GREMLIN Database
WCAI - Putative colanic acid biosynthesis glycosyl transferase WcaI
UniProt: P32057 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11790
Length: 407 (344)
Sequences: 53233 (44708)
Seq/√Len: 2410.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
235_L305_G4.7761.00
262_I288_Q3.6931.00
272_K276_E3.3171.00
176_N199_I3.2031.00
308_H328_I3.1591.00
233_I264_A2.9481.00
255_R368_V2.7981.00
110_I405_I2.7921.00
264_A290_F2.7381.00
387_Y391_T2.7201.00
231_K262_I2.7031.00
251_E278_M2.6751.00
236_Y246_L2.6691.00
257_R286_N2.3271.00
185_M189_I2.2661.00
251_E364_V2.2471.00
232_K375_L2.2081.00
36_R106_P2.2071.00
245_G248_N2.1871.00
36_R104_W2.1791.00
377_L380_H2.1301.00
278_M282_R2.0771.00
370_G373_Q2.0691.00
339_A346_G2.0651.00
385_R389_E2.0601.00
233_I262_I2.0531.00
4_L106_P2.0521.00
271_G274_R2.0401.00
266_V301_L2.0351.00
264_A288_Q2.0121.00
33_H402_I1.9781.00
234_I307_C1.9671.00
232_K306_D1.9611.00
253_A263_F1.9571.00
255_R365_E1.9411.00
349_C356_A1.9411.00
233_I304_M1.8981.00
373_Q377_L1.8841.00
110_I136_H1.8811.00
350_E354_G1.8661.00
245_G313_K1.8251.00
357_V370_G1.8051.00
332_G385_R1.8051.00
396_N400_Q1.7811.00
355_I387_Y1.7631.00
31_Q399_R1.7531.00
182_R185_M1.7511.00
254_D282_R1.7491.00
360_E366_A1.7471.00
36_R68_W1.7471.00
185_M200_F1.7181.00
308_H333_G1.7091.00
373_Q380_H1.7091.00
293_Q297_A1.7011.00
249_V263_F1.6751.00
204_W394_K1.6631.00
135_L174_V1.6631.00
182_R347_Q1.6421.00
179_T198_V1.6321.00
237_S298_L1.6251.00
372_R376_L1.6081.00
350_E387_Y1.5971.00
357_V373_Q1.5921.00
253_A261_L1.5901.00
339_A360_E1.5891.00
27_W395_E1.5701.00
27_W398_L1.5551.00
176_N404_D1.5441.00
294_S297_A1.5381.00
363_S366_A1.5291.00
27_W30_A1.5241.00
185_M198_V1.5241.00
249_V309_L1.5121.00
366_A370_G1.5041.00
354_G383_V1.5021.00
182_R200_F1.5001.00
22_G26_E1.4991.00
381_N384_A1.4871.00
266_V290_F1.4721.00
243_K314_R1.4721.00
248_N364_V1.4721.00
337_I345_L1.4651.00
256_L372_R1.4641.00
297_A300_A1.4531.00
247_E278_M1.4461.00
248_N313_K1.4441.00
280_Q285_R1.4371.00
31_Q402_I1.4311.00
4_L38_I1.4301.00
36_R66_T1.4271.00
350_E355_I1.4071.00
359_V370_G1.4071.00
305_G333_G1.3861.00
179_T185_M1.3861.00
293_Q301_L1.3861.00
266_V293_Q1.3631.00
268_Q272_K1.3571.00
236_Y249_V1.3541.00
255_R364_V1.3431.00
252_A367_L1.3421.00
364_V368_V1.3391.00
264_A301_L1.3381.00
299_P331_V1.3371.00
277_K281_Q1.3361.00
233_I306_D1.3351.00
240_I265_I1.3191.00
235_L266_V1.3181.00
111_G133_T1.2841.00
189_I195_A1.2791.00
234_I371_I1.2741.00
278_M281_Q1.2731.00
366_A369_A1.2701.00
194_A197_N1.2661.00
234_I309_L1.2651.00
264_A305_G1.2641.00
396_N399_R1.2571.00
246_L265_I1.2551.00
178_S401_F1.2521.00
5_V112_V1.2521.00
5_V28_L1.2501.00
383_V386_E1.2431.00
400_Q403_N1.2371.00
182_R186_N1.2231.00
290_F293_Q1.2061.00
210_F331_V1.2041.00
