GREMLIN Database
THIB - Thiamine-binding periplasmic protein
UniProt: P31550 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11574
Length: 327 (290)
Sequences: 35636 (27905)
Seq/√Len: 1638.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
21_V53_K3.5401.00
131_K232_A3.1761.00
23_T53_K3.0491.00
97_A256_P3.0441.00
132_N230_N2.6351.00
21_V51_E2.6261.00
10_L13_T2.5351.00
50_C258_L2.5241.00
48_C261_K2.4821.00
184_Q188_K2.4811.00
8_L11_L2.4221.00
43_A265_F2.3851.00
145_V185_K2.3131.00
181_Q185_K2.3051.00
45_E51_E2.2881.00
268_S271_F2.2631.00
185_K189_K2.2531.00
38_P42_K2.2461.00
9_L12_C2.1461.00
43_A46_A2.1161.00
261_K264_Q2.1141.00
140_S143_E2.0481.00
43_A274_A2.0091.00
7_P10_L1.9431.00
62_S65_N1.9411.00
273_N277_T1.9211.00
134_L211_L1.8951.00
77_D255_Q1.8911.00
20_P258_L1.8851.00
23_T55_V1.8771.00
44_F261_K1.8771.00
6_L9_L1.8731.00
129_Y232_A1.8681.00
178_D181_Q1.8661.00
255_Q258_L1.8511.00
133_K210_D1.8481.00
39_V42_K1.8291.00
96_F248_A1.8281.00
25_Y70_E1.8171.00
43_A47_D1.7821.00
42_K46_A1.7791.00
11_L14_A1.7561.00
44_F48_C1.7511.00
41_K262_F1.7491.00
45_E48_C1.7441.00
147_S189_K1.7391.00
41_K45_E1.7281.00
79_V250_T1.7051.00
147_S151_W1.7021.00
102_A260_Q1.6931.00
131_K135_K1.6881.00
143_E146_E1.6791.00
257_E261_K1.6771.00
107_N286_N1.6561.00
182_A185_K1.6521.00
58_E62_S1.6491.00
117_F246_V1.6491.00
196_G199_E1.6361.00
190_T193_V1.6081.00
5_C8_L1.5861.00
130_D133_K1.5861.00
269_P273_N1.5851.00
132_N136_N1.5651.00
66_R70_E1.5461.00
203_L208_E1.5401.00
256_P260_Q1.5401.00
291_A295_K1.5301.00
97_A251_A1.5291.00
47_D268_S1.4981.00
26_T54_L1.4941.00
271_F274_A1.4911.00
42_K45_E1.4851.00
181_Q184_Q1.4621.00
44_F262_F1.4471.00
274_A277_T1.4401.00
103_A106_V1.4391.00
20_P255_Q1.4381.00
44_F265_F1.4321.00
139_Q143_E1.4311.00
258_L261_K1.4251.00
91_S116_T1.4121.00
39_V43_A1.4081.00
44_F50_C1.4071.00
249_R263_L1.4041.00
142_K145_V1.4041.00
131_K230_N1.3671.00
127_F234_A1.3551.00
138_P143_E1.3531.00
182_A186_L1.3511.00
202_G206_K1.3481.00
129_Y234_A1.3321.00
110_G113_N1.3231.00
274_A278_G1.3131.00
154_I209_S1.3121.00
253_S256_P1.3081.00
185_K188_K1.2961.00
105_A113_N1.2951.00
142_K146_E1.2921.00
41_K54_L1.2831.00
78_V259_A1.2811.00
104_D107_N1.2721.00
145_V189_K1.2661.00
291_A294_E1.2601.00
41_K52_L1.2591.00
126_A233_A1.2501.00
78_V262_F1.2401.00
148_D152_R1.2361.00
145_V186_L1.2331.00
203_L209_S1.2331.00
269_P286_N1.2131.00
190_T194_T1.2101.00
171_M176_G1.2091.00
171_M174_V1.2041.00
260_Q264_Q1.2031.00
257_E260_Q1.2011.00
44_F258_L1.2011.00
145_V182_A1.1741.00
172_Q175_Y1.1741.00
249_R259_A1.1671.00
50_C261_K1.1581.00
247_A263_L1.1431.00
171_M175_Y1.1331.00
22_L258_L1.1291.00
43_A270_A1.1231.00
9_L13_T1.1211.00
170_W175_Y1.1201.00
249_R260_Q1.1101.00
39_V274_A1.0951.00
130_D229_D1.0901.00
94_G251_A1.0881.00
98_K101_V1.0811.00
269_P285_A1.0811.00
262_F266_M1.0801.00
22_L262_F1.0671.00
77_D253_S1.0661.00
47_D270_A1.0661.00
127_F211_L1.0641.00
68_R93_T1.0591.00
57_L66_R1.0591.00
152_R210_D1.0521.00
270_A273_N1.0451.00
147_S150_N1.0421.00
108_V113_N1.0411.00
183_W187_A1.0371.00
108_V112_W1.0301.00
