0610.67
903_L920_A2.0510.67
191_E195_L2.0490.67
993_L998_P2.0470.67
905_A972_K2.0450.67
962_W1060_H2.0390.66
860_L872_A2.0220.66
999_A1078_P2.0030.65
928_I934_I1.9980.65
622_D625_F1.9960.65
1017_C1058_E1.9920.64
151_Q179_D1.9910.64
193_A770_D1.9800.64
513_D720_R1.9750.64
999_A1039_I1.9730.64
585_H856_E1.9670.63
999_A1076_F1.9660.63
1036_G1073_D1.9650.63
1073_D1083_L1.9610.63
642_A995_I1.9440.62
430_F847_L1.9280.62
893_I908_G1.9160.61
66_I69_Y1.9120.61
1033_L1078_P1.9120.61
1031_V1041_E1.9080.61
496_F992_V1.9040.61
1060_H1074_M1.9030.61
1006_V1080_Q1.9020.61
920_A972_K1.9020.61
909_V912_G1.8830.60
518_T693_T1.8760.60
1009_I1067_F1.8680.59
1014_A1020_V1.8670.59
774_I968_I1.8550.59
1001_A1033_L1.8540.59
996_P1001_A1.8460.58
1001_A1078_P1.8360.58
809_E997_A1.8360.58
857_L953_N1.8340.58
820_V823_V1.8280.57
69_Y200_R1.8270.57
1010_L1060_H1.8190.57
430_F779_L1.8090.57
915_L926_L1.8080.57
961_D989_T1.8050.56
33_E36_K1.7930.56
946_T1031_V1.7930.56
1078_P1082_R1.7830.56
993_L1043_R1.7830.56
998_P1073_D1.7750.55
904_V920_A1.7640.55
876_I880_L1.7550.54
513_D585_H1.7510.54
513_D989_T1.7430.54
905_A915_L1.7430.54
961_D1041_E1.7330.53
961_D1006_V1.7290.53
996_P1034_Q1.7110.52
851_L860_L1.7050.52
796_V876_I1.7050.52
724_I932_K1.7030.52
1083_L1086_L1.7010.52
917_E976_T1.6860.51
383_L667_T1.6830.51
1033_L1060_H1.6700.50
903_L972_K1.6580.50
733_R1041_E1.6580.50
843_I882_M1.6520.50
1001_A1074_M1.6340.49
844_T885_G1.6320.49
999_A1037_I1.6190.48
1065_A1070_H1.6160.48
69_Y72_V1.6080.48
964_R968_I1.6070.48
1035_Q1079_F1.6030.48
177_Q180_S1.6020.47
313_S919_F1.5980.47
630_G690_Y1.5940.47
1016_R1062_L1.5940.47
500_L849_R1.5900.47
733_R967_I1.5900.47
430_F679_P1.5890.47
1067_F1072_I1.5880.47
916_Q921_N1.5680.46
1000_D1034_Q1.5640.46
1030_L1076_F1.5520.45
983_S1043_R1.5490.45
272_L868_G1.5480.45
933_P1024_P1.5400.45
922_F927_I1.5400.45
998_P1076_F1.5370.45
513_D856_E1.5370.45
1009_I1066_G1.5360.45
850_N853_A1.5360.45
945_L958_T1.5290.44
925_G931_E1.5280.44
1042_L1075_P1.5220.44
967_I1031_V1.5200.44
1029_F1054_P1.5190.44
865_L899_K1.5140.44
854_L1046_A1.5080.43
887_L944_D1.5070.43
906_A974_F1.5050.43
995_I1033_L1.5030.43
902_W917_E1.5000.43
751_E880_L1.4910.43
1036_G1076_F1.4910.43
680_L684_L1.4880.42
711_L790_A1.4870.42
323_Q919_F1.4860.42
1000_D1060_H1.4860.42
915_L993_L1.4810.42
999_A1034_Q1.4800.42
923_I993_L1.4790.42
121_S138_L1.4790.42
1012_T1062_L1.4760.42
933_P990_R1.4690.42
494_R635_I1.4690.42
961_D973_A1.4600.41
228_Q232_Q1.4560.41
1036_G1078_P1.4500.41
924_S930_F1.4500.41
412_G995_I1.4490.41
774_I923_I1.4480.41
989_T1041_E1.4460.41
566_L682_A1.4460.41
727_R730_A1.4370.40
323_Q837_A1.4360.40
109_E934_I1.4350.40
317_G975_I1.4340.40
363_I951_K1.4320.40
480_F543_L1.4300.40
272_L413_Q1.4250.40
1031_V1053_M1.4230.40
1037_I1075_P1.4230.40
996_P1033_L1.4210.40
975_I1062_L1.4200.40
913_F976_T1.4200.40
131_A1028_V1.4200.40
1024_P1063_I1.4180.39
911_L917_E1.4170.39
897_W903_L1.4160.39
969_V1021_I1.4160.39
