GREMLIN Database
LLDD - L-lactate dehydrogenase
UniProt: P33232 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11963
Length: 396 (352)
Sequences: 3519 (2167)
Seq/√Len: 115.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
335_A347_L3.4391.00
341_Q345_A3.4271.00
114_E143_R3.0961.00
105_L116_V2.9861.00
58_L356_K2.9111.00
61_E68_K2.8521.00
316_V358_A2.8481.00
145_K245_D2.8211.00
304_L327_L2.6751.00
261_D265_F2.6511.00
94_K341_Q2.6131.00
366_S369_E2.6121.00
32_E166_R2.5641.00
63_T68_K2.5521.00
47_L362_T2.5431.00
316_V355_M2.5091.00
165_Y311_N2.4831.00
138_R243_F2.4801.00
28_G35_L2.4001.00
134_R236_D2.3611.00
235_K238_E2.3321.00
241_R268_D2.3211.00
365_K369_E2.2841.00
263_V301_I2.1971.00
25_M84_Y2.1301.00
10_R26_D2.1281.00
78_V333_L2.1241.00
261_D264_R2.1221.00
145_K244_W2.0911.00
249_V269_G2.0701.00
302_A324_D2.0661.00
6_A347_L2.0641.00
98_A341_Q2.0601.00
98_A345_A2.0411.00
74_A325_T2.0281.00
134_R239_W1.9971.00
285_S288_R1.9551.00
41_D285_S1.9411.00
73_V315_V1.8911.00
315_V351_I1.8881.00
74_A327_L1.8861.00
316_V370_I1.8781.00
42_L287_A1.8581.00
42_L305_A1.8211.00
67_E150_S1.7961.00
260_R296_A1.7931.00
45_V287_A1.7731.00
141_L243_F1.7601.00
259_A270_I1.7511.00
141_L152_L1.7441.00
259_A297_V1.7441.00
138_R142_E1.7401.00
99_H345_A1.7401.00
256_P292_A1.7381.00
142_E146_A1.7211.00
104_T153_V1.6981.00
352_E356_K1.6931.00
135_G138_R1.6831.00
241_R248_M1.6711.00
14_Q22_F1.6651.00
71_M352_E1.6471.00
75_L96_A1.6321.00
65_F269_G1.6211.00
295_D372_Q1.6101.00
292_A376_V1.5891.00
263_V300_D1.5841.00
36_R40_E1.5521.00
154_F237_L1.5501.00
138_R239_W1.5411.00
23_H170_S1.5391.00
99_H349_N1.5301.00
364_A374_S1.5031.00
294_A303_I1.4971.00
54_N233_S1.4951.00
37_R46_A1.4791.00
284_L289_A1.4761.00
305_A309_I1.4741.00
81_C92_A1.4701.00
282_G317_R1.4681.00
142_E243_F1.4671.00
75_L332_F1.4481.00
145_K246_G1.4411.00
294_A324_D1.4371.00
113_I149_C1.4331.00
60_L355_M1.4241.00
44_E288_R1.4161.00
92_A103_F1.4121.00
75_L348_L1.4041.00
371_T374_S1.4041.00
250_I262_A1.4031.00
294_A299_G1.4011.00
50_R255_D1.3951.00
328_L351_I1.3911.00
95_A341_Q1.3901.00
5_A165_Y1.3861.00
48_R292_A1.3841.00
19_P23_H1.3791.00
38_N274_N1.3791.00
151_T249_V1.3731.00
250_I267_A1.3521.00
254_L289_A1.3511.00
315_V355_M1.3471.00
105_L149_C1.3431.00
48_R255_D1.3401.00
260_R264_R1.3361.00
9_Y346_N1.3321.00
9_Y347_L1.3291.00
22_F26_D1.3261.00
52_L156_V1.3191.00
254_L283_V1.3191.00
260_R300_D1.3091.00
90_V111_C1.3091.00
70_S100_G1.3061.00
8_D169_H1.3051.00
238_E242_D1.2991.00
271_V327_L1.2860.99
11_A14_Q1.2780.99
41_D288_R1.2760.99
58_L360_T1.2720.99
51_I156_V1.2710.99
272_V289_A1.2620.99
123_P150_S1.2570.99
313_L358_A1.2520.99
87_R91_Q1.2480.99
97_D121_K1.2450.99
141_L240_I1.2420.99
71_M348_L1.2390.99
75_L92_A1.2370.99
39_V43_S1.2320.99
