GREMLIN Database
DKGB - 2,5-diketo-D-gluconic acid reductase B
UniProt: P30863 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11648
Length: 267 (244)
Sequences: 32465 (20991)
Seq/√Len: 1343.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
24_T229_E3.1171.00
80_K122_L2.8751.00
50_A87_R2.7091.00
199_H243_D2.6411.00
15_D47_Q2.6181.00
240_D243_D2.4521.00
19_I51_E2.3931.00
79_L117_A2.3901.00
76_I116_E2.3761.00
76_I120_Q2.2731.00
24_T28_L2.2681.00
198_K246_A2.0761.00
115_L119_K2.0441.00
19_I47_Q2.0301.00
81_E85_K1.9721.00
167_W171_H1.9701.00
193_A197_A1.9641.00
90_Y124_R1.9581.00
213_G244_K1.9481.00
116_E120_Q1.9381.00
225_R229_E1.8761.00
55_P58_E1.8641.00
241_A245_K1.8601.00
198_K243_D1.8561.00
24_T225_R1.8421.00
168_A175_I1.8351.00
72_K116_E1.8001.00
194_R246_A1.8001.00
7_G32_A1.7901.00
201_A237_L1.7701.00
28_L229_E1.7241.00
25_A30_Y1.6831.00
95_L150_T1.6821.00
214_E248_A1.6631.00
116_E119_K1.6351.00
72_K76_I1.6241.00
14_K17_V1.6161.00
174_H217_S1.6101.00
137_E141_A1.6071.00
28_L232_L1.5881.00
61_I88_T1.5841.00
19_I23_I1.5831.00
43_A85_K1.5781.00
24_T27_E1.5751.00
191_V194_R1.5741.00
65_I94_T1.5721.00
137_E167_W1.5681.00
23_I51_E1.5631.00
19_I48_A1.5471.00
76_I80_K1.5451.00
202_T230_S1.5441.00
245_K249_A1.5421.00
209_A237_L1.5321.00
63_T91_V1.5291.00
63_T82_S1.5261.00
65_I75_L1.5201.00
233_K236_N1.5171.00
113_A116_E1.5171.00
79_L94_T1.5041.00
62_T95_L1.4871.00
15_D19_I1.4631.00
10_T33_I1.4481.00
77_P81_E1.4371.00
37_Q78_S1.4321.00
17_V20_S1.4021.00
26_L59_L1.3971.00
163_K166_A1.3971.00
201_A205_Q1.3871.00
132_T135_L1.3791.00
140_I171_H1.3701.00
166_A170_Q1.3651.00
80_K84_Q1.3641.00
169_K215_G1.3631.00
125_E149_A1.3531.00
22_V48_A1.3511.00
95_L176_T1.3371.00
209_A213_G1.3151.00
91_V123_T1.3041.00
229_E233_K1.3001.00
153_I175_I1.2991.00
222_S227_N1.2921.00
246_A249_A1.2681.00
23_I27_E1.2631.00
81_E84_Q1.2581.00
72_K112_Q1.2541.00
194_R198_K1.2501.00
20_S24_T1.2501.00
201_A206_V1.2471.00
140_I167_W1.2471.00
60_Y93_L1.2341.00
117_A123_T1.2151.00
56_R88_T1.2081.00
191_V246_A1.1991.00
123_T126_I1.1991.00
72_K113_A1.1961.00
76_I113_A1.1921.00
86_L91_V1.1891.00
133_I167_W1.1791.00
193_A203_P1.1761.00
46_G87_R1.1731.00
165_V169_K1.1641.00
222_S228_L1.1641.00
42_E82_S1.1641.00
47_Q87_R1.1611.00
202_T205_Q1.1531.00
108_E142_A1.1501.00
99_P135_L1.1501.00
118_K149_A1.1441.00
93_L150_T1.1391.00
155_L214_E1.1381.00
186_A189_D1.1341.00
16_D20_S1.1241.00
56_R90_Y1.1221.00
119_K144_G1.1191.00
74_K77_P1.1041.00
43_A47_Q1.0991.00
73_D77_P1.0981.00
15_D44_A1.0961.00
33_I45_V1.0811.00
137_E171_H1.0791.00
71_S74_K1.0781.00
32_A62_T1.0771.00
32_A176_T1.0771.00
