GREMLIN Database
PFLC - Pyruvate formate-lyase 2-activating enzyme
UniProt: P32675 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11911
Length: 292 (263)
Sequences: 1100 (906)
Seq/√Len: 55.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
181_A191_N4.7241.00
123_L135_A4.6451.00
121_V142_L3.7751.00
155_G174_F3.2711.00
139_L149_C3.0791.00
174_F199_L3.0641.00
159_A201_L3.0151.00
278_R282_E2.9551.00
107_V142_L2.9471.00
148_S171_E2.9191.00
213_L241_I2.8141.00
181_A193_P2.7171.00
141_R144_L2.6361.00
106_E109_K2.5271.00
184_A192_L2.5051.00
77_A80_L2.4551.00
100_L141_R2.4431.00
108_M145_W2.4401.00
183_Q186_D2.4041.00
136_T164_P2.3861.00
25_F59_S2.3331.00
140_Q168_L2.3281.00
103_L138_F2.3051.00
98_I103_L2.2881.00
143_R170_D2.2381.00
185_R191_N2.0961.00
104_E142_L2.0951.00
187_V219_F2.0871.00
24_I44_F2.0861.00
152_E210_I2.0741.00
163_L201_L2.0671.00
101_D141_R1.9741.00
159_A197_E1.9661.00
225_N229_A1.9111.00
28_Q31_S1.9081.00
269_P274_V1.8931.00
211_P233_L1.8731.00
179_M232_V1.8660.99
73_C76_C1.8450.99
129_L157_A1.8150.99
230_L281_A1.7490.99
73_C86_C1.7480.99
220_T225_N1.7480.99
223_R276_T1.7180.99
159_A163_L1.7080.99
169_C172_V1.6490.99
86_C90_A1.6470.99
123_L138_F1.6370.99
123_L139_L1.6270.99
141_R145_W1.6260.99
73_C79_C1.6020.98
213_L229_A1.6010.98
76_C79_C1.6010.98
151_I172_V1.6000.98
210_I240_Q1.5830.98
231_D284_A1.5600.98
184_A190_M1.5290.98
79_C86_C1.5230.98
129_L165_L1.5190.98
179_M225_N1.4970.98
128_V135_A1.4940.98
128_V151_I1.4880.97
196_L232_V1.4760.97
143_R168_L1.4560.97
155_G199_L1.4540.97
223_R227_Q1.4460.97
156_D190_M1.4170.97
172_V202_L1.3980.96
111_D115_R1.3940.96
200_R232_V1.3850.96
203_V209_V1.3630.96
49_H195_V1.3630.96
96_R102_A1.3580.96
108_M111_D1.3370.95
199_L232_V1.3330.95
215_L243_L1.3200.95
129_L162_L1.3160.95
60_I259_K1.2980.94
133_E164_P1.2940.94
73_C90_A1.2870.94
280_M284_A1.2740.94
197_E201_L1.2680.94
178_I219_F1.2360.93
76_C86_C1.2180.92
108_M147_V1.2110.92
136_T165_L1.1980.92
65_Q259_K1.1970.92
269_P273_D1.1900.91
103_L107_V1.1860.91
143_R149_C1.1810.91
172_V207_V1.1810.91
179_M228_Q1.1790.91
55_A152_E1.1740.91
139_L169_C1.1650.90
279_E283_R1.1630.90
101_D105_R1.1590.90
183_Q222_S1.1530.90
129_L154_A1.1470.90
173_L242_H1.1460.90
217_P266_V1.1400.89
178_I220_T1.1360.89
104_E141_R1.1310.89
215_L274_V1.1190.89
129_L151_I1.1010.88
230_L284_A1.0950.87
69_R72_K1.0940.87
42_V107_V1.0900.87
123_L128_V1.0850.87
224_E228_Q1.0830.87
99_S102_A1.0780.87
136_T140_Q1.0700.86
274_V277_M1.0680.86
178_I225_N1.0650.86
200_R204_S1.0640.86
270_S273_D1.0620.86
210_I242_H1.0570.86
250_G261_W1.0420.85
100_L134_F1.0420.85
240_Q287_Q1.0420.85
111_D145_W1.0400.85
176_L199_L1.0330.84
79_C90_A1.0260.84
172_V199_L1.0250.84
247_H250_G1.0150.83
110_D114_F1.0130.83
156_D194_R1.0110.83
35_G39_R1.0010.82
40_T110_D0.9880.81
28_Q41_V0.9820.81
241_I281_A0.9760.81
