GREMLIN Database
PTFLB - Fructose-like PTS system EIIBC component
UniProt: P32154 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11863
Length: 483 (450)
Sequences: 1491 (789)
Seq/√Len: 37.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
8_V63_V5.5511.00
356_A456_N5.2071.00
360_A368_F4.2311.00
212_V236_L4.0681.00
34_L101_S4.0121.00
48_V51_R3.9411.00
95_N98_Q3.7991.00
361_T376_A3.7971.00
348_V449_L3.7821.00
201_A205_A3.7681.00
189_I233_A3.7371.00
55_E59_A3.7251.00
9_A40_V3.6931.00
34_L97_D3.6461.00
62_Y103_L3.6301.00
362_F380_I3.6241.00
6_R59_A3.6181.00
193_G236_L3.5781.00
317_F323_I3.3621.00
243_F272_V3.3261.00
97_D101_S3.2921.00
31_A38_I3.2521.00
39_K56_E3.2181.00
423_K440_S3.1741.00
131_H199_S3.1031.00
317_F407_T3.0141.00
33_S97_D2.9861.00
313_A407_T2.9751.00
54_S58_A2.9141.00
101_S105_T2.8211.00
408_L459_F2.7881.00
91_Q95_N2.6651.00
142_F210_F2.6411.00
187_F214_Y2.5521.00
30_K33_S2.4681.00
398_A401_P2.4151.00
444_S447_L2.3911.00
28_E32_R2.3381.00
364_A461_I2.3081.00
6_R61_D2.2390.99
416_L420_F2.2240.99
27_L38_I2.1990.99
316_A410_G2.1140.99
80_A111_A2.0530.99
153_A178_E2.0470.99
357_A460_I2.0280.99
412_I455_L1.9950.99
451_I455_L1.9860.98
329_F332_F1.9710.98
244_V269_T1.9670.98
36_H100_F1.9580.98
39_K50_N1.9320.98
368_F461_I1.9070.98
138_A195_Y1.8980.98
331_C346_V1.8980.98
245_F249_K1.8940.98
348_V413_T1.8830.98
138_A206_F1.8750.98
25_E28_E1.8620.98
195_Y198_S1.8490.98
366_K466_L1.8430.98
6_R37_T1.8340.97
184_G211_L1.8240.97
80_A112_A1.8100.97
313_A317_F1.7930.97
447_L451_I1.7670.97
102_E106_N1.7670.97
268_V272_V1.7650.97
30_K34_L1.7410.96
355_V453_L1.7260.96
288_L291_G1.7230.96
311_V346_V1.7010.96
178_E182_Y1.6750.96
52_L57_I1.6720.96
8_V52_L1.6490.95
175_W179_S1.6410.95
207_A270_L1.6390.95
186_T214_Y1.6150.95
420_F444_S1.6040.94
340_Y425_L1.5880.94
150_V185_F1.5770.94
316_A353_P1.5730.94
319_M388_T1.5590.94
374_E377_S1.5590.94
134_A198_S1.5260.93
408_L451_I1.5170.93
194_A210_F1.4950.92
422_I444_S1.4920.92
309_F415_V1.4810.92
62_Y85_Y1.4700.91
197_A240_I1.4400.90
145_G270_L1.4360.90
34_L100_F1.4270.90
5_L61_D1.4240.90
316_A388_T1.4240.90
405_A459_F1.4220.90
191_I210_F1.4070.89
158_Q161_L1.4050.89
358_G362_F1.3930.89
30_K97_D1.3870.89
10_I43_Q1.3800.88
367_L466_L1.3780.88
290_G294_H1.3760.88
286_S290_G1.3740.88
313_A349_V1.3660.88
98_Q102_E1.3640.88
402_M460_I1.3490.87
358_G380_I1.3470.87
235_V239_A1.3470.87
244_V265_I1.3420.87
231_L235_V1.3310.87
9_A38_I1.3240.86
17_I258_P1.3180.86
291_G295_F1.3070.86
209_A240_I1.3040.85
212_V273_F1.3020.85
359_L453_L1.2970.85
9_A67_T1.2880.85
367_L461_I1.2860.85
176_V279_Y1.2730.84
42_T50_N1.2730.84
313_A411_G1.2560.83
412_I451_I1.2540.83
30_K96_I1.2510.83
45_S203_K1.2440.83
328_W343_Y1.2430.83
7_I31_A1.2430.83
385_T432_F1.2390.82
5_L104_P1.2320.82
24_A28_E1.2300.82
287_D291_G1.2130.81
