BASS - ECOLI - GREMLIN Contacts

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BASS - Sensor protein BasS

UniProt:	P30844	Sequences: MHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQIQLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQKELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTPLAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLEDVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGSNIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQLHHGQFFLQNRQETSGTRAWVRLKKDQYVANQI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG11614
Length:	363 (290)
Sequences:	57518 (45799)
Seq/√Len:	2689.4

ECOLI - BASS TORS BARA RCSD GLRK BAES RCSC PHOQ CPXA YEDV CUSS RSTB ENVZ CREC QSEC ARCB ATOS EVGS ZRAS KDPD PHOR DCUS NARQ DPIB NARX YPDA NTRB UHPB
BACSU - YCLK YVRG YKOH YVCQ CSSS YYCG RESE YXDK KINB PHOR BCES YCBM KINE KINA GLNK KIND CITS KINC DCTS MALK YBDK YRKQ YESM LYTS LIAS YWPD NATK DEGS COMP YDFH YFIJ YXJM YHCY
HALSA - Q9HQ15 Q9HQG5 Q9HQE9 Q9HQ16 Q9HRE4 O51970 Q9HR10 Q9HRG1 Q9HNM2 Q9HNA6 BAT Q9HQB9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
291_D	295_G	3.482	1.00
290_E	348_R	3.361	1.00
211_V	249_G	3.081	1.00
326_S	330_Q	3.036	1.00
337_F	350_W	2.942	1.00
165_L	179_L	2.789	1.00
301_C	340_N	2.637	1.00
288_A	348_R	2.597	1.00
249_G	332_H	2.582	1.00
269_S	292_E	2.344	1.00
278_K	288_A	2.340	1.00
284_G	352_R	2.324	1.00
288_A	350_W	2.288	1.00
257_L	329_T	2.277	1.00
169_A	180_V	2.264	1.00
237_L	276_M	2.252	1.00
297_D	300_K	2.250	1.00
286_V	352_R	2.243	1.00
338_L	349_A	2.234	1.00
210_H	248_Q	2.230	1.00
341_R	345_S	2.208	1.00
325_V	336_F	2.205	1.00
162_R	184_D	2.197	1.00
281_E	355_K	2.190	1.00
286_V	350_W	2.161	1.00
280_Q	286_V	2.151	1.00
276_M	290_E	2.142	1.00
237_L	274_N	2.139	1.00
239_P	278_K	2.112	1.00
305_S	336_F	2.105	1.00
274_N	292_E	2.095	1.00
169_A	177_A	2.091	1.00
301_C	338_L	2.065	1.00
278_K	290_E	2.048	1.00
329_T	334_G	2.048	1.00
210_H	246_T	2.014	1.00
339_Q	348_R	2.009	1.00
280_Q	288_A	1.977	1.00
339_Q	350_W	1.945	1.00
296_I	304_L	1.909	1.00
209_Q	251_A	1.908	1.00
162_R	183_L	1.899	1.00
220_P	255_R	1.894	1.00
340_N	347_T	1.876	1.00
303_E	306_K	1.824	1.00
329_T	351_V	1.798	1.00
212_K	216_D	1.790	1.00
212_K	215_E	1.770	1.00
335_Q	352_R	1.767	1.00
235_T	274_N	1.766	1.00
273_S	292_E	1.760	1.00
300_K	303_E	1.730	1.00
298_E	301_C	1.724	1.00
