FBPC - ECOLI - GREMLIN Contacts

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FBPC - Fe(3+) ions import ATP-binding protein FbpC

UniProt:	P37009	Sequences: MTQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCGKTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYGLKMLGVPRAELKARVKEALAMVDLEGFEDRFVDQISGGQQQRVALARALILKPKVLLFDEPLSNLDANLRRSMRDKIRELQKQFDITSLYVTHDQSEAFAVSDTVLVMNKGHIMQIGSPQDLYRQPASRFMASFMGDANLFPATFSDGYVDIYGYHLPRPLHFGTQGEGMVGVRPEAITLSDRGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDVGEQYYLEIHPYGMFVLADAA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG12340
Length:	348 (282)
Sequences:	27882 (19300)
Seq/√Len:	1149.3

ECOLI - FBPC CYSA MALK YCJV UGPC POTG POTA PROV YDCT METN MODC DPPF OPPD RBSA YEHX DPPD ARAG YHES YADG DDPD SAPD MACB OPPF UUP GSIA DDPF MLAF FEPC TAUB YTFR HISP CYDC SAPF NIKE YECC SSUB LSRA ETTA MDLB FECE FHUC MSBA MDLA YHDZ PHNC GLNQ YPHE LPTB YOJI CYDD GLTL PHNK THIQ LIVG ZNUC SUFC XYLG PSTB LIVF MODF ARTP ALSA FTSE LOLD YBBA MGLA NIKD BTUD FETA YEJF YDDA PHNL CCMA YNJD YBHF YBIT YHIH
BACSU - MSMX YURJ YVRA OPUAA OPUCA OPUBA OPPD METN YKFD TAGH DPPD YXLF YKPA YHCH YFIL APPD APPF YDBJ YHAQ YCBN YVFR YFMM YDIF OPPF RBSA YTRB ECFA1 YFMR YUSV TCYN SUNT YHEH YFMF FHUC YFIC YFIB YCLP YXDL YKNV YLMA YTLC BCEA YGAD SUFC YKNU ECSA BMRA YHEI ECFA2 YVCR CYDD SKFE TCYC YWJA YXEO NATA GLNQ YKOD CYDC PSTB1 ARTM FTSE SSUB PSTB2 MNTB YJKB YTHP ALBC YKNY YVRO EXPZ YYBJ YCLH ZNUC YHCG YUFO YTRE YYDI
HALSA - Q9HHQ5 Q9HR06 Q9HNZ9 Q9HMX3 BTUDA Q9HMW0 Q9HMT3 Q9HMX2 Q9HRJ6 Q9HMU8 Q9HMG1 Q9HHM2 PSTB2 Q9HRH8 PHNC Q9HSQ7 Q9HNY3 Q9HMG6 Q9HRZ9 Y2317 Q9HRV7 PSTB1 Y1631 Q9HR96 Q9HHR7 Q9HS04 Q9HQA8 Q9HT03 Q9HR21 Q9HMC1 Q9HMW1
SULSO - Q97UY8 Q97YY4 Q97UF2 Q97ZC0 Q97VC1 Q7LX64 Q97UH3 Q97VF5 Q97VK8 Q97VH6 Q97YP3 Q97YP7 Q97YK2 Q97YP8 Q97VK9 Q97X18 Q97VF4 Q97UD4 Q97YQ7 Q97UJ9 Q97U54 Q97Z03 Q980W0 Y1893 Q97UH4 Q97W42 PSTB Q97V01 Q7LXM4 Y2030 Q7LY07 Q97V00 Q97VM2 Q97VY4 Q7LX80 Q97Z56 Q97UG5 Q97UC2 Q97Z96 Q97YP4 Q97ZU0 Q980K5 Q980E6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
33_Q	206_T	3.741	1.00
36_T	204_S	3.739	1.00
219_G	223_D	3.690	1.00
11_N	24_N	3.582	1.00
20_T	23_D	3.501	1.00
8_E	28_T	3.318	1.00
10_R	62_Q	3.275	1.00
122_A	127_E	3.122	1.00
201_F	222_Q	2.670	1.00
13_T	60_E	2.602	1.00
9_L	12_V	2.581	1.00
114_K	118_K	2.578	1.00
220_S	223_D	2.544	1.00
8_E	64_F	2.543	1.00
14_K	51_L	2.511	1.00
35_V	208_L	2.504	1.00
129_F	132_R	2.504	1.00