154_E159_L1.0761.00
115_L118_K1.0721.00
194_R197_A1.0691.00
114_L126_I1.0661.00
226_K229_E1.0631.00
79_L91_V1.0601.00
23_I48_A1.0521.00
137_E170_Q1.0501.00
10_T45_V1.0491.00
190_E194_R1.0421.00
80_K120_Q1.0401.00
117_A122_L1.0391.00
96_I114_L1.0391.00
211_A218_V1.0231.00
70_L96_I1.0171.00
31_R60_Y1.0161.00
21_S228_L1.0121.00
195_I206_V1.0121.00
20_S225_R1.0111.00
109_E112_Q1.0051.00
4_P31_R0.9971.00
205_Q233_K0.9961.00
133_I137_E0.9861.00
160_Q251_D0.9851.00
190_E193_A0.9771.00
212_M234_A0.9721.00
157_P207_I0.9701.00
157_P247_I0.9611.00
5_A235_Q0.9601.00
138_K142_A0.9571.00
42_E85_K0.9561.00
158_Y189_D0.9461.00
192_I203_P0.9411.00
92_D124_R0.9391.00
37_Q74_K0.9371.00
176_T217_S0.9331.00
4_P30_Y0.9321.00
150_T176_T0.9301.00
47_Q51_E0.9261.00
162_R166_A0.9241.00
5_A212_M0.9191.00
166_A169_K0.9121.00
76_I79_L0.9101.00
228_L232_L0.9081.00
128_I148_I0.9071.00
112_Q116_E0.9051.00
69_N74_K0.9031.00
49_I59_L0.9021.00
230_S233_K0.8961.00
205_Q230_S0.8951.00
20_S23_I0.8811.00
22_V33_I0.8811.00
118_K145_A0.8801.00
108_E138_K0.8781.00
113_A117_A0.8781.00
37_Q69_N0.8781.00
118_K125_E0.8741.00
117_A120_Q0.8731.00
77_P80_K0.8701.00
213_G238_Q0.8691.00
226_K230_S0.8691.00
26_L55_P0.8641.00
224_K227_N0.8631.00
151_N168_A0.8601.00
115_L144_G0.8571.00
245_K248_A0.8571.00
128_I136_M0.8511.00
167_W170_Q0.8431.00
62_T176_T0.8431.00
43_A84_Q0.8391.00
37_Q85_K0.8381.00
112_Q115_L0.8371.00
242_E246_A0.8341.00
76_I117_A0.8251.00
115_L143_V0.8231.00
72_K109_E0.8181.00
126_I148_I0.8171.00
242_E245_K0.8161.00
208_L234_A0.8141.00
130_N178_Y0.8051.00
138_K141_A0.8041.00
214_E244_K0.8041.00
195_I199_H0.7991.00
8_L25_A0.7951.00
136_M173_I0.7941.00
51_E55_P0.7841.00
207_I211_A0.7821.00
115_L126_I0.7801.00
160_Q248_A0.7771.00
141_A145_A0.7721.00
62_T93_L0.7711.00
144_G147_N0.7701.00
37_Q81_E0.7661.00
209_A234_A0.7661.00
28_L233_K0.7611.00
155_L165_V0.7601.00
223_T227_N0.7581.00
180_T220_P0.7511.00
35_T45_V0.7461.00
213_G241_A0.7461.00
165_V175_I0.7411.00
63_T94_T0.7361.00
198_K240_D0.7351.00
140_I147_N0.7341.00
24_T228_L0.7321.00
199_H240_D0.7301.00
177_S180_T0.7301.00
83_L122_L0.7281.00
133_I164_V0.7281.00
18_V44_A0.7251.00
23_I55_P0.7221.00
16_D19_I0.7211.00
10_T41_N0.7191.00
98_W130_N0.7171.00
164_V168_A0.7121.00
30_Y232_L0.7061.00
204_A230_S0.7041.00
82_S94_T0.6971.00
191_V250_L0.6971.00
78_S81_E0.6951.00
26_L33_I0.6931.00
197_A200_N0.6911.00
83_L88_T0.6901.00
75_L117_A0.6891.00
165_V214_E0.6871.00
177_S218_V0.6821.00
79_L122_L0.6801.00
70_L110_F0.6741.00
174_H216_Y0.6741.00
210_W248_A0.6731.00
106_S109_E0.6711.00
111_M139_A0.6691.00
128_I131_F0.6691.00
95_L127_G0.6681.00
