GREMLIN Database
LEU3 - 3-isopropylmalate dehydrogenase
UniProt: P30125 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG11577
Length: 363 (334)
Sequences: 6913 (3118)
Seq/√Len: 170.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
184_R218_D4.1841.00
177_R215_E3.8821.00
176_A209_V3.0641.00
22_Q352_G2.9781.00
177_R181_E2.8471.00
6_H39_T2.7521.00
25_K353_D2.7171.00
228_N232_Q2.6391.00
310_R323_E2.6031.00
332_E358_Y2.5781.00
328_R358_Y2.3971.00
28_D40_T2.3931.00
59_P63_E2.3671.00
169_R173_E2.3211.00
354_I357_R2.2991.00
20_M301_Q2.2621.00
22_Q25_K2.2261.00
9_V24_L2.2021.00
310_R320_C2.0981.00
191_T222_A2.0891.00
43_Y64_G2.0791.00
189_K222_A2.0531.00
209_V221_L2.0321.00
55_Q59_P2.0021.00
24_L42_H1.9881.00
135_L182_S1.9821.00
329_A358_Y1.9771.00
173_E211_E1.9701.00
8_A64_G1.9461.00
299_I355_I1.9431.00
315_A318_A1.9361.00
303_L326_I1.9351.00
44_D52_N1.9321.00
66_E102_F1.9161.00
176_A244_L1.9071.00
189_K220_E1.8951.00
334_I346_V1.8941.00
173_E208_I1.8851.00
302_I355_I1.8461.00
181_E216_Y1.8421.00
294_N349_D1.8371.00
236_D239_Q1.8111.00
294_N347_S1.8091.00
49_A58_P1.7881.00
17_P21_T1.7871.00
334_I354_I1.7581.00
15_I296_A1.7561.00
320_C324_R1.7501.00
210_N223_H1.7481.00
63_E67_Q1.7271.00
177_R216_Y1.7231.00
306_A323_E1.7181.00
211_E214_T1.7171.00
352_G356_A1.7171.00
169_R211_E1.7021.00
112_K327_N1.6761.00
173_E177_R1.6491.00
193_I229_A1.6381.00
66_E101_H1.6211.00
151_G207_E1.6041.00
208_I211_E1.5781.00
158_Y170_F1.5681.00
353_D357_R1.5631.00
316_D320_C1.5461.00
165_E246_S1.5401.00
108_L135_L1.5211.00
347_S350_E1.5191.00
115_Q331_E1.5091.00
8_A68_A1.5061.00
4_N37_R1.5031.00
41_S67_Q1.4801.00
5_Y315_A1.4801.00
62_V101_H1.4801.00
177_R212_I1.4631.00
63_E66_E1.4601.00
294_N348_T1.4591.00
4_N39_T1.4591.00
105_F274_E1.4561.00
30_V322_I1.4501.00
23_A305_L1.4441.00
234_I256_E1.4421.00
48_A52_N1.4401.00
212_I215_E1.4381.00
310_R319_A1.4371.00
36_M315_A1.4301.00
31_R38_I1.4241.00
97_P101_H1.4231.00
137_V178_I1.4231.00
192_S209_V1.4211.00
10_L45_V1.4111.00
181_E185_K1.3941.00
8_A43_Y1.3861.00
41_S68_A1.3821.00
40_T305_L1.3781.00
165_E201_S1.3771.00
29_A356_A1.3521.00
27_L38_I1.3491.00
25_K356_A1.3481.00
187_R241_D1.3471.00
24_L28_D1.3301.00
136_C254_S1.3301.00
36_M318_A1.3251.00
116_G331_E1.3231.00
356_A359_V1.3181.00
11_P42_H1.3091.00
23_A72_L1.2961.00
44_D53_H1.2911.00
295_I337_G1.2791.00
44_D49_A1.2781.00
299_I351_M1.2741.00
57_L62_V1.2641.00
329_A354_I1.2641.00
224_M232_Q1.2521.00
230_T253_L1.2291.00
41_S64_G1.2261.00
310_R316_D1.2261.00
137_V182_S1.2201.00
188_H241_D1.2171.00
176_A212_I1.2151.00
192_S223_H1.2071.00
6_H41_S1.2071.00
204_L208_I1.2061.00
159_E169_R1.2031.00
181_E184_R1.1991.00
24_L40_T1.1911.00
351_M355_I1.1841.00
308_L313_L1.1831.00
346_V354_I1.1831.00
180_F216_Y1.1761.00
324_R328_R1.1731.00
309_L318_A1.1671.00
65_C71_V1.1651.00
224_M228_N1.1651.00
23_A301_Q1.1641.00
5_Y313_L1.1561.00
26_V322_I1.1391.00
136_C257_C1.1291.00
224_M240_F1.1281.00
207_E211_E1.1231.00
224_M229_A1.1191.00
169_R208_I1.1181.00
49_A53_H1.1181.00
346_V350_E1.1181.00
53_H60_A1.0991.00
173_E212_I1.0981.00
115_Q130_N1.0951.00
134_I233_L1.0941.00
9_V42_H1.0941.00
163_D201_S1.0911.00
233_L243_L1.0861.00
328_R332_E1.0821.00
112_K133_D1.0781.00
172_I176_A1.0761.00
27_L305_L1.0761.00
350_E353_D1.0761.00
180_F219_V1.0751.00
186_R241_D1.0731.00
354_I358_Y1.0701.00
230_T256_E1.0671.00
8_A65_C1.0671.00