15_R	20_T	2.461	1.00
10_R	64_F	2.429	1.00
13_T	20_T	2.429	1.00
30_P	33_Q	2.411	1.00
116_R	154_K	2.338	1.00
109_P	112_E	2.330	1.00
12_V	58_P	2.329	1.00
23_D	214_H	2.302	1.00
79_D	156_K	2.266	1.00
216_M	230_S	2.253	1.00
96_G	100_G	2.226	1.00
13_T	24_N	2.167	1.00
96_G	131_D	2.070	1.00
11_N	26_N	2.020	1.00
182_L	188_I	2.003	1.00
14_K	59_S	1.960	1.00
51_L	58_P	1.920	1.00
125_D	174_S	1.872	1.00
49_L	193_V	1.845	1.00
14_K	56_E	1.828	1.00
96_G	118_K	1.800	1.00
49_L	159_L	1.778	1.00
208_L	215_I	1.771	1.00
96_G	114_K	1.751	1.00
116_R	119_E	1.724	1.00
112_E	115_A	1.722	1.00
7_V	157_V	1.705	1.00
111_A	115_A	1.674	1.00
66_D	156_K	1.655	1.00
121_L	130_E	1.624	1.00
15_R	59_S	1.623	1.00
211_N	216_M	1.604	1.00
29_I	35_V	1.603	1.00
92_H	135_D	1.597	1.00
93_M	101_Y	1.597	1.00
256_G	259_L	1.591	1.00
115_A	119_E	1.590	1.00
22_I	51_L	1.578	1.00
52_V	191_L	1.578	1.00
240_A	243_F	1.539	1.00
94_S	97_E	1.537	1.00
50_R	56_E	1.529	1.00
231_R	272_M	1.526	1.00
9_L	27_L	1.521	1.00
27_L	208_L	1.501	1.00
100_G	117_V	1.499	1.00
208_L	218_I	1.479	1.00
112_E	116_R	1.464	1.00
240_A	244_P	1.452	1.00
120_A	152_I	1.452	1.00
223_D	227_Q	1.448	1.00
13_T	23_D	1.429	1.00
50_R	55_L	1.421	1.00
96_G	117_V	1.420	1.00
124_V	148_A	1.403	1.00
36_T	200_A	1.379	1.00
252_V	256_G	1.379	1.00
222_Q	226_R	1.369	1.00
51_L	63_I	1.343	1.00
206_T	217_Q	1.338	1.00
132_R	136_Q	1.333	1.00
35_V	206_T	1.297	1.00
108_V	112_E	1.297	1.00
58_P	63_I	1.293	1.00
25_I	48_I	1.286	1.00
68_E	156_K	1.283	1.00
9_L	52_V	1.283	1.00
118_K	122_A	1.281	1.00
97_E	100_G	1.277	1.00
231_R	235_S	1.274	1.00
160_F	190_S	1.271	1.00
81_C	150_A	1.268	1.00
257_Y	260_P	1.258	1.00
266_G	269_G	1.247	1.00
10_R	26_N	1.246	1.00
119_E	122_A	1.241	1.00
180_R	202_A	1.229	1.00
180_R	203_V	1.214	1.00
209_V	224_L	1.208	1.00
35_V	218_I	1.206	1.00
22_I	25_I	1.204	1.00
94_S	133_F	1.187	1.00
194_T	200_A	1.170	1.00
133_F	136_Q	1.168	1.00
206_T	218_I	1.165	1.00
9_L	48_I	1.156	1.00
252_V	255_Y	1.155	1.00
93_M	98_N	1.145	1.00
95_L	137_I	1.145	1.00
22_I	48_I	1.130	1.00
81_C	155_P	1.129	1.00
93_M	97_E	1.126	1.00
16_F	21_V	1.120	1.00
119_E	123_M	1.117	1.00
297_V	300_M	1.114	1.00
53_A	159_L	1.107	1.00
192_Y	204_S	1.105	1.00
225_Y	228_P	1.104	1.00
90_F	93_M	1.081	1.00
98_N	149_R	1.079	1.00
100_G	113_L	1.070	1.00
242_L	272_M	1.068	1.00
221_P	225_Y	1.065	1.00
231_R	242_L	1.064	1.00
6_F	28_T	1.063	1.00
241_N	244_P	1.060	1.00
235_S	242_L	1.055	1.00
34_M	190_S	1.054	1.00
6_F	31_Q	1.050	1.00
