GREMLIN Database
LAST - Uncharacterized tRNA/rRNA methyltransferase LasT
UniProt: P37005 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12309
Length: 228 (221)
Sequences: 1748 (1115)
Seq/√Len: 75.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
63_T66_E4.7321.00
65_A126_L4.0851.00
24_K53_D3.8331.00
92_V157_T3.4511.00
131_T151_Y3.3871.00
30_D59_K3.1921.00
18_A47_V3.1421.00
71_V109_A3.1321.00
9_A114_R2.8461.00
76_A127_A2.7291.00
32_R61_F2.7121.00
62_P66_E2.6021.00
9_A35_D2.5851.00
7_L144_L2.4711.00
175_Q201_Q2.4631.00
64_L111_V2.4221.00
5_I152_C2.3471.00
173_Q209_Q2.3341.00
30_D107_H2.2721.00
180_R216_H2.2291.00
68_L74_T2.1871.00
114_R123_E2.1401.00
4_T32_R2.0871.00
76_A124_L2.0341.00
6_I109_A2.0031.00
112_F148_V1.9921.00
45_R56_D1.9561.00
110_L151_Y1.9291.00
22_A149_M1.9281.00
32_R59_K1.9251.00
44_T55_I1.8561.00
5_I23_M1.8511.00
169_A212_T1.8471.00
35_D62_P1.8371.00
173_Q176_A1.8101.00
177_L204_L1.7911.00
144_L148_V1.7611.00
184_L223_E1.7471.00
176_A216_H1.6911.00
178_R205_G1.6701.00
74_T111_V1.6501.00
14_E43_A1.6101.00
183_T223_E1.5970.99
187_T223_E1.5740.99
92_V154_Q1.5440.99
26_M149_M1.5240.99
4_T30_D1.5240.99
28_F152_C1.5020.99
73_F129_V1.4690.99
7_L148_V1.4600.99
19_A148_V1.4500.99
120_T123_E1.4480.99
75_V94_L1.4350.99
79_A82_R1.4340.99
184_L222_I1.4090.99
6_I71_V1.3930.99
8_V119_L1.3740.98
34_V67_S1.3740.98
70_D172_H1.3720.98
188_L221_D1.3640.98
185_L218_L1.3580.98
111_V127_A1.3560.98
203_R207_L1.3560.98
184_L219_L1.3470.98
38_A41_E1.3360.98
212_T216_H1.3220.98
11_A116_D1.3190.98
52_G56_D1.3180.98
64_L68_L1.3130.98
23_M28_F1.3030.98
188_L222_I1.2920.98
74_T171_Q1.2860.98
112_F144_L1.2800.97
89_A129_V1.2690.97
128_D171_Q1.2490.97
89_A97_L1.2460.97
177_L212_T1.2170.97
170_D173_Q1.2150.97
72_D107_H1.2100.96
33_I36_S1.2030.96
35_D63_T1.1980.96
91_P151_Y1.1830.96
167_A209_Q1.1790.96
71_V74_T1.1730.96
72_D106_S1.1720.96
172_H176_A1.1660.96
156_A160_Q1.1580.95
45_R52_G1.1490.95
68_L71_V1.1490.95
24_K51_S1.1350.95
78_T130_L1.1230.95
75_V131_T1.1210.95
14_E47_V1.1200.95
97_L129_V1.1140.94
200_L218_L1.1100.94
32_R70_D1.1070.94
94_L131_T1.1040.94
101_K106_S1.1020.94
117_S120_T1.0870.94
5_I110_L1.0860.94
18_A25_T1.0780.93
13_A41_E1.0710.93
47_V146_Q1.0330.92
114_R120_T1.0210.91
188_L218_L1.0160.91
4_T61_F1.0050.91
185_L188_L1.0010.90
8_V114_R1.0000.90
10_P33_I0.9990.90
177_L181_A0.9880.90
44_T58_I0.9870.90
