WBBK - ECOLI - GREMLIN Contacts

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WBBK - Putative glycosyltransferase WbbK

UniProt:	P37751	Sequences: MGKSIVVVSAVNFTTGGPFTILKKFLAATNNKENVSFIALVHSAKELKESYPWVKFIEFPEVKGSWLKRLHFEYVVCKKLSKELNATHWICLHDITANVVTKKRYVYCHNPAPFYKGILFREILMEPSFFLFKMLYGLIYKINIKKNTAVFVQQFWMKEKFIKKYSINNIIVSRPEIKLSDKSQLTDDDSQFKNNPSELTIFYPAVPRVFKNYELIISAARKLKEQSNIKFLLTISGTENAYAKYIISLAEGLDNVHFLGYLDKEKIDHCYNISDIVCFPSRLETWGLPLSEAKERGKWVLASDFPFTRETLGSYEKKAFFDSNNDDMLVKLIIDFKKGNLKKDISDANFIYRNENVLVGFDELVNFITEEH	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11985
Length:	372 (330)
Sequences:	55262 (47107)
Seq/√Len:	2593.2

ECOLI - WBBK WCAL RFAG GLGA WCAI RFAB WCAC
BACSU - YPJH EPSD YQGM COTSA YTCC EPSF GLGA TUAC TUAH YKVP CGEB TAGE
HALSA - Q9HQQ0 Q9HSW1 Q9HS45 Q9HSV4 Q9HRP8 Q9HQT2 Q9HSV2 Q9HRP9
SULSO - Q97UM0 Q980C8 Q97V80 Q97VL4 Q97XT7 Q97V61 Q7LXM9 Q97ZD3 Q97XU2 Q7LXI2 Q97UU3 Q97XT1 Q97XT8 Q97XF6 Q7LXF8 Q97XT9 Q97XT5 Q97XT6 Q7LXF5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
202_F	274_S	4.581	1.00
230_K	257_H	3.638	1.00
149_A	171_I	3.229	1.00
240_N	244_K	3.103	1.00
277_V	293_A	3.056	1.00
200_T	232_L	2.903	1.00
222_K	330_V	2.761	1.00
232_L	259_L	2.719	1.00
353_R	358_L	2.711	1.00
218_S	246_I	2.640	1.00
198_E	230_K	2.609	1.00
203_Y	213_Y	2.570	1.00
226_Q	334_I	2.503	1.00
224_K	255_N	2.363	1.00
339_G	342_K	2.238	1.00
158_K	162_I	2.193	1.00
218_S	326_D	2.185	1.00
212_N	215_L	2.183	1.00
38_I	86_A	2.128	1.00
199_L	337_K	2.114	1.00
246_I	250_A	2.076	1.00
304_D	309_R	2.070	1.00
38_I	84_L	2.044	1.00
239_E	242_Y	2.037	1.00
332_L	335_D	2.029	1.00
234_T	270_C	2.011	1.00
90_I	107_Y	2.000	1.00
232_L	257_H	1.993	1.00
200_T	230_K	1.986	1.00
222_K	327_D	1.966	1.00
220_A	231_F	1.906	1.00
200_T	273_I	1.904	1.00
351_I	356_N	1.899	1.00
199_L	275_D	1.887	1.00
201_I	276_I	1.866	1.00
312_L	318_K	1.865	1.00
335_D	342_K	1.836	1.00
313_G	316_E	1.827	1.00
317_K	353_R	1.825	1.00
297_G	351_I	1.809	1.00
343_K	347_D	1.806	1.00
335_D	339_G	1.783	1.00
248_S	253_L	1.778	1.00
319_A	332_L	1.769	1.00
212_N	282_R	1.763	1.00
38_I	57_I	1.757	1.00
236_S	240_N	1.746	1.00
221_R	250_A	1.719	1.00
322_D	328_M	1.714	1.00
155_F	158_K	1.708	1.00
304_D	322_D	1.692	1.00
334_I	338_K	1.671	1.00
363_E	367_F	1.670	1.00
262_L	266_K	1.670	1.00
216_I	231_F	1.666	1.00
277_V	298_K	1.648	1.00
155_F	310_E	1.647	1.00
158_K	172_V	1.636	1.00
313_G	353_R	1.630	1.00
204_P	267_I	1.620	1.00
176_E	361_F	1.595	1.00
152_V	170_I	1.594	1.00
220_A	229_I	1.572	1.00
106_V	147_N	1.555	1.00
316_E	349_N	1.537	1.00
319_A	335_D	1.524	1.00
352_Y	357_V	1.516	1.00