317_A326_T1.1991.00
232_K307_C1.1991.00
368_V372_R1.1761.00
303_K331_V1.1691.00
273_A276_E1.1671.00
186_N189_I1.1501.00
20_Y319_A1.1321.00
298_L302_L1.1271.00
27_W31_Q1.1261.00
332_G382_T1.1241.00
247_E251_E1.1201.00
295_Y298_L1.1111.00
393_D396_N1.1111.00
273_A277_K1.1081.00
359_V367_L1.1051.00
232_K261_L1.1011.00
302_L331_V1.0991.00
338_T359_V1.0961.00
233_I305_G1.0941.00
394_K397_V1.0851.00
231_K260_P1.0831.00
276_E280_Q1.0811.00
179_T188_A1.0801.00
397_V400_Q1.0801.00
248_N338_T1.0781.00
209_R299_P1.0741.00
337_I358_C1.0721.00
180_I184_M1.0721.00
351_T370_G1.0691.00
5_V25_V1.0681.00
313_K362_E1.0651.00
248_N251_E1.0601.00
180_I203_N1.0551.00
178_S404_D1.0541.00
311_V367_L1.0521.00
235_L301_L1.0351.00
299_P303_K1.0351.00
386_E389_E1.0311.00
290_F301_L1.0291.00
232_K260_P1.0251.00
369_A373_Q1.0241.00
251_E282_R1.0191.00
237_S266_V1.0191.00
248_N362_E1.0191.00
344_E347_Q1.0171.00
333_G381_N1.0171.00
382_T386_E1.0051.00
23_E26_E1.0011.00
237_S302_L0.9991.00
23_E394_K0.9981.00
357_V374_A0.9931.00
312_Q315_G0.9891.00
279_A289_F0.9881.00
329_L335_A0.9831.00
239_N243_K0.9791.00
29_A32_G0.9751.00
254_D258_D0.9741.00
180_I316_A0.9711.00
315_G318_D0.9681.00
111_G135_L0.9681.00
381_N385_R0.9641.00
210_F299_P0.9641.00
247_E274_R0.9621.00
234_I253_A0.9611.00
264_A304_M0.9591.00
207_I393_D0.9581.00
250_I279_A0.9571.00
138_Q319_A0.9561.00
186_N190_E0.9541.00
204_W397_V0.9541.00
397_V401_F0.9501.00
248_N367_L0.9491.00
125_L129_S0.9461.00
256_L371_I0.9461.00
179_T184_M0.9451.00
249_V311_V0.9431.00
25_V37_V0.9391.00
328_I333_G0.9381.00
247_E313_K0.9291.00
15_T18_G0.9281.00
321_L345_L0.9281.00
20_Y136_H0.9231.00
236_Y265_I0.9221.00
19_K23_E0.9211.00
336_V359_V0.9201.00
254_D257_R0.9171.00
174_V177_V0.9161.00
337_I349_C0.9141.00
318_D345_L0.9131.00
279_A284_L0.9131.00
261_L375_L0.9111.00
308_H320_V0.9041.00
310_V320_V0.9041.00
253_A286_N0.9011.00
96_F128_L0.9001.00
21_T112_V0.8971.00
256_L368_V0.8951.00
341_A346_G0.8951.00
355_I384_A0.8951.00
256_L375_L0.8931.00
274_R277_K0.8911.00
134_V178_S0.8871.00
187_K190_E0.8851.00
3_I33_H0.8831.00
133_T174_V0.8801.00
124_L128_L0.8791.00
255_R258_D0.8761.00
256_L261_L0.8751.00
228_P231_K0.8751.00
262_I286_N0.8751.00
206_E209_R0.8721.00
247_E271_G0.8721.00
236_Y311_V0.8711.00
25_V29_A0.8701.00
329_L355_I0.8691.00
357_V380_H0.8681.00
112_V138_Q0.8671.00
329_L384_A0.8671.00
187_K191_K0.8651.00
274_R278_M0.8621.00
252_A368_V0.8591.00
178_S201_F0.8551.00
360_E363_S0.8511.00
329_L387_Y0.8511.00
19_K295_Y0.8491.00
399_R403_N0.8481.00
307_C374_A0.8471.00
20_Y138_Q0.8471.00
206_E210_F0.8461.00
302_L333_G0.8441.00
203_N319_A0.8411.00
336_V370_G0.8411.00
310_V345_L0.8391.00
241_G270_G0.8361.00
189_I198_V0.8301.00
277_K280_Q0.8291.00
93_S97_P0.8291.00
31_Q395_E0.8281.00