961_D1031_V1.4140.39
854_L1058_E1.4140.39
914_G925_G1.4060.39
518_T850_N1.4040.39
780_R861_Q1.4030.39
914_G931_E1.4020.39
841_F1006_V1.3940.38
936_I983_S1.3900.38
782_V891_S1.3830.38
382_Q386_Q1.3800.38
775_S896_E1.3770.38
866_T983_S1.3770.38
50_Q186_L1.3760.38
638_C641_L1.3720.37
237_A243_S1.3710.37
1033_L1036_G1.3710.37
963_D986_D1.3680.37
1014_A1075_P1.3660.37
383_L630_G1.3660.37
939_T980_I1.3640.37
951_K1064_L1.3600.37
903_L965_K1.3590.37
538_T985_S1.3570.37
610_L636_L1.3560.37
684_L688_V1.3550.37
978_Q1059_I1.3490.37
523_F811_I1.3470.36
1023_N1072_I1.3440.36
27_Q34_Q1.3430.36
916_Q920_A1.3430.36
862_H885_G1.3390.36
609_P814_W1.3380.36
120_K123_Q1.3370.36
999_A1073_D1.3340.36
905_A917_E1.3340.36
902_W920_A1.3320.36
430_F803_S1.3300.36
317_G721_W1.3290.36
459_F581_L1.3290.36
28_I53_L1.3270.36
946_T1030_L1.3270.36
722_M979_F1.3260.36
1034_Q1074_M1.3230.35
902_W905_A1.3210.35
148_A187_V1.3180.35
939_T946_T1.3170.35
902_W972_K1.3150.35
677_G683_I1.3150.35
1038_Q1056_R1.3120.35
629_L632_L1.3120.35
480_F1086_L1.3100.35
727_R777_Q1.3100.35
50_Q84_R1.3070.35
610_L1013_A1.3060.35
508_G513_D1.3050.35
1040_F1061_Q1.3020.35
915_L1043_R1.3020.35
972_K1043_R1.3020.35
556_G828_P1.3000.35
989_T1052_R1.2990.34
1000_D1076_F1.2970.34
121_S298_Q1.2950.34
867_P932_K1.2940.34
715_Y939_T1.2930.34
921_N925_G1.2920.34
382_Q389_L1.2910.34
872_A991_V1.2900.34
921_N931_E1.2880.34
65_R68_Q1.2870.34
431_W798_W1.2810.34
767_S923_I1.2730.33
915_L966_E1.2720.33
437_P979_F1.2670.33
393_L850_N1.2660.33
1035_Q1077_P1.2650.33
500_L525_S1.2650.33
950_T958_T1.2630.33
720_R777_Q1.2620.33
779_L803_S1.2610.33
897_W917_E1.2580.33
534_V680_L1.2530.33
239_R991_V1.2490.32
490_I859_I1.2460.32
957_T984_L1.2440.32
903_L916_Q1.2410.32
321_R1040_F1.2390.32
715_Y977_E1.2350.32
945_L978_Q1.2310.32
1061_Q1064_L1.2300.32
998_P1001_A1.2250.32
924_S976_T1.2250.32
193_A198_N1.2250.32
1042_L1067_F1.2200.31
724_I867_P1.2180.31
60_K63_L1.2170.31
948_S1048_E1.2160.31
990_R1024_P1.2110.31
965_K989_T1.2110.31
32_L111_L1.2100.31
258_I386_Q1.2070.31
901_Q905_A1.2030.31
110_I923_I1.2020.31
124_A208_S1.2000.31
901_Q917_E1.2000.31
897_W920_A1.1980.31
440_I539_L1.1960.30
1015_R1044_I1.1930.30
621_D626_S1.1920.30
413_Q578_P1.1910.30
585_H720_R1.1910.30
850_N911_L1.1900.30
646_L829_I1.1850.30
967_I977_E1.1840.30
855_L860_L1.1820.30
386_Q389_L1.1810.30
429_L433_S1.1760.30
344_R442_W1.1710.30
709_F846_Q1.1700.29
795_I807_F1.1700.29
64_E68_Q1.1680.29
1018_S1068_H1.1670.29
983_S991_V1.1660.29
566_L1051_H1.1650.29
725_Q960_S1.1650.29
298_Q316_L1.1640.29
102_S105_A1.1630.29
407_L959_I1.1630.29
853_A961_D1.1620.29
914_G921_N1.1620.29
268_L985_S1.1610.29
521_T585_H1.1610.29
316_L413_Q1.1560.29
320_L734_A1.1540.29
42_Q45_V1.1520.29
322_A600_Y1.1500.29
117_L208_S1.1470.29
902_W966_E1.1460.29
951_K988_V1.1450.29
229_L908_G1.1380.28
350_Y995_I1.1380.28
61_G65_R1.1360.28
156_E893_I1.1350.28
907_L991_V1.1340.28
999_A1035_Q1.1310.28
777_Q961_D1.1290.28
35_A38_A1.1290.28