4_T71_V0.9830.90
135_M139_Y0.9780.89
221_D224_K0.9680.89
29_S57_N0.9530.88
36_S60_V0.9520.88
70_D107_H0.9510.88
182_M197_V0.9500.88
209_Q213_A0.9420.88
79_A140_P0.9410.87
128_D174_L0.9390.87
26_M135_M0.9370.87
220_H224_K0.9300.87
7_L19_A0.9290.87
39_H60_V0.9140.86
99_E158_L0.9130.86
203_R215_L0.9120.86
8_V34_V0.8960.85
82_R85_Y0.8920.85
74_T127_A0.8920.85
207_L215_L0.8730.83
182_M186_T0.8690.83
78_T86_H0.8690.83
142_L149_M0.8670.83
176_A180_R0.8660.83
16_I39_H0.8630.83
92_V158_L0.8580.82
75_V129_V0.8550.82
18_A145_G0.8520.82
19_A145_G0.8450.81
61_F66_E0.8400.81
177_L219_L0.8350.80
17_G21_R0.8250.80
48_A52_G0.8230.79
12_R47_V0.8200.79
125_A198_D0.8170.79
25_T47_V0.8150.79
6_I61_F0.8100.78
13_A16_I0.8080.78
67_S70_D0.8010.78
4_T107_H0.7990.77
98_L111_V0.7910.77
24_K136_V0.7860.76
13_A40_L0.7850.76
97_L100_E0.7830.76
176_A179_E0.7790.76
5_I28_F0.7730.75
14_E149_M0.7720.75
97_L101_K0.7660.74
13_A39_H0.7640.74
177_L215_L0.7620.74
77_T151_Y0.7590.74
28_F145_G0.7500.73
9_A120_T0.7480.73
117_S208_E0.7460.72
175_Q179_E0.7460.72
202_Q208_E0.7450.72
3_I28_F0.7370.72
15_N21_R0.7360.71
24_K54_I0.7310.71
59_K66_E0.7250.70
93_E96_P0.7240.70
207_L212_T0.7240.70
135_M140_P0.7220.70
122_E125_A0.7220.70
102_S108_A0.7220.70
20_A44_T0.7150.69
24_K29_S0.7070.68
40_L45_R0.7030.68
22_A26_M0.7020.68
136_V139_Y0.6970.67
8_V71_V0.6950.67
126_L175_Q0.6940.67
88_Y130_L0.6930.67
51_S139_Y0.6930.67
128_D205_G0.6930.67
24_K139_Y0.6910.67
170_D174_L0.6900.66
73_F101_K0.6890.66
20_A55_I0.6880.66
33_I60_V0.6880.66
25_T149_M0.6880.66
16_I19_A0.6870.66
98_L110_L0.6840.66
154_Q157_T0.6840.66
79_A121_N0.6830.66
178_R201_Q0.6800.65
196_L199_W0.6780.65
75_V97_L0.6760.65
50_G139_Y0.6740.65
11_A141_S0.6710.64
179_E183_T0.6710.64
23_M152_C0.6680.64
20_A121_N0.6600.63
34_V61_F0.6590.63
211_D216_H0.6520.62
13_A45_R0.6470.61
167_A170_D0.6470.61
81_S85_Y0.6470.61
26_M156_A0.6410.61
41_E46_W0.6400.61
55_I58_I0.6400.61
3_I155_L0.6390.61
135_M142_L0.6370.60
23_M54_I0.6350.60
5_I148_V0.6330.60
206_L211_D0.6330.60
136_V150_V0.6310.60
96_P100_E0.6290.59
198_D205_G0.6290.59
26_M142_L0.6280.59
77_T153_Y0.6270.59
102_S105_M0.6250.59
32_R71_V0.6220.58
74_T128_D0.6150.58
95_V155_L0.6150.58
64_L126_L0.6150.58
99_E159_I0.6140.57
178_R197_V0.6130.57
190_V214_M0.6120.57
80_R142_L0.6120.57
139_Y146_Q0.6120.57
27_G136_V0.6110.57
186_T191_A0.6070.57
61_F70_D0.6060.57
