GREMLIN Database
POTF - Putrescine-binding periplasmic protein
UniProt: P31133 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11629
Length: 370 (323)
Sequences: 36515 (29200)
Seq/√Len: 1624.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
31_T58_V3.7681.00
144_V265_S3.6441.00
33_H58_V3.2671.00
99_Q289_K3.2481.00
164_L216_K2.9461.00
31_T56_K2.9251.00
145_D263_N2.8211.00
100_P127_N2.8021.00
159_S162_L2.4641.00
67_V71_K2.4561.00
55_I291_E2.4451.00
53_T294_Q2.4271.00
50_E56_K2.4191.00
48_N298_Y2.3971.00
301_R304_V2.3311.00
216_K220_N2.3121.00
215_L219_P2.2791.00
48_N51_K2.2571.00
212_D216_K2.2371.00
142_Y265_S2.2301.00
204_D209_P2.2201.00
168_N220_N2.2021.00
294_Q297_N2.1871.00
33_H60_D2.1141.00
306_A310_D2.0531.00
144_V148_K2.0441.00
102_D127_N2.0311.00
48_N307_H2.0231.00
80_D288_N2.0201.00
47_A51_K2.0001.00
153_E166_P1.9921.00
30_K291_E1.9711.00
48_N52_E1.9671.00
288_N291_E1.9531.00
146_K239_C1.9361.00
209_P212_D1.9321.00
98_F281_A1.9211.00
44_D47_A1.9021.00
130_A279_V1.8821.00
165_K168_N1.8801.00
213_L216_K1.8601.00
111_N320_A1.8241.00
207_T212_D1.7951.00
49_F294_Q1.7891.00
162_L165_K1.7871.00
104_S293_Y1.7691.00
147_V240_V1.7551.00
46_V295_F1.7481.00
123_H128_K1.7441.00
290_D294_Q1.7261.00
46_V50_E1.7231.00
302_P306_A1.7171.00
82_V283_P1.7141.00
29_Q56_K1.7131.00
304_V307_H1.7001.00
99_Q284_A1.6991.00
49_F53_T1.6971.00
168_N172_L1.6961.00
165_K169_L1.6561.00
50_E53_T1.6411.00
307_H311_H1.6331.00
232_D237_D1.6251.00
161_D164_L1.6011.00
289_K293_Y1.6001.00
291_E294_Q1.5991.00
164_L217_L1.5981.00
155_A158_D1.5861.00
52_E301_R1.5831.00
164_L168_N1.5821.00
145_D149_A1.5751.00
114_P118_K1.5491.00
44_D48_N1.5471.00
164_L220_N1.5431.00
178_S238_I1.5351.00
142_Y267_S1.5311.00
36_N59_Y1.5221.00
143_N146_K1.5211.00
144_V263_N1.5171.00
47_A50_E1.5111.00
169_L173_K1.5031.00
30_K288_N1.4951.00
231_N235_N1.4741.00
307_H310_D1.4641.00
213_L217_L1.4561.00
212_D215_L1.4541.00
205_D209_P1.4451.00
286_A289_K1.4451.00
118_K123_H1.4401.00
164_L213_L1.4261.00
81_L292_A1.4101.00
49_F295_F1.4041.00
327_A331_E1.3821.00
282_M296_L1.3591.00
67_V70_G1.3541.00
140_I267_S1.3531.00
24_T27_A1.3491.00
170_E239_C1.3411.00
161_D165_K1.3271.00
139_G266_F1.3241.00
17_L20_V1.3241.00
166_P169_L1.3131.00
336_Y340_D1.3091.00
221_I224_F1.3051.00
225_H228_Q1.3041.00
49_F298_Y1.3041.00
108_E297_N1.3031.00
93_L129_F1.2901.00
158_D162_L1.2751.00
327_A330_R1.2641.00
204_D207_T1.2451.00
35_Y71_K1.2411.00
100_P103_K1.2341.00
232_D238_I1.2291.00
293_Y297_N1.2291.00
320_A323_P1.2271.00
122_K279_V1.2241.00
146_K170_E1.2201.00