257_L	328_I	1.710	1.00
225_L	259_R	1.703	1.00
239_P	276_M	1.702	1.00
162_R	187_M	1.683	1.00
249_G	254_L	1.677	1.00
341_R	344_T	1.660	1.00
263_E	267_R	1.636	1.00
269_S	273_S	1.623	1.00
296_I	300_K	1.610	1.00
166_E	180_V	1.600	1.00
118_A	123_E	1.592	1.00
187_M	191_S	1.586	1.00
229_L	266_H	1.575	1.00
303_E	309_V	1.565	1.00
120_L	123_E	1.557	1.00
104_A	109_N	1.551	1.00
330_Q	335_Q	1.547	1.00
257_L	325_V	1.546	1.00
300_K	309_V	1.539	1.00
296_I	347_T	1.538	1.00
208_Y	248_Q	1.531	1.00
153_E	256_M	1.526	1.00
161_V	183_L	1.521	1.00
337_F	352_R	1.488	1.00
328_I	332_H	1.477	1.00
145_L	149_D	1.446	1.00
141_D	145_L	1.443	1.00
165_L	180_V	1.432	1.00
305_S	338_L	1.426	1.00
300_K	311_M	1.422	1.00
258_L	287_M	1.413	1.00
132_L	136_L	1.377	1.00
260_N	325_V	1.359	1.00
119_T	123_E	1.356	1.00
287_M	351_V	1.347	1.00
253_L	256_M	1.339	1.00
143_E	147_T	1.316	1.00
276_M	292_E	1.311	1.00
291_D	346_G	1.305	1.00
140_L	143_E	1.286	1.00
212_K	244_D	1.285	1.00
250_D	253_L	1.284	1.00
340_N	344_T	1.283	1.00
130_N	133_V	1.277	1.00
161_V	179_L	1.266	1.00
133_V	136_L	1.264	1.00
226_S	230_D	1.233	1.00
180_V	184_D	1.232	1.00
84_C	88_V	1.229	1.00
340_N	345_S	1.218	1.00
215_E	219_L	1.207	1.00
149_D	307_A	1.203	1.00
257_L	351_V	1.198	1.00
168_L	176_V	1.198	1.00
220_P	251_A	1.195	1.00
122_I	126_V	1.182	1.00
285_A	355_K	1.171	1.00
297_D	342_Q	1.167	1.00
224_E	255_R	1.163	1.00
329_T	336_F	1.145	1.00
212_K	246_T	1.145	1.00
115_I	118_A	1.134	1.00
165_L	169_A	1.130	1.00
165_L	183_L	1.127	1.00
215_E	279_L	1.117	1.00
305_S	349_A	1.115	1.00
162_R	180_V	1.109	1.00
301_C	305_S	1.103	1.00
334_G	353_L	1.087	1.00
139_T	143_E	1.083	1.00
105_R	133_V	1.078	1.00
306_K	309_V	1.073	1.00
160_G	164_H	1.069	1.00
137_T	141_D	1.067	1.00
327_R	330_Q	1.066	1.00
304_L	338_L	1.062	1.00
253_L	328_I	1.059	1.00
164_H	178_P	1.057	1.00
251_A	255_R	1.057	1.00
160_G	182_R	1.057	1.00
253_L	331_L	1.053	1.00
102_L	106_T	1.042	1.00
206_G	250_D	1.041	1.00
102_L	105_R	1.026	1.00
310_R	315_Y	1.022	1.00
254_L	332_H	1.021	1.00
260_N	263_E	1.019	1.00
219_L	238_L	1.018	1.00
208_Y	332_H	1.005	1.00
188_E	252_T	1.004	1.00
338_L	347_T	1.001	1.00
222_Y	238_L	0.997	1.00
217_V	221_S	0.993	1.00
342_Q	345_S	0.993	1.00
138_S	142_N	0.993	1.00
146_F	150_V	0.990	1.00
228_M	231_Q	0.980	1.00
126_V	130_N	0.979	1.00
268_Y	310_R	0.975	1.00
221_S	259_R	0.975	1.00
