GREMLIN Database
BTUF - Vitamin B12-binding protein
UniProt: P37028 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12334
Length: 266 (244)
Sequences: 14747 (11488)
Seq/√Len: 735.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
116_A140_L4.0231.00
123_A132_A4.0221.00
120_R133_E4.0081.00
208_S232_L3.8561.00
128_Q131_K3.4921.00
142_Q256_Q3.2781.00
124_P129_P2.9391.00
28_T34_T2.9041.00
120_R136_A2.6911.00
131_K134_Q2.6101.00
144_A148_A2.5231.00
137_Q141_D2.5161.00
73_R76_A2.5121.00
133_E137_Q2.4711.00
180_L185_G2.4441.00
203_Q206_A2.2441.00
177_N181_E2.2081.00
157_R186_E2.2071.00
27_I74_I2.2061.00
140_L144_A2.0301.00
15_L98_A2.0221.00
26_V126_S2.0131.00
96_Q99_S2.0031.00
42_I126_S1.9811.00
134_Q138_S1.9491.00
145_Q149_Q1.9201.00
37_A126_S1.9151.00
36_L119_L1.9121.00
25_R45_V1.9111.00
210_Q232_L1.8801.00
141_D145_Q1.8401.00
231_Q234_I1.8021.00
211_A237_I1.7831.00
94_V98_A1.7781.00
83_I122_L1.7591.00
114_Q117_N1.7561.00
42_I128_Q1.7461.00
144_A147_K1.7131.00
130_D134_Q1.6981.00
211_A235_P1.6551.00
138_S142_Q1.6321.00
46_G77_L1.5591.00
95_D99_S1.5381.00
70_N73_R1.5281.00
105_M121_Q1.5261.00
69_M74_I1.5211.00
131_K135_A1.4981.00
91_E95_D1.4951.00
228_W234_I1.4731.00
117_N120_R1.4721.00
105_M125_W1.4671.00
116_A136_A1.4601.00
28_T37_A1.4571.00
27_I77_L1.4451.00
117_N121_Q1.4431.00
61_E73_R1.4341.00
220_Q223_K1.4251.00
95_D98_A1.4231.00
75_V100_L1.4131.00
40_A128_Q1.4041.00
221_I224_I1.4041.00
84_A106_W1.3911.00
27_I46_G1.3561.00
48_S51_S1.3451.00
148_A151_A1.3301.00
54_P57_A1.3291.00
45_V77_L1.3241.00
120_R140_L1.3071.00
137_Q140_L1.3071.00
129_P133_E1.3051.00
201_R225_K1.3041.00
155_K186_E1.2891.00
38_F57_A1.2821.00
90_A93_Q1.2671.00
219_D223_K1.2581.00
37_A42_I1.2571.00
23_A80_D1.2551.00
130_D133_E1.2431.00
72_E202_E1.2421.00
24_P43_T1.2391.00
19_W22_A1.2351.00
121_Q124_P1.2281.00
218_P222_P1.2091.00
188_I207_R1.2001.00
119_L132_A1.1991.00
203_Q207_R1.1941.00
135_A138_S1.1921.00
141_D144_A1.1871.00
142_Q145_Q1.1831.00
147_K182_V1.1761.00
81_L105_M1.1741.00
146_L255_A1.1731.00
212_I233_K1.1721.00
136_A140_L1.1671.00
143_Y255_A1.1561.00
188_I191_D1.1551.00
53_Y57_A1.1531.00
71_L96_Q1.1501.00
166_P198_Q1.1481.00
161_Q225_K1.1321.00
142_Q253_L1.1311.00
43_T128_Q1.1221.00
24_P80_D1.1211.00
37_A83_I1.1191.00
55_P58_Q1.1091.00
145_Q148_A1.1091.00
157_R161_Q1.1051.00
158_V185_G1.1021.00
82_V101_G1.0981.00
25_R78_K1.0821.00
189_F207_R1.0781.00
172_K181_E1.0741.00
49_S62_Q1.0661.00
157_R210_Q1.0631.00
138_S141_D1.0601.00
154_P157_R1.0581.00
147_K151_A1.0531.00
39_A135_A1.0401.00
47_V62_Q1.0291.00
159_F169_T1.0241.00
107_V121_Q1.0241.00
178_Q181_E1.0201.00
228_W231_Q1.0181.00