176_R	202_A	1.046	1.00
108_V	113_L	1.042	1.00
256_G	260_P	1.037	1.00
275_V	280_I	1.030	1.00
253_D	256_G	1.021	1.00
204_S	207_V	1.019	1.00
118_K	130_E	1.017	1.00
261_R	265_F	1.011	1.00
283_S	287_E	1.011	1.00
15_R	21_V	1.006	1.00
31_Q	66_D	1.005	1.00
30_P	206_T	0.995	1.00
29_I	189_T	0.994	1.00
33_Q	205_D	0.990	1.00
127_E	130_E	0.989	1.00
154_K	188_I	0.988	1.00
255_Y	259_L	0.975	1.00
215_I	218_I	0.975	1.00
31_Q	156_K	0.970	1.00
100_G	104_K	0.965	1.00
270_E	274_G	0.961	1.00
164_L	179_I	0.960	1.00
251_Y	255_Y	0.959	1.00
15_R	60_E	0.957	1.00
43_C	210_M	0.955	1.00
9_L	63_I	0.944	1.00
15_R	18_S	0.940	1.00
118_K	127_E	0.940	1.00
240_A	274_G	0.940	1.00
12_V	51_L	0.931	1.00
134_V	145_V	0.931	1.00
296_H	299_Y	0.929	1.00
255_Y	258_H	0.925	1.00
110_R	114_K	0.925	1.00
11_N	60_E	0.922	1.00
209_V	228_P	0.909	1.00
97_E	104_K	0.906	1.00
233_M	237_M	0.902	1.00
283_S	286_G	0.896	1.00
48_I	210_M	0.891	1.00
223_D	226_R	0.884	1.00
99_V	121_L	0.884	1.00
14_K	47_T	0.883	1.00
262_P	265_F	0.883	1.00
253_D	257_Y	0.883	1.00
89_L	98_N	0.882	1.00
120_A	148_A	0.881	1.00
284_D	287_E	0.879	1.00
173_R	177_D	0.876	1.00
246_T	270_E	0.875	1.00
221_P	224_L	0.872	1.00
98_N	134_V	0.871	1.00
254_I	257_Y	0.869	1.00
64_F	67_G	0.868	1.00
91_P	135_D	0.867	1.00
219_G	224_L	0.861	1.00
252_V	259_L	0.860	1.00
83_V	150_A	0.857	1.00
222_Q	299_Y	0.856	1.00
253_D	258_H	0.855	1.00
40_P	43_C	0.854	1.00
119_E	154_K	0.854	1.00
55_L	84_F	0.847	1.00
90_F	102_G	0.838	1.00
120_A	151_L	0.836	1.00
226_R	299_Y	0.833	1.00
276_R	280_I	0.831	1.00
101_Y	105_M	0.830	1.00
99_V	102_G	0.828	1.00
99_V	152_I	0.822	1.00
172_R	196_D	0.821	1.00
32_G	187_D	0.820	1.00
255_Y	260_P	0.819	1.00
96_G	130_E	0.819	1.00
113_L	117_V	0.819	1.00
6_F	30_P	0.816	1.00
32_G	189_T	0.815	1.00
211_N	214_H	0.814	1.00
198_S	202_A	0.813	1.00
15_R	19_N	0.811	1.00
51_L	59_S	0.807	1.00
277_P	280_I	0.806	1.00
120_A	124_V	0.802	1.00
8_E	67_G	0.801	1.00
111_A	114_K	0.799	1.00
265_F	269_G	0.793	1.00
177_D	181_E	0.792	1.00
234_A	238_G	0.791	1.00
105_M	110_R	0.789	1.00
253_D	260_P	0.785	1.00
89_L	134_V	0.783	1.00
53_A	82_M	0.780	1.00
65_I	78_R	0.779	1.00
10_R	28_T	0.779	1.00
267_T	270_E	0.778	1.00
274_G	277_P	0.778	1.00
289_S	293_V	0.778	1.00
275_V	279_A	0.778	1.00
6_F	66_D	0.772	1.00
121_L	126_L	0.764	1.00
273_V	276_R	0.763	1.00
37_L	210_M	0.760	1.00
224_L	228_P	0.754	1.00
120_A	123_M	0.754	1.00
46_T	50_R	0.752	1.00
147_L	160_F	0.752	1.00
178_K	181_E	0.750	1.00