403_I407_T1.2100.81
350_A386_V1.2070.81
265_I269_T1.2040.80
89_I92_A1.2000.80
180_I274_G1.2000.80
29_Q32_R1.1990.80
398_A464_K1.1980.80
87_I91_Q1.1970.80
161_L174_T1.1940.80
41_E78_R1.1930.80
441_P444_S1.1880.80
12_N69_R1.1860.79
405_A463_L1.1800.79
370_R373_K1.1790.79
302_S305_F1.1650.78
83_K109_L1.1630.78
458_S462_V1.1610.78
76_R86_E1.1570.78
7_I36_H1.1470.77
267_F271_L1.1470.77
208_P270_L1.1460.77
92_A95_N1.1440.77
360_A461_I1.1430.77
7_I104_P1.1350.76
422_I441_P1.1320.76
347_G386_V1.1280.76
299_I303_M1.1250.76
417_V422_I1.1220.76
416_L419_A1.1160.75
293_L297_N1.1150.75
328_W335_L1.1130.75
347_G350_A1.1100.75
457_I461_I1.1080.75
96_I100_F1.0970.74
10_I76_R1.0960.74
157_V168_K1.0920.73
124_Q127_S1.0860.73
448_V451_I1.0820.73
29_Q371_Q1.0800.73
53_S56_E1.0770.72
285_M326_T1.0660.72
102_E105_T1.0610.71
243_F273_F1.0600.71
91_Q102_E1.0600.71
352_M410_G1.0520.71
364_A367_L1.0520.71
45_S262_S1.0510.71
246_W250_V1.0490.70
416_L451_I1.0480.70
55_E58_A1.0470.70
247_F265_I1.0450.70
351_L386_V1.0450.70
87_I99_I1.0440.70
416_L448_V1.0430.70
173_F271_L1.0350.69
197_A209_A1.0340.69
200_I242_Y1.0310.69
127_S131_H1.0280.69
315_L347_G1.0270.69
291_G294_H1.0250.69
370_R374_E1.0230.68
76_R110_F1.0210.68
107_S110_F1.0200.68
285_M288_L1.0190.68
404_T463_L1.0170.68
289_M326_T1.0170.68
195_Y206_F1.0150.68
239_A243_F1.0140.68
299_I310_L1.0090.67
26_A30_K1.0080.67
183_L215_L1.0070.67
176_V275_V1.0050.67
92_A98_Q1.0040.67
122_E126_G1.0000.67
210_F214_Y0.9960.66
454_A457_I0.9940.66
95_N102_E0.9810.65
257_Q260_L0.9770.65
416_L444_S0.9760.65
422_I440_S0.9750.65
62_Y104_P0.9740.65
415_V419_A0.9680.64
315_L386_V0.9680.64
246_W249_K0.9670.64
228_A232_G0.9630.64
438_L442_V0.9610.64
454_A458_S0.9550.63
62_Y106_N0.9540.63
26_A93_L0.9530.63
353_P410_G0.9520.63
323_I407_T0.9450.62
140_L260_L0.9440.62
315_L350_A0.9420.62
67_T86_E0.9340.61
311_V342_W0.9320.61
252_L260_L0.9230.60
248_R388_T0.9230.60
357_A379_A0.9210.60
51_R75_D0.9160.60
209_A213_C0.9160.60
156_L173_F0.9130.60
65_L76_R0.9120.59
453_L457_I0.9110.59
29_Q33_S0.9080.59
157_V174_T0.9080.59
148_I267_F0.9060.59
367_L467_W0.9050.59
357_A388_T0.9050.59
411_G415_V0.9040.59
65_L84_V0.9030.59
43_Q65_L0.9030.59
193_G233_A0.9010.59
134_A138_A0.8990.58
124_Q128_V0.8970.58
153_A158_Q0.8930.58
276_L280_V0.8900.58
85_Y99_I0.8890.58
332_F343_Y0.8880.57
319_M406_N0.8830.57
10_I67_T0.8830.57
247_F269_T0.8820.57
58_A61_D0.8820.57
34_L96_I0.8810.57
459_F463_L0.8810.57
159_F162_P0.8790.57
90_S94_K0.8770.56
5_L37_T0.8770.56
292_L295_F0.8740.56
247_F250_V0.8720.56
328_W351_L0.8680.56
202_D248_R0.8680.56
305_F419_A0.8670.56
62_Y96_I0.8630.55
404_T407_T0.8580.55
363_I453_L0.8560.55
347_G351_L0.8550.55
352_M413_T0.8520.54
83_K107_S0.8510.54
244_V247_F0.8510.54
45_S261_G0.8470.54
304_K421_G0.8440.54
120_K123_V0.8410.53
162_P166_M0.8380.53
