GREMLIN Database
NRFF - Formate-dependent nitrite reductase complex subunit NrfF
UniProt: P32711 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11949
Length: 127 (119)
Sequences: 1365 (964)
Seq/√Len: 88.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_Q77_E3.6731.00
90_D97_P3.5671.00
98_L107_A2.8291.00
70_M75_K2.7321.00
100_G103_L2.7101.00
31_Q71_V2.7051.00
82_G86_E2.6731.00
45_P59_V2.5771.00
75_K79_E2.4851.00
90_D99_T2.3901.00
50_Q56_N2.1551.00
78_V82_G2.1511.00
98_L103_L2.1321.00
46_Q59_V2.1191.00
118_L122_R2.0131.00
98_L102_T1.9921.00
35_A39_A1.9611.00
50_Q54_E1.8031.00
115_L119_I1.7761.00
76_N79_E1.7721.00
41_Q95_N1.7571.00
66_Q69_S1.6691.00
63_M84_M1.6411.00
32_Q68_Y1.6381.00
41_Q81_I1.6361.00
97_P102_T1.6101.00
28_N31_Q1.6081.00
67_V80_I1.5671.00
49_N91_F1.5281.00
66_Q83_W1.5201.00
37_N77_E1.4991.00
24_W35_A1.4370.99
27_A72_A1.4190.99
48_Q91_F1.4060.99
66_Q70_M1.4000.99
69_S73_E1.3590.99
103_L107_A1.3380.99
40_S51_N1.3220.99
83_W88_Y1.3160.99
70_M73_E1.3150.99
85_T90_D1.2800.99
7_T11_L1.2630.98
81_I93_R1.2270.98
26_F35_A1.2270.98
111_V115_L1.2120.98
80_I84_M1.1380.97
29_P32_Q1.1270.97
26_F68_Y1.1240.97
119_I122_R1.1000.96
114_L118_L1.0790.96
24_W68_Y1.0690.96
39_A52_L1.0530.95
22_D26_F1.0520.95
37_N40_S1.0280.94
31_Q34_Q1.0060.94
49_N94_Y1.0000.94
46_Q58_P0.9970.94
37_N41_Q0.9920.93
8_L11_L0.9750.93
9_L12_F0.9580.92
35_A68_Y0.9420.91
13_T18_A0.9400.91
95_N98_L0.9330.91
21_V25_Q0.9310.91
107_A111_V0.9290.91
75_K80_I0.9200.90
110_V114_L0.9050.90
31_Q72_A0.9040.90
93_R97_P0.8810.88
80_I92_V0.8710.88
122_R125_A0.8680.88
60_A88_Y0.8670.88
71_V84_M0.8560.87
45_P60_A0.8530.87
35_A71_V0.8520.87
67_V92_V0.8440.86
42_L67_V0.8320.85
7_T10_L0.8190.85
38_I84_M0.8170.84
39_A53_L0.8110.84
34_Q71_V0.8000.83
51_N94_Y0.8000.83
15_F114_L0.7980.83
66_Q84_M0.7960.83
10_L13_T0.7930.83
31_Q35_A0.7880.82
106_W116_M0.7730.81
64_R116_M0.7720.81
42_L81_I0.7690.81
119_I123_V0.7670.80
30_Q33_Q0.7630.80
26_F39_A0.7630.80
50_Q64_R0.7600.80
13_T17_H0.7530.79
11_L14_C0.7520.79
35_A53_L0.7410.78
51_N85_T0.7400.78
42_L49_N0.7260.77
36_L53_L0.7160.76
27_A31_Q0.7140.75
72_A120_L0.7050.75
53_L65_H0.6990.74
50_Q93_R0.6980.74
81_I84_M0.6870.73
14_C17_H0.6800.72
83_W87_R0.6720.71
8_L13_T0.6550.69
18_A121_W0.6530.69
19_Q88_Y0.6340.67
109_P112_L0.6330.66
14_C76_N0.6290.66
39_A63_M0.6280.66
54_E116_M0.6240.65
32_Q36_L0.6230.65
8_L15_F0.6210.65
58_P112_L0.6200.65
12_F16_A0.6180.65
13_T16_A0.6170.64
47_C64_R0.6160.64
24_W53_L0.6160.64
39_A42_L0.6090.63
42_L84_M0.6070.63
49_N60_A0.6020.63
25_Q35_A0.6010.62
22_D51_N0.5990.62
59_V94_Y0.5980.62
38_I92_V0.5970.62
23_T65_H0.5930.61
90_D102_T0.5920.61
53_L91_F0.5900.61
56_N61_V0.5900.61
31_Q39_A0.5880.61
60_A74_G0.5880.61
111_V122_R0.5870.61
41_Q88_Y0.5840.60
59_V91_F0.5830.60
113_L116_M0.5790.60
7_T119_I0.5780.59
76_N122_R0.5780.59
88_Y113_L0.5780.59
45_P108_L0.5690.58
26_F94_Y0.5690.58
61_V65_H0.5620.57
14_C19_Q0.5590.57
70_M80_I0.5560.57
115_L118_L0.5560.57
40_S53_L0.5550.56
15_F124_R0.5530.56
52_L63_M0.5520.56
63_M93_R0.5520.56
83_W93_R0.5520.56
107_A110_V0.5500.56
53_L71_V0.5490.56
34_Q68_Y0.5480.55
35_A64_R0.5480.55
117_A121_W0.5460.55
25_Q36_L0.5440.55
48_Q54_E0.5360.54
84_M92_V0.5350.54
42_L92_V0.5350.54
62_S105_L0.5350.54
59_V63_M0.5310.53
59_V62_S0.5310.53
42_L94_Y0.5300.53
35_A61_V0.5230.52
67_V70_M0.5210.52
52_L84_M0.5190.52
29_P33_Q0.5170.51
29_P68_Y0.5120.51
56_N64_R0.5120.51
50_Q101_Q0.5090.50
57_A93_R0.5040.49
35_A46_Q0.5040.49
48_Q62_S0.5030.49
85_T93_R0.5030.49
36_L39_A0.5020.49
7_T15_F0.5020.49
99_T108_L0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2hl7A 1 0.6457 100 0.322 Contact Map
2kw0A 1 0.6378 100 0.327 Contact Map
4pj0V 1 0.5512 61.3 0.919 Contact Map
4xxlA 1 0.4803 56.2 0.921 Contact Map
1fftB 1 0.6142 42.2 0.927 Contact Map
1odfA 1 0.9921 36.3 0.93 Contact Map
3wfdC 1 0.9134 34 0.931 Contact Map
2yevB 1 0.3701 31.4 0.932 Contact Map
2blfB 1 0.5748 30.1 0.933 Contact Map
1c2nA 1 0.6929 29.9 0.933 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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