216_I	278_C	1.514	1.00
342_K	345_I	1.513	1.00
155_F	172_V	1.503	1.00
328_M	332_L	1.481	1.00
158_K	170_I	1.475	1.00
345_I	349_N	1.462	1.00
313_G	317_K	1.455	1.00
214_E	246_I	1.454	1.00
325_N	328_M	1.450	1.00
23_K	27_A	1.442	1.00
28_A	31_N	1.437	1.00
28_A	362_D	1.429	1.00
215_L	282_R	1.427	1.00
210_F	283_L	1.422	1.00
38_I	55_K	1.422	1.00
223_L	334_I	1.417	1.00
215_L	326_D	1.411	1.00
234_T	259_L	1.400	1.00
33_E	366_N	1.390	1.00
302_A	308_T	1.387	1.00
262_L	270_C	1.386	1.00
266_K	269_H	1.382	1.00
232_L	270_C	1.363	1.00
274_S	298_K	1.363	1.00
321_F	332_L	1.355	1.00
152_V	158_K	1.337	1.00
268_D	296_R	1.335	1.00
263_D	266_K	1.333	1.00
349_N	352_Y	1.332	1.00
200_T	275_D	1.328	1.00
28_A	365_V	1.318	1.00
203_Y	216_I	1.316	1.00
232_L	274_S	1.311	1.00
234_T	262_L	1.297	1.00
326_D	330_V	1.290	1.00
222_K	326_D	1.284	1.00
245_Y	249_L	1.278	1.00
219_A	329_L	1.277	1.00
213_Y	233_L	1.274	1.00
207_P	233_L	1.267	1.00
201_I	278_C	1.263	1.00
202_F	234_T	1.260	1.00
259_L	262_L	1.250	1.00
201_I	333_I	1.249	1.00
246_I	249_L	1.245	1.00
91_C	104_R	1.239	1.00
328_M	331_K	1.209	1.00
155_F	159_E	1.206	1.00
286_W	291_S	1.199	1.00
199_L	276_I	1.191	1.00
272_N	296_R	1.188	1.00
330_V	334_I	1.178	1.00
182_K	296_R	1.170	1.00
282_R	324_N	1.167	1.00
195_N	198_E	1.165	1.00
344_D	348_A	1.159	1.00
21_I	288_L	1.153	1.00
279_F	290_L	1.137	1.00
347_D	350_F	1.131	1.00
244_K	248_S	1.130	1.00
267_I	271_Y	1.130	1.00
214_E	218_S	1.129	1.00
159_E	162_I	1.128	1.00
181_D	268_D	1.122	1.00
363_E	366_N	1.122	1.00
151_F	368_I	1.118	1.00
221_R	225_E	1.117	1.00
342_K	346_S	1.117	1.00
290_L	308_T	1.116	1.00
236_S	261_Y	1.093	1.00
215_L	218_S	1.089	1.00
215_L	303_S	1.084	1.00
153_Q	157_M	1.083	1.00
241_A	244_K	1.075	1.00
199_L	229_I	1.074	1.00
361_F	364_L	1.074	1.00
360_G	363_E	1.072	1.00
321_F	329_L	1.072	1.00
271_Y	296_R	1.072	1.00
297_G	348_A	1.071	1.00
200_T	274_S	1.068	1.00
314_S	332_L	1.065	1.00
241_A	245_Y	1.060	1.00
153_Q	175_P	1.059	1.00
222_K	226_Q	1.056	1.00
336_F	346_S	1.054	1.00
279_F	289_P	1.048	1.00
303_S	321_F	1.042	1.00
199_L	228_N	1.041	1.00
302_A	320_F	1.039	1.00
215_L	324_N	1.037	1.00
298_K	347_D	1.037	1.00
264_K	267_I	1.036	1.00
340_N	343_K	1.033	1.00
259_L	270_C	1.025	1.00
30_N	34_N	1.021	1.00
152_V	161_F	1.021	1.00
331_K	335_D	1.020	1.00
198_E	228_N	1.019	1.00
268_D	272_N	1.017	1.00
319_A	346_S	1.017	1.00
218_S	250_A	1.010	1.00
348_A	352_Y	1.001	1.00
352_Y	356_N	1.000	1.00
208_R	238_T	0.999	1.00
109_H	288_L	0.995	1.00
280_P	329_L	0.986	1.00
75_V	79_K	0.986	1.00
247_I	258_F	0.985	1.00
204_P	271_Y	0.984	1.00
347_D	351_I	0.982	1.00
340_N	344_D	0.981	1.00