168_L172_Q1.1280.28
962_W1053_M1.1280.28
319_A936_I1.1270.28
522_L934_I1.1270.28
757_S760_G1.1240.28
858_A876_I1.1240.28
1074_M1080_Q1.1230.28
265_N730_A1.1200.28
865_L870_G1.1180.28
483_L680_L1.1170.28
441_A642_A1.1130.27
238_E241_L1.1100.27
339_E400_L1.1070.27
996_P1080_Q1.1070.27
352_D1075_P1.1060.27
484_I774_I1.1050.27
1034_Q1064_L1.1050.27
461_Q889_G1.1040.27
897_W972_K1.1030.27
946_T996_P1.1000.27
980_I1083_L1.0990.27
407_L964_R1.0980.27
850_N856_E1.0970.27
572_C928_I1.0970.27
609_P771_L1.0940.27
430_F433_S1.0940.27
733_R945_L1.0920.27
777_Q856_E1.0870.26
979_F1030_L1.0870.26
278_R339_E1.0850.26
837_A841_F1.0810.26
644_V848_V1.0790.26
962_W1077_P1.0770.26
441_A788_L1.0720.26
102_S681_V1.0710.26
155_I159_L1.0710.26
422_N945_L1.0710.26
902_W957_T1.0690.26
897_W966_E1.0670.26
90_M934_I1.0650.26
805_F1080_Q1.0650.26
841_F995_I1.0640.26
120_K124_A1.0630.26
515_F721_W1.0620.26
827_E1079_F1.0600.26
509_K911_L1.0590.26
71_Q587_G1.0560.25
855_L865_L1.0560.25
720_R897_W1.0540.25
853_A911_L1.0540.25
1011_L1028_V1.0530.25
781_L851_L1.0520.25
1013_A1016_R1.0520.25
300_L519_L1.0510.25
46_V538_T1.0510.25
1000_D1030_L1.0500.25
867_P899_K1.0490.25
897_W957_T1.0490.25
345_V895_I1.0480.25
430_F786_L1.0440.25
689_G879_Y1.0440.25
689_G910_G1.0440.25
689_G938_D1.0440.25
689_G947_G1.0440.25
689_G955_R1.0440.25
689_G970_P1.0440.25
689_G971_N1.0440.25
689_G981_N1.0440.25
689_G982_W1.0440.25
879_Y910_G1.0440.25
879_Y938_D1.0440.25
879_Y947_G1.0440.25
879_Y955_R1.0440.25
879_Y970_P1.0440.25
879_Y971_N1.0440.25
879_Y981_N1.0440.25
879_Y982_W1.0440.25
910_G938_D1.0440.25
910_G947_G1.0440.25
910_G955_R1.0440.25
910_G970_P1.0440.25
910_G971_N1.0440.25
910_G981_N1.0440.25
910_G982_W1.0440.25
938_D947_G1.0440.25
938_D955_R1.0440.25
938_D970_P1.0440.25
938_D971_N1.0440.25
938_D981_N1.0440.25
938_D982_W1.0440.25
947_G955_R1.0440.25
947_G970_P1.0440.25
947_G971_N1.0440.25
947_G981_N1.0440.25
947_G982_W1.0440.25
955_R970_P1.0440.25
955_R971_N1.0440.25
955_R981_N1.0440.25
955_R982_W1.0440.25
970_P981_N1.0440.25
970_P982_W1.0440.25
971_N981_N1.0440.25
971_N982_W1.0440.25
66_I455_S1.0420.25
336_L430_F1.0420.25
186_L446_I1.0400.25
371_L375_Q1.0390.25
903_L917_E1.0360.25
941_T989_T1.0350.25
274_Q487_G1.0350.25
437_P1069_A1.0350.25
358_P362_Q1.0350.25
527_L1020_V1.0350.25
433_S989_T1.0340.25
49_L828_P1.0330.25
998_P1030_L1.0330.25
1006_V1060_H1.0320.25
237_A244_T1.0300.25
751_E755_H1.0280.24
901_Q976_T1.0270.24
263_K307_S1.0270.24
900_L942_I1.0270.24
903_L924_S1.0250.24
685_A890_F1.0250.24
322_A352_D1.0250.24
871_Y930_F1.0240.24
839_L842_I1.0220.24
901_Q919_F1.0220.24
948_S961_D1.0210.24
996_P1039_I1.0210.24
543_L828_P1.0190.24
430_F870_G1.0180.24
490_I493_R1.0170.24
720_R856_E1.0170.24
622_D626_S1.0150.24
237_A240_A1.0140.24
67_K146_T1.0130.24
506_K517_L1.0130.24
925_G935_R1.0120.24
278_R450_L1.0120.24
867_P870_G1.0120.24
630_G777_Q1.0100.24
901_Q930_F1.0100.24
211_A978_Q1.0090.24
931_E935_R1.0080.24