69_H172_H0.6000.56
42_P46_W0.5980.56
90_T96_P0.5980.56
43_A47_V0.5970.55
52_G116_D0.5970.55
91_P153_Y0.5940.55
199_W210_R0.5940.55
50_G136_V0.5900.55
23_M153_Y0.5890.54
105_M179_E0.5870.54
29_S138_D0.5850.54
174_L212_T0.5850.54
80_R141_S0.5840.54
6_I67_S0.5820.54
25_T135_M0.5820.54
41_E49_H0.5810.53
154_Q217_R0.5800.53
78_T124_L0.5770.53
78_T126_L0.5770.53
204_L222_I0.5750.53
4_T59_K0.5750.53
9_A117_S0.5710.52
160_Q163_A0.5700.52
125_A130_L0.5670.52
39_H45_R0.5650.51
131_T200_L0.5650.51
68_L171_Q0.5640.51
65_A111_V0.5640.51
16_I70_D0.5640.51
3_I102_S0.5630.51
141_S144_L0.5630.51
19_A152_C0.5580.51
196_L213_A0.5570.50
95_V158_L0.5570.50
26_M152_C0.5570.50
86_H130_L0.5560.50
133_V150_V0.5560.50
20_A79_A0.5540.50
180_R223_E0.5540.50
55_I76_A0.5530.50
89_A92_V0.5530.50
8_V123_E0.5510.50
180_R184_L0.5510.50
29_S136_V0.5500.50
11_A37_Q0.5490.49
94_L98_L0.5470.49
14_E39_H0.5460.49
25_T220_H0.5450.49
85_Y202_Q0.5430.49
128_D201_Q0.5430.49
124_L132_G0.5380.48
28_F153_Y0.5380.48
141_S196_L0.5340.48
167_A208_E0.5330.47
39_H44_T0.5330.47
29_S54_I0.5320.47
90_T93_E0.5320.47
73_F128_D0.5310.47
92_V96_P0.5310.47
181_A218_L0.5300.47
57_N104_W0.5300.47
174_L178_R0.5280.47
9_A144_L0.5280.47
4_T70_D0.5270.47
74_T109_A0.5270.47
68_L109_A0.5260.47
31_L133_V0.5260.47
135_M156_A0.5250.46
47_V145_G0.5250.46
179_E182_M0.5240.46
186_T189_A0.5240.46
191_A197_V0.5220.46
68_L111_V0.5200.46
24_K133_V0.5200.46
62_P65_A0.5180.46
64_L123_E0.5180.46
142_L150_V0.5170.45
9_A37_Q0.5150.45
23_M31_L0.5150.45
13_A38_A0.5150.45
99_E102_S0.5150.45
42_P45_R0.5130.45
22_A51_S0.5130.45
204_L207_L0.5110.45
190_V196_L0.5110.45
200_L204_L0.5100.45
4_T109_A0.5080.44
11_A144_L0.5080.44
137_A145_G0.5060.44
30_D71_V0.5060.44
19_A25_T0.5050.44
26_M53_D0.5040.44
62_P163_A0.5030.44
156_A203_R0.5020.44
18_A149_M0.5010.43
27_G156_A0.5010.43
102_S106_S0.5000.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3onpA 2 0.693 100 0.322 Contact Map
4cndA 2 0.7105 100 0.323 Contact Map
3ilkA 2 0.9825 100 0.325 Contact Map
4cngA 2 0.6623 100 0.361 Contact Map
3ic6A 2 0.7895 100 0.504 Contact Map
3ktyA 2 0.6974 100 0.551 Contact Map
3dcmX 2 0.6711 100 0.595 Contact Map
1zjrA 2 0.7632 100 0.614 Contact Map
1v2xA 2 0.7281 100 0.618 Contact Map
4pzkA 2 0.6711 100 0.629 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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