294_K	300_V	0.979	1.00
214_E	242_Y	0.977	1.00
222_K	225_E	0.975	1.00
232_L	273_I	0.973	1.00
287_G	308_T	0.972	1.00
159_E	163_K	0.970	1.00
24_K	27_A	0.969	1.00
319_A	336_F	0.968	1.00
290_L	302_A	0.968	1.00
105_Y	151_F	0.965	1.00
152_V	157_M	0.961	1.00
281_S	284_E	0.960	1.00
182_K	268_D	0.957	1.00
201_I	220_A	0.955	1.00
202_F	270_C	0.954	1.00
214_E	282_R	0.949	1.00
302_A	312_L	0.947	1.00
277_V	289_P	0.947	1.00
307_F	310_E	0.944	1.00
216_I	280_P	0.939	1.00
204_P	234_T	0.937	1.00
104_R	147_N	0.935	1.00
153_Q	285_T	0.927	1.00
293_A	298_K	0.927	1.00
247_I	252_G	0.925	1.00
223_L	333_I	0.924	1.00
301_L	321_F	0.923	1.00
206_V	210_F	0.915	1.00
160_K	164_K	0.915	1.00
215_L	329_L	0.912	1.00
223_L	229_I	0.912	1.00
175_P	288_L	0.910	1.00
147_N	150_V	0.908	1.00
178_K	181_D	0.906	1.00
78_K	82_K	0.902	1.00
24_K	361_F	0.900	1.00
160_K	163_K	0.900	1.00
26_L	39_A	0.900	1.00
230_K	255_N	0.900	1.00
217_I	247_I	0.899	1.00
346_S	349_N	0.895	1.00
294_K	350_F	0.893	1.00
220_A	255_N	0.891	1.00
306_P	309_R	0.890	1.00
294_K	317_K	0.889	1.00
16_G	19_F	0.889	1.00
28_A	33_E	0.882	1.00
290_L	300_V	0.881	1.00
344_D	347_D	0.880	1.00
103_K	148_T	0.875	1.00
91_C	106_V	0.870	1.00
203_Y	233_L	0.868	1.00
20_T	24_K	0.862	1.00
319_A	342_K	0.861	1.00
223_L	337_K	0.860	1.00
331_K	334_I	0.855	1.00
284_E	287_G	0.855	1.00
317_K	350_F	0.854	1.00
26_L	30_N	0.853	1.00
242_Y	245_Y	0.851	1.00
38_I	42_H	0.848	1.00
20_T	264_K	0.848	1.00
279_F	308_T	0.847	1.00
364_L	367_F	0.847	1.00
211_K	284_E	0.845	1.00
364_L	368_I	0.839	1.00
301_L	332_L	0.838	1.00
179_L	360_G	0.838	1.00
229_I	337_K	0.836	1.00
223_L	330_V	0.834	1.00
294_K	353_R	0.830	1.00
290_L	311_T	0.830	1.00
271_Y	298_K	0.829	1.00
219_A	330_V	0.827	1.00
218_S	221_R	0.827	1.00
214_E	239_E	0.822	1.00
276_I	336_F	0.821	1.00
242_Y	246_I	0.817	1.00
327_D	331_K	0.816	1.00
221_R	224_K	0.815	1.00
151_F	173_S	0.815	1.00
299_W	347_D	0.814	1.00
156_W	159_E	0.811	1.00
202_F	271_Y	0.809	1.00
80_L	84_L	0.809	1.00
322_D	325_N	0.809	1.00
339_G	343_K	0.807	1.00
278_C	333_I	0.803	1.00
219_A	326_D	0.802	1.00
353_R	359_V	0.800	1.00
312_L	320_F	0.800	1.00
196_P	199_L	0.799	1.00
176_E	364_L	0.799	1.00
315_Y	335_D	0.797	1.00
211_K	289_P	0.795	1.00
21_I	109_H	0.789	1.00
224_K	227_S	0.788	1.00
245_Y	248_S	0.787	1.00
203_Y	280_P	0.784	1.00
24_K	28_A	0.782	1.00
360_G	364_L	0.780	1.00
142_I	146_K	0.776	1.00
218_S	222_K	0.773	1.00
269_H	273_I	0.771	1.00
162_I	170_I	0.769	1.00
262_L	267_I	0.767	1.00
74_Y	78_K	0.766	1.00
304_D	320_F	0.764	1.00
209_V	214_E	0.762	1.00
270_C	273_I	0.762	1.00
264_K	268_D	0.760	1.00
75_V	78_K	0.758	1.00