158_V180_L1.0161.00
150_Y259_C1.0081.00
213_V258_L0.9991.00
93_Q96_Q0.9991.00
226_Q229_G0.9941.00
123_A136_A0.9881.00
38_F44_P0.9741.00
26_V81_L0.9721.00
120_R124_P0.9641.00
47_V60_I0.9631.00
219_D222_P0.9621.00
186_E190_K0.9611.00
142_Q146_L0.9611.00
44_P60_I0.9491.00
143_Y147_K0.9441.00
19_W103_K0.9431.00
19_W23_A0.9411.00
121_Q125_W0.9351.00
156_K211_A0.9341.00
37_A44_P0.9341.00
191_D207_R0.9291.00
122_L126_S0.9251.00
145_Q256_Q0.9241.00
178_Q182_V0.9241.00
163_G166_P0.9141.00
22_A128_Q0.9091.00
118_A121_Q0.9081.00
40_A135_A0.9041.00
15_L94_V0.9041.00
189_F192_S0.8981.00
139_L182_V0.8971.00
111_S114_Q0.8971.00
159_F162_F0.8891.00
14_F17_P0.8881.00
139_L143_Y0.8871.00
220_Q224_I0.8831.00
132_A136_A0.8731.00
187_N191_D0.8731.00
214_I238_P0.8691.00
243_W257_Q0.8641.00
156_K235_P0.8591.00
98_A104_V0.8581.00
86_R108_D0.8571.00
23_A81_L0.8541.00
35_E250_R0.8431.00
153_K156_K0.8311.00
110_T113_E0.8281.00
172_K192_S0.8281.00
160_L180_L0.8191.00
36_L122_L0.8161.00
40_A126_S0.8151.00
253_L257_Q0.8121.00
21_N24_P0.8111.00
33_N83_I0.8101.00
158_V161_Q0.8081.00
179_V255_A0.8001.00
229_G234_I0.7981.00
63_V77_L0.7971.00
26_V37_A0.7971.00
115_I139_L0.7961.00
155_K159_F0.7961.00
254_A257_Q0.7961.00
120_R123_A0.7911.00
240_T257_Q0.7901.00
28_T44_P0.7891.00
40_A131_K0.7861.00
169_T189_F0.7851.00
213_V237_I0.7801.00
116_A120_R0.7741.00
214_I236_V0.7721.00
133_E136_A0.7711.00
38_F56_Q0.7641.00
135_A139_L0.7631.00
156_K160_L0.7621.00
189_F203_Q0.7591.00
146_L259_C0.7581.00
225_K229_G0.7571.00
83_I125_W0.7531.00
205_L209_P0.7531.00
134_Q137_Q0.7531.00
92_R95_D0.7511.00
173_E177_N0.7491.00
232_L235_P0.7461.00
122_L125_W0.7401.00
149_Q259_C0.7381.00
146_L150_Y0.7381.00
40_A123_A0.7381.00
22_A127_P0.7371.00
17_P105_M0.7341.00
107_V122_L0.7341.00
160_L215_T0.7291.00
30_S85_W0.7271.00
41_G128_Q0.7271.00
174_S177_N0.7261.00
200_S203_Q0.7251.00
113_E117_N0.7251.00
146_L252_I0.7221.00
14_F94_V0.7211.00
46_G64_S0.7171.00
143_Y182_V0.7131.00
116_A139_L0.7091.00
39_A119_L0.7031.00
113_E116_A0.6991.00
29_L82_V0.6991.00
199_V207_R0.6981.00
187_N190_K0.6961.00
35_E248_S0.6941.00
53_Y56_Q0.6931.00
218_P221_I0.6921.00
119_L135_A0.6891.00
126_S129_P0.6871.00
157_R185_G0.6861.00
33_N107_V0.6821.00
170_S196_W0.6811.00
61_E64_S0.6811.00
82_V100_L0.6801.00
107_V118_A0.6781.00
172_K190_K0.6761.00
116_A143_Y0.6671.00
205_L231_Q0.6671.00
157_R162_F0.6651.00
156_K185_G0.6601.00
81_L125_W0.6581.00
25_R80_D0.6581.00
139_L252_I0.6561.00
82_V104_V0.6541.00
185_G211_A0.6531.00
225_K228_W0.6521.00
157_R188_I0.6501.00
149_Q152_D0.6491.00
217_G241_S0.6481.00
74_I97_L0.6411.00
79_P82_V0.6381.00
170_S176_Q0.6371.00
