GREMLIN Database
HEMN - Oxygen-independent coproporphyrinogen-III oxidase
UniProt: P32131 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11836
Length: 457 (376)
Sequences: 6241 (4469)
Seq/√Len: 230.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
90_E129_L4.0451.00
227_K276_F3.2131.00
223_A272_E2.9571.00
90_E125_R2.9331.00
87_D125_R2.8271.00
233_N292_R2.6851.00
199_R235_D2.6781.00
187_D190_F2.6681.00
94_V133_N2.6611.00
94_V129_L2.5191.00
417_D424_Q2.4601.00
426_T429_G2.4331.00
88_A347_Y2.4041.00
136_F140_A2.2521.00
167_L198_A2.2521.00
230_A280_S2.2121.00
83_D122_Q2.2111.00
153_L200_E2.1631.00
54_S312_G2.1481.00
84_Q351_D2.1131.00
364_T367_D2.1131.00
65_L73_K2.1061.00
124_S128_K2.1051.00
119_N122_Q2.0941.00
262_S265_Q2.0311.00
223_A276_F2.0191.00
192_F228_R2.0071.00
196_N200_E2.0071.00
385_Y418_V2.0051.00
175_N221_S1.9611.00
171_V191_I1.9451.00
222_F273_T1.9191.00
124_S161_A1.9171.00
410_L436_I1.9111.00
174_F229_V1.8781.00
57_V130_L1.8671.00
176_K188_E1.8641.00
93_I324_M1.8631.00
85_Y326_V1.8421.00
224_F228_R1.8201.00
86_L90_E1.8111.00
342_E345_Q1.8021.00
176_K189_E1.7991.00
83_D125_R1.7791.00
275_A279_Q1.7751.00
330_S337_A1.7611.00
144_I164_F1.7601.00
193_A196_N1.7561.00
55_L105_V1.7481.00
205_S295_D1.7341.00
408_A412_K1.7331.00
88_A351_D1.7291.00
230_A276_F1.7211.00
410_L439_C1.7151.00
348_Q352_E1.7051.00
239_V273_T1.7011.00
156_L198_A1.6931.00
228_R231_E1.6731.00
411_A418_V1.6661.00
220_E223_A1.6421.00
189_E193_A1.6381.00
59_I118_L1.6371.00
160_R203_F1.6351.00
93_I130_L1.6321.00
91_Q94_V1.6171.00
178_V221_S1.6161.00
276_F280_S1.6101.00
177_E221_S1.6051.00
208_I229_V1.5871.00
219_P272_E1.5831.00
192_F196_N1.5551.00
219_P268_D1.5531.00
223_A227_K1.5471.00
120_K158_H1.5391.00
92_E339_N1.5361.00
213_G266_K1.5331.00
89_L324_M1.5281.00
153_L197_H1.5251.00
272_E275_A1.5021.00
188_E192_F1.4951.00
157_D201_I1.4941.00
389_E395_H1.4921.00
384_D421_K1.4911.00
226_L276_F1.4871.00
384_D387_P1.4851.00
86_L126_L1.4801.00
385_Y422_G1.4751.00
224_F227_K1.4741.00
386_A421_K1.4741.00
369_I388_I1.4581.00
234_P282_Y1.4521.00
274_I290_F1.4481.00
190_F193_A1.4481.00
311_Q379_C1.4361.00
82_A119_N1.4291.00
227_K231_E1.4241.00
344_K348_Q1.4231.00
278_T427_A1.4231.00
340_Q349_Q1.4231.00
104_H137_N1.4161.00
338_Q359_R1.4121.00
175_N178_V1.4111.00
95_H350_V1.3941.00
219_P265_Q1.3911.00
329_I338_Q1.3901.00
408_A411_A1.3901.00
385_Y397_A1.3891.00
409_P439_C1.3891.00
