GREMLIN Database
OSMF - Putative osmoprotectant uptake system substrate-binding protein OsmF
UniProt: P33362 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG12012
Length: 305 (284)
Sequences: 2935 (2151)
Seq/√Len: 127.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
282_S288_L5.2301.00
136_Q229_G4.2411.00
148_A236_P4.0731.00
272_D275_T3.9531.00
134_V144_L3.4251.00
145_T231_Q3.2801.00
119_I251_E3.1681.00
295_A299_K3.1631.00
138_V142_N3.1071.00
136_Q231_Q2.8261.00
136_Q140_E2.8171.00
163_A213_V2.5981.00
282_S296_D2.5751.00
30_G76_L2.4891.00
102_Q106_E2.4771.00
45_L57_N2.4691.00
275_T279_L2.4681.00
133_A218_A2.4321.00
270_S301_K2.4171.00
275_T301_K2.3111.00
28_K56_V2.3101.00
251_E255_R2.2741.00
226_A278_Q2.2511.00
213_V216_A2.2151.00
117_K251_E2.1151.00
118_L248_V2.1111.00
69_G72_T2.0901.00
26_P54_P2.0661.00
122_T265_Q2.0611.00
26_P56_V2.0171.00
177_A239_V2.0111.00
42_N46_Q2.0031.00
46_Q295_A1.9581.00
146_S234_S1.8771.00
179_E185_K1.8731.00
283_I293_V1.8711.00
296_D300_Q1.8591.00
51_H266_P1.8391.00
132_I215_A1.8351.00
270_S297_Y1.8031.00
282_S286_E1.7981.00
288_L296_D1.7901.00
47_V263_W1.7881.00
113_S251_E1.7851.00
31_S41_G1.7691.00
39_L283_I1.7391.00
288_L292_K1.7351.00
146_S236_P1.7181.00
145_T234_S1.7151.00
47_V267_V1.6691.00
93_E111_L1.6451.00
100_N103_Q1.6411.00
155_Q159_T1.6001.00
145_T149_D1.5801.00
131_T232_T1.5771.00
134_V138_V1.5701.00
42_N57_N1.5671.00
110_K114_E1.5651.00
271_L279_L1.5261.00
148_A237_Q1.4961.00
113_S119_I1.4781.00
120_W246_A1.4601.00
44_I80_P1.4421.00
119_I261_A1.4411.00
181_A239_V1.4271.00
274_K277_Q1.4271.00
51_H263_W1.4131.00
29_V78_I1.3991.00
91_K111_L1.3981.00
253_V257_Y1.3941.00
86_G108_V1.3901.00
27_V53_V1.3711.00
135_R138_V1.3701.00
47_V303_W1.3701.00
135_R211_S1.3631.00
144_L153_Y1.3591.00
278_Q282_S1.3551.00
254_L260_M1.3551.00
126_A276_L1.3551.00
160_F215_A1.3501.00
131_T220_G1.3491.00
68_R112_D1.3471.00
165_S219_Y1.3361.00
275_T297_Y1.3341.00
28_K58_K1.3051.00
129_T220_G1.3011.00
281_A286_E1.2971.00
44_I264_L1.2741.00
165_S199_T1.2691.00
13_M16_A1.2691.00
35_T284_A1.2671.00
105_Y109_K1.2591.00
46_Q291_K1.2411.00
198_D202_T1.2361.00
142_N153_Y1.2321.00
121_L249_V1.2240.99
292_K296_D1.2110.99
9_G12_V1.1990.99
106_E109_K1.1940.99
289_D292_K1.1850.99
226_A277_Q1.1700.99
127_N273_A1.1640.99
45_L49_E1.1610.99
68_R72_T1.1580.99
291_K295_A1.1560.99
70_A75_E1.1470.99
106_E110_K1.1370.99
41_G57_N1.1210.99
42_N45_L1.1200.99
46_Q50_S1.1090.99
31_S57_N1.1090.99
49_E55_T1.1070.99
154_L184_F1.1020.99
254_L261_A1.0940.99
181_A237_Q1.0930.99
53_V259_Q1.0920.99
144_L149_D1.0870.99
41_G59_V1.0810.99
49_E57_N1.0720.99
149_D152_R1.0710.99
10_S13_M1.0660.99
46_Q49_E1.0640.99
86_G112_D1.0620.99
161_K214_N1.0570.99
33_I60_Q1.0490.98
261_A265_Q1.0470.98
139_A144_L1.0460.98
43_I297_Y1.0450.98
114_E246_A1.0410.98
83_T105_Y1.0380.98
126_A245_P1.0370.98
161_K187_G1.0360.98
42_N291_K1.0300.98
10_S14_L1.0280.98
147_L184_F1.0240.98
121_L247_P1.0220.98
45_L55_T1.0210.98
265_Q268_F1.0190.98
204_K210_T1.0100.98
32_K79_Y1.0060.98
9_G13_M1.0030.98