145_L	307_A	0.972	1.00
269_S	275_I	0.968	1.00
115_I	119_T	0.966	1.00
118_A	122_I	0.966	1.00
119_T	122_I	0.963	1.00
290_E	341_R	0.958	1.00
291_D	347_T	0.958	1.00
99_Q	103_E	0.956	1.00
334_G	351_V	0.946	1.00
217_V	249_G	0.945	1.00
245_I	285_A	0.941	1.00
81_L	85_Y	0.940	1.00
112_P	115_I	0.939	1.00
67_A	71_V	0.935	1.00
118_A	126_V	0.933	1.00
310_R	317_G	0.932	1.00
241_S	244_D	0.930	1.00
305_S	326_S	0.924	1.00
215_E	244_D	0.921	1.00
122_I	125_V	0.919	1.00
177_A	181_A	0.919	1.00
114_A	118_A	0.918	1.00
163_L	167_L	0.917	1.00
221_S	225_L	0.916	1.00
221_S	255_R	0.911	1.00
88_V	92_T	0.907	1.00
99_Q	124_A	0.906	1.00
218_I	279_L	0.899	1.00
284_G	335_Q	0.887	1.00
165_L	176_V	0.887	1.00
182_R	185_Q	0.872	1.00
207_N	250_D	0.872	1.00
191_S	195_Q	0.869	1.00
131_D	135_R	0.869	1.00
123_E	126_V	0.865	1.00
211_V	217_V	0.862	1.00
228_M	263_E	0.858	1.00
296_I	302_G	0.857	1.00
295_G	347_T	0.855	1.00
91_I	94_P	0.854	1.00
155_R	191_S	0.850	1.00
181_A	220_P	0.845	1.00
241_S	279_L	0.842	1.00
268_Y	294_P	0.842	1.00
238_L	241_S	0.832	1.00
178_P	182_R	0.825	1.00
329_T	353_L	0.823	1.00
258_L	289_V	0.820	1.00
140_L	144_R	0.820	1.00
304_L	349_A	0.816	1.00
192_Q	253_L	0.816	1.00
129_L	132_L	0.814	1.00
258_L	262_V	0.809	1.00
298_E	340_N	0.809	1.00
89_R	93_R	0.807	1.00
265_A	275_I	0.805	1.00
179_L	183_L	0.802	1.00
162_R	166_E	0.798	1.00
158_L	190_V	0.798	1.00
252_T	255_R	0.796	1.00
123_E	127_S	0.794	1.00
222_Y	236_L	0.794	1.00
225_L	262_V	0.787	1.00
195_Q	256_M	0.786	1.00
208_Y	249_G	0.784	1.00
261_L	349_A	0.783	1.00
327_R	331_L	0.780	1.00
224_E	259_R	0.776	1.00
216_D	220_P	0.776	1.00
211_V	251_A	0.774	1.00
196_L	323_S	0.773	1.00
296_I	338_L	0.766	1.00
212_K	245_I	0.766	1.00
82_F	86_Q	0.766	1.00
259_R	263_E	0.765	1.00
296_I	301_C	0.765	1.00
154_L	186_M	0.765	1.00
270_P	273_S	0.761	1.00
183_L	187_M	0.754	1.00
128_A	131_D	0.753	1.00
178_P	181_A	0.747	1.00
245_I	281_E	0.742	1.00
219_L	223_D	0.740	1.00
167_L	171_T	0.734	1.00
160_G	163_L	0.733	1.00
144_R	200_G	0.733	1.00
129_L	133_V	0.732	1.00
218_I	258_L	0.731	1.00
213_L	287_M	0.730	1.00
125_V	129_L	0.728	1.00
208_Y	333_H	0.727	1.00
153_E	157_P	0.725	1.00
261_L	336_F	0.721	1.00
130_N	134_S	0.721	1.00
96_A	99_Q	0.719	1.00
288_A	339_Q	0.717	1.00
255_R	259_R	0.716	1.00
99_Q	128_A	0.714	1.00
320_L	324_I	0.713	1.00
119_T	126_V	0.708	1.00
296_I	340_N	0.705	1.00
192_Q	256_M	0.705	1.00