62_T367_I1.2220.99
95_A348_L1.2210.99
233_S236_D1.2210.99
310_R331_A1.2190.99
153_V271_V1.2120.99
151_T247_P1.2070.99
124_M149_C1.2040.99
12_A16_I1.1870.99
53_K261_D1.1740.99
364_A370_I1.1670.99
40_E44_E1.1650.99
60_L71_M1.1540.99
304_L325_T1.1520.99
356_K360_T1.1470.99
134_R229_D1.1410.99
238_E265_F1.1400.99
239_W243_F1.1340.99
31_S311_N1.1210.99
359_M370_I1.1170.99
69_L325_T1.1150.99
76_A327_L1.1080.98
234_W262_A1.1000.98
51_I253_I1.0990.98
286_S305_A1.0990.98
239_W242_D1.0950.98
117_A124_M1.0920.98
64_L325_T1.0900.98
87_R94_K1.0850.98
64_L304_L1.0810.98
36_R43_S1.0800.98
47_L316_V1.0730.98
316_V362_T1.0720.98
85_A91_Q1.0580.98
117_A149_C1.0580.98
60_L356_K1.0560.98
75_L78_V1.0540.98
320_A370_I1.0490.98
59_S68_K1.0470.98
17_L337_A1.0430.98
289_A292_A1.0400.98
269_G302_A1.0380.98
167_D170_S1.0380.98
305_A318_M1.0340.98
237_L267_A1.0240.97
156_V250_I1.0210.97
130_V137_M1.0190.97
139_N142_E1.0180.97
271_V304_L1.0170.97
336_L344_V1.0170.97
163_A357_V1.0140.97
319_I367_I1.0110.97
10_R22_F1.0110.97
71_M101_I1.0080.97
142_E145_K1.0050.97
287_A305_A1.0000.97
272_V286_S0.9960.97
33_Y43_S0.9960.97
346_N349_N0.9960.97
84_Y333_L0.9950.97
37_R288_R0.9930.97
37_R44_E0.9890.97
135_G139_N0.9880.97
77_P104_T0.9880.97
160_T357_V0.9850.97
154_F240_I0.9830.97
65_F249_V0.9820.97
342_A345_A0.9790.97
303_I324_D0.9770.97
65_F302_A0.9740.97
59_S366_S0.9720.96
34_T286_S0.9700.96
152_L246_G0.9690.96
30_Y313_L0.9690.96
293_I303_I0.9680.96
73_V348_L0.9660.96
364_A369_E0.9620.96
48_R256_P0.9590.96
272_V303_I0.9580.96
9_Y335_A0.9570.96
335_A343_G0.9560.96
18_P21_L0.9550.96
6_A9_Y0.9540.96
40_E43_S0.9540.96
101_I348_L0.9520.96
34_T282_G0.9430.96
260_R263_V0.9430.96
28_G32_E0.9390.96
30_Y163_A0.9390.96
143_R147_A0.9360.96
70_S352_E0.9310.96
125_W153_V0.9290.95
42_L309_I0.9280.95
309_I326_V0.9260.95
6_A335_A0.9250.95
339_A343_G0.9250.95
259_A293_I0.9230.95
97_D120_I0.9230.95
115_E118_P0.9080.95
92_A332_F0.9070.95
76_A271_V0.9060.95
64_L69_L0.9000.95
291_P295_D0.8940.94
298_K324_D0.8910.94
32_E36_R0.8850.94
316_V374_S0.8820.94
27_G166_R0.8810.94
25_M79_G0.8810.94
294_A298_K0.8740.94
112_P115_E0.8720.94
107_T128_L0.8700.94
299_G324_D0.8700.94
99_H348_L0.8690.94
95_A344_V0.8630.93
256_P296_A0.8610.93
53_K365_K0.8560.93
248_M268_D0.8550.93
257_E260_R0.8500.93
62_T69_L0.8480.93
42_L317_R0.8470.93
67_E125_W0.8430.92
359_M367_I0.8370.92
33_Y282_G0.8370.92
253_I262_A0.8370.92
292_A295_D0.8360.92
69_L101_I0.8340.92
62_T68_K0.8340.92
79_G108_V0.8320.92
29_A162_G0.8300.92
247_P268_D0.8290.92
105_L113_I0.8270.92
263_V299_G0.8250.92
362_T370_I0.8210.91
163_A168_A0.8200.91
272_V293_I0.8190.91
259_A272_V0.8180.91
114_E146_A0.8160.91
110_V178_A0.8090.91
152_L244_W0.8060.91
46_A288_R0.8050.91
295_D298_K0.8020.90
153_V249_V0.8010.90