112_D120_W1.0020.98
172_A176_P0.9870.98
154_L187_G0.9860.98
44_I247_P0.9850.98
103_Q106_E0.9830.98
43_I267_V0.9760.97
150_L215_A0.9740.97
202_T217_M0.9670.97
274_K278_Q0.9660.97
296_D299_K0.9660.97
125_P269_A0.9640.97
37_G80_P0.9640.97
38_A60_Q0.9600.97
211_S214_N0.9550.97
250_R253_V0.9550.97
43_I46_Q0.9480.97
40_L245_P0.9450.97
107_K111_L0.9430.97
140_E234_S0.9430.97
14_L17_V0.9390.97
273_A277_Q0.9370.97
199_T202_T0.9370.97
86_G120_W0.9370.97
260_M263_W0.9360.97
218_A225_V0.9350.97
6_L10_S0.9320.97
164_A168_F0.9200.96
38_A290_A0.9160.96
120_W248_V0.9150.96
109_K113_S0.9150.96
105_Y123_P0.9080.96
77_D250_R0.8990.96
134_V142_N0.8980.96
223_G284_A0.8980.96
8_A12_V0.8940.96
33_I219_Y0.8930.96
131_T147_L0.8930.96
85_N173_D0.8890.96
87_A104_G0.8790.95
135_R214_N0.8650.95
107_K110_K0.8650.95
43_I47_V0.8620.95
71_I118_L0.8580.95
202_T216_A0.8540.95
167_E171_R0.8530.95
271_L276_L0.8520.95
138_V141_K0.8480.94
17_V20_P0.8460.94
31_S59_V0.8440.94
36_E221_T0.8430.94
28_K55_T0.8410.94
290_A294_A0.8380.94
83_T246_A0.8370.94
25_S257_Y0.8320.94
250_R256_E0.8260.94
127_N220_G0.8200.93
253_V260_M0.8200.93
174_A177_A0.8170.93
151_S184_F0.8120.93
70_A76_L0.8120.93
198_D201_V0.8090.93
275_T278_Q0.8080.93
133_A232_T0.8050.93
278_Q281_A0.8040.93
68_R248_V0.7980.92
203_I224_P0.7950.92
209_Q230_L0.7950.92
112_D248_V0.7950.92
31_S37_G0.7840.92
69_G73_S0.7800.91
43_I279_L0.7790.91
137_D141_K0.7770.91
48_L52_G0.7760.91
188_Q191_L0.7720.91
151_S154_L0.7720.91
25_S53_V0.7670.91
224_P284_A0.7670.91
161_K188_Q0.7660.91
151_S183_G0.7570.90
81_E86_G0.7560.90
140_E229_G0.7540.90
249_V253_V0.7520.90
138_V153_Y0.7460.90
138_V160_F0.7460.90
282_S292_K0.7450.90
147_L233_L0.7400.89
113_S117_K0.7400.89
218_A232_T0.7400.89
43_I298_L0.7340.89
165_S217_M0.7300.89
64_T90_F0.7250.88
105_Y246_A0.7190.88
47_V50_S0.7170.88
47_V298_L0.7160.88
35_T283_I0.7150.88
249_V254_L0.7140.87
17_V23_A0.7130.87
78_I264_L0.7120.87
8_A11_L0.7120.87
175_L179_E0.7080.87
252_S256_E0.7070.87
103_Q107_K0.7070.87
265_Q269_A0.7030.87
266_P303_W0.6990.86
7_W14_L0.6980.86
78_I249_V0.6970.86
9_G15_A0.6970.86
121_L261_A0.6960.86
247_P264_L0.6960.86
12_V17_V0.6910.86
163_A207_A0.6900.86
130_W133_A0.6900.86
128_N132_I0.6890.86
6_L11_L0.6890.86
173_D243_Y0.6830.85
143_K152_R0.6820.85
245_P276_L0.6810.85
150_L160_F0.6800.85
165_S202_T0.6790.85
6_L13_M0.6770.85
137_D300_Q0.6770.85
21_L24_A0.6760.84
189_D192_L0.6740.84
281_A285_V0.6720.84
35_T223_G0.6660.84
53_V257_Y0.6650.83
141_K145_T0.6580.83
262_Q266_P0.6570.83
173_D240_Q0.6560.83
139_A149_D0.6530.82
183_G237_Q0.6520.82
29_V44_I0.6520.82
27_V253_V0.6510.82
90_F112_D0.6510.82
34_D82_Y0.6490.82
117_K143_K0.6490.82
96_A107_K0.6480.82
32_K81_E0.6440.81
7_W11_L0.6440.81
153_Y159_T0.6430.81
123_P244_A0.6430.81
175_L186_L0.6430.81
225_V232_T0.6430.81
162_L175_L0.6390.81
93_E107_K0.6380.81
96_A104_G0.6380.81
136_Q145_T0.6360.81
130_W243_Y0.6340.80
182_Y237_Q0.6330.80
262_Q265_Q0.6320.80
153_Y160_F0.6300.80
282_S300_Q0.6300.80
32_K37_G0.6300.80
199_T203_I0.6280.80
223_G227_A0.6270.80
124_A245_P0.6260.80
