GREMLIN Database
NRFD - Protein NrfD
UniProt: P32709 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11947
Length: 318 (279)
Sequences: 1977 (1479)
Seq/√Len: 88.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_A156_A3.7511.00
32_V152_M3.7271.00
97_G162_Y3.6531.00
271_G275_P3.6061.00
260_T264_W3.4391.00
275_P301_G3.2331.00
262_W266_G3.1521.00
27_I63_A2.9331.00
107_V155_L2.7711.00
72_V81_F2.7171.00
260_T263_F2.7081.00
263_F266_G2.6691.00
21_Y71_L2.6441.00
195_A295_C2.5201.00
199_I292_L2.4641.00
77_R80_T2.3601.00
22_L167_L2.3341.00
275_P278_L2.3151.00
296_G300_V2.2141.00
61_P105_M2.1251.00
72_V78_P2.0591.00
28_S104_Y2.0101.00
263_F267_V1.9991.00
17_P73_F1.9451.00
192_S302_V1.9301.00
270_L273_I1.9281.00
97_G101_F1.8891.00
21_Y162_Y1.8811.00
100_L158_L1.8771.00
18_I21_Y1.8041.00
270_L297_A1.7901.00
274_V297_A1.7861.00
61_P109_V1.7811.00
19_A307_F1.7571.00
71_L97_G1.7551.00
256_G260_T1.7431.00
287_G290_A1.7341.00
261_W265_L1.7291.00
284_R290_A1.7231.00
82_W86_F1.7051.00
187_F227_G1.6971.00
93_V96_M1.6741.00
246_K250_L1.6541.00
27_I196_V1.6201.00
74_H163_T1.6161.00
65_I105_M1.6101.00
97_G163_T1.6071.00
21_Y97_G1.5901.00
103_L155_L1.5871.00
28_S64_V1.5841.00
246_K249_A1.5841.00
191_S298_S1.5541.00
222_I229_I1.5521.00
257_G264_W1.5321.00
269_G277_L1.5191.00
107_V152_M1.5121.00
34_L194_A1.5061.00
31_L197_A1.4921.00
271_G297_A1.4801.00
31_L60_G1.4700.99
26_G163_T1.4550.99
22_L97_G1.4460.99
30_G193_G1.4440.99
228_E235_F1.4390.99
260_T266_G1.4370.99
269_G274_V1.4330.99
75_L81_F1.3940.99
251_V255_G1.3930.99
259_W266_G1.3920.99
266_G271_G1.3900.99
68_L101_F1.3830.99
233_V236_F1.3750.99
21_Y164_G1.3610.99
185_F309_I1.3520.99
71_L162_Y1.3500.99
104_Y108_L1.3300.99
56_T104_Y1.3290.99
104_Y159_L1.3290.99
65_I68_L1.3150.99
111_W149_E1.2890.99
160_G186_L1.2860.99
74_H101_F1.2690.98
12_L311_Y1.2650.98
33_T156_A1.2630.98
78_P81_F1.2620.98
64_V104_Y1.2380.98
191_S221_E1.2380.98
218_H291_V1.2360.98
26_G188_S1.2290.98
22_L68_L1.2190.98
16_W19_A1.2180.98
184_L188_S1.2170.98
249_A252_A1.2160.98
266_G269_G1.2120.98
83_K86_F1.2090.98
20_I303_L1.2080.98
160_G189_G1.2070.98
289_P293_A1.1930.98
252_A256_G1.1830.98
215_Q269_G1.1720.97
100_L159_L1.1710.97
251_V254_L1.1710.97
32_V104_Y1.1530.97
64_V105_M1.1480.97
278_L294_A1.1480.97
19_A310_L1.1430.97
300_V304_M1.1420.97
147_G150_T1.1330.97
238_G242_G1.1330.97
262_W304_M1.1290.97
270_L274_V1.1210.97
64_V159_L1.1170.97
269_G283_N1.1150.97
55_T201_M1.1080.96
290_A294_A1.1010.96
32_V108_L1.0940.96
188_S228_E1.0920.96
57_L111_W1.0850.96
264_W267_V1.0830.96
36_V41_F1.0830.96
222_I276_M1.0760.96
285_S291_V1.0690.96
179_P183_V1.0670.96
159_L163_T1.0650.95
22_L71_L1.0620.95
88_Y95_S1.0570.95
32_V57_L1.0560.95
231_L234_A1.0560.95
36_V149_E1.0540.95
17_P74_H1.0480.95
187_F224_V1.0460.95
75_L85_M1.0400.95
68_L72_V1.0340.95
188_S306_R1.0340.95
262_W274_V1.0180.94
36_V153_L1.0140.94
302_V305_L1.0140.94
228_E231_L1.0130.94
199_I295_C1.0110.94
161_A186_L1.0090.94
275_P279_K1.0080.94