294_I	297_V	0.746	1.00
277_P	281_T	0.743	1.00
156_K	188_I	0.741	1.00
227_Q	231_R	0.739	1.00
268_Q	271_G	0.736	1.00
38_L	209_V	0.736	1.00
245_A	271_G	0.735	1.00
24_N	60_E	0.735	1.00
249_D	252_V	0.733	1.00
92_H	133_F	0.731	1.00
179_I	190_S	0.730	1.00
274_G	278_E	0.730	1.00
293_V	296_H	0.728	1.00
159_L	191_L	0.727	1.00
118_K	126_L	0.727	1.00
137_I	145_V	0.727	1.00
269_G	273_V	0.725	1.00
34_M	203_V	0.721	1.00
101_Y	104_K	0.721	1.00
254_I	259_L	0.721	1.00
225_Y	229_A	0.720	1.00
270_E	277_P	0.718	1.00
16_F	56_E	0.718	1.00
157_V	189_T	0.717	1.00
264_H	267_T	0.717	1.00
94_S	98_N	0.717	1.00
282_L	286_G	0.716	1.00
292_C	295_R	0.716	1.00
271_G	274_G	0.715	1.00
102_G	153_L	0.713	1.00
51_L	56_E	0.708	1.00
269_G	277_P	0.707	1.00
99_V	148_A	0.706	1.00
106_L	153_L	0.705	1.00
12_V	63_I	0.704	1.00
80_I	157_V	0.702	1.00
286_G	289_S	0.702	1.00
158_L	188_I	0.699	1.00
164_L	192_Y	0.698	1.00
87_Y	166_N	0.697	1.00
110_R	113_L	0.695	1.00
162_E	195_H	0.691	1.00
253_D	259_L	0.691	1.00
7_V	65_I	0.688	1.00
258_H	262_P	0.686	1.00
265_F	268_Q	0.684	1.00
244_P	272_M	0.681	1.00
240_A	245_A	0.679	1.00
272_M	275_V	0.671	1.00
102_G	149_R	0.670	1.00
81_C	153_L	0.670	1.00
252_V	257_Y	0.662	1.00
228_P	233_M	0.662	1.00
232_F	235_S	0.660	1.00
35_V	191_L	0.660	1.00
169_A	173_R	0.660	1.00
260_P	263_L	0.659	1.00
104_K	110_R	0.658	1.00
37_L	48_I	0.654	1.00
246_T	250_G	0.653	1.00
250_G	253_D	0.651	1.00
174_S	178_K	0.651	1.00
269_G	276_R	0.651	1.00
96_G	99_V	0.648	1.00
198_S	300_M	0.637	1.00
270_E	273_V	0.637	1.00
271_G	275_V	0.633	1.00
288_E	291_R	0.631	1.00
166_N	171_L	0.630	1.00
109_P	113_L	0.627	1.00
263_L	266_G	0.627	1.00
252_V	260_P	0.626	1.00
235_S	239_D	0.623	1.00
276_R	284_D	0.622	1.00
242_L	246_T	0.620	1.00
27_L	218_I	0.619	1.00
177_D	296_H	0.616	1.00
182_L	190_S	0.616	1.00
272_M	276_R	0.614	1.00
269_G	272_M	0.612	1.00
116_R	120_A	0.611	1.00
241_N	245_A	0.607	1.00
244_P	270_E	0.607	1.00
173_R	198_S	0.604	1.00
294_I	299_Y	0.604	1.00
250_G	255_Y	0.604	1.00
90_F	98_N	0.603	1.00
237_M	241_N	0.601	1.00
261_R	264_H	0.601	1.00
15_R	23_D	0.599	1.00
108_V	116_R	0.598	1.00
106_L	110_R	0.597	1.00
37_L	208_L	0.596	1.00
276_R	282_L	0.594	1.00
155_P	158_L	0.593	1.00
211_N	232_F	0.593	1.00
105_M	109_P	0.593	1.00
98_N	101_Y	0.592	1.00
244_P	247_F	0.592	1.00
175_M	178_K	0.592	1.00
170_N	235_S	0.590	1.00
103_L	106_L	0.582	1.00
31_Q	187_D	0.580	1.00
260_P	265_F	0.578	1.00
158_L	190_S	0.577	1.00
13_T	22_I	0.576	1.00