380_N430_R1.3701.00
199_R233_N1.3671.00
315_T381_F1.3521.00
104_H135_Q1.3491.00
158_H162_E1.3471.00
425_V433_I1.3421.00
174_F228_R1.3401.00
108_L136_F1.3391.00
157_D161_A1.3271.00
335_C360_G1.3191.00
283_Q296_E1.3181.00
171_V174_F1.3171.00
373_V383_L1.3041.00
340_Q358_W1.2901.00
124_S162_E1.2891.00
149_R185_E1.2841.00
177_E216_K1.2821.00
178_V225_T1.2791.00
175_N224_F1.2731.00
188_E228_R1.2691.00
86_L122_Q1.2671.00
409_P413_D1.2641.00
177_E180_R1.2581.00
235_D282_Y1.2511.00
82_A122_Q1.2501.00
130_L136_F1.2461.00
237_L277_L1.2401.00
272_E276_F1.2371.00
283_Q293_P1.2211.00
118_L122_Q1.2181.00
95_H355_N1.2171.00
55_L324_M1.2101.00
276_F279_Q1.2081.00
271_Q431_L1.2031.00
193_A197_H1.2001.00
72_N327_S1.2001.00
372_D392_W1.2001.00
195_L232_L1.1991.00
235_D295_D1.1961.00
315_T379_C1.1941.00
343_L347_Y1.1931.00
206_T235_D1.1881.00
116_T159_L1.1831.00
182_V214_L1.1821.00
169_M198_A1.1791.00
106_S137_N1.1671.00
275_A427_A1.1651.00
156_L201_I1.1511.00
167_L203_F1.1491.00
340_Q345_Q1.1451.00
146_V151_I1.1421.00
59_I326_V1.1401.00
249_F253_R1.1351.00
199_R206_T1.1331.00
241_N266_K1.1321.00
218_T221_S1.1281.00
256_K259_D1.1241.00
176_K180_R1.1241.00
372_D391_Q1.1241.00
155_V159_L1.1191.00
205_S235_D1.1151.00
312_G319_T1.1101.00
413_D428_K1.1071.00
419_D424_Q1.1061.00
91_Q351_D1.1061.00
110_W126_L1.1031.00
350_V356_A1.0981.00
395_H398_D1.0971.00
86_L129_L1.0971.00
425_V436_I1.0961.00
197_H200_E1.0941.00
144_I159_L1.0921.00
55_L130_L1.0881.00
384_D422_G1.0861.00
92_E346_Y1.0851.00
265_Q268_D1.0821.00
128_K132_E1.0821.00
90_E94_V1.0821.00
173_D178_V1.0791.00
93_I133_N1.0751.00
129_L132_E1.0721.00
323_G328_A1.0711.00
153_L157_D1.0691.00
334_D368_C1.0671.00
86_L118_L1.0661.00
372_D376_S1.0641.00
120_K155_V1.0601.00
110_W123_I1.0591.00
361_I367_D1.0551.00
153_L201_I1.0501.00
96_R324_M1.0481.00
219_P223_A1.0451.00
181_L216_K1.0371.00
116_T146_V1.0361.00
94_V132_E1.0351.00
387_P391_Q1.0241.00
252_Q257_D1.0181.00
365_R368_C1.0151.00
326_V343_L1.0091.00
95_H356_A1.0051.00
72_N326_V1.0051.00
229_V237_L1.0041.00
320_D331_M1.0011.00
219_P269_I0.9961.00
67_Y249_F0.9911.00
382_R424_Q0.9891.00
195_L199_R0.9851.00
195_L206_T0.9831.00
103_R320_D0.9821.00
152_E155_V0.9821.00
369_I399_Y0.9791.00
413_D439_C0.9791.00
376_S380_N0.9781.00
154_D158_H0.9771.00
293_P296_E0.9761.00
277_L282_Y0.9751.00
198_A206_T0.9731.00
274_I284_F0.9731.00
122_Q125_R0.9691.00
419_D422_G0.9651.00