18_I310_L1.0060.94
188_S305_L1.0040.94
84_L98_V1.0030.94
274_V300_V1.0010.94
84_L95_S1.0000.94
28_S60_G0.9980.94
26_G306_R0.9890.93
229_I267_V0.9740.93
97_G305_L0.9710.93
60_G108_L0.9680.93
222_I280_P0.9680.93
24_L63_A0.9650.92
41_F44_Q0.9630.92
101_F108_L0.9560.92
97_G167_L0.9550.92
67_G306_R0.9510.92
278_L284_R0.9420.91
94_M193_G0.9400.91
232_L236_F0.9390.91
160_G166_L0.9360.91
100_L162_Y0.9350.91
15_D74_H0.9320.91
69_L72_V0.9230.91
277_L296_G0.9210.91
68_L94_M0.9200.90
307_F311_Y0.9200.90
229_I233_V0.9180.90
57_L115_I0.9170.90
59_V196_V0.9120.90
12_L173_Y0.9100.90
157_V251_V0.9080.90
107_V151_L0.9070.90
266_G304_M0.9060.90
71_L78_P0.9020.90
58_I112_L0.9000.90
33_T153_L0.8990.89
251_V263_F0.8880.89
17_P310_L0.8880.89
100_L156_A0.8870.89
69_L73_F0.8870.89
106_V110_L0.8790.88
72_V101_F0.8740.88
233_V237_V0.8740.88
80_T83_K0.8690.88
62_G232_L0.8620.87
17_P21_Y0.8620.87
65_I109_V0.8620.87
143_P146_R0.8610.87
83_K87_H0.8570.87
76_T185_F0.8560.87
61_P69_L0.8550.87
98_V163_T0.8530.87
165_F182_P0.8520.87
71_L74_H0.8500.87
303_L307_F0.8490.87
72_V75_L0.8470.86
29_A189_G0.8460.86
259_W262_W0.8430.86
239_L242_G0.8420.86
203_I227_G0.8360.86
225_V272_L0.8340.86
76_T102_Q0.8270.85
260_T273_I0.8270.85
99_M102_Q0.8250.85
234_A238_G0.8200.85
228_E305_L0.8200.85
72_V166_L0.8180.85
216_F219_R0.8140.84
162_Y167_L0.8120.84
74_H173_Y0.8090.84
289_P292_L0.8070.84
218_H221_E0.8070.84
22_L162_Y0.8060.84
35_A60_G0.8040.83
94_M166_L0.7970.83
221_E283_N0.7950.83
61_P108_L0.7920.83
276_M279_K0.7910.82
26_G305_L0.7910.82
263_F271_G0.7910.82
248_R251_V0.7890.82
199_I299_L0.7870.82
268_A272_L0.7870.82
261_W266_G0.7860.82
147_G151_L0.7860.82
264_W268_A0.7830.82
153_L186_L0.7820.82
268_A275_P0.7820.82
280_P283_N0.7820.82
257_G263_F0.7820.82
210_H230_F0.7810.82
215_Q286_S0.7730.81
160_G163_T0.7720.81
107_V118_H0.7690.81
197_A217_V0.7690.81
268_A271_G0.7650.80
151_L155_L0.7650.80
271_G301_G0.7650.80
65_I220_M0.7650.80
164_G182_P0.7610.80
191_S225_V0.7610.80
166_L188_S0.7590.80
273_I293_A0.7580.80
235_F239_L0.7580.80
96_M100_L0.7570.80
191_S301_G0.7560.80
26_G97_G0.7560.80
107_V148_L0.7530.79
59_V299_L0.7530.79
243_D289_P0.7480.79
202_A292_L0.7460.79
256_G270_L0.7450.79
13_V90_F0.7440.78
104_Y160_G0.7440.78
215_Q222_I0.7410.78
225_V290_A0.7370.78
36_V106_V0.7330.77
282_V285_S0.7320.77
111_W152_M0.7320.77
101_F163_T0.7280.77
249_A253_A0.7280.77
229_I272_L0.7260.77
221_E298_S0.7260.77
80_T176_L0.7240.77
97_G160_G0.7230.76
231_L238_G0.7200.76
195_A299_L0.7160.76
250_L257_G0.7160.76
21_Y26_G0.7140.76
293_A297_A0.7100.75
258_F262_W0.7050.75
85_M98_V0.7040.75
97_G159_L0.7040.75
163_T166_L0.7000.74
256_G259_W0.6990.74
96_M162_Y0.6980.74
220_M225_V0.6970.74
40_R146_R0.6970.74
258_F263_F0.6960.74
273_I277_L0.6920.73
263_F275_P0.6910.73
270_L293_A0.6910.73
32_V36_V0.6880.73
94_M97_G0.6860.73
58_I65_I0.6860.73
250_L254_L0.6840.72
81_F84_L0.6810.72
225_V232_L0.6720.71
167_L310_L0.6720.71
17_P97_G0.6680.71
30_G159_L0.6670.71