46_V59_Y1.2151.00
49_F55_I1.2131.00
233_L264_V1.2081.00
290_D293_Y1.2081.00
286_A292_A1.2031.00
282_M292_A1.1861.00
111_N318_N1.1851.00
228_Q231_N1.1841.00
153_E169_L1.1651.00
302_P320_A1.1631.00
55_I294_Q1.1471.00
151_L170_E1.1451.00
303_D306_A1.1381.00
259_K262_V1.1351.00
81_L295_F1.1351.00
141_G243_G1.1341.00
140_I240_V1.1331.00
100_P128_K1.1311.00
216_K219_P1.1231.00
21_S24_T1.1201.00
84_P281_A1.1131.00
14_A18_M1.1131.00
16_A19_A1.1091.00
113_D319_K1.0981.00
318_N321_A1.0891.00
32_L295_F1.0871.00
80_D286_A1.0861.00
143_N262_V1.0781.00
209_P213_L1.0711.00
48_N303_D1.0701.00
114_P117_L1.0681.00
52_E298_Y1.0641.00
49_F291_E1.0591.00
298_Y304_V1.0571.00
44_D307_H1.0541.00
34_I280_F1.0531.00
167_E240_V1.0521.00
197_D200_S1.0491.00
147_V166_P1.0491.00
151_L173_K1.0481.00
143_N263_N1.0391.00
50_E54_G1.0381.00
96_G284_A1.0311.00
145_D262_V1.0291.00
250_Q253_N1.0271.00
107_P110_K1.0251.00
166_P240_V1.0241.00
158_D270_K1.0231.00
303_D307_H1.0211.00
92_Q98_F1.0201.00
46_V57_V1.0131.00
169_L172_L1.0111.00
146_K151_L1.0101.00
17_L21_S1.0081.00
82_V281_A1.0071.00
295_F299_L1.0071.00
90_E94_T0.9941.00
282_M286_A0.9891.00
147_V153_E0.9891.00
197_D201_T0.9881.00
284_A289_K0.9841.00
283_P286_A0.9801.00
302_P318_N0.9791.00
52_E303_D0.9771.00
282_M293_Y0.9751.00
243_G247_D0.9731.00
249_W253_N0.9731.00
338_P341_V0.9661.00
93_L124_D0.9571.00
45_T48_N0.9561.00
81_L296_L0.9531.00
280_F296_L0.9521.00
78_G283_P0.9521.00
32_L291_E0.9511.00
146_K149_A0.9481.00
16_A20_V0.9441.00
45_T304_V0.9431.00
70_G74_A0.9411.00
163_I240_V0.9401.00
170_E174_S0.9371.00
133_Y280_F0.9361.00
91_R95_A0.9331.00
106_L109_W0.9331.00
44_D311_H0.9281.00
163_I167_E0.9191.00
215_L218_R0.9151.00
15_G19_A0.9111.00
105_K108_E0.9111.00
13_V17_L0.9081.00
148_K156_P0.9081.00
48_N304_V0.9051.00
194_L209_P0.9051.00
188_A192_N0.9031.00
99_Q293_Y0.9011.00
7_K10_S0.8981.00
292_A296_L0.8951.00
295_F298_Y0.8941.00
203_A207_T0.8931.00
306_A321_A0.8891.00
145_D148_K0.8811.00
157_V162_L0.8781.00
13_V16_A0.8751.00
35_Y79_F0.8711.00
112_L300_L0.8651.00
130_A281_A0.8621.00
328_E331_E0.8601.00
176_G239_C0.8601.00
202_K205_D0.8601.00
106_L297_N0.8591.00
115_E118_K0.8561.00
123_H126_D0.8541.00
203_A206_Y0.8521.00
221_I225_H0.8491.00
171_K174_S0.8471.00
160_W164_L0.8371.00
35_Y62_F0.8351.00
142_Y156_P0.8311.00
9_L12_L0.8261.00
336_Y339_A0.8191.00
18_M21_S0.8191.00
122_K128_K0.8171.00
231_N234_A0.8061.00
190_V194_L0.8021.00
75_G285_D0.7981.00
38_S42_A0.7941.00
187_F191_L0.7881.00
260_N264_V0.7881.00
155_A162_L0.7771.00
111_N302_P0.7751.00
39_D43_P0.7731.00