200_R235_P0.9750.81
201_L205_E0.9750.81
176_L196_L0.9720.80
156_D191_N0.9720.80
53_W58_E0.9690.80
132_A161_K0.9690.80
47_C51_C0.9630.80
275_A278_R0.9600.79
197_E204_S0.9580.79
192_L196_L0.9550.79
142_L149_C0.9360.78
197_E200_R0.9350.78
203_V236_L0.9320.77
226_M281_A0.9310.77
76_C90_A0.9250.77
220_T247_H0.9200.77
48_P154_A0.9180.76
190_M195_V0.9170.76
215_L221_L0.9150.76
254_Y261_W0.9130.76
226_M277_M0.9110.76
152_E173_L0.9040.75
184_A188_V0.8960.75
29_R110_D0.8900.74
152_E249_Y0.8870.74
183_Q225_N0.8850.74
34_D39_R0.8750.73
135_A139_L0.8710.72
184_A195_V0.8680.72
186_D219_F0.8650.72
223_R273_D0.8620.72
152_E174_F0.8610.72
193_P197_E0.8570.71
50_L60_I0.8560.71
180_D228_Q0.8550.71
28_Q55_A0.8520.71
39_R57_P0.8520.71
35_G57_P0.8510.71
243_L277_M0.8480.70
107_V147_V0.8480.70
34_D43_F0.8470.70
121_V147_V0.8450.70
273_D277_M0.8400.70
158_P194_R0.8350.69
104_E145_W0.8330.69
178_I187_V0.8320.69
208_N239_R0.8290.69
29_R109_K0.8240.68
48_P130_M0.8210.68
136_T168_L0.8210.68
134_F217_P0.8180.68
39_R120_G0.8150.67
227_Q231_D0.8140.67
230_L286_L0.8140.67
173_L245_P0.8100.67
129_L135_A0.8070.67
179_M229_A0.8030.66
74_L77_A0.8010.66
36_E171_E0.8010.66
260_T264_K0.7990.66
24_I98_I0.7980.66
53_W57_P0.7980.66
166_A207_V0.7950.65
104_E108_M0.7930.65
69_R80_L0.7930.65
221_L273_D0.7930.65
39_R43_F0.7870.65
178_I183_Q0.7860.65
215_L219_F0.7760.64
202_L205_E0.7680.63
275_A279_E0.7650.62
45_K65_Q0.7640.62
75_H80_L0.7630.62
201_L204_S0.7590.62
182_T186_D0.7580.62
153_T174_F0.7550.61
100_L137_R0.7540.61
27_I42_V0.7540.61
26_N31_S0.7540.61
69_R75_H0.7520.61
49_H156_D0.7500.61
273_D276_T0.7490.61
164_P167_K0.7480.61
215_L277_M0.7470.61
279_E282_E0.7460.61
199_L211_P0.7420.60
163_L167_K0.7370.60
178_I188_V0.7340.59
128_V250_G0.7310.59
158_P161_K0.7280.59
134_F184_A0.7240.58
166_A201_L0.7220.58
26_N55_A0.7210.58
100_L138_F0.7180.58
156_D195_V0.7170.57
248_Q263_M0.7140.57
45_K95_G0.7140.57
277_M280_M0.7110.57
130_M154_A0.7110.57
34_D57_P0.7090.57
29_R114_F0.7090.57
54_C58_E0.7020.56
218_G287_Q0.6970.55
222_S225_N0.6940.55
228_Q231_D0.6910.55
155_G173_L0.6890.54
179_M196_L0.6890.54
155_G162_L0.6870.54
70_E80_L0.6850.54
246_F261_W0.6850.54
252_P256_L0.6840.54
25_F30_Y0.6840.54
152_E256_L0.6770.53
151_I157_A0.6710.53
70_E75_H0.6700.52
239_R287_Q0.6690.52
55_A60_I0.6640.52
39_R53_W0.6620.52
182_T185_R0.6610.51
78_K84_D0.6600.51
52_P188_V0.6580.51
230_L280_M0.6580.51
122_T212_R0.6560.51
103_L132_A0.6550.51
140_Q144_L0.6530.51
233_L241_I0.6490.50
107_V138_F0.6480.50
157_A162_L0.6460.50
55_A59_S0.6450.50
152_E242_H0.6430.50
39_R122_T0.6410.49
200_R236_L0.6410.49
69_R77_A0.6370.49
102_A138_F0.6320.48
45_K53_W0.6260.48
246_F263_M0.6220.47
152_E169_C0.6200.47
31_S41_V0.6190.47
51_C54_C0.6180.47
44_F128_V0.6170.47
233_L286_L0.6170.47
217_P221_L0.6170.47
66_T153_T0.6160.47
182_T224_E0.6140.46
199_L209_V0.6140.46