276_R	283_S	0.575	1.00
79_D	153_L	0.575	1.00
277_P	284_D	0.572	1.00
289_S	292_C	0.572	1.00
52_V	159_L	0.572	1.00
221_P	232_F	0.571	1.00
94_S	131_D	0.571	1.00
16_F	47_T	0.570	1.00
121_L	124_V	0.566	1.00
243_F	249_D	0.566	1.00
65_I	80_I	0.565	1.00
238_G	241_N	0.563	1.00
83_V	143_Q	0.563	1.00
114_K	117_V	0.563	1.00
23_D	212_K	0.561	1.00
84_F	87_Y	0.561	1.00
34_M	192_Y	0.561	1.00
117_V	120_A	0.559	1.00
287_E	291_R	0.557	1.00
102_G	106_L	0.557	1.00
277_P	283_S	0.557	1.00
17_G	20_T	0.557	1.00
24_N	214_H	0.556	1.00
266_G	270_E	0.555	1.00
210_M	215_I	0.553	1.00
263_L	267_T	0.552	1.00
207_V	221_P	0.552	1.00
103_L	152_I	0.552	1.00
275_V	282_L	0.548	1.00
104_K	113_L	0.547	1.00
248_S	253_D	0.545	1.00
193_V	196_D	0.545	1.00
102_G	105_M	0.545	1.00
294_I	300_M	0.544	1.00
37_L	191_L	0.543	1.00
200_A	204_S	0.543	1.00
56_E	59_S	0.540	1.00
259_L	262_P	0.536	1.00
121_L	127_E	0.536	1.00
83_V	147_L	0.534	1.00
66_D	78_R	0.534	1.00
192_Y	203_V	0.533	1.00
126_L	129_F	0.532	1.00
209_V	233_M	0.532	1.00
267_T	271_G	0.532	1.00
256_G	261_R	0.531	1.00
95_L	98_N	0.529	1.00
180_R	299_Y	0.529	1.00
249_D	253_D	0.527	1.00
237_M	240_A	0.526	1.00
275_V	278_E	0.526	1.00
54_G	65_I	0.525	1.00
157_V	191_L	0.525	1.00
123_M	181_E	0.521	1.00
125_D	128_G	0.520	1.00
54_G	57_K	0.519	1.00
6_F	189_T	0.518	1.00
14_K	22_I	0.518	1.00
29_I	191_L	0.518	1.00
278_E	282_L	0.518	1.00
281_T	285_R	0.516	1.00
50_R	82_M	0.514	1.00
234_A	237_M	0.514	1.00
282_L	285_R	0.514	1.00
177_D	180_R	0.513	1.00
261_R	268_Q	0.513	1.00
160_F	192_Y	0.513	1.00
251_Y	254_I	0.510	1.00
262_P	266_G	0.510	1.00
245_A	249_D	0.508	1.00
290_Q	293_V	0.508	1.00
125_D	178_K	0.508	1.00
11_N	14_K	0.507	1.00
95_L	145_V	0.507	1.00
177_D	202_A	0.506	1.00
232_F	236_F	0.506	1.00
268_Q	272_M	0.505	1.00
100_G	114_K	0.504	1.00
169_A	176_R	0.504	1.00
167_L	175_M	0.504	1.00
259_L	263_L	0.502	1.00
27_L	48_I	0.502	1.00
113_L	116_R	0.501	1.00
14_K	21_V	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2it1A	1	0.9885	100	0.164	Contact Map
1v43A	2	0.9598	100	0.165	Contact Map
1oxxK	1	0.9741	100	0.169	Contact Map
2yyzA	1	0.9856	100	0.176	Contact Map
3rlfA	2	0.9885	100	0.176	Contact Map
1g291	4	0.9885	100	0.176	Contact Map
3fvqA	2	0.9856	100	0.181	Contact Map
1z47A	2	0.9828	100	0.186	Contact Map
3d31A	2	0.9626	100	0.192	Contact Map
4tquS	2	0.9885	100	0.213	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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