7_I62_Y0.8380.53
351_L432_F0.8370.53
408_L412_I0.8350.53
197_A205_A0.8330.53
129_M133_M0.8320.53
343_Y346_V0.8300.52
160_G174_T0.8290.52
424_R445_F0.8290.52
309_F411_G0.8280.52
187_F210_F0.8260.52
43_Q75_D0.8240.52
100_F104_P0.8240.52
288_L323_I0.8230.52
165_D170_A0.8220.52
57_I82_K0.8200.51
144_I375_A0.8190.51
406_N410_G0.8170.51
336_E412_I0.8170.51
153_A160_G0.8160.51
386_V414_G0.8160.51
5_L59_A0.8130.51
344_A347_G0.8120.51
7_I103_L0.8120.51
345_I418_I0.8100.51
260_L264_L0.8080.50
114_S118_L0.8080.50
247_F264_L0.8080.50
295_F310_L0.8070.50
346_V417_V0.8060.50
369_T397_A0.8060.50
131_H200_I0.8050.50
315_L351_L0.8050.50
227_G286_S0.8040.50
9_A36_H0.8020.50
312_G445_F0.8010.50
359_L454_A0.7980.50
85_Y91_Q0.7940.49
308_A312_G0.7920.49
409_A456_N0.7920.49
205_A243_F0.7890.49
445_F449_L0.7880.49
8_V56_E0.7880.49
10_I65_L0.7870.49
367_L465_G0.7830.48
349_V407_T0.7820.48
93_L99_I0.7820.48
43_Q78_R0.7810.48
96_I104_P0.7790.48
258_P358_G0.7780.48
215_L365_P0.7770.48
62_Y83_K0.7740.47
312_G349_V0.7720.47
371_Q374_E0.7680.47
333_S336_E0.7670.47
215_L282_G0.7660.47
406_N456_N0.7640.47
144_I262_S0.7630.46
252_L264_L0.7620.46
156_L170_A0.7590.46
284_V394_Y0.7570.46
302_S306_A0.7560.46
87_I95_N0.7550.46
156_L177_V0.7520.45
271_L275_V0.7510.45
21_Y397_A0.7510.45
63_V84_V0.7510.45
156_L160_G0.7490.45
211_L270_L0.7490.45
256_L385_T0.7460.45
43_Q76_R0.7440.45
309_F312_G0.7420.45
156_L161_L0.7410.44
124_Q242_Y0.7410.44
88_A94_K0.7410.44
152_L155_M0.7410.44
450_A454_A0.7390.44
253_G256_L0.7390.44
123_V126_G0.7380.44
295_F298_T0.7380.44
7_I38_I0.7350.44
364_A465_G0.7350.44
12_N67_T0.7340.44
248_R281_I0.7330.44
128_V238_L0.7330.44
235_V268_V0.7330.44
341_D421_G0.7310.44
183_L186_T0.7300.44
365_P373_K0.7300.44
89_I96_I0.7290.43
359_L457_I0.7290.43
332_F336_E0.7290.43
341_D417_V0.7290.43
77_A112_A0.7280.43
127_S242_Y0.7240.43
6_R56_E0.7220.43
43_Q380_I0.7210.43
157_V170_A0.7200.43
89_I99_I0.7190.43
13_C16_G0.7170.42
162_P165_D0.7160.42
443_G447_L0.7110.42
61_D82_K0.7090.42
351_L414_G0.7080.42
16_G19_H0.7070.42
158_Q174_T0.7070.42
45_S208_P0.7070.42
16_G20_T0.7060.41
457_I467_W0.7050.41
269_T273_F0.7040.41
67_T238_L0.7020.41
54_S458_S0.7000.41
13_C19_H0.7000.41
13_C20_T0.6990.41
7_I441_P0.6980.41
335_L343_Y0.6960.41
134_A206_F0.6960.41
413_T417_V0.6940.40
350_A385_T0.6940.40
285_M291_G0.6910.40
446_Y450_A0.6910.40
204_P248_R0.6900.40
359_L362_F0.6890.40
162_P170_A0.6880.40
161_L178_E0.6880.40
331_C382_V0.6880.40
208_P269_T0.6870.40
319_M354_P0.6870.40
173_F177_V0.6860.40
203_K261_G0.6840.40
309_F313_A0.6840.40
31_A34_L0.6830.40
328_W332_F0.6830.40
369_T372_E0.6820.39
35_G451_I0.6810.39
43_Q362_F0.6800.39
215_L278_Y0.6790.39
259_L264_L0.6780.39
62_Y100_F0.6770.39
412_I457_I0.6770.39
62_Y89_I0.6770.39
165_D168_K0.6760.39
30_K101_S0.6750.39
422_I438_L0.6740.39