37_A122_L0.6361.00
204_V231_Q0.6331.00
253_L256_Q0.6301.00
136_A139_L0.6291.00
40_A119_L0.6281.00
158_V162_F0.6281.00
161_Q212_I0.6251.00
41_G131_K0.6241.00
183_C255_A0.6191.00
65_T68_G0.6171.00
218_P224_I0.6151.00
116_A182_V0.6141.00
53_Y250_R0.6141.00
109_A118_A0.6131.00
120_R129_P0.6121.00
142_Q252_I0.6111.00
205_L230_E0.6111.00
94_V106_W0.6101.00
34_T47_V0.6081.00
119_L136_A0.6081.00
115_I251_I0.6031.00
113_E140_L0.6021.00
177_N187_N0.5971.00
103_K106_W0.5931.00
22_A80_D0.5911.00
148_A152_D0.5901.00
245_E248_S0.5901.00
173_E194_V0.5881.00
156_K162_F0.5881.00
157_R160_L0.5871.00
156_K161_Q0.5871.00
71_L74_I0.5871.00
240_T243_W0.5861.00
40_A132_A0.5861.00
143_Y146_L0.5861.00
179_V251_I0.5861.00
33_N122_L0.5821.00
72_E75_V0.5821.00
75_V202_E0.5821.00
247_A254_A0.5821.00
169_T199_V0.5811.00
189_F199_V0.5801.00
212_I228_W0.5791.00
250_R253_L0.5781.00
71_L93_Q0.5781.00
215_T239_L0.5781.00
29_L63_V0.5761.00
243_W253_L0.5711.00
82_V97_L0.5681.00
255_A259_C0.5671.00
183_C259_C0.5661.00
58_Q61_E0.5651.00
159_F209_P0.5631.00
177_N190_K0.5581.00
20_L103_K0.5531.00
147_K183_C0.5531.00
98_A103_K0.5521.00
34_T38_F0.5491.00
28_T33_N0.5491.00
28_T47_V0.5481.00
83_I105_M0.5481.00
115_I119_L0.5471.00
84_A104_V0.5451.00
27_I63_V0.5451.00
146_L149_Q0.5451.00
33_N36_L0.5441.00
227_Y230_E0.5411.00
150_Y153_K0.5411.00
244_F247_A0.5401.00
18_L21_N0.5391.00
73_R77_L0.5351.00
19_W125_W0.5351.00
208_S233_K0.5351.00
168_F198_Q0.5351.00
172_K177_N0.5341.00
64_S69_M0.5341.00
97_L104_V0.5331.00
84_A94_V0.5311.00
46_G63_V0.5301.00
19_W105_M0.5281.00
71_L100_L0.5261.00
110_T114_Q0.5241.00
252_I256_Q0.5241.00
69_M93_Q0.5241.00
172_K191_D0.5241.00
247_A250_R0.5231.00
175_I247_A0.5221.00
155_K161_Q0.5221.00
155_K158_V0.5191.00
243_W246_R0.5191.00
52_D246_R0.5181.00
38_F60_I0.5171.00
143_Y252_I0.5161.00
199_V204_V0.5141.00
147_K184_G0.5121.00
17_P103_K0.5111.00
38_F54_P0.5111.00
96_Q100_L0.5101.00
147_K150_Y0.5101.00
170_S174_S0.5101.00
150_Y155_K0.5091.00
179_V258_L0.5091.00
140_L143_Y0.5081.00
156_K210_Q0.5081.00
72_E76_A0.5061.00
27_I79_P0.5041.00
19_W81_L0.5041.00
94_V104_V0.5010.99
27_I45_V0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1n2zA 3 0.9211 100 0.217 Contact Map
4hn9A 1 0.9925 100 0.219 Contact Map
4pagA 2 0.9812 100 0.238 Contact Map
4m7oA 1 0.9098 100 0.24 Contact Map
4ovkA 1 0.9737 100 0.24 Contact Map
4mlzA 1 0.9812 100 0.246 Contact Map
3pshA 1 0.9699 100 0.251 Contact Map
4n01A 1 0.9887 100 0.256 Contact Map
2q8pA 1 0.9098 100 0.265 Contact Map
4ljsA 1 0.9812 100 0.265 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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