GREMLIN Database
THIE - Thiamine-phosphate synthase
UniProt: P30137 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11586
Length: 211 (191)
Sequences: 5409 (4045)
Seq/√Len: 292.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
49_E77_K2.7781.00
136_T186_V2.6961.00
79_Q103_L2.4341.00
196_R200_A2.4091.00
52_V78_H2.2171.00
22_V54_A2.1921.00
45_D71_Y2.1901.00
44_R47_E2.1641.00
56_I60_R2.1461.00
29_L64_A2.1051.00
117_V121_A2.0811.00
172_A183_I2.0681.00
169_L201_Q2.0481.00
194_D197_L2.0321.00
99_R103_L1.9221.00
26_E30_D1.9011.00
87_Q114_E1.8681.00
132_F171_R1.8261.00
26_E61_R1.8251.00
99_R105_L1.8241.00
30_D196_R1.8151.00
173_P205_I1.8141.00
85_L95_L1.7751.00
153_V181_G1.7671.00
29_L58_L1.7661.00
119_L158_D1.7631.00
17_P190_T1.7231.00
17_P189_I1.6971.00
132_F174_A1.6801.00
131_V149_L1.6721.00
94_D97_A1.6651.00
60_R81_Y1.6501.00
153_V161_T1.6481.00
49_E53_V1.6431.00
59_G66_L1.5971.00
25_I55_A1.5861.00
130_H143_P1.5631.00
110_H114_E1.5471.00
129_G164_I1.5431.00
197_L201_Q1.5421.00
17_P33_V1.5361.00
29_L62_Y1.5151.00
112_D116_D1.4961.00
188_A202_L1.4751.00
106_G125_Y1.4441.00
31_A196_R1.4351.00
40_I43_R1.4191.00
119_L159_Y1.4101.00
116_D119_L1.3751.00
49_E78_H1.3721.00
168_S171_R1.3461.00
116_D155_R1.3461.00
30_D62_Y1.3441.00
31_A199_T1.3411.00
27_R196_R1.3131.00
56_I66_L1.3001.00
56_I59_G1.2901.00
48_V71_Y1.2881.00
86_G89_D1.2761.00
35_T67_F1.2731.00
91_Q94_D1.2721.00
192_A198_A1.2711.00
169_L202_L1.2561.00
107_V123_P1.2451.00
56_I81_Y1.2431.00
19_V36_L1.2431.00
92_A107_V1.2311.00
34_R203_L1.2221.00
72_W83_V1.2141.00
49_E74_L1.2051.00
83_V105_L1.2041.00
96_N100_A1.2001.00
111_D114_E1.1951.00
29_L59_G1.1911.00
33_V36_L1.1841.00
21_S40_I1.1651.00
198_A201_Q1.1641.00
50_A54_A1.1641.00
100_A103_L1.1621.00
38_L51_D1.1401.00
115_I152_H1.1341.00
26_E62_Y1.1341.00
151_R155_R1.1331.00
144_Q148_Q1.1291.00
174_A177_A1.1101.00
37_Q69_N1.1061.00
150_A154_E1.0941.00
47_E50_A1.0941.00
54_A57_A1.0931.00
38_L66_L1.0901.00
112_D151_R1.0881.00
26_E58_L1.0871.00
172_A205_I1.0851.00
112_D155_R1.0751.00
93_T121_A1.0641.00
133_P143_P1.0641.00
50_A53_V1.0631.00
135_Q187_S1.0601.00
38_L48_V1.0591.00
197_L200_A1.0581.00
147_E151_R1.0371.00
34_R64_A1.0361.00
116_D120_A1.0321.00
188_A198_A1.0311.00
22_V58_L1.0271.00
128_L161_T1.0261.00
162_V182_S1.0171.00
43_R47_E1.0091.00
145_G148_Q1.0041.00
56_I78_H0.9931.00
40_I51_D0.9901.00
71_Y74_L0.9861.00
189_I199_T0.9851.00
85_L107_V0.9801.00
21_S51_D0.9761.00
23_Q27_R0.9741.00
200_A204_E0.9701.00
43_R48_V0.9621.00
87_Q107_V0.9531.00
147_E150_A0.9501.00
150_A178_T0.9501.00
148_Q151_R0.9471.00
131_V163_A0.9431.00
45_D77_K0.9361.00
24_W190_T0.9361.00
112_D152_H0.9341.00
25_I36_L0.9311.00
58_L62_Y0.9301.00
201_Q205_I0.9261.00
38_L52_V0.9201.00
84_H125_Y0.9111.00
149_L163_A0.9101.00
95_L99_R0.9061.00
175_V180_V0.9041.00
76_I98_I0.8991.00
47_E51_D0.8961.00
109_T115_I0.8931.00
46_E50_A0.8891.00
117_V120_A0.8881.00
115_I156_L0.8871.00
52_V68_I0.8811.00
146_L178_T0.8811.00
113_M116_D0.8781.00
149_L153_V0.8771.00
19_V25_I0.8711.00
45_D73_R0.8701.00
16_Y37_Q0.8701.00
92_A105_L0.8631.00
172_A176_I0.8581.00
128_L152_H0.8551.00