49_E54_P0.6240.79
178_F239_V0.6230.79
85_N243_Y0.6210.79
12_V16_A0.6210.79
259_Q262_Q0.6200.79
34_D167_E0.6190.79
29_V57_N0.6190.79
133_A225_V0.6180.79
140_E262_Q0.6180.79
61_L66_V0.6180.79
104_G242_I0.6180.79
17_V21_L0.6170.79
209_Q228_L0.6090.78
27_V257_Y0.6080.78
225_V230_L0.6050.77
200_A203_I0.6030.77
226_A274_K0.6020.77
160_F214_N0.6010.77
176_P180_K0.6010.77
34_D85_N0.5980.77
45_L59_V0.5980.77
229_G238_G0.5970.76
208_Q216_A0.5950.76
43_I271_L0.5950.76
48_L260_M0.5930.76
36_E40_L0.5920.76
109_K119_I0.5920.76
226_A229_G0.5920.76
140_E143_K0.5890.75
220_G232_T0.5890.75
27_V77_D0.5880.75
144_L147_L0.5870.75
48_L53_V0.5860.75
168_F217_M0.5860.75
44_I78_I0.5850.75
11_L14_L0.5850.75
134_V215_A0.5830.75
216_A224_P0.5830.75
263_W267_V0.5820.75
138_V214_N0.5790.74
67_V200_A0.5790.74
132_I147_L0.5770.74
151_S155_Q0.5740.74
10_S17_V0.5740.74
188_Q192_L0.5730.73
145_T152_R0.5710.73
86_G90_F0.5700.73
96_A103_Q0.5670.73
141_K237_Q0.5650.72
164_A169_I0.5650.72
106_E152_R0.5640.72
180_K296_D0.5640.72
110_K255_R0.5640.72
12_V15_A0.5630.72
173_D241_P0.5620.72
139_A147_L0.5610.72
288_L293_V0.5600.72
74_G249_V0.5590.72
169_I214_N0.5580.71
179_E211_S0.5570.71
125_P245_P0.5560.71
134_V233_L0.5550.71
138_V231_Q0.5520.71
252_S255_R0.5500.70
84_G243_Y0.5480.70
227_A285_V0.5480.70
132_I217_M0.5460.70
162_L215_A0.5460.70
271_L275_T0.5450.70
32_K85_N0.5440.70
87_A96_A0.5440.70
144_L148_A0.5430.69
224_P228_L0.5410.69
259_Q263_W0.5410.69
64_T89_F0.5410.69
46_Q298_L0.5410.69
117_K255_R0.5400.69
68_R90_F0.5400.69
255_R258_P0.5400.69
90_F120_W0.5400.69
44_I268_F0.5400.69
33_I63_T0.5390.69
32_K167_E0.5390.69
67_V79_Y0.5390.69
133_A230_L0.5380.69
218_A224_P0.5380.69
154_L186_L0.5380.69
43_I294_A0.5380.69
124_A271_L0.5360.68
121_L265_Q0.5340.68
30_G67_V0.5320.68
201_V207_A0.5320.68
137_D140_E0.5310.68
32_K64_T0.5300.68
51_H260_M0.5290.67
8_A15_A0.5290.67
76_L79_Y0.5290.67
32_K67_V0.5280.67
106_E140_E0.5270.67
163_A192_L0.5250.67
50_S299_K0.5240.67
144_L233_L0.5230.67
94_N107_K0.5230.67
128_N131_T0.5210.66
227_A281_A0.5210.66
178_F184_F0.5210.66
132_I178_F0.5200.66
164_A175_L0.5190.66
234_S255_R0.5180.66
152_R180_K0.5170.66
54_P262_Q0.5160.66
129_T132_I0.5140.65
11_L17_V0.5130.65
131_T218_A0.5130.65
139_A231_Q0.5120.65
33_I167_E0.5110.65
114_E300_Q0.5100.65
169_I175_L0.5100.65
90_F94_N0.5080.64
92_D95_D0.5070.64
30_G61_L0.5060.64
64_T67_V0.5050.64
126_A130_W0.5050.64
68_R86_G0.5040.64
35_T293_V0.5020.63
145_T187_G0.5010.63
101_A238_G0.5000.63
62_G66_V0.5000.63
135_R208_Q0.5000.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3o66A 1 0.8852 100 0.298 Contact Map
4ne4A 1 0.9049 100 0.316 Contact Map
3r6uA 1 0.882 100 0.351 Contact Map
1sw5A 1 0.8852 100 0.403 Contact Map
1r9lA 1 0.859 100 0.518 Contact Map
2rinA 1 0.8426 100 0.581 Contact Map
3tmgA 1 0.8131 100 0.625 Contact Map
3l6gA 1 0.7934 100 0.633 Contact Map
2b4lA 1 0.4918 100 0.656 Contact Map
3un6A 1 0.7934 99.6 0.792 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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