251_E255_R0.8271.00
235_L302_L0.8191.00
349_C358_C0.8191.00
20_Y203_N0.8181.00
23_E206_E0.8171.00
169_S173_N0.8151.00
252_A364_V0.8151.00
126_A133_T0.8151.00
254_D284_L0.8101.00
309_L371_I0.8091.00
310_V321_L0.8061.00
334_N374_A0.8051.00
183_S186_N0.8021.00
365_E369_A0.8021.00
251_E254_D0.8001.00
340_E345_L0.7991.00
36_R40_A0.7991.00
353_P373_Q0.7951.00
334_N381_N0.7941.00
132_R175_D0.7921.00
369_A372_R0.7891.00
307_C371_I0.7871.00
134_V176_N0.7841.00
242_E247_E0.7821.00
399_R402_I0.7821.00
393_D397_V0.7811.00
199_I400_Q0.7771.00
242_E271_G0.7771.00
295_Y299_P0.7741.00
236_Y308_H0.7741.00
23_E27_W0.7731.00
339_A358_C0.7721.00
395_E399_R0.7721.00
301_L304_M0.7701.00
300_A304_M0.7701.00
244_Q315_G0.7701.00
111_G117_F0.7671.00
394_K398_L0.7671.00
165_A168_R0.7641.00
279_A287_M0.7641.00
373_Q376_L0.7601.00
165_A169_S0.7601.00
201_F404_D0.7581.00
98_L104_W0.7581.00
244_Q312_Q0.7571.00
236_Y263_F0.7571.00
237_S308_H0.7561.00
256_L259_E0.7561.00
351_T354_G0.7521.00
387_Y392_L0.7521.00
300_A303_K0.7511.00
229_D232_K0.7471.00
183_S187_K0.7431.00
351_T355_I0.7391.00
7_G21_T0.7351.00
139_D243_K0.7341.00
238_G246_L0.7341.00
15_T239_N0.7341.00
365_E368_V0.7291.00
202_P400_Q0.7291.00
180_I319_A0.7241.00
138_Q180_I0.7221.00
202_P347_Q0.7211.00
318_D321_L0.7181.00
112_V136_H0.7181.00
199_I202_P0.7151.00
240_I272_K0.7131.00
199_I404_D0.7111.00
234_I375_L0.7111.00
108_R405_I0.7081.00
92_V96_F0.7071.00
20_Y401_F0.7061.00
110_I401_F0.7061.00
242_E313_K0.7051.00
108_R134_V0.7041.00
293_Q298_L0.7031.00
29_A35_V0.7031.00
269_G272_K0.7031.00
276_E289_F0.6951.00
202_P392_L0.6941.00
19_K22_G0.6941.00
234_I261_L0.6941.00
213_V303_K0.6891.00
401_F405_I0.6881.00
350_E353_P0.6861.00
346_G358_C0.6831.00
29_A64_A0.6811.00
5_V35_V0.6811.00
126_A129_S0.6811.00
122_M126_A0.6781.00
7_G17_I0.6741.00
31_Q398_L0.6741.00
309_L367_L0.6741.00
336_V374_A0.6691.00
329_L381_N0.6681.00
34_E66_T0.6681.00
338_T361_P0.6681.00
252_A371_I0.6641.00
161_Q164_T0.6641.00
383_V387_Y0.6611.00
338_T367_L0.6601.00
129_S132_R0.6591.00
384_A387_Y0.6551.00
26_E30_A0.6541.00
30_A395_E0.6541.00
326_T392_L0.6531.00
168_R172_H0.6521.00
245_G362_E0.6491.00
25_V35_V0.6481.00
279_A282_R0.6461.00
24_M401_F0.6461.00
26_E63_G0.6451.00
351_T357_V0.6451.00
253_A256_L0.6421.00
113_V116_L0.6401.00
207_I330_A0.6351.00
312_Q318_D0.6351.00
201_F400_Q0.6341.00
207_I389_E0.6331.00
93_S96_F0.6301.00
359_V366_A0.6291.00
382_T385_R0.6291.00
201_F401_F0.6281.00
238_G244_Q0.6261.00
301_L305_G0.6241.00
308_H335_A0.6231.00
27_W394_K0.6211.00
385_R388_A0.6211.00
245_G311_V0.6201.00
246_L271_G0.6151.00
127_K173_N0.6141.00
32_G402_I0.6141.00
5_V110_I0.6121.00
250_I287_M0.6121.00
63_G66_T0.6121.00
271_G275_L0.6111.00
182_R202_P0.6101.00
259_E376_L0.6091.00
29_A33_H0.6081.00