164_R167_D0.8000.90
6_A351_I0.7980.90
224_L228_F0.7980.90
5_A8_D0.7970.90
84_Y337_A0.7940.90
10_R39_V0.7930.90
30_Y357_V0.7930.90
90_V94_K0.7920.90
138_R242_D0.7910.90
270_I303_I0.7860.90
291_P296_A0.7850.90
253_I259_A0.7780.89
54_N231_S0.7770.89
176_N179_M0.7770.89
349_N353_K0.7760.89
355_M362_T0.7760.89
39_V310_R0.7750.89
237_L248_M0.7750.89
345_A349_N0.7720.89
41_D287_A0.7690.89
162_G361_L0.7680.89
237_L250_I0.7640.88
367_I370_I0.7610.88
162_G167_D0.7590.88
63_T66_N0.7540.88
53_K369_E0.7530.88
4_S169_H0.7520.87
254_L286_S0.7510.87
74_A271_V0.7500.87
244_W248_M0.7500.87
5_A311_N0.7490.87
33_Y36_R0.7490.87
37_R40_E0.7470.87
371_T375_L0.7460.87
113_I147_A0.7410.87
82_G88_G0.7390.87
67_E123_P0.7390.87
240_I248_M0.7340.86
74_A104_T0.7320.86
104_T327_L0.7300.86
267_A301_I0.7260.86
332_F348_L0.7240.85
69_L102_P0.7230.85
130_V232_I0.7220.85
364_A371_T0.7210.85
12_A339_A0.7210.85
286_S289_A0.7200.85
316_V364_A0.7190.85
17_L21_L0.7190.85
23_H167_D0.7190.85
160_T360_T0.7190.85
178_A181_R0.7170.85
264_R300_D0.7140.85
129_Y155_T0.7130.85
346_N350_L0.7120.84
267_A270_I0.7100.84
249_V304_L0.7100.84
7_S11_A0.7090.84
25_M333_L0.7050.84
249_V302_A0.7030.84
107_T126_F0.7020.84
287_A318_M0.7000.83
270_I293_I0.7000.83
241_R245_D0.6990.83
54_N235_K0.6970.83
75_L101_I0.6960.83
319_I370_I0.6920.83
69_L74_A0.6910.83
91_Q336_L0.6900.83
48_R257_E0.6900.83
263_V302_A0.6860.82
272_V297_V0.6850.82
272_V305_A0.6850.82
93_A122_R0.6850.82
30_Y311_N0.6840.82
91_Q341_Q0.6840.82
47_L282_G0.6820.82
95_A99_H0.6820.82
23_H166_R0.6800.82
93_A120_I0.6780.81
184_Q188_H0.6770.81
297_V303_I0.6760.81
69_L302_A0.6750.81
163_A353_K0.6730.81
78_V332_F0.6730.81
61_E70_S0.6710.81
280_L358_A0.6700.81
125_W249_V0.6670.80
71_M355_M0.6650.80
39_V42_L0.6650.80
73_V325_T0.6650.80
7_S39_V0.6640.80
162_G279_Q0.6630.80
73_V351_I0.6620.80
39_V307_S0.6620.80
75_L95_A0.6610.80
63_T302_A0.6600.80
33_Y317_R0.6580.79
299_G302_A0.6570.79
13_A22_F0.6570.79
294_A297_V0.6560.79
289_A293_I0.6530.79
17_L25_M0.6530.79
359_M364_A0.6490.79
31_S165_Y0.6480.78
81_C333_L0.6480.78
85_A89_E0.6480.78
42_L307_S0.6460.78
56_S231_S0.6440.78
256_P289_A0.6430.78
294_A300_D0.6410.78
19_P170_S0.6400.78
177_A181_R0.6380.77
184_Q187_T0.6350.77
81_C89_E0.6350.77
94_K119_A0.6330.77
136_F139_N0.6300.77
82_G111_C0.6300.77
176_N181_R0.6280.76
339_A346_N0.6260.76
293_I296_A0.6260.76
253_I270_I0.6240.76
332_F335_A0.6240.76
114_E147_A0.6220.76
39_V314_D0.6210.75
158_M161_P0.6210.75
220_Y224_L0.6190.75
161_P232_I0.6180.75
13_A17_L0.6180.75
213_K216_G0.6170.75
18_P22_F0.6160.75
335_A344_V0.6160.75
339_A342_A0.6140.75
221_I225_G0.6130.75
45_V317_R0.6120.74
31_S281_D0.6120.74
113_I140_A0.6120.74
76_A104_T0.6110.74
5_A31_S0.6110.74
30_Y165_Y0.6100.74
64_L302_A0.6090.74
124_M150_S0.6090.74