166_P170_E0.7721.00
66_E70_G0.7601.00
151_L169_L0.7601.00
156_P162_L0.7581.00
297_N301_R0.7551.00
12_L15_G0.7541.00
229_Y241_A0.7531.00
12_L16_A0.7531.00
57_V295_F0.7521.00
326_S330_R0.7501.00
331_E334_G0.7471.00
117_L128_K0.7461.00
100_P129_F0.7451.00
189_T192_N0.7441.00
50_E57_V0.7431.00
30_K55_I0.7411.00
98_F129_F0.7411.00
14_A17_L0.7381.00
337_P341_V0.7371.00
112_L117_L0.7371.00
339_A342_R0.7371.00
105_K293_Y0.7371.00
280_F299_L0.7361.00
185_E189_T0.7361.00
38_S41_I0.7351.00
153_E170_E0.7341.00
319_K323_P0.7341.00
167_E171_K0.7331.00
93_L98_F0.7331.00
20_V24_T0.7231.00
72_L97_V0.7221.00
211_T215_L0.7211.00
28_E31_T0.7191.00
332_N335_I0.7151.00
99_Q103_K0.7151.00
99_Q283_P0.7151.00
141_G248_V0.7151.00
99_Q282_M0.7131.00
186_V190_V0.7121.00
69_E73_M0.7111.00
14_A20_V0.7111.00
40_Y43_P0.7091.00
83_V280_F0.7071.00
85_S88_F0.7071.00
227_S230_I0.7071.00
20_V23_G0.7061.00
175_C178_S0.7061.00
72_L78_G0.7051.00
113_D116_L0.7031.00
69_E91_R0.7011.00
101_L296_L0.7011.00
39_D42_A0.6991.00
337_P340_D0.6981.00
148_K263_N0.6971.00
164_L167_E0.6971.00
99_Q286_A0.6961.00
29_Q54_G0.6921.00
171_K176_G0.6911.00
32_L288_N0.6861.00
230_I234_A0.6831.00
196_K199_N0.6791.00
141_G266_F0.6791.00
102_D128_K0.6781.00
104_S290_D0.6761.00
50_E55_I0.6751.00
60_D79_F0.6741.00
141_G264_V0.6721.00
45_T307_H0.6721.00
10_S14_A0.6691.00
151_L174_S0.6681.00
114_P319_K0.6681.00
71_K77_T0.6671.00
202_K206_Y0.6661.00
330_R334_G0.6651.00
111_N319_K0.6651.00
56_K59_Y0.6641.00
68_L71_K0.6611.00
196_K205_D0.6601.00
33_H80_D0.6601.00
89_L130_A0.6601.00
136_A276_F0.6581.00
192_N197_D0.6571.00
156_P267_S0.6571.00
8_W11_G0.6571.00
34_I81_L0.6561.00
71_K74_A0.6551.00
338_P342_R0.6541.00
171_K175_C0.6511.00
191_L196_K0.6501.00
294_Q298_Y0.6501.00
198_P201_T0.6501.00
18_M22_V0.6481.00
328_E332_N0.6471.00
66_E69_E0.6461.00
249_W252_S0.6411.00
191_L206_Y0.6411.00
227_S231_N0.6391.00
248_V252_S0.6341.00
228_Q232_D0.6341.00
143_N236_G0.6331.00
15_G18_M0.6311.00
45_T298_Y0.6301.00
222_R226_S0.6301.00
82_V98_F0.6291.00
196_K206_Y0.6291.00
10_S13_V0.6281.00
11_G15_G0.6281.00
102_D293_Y0.6261.00
22_V25_L0.6251.00
145_D236_G0.6241.00
289_K292_A0.6181.00
70_G73_M0.6171.00
163_I177_V0.6161.00
181_D184_E0.6141.00
34_I59_Y0.6131.00
24_T28_E0.6131.00
80_D292_A0.6131.00
32_L292_A0.6111.00
194_L213_L0.6101.00
7_K19_A0.6081.00
142_Y147_V0.6071.00
222_R225_H0.6071.00
160_W213_L0.6071.00
210_A214_L0.6061.00
232_D235_N0.6051.00
30_K33_H0.6051.00
35_Y60_D0.6031.00
69_E72_L0.6011.00
89_L93_L0.6011.00
156_P166_P0.6011.00
103_K127_N0.6011.00
330_R333_P0.5991.00
