GREMLIN Database
EAMA - Probable amino-acid metabolite efflux pump
UniProt: P31125 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11639
Length: 299 (265)
Sequences: 38287 (27927)
Seq/√Len: 1715.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
79_A148_T3.2761.00
88_L145_F2.8761.00
8_L234_T2.8731.00
234_T238_R2.7651.00
259_A265_E2.6881.00
78_C147_L2.5751.00
44_A183_L2.4691.00
37_G190_F2.3901.00
75_F150_A2.3611.00
22_V219_Y2.3431.00
119_G281_G2.3111.00
234_T237_G2.2931.00
99_F152_A2.2101.00
110_E163_K2.1601.00
75_F147_L2.1191.00
24_K265_E2.0971.00
90_S255_G2.0941.00
211_M215_L2.0481.00
23_I77_F2.0331.00
103_L160_I2.0301.00
42_L221_A1.9981.00
9_A235_L1.9851.00
17_G254_V1.9701.00
41_M186_I1.9191.00
105_A112_L1.8861.00
101_I120_I1.8831.00
71_A154_S1.8761.00
95_A99_F1.7941.00
84_M145_F1.7861.00
100_T245_A1.7801.00
104_G110_E1.7631.00
48_I179_I1.7571.00
84_M144_G1.7531.00
146_M150_A1.7391.00
113_H116_Q1.7131.00
283_Y287_F1.7121.00
210_D213_T1.6751.00
123_A249_L1.6671.00
17_G258_S1.6561.00
173_A176_S1.6541.00
240_E243_R1.6481.00
187_I191_V1.6381.00
191_V194_L1.6031.00
14_V254_V1.5931.00
18_L219_Y1.5891.00
99_F156_A1.5841.00
75_F154_S1.5821.00
221_A226_I1.5711.00
57_P171_R1.5591.00
44_A186_I1.5571.00
268_T271_Q1.5481.00
142_M145_F1.5381.00
119_G285_N1.5131.00
162_N233_G1.4761.00
89_A148_T1.4641.00
30_M213_T1.4611.00
56_V60_L1.4561.00
26_G35_L1.4221.00
37_G186_I1.4191.00
99_F153_F1.4111.00
12_V235_L1.3981.00
104_G108_F1.3861.00
269_G273_L1.3781.00
78_C81_N1.3541.00
258_S262_L1.3541.00
114_G118_A1.3311.00
103_L107_T1.3231.00
126_G274_G1.3221.00
8_L231_I1.3221.00
43_V182_A1.3211.00
29_N213_T1.3181.00
119_G284_I1.3061.00
16_W228_G1.3041.00
129_V277_L1.3001.00
59_N63_G1.2981.00
46_P226_I1.2961.00
212_T215_L1.2881.00
84_M88_L1.2871.00
45_F49_F1.2801.00
57_P60_L1.2791.00
123_A281_G1.2761.00
86_A259_A1.2731.00
142_M146_M1.2691.00
272_F276_V1.2641.00
31_P34_M1.2601.00
25_V216_S1.2571.00
87_G256_L1.2531.00
156_A160_I1.2481.00
254_V258_S1.2471.00
211_M214_I1.2431.00
188_P191_V1.2391.00
250_L254_V1.2351.00
60_L161_F1.2331.00
21_V258_S1.2321.00
56_V59_N1.2211.00
276_V280_T1.2181.00
129_V270_L1.2181.00
85_P88_L1.2181.00
35_L220_L1.2131.00
91_L275_A1.2091.00
115_K284_I1.2021.00
24_K28_H1.2001.00
117_L121_A1.1821.00
212_T216_S1.1711.00
267_L272_F1.1541.00
215_L219_Y1.1521.00
206_L209_I1.1491.00
143_L147_L1.1481.00
103_L157_C1.1431.00
163_K240_E1.1411.00
63_G161_F1.1281.00
33_L192_A1.1271.00
7_V11_L1.1251.00
80_I89_A1.1231.00
57_P172_P1.1181.00
121_A125_F1.1161.00
44_A179_I1.1111.00
61_L65_G1.1031.00
130_L274_G1.0971.00
100_T241_T1.0921.00
37_G41_M1.0891.00
62_L184_I1.0831.00
12_V231_I1.0831.00
84_M148_T1.0761.00
126_G278_I1.0741.00
143_L146_M1.0721.00
62_L65_G1.0711.00
18_L258_S1.0681.00
37_G193_S1.0681.00
70_F188_P1.0641.00
60_L64_Y1.0591.00
110_E116_Q1.0581.00
70_F189_F1.0571.00
