GREMLIN Database
PANB - 3-methyl-2-oxobutanoate hydroxymethyltransferase
UniProt: P31057 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11675
Length: 264 (261)
Sequences: 3106 (1884)
Seq/√Len: 116.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
18_R254_G3.4461.00
88_M101_A3.1231.00
235_A238_G2.9141.00
189_K193_E2.8811.00
123_V170_L2.8351.00
69_T83_A2.7811.00
189_K207_V2.6651.00
233_F250_E2.6481.00
21_T212_I2.6141.00
62_V99_N2.5821.00
171_E196_A2.4201.00
48_G60_V2.3061.00
168_L194_A2.2931.00
13_K209_D2.2811.00
171_E195_L2.1821.00
52_Q67_Y2.1311.00
134_C177_L2.1261.00
62_V89_A2.1001.00
95_Q98_E2.0941.00
17_K252_E2.0551.00
231_K261_H2.0461.00
231_K250_E2.0381.00
239_D242_A2.0281.00
12_Y19_F2.0231.00
20_A248_M1.9931.00
192_T199_V1.9901.00
38_L248_M1.9381.00
248_M252_E1.9131.00
161_D165_S1.9021.00
117_E165_S1.8981.00
11_K15_E1.8731.00
124_Q128_E1.8641.00
190_R194_A1.8281.00
64_D70_A1.8061.00
249_A253_S1.7581.00
73_R81_L1.7551.00
62_V103_V1.7331.00
185_V262_S1.7151.00
134_C176_Q1.7141.00
234_L239_D1.7091.00
242_A246_Q1.7051.00
69_T108_A1.6991.00
162_Q166_D1.6771.00
33_F41_M1.6691.00
85_L99_N1.6581.00
83_A103_V1.6511.00
33_F244_V1.6391.00
156_G159_A1.6261.00
34_A79_C1.6021.00
56_S59_P1.5951.00
135_G170_L1.5601.00
175_A197_I1.5411.00
258_G261_H1.5381.00
52_Q71_A1.5261.00
9_L40_V1.5251.00
40_V212_I1.4971.00
164_L190_R1.4851.00
226_I232_N1.4851.00
168_L171_E1.4831.00
12_Y17_K1.4801.00
250_E255_V1.4661.00
41_M76_A1.4661.00
29_F215_M1.4581.00
63_A102_T1.4501.00
206_N262_S1.4441.00
135_G175_A1.4381.00
69_T106_A1.4301.00
96_A122_T1.4281.00
232_N235_A1.4271.00
103_V106_A1.4121.00
178_L195_L1.3791.00
110_M176_Q1.3751.00
238_G242_A1.3701.00
85_L100_A1.3611.00
153_Q159_A1.3581.00
170_L175_A1.3211.00
171_E197_I1.3101.00
40_V134_C1.3081.00
21_T40_V1.2961.00
168_L195_L1.2820.99
83_A106_A1.2730.99
140_T147_F1.2730.99
66_A103_V1.2700.99
93_P122_T1.2670.99
219_F240_I1.2630.99
137_L163_L1.2610.99
125_M129_R1.2590.99
117_E166_D1.2520.99
22_I218_A1.2420.99
137_L180_L1.2410.99
109_N145_N1.2380.99
178_L199_V1.2270.99
135_G179_V1.2250.99
157_D161_D1.2100.99
3_P109_N1.1940.99
33_F215_M1.1940.99
82_L179_V1.1930.99
124_Q172_A1.1920.99
35_D241_R1.1910.99
70_A74_R1.1860.99
19_F210_G1.1720.99
10_Q14_Q1.1670.99
118_W162_Q1.1560.99
48_G54_H1.1520.99
90_Y113_I1.1430.99
4_T39_N1.1320.99
121_E124_Q1.1230.99
4_T8_L1.1230.99
20_A252_E1.1180.99
83_A108_A1.1170.99
199_V208_T1.1140.99
245_R249_A1.1120.99
82_L134_C1.1120.99
76_A81_L1.1060.98
95_Q99_N1.1060.98
180_L183_V1.1030.98
127_T175_A1.1010.98
54_H74_R1.1000.98
185_V207_V1.0970.98
54_H59_P1.0960.98
97_F125_M1.0960.98
241_R245_R1.0940.98
215_M219_F1.0930.98
34_A78_N1.0910.98
178_L197_I1.0740.98
192_T208_T1.0660.98
93_P125_M1.0620.98
7_S151_K1.0600.98
182_C263_F1.0580.98
92_T95_Q1.0550.98