57_A61_R0.8511.00
90_L98_I0.8491.00
57_A60_R0.8481.00
168_S187_S0.8481.00
199_T203_L0.8461.00
156_L159_Y0.8441.00
18_V186_V0.8431.00
168_S188_A0.8381.00
73_R77_K0.8341.00
18_V164_I0.8331.00
187_S191_Q0.8321.00
151_R154_E0.8261.00
74_L78_H0.8231.00
92_A123_P0.8131.00
28_L31_A0.8121.00
185_V189_I0.8101.00
67_F82_G0.8041.00
55_A66_L0.8010.99
68_I80_A0.7970.99
81_Y104_R0.7950.99
16_Y162_V0.7930.99
17_P28_L0.7930.99
131_V146_L0.7910.99
22_V51_D0.7760.99
67_F104_R0.7730.99
146_L175_V0.7700.99
33_V199_T0.7660.99
146_L174_A0.7660.99
70_D86_G0.7620.99
45_D90_L0.7610.99
29_L36_L0.7570.99
59_G62_Y0.7550.99
33_V189_I0.7540.99
167_I175_V0.7520.99
14_G203_L0.7490.99
21_S42_D0.7480.99
23_Q26_E0.7480.99
48_V69_N0.7460.99
128_L148_Q0.7450.99
173_P177_A0.7450.99
189_I198_A0.7440.99
48_V74_L0.7420.99
13_S182_S0.7380.99
115_I126_I0.7340.99
85_L105_L0.7320.99
185_V202_L0.7320.99
73_R76_I0.7210.99
18_V184_A0.7210.99
169_L198_A0.7180.99
161_T181_G0.7150.99
70_D89_D0.7110.99
201_Q204_E0.7060.99
91_Q117_V0.7030.99
113_M117_V0.6970.99
27_R31_A0.6950.99
171_R175_V0.6860.98
109_T118_A0.6850.98
92_A121_A0.6550.98
96_N99_R0.6530.98
83_V95_L0.6530.98
126_I161_T0.6470.98
195_W198_A0.6440.98
41_K137_K0.6430.98
96_N124_S0.6420.98
163_A180_V0.6390.98
153_V178_T0.6350.97
28_L189_I0.6340.97
27_R30_D0.6340.97
127_A164_I0.6310.97
53_V78_H0.6290.97
56_I80_A0.6260.97
126_I156_L0.6260.97
66_L81_Y0.6250.97
17_P36_L0.6220.97
52_V80_A0.6190.97
21_S43_R0.6140.97
20_D40_I0.6130.97
15_L203_L0.6110.97
167_I171_R0.6110.97
128_L144_Q0.6080.97
45_D48_V0.6010.96
104_R125_Y0.5880.96
45_D74_L0.5870.96
107_V121_A0.5870.96
53_V56_I0.5850.96
96_N105_L0.5850.96
149_L180_V0.5820.96
122_R159_Y0.5820.96
131_V145_G0.5790.96
16_Y35_T0.5790.96
34_R65_R0.5780.96
116_D156_L0.5780.96
109_T114_E0.5760.95
90_L94_D0.5750.95
26_E54_A0.5730.95
139_M142_A0.5720.95
38_L55_A0.5710.95
119_L156_L0.5670.95
135_Q138_Q0.5660.95
119_L122_R0.5640.95
37_Q127_A0.5620.95
32_G64_A0.5610.95
130_H140_P0.5600.95
36_L59_G0.5590.95
77_K94_D0.5580.95
169_L172_A0.5580.95
175_V183_I0.5530.94
27_R195_W0.5510.94
162_V181_G0.5490.94
41_K86_G0.5490.94
22_V55_A0.5470.94
146_L179_G0.5470.94
134_T187_S0.5430.94
40_I48_V0.5430.94
41_K70_D0.5370.93
34_R182_S0.5320.93
14_G34_R0.5300.93
72_W76_I0.5290.93
149_L178_T0.5290.93
74_L77_K0.5260.93
130_H142_A0.5250.93
38_L68_I0.5240.93
134_T145_G0.5240.93
67_F103_L0.5230.93
39_R137_K0.5230.93
110_H140_P0.5220.92
139_M143_P0.5210.92
198_A202_L0.5200.92
59_G64_A0.5200.92
176_I180_V0.5170.92
35_T65_R0.5140.92
126_I159_Y0.5130.92
13_S34_R0.5120.92
24_W195_W0.5110.92
118_A122_R0.5110.92
87_Q110_H0.5000.91
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nl6A 4 0.9668 100 0.313 Contact Map
1twdA 2 0.9242 100 0.412 Contact Map
3o63A 2 0.9336 100 0.422 Contact Map
3ceuA 1 0.8531 100 0.441 Contact Map
1yadA 2 0.8863 100 0.466 Contact Map
1xi3A 2 0.9336 100 0.468 Contact Map
2tpsA 1 0.9953 100 0.471 Contact Map
4wuiA 1 0.9052 100 0.475 Contact Map
2yw3A 4 0.9005 100 0.484 Contact Map
2bdqA 2 0.8389 100 0.49 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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