163_K241_T1.0561.00
12_V16_W1.0511.00
15_V223_V1.0511.00
105_A111_R1.0481.00
164_K167_S1.0461.00
114_G117_L1.0401.00
115_K288_G1.0371.00
59_N62_L1.0371.00
236_L244_V1.0351.00
163_K167_S1.0301.00
22_V216_S1.0301.00
101_I112_L1.0291.00
103_L156_A1.0231.00
63_G66_L1.0211.00
76_L80_I1.0201.00
66_L188_P1.0061.00
152_A156_A1.0051.00
26_G213_T1.0051.00
36_A77_F1.0041.00
16_W232_W1.0021.00
11_L231_I0.9991.00
23_I80_I0.9981.00
112_L117_L0.9961.00
108_F160_I0.9891.00
100_T159_N0.9881.00
215_L218_M0.9851.00
86_A263_L0.9831.00
60_L165_I0.9811.00
95_A127_V0.9801.00
115_K287_F0.9801.00
15_V231_I0.9751.00
269_G272_F0.9701.00
214_I218_M0.9691.00
32_P36_A0.9681.00
132_E268_T0.9651.00
113_H117_L0.9601.00
240_E244_V0.9521.00
128_L132_E0.9471.00
41_M190_F0.9451.00
92_V145_F0.9441.00
40_F185_P0.9391.00
92_V152_A0.9391.00
122_L281_G0.9351.00
100_T156_A0.9331.00
106_F109_G0.9311.00
26_G216_S0.9271.00
33_L74_A0.9271.00
21_V262_L0.9241.00
92_V148_T0.9241.00
192_A196_L0.9231.00
58_L184_I0.9161.00
34_M193_S0.9141.00
144_G148_T0.9121.00
103_L153_F0.9111.00
218_M221_A0.9101.00
103_L106_F0.9091.00
46_P222_F0.9061.00
191_V195_I0.9061.00
236_L240_E0.9011.00
239_Y244_V0.8981.00
192_A195_I0.8881.00
74_A78_C0.8861.00
65_G184_I0.8831.00
69_S158_G0.8751.00
141_A145_F0.8731.00
61_L64_Y0.8711.00
13_V17_G0.8651.00
13_V254_V0.8591.00
111_R115_K0.8561.00
91_L128_L0.8521.00
183_L187_I0.8501.00
86_A265_E0.8441.00
239_Y243_R0.8351.00
253_V275_A0.8331.00
48_I183_L0.8321.00
29_N210_D0.8301.00
139_H142_M0.8251.00
60_L65_G0.8241.00
210_D214_I0.8221.00
256_L267_L0.8181.00
58_L62_L0.8151.00
186_I190_F0.8151.00
125_F129_V0.8121.00
208_T211_M0.8111.00
260_A267_L0.8101.00
10_L254_V0.8041.00
67_T154_S0.8001.00
148_T152_A0.8001.00
127_V131_I0.7961.00
180_W184_I0.7951.00
111_R114_G0.7941.00
270_L273_L0.7941.00
51_V55_K0.7941.00
39_R225_T0.7931.00
21_V259_A0.7921.00
60_L164_K0.7901.00
184_I187_I0.7901.00
275_A279_M0.7881.00
235_L244_V0.7871.00
157_C161_F0.7841.00
129_V273_L0.7831.00
70_F74_A0.7811.00
58_L61_L0.7771.00
209_I214_I0.7741.00
13_V250_L0.7711.00
47_A226_I0.7671.00
125_F277_L0.7661.00
217_L221_A0.7651.00
19_N224_A0.7611.00
155_W159_N0.7531.00
172_P177_L0.7521.00
179_I183_L0.7501.00
44_A48_I0.7491.00
218_M222_F0.7481.00
250_L253_V0.7421.00
23_I27_L0.7411.00
118_A122_L0.7411.00
62_L66_L0.7401.00
98_F123_A0.7371.00
46_P49_F0.7301.00
72_Q154_S0.7281.00
34_M38_L0.7251.00
36_A189_F0.7251.00
273_L277_L0.7251.00
256_L275_A0.7211.00
102_M106_F0.7171.00
60_L63_G0.7141.00
167_S171_R0.7131.00
20_F76_L0.7121.00
31_P197_D0.7121.00
176_S180_W0.7111.00
38_L42_L0.7081.00
41_M45_F0.7061.00
105_A109_G0.7051.00
101_I116_Q0.7051.00
112_L116_Q0.7031.00
227_V231_I0.7021.00
100_T160_I0.7001.00
79_A144_G0.7001.00
95_A98_F0.6991.00
195_I198_G0.6961.00
178_V181_S0.6921.00
21_V86_A0.6901.00
25_V263_L0.6871.00
260_A266_R0.6841.00