258_G262_S1.0540.98
82_L110_M1.0500.98
219_F236_E1.0470.98
40_V82_L1.0370.98
154_G191_I1.0200.97
185_V206_N1.0090.97
20_A38_L1.0060.97
102_T106_A1.0060.97
47_L68_H1.0040.97
34_A77_P1.0040.97
31_K224_G0.9960.97
54_H64_D0.9870.97
120_V166_D0.9860.97
51_V216_H0.9820.97
118_W128_E0.9800.97
8_L11_K0.9770.97
139_L182_C0.9740.97
28_S221_I0.9720.97
165_S169_A0.9680.97
48_G64_D0.9590.96
4_T78_N0.9580.96
222_T225_H0.9560.96
188_A208_T0.9540.96
160_G187_L0.9510.96
85_L89_A0.9500.96
97_F126_L0.9500.96
63_A66_A0.9350.96
32_L244_V0.9340.96
28_S51_V0.9330.96
64_D67_Y0.9250.95
144_V149_G0.9230.95
90_Y96_A0.9220.95
192_T209_D0.9190.95
94_E98_E0.9160.95
246_Q249_A0.9160.95
206_N254_G0.9150.95
246_Q250_E0.9100.95
179_V197_I0.9100.95
166_D169_A0.9080.95
119_L122_T0.9020.95
59_P105_R0.9020.95
120_V169_A0.8970.95
91_A105_R0.8920.94
97_F129_R0.8910.94
147_F182_C0.8890.94
19_F39_N0.8850.94
190_R193_E0.8840.94
180_L191_I0.8810.94
22_I33_F0.8790.94
104_M132_P0.8770.94
80_L110_M0.8710.94
62_V65_I0.8710.94
127_T176_Q0.8690.94
237_T241_R0.8660.94
223_G237_T0.8660.94
73_R76_A0.8620.93
22_I38_L0.8620.93
47_L60_V0.8570.93
117_E162_Q0.8560.93
164_L168_L0.8510.93
117_E169_A0.8410.93
81_L109_N0.8390.92
184_P204_A0.8380.92
236_E240_I0.8360.92
66_A102_T0.8360.92
28_S216_H0.8330.92
213_L229_F0.8310.92
110_M134_C0.8280.92
154_G163_L0.8230.92
70_A73_R0.8230.92
31_K35_D0.8170.91
39_N252_E0.8170.91
233_F243_A0.8170.91
161_D190_R0.8140.91
67_Y71_A0.8120.91
89_A105_R0.8100.91
242_A245_R0.8090.91
164_L194_A0.8080.91
41_M81_L0.8070.91
52_Q64_D0.8070.91
93_P121_E0.7970.90
47_L52_Q0.7940.90
29_F216_H0.7890.90
66_A106_A0.7890.90
97_F101_A0.7870.90
234_L243_A0.7870.90
236_E239_D0.7810.90
12_Y252_E0.7810.90
61_T64_D0.7800.89
116_G120_V0.7730.89
182_C228_K0.7720.89
152_V228_K0.7720.89
4_T9_L0.7650.89
39_N210_G0.7600.88
170_L178_L0.7590.88
9_L176_Q0.7550.88
32_L237_T0.7540.88
22_I244_V0.7520.88
19_F40_V0.7480.87
250_E253_S0.7470.87
73_R109_N0.7450.87
229_F261_H0.7410.87
20_A39_N0.7400.87
235_A239_D0.7370.87
62_V66_A0.7370.87
65_I83_A0.7360.87
184_P187_L0.7320.86
147_F153_Q0.7310.86
256_Y263_F0.7300.86
94_E125_M0.7290.86
92_T122_T0.7290.86
233_F255_V0.7280.86
122_T125_M0.7260.86
27_Y31_K0.7250.86
215_M244_V0.7200.85
175_A178_L0.7170.85
36_E241_R0.7140.85
22_I215_M0.7130.85
149_G153_Q0.7070.84
91_A96_A0.7060.84
193_E207_V0.7040.84
29_F240_I0.7030.84
12_Y39_N0.6970.83
168_L172_A0.6950.83
31_K237_T0.6920.83
152_V263_F0.6900.83
66_A69_T0.6900.83
137_L178_L0.6890.83
88_M97_F0.6860.83
10_Q196_A0.6840.82
101_A105_R0.6840.82
117_E120_V0.6840.82
47_L67_Y0.6810.82
159_A162_Q0.6780.82
169_A172_A0.6770.82
227_P230_A0.6740.81
165_S168_L0.6720.81
231_K255_V0.6710.81
73_R106_A0.6710.81
34_A76_A0.6710.81
216_H221_I0.6690.81
160_G190_R0.6690.81
93_P96_A0.6690.81
139_L150_Y0.6680.81
58_L105_R0.6650.81