156_T	160_G	0.705	1.00
225_L	263_E	0.704	1.00
337_F	351_V	0.703	1.00
229_L	275_I	0.701	1.00
261_L	325_V	0.701	1.00
256_M	259_R	0.701	1.00
86_Q	90_R	0.700	1.00
261_L	351_V	0.700	1.00
94_P	98_L	0.700	1.00
215_E	243_A	0.698	1.00
118_A	125_V	0.698	1.00
206_G	253_L	0.695	1.00
256_M	328_I	0.690	1.00
85_Y	89_R	0.689	1.00
103_E	124_A	0.682	1.00
169_A	176_V	0.682	1.00
206_G	331_L	0.681	1.00
222_Y	226_S	0.680	1.00
138_S	141_D	0.680	1.00
242_A	245_I	0.680	1.00
325_V	329_T	0.679	1.00
63_R	67_A	0.678	1.00
229_L	236_L	0.677	1.00
233_Q	272_G	0.677	1.00
257_L	332_H	0.677	1.00
145_L	327_R	0.675	1.00
185_Q	255_R	0.675	1.00
297_D	311_M	0.671	1.00
172_H	175_D	0.669	1.00
88_V	91_I	0.668	1.00
334_G	354_K	0.667	1.00
283_D	355_K	0.666	1.00
177_A	180_V	0.659	1.00
223_D	226_S	0.659	1.00
267_R	315_Y	0.659	1.00
232_R	266_H	0.656	1.00
184_D	187_M	0.655	1.00
298_E	341_R	0.651	1.00
106_T	109_N	0.649	1.00
230_D	233_Q	0.649	1.00
182_R	224_E	0.649	1.00
141_D	144_R	0.648	1.00
188_E	192_Q	0.647	1.00
301_C	339_Q	0.646	1.00
113_I	133_V	0.645	1.00
213_L	285_A	0.642	1.00
66_V	70_I	0.641	1.00
211_V	248_Q	0.641	1.00
221_S	262_V	0.640	1.00
301_C	349_A	0.637	1.00
227_T	230_D	0.637	1.00
164_H	182_R	0.636	1.00
142_N	145_L	0.635	1.00
112_P	118_A	0.634	1.00
102_L	128_A	0.633	1.00
215_E	241_S	0.633	1.00
291_D	294_P	0.632	1.00
277_I	289_V	0.631	1.00
103_E	107_A	0.631	1.00
225_L	266_H	0.628	1.00
137_T	140_L	0.628	1.00
286_V	337_F	0.627	1.00
326_S	335_Q	0.626	1.00
149_D	153_E	0.625	1.00
290_E	344_T	0.623	1.00
229_L	234_Q	0.622	1.00
298_E	302_G	0.621	1.00
90_R	93_R	0.619	1.00
116_H	123_E	0.618	1.00
195_Q	253_L	0.618	1.00
159_A	163_L	0.618	1.00
152_H	260_N	0.617	1.00
194_L	197_A	0.616	1.00
147_T	150_V	0.615	1.00
218_I	277_I	0.612	1.00
243_A	281_E	0.612	1.00
102_L	129_L	0.612	1.00
147_T	151_A	0.608	1.00
98_L	102_L	0.608	1.00
234_Q	238_L	0.608	1.00
262_V	275_I	0.605	1.00
124_A	127_S	0.605	1.00
296_I	349_A	0.603	1.00
234_Q	266_H	0.599	1.00
150_V	193_L	0.599	1.00
103_E	128_A	0.596	1.00
139_T	142_N	0.595	1.00
155_R	187_M	0.595	1.00
233_Q	271_Q	0.594	1.00
116_H	120_L	0.593	1.00
68_S	71_V	0.592	1.00
216_D	251_A	0.590	1.00
295_G	346_G	0.588	1.00
114_A	126_V	0.588	1.00
106_T	131_D	0.587	1.00
70_I	74_V	0.586	1.00
112_P	119_T	0.584	1.00
234_Q	275_I	0.583	1.00
105_R	108_D	0.581	1.00
267_R	317_G	0.581	1.00
221_S	224_E	0.581	1.00