313_F316_N0.5981.00
23_G26_A0.5981.00
319_K322_T0.5971.00
148_K151_L0.5961.00
112_L116_L0.5961.00
13_V20_V0.5951.00
333_P336_Y0.5941.00
147_V170_E0.5941.00
113_D322_T0.5921.00
142_Y240_V0.5921.00
80_D287_K0.5911.00
93_L96_G0.5901.00
88_F91_R0.5891.00
326_S329_V0.5891.00
53_T291_E0.5881.00
230_I243_G0.5871.00
9_L20_V0.5861.00
148_K152_G0.5861.00
202_K207_T0.5861.00
168_N171_K0.5851.00
162_L166_P0.5851.00
223_Y226_S0.5831.00
99_Q285_D0.5811.00
279_V283_P0.5801.00
186_V194_L0.5781.00
99_Q104_S0.5781.00
260_N263_N0.5781.00
151_L166_P0.5761.00
187_F190_V0.5741.00
34_I83_V0.5731.00
68_L82_V0.5721.00
159_S271_E0.5721.00
302_P305_V0.5721.00
60_D71_K0.5701.00
118_K122_K0.5681.00
10_S15_G0.5641.00
28_E294_Q0.5631.00
149_A174_S0.5621.00
81_L280_F0.5611.00
282_M289_K0.5611.00
13_V19_A0.5581.00
313_F317_A0.5571.00
333_P337_P0.5551.00
8_W12_L0.5541.00
10_S17_L0.5541.00
285_D289_K0.5511.00
140_I242_I0.5501.00
110_K113_D0.5501.00
176_G180_L0.5491.00
92_Q97_V0.5491.00
186_V189_T0.5461.00
9_L16_A0.5451.00
332_N336_Y0.5451.00
33_H36_N0.5441.00
108_E111_N0.5431.00
185_E188_A0.5431.00
185_E191_L0.5421.00
104_S289_K0.5411.00
247_D251_A0.5411.00
137_T245_A0.5401.00
111_N114_P0.5391.00
333_P338_P0.5391.00
10_S20_V0.5391.00
19_A22_V0.5391.00
45_T299_L0.5371.00
123_H127_N0.5371.00
191_L194_L0.5371.00
117_L123_H0.5361.00
116_L135_W0.5361.00
71_K75_G0.5351.00
316_N322_T0.5341.00
113_D117_L0.5341.00
170_E173_K0.5331.00
247_D250_Q0.5321.00
9_L13_V0.5311.00
246_G249_W0.5311.00
206_Y210_A0.5311.00
84_P92_Q0.5301.00
337_P342_R0.5281.00
97_V100_P0.5271.00
101_L282_M0.5271.00
106_L293_Y0.5251.00
128_K131_M0.5241.00
72_L283_P0.5241.00
140_I163_I0.5221.00
62_F67_V0.5221.00
168_N216_K0.5211.00
68_L72_L0.5211.00
196_K200_S0.5191.00
38_S43_P0.5181.00
205_D210_A0.5161.00
53_T295_F0.5151.00
30_K294_Q0.5151.00
105_K290_D0.5131.00
22_V26_A0.5131.00
12_L18_M0.5121.00
122_K130_A0.5121.00
305_V317_A0.5091.00
293_Y296_L0.5071.00
279_V282_M0.5071.00
239_C242_I0.5011.00
23_G27_A0.5011.00
14_A19_A0.5001.00
229_Y238_I0.5001.00
83_V133_Y0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3uorA 1 0.9027 100 0.204 Contact Map
4r2fA 1 0.8811 100 0.23 Contact Map
4mfiA 1 0.8919 100 0.239 Contact Map
4r9fA 1 0.8946 100 0.252 Contact Map
3ttmA 1 0.9216 100 0.266 Contact Map
3vxbA 1 0.8919 100 0.268 Contact Map
3ttnA 1 0.9027 100 0.282 Contact Map
4jdfA 1 0.9189 100 0.283 Contact Map
4g68A 1 0.8892 100 0.29 Contact Map
4r6kA 1 0.8973 100 0.301 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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