131_R136_F0.9621.00
216_K259_D0.9601.00
367_D370_R0.9601.00
296_E381_F0.9541.00
264_Q268_D0.9541.00
195_L234_P0.9501.00
158_H161_A0.9501.00
217_Q269_I0.9471.00
139_D308_R0.9441.00
335_C362_A0.9421.00
349_Q354_G0.9331.00
116_T144_I0.9331.00
174_F225_T0.9291.00
433_I437_C0.9251.00
106_S308_R0.9221.00
188_E191_I0.9201.00
53_L103_R0.9191.00
382_R422_G0.9171.00
127_M142_I0.9161.00
192_F232_L0.9121.00
83_D87_D0.9091.00
345_Q348_Q0.9081.00
137_N140_A0.9030.99
93_I129_L0.9030.99
131_R135_Q0.9020.99
407_L423_I0.9010.99
409_P412_K0.9000.99
274_I278_T0.8970.99
281_G293_P0.8950.99
123_I162_E0.8920.99
87_D90_E0.8890.99
123_I144_I0.8870.99
124_S158_H0.8850.99
417_D426_T0.8830.99
374_I437_C0.8810.99
169_M194_L0.8800.99
106_S312_G0.8780.99
385_Y421_K0.8770.99
165_N205_S0.8770.99
129_L133_N0.8760.99
146_V169_M0.8760.99
83_D121_A0.8750.99
418_V423_I0.8740.99
386_A390_K0.8690.99
160_R204_T0.8660.99
110_W144_I0.8650.99
143_S307_H0.8640.99
248_I251_A0.8630.99
154_D157_D0.8620.99
160_R201_I0.8590.99
376_S383_L0.8560.99
208_I234_P0.8520.99
199_R234_P0.8480.99
210_L225_T0.8460.99
315_T319_T0.8440.99
265_Q269_I0.8430.99
316_Q331_M0.8410.99
274_I427_A0.8390.99
93_I134_F0.8370.99
244_H253_R0.8340.99
127_M130_L0.8260.99
74_I343_L0.8260.99
63_H150_E0.8240.99
119_N155_V0.8230.99
397_A418_V0.8230.99
230_A277_L0.8190.99
250_A254_K0.8140.99
316_Q368_C0.8140.99
323_G330_S0.8130.99
105_V140_A0.8130.99
149_R190_F0.8060.99
86_L125_R0.8050.99
88_A91_Q0.8050.99
127_M162_E0.8040.99
204_T295_D0.7960.99
323_G337_A0.7950.99
52_P106_S0.7940.99
311_Q315_T0.7920.99
54_S107_Q0.7920.99
207_N307_H0.7890.99
128_K131_R0.7880.99
320_D332_I0.7880.99
316_Q372_D0.7850.99
345_Q349_Q0.7840.99
210_L229_V0.7830.99
385_Y423_I0.7830.99
195_L208_I0.7820.99
347_Y351_D0.7810.99
174_F188_E0.7790.98
370_R441_D0.7780.98
109_H307_H0.7770.98
244_H249_F0.7740.98
67_Y183_N0.7740.98
171_V208_I0.7730.98
366_D399_Y0.7730.98
120_K154_D0.7730.98
91_Q95_H0.7720.98
123_I127_M0.7710.98
268_D271_Q0.7700.98
81_K84_Q0.7680.98
309_N313_Y0.7680.98
249_F258_A0.7650.98
151_I156_L0.7620.98
108_L140_A0.7620.98
121_A124_S0.7590.98
363_L368_C0.7590.98
168_S207_N0.7590.98
85_Y89_L0.7580.98
89_L126_L0.7570.98
365_R369_I0.7570.98
407_L416_V0.7530.98
376_S387_P0.7530.98
267_L431_L0.7520.98
157_D200_E0.7520.98
210_L222_F0.7500.98
97_A133_N0.7490.98
127_M131_R0.7480.98
212_Y239_V0.7420.98
435_N438_M0.7410.98
401_A408_A0.7370.98