22_L	92_L	0.757	1.00
276_I	333_I	0.756	1.00
247_I	250_A	0.752	1.00
24_K	178_K	0.752	1.00
36_S	55_K	0.750	1.00
226_Q	330_V	0.748	1.00
247_I	256_V	0.747	1.00
162_I	165_Y	0.746	1.00
346_S	350_F	0.745	1.00
109_H	153_Q	0.745	1.00
204_P	277_V	0.745	1.00
299_W	336_F	0.745	1.00
269_H	272_N	0.745	1.00
105_Y	149_A	0.744	1.00
79_K	83_E	0.744	1.00
314_S	317_K	0.744	1.00
174_R	310_E	0.740	1.00
205_A	213_Y	0.738	1.00
16_G	206_V	0.738	1.00
221_R	252_G	0.737	1.00
203_Y	277_V	0.737	1.00
226_Q	331_K	0.734	1.00
305_F	308_T	0.732	1.00
211_K	281_S	0.730	1.00
88_H	105_Y	0.729	1.00
207_P	240_N	0.728	1.00
90_I	368_I	0.726	1.00
203_Y	231_F	0.725	1.00
275_D	343_K	0.723	1.00
91_C	97_A	0.723	1.00
345_I	348_A	0.722	1.00
153_Q	288_L	0.713	1.00
92_L	109_H	0.713	1.00
138_L	142_I	0.713	1.00
341_L	345_I	0.712	1.00
110_N	210_F	0.712	1.00
178_K	182_K	0.708	1.00
185_L	272_N	0.699	1.00
279_F	287_G	0.699	1.00
336_F	343_K	0.698	1.00
223_L	227_S	0.697	1.00
193_K	196_P	0.697	1.00
20_T	23_K	0.697	1.00
209_V	282_R	0.696	1.00
171_I	367_F	0.695	1.00
195_N	230_K	0.694	1.00
301_L	336_F	0.694	1.00
327_D	330_V	0.694	1.00
226_Q	338_K	0.689	1.00
362_D	366_N	0.689	1.00
287_G	290_L	0.688	1.00
201_I	229_I	0.687	1.00
209_V	239_E	0.686	1.00
171_I	174_R	0.685	1.00
309_R	320_F	0.682	1.00
291_S	359_V	0.679	1.00
30_N	37_F	0.678	1.00
156_W	160_K	0.677	1.00
303_S	329_L	0.676	1.00
21_I	175_P	0.676	1.00
30_N	53_W	0.676	1.00
361_F	365_V	0.674	1.00
335_D	338_K	0.670	1.00
92_L	107_Y	0.664	1.00
138_L	141_K	0.663	1.00
86_A	102_K	0.663	1.00
317_K	349_N	0.661	1.00
174_R	367_F	0.654	1.00
199_L	340_N	0.649	1.00
244_K	258_F	0.648	1.00
286_W	359_V	0.642	1.00
162_I	166_S	0.641	1.00
173_S	368_I	0.640	1.00
30_N	35_V	0.638	1.00
30_N	33_E	0.637	1.00
350_F	353_R	0.637	1.00
21_I	107_Y	0.637	1.00
277_V	300_V	0.637	1.00
314_S	319_A	0.634	1.00
217_I	256_V	0.632	1.00
213_Y	239_E	0.630	1.00
303_S	323_S	0.630	1.00
205_A	211_K	0.627	1.00
174_R	359_V	0.626	1.00
224_K	228_N	0.626	1.00
349_N	353_R	0.623	1.00
220_A	223_L	0.623	1.00
152_V	172_V	0.622	1.00
270_C	274_S	0.621	1.00
219_A	333_I	0.620	1.00
25_F	368_I	0.619	1.00
93_H	96_T	0.619	1.00
107_Y	151_F	0.618	1.00
141_K	145_K	0.617	1.00
278_C	329_L	0.616	1.00
348_A	351_I	0.611	1.00
26_L	37_F	0.611	1.00
199_L	202_F	0.609	1.00
154_Q	157_M	0.608	1.00
31_N	362_D	0.608	1.00
212_N	324_N	0.608	1.00
201_I	337_K	0.605	1.00
27_A	52_P	0.604	1.00
271_Y	289_P	0.600	1.00
179_L	295_E	0.600	1.00
321_F	328_M	0.598	1.00
294_K	347_D	0.597	1.00
21_I	368_I	0.597	1.00
156_W	309_R	0.595	1.00
341_L	344_D	0.594	1.00
46_E	49_E	0.593	1.00
202_F	277_V	0.592	1.00
239_E	243_A	0.592	1.00