133_K136_N1.0221.00
199_E202_G1.0211.00
133_K149_Q1.0201.00
78_V263_L1.0071.00
82_L248_A1.0041.00
191_V194_T1.0021.00
265_F271_F1.0021.00
20_P49_N1.0001.00
79_V248_A0.9891.00
250_T253_S0.9891.00
270_A274_A0.9841.00
225_E228_K0.9831.00
130_D230_N0.9811.00
64_L86_L0.9771.00
67_L79_V0.9771.00
149_Q152_R0.9751.00
24_V247_A0.9701.00
251_A256_P0.9661.00
120_F247_A0.9651.00
107_N285_A0.9651.00
40_V271_F0.9651.00
88_D92_K0.9611.00
172_Q177_D0.9611.00
128_V214_S0.9601.00
75_K250_T0.9601.00
8_L13_T0.9551.00
178_D182_A0.9521.00
132_N229_D0.9521.00
109_P112_W0.9501.00
191_V195_K0.9481.00
249_R253_S0.9451.00
105_A108_V0.9451.00
39_V278_G0.9421.00
47_D265_F0.9401.00
273_N287_V0.9381.00
117_F248_A0.9371.00
296_L299_P0.9341.00
97_A253_S0.9331.00
204_F231_Y0.9321.00
153_V156_Q0.9311.00
7_P11_L0.9281.00
253_S259_A0.9281.00
43_A271_F0.9271.00
290_P294_E0.9261.00
149_Q210_D0.9251.00
129_Y137_P0.9211.00
109_P113_N0.9141.00
13_T17_F0.9121.00
25_Y76_A0.9111.00
8_L12_C0.9101.00
247_A266_M0.9071.00
97_A260_Q0.9031.00
96_F116_T0.8981.00
262_F265_F0.8951.00
89_A93_T0.8941.00
52_L262_F0.8921.00
13_T16_V0.8901.00
292_G295_K0.8881.00
132_N135_K0.8851.00
14_A18_A0.8841.00
45_E49_N0.8771.00
10_L14_A0.8751.00
98_K116_T0.8681.00
134_L149_Q0.8631.00
297_T300_A0.8611.00
200_A212_V0.8601.00
66_R69_M0.8591.00
90_A96_F0.8541.00
45_E52_L0.8531.00
104_D264_Q0.8441.00
19_K51_E0.8401.00
40_V43_A0.8371.00
91_S96_F0.8331.00
167_L171_M0.8281.00
14_A17_F0.8201.00
20_P50_C0.8191.00
198_S201_Y0.8121.00
140_S238_E0.8101.00
49_N261_K0.8031.00
106_V109_P0.8001.00
144_L211_L0.7991.00
65_N68_R0.7981.00
25_Y57_L0.7951.00
97_A249_R0.7851.00
65_N69_M0.7831.00
97_A250_T0.7821.00
264_Q268_S0.7811.00
15_P18_A0.7781.00
295_K298_K0.7781.00
259_A263_L0.7771.00
123_G243_Q0.7771.00
166_G169_L0.7761.00
64_L68_R0.7761.00
103_A264_Q0.7711.00
154_I157_D0.7691.00
16_V19_K0.7691.00
55_V66_R0.7681.00
141_L145_V0.7671.00
170_W174_V0.7601.00
184_Q187_A0.7591.00
202_G205_L0.7591.00
99_S263_L0.7591.00
36_P39_V0.7581.00
148_D151_W0.7561.00
106_V110_G0.7501.00
149_Q211_L0.7481.00
229_D232_A0.7461.00
97_A101_V0.7451.00
9_L14_A0.7431.00
64_L90_A0.7391.00
139_Q237_S0.7301.00
100_G260_Q0.7291.00
232_A235_N0.7261.00
106_V113_N0.7241.00
70_E74_S0.7201.00
80_L247_A0.7181.00
169_L172_Q0.7141.00
79_V96_F0.7091.00
22_L255_Q0.7051.00
220_A223_I0.7041.00
137_P143_E0.7031.00
180_P184_Q0.7001.00
192_T195_K0.6991.00
48_C258_L0.6951.00
45_E50_C0.6901.00
173_K176_G0.6861.00
169_L173_K0.6841.00
5_C9_L0.6831.00
129_Y134_L0.6821.00
87_L117_F0.6781.00
64_L89_A0.6771.00
221_Y224_L0.6761.00
40_V265_F0.6761.00
168_L171_M0.6751.00
153_V157_D0.6741.00
6_L11_L0.6741.00
83_D86_L0.6721.00
128_V233_A0.6721.00
97_A252_A0.6721.00
180_P183_W0.6641.00
296_L300_A0.6641.00
103_A107_N0.6631.00
108_V267_V0.6611.00
82_L90_A0.6591.00
12_C15_P0.6571.00
20_P261_K0.6561.00
137_P234_A0.6541.00
179_A183_W0.6531.00
154_I159_R0.6521.00
272_Q284_V0.6521.00
40_V266_M0.6461.00
217_T281_M0.6451.00
203_L206_K0.6441.00
102_A257_E0.6431.00
170_W173_K0.6421.00
99_S249_R0.6421.00
12_C16_V0.6421.00
201_Y205_L0.6411.00