401_P405_A0.6720.39
193_G212_V0.6710.38
328_W386_V0.6700.38
250_V268_V0.6670.38
28_E96_I0.6660.38
131_H242_Y0.6650.38
195_Y248_R0.6640.38
148_I211_L0.6630.38
155_M158_Q0.6620.38
175_W178_E0.6620.38
386_V432_F0.6620.38
425_L438_L0.6600.38
207_A269_T0.6590.37
359_L363_I0.6590.37
351_L431_I0.6590.37
28_E31_A0.6590.37
341_D418_I0.6550.37
52_L82_K0.6540.37
400_L404_T0.6540.37
333_S339_I0.6530.37
239_A273_F0.6500.37
85_Y210_F0.6490.37
417_V445_F0.6490.37
289_M379_A0.6470.37
317_F406_N0.6460.36
233_A273_F0.6460.36
432_F436_I0.6450.36
334_L339_I0.6420.36
158_Q170_A0.6410.36
286_S294_H0.6410.36
283_P286_S0.6390.36
7_I100_F0.6390.36
61_D104_P0.6380.36
344_A351_L0.6370.36
424_R432_F0.6360.36
85_Y106_N0.6360.36
448_V455_L0.6330.35
188_M233_A0.6330.35
443_G455_L0.6320.35
205_A244_V0.6310.35
138_A203_K0.6310.35
8_V39_K0.6300.35
201_A245_F0.6290.35
347_G432_F0.6280.35
45_S49_E0.6280.35
123_V127_S0.6270.35
292_L310_L0.6270.35
56_E59_A0.6240.35
363_I457_I0.6230.35
160_G170_A0.6220.34
303_M306_A0.6220.34
136_V143_V0.6210.34
343_Y428_G0.6210.34
281_I285_M0.6210.34
432_F438_L0.6180.34
344_A350_A0.6150.34
89_I248_R0.6150.34
39_K53_S0.6150.34
10_I75_D0.6140.34
43_Q52_L0.6130.34
157_V167_S0.6120.34
234_V333_S0.6110.34
193_G197_A0.6110.34
63_V82_K0.6060.33
144_I203_K0.6040.33
34_L58_A0.6040.33
106_N110_F0.6020.33
299_I453_L0.6020.33
155_M168_K0.6020.33
428_G433_D0.6010.33
312_G348_V0.6010.33
7_I107_S0.6010.33
91_Q96_I0.6000.33
132_L238_L0.6000.33
239_A272_V0.5970.33
317_F349_V0.5960.32
422_I448_V0.5960.32
263_M385_T0.5910.32
159_F210_F0.5910.32
335_L338_H0.5910.32
37_T59_A0.5910.32
355_V384_A0.5900.32
161_L170_A0.5890.32
49_E254_K0.5880.32
13_C18_A0.5880.32
228_A282_G0.5870.32
347_G431_I0.5870.32
330_F334_L0.5860.32
268_V271_L0.5850.32
436_I442_V0.5840.32
401_P464_K0.5840.32
259_L437_G0.5830.31
207_A334_L0.5830.31
87_I102_E0.5810.31
257_Q369_T0.5800.31
415_V418_I0.5800.31
344_A417_V0.5790.31
39_K63_V0.5790.31
200_I238_L0.5780.31
8_V339_I0.5780.31
155_M159_F0.5780.31
154_N350_A0.5780.31
176_V180_I0.5770.31
294_H297_N0.5760.31
356_A360_A0.5750.31
402_M406_N0.5740.31
150_V178_E0.5740.31
46_S49_E0.5740.31
442_V446_Y0.5730.31
258_P385_T0.5730.31
68_G89_I0.5720.31
152_L178_E0.5720.31
158_Q165_D0.5720.31
37_T264_L0.5720.31
153_A159_F0.5720.31
298_T301_P0.5710.31
272_V281_I0.5710.31
357_A406_N0.5710.31
36_H61_D0.5670.30
288_L295_F0.5660.30
136_V174_T0.5660.30
68_G349_V0.5650.30
25_E254_K0.5650.30
7_I92_A0.5650.30
276_L312_G0.5630.30
233_A342_W0.5630.30
114_S119_G0.5620.30
83_K106_N0.5610.30
234_V267_F0.5610.30
154_N386_V0.5600.30
122_E125_S0.5590.30
214_Y225_Q0.5590.30
259_L342_W0.5590.30
234_V415_V0.5580.30
345_I350_A0.5580.30
175_W335_L0.5570.29
62_Y99_I0.5560.29
85_Y102_E0.5550.29
284_V342_W0.5540.29
21_Y398_A0.5530.29
198_S206_F0.5510.29
406_N459_F0.5510.29