184_I188_P0.6841.00
155_W232_W0.6831.00
61_L172_P0.6801.00
50_F175_M0.6781.00
174_V178_V0.6751.00
71_A75_F0.6721.00
230_G234_T0.6721.00
79_A89_A0.6711.00
246_P285_N0.6681.00
259_A263_L0.6681.00
9_A239_Y0.6681.00
267_L271_Q0.6671.00
58_L180_W0.6661.00
223_V227_V0.6661.00
67_T157_C0.6651.00
281_G285_N0.6641.00
9_A247_L0.6641.00
101_I105_A0.6641.00
280_T284_I0.6631.00
72_Q158_G0.6631.00
94_Q127_V0.6621.00
92_V149_L0.6621.00
84_M89_A0.6601.00
219_Y223_V0.6601.00
69_S229_Y0.6601.00
30_M35_L0.6591.00
196_L199_S0.6581.00
266_R270_L0.6551.00
172_P176_S0.6531.00
256_L260_A0.6521.00
216_S219_Y0.6521.00
99_F149_L0.6521.00
213_T216_S0.6511.00
20_F90_S0.6501.00
126_G281_G0.6441.00
122_L284_I0.6431.00
140_V143_L0.6421.00
110_E242_W0.6381.00
194_L197_D0.6361.00
187_I190_F0.6341.00
77_F81_N0.6291.00
58_L65_G0.6241.00
107_T157_C0.6201.00
94_Q97_A0.6191.00
282_L285_N0.6181.00
67_T161_F0.6151.00
271_Q275_A0.6141.00
39_R224_A0.6131.00
43_V226_I0.6101.00
139_H143_L0.6091.00
76_L89_A0.6081.00
183_L186_I0.6081.00
180_W183_L0.6081.00
194_L198_G0.6051.00
95_A152_A0.6051.00
257_A261_L0.6051.00
97_A249_L0.6001.00
116_Q240_E0.5991.00
15_V228_G0.5991.00
20_F24_K0.5951.00
185_P189_F0.5941.00
69_S181_S0.5921.00
108_F164_K0.5871.00
42_L46_P0.5851.00
76_L151_A0.5841.00
147_L150_A0.5821.00
46_P221_A0.5811.00
70_F192_A0.5801.00
158_G233_G0.5801.00
249_L282_L0.5751.00
11_L15_V0.5731.00
65_G70_F0.5691.00
210_D215_L0.5681.00
258_S261_L0.5641.00
268_T272_F0.5641.00
159_N245_A0.5641.00
38_L217_L0.5631.00
30_M34_M0.5621.00
45_F48_I0.5601.00
27_L80_I0.5591.00
33_L196_L0.5581.00
137_G140_V0.5561.00
69_S185_P0.5541.00
248_S251_V0.5491.00
209_I213_T0.5471.00
153_F157_C0.5471.00
178_V233_G0.5461.00
178_V182_A0.5451.00
10_L13_V0.5401.00
159_N232_W0.5401.00
177_L180_W0.5401.00
266_R271_Q0.5371.00
113_H288_G0.5351.00
91_L131_I0.5351.00
13_V251_V0.5321.00
57_P61_L0.5321.00
260_A272_F0.5301.00
158_G181_S0.5251.00
190_F194_L0.5251.00
108_F163_K0.5251.00
72_Q76_L0.5241.00
17_G251_V0.5241.00
162_N166_M0.5241.00
27_L81_N0.5241.00
235_L239_Y0.5241.00
138_Q141_A0.5221.00
131_I145_F0.5171.00
193_S197_D0.5161.00
102_M120_I0.5151.00
61_L177_L0.5131.00
133_D270_L0.5121.00
8_L11_L0.5111.00
231_I234_T0.5101.00
79_A84_M0.5101.00
42_L217_L0.5091.00
162_N236_L0.5091.00
284_I288_G0.5071.00
257_A260_A0.5051.00
75_F151_A0.5011.00
95_A123_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2i68A 2 0.2575 98.8 0.759 Contact Map
3b5dA 2 0.3378 98.3 0.791 Contact Map
4he8J 1 0.5217 20.7 0.934 Contact Map
2kluA 1 0.1472 19 0.936 Contact Map
2cfqA 1 0.5987 11.8 0.941 Contact Map
2m20A 2 0.1204 11.6 0.942 Contact Map
4n7wA 2 0.9298 9.7 0.944 Contact Map
1u7gA 3 0.7893 9.7 0.944 Contact Map
3l1lA 2 0.8829 8.4 0.945 Contact Map
2rddB 1 0.1104 6.5 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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