234_Q	269_S	0.579	1.00
217_V	254_L	0.578	1.00
307_A	327_R	0.574	1.00
126_V	129_L	0.574	1.00
105_R	132_L	0.573	1.00
160_G	178_P	0.571	1.00
113_I	134_S	0.570	1.00
200_G	327_R	0.567	1.00
113_I	118_A	0.565	1.00
294_P	315_Y	0.563	1.00
133_V	137_T	0.560	1.00
119_T	125_V	0.558	1.00
125_V	128_A	0.558	1.00
67_A	70_I	0.557	1.00
114_A	130_N	0.556	1.00
222_Y	277_I	0.556	1.00
310_R	316_G	0.555	1.00
228_M	232_R	0.555	1.00
104_A	108_D	0.554	1.00
239_P	280_Q	0.553	1.00
154_L	190_V	0.553	1.00
262_V	277_I	0.552	1.00
261_L	329_T	0.551	1.00
176_V	180_V	0.550	1.00
275_I	289_V	0.550	1.00
229_L	232_R	0.549	1.00
241_S	280_Q	0.548	1.00
153_E	260_N	0.547	1.00
164_H	179_L	0.545	1.00
245_I	279_L	0.545	1.00
232_R	271_Q	0.543	1.00
100_K	104_A	0.543	1.00
109_N	112_P	0.543	1.00
63_R	66_V	0.542	1.00
223_D	227_T	0.542	1.00
97_E	101_E	0.541	1.00
194_L	198_R	0.540	1.00
62_I	66_V	0.540	1.00
336_F	349_A	0.540	1.00
153_E	324_I	0.539	1.00
279_L	285_A	0.538	1.00
74_V	78_S	0.536	1.00
269_S	272_G	0.536	1.00
200_G	307_A	0.536	1.00
146_F	153_E	0.534	1.00
227_T	231_Q	0.533	1.00
265_A	289_V	0.532	1.00
304_L	323_S	0.532	1.00
166_E	170_K	0.531	1.00
101_E	104_A	0.530	1.00
262_V	266_H	0.530	1.00
124_A	128_A	0.528	1.00
300_K	312_D	0.527	1.00
297_D	301_C	0.526	1.00
247_V	285_A	0.525	1.00
135_R	138_S	0.523	1.00
235_T	238_L	0.519	1.00
247_V	353_L	0.518	1.00
120_L	124_A	0.516	1.00
306_K	326_S	0.516	1.00
241_S	245_I	0.515	1.00
99_Q	102_L	0.515	1.00
101_E	114_A	0.514	1.00
304_L	309_V	0.511	1.00
211_V	254_L	0.511	1.00
346_G	349_A	0.511	1.00
131_D	134_S	0.509	1.00
215_E	245_I	0.509	1.00
216_D	219_L	0.505	1.00
110_L	133_V	0.505	1.00
253_L	332_H	0.504	1.00
132_L	135_R	0.504	1.00
340_N	346_G	0.504	1.00
152_H	156_T	0.503	1.00
64_E	68_S	0.502	1.00
264_N	268_Y	0.501	1.00
284_G	355_K	0.501	1.00
71_V	75_F	0.500	1.00
186_M	190_V	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4i5sA	2	0.73	100	0.312	Contact Map
4bixA	2	0.6226	100	0.348	Contact Map
4ctiA	2	0.6253	100	0.367	Contact Map
4q20A	2	0.6226	100	0.413	Contact Map
3jz3A	2	0.4353	100	0.423	Contact Map
4mpcA	2	0.8154	100	0.433	Contact Map
2q8gA	2	0.8154	100	0.442	Contact Map
4gczA	2	0.8981	100	0.444	Contact Map
2c2aA	2	0.6253	100	0.448	Contact Map
2e0aA	2	0.8375	100	0.454	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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