247_I	253_L	0.591	1.00
134_M	137_G	0.591	1.00
276_I	301_L	0.589	1.00
202_F	298_K	0.588	1.00
24_K	288_L	0.587	1.00
304_D	323_S	0.586	1.00
86_A	89_W	0.586	1.00
33_E	362_D	0.586	1.00
27_A	31_N	0.584	1.00
305_F	309_R	0.580	1.00
183_S	272_N	0.578	1.00
343_K	346_S	0.577	1.00
217_I	243_A	0.576	1.00
33_E	365_V	0.575	1.00
58_E	61_E	0.574	1.00
215_L	219_A	0.573	1.00
18_P	93_H	0.572	1.00
358_L	363_E	0.571	1.00
216_I	220_A	0.571	1.00
207_P	213_Y	0.570	1.00
15_T	261_Y	0.569	1.00
199_L	343_K	0.569	1.00
312_L	317_K	0.567	1.00
157_M	160_K	0.567	1.00
181_D	264_K	0.567	1.00
155_F	174_R	0.566	1.00
301_L	333_I	0.565	1.00
21_I	25_F	0.565	1.00
12_N	19_F	0.561	1.00
194_N	198_E	0.561	1.00
209_V	242_Y	0.560	1.00
293_A	300_V	0.560	1.00
228_N	255_N	0.557	1.00
200_T	270_C	0.557	1.00
34_N	37_F	0.557	1.00
132_F	135_L	0.555	1.00
275_D	340_N	0.554	1.00
104_R	148_T	0.554	1.00
291_S	311_T	0.553	1.00
243_A	246_I	0.553	1.00
142_I	145_K	0.553	1.00
88_H	103_K	0.553	1.00
180_S	183_S	0.552	1.00
212_N	280_P	0.551	1.00
81_S	86_A	0.550	1.00
80_L	83_E	0.548	1.00
197_S	228_N	0.548	1.00
212_N	303_S	0.547	1.00
244_K	247_I	0.546	1.00
224_K	229_I	0.545	1.00
206_V	211_K	0.542	1.00
149_A	153_Q	0.542	1.00
299_W	343_K	0.541	1.00
91_C	100_V	0.540	1.00
281_S	285_T	0.540	1.00
41_V	56_F	0.540	1.00
35_V	87_T	0.539	1.00
271_Y	277_V	0.539	1.00
173_S	367_F	0.539	1.00
112_A	115_Y	0.537	1.00
234_T	267_I	0.536	1.00
227_S	338_K	0.536	1.00
202_F	232_L	0.535	1.00
25_F	28_A	0.535	1.00
289_P	293_A	0.530	1.00
109_H	175_P	0.530	1.00
89_W	102_K	0.530	1.00
280_P	303_S	0.528	1.00
183_S	186_T	0.526	1.00
287_G	291_S	0.526	1.00
26_L	54_V	0.525	1.00
111_P	114_F	0.525	1.00
27_A	30_N	0.524	1.00
55_K	58_E	0.524	1.00
162_I	169_N	0.523	1.00
207_P	237_G	0.521	1.00
177_I	295_E	0.520	1.00
297_G	347_D	0.519	1.00
231_F	256_V	0.518	1.00
214_E	243_A	0.516	1.00
174_R	286_W	0.516	1.00
52_P	55_K	0.515	1.00
219_A	223_L	0.511	1.00
21_I	92_L	0.510	1.00
276_I	337_K	0.510	1.00
157_M	285_T	0.510	1.00
108_C	111_P	0.508	1.00
149_A	169_N	0.505	1.00
198_E	273_I	0.503	1.00
221_R	249_L	0.503	1.00
281_S	287_G	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2r60A	1	0.9866	100	0.186	Contact Map
4n9wA	1	0.8898	100	0.195	Contact Map
3c48A	2	0.9462	100	0.202	Contact Map
2x6qA	1	0.9328	100	0.214	Contact Map
3froA	3	0.9758	100	0.219	Contact Map
3okpA	1	0.9247	100	0.225	Contact Map
1rzuA	1	0.9704	100	0.227	Contact Map
2iw1A	1	0.9328	100	0.229	Contact Map
2jjmA	4	0.9113	100	0.231	Contact Map
2x0dA	2	0.8844	100	0.238	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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