65_L75_V0.7360.98
84_Q347_Y0.7340.98
116_T150_E0.7300.98
88_A350_V0.7280.98
102_G135_Q0.7270.98
415_L432_L0.7240.98
67_Y182_V0.7210.97
400_F403_D0.7210.97
233_N282_Y0.7200.97
156_L167_L0.7160.97
357_L360_G0.7150.97
268_D272_E0.7150.97
194_L197_H0.7140.97
410_L416_V0.7120.97
274_I430_R0.7120.97
432_L435_N0.7110.97
90_E126_L0.7090.97
415_L429_G0.7090.97
51_R320_D0.7070.97
386_A389_E0.7050.97
76_T82_A0.7040.97
434_R437_C0.7040.97
428_K431_L0.7040.97
179_Q186_Q0.7020.97
95_H99_L0.7020.97
428_K432_L0.7020.97
350_V353_Q0.6990.97
372_D388_I0.6980.97
387_P390_K0.6970.97
251_A254_K0.6970.97
326_V346_Y0.6960.97
248_I252_Q0.6910.97
250_A253_R0.6880.97
353_Q356_A0.6870.97
96_R322_L0.6840.97
226_L230_A0.6840.97
136_F142_I0.6830.97
411_A416_V0.6820.96
376_S379_C0.6800.96
222_F226_L0.6780.96
388_I394_L0.6780.96
84_Q88_A0.6750.96
385_Y396_F0.6690.96
403_D439_C0.6680.96
366_D370_R0.6660.96
284_F430_R0.6660.96
370_R374_I0.6660.96
198_A203_F0.6650.96
63_H185_E0.6630.96
264_Q267_L0.6630.96
408_A418_V0.6600.96
121_A158_H0.6590.96
214_L217_Q0.6570.96
226_L272_E0.6570.96
139_D142_I0.6570.96
52_P104_H0.6550.96
388_I396_F0.6530.96
176_K187_D0.6520.95
169_M191_I0.6500.95
120_K162_E0.6500.95
57_V324_M0.6470.95
116_T155_V0.6460.95
246_P250_A0.6460.95
64_K183_N0.6450.95
191_I208_I0.6440.95
270_L284_F0.6430.95
142_I164_F0.6430.95
247_T250_A0.6420.95
90_E133_N0.6390.95
197_H201_I0.6380.95
307_H311_Q0.6350.95
98_P133_N0.6350.95
96_R99_L0.6350.95
178_V214_L0.6350.95
57_V89_L0.6340.95
373_V388_I0.6330.95
221_S225_T0.6330.95
130_L324_M0.6330.95
151_I197_H0.6310.95
389_E396_F0.6300.95
189_E192_F0.6280.94
397_A401_A0.6280.94
110_W127_M0.6280.94
400_F440_F0.6260.94
309_N314_T0.6250.94
220_E224_F0.6250.94
206_T234_P0.6240.94
267_L271_Q0.6220.94
336_Y371_R0.6210.94
55_L322_L0.6170.94
235_D292_R0.6170.94
223_A275_A0.6140.94
227_K280_S0.6140.94
394_L399_Y0.6080.93
80_H84_Q0.6070.93
396_F400_F0.6060.93
105_V134_F0.6040.93
151_I155_V0.6030.93
66_C69_C0.6030.93
105_V130_L0.6020.93
373_V440_F0.5990.93
72_N343_L0.5970.93
388_I399_Y0.5950.93
363_L367_D0.5910.93
348_Q351_D0.5900.92
86_L119_N0.5890.92
369_I392_W0.5880.92
93_I126_L0.5880.92
396_F399_Y0.5870.92
230_A233_N0.5870.92
291_A296_E0.5860.92
123_I158_H0.5840.92
151_I194_L0.5830.92
251_A255_I0.5810.92
64_K185_E0.5790.92
198_A201_I0.5760.92
372_D387_P0.5760.92
81_K343_L0.5740.91
207_N238_S0.5730.91
79_Q122_Q0.5720.91
88_A346_Y0.5710.91