134_L210_D0.6381.00
166_G170_W0.6341.00
229_D235_N0.6331.00
256_P259_A0.6311.00
168_L172_Q0.6301.00
12_C18_A0.6301.00
73_N252_A0.6291.00
11_L15_P0.6241.00
132_N207_G0.6241.00
174_V177_D0.6241.00
13_T19_K0.6231.00
99_S260_Q0.6191.00
219_P223_I0.6171.00
198_S202_G0.6161.00
11_L17_F0.6151.00
91_S94_G0.6131.00
220_A224_L0.6131.00
25_Y55_V0.6121.00
86_L89_A0.6111.00
154_I158_P0.6101.00
7_P12_C0.6091.00
12_C19_K0.6081.00
23_T77_D0.6071.00
28_D32_A0.6061.00
135_K230_N0.6051.00
77_D254_K0.6031.00
166_G171_M0.6021.00
127_F213_L0.6011.00
62_S66_R0.6011.00
249_R256_P0.6001.00
141_L182_A0.6001.00
10_L16_V0.5991.00
124_Y216_T0.5971.00
123_G245_E0.5961.00
15_P19_K0.5951.00
51_E54_L0.5951.00
80_L120_F0.5931.00
128_V231_Y0.5921.00
101_V260_Q0.5921.00
153_V158_P0.5911.00
138_P211_L0.5911.00
24_V54_L0.5901.00
165_L171_M0.5891.00
12_C17_F0.5871.00
131_K229_D0.5861.00
107_N110_G0.5841.00
60_G86_L0.5841.00
36_P40_V0.5841.00
23_T26_T0.5831.00
261_K265_F0.5811.00
79_V82_L0.5811.00
121_D245_E0.5801.00
107_N269_P0.5791.00
155_Y158_P0.5781.00
24_V41_K0.5771.00
289_L294_E0.5751.00
173_K177_D0.5741.00
255_Q259_A0.5731.00
190_T195_K0.5731.00
24_V78_V0.5731.00
11_L18_A0.5711.00
69_M72_K0.5711.00
289_L292_G0.5711.00
130_D207_G0.5701.00
165_L168_L0.5681.00
55_V76_A0.5681.00
11_L16_V0.5671.00
260_Q263_L0.5671.00
78_V247_A0.5671.00
201_Y214_S0.5641.00
214_S219_P0.5631.00
118_V263_L0.5631.00
26_T31_A0.5631.00
105_A109_P0.5631.00
219_P222_H0.5631.00
77_D259_A0.5601.00
169_L175_Y0.5601.00
97_A102_A0.5601.00
222_H226_E0.5591.00
168_L175_Y0.5591.00
118_V267_V0.5571.00
125_F215_Y0.5561.00
99_S118_V0.5561.00
87_L91_S0.5551.00
41_K51_E0.5541.00
252_A256_P0.5541.00
20_P23_T0.5521.00
172_Q176_G0.5521.00
90_A95_L0.5521.00
9_L15_P0.5511.00
20_P48_C0.5511.00
87_L248_A0.5471.00
24_V262_F0.5451.00
104_D109_P0.5441.00
60_G64_L0.5431.00
112_W122_Y0.5431.00
13_T18_A0.5431.00
40_V274_A0.5411.00
199_E203_L0.5401.00
269_P272_Q0.5391.00
204_F212_V0.5371.00
139_Q144_L0.5371.00
22_L259_A0.5361.00
197_W200_A0.5361.00
128_V220_A0.5361.00
22_L77_D0.5351.00
45_E262_F0.5351.00
75_K252_A0.5341.00
277_T292_G0.5321.00
174_V178_D0.5271.00
26_T37_G0.5261.00
272_Q276_P0.5261.00
200_A209_S0.5261.00
68_R89_A0.5251.00
22_L52_L0.5241.00
171_M177_D0.5231.00
272_Q285_A0.5231.00
169_L174_V0.5201.00
158_P161_S0.5161.00
77_D256_P0.5141.00
122_Y284_V0.5141.00
6_L10_L0.5131.00
267_V272_Q0.5131.00
102_A256_P0.5131.00
91_S114_N0.5111.00
108_V111_G0.5091.00
48_C265_F0.5081.00
159_R163_P0.5051.00
63_L79_V0.5041.00
88_D91_S0.5021.00
167_L172_Q0.5021.00
164_G171_M0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qryA 1 0.9725 100 0.233 Contact Map
3pu5A 1 0.9297 100 0.285 Contact Map
3rpwA 1 0.9113 100 0.291 Contact Map
4edpA 1 0.9083 100 0.303 Contact Map
3uorA 1 0.9205 100 0.307 Contact Map
3qufA 1 0.9358 100 0.315 Contact Map
4r6kA 1 0.9358 100 0.317 Contact Map
2pt1A 1 0.9205 100 0.32 Contact Map
3c9hA 1 0.9511 100 0.32 Contact Map
3waeA 2 0.8899 100 0.321 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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