243_L269_P0.6140.46
227_Q284_A0.6120.46
248_Q251_E0.6090.46
155_G245_P0.6080.46
218_G221_L0.6070.46
111_D147_V0.6040.45
25_F28_Q0.6020.45
172_V209_V0.6020.45
47_C54_C0.6010.45
92_E256_L0.6000.45
54_C59_S0.5990.45
120_G212_R0.5980.45
231_D235_P0.5950.44
40_T58_E0.5940.44
226_M229_A0.5940.44
126_G212_R0.5940.44
29_R45_K0.5920.44
35_G58_E0.5910.44
56_N253_K0.5900.44
164_P182_T0.5870.43
286_L289_T0.5850.43
52_P187_V0.5850.43
203_V237_N0.5840.43
41_V152_E0.5810.43
254_Y257_L0.5810.43
94_I247_H0.5800.43
39_R45_K0.5790.43
35_G43_F0.5790.43
157_A165_L0.5780.43
42_V121_V0.5780.43
227_Q280_M0.5780.43
94_I256_L0.5750.42
143_R231_D0.5750.42
53_W214_P0.5730.42
105_R231_D0.5720.42
199_L202_L0.5700.42
38_I45_K0.5690.42
70_E77_A0.5660.41
162_L268_A0.5650.41
128_V157_A0.5650.41
185_R275_A0.5640.41
127_E154_A0.5640.41
44_F103_L0.5630.41
53_W59_S0.5620.41
107_V176_L0.5620.41
35_G59_S0.5600.41
87_P256_L0.5590.41
249_Y256_L0.5590.41
160_S163_L0.5580.40
43_F57_P0.5580.40
188_V192_L0.5580.40
66_T73_C0.5560.40
84_D204_S0.5540.40
276_T280_M0.5540.40
282_E287_Q0.5520.40
242_H256_L0.5510.40
33_N38_I0.5500.40
28_Q38_I0.5500.40
277_M281_A0.5490.39
213_L225_N0.5480.39
32_L281_A0.5460.39
246_F264_K0.5450.39
215_L220_T0.5450.39
196_L220_T0.5440.39
40_T53_W0.5440.39
184_A196_L0.5430.39
150_A171_E0.5420.39
28_Q33_N0.5410.39
96_R106_E0.5410.39
233_L236_L0.5410.39
143_R147_V0.5400.39
192_L195_V0.5380.38
34_D212_R0.5380.38
35_G44_F0.5380.38
78_K85_E0.5370.38
122_T126_G0.5370.38
145_W218_G0.5340.38
181_A185_R0.5340.38
103_L134_F0.5340.38
100_L143_R0.5310.38
263_M266_V0.5310.38
74_L80_L0.5280.37
203_V238_I0.5270.37
172_V208_N0.5240.37
126_G152_E0.5210.37
25_F33_N0.5210.37
152_E261_W0.5210.37
240_Q289_T0.5200.37
26_N32_L0.5200.37
243_L274_V0.5190.36
35_G126_G0.5190.36
51_C56_N0.5180.36
74_L88_S0.5180.36
92_E113_F0.5180.36
105_R283_R0.5160.36
104_E147_V0.5160.36
242_H289_T0.5150.36
121_V138_F0.5150.36
95_G110_D0.5130.36
241_I245_P0.5130.36
35_G53_W0.5130.36
251_E256_L0.5120.36
38_I49_H0.5090.35
28_Q32_L0.5090.35
89_G139_L0.5080.35
137_R140_Q0.5070.35
26_N45_K0.5070.35
118_G243_L0.5070.35
195_V246_F0.5070.35
100_L103_L0.5070.35
35_G45_K0.5060.35
28_Q253_K0.5040.35
103_L142_L0.5040.35
140_Q166_A0.5040.35
234_I278_R0.5030.35
30_Y33_N0.5030.35
39_R59_S0.5020.35
217_P263_M0.5010.35
160_S287_Q0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3c8fA 1 0.8288 100 0.588 Contact Map
3canA 2 0.5377 100 0.674 Contact Map
3rfaA 1 0.8253 100 0.73 Contact Map
2yx0A 1 0.8493 100 0.748 Contact Map
2z2uA 1 0.7603 100 0.75 Contact Map
1hfeL 2 0.8527 99.9 0.765 Contact Map
1oltA 1 0.8596 99.9 0.777 Contact Map
4njhA 2 0.6438 99.9 0.783 Contact Map
4u0oB 1 0.8116 99.9 0.784 Contact Map
1tv8A 2 0.7637 99.9 0.787 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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