154_V198_L0.6670.71
203_I212_T0.6650.70
92_S182_P0.6650.70
279_K294_A0.6640.70
19_A23_F0.6620.70
29_A185_F0.6590.70
261_W264_W0.6590.70
68_L105_M0.6590.70
37_L190_I0.6590.70
18_I26_G0.6560.69
231_L235_F0.6520.69
15_D102_Q0.6500.69
14_W94_M0.6500.69
275_P298_S0.6450.68
198_L295_C0.6450.68
120_L174_P0.6440.68
198_L291_V0.6430.68
200_A246_K0.6420.68
304_M308_F0.6380.67
221_E225_V0.6370.67
41_F293_A0.6340.67
174_P177_N0.6320.66
198_L202_A0.6290.66
179_P238_G0.6280.66
18_I74_H0.6250.66
221_E228_E0.6210.65
93_V169_A0.6200.65
220_M278_L0.6180.65
31_L104_Y0.6180.65
42_Y45_A0.6170.65
245_G249_A0.6170.65
114_K117_E0.6170.65
45_A49_D0.6150.64
94_M112_L0.6150.64
26_G29_A0.6140.64
200_A213_E0.6120.64
190_I194_A0.6110.64
37_L105_M0.6100.64
15_D76_T0.6090.64
184_L306_R0.6080.63
69_L164_G0.6070.63
248_R252_A0.6060.63
36_V113_A0.6030.63
230_F234_A0.6030.63
22_L310_L0.6010.63
10_E76_T0.5970.62
32_V183_V0.5960.62
267_V271_G0.5950.62
113_A118_H0.5940.62
21_Y163_T0.5910.61
247_V250_L0.5880.61
24_L66_L0.5870.61
53_L115_I0.5870.61
168_S182_P0.5860.61
274_V289_P0.5840.60
107_V110_L0.5840.60
21_Y25_I0.5830.60
199_I234_A0.5810.60
272_L275_P0.5800.60
34_L183_V0.5800.60
28_S165_F0.5800.60
232_L299_L0.5740.59
190_I202_A0.5730.59
162_Y166_L0.5730.59
26_G166_L0.5720.59
204_R212_T0.5700.58
10_E16_W0.5700.58
64_V186_L0.5700.58
187_F231_L0.5700.58
184_L191_S0.5700.58
86_F109_V0.5680.58
73_F298_S0.5670.58
236_F240_A0.5670.58
183_V231_L0.5660.58
75_L84_L0.5640.58
61_P303_L0.5640.58
277_L283_N0.5580.57
247_V254_L0.5580.57
24_L70_I0.5570.57
94_M162_Y0.5560.57
209_P227_G0.5550.56
98_V102_Q0.5500.56
218_H285_S0.5500.56
156_A160_G0.5490.56
32_V60_G0.5490.56
68_L102_Q0.5480.56
28_S192_S0.5480.56
66_L230_F0.5460.55
192_S305_L0.5460.55
226_W259_W0.5430.55
263_F301_G0.5410.55
164_G169_A0.5410.55
198_L214_A0.5410.55
59_V298_S0.5400.54
22_L26_G0.5400.54
26_G101_F0.5390.54
270_L275_P0.5380.54
68_L166_L0.5350.54
272_L276_M0.5340.54
101_F159_L0.5300.53
280_P286_S0.5300.53
240_A246_K0.5270.53
274_V284_R0.5270.53
299_L303_L0.5230.52
74_H81_F0.5230.52
274_V282_V0.5230.52
24_L71_L0.5230.52
24_L29_A0.5210.52
201_M204_R0.5200.52
42_Y151_L0.5190.52
35_A96_M0.5180.51
259_W263_F0.5170.51
86_F89_S0.5130.51
71_L101_F0.5120.51
283_N286_S0.5110.51
269_G272_L0.5080.50
177_N182_P0.5080.50
27_I193_G0.5070.50
167_L309_I0.5050.50
181_L302_V0.5050.50
93_V98_V0.5040.50
196_V292_L0.5030.49
38_L58_I0.5030.49
30_G92_S0.5030.49
211_S278_L0.5020.49
29_A228_E0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2vpzC 2 0.7767 100 0.395 Contact Map
4c7rA 3 0.9308 40.2 0.945 Contact Map
3s8fA 1 0.5503 13.6 0.956 Contact Map
4o6yA 2 0.5597 13 0.956 Contact Map
2wswA 3 0.934 11.5 0.957 Contact Map
3rkoA 1 0.2925 9.2 0.959 Contact Map
1qleC 1 0.7296 8.4 0.96 Contact Map
1m56C 2 0.7013 7.4 0.961 Contact Map
4us3A 1 0.7484 6.4 0.962 Contact Map
1v54C 1 0.6981 4.8 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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