194_Q200_R1.0070.24
348_L919_F1.0060.24
762_I1083_L1.0050.24
507_V724_I1.0050.24
942_I1043_R1.0050.24
311_G936_I1.0000.24
185_A847_L1.0000.24
501_E594_S0.9990.24
359_L363_I0.9980.24
714_V1028_V0.9980.24
738_R865_L0.9970.23
518_T720_R0.9970.23
609_P612_M0.9950.23
178_S185_A0.9950.23
57_E61_G0.9930.23
32_L387_R0.9920.23
988_V1018_S0.9920.23
1035_Q1074_M0.9920.23
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539_L606_L0.5370.12
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576_A1046_A0.5340.12
365_Q443_P0.5340.12
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798_W957_T0.5330.12
680_L688_V0.5330.12
856_E900_L0.5330.12
714_V888_V0.5330.12
390_L1076_F0.5320.12
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861_Q1057_H0.5310.12
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536_W612_M0.5300.12
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281_L923_I0.5230.12
352_D407_L0.5220.12
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713_V824_E0.5200.12
116_Q163_T0.5190.12
303_L306_Q0.5190.12
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276_A521_T0.5180.12
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389_L540_G0.5160.12
696_A862_H0.5160.12
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447_A1075_P0.5150.11
727_R872_A0.5150.11
322_A1059_I0.5150.11
437_P856_E0.5150.11
704_S778_S0.5150.11
919_F960_S0.5140.11
296_V417_A0.5140.11
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720_R917_E0.5130.11
324_V932_K0.5130.11
951_K1029_F0.5130.11
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896_E932_K0.5130.11
1040_F1066_G0.5130.11
495_Y950_T0.5120.11
688_V1068_H0.5120.11
108_Q993_L0.5120.11
899_K968_I0.5110.11
1014_A1030_L0.5110.11
844_T918_I0.5110.11
433_S939_T0.5100.11
779_L919_F0.5100.11
968_I1057_H0.5090.11
193_A196_S0.5090.11
902_W927_I0.5090.11
913_F917_E0.5090.11
441_A710_L0.5090.11
794_V944_D0.5080.11
533_P631_R0.5080.11
40_P358_P0.5080.11
1022_D1056_R0.5080.11
565_L866_T0.5070.11
284_S1025_A0.5070.11
1001_A1039_I0.5070.11
913_F941_T0.5070.11
541_Y1059_I0.5070.11
932_K1027_E0.5070.11
47_E238_E0.5060.11
203_L238_E0.5060.11
180_S211_A0.5050.11
480_F951_K0.5050.11
983_S1045_Y0.5050.11
693_T974_F0.5040.11
496_F974_F0.5040.11
71_Q859_I0.5040.11
920_A1060_H0.5030.11
771_L814_W0.5030.11
801_I804_A0.5030.11
135_A138_L0.5030.11
507_V873_I0.5030.11
320_L904_V0.5020.11
422_N654_P0.5010.11
804_A908_G0.5010.11
353_L791_L0.5000.11
145_Q705_V0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
5ajiA 3 0.2376 100 0.808 Contact Map
4hw9A 3 0.2285 100 0.814 Contact Map
3udcA 3 0.2304 100 0.82 Contact Map
1i84S 2 0.0903 99.7 0.944 Contact Map
1c1gA 2 0.2511 99 0.961 Contact Map
4uxvA 1 0.3388 98 0.972 Contact Map
4linA 5 0.2493 97.4 0.975 Contact Map
2dfsA 2 0.168 97 0.976 Contact Map
3ojaB 1 0.1274 96.7 0.977 Contact Map
4cgkA 3 0.3089 96 0.979 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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