135_G178_L0.6650.81
169_A173_A0.6610.80
127_T146_I0.6590.80
152_V182_C0.6510.79
182_C222_T0.6480.79
90_Y99_N0.6470.79
122_T126_L0.6460.79
70_A106_A0.6440.78
111_V133_V0.6430.78
85_L90_Y0.6390.78
180_L199_V0.6380.78
25_Y51_V0.6370.78
220_G226_I0.6330.77
11_K14_Q0.6320.77
185_V259_E0.6290.77
21_T30_A0.6280.77
113_I126_L0.6270.77
40_V80_L0.6260.76
101_A131_V0.6260.76
231_K258_G0.6260.76
5_T139_L0.6250.76
55_D59_P0.6230.76
191_I194_A0.6210.76
6_I176_Q0.6200.76
115_G166_D0.6190.76
26_D29_F0.6190.76
163_L187_L0.6160.75
48_G61_T0.6160.75
93_P97_F0.6130.75
195_L199_V0.6130.75
66_A70_A0.6130.75
6_I10_Q0.6120.75
73_R77_P0.6090.74
29_F219_F0.6070.74
49_M227_P0.6060.74
30_A76_A0.6030.74
85_L103_V0.6020.74
220_G232_N0.6020.74
146_I176_Q0.6000.73
120_V173_A0.5990.73
150_Y182_C0.5980.73
180_L188_A0.5970.73
30_A41_M0.5940.73
137_L154_G0.5930.72
36_E245_R0.5910.72
202_I212_I0.5900.72
51_V71_A0.5890.72
233_F236_E0.5880.72
179_V195_L0.5880.72
58_L143_S0.5850.71
19_F200_I0.5840.71
240_I244_V0.5830.71
9_L12_Y0.5810.71
127_T173_A0.5810.71
99_N103_V0.5800.71
222_T226_I0.5800.71
8_L12_Y0.5780.71
144_V150_Y0.5780.71
160_G184_P0.5760.70
150_Y227_P0.5750.70
259_E262_S0.5730.70
130_A146_I0.5730.70
121_E128_E0.5730.70
82_L177_L0.5720.70
100_A131_V0.5710.70
200_I210_G0.5710.70
140_T143_S0.5620.68
153_Q263_F0.5610.68
168_L196_A0.5590.68
72_V81_L0.5590.68
183_V199_V0.5570.68
87_F130_A0.5570.68
126_L129_R0.5540.67
9_L19_F0.5530.67
113_I119_L0.5510.67
8_L49_M0.5510.67
199_V209_D0.5510.67
58_L144_V0.5500.67
40_V177_L0.5490.67
27_Y75_G0.5470.66
97_F122_T0.5440.66
116_G122_T0.5420.66
123_V135_G0.5410.66
104_M126_L0.5400.65
218_A230_A0.5380.65
123_V175_A0.5370.65
21_T82_L0.5290.64
24_A43_V0.5280.64
153_Q184_P0.5270.64
12_Y15_E0.5260.63
13_K18_R0.5260.63
60_V64_D0.5260.63
62_V102_T0.5240.63
183_V187_L0.5230.63
20_A210_G0.5210.63
230_A263_F0.5210.63
26_D216_H0.5190.62
35_D245_R0.5180.62
43_V83_A0.5180.62
24_A41_M0.5150.62
186_E190_R0.5150.62
24_A30_A0.5150.62
31_K223_G0.5140.62
250_E261_H0.5140.62
192_T207_V0.5140.62
6_I80_L0.5130.61
58_L148_G0.5110.61
98_E101_A0.5110.61
24_A33_F0.5100.61
152_V184_P0.5070.61
9_L212_I0.5070.61
215_M221_I0.5050.60
249_A255_V0.5050.60
188_A192_T0.5040.60
219_F244_V0.5020.60
19_F198_P0.5010.60
233_F246_Q0.5000.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1m3uA 4 0.9924 100 0.207 Contact Map
1o66A 3 0.9432 100 0.208 Contact Map
1oy0A 4 0.9318 100 0.225 Contact Map
3vavA 4 0.9962 100 0.229 Contact Map
4lsbA 2 0.9053 100 0.529 Contact Map
4mg4A 4 0.8826 100 0.597 Contact Map
3lyeA 4 0.9015 100 0.607 Contact Map
2qiwA 2 0.8864 100 0.613 Contact Map
3eooA 4 0.9091 100 0.614 Contact Map
1zlpA 4 0.8939 100 0.619 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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