277_T344_A0.5500.29
97_D100_F0.5500.29
69_R319_M0.5490.29
115_G118_L0.5490.29
6_R55_E0.5480.29
348_V355_V0.5480.29
14_P67_T0.5470.29
188_M417_V0.5470.29
187_F342_W0.5470.29
348_V417_V0.5450.29
114_S121_Q0.5440.29
48_V202_D0.5430.28
154_N347_G0.5430.28
306_A310_L0.5410.28
284_V288_L0.5410.28
444_S451_I0.5400.28
157_V165_D0.5390.28
179_S182_Y0.5390.28
372_E460_I0.5390.28
85_Y107_S0.5390.28
335_L416_L0.5390.28
320_G324_N0.5380.28
267_F426_A0.5370.28
67_T99_I0.5370.28
436_I439_M0.5360.28
360_A456_N0.5360.28
233_A329_F0.5360.28
462_V466_L0.5360.28
328_W350_A0.5350.28
308_A415_V0.5350.28
71_L247_F0.5350.28
231_L410_G0.5350.28
99_I102_E0.5340.28
195_Y331_C0.5340.28
236_L277_T0.5340.28
140_L326_T0.5340.28
153_A165_D0.5330.28
352_M453_L0.5320.28
208_P261_G0.5320.28
157_V161_L0.5320.28
106_N109_L0.5320.28
113_D117_K0.5320.28
210_F269_T0.5310.28
77_A80_A0.5300.28
133_M138_A0.5300.28
296_L330_F0.5300.28
152_L156_L0.5280.27
188_M206_F0.5280.27
67_T71_L0.5270.27
294_H298_T0.5260.27
350_A414_G0.5260.27
363_I461_I0.5260.27
208_P378_S0.5260.27
14_P233_A0.5260.27
347_G424_R0.5250.27
103_L308_A0.5250.27
118_L121_Q0.5250.27
128_V400_L0.5250.27
350_A432_F0.5250.27
447_L450_A0.5240.27
153_A255_A0.5240.27
43_Q138_A0.5230.27
137_S327_A0.5230.27
233_A303_M0.5230.27
171_P175_W0.5230.27
9_A245_F0.5230.27
388_T410_G0.5210.27
328_W417_V0.5210.27
88_A91_Q0.5210.27
250_V272_V0.5210.27
331_C437_G0.5200.27
167_S170_A0.5200.27
158_Q162_P0.5190.27
328_W344_A0.5190.27
62_Y105_T0.5190.27
352_M357_A0.5170.27
92_A99_I0.5170.27
250_V403_I0.5160.27
25_E49_E0.5150.26
409_A452_G0.5150.26
408_L455_L0.5150.26
423_K448_V0.5150.26
363_I458_S0.5140.26
64_I85_Y0.5140.26
206_F407_T0.5140.26
165_D171_P0.5140.26
442_V450_A0.5130.26
191_I444_S0.5130.26
332_F335_L0.5130.26
356_A406_N0.5130.26
366_K467_W0.5130.26
14_P324_N0.5120.26
118_L122_E0.5110.26
201_A299_I0.5110.26
258_P328_W0.5110.26
48_V56_E0.5090.26
143_V154_N0.5090.26
304_K341_D0.5090.26
49_E140_L0.5090.26
352_M406_N0.5080.26
6_R38_I0.5060.26
43_Q69_R0.5060.26
354_P379_A0.5060.26
140_L144_I0.5060.26
88_A153_A0.5050.26
156_L176_V0.5050.26
334_L340_Y0.5050.26
55_E455_L0.5050.26
179_S306_A0.5050.26
315_L414_G0.5050.26
92_A185_F0.5030.26
386_V431_I0.5030.26
193_G213_C0.5020.26
157_V171_P0.5010.26
149_L177_V0.5000.25
231_L285_M0.5000.25
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2r48A 1 0.2174 100 0.786 Contact Map
3qnqA 2 0.7598 100 0.802 Contact Map
2r4qA 1 0.2112 100 0.828 Contact Map
2kyrA 1 0.2236 100 0.838 Contact Map
2m1zA 1 0.2195 100 0.846 Contact Map
4tn5A 2 0.234 100 0.85 Contact Map
4mgeA 3 0.2091 99.8 0.901 Contact Map
1e2bA 1 0.2153 99.7 0.909 Contact Map
2l2qA 1 0.2112 99.6 0.915 Contact Map
3czcA 1 0.1905 99 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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