111_M148_I0.6681.00
196_A206_V0.6671.00
7_G220_P0.6641.00
21_S225_R0.6631.00
133_I163_K0.6621.00
111_M143_V0.6571.00
211_A216_Y0.6561.00
8_L13_L0.6551.00
6_F212_M0.6491.00
111_M126_I0.6481.00
195_I243_D0.6471.00
12_R183_Y0.6461.00
201_A233_K0.6461.00
239_L244_K0.6461.00
128_I151_N0.6421.00
206_V239_L0.6411.00
7_G217_S0.6401.00
180_T211_A0.6401.00
201_A236_N0.6381.00
199_H206_V0.6351.00
31_R58_E0.6331.00
75_L110_F0.6331.00
155_L211_A0.6251.00
168_A173_I0.6241.00
227_N230_S0.6141.00
57_H90_Y0.6141.00
91_V94_T0.6131.00
118_K123_T0.6121.00
3_I217_S0.6061.00
114_L118_K0.6041.00
22_V26_L0.6041.00
28_L228_L0.6031.00
34_D152_Q0.6031.00
11_F179_M0.6031.00
55_P59_L0.6021.00
13_L18_V0.5981.00
192_I247_I0.5951.00
110_F113_A0.5951.00
27_E225_R0.5951.00
137_E140_I0.5901.00
140_I143_V0.5861.00
67_I70_L0.5851.00
75_L113_A0.5851.00
155_L175_I0.5841.00
26_L31_R0.5841.00
107_V142_A0.5841.00
201_A209_A0.5841.00
83_L90_Y0.5811.00
17_V225_R0.5801.00
158_Y185_K0.5791.00
79_L83_L0.5771.00
179_M183_Y0.5771.00
192_I207_I0.5731.00
14_K18_V0.5721.00
206_V247_I0.5721.00
26_L58_E0.5711.00
222_S231_N0.5701.00
134_P137_E0.5681.00
195_I246_A0.5681.00
243_D246_A0.5671.00
105_V109_E0.5661.00
54_V59_L0.5641.00
135_L138_K0.5611.00
206_V243_D0.5611.00
155_L160_Q0.5581.00
38_I66_W0.5571.00
239_L243_D0.5571.00
21_S222_S0.5541.00
155_L216_Y0.5531.00
106_S110_F0.5511.00
83_L91_V0.5471.00
71_S110_F0.5421.00
25_A33_I0.5361.00
76_I122_L0.5351.00
65_I70_L0.5321.00
8_L21_S0.5321.00
192_I196_A0.5321.00
6_F235_Q0.5321.00
108_E112_Q0.5311.00
115_L145_A0.5311.00
196_A199_H0.5301.00
94_T114_L0.5291.00
195_I198_K0.5281.00
35_T41_N0.5281.00
206_V237_L0.5281.00
65_I78_S0.5251.00
107_V110_F0.5241.00
94_T123_T0.5241.00
196_A203_P0.5241.00
83_L89_D0.5231.00
32_A60_Y0.5211.00
212_M218_V0.5201.00
49_I55_P0.5201.00
33_I49_I0.5191.00
13_L21_S0.5191.00
22_V45_V0.5181.00
99_P131_F0.5181.00
100_S105_V0.5171.00
205_Q237_L0.5161.00
143_V148_I0.5131.00
47_Q50_A0.5131.00
72_K75_L0.5121.00
140_I173_I0.5101.00
107_V138_K0.5071.00
45_V48_A0.5031.00
100_S104_E0.5031.00
96_I110_F0.5031.00
244_K248_A0.5031.00
161_N166_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3up8A 1 0.9888 100 0.06 Contact Map
4otkA 1 0.9813 100 0.08 Contact Map
2wzmA 1 0.985 100 0.081 Contact Map
1hw6A 1 0.9176 100 0.084 Contact Map
3o0kA 1 0.9326 100 0.085 Contact Map
1mi3A 3 0.9963 100 0.085 Contact Map
1zgdA 1 0.9963 100 0.087 Contact Map
4q3mA 2 0.9775 100 0.088 Contact Map
4wevX 1 0.9513 100 0.088 Contact Map
1us0A 1 0.9963 100 0.09 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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