349_C355_I0.6051.00
9_N18_G0.6051.00
136_H401_F0.6041.00
307_C336_V0.6021.00
232_K235_L0.6011.00
179_T200_F0.6001.00
4_L36_R0.5961.00
398_L402_I0.5951.00
240_I246_L0.5911.00
183_S346_G0.5901.00
259_E372_R0.5901.00
109_I131_A0.5881.00
257_R261_L0.5881.00
161_Q165_A0.5861.00
239_N244_Q0.5861.00
208_A211_Q0.5861.00
209_R295_Y0.5861.00
184_M187_K0.5861.00
106_P131_A0.5851.00
355_I383_V0.5851.00
267_G291_P0.5841.00
90_F94_S0.5821.00
263_F287_M0.5801.00
17_I113_V0.5781.00
189_I192_G0.5771.00
311_V338_T0.5771.00
328_I335_A0.5771.00
125_L128_L0.5771.00
39_T67_V0.5741.00
20_Y112_V0.5731.00
14_L292_L0.5721.00
140_Y143_D0.5711.00
252_A256_L0.5701.00
211_Q214_A0.5691.00
5_V405_I0.5671.00
181_S184_M0.5671.00
138_Q203_N0.5671.00
317_A392_L0.5671.00
248_N252_A0.5661.00
109_I126_A0.5661.00
185_M195_A0.5661.00
266_V298_L0.5631.00
66_T69_R0.5621.00
336_V371_I0.5611.00
339_A361_P0.5611.00
302_L308_H0.5591.00
244_Q320_V0.5591.00
32_G35_V0.5581.00
103_R106_P0.5571.00
25_V65_A0.5571.00
206_E394_K0.5551.00
211_Q303_K0.5521.00
250_I275_L0.5511.00
169_S172_H0.5501.00
20_Y24_M0.5491.00
245_G338_T0.5491.00
207_I388_A0.5491.00
235_L308_H0.5471.00
326_T348_L0.5471.00
235_L264_A0.5461.00
275_L278_M0.5461.00
321_L348_L0.5451.00
275_L289_F0.5441.00
233_I301_L0.5431.00
321_L335_A0.5421.00
24_M27_W0.5421.00
134_V405_I0.5411.00
329_L388_A0.5401.00
26_E29_A0.5371.00
340_E346_G0.5371.00
310_V318_D0.5351.00
230_N260_P0.5331.00
257_R260_P0.5331.00
102_R129_S0.5331.00
249_V253_A0.5331.00
136_H178_S0.5321.00
341_A344_E0.5321.00
183_S347_Q0.5291.00
133_T175_D0.5291.00
279_A285_R0.5281.00
321_L337_I0.5281.00
247_E275_L0.5271.00
94_S97_P0.5271.00
184_M316_A0.5261.00
260_P286_N0.5261.00
235_L333_G0.5231.00
69_R72_L0.5221.00
232_K378_P0.5201.00
228_P262_I0.5171.00
108_R132_R0.5161.00
307_C375_L0.5151.00
268_Q292_L0.5141.00
4_L109_I0.5141.00
105_K131_A0.5131.00
106_P109_I0.5121.00
242_E274_R0.5101.00
123_R133_T0.5101.00
202_P397_V0.5101.00
284_L287_M0.5091.00
176_N180_I0.5081.00
364_V367_L0.5081.00
176_N197_N0.5081.00
332_G381_N0.5081.00
111_G122_M0.5081.00
162_L165_A0.5071.00
202_P391_T0.5071.00
210_F330_A0.5041.00
137_I140_Y0.5031.00
252_A309_L0.5031.00
276_E279_A0.5031.00
375_L378_P0.5011.00
205_S330_A0.5011.00
99_M106_P0.5001.00
23_E319_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1rzuA 1 0.9705 100 0.189 Contact Map
3c48A 2 0.914 100 0.189 Contact Map
2r60A 1 0.9631 100 0.192 Contact Map
3froA 3 0.9607 100 0.2 Contact Map
2qzsA 1 0.973 100 0.209 Contact Map
4n9wA 1 0.8354 100 0.211 Contact Map
3s28A 4 0.9656 100 0.226 Contact Map
3okpA 1 0.8821 100 0.228 Contact Map
2jjmA 4 0.8575 100 0.229 Contact Map
3vueA 1 0.9631 100 0.244 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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