110_W142_I0.5690.91
385_Y389_E0.5690.91
82_A86_L0.5680.91
403_D410_L0.5670.91
192_F231_E0.5670.91
289_H310_F0.5670.91
188_E232_L0.5660.91
123_I159_L0.5650.91
156_L203_F0.5590.90
173_D225_T0.5580.90
377_L423_I0.5580.90
327_S341_K0.5570.90
271_Q275_A0.5570.90
273_T276_F0.5570.90
308_R311_Q0.5570.90
95_H354_G0.5560.90
416_V423_I0.5550.90
95_H353_Q0.5540.90
270_L274_I0.5520.90
108_L142_I0.5520.90
283_Q380_N0.5510.90
214_L225_T0.5510.90
230_A282_Y0.5510.90
236_R285_I0.5510.90
435_N439_C0.5490.90
177_E181_L0.5480.89
130_L133_N0.5480.89
54_S106_S0.5480.89
156_L160_R0.5470.89
410_L413_D0.5460.89
53_L322_L0.5450.89
278_T283_Q0.5450.89
174_F191_I0.5440.89
106_S139_D0.5430.89
196_N199_R0.5410.89
292_R295_D0.5410.89
110_W164_F0.5400.89
123_I164_F0.5400.89
394_L398_D0.5400.89
59_I86_L0.5400.89
284_F290_F0.5390.89
109_H143_S0.5390.89
310_F314_T0.5380.89
57_V126_L0.5370.88
414_G428_K0.5370.88
307_H314_T0.5360.88
79_Q83_D0.5360.88
90_E132_E0.5360.88
371_R375_K0.5360.88
150_E185_E0.5360.88
378_I437_C0.5340.88
222_F269_I0.5340.88
226_L273_T0.5330.88
236_R378_I0.5330.88
62_C66_C0.5330.88
70_G288_D0.5300.88
79_Q82_A0.5290.88
285_I289_H0.5280.88
107_Q140_A0.5270.88
227_K230_A0.5270.88
245_L249_F0.5270.88
118_L123_I0.5260.87
338_Q341_K0.5250.87
96_R337_A0.5240.87
286_G309_N0.5240.87
125_R129_L0.5240.87
227_K279_Q0.5220.87
97_A134_F0.5220.87
67_Y248_I0.5220.87
70_G243_A0.5220.87
156_L197_H0.5210.87
84_Q87_D0.5210.87
71_C310_F0.5210.87
67_Y252_Q0.5200.87
407_L411_A0.5190.87
383_L423_I0.5170.87
104_H138_A0.5170.87
288_D310_F0.5170.87
156_L194_L0.5160.86
234_P237_L0.5160.86
186_Q191_I0.5140.86
365_R394_L0.5140.86
388_I392_W0.5120.86
284_F431_L0.5120.86
59_I89_L0.5110.86
287_M433_I0.5110.86
273_T277_L0.5100.86
236_R309_N0.5060.85
314_T321_L0.5060.85
425_V429_G0.5050.85
221_S224_F0.5040.85
286_G314_T0.5040.85
287_M309_N0.5040.85
82_A117_Y0.5000.85
285_I314_T0.5000.85
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1oltA 1 0.9497 100 0.299 Contact Map
1r30A 2 0.6368 99.9 0.795 Contact Map
4u0oB 1 0.547 99.9 0.796 Contact Map
2qgqA 1 0.5689 99.9 0.801 Contact Map
4r33A 2 0.744 99.9 0.804 Contact Map
4jc0A 1 0.6958 99.9 0.808 Contact Map
4wcxA 1 0.8621 99.9 0.81 Contact Map
2yx0A 1 0.593 99.9 0.818 Contact Map
4rtbA 2 0.8118 99.9 0.82 Contact Map
3t7vA 1 0.6761 99.9 0.822 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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