131_L228_F0.6060.74
126_F144_A0.6050.74
79_G82_G0.6030.73
358_A362_T0.6000.73
12_A338_T0.5980.73
17_L84_Y0.5980.73
313_L317_R0.5980.73
177_A180_R0.5970.73
294_A301_I0.5960.72
14_Q338_T0.5950.72
55_M58_L0.5950.72
270_I301_I0.5950.72
11_A15_R0.5950.72
99_H352_E0.5920.72
297_V300_D0.5900.72
12_A15_R0.5890.72
62_T302_A0.5880.71
29_A167_D0.5870.71
257_E261_D0.5860.71
7_S10_R0.5860.71
7_S31_S0.5840.71
355_M367_I0.5840.71
79_G89_E0.5830.71
46_A284_L0.5800.70
328_L347_L0.5800.70
32_E35_L0.5800.70
107_T129_Y0.5770.70
111_C115_E0.5750.70
34_T38_N0.5750.70
271_V301_I0.5740.70
359_M366_S0.5740.70
116_V119_A0.5740.70
79_G158_M0.5740.70
82_G110_V0.5730.70
111_C116_V0.5730.70
74_A304_L0.5700.69
75_L344_V0.5700.69
304_L324_D0.5680.69
236_D239_W0.5680.69
141_L151_T0.5680.69
172_M175_P0.5640.68
50_R261_D0.5630.68
309_I315_V0.5630.68
315_V326_V0.5620.68
53_K374_S0.5600.68
238_E261_D0.5560.67
27_G80_L0.5560.67
51_I258_D0.5540.67
27_G32_E0.5540.67
120_I124_M0.5530.67
64_L125_W0.5500.66
291_P298_K0.5500.66
87_R90_V0.5500.66
220_Y225_G0.5490.66
73_V328_L0.5480.66
305_A326_V0.5480.66
54_N58_L0.5450.66
92_A344_V0.5440.66
263_V267_A0.5430.65
75_L333_L0.5430.65
4_S165_Y0.5420.65
37_R287_A0.5420.65
126_F140_A0.5420.65
108_V228_F0.5410.65
45_V291_P0.5410.65
248_M267_A0.5410.65
328_L331_A0.5400.65
34_T277_G0.5390.65
97_D122_R0.5390.65
127_Q164_R0.5360.64
6_A350_L0.5360.64
131_L137_M0.5340.64
9_Y338_T0.5330.64
105_L124_M0.5320.64
69_L304_L0.5310.64
65_F69_L0.5300.64
50_R257_E0.5290.63
60_L352_E0.5280.63
50_R53_K0.5270.63
279_Q361_L0.5250.63
154_F232_I0.5250.63
257_E296_A0.5240.63
22_F334_Y0.5230.63
78_V81_C0.5230.63
112_P117_A0.5230.63
10_R338_T0.5220.62
158_M224_L0.5220.62
150_S247_P0.5220.62
104_T125_W0.5220.62
38_N286_S0.5200.62
113_I143_R0.5200.62
316_V359_M0.5190.62
250_I301_I0.5180.62
179_M182_Y0.5170.62
167_D279_Q0.5170.62
80_L180_R0.5170.62
122_R149_C0.5160.62
284_L292_A0.5150.61
145_K243_F0.5150.61
254_L284_L0.5130.61
81_C103_F0.5110.61
324_D327_L0.5110.61
21_L84_Y0.5100.61
73_V355_M0.5100.61
216_G220_Y0.5090.61
45_V288_R0.5080.60
151_T246_G0.5070.60
35_L39_V0.5050.60
123_P151_T0.5050.60
95_A320_A0.5040.60
70_S99_H0.5040.60
90_V119_A0.5030.60
364_A373_D0.5020.59
55_M360_T0.5020.59
175_P179_M0.5000.59
42_L45_V0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2nzlA 3 0.8535 100 0.393 Contact Map
1kbiA 4 0.9116 100 0.403 Contact Map
1goxA 4 0.8838 100 0.418 Contact Map
2nliA 4 0.8611 100 0.426 Contact Map
3sgzA 4 0.846 100 0.427 Contact Map
1p4cA 3 0.8914 100 0.439 Contact Map
1vcfA 3 0.6869 100 0.62 Contact Map
2qr6A 3 0.7753 100 0.622 Contact Map
4myaA 3 0.7273 100 0.633 Contact Map
3sr7A 3 0.6641 100 0.638 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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