210_N213_A1.0641.00
108_L137_V1.0631.00
190_V221_L1.0611.00
290_I296_A1.0470.99
25_K349_D1.0470.99
103_K174_R1.0430.99
72_L305_L1.0420.99
321_A359_V1.0370.99
131_G238_S1.0300.99
196_A206_R1.0280.99
167_Y246_S1.0200.99
226_I252_I1.0190.99
144_I165_E1.0190.99
294_N350_E1.0120.99
21_T25_K0.9910.99
149_P164_T0.9850.99
144_I246_S0.9810.99
31_R36_M0.9810.99
140_L271_S0.9780.99
30_V318_A0.9770.99
160_K166_V0.9730.99
23_A302_I0.9710.99
51_D80_K0.9690.99
22_Q349_D0.9680.99
232_Q239_Q0.9670.99
73_F95_L0.9640.99
60_A63_E0.9620.99
184_R188_H0.9570.99
58_P61_T0.9540.99
299_I330_L0.9520.99
193_I226_I0.9520.99
222_A239_Q0.9520.99
25_K352_G0.9520.99
182_S186_R0.9500.99
26_V356_A0.9450.99
180_F213_A0.9430.99
106_S175_I0.9430.99
184_R219_V0.9420.99
318_A321_A0.9410.99
190_V212_I0.9400.99
49_A61_T0.9360.99
26_V359_V0.9350.99
143_G165_E0.9310.99
303_L323_E0.9300.99
170_F173_E0.9300.99
290_I295_I0.9240.99
184_R216_Y0.9160.99
134_I257_C0.9130.99
31_R37_R0.9120.99
179_A190_V0.9110.99
22_Q294_N0.9110.99
194_D206_R0.9100.98
235_K259_M0.9090.98
340_A346_V0.9070.98
55_Q88_Q0.9050.98
167_Y172_I0.9050.98
209_V244_L0.9040.98
273_N277_F0.9020.98
9_V20_M0.9000.98
6_H67_Q0.8990.98
11_P21_T0.8980.98
112_K323_E0.8960.98
232_Q236_D0.8960.98
180_F190_V0.8940.98
299_I326_I0.8910.98
55_Q97_P0.8900.98
330_L335_R0.8900.98
190_V242_V0.8890.98
161_A204_L0.8830.98
45_V98_L0.8810.98
320_C323_E0.8780.98
19_V301_Q0.8730.98
26_V302_I0.8720.98
306_A310_R0.8710.98
290_I337_G0.8700.98
165_E202_S0.8670.98
43_Y68_A0.8650.98
190_V244_L0.8580.98
143_G246_S0.8560.98
51_D78_G0.8540.98
282_P301_Q0.8540.98
328_R331_E0.8530.98
60_A64_G0.8530.98
30_V36_M0.8490.98
108_L178_I0.8470.98
43_Y61_T0.8460.98
302_I326_I0.8380.97
329_A355_I0.8340.97
9_V40_T0.8320.97
64_G68_A0.8300.97
22_Q353_D0.8290.97
111_A257_C0.8280.97
19_V74_G0.8270.97
106_S178_I0.8240.97
192_S221_L0.8240.97
114_Y327_N0.8150.97
202_S249_F0.8120.97
325_A329_A0.8110.97
115_Q327_N0.8090.97
88_Q92_R0.8090.97
45_V73_F0.8060.97
75_S284_G0.8040.97
116_G327_N0.8020.97
196_A225_Y0.7980.97
289_D337_G0.7970.97
103_K141_T0.7960.96
23_A27_L0.7950.96
160_K168_H0.7940.96
270_A280_Y0.7910.96
194_D223_H0.7880.96
305_L308_L0.7870.96
10_L65_C0.7850.96
30_V305_L0.7850.96
193_I243_L0.7840.96
20_M74_G0.7830.96
50_I56_P0.7830.96
93_G97_P0.7810.96
150_K162_F0.7780.96
226_I230_T0.7770.96
193_I253_L0.7760.96
324_R327_N0.7740.96
185_K314_D0.7730.96
190_V219_V0.7720.96
176_A221_L0.7700.96
22_Q356_A0.7690.96
329_A334_I0.7650.96
191_T224_M0.7590.95
47_G78_G0.7590.95
144_I248_L0.7560.95
62_V66_E0.7540.95
336_T351_M0.7530.95
47_G51_D0.7510.95
346_V351_M0.7470.95
18_E292_G0.7460.95
208_I212_I0.7460.95
70_A313_L0.7430.95
5_Y36_M0.7430.95
38_I305_L0.7420.95
229_A243_L0.7410.95
165_E248_L0.7370.95
179_A244_L0.7360.95
111_A261_T0.7360.95
151_G161_A0.7330.94
161_A201_S0.7330.94
44_D48_A0.7330.94
28_D31_R0.7320.94
210_N221_L0.7320.94
21_T349_D0.7290.94
27_L309_L0.7280.94
337_G347_S0.7260.94
233_L257_C0.7260.94
113_L132_F0.7240.94
136_C253_L0.7210.94
86_P92_R0.7210.94
153_E160_K0.7200.94
133_D241_D0.7200.94
349_D353_D0.7190.94
226_I246_S0.7190.94
353_D356_A0.7180.94
212_I216_Y0.7180.94
90_P93_G0.7180.94
107_N283_A0.7180.94
169_R204_L0.7170.94
142_G171_E0.7170.94
111_A134_I0.7150.94
87_D92_R0.7150.94
134_I260_I0.7150.94
268_P307_L0.7120.94
268_P303_L0.7120.94
21_T42_H0.7110.94
293_K337_G0.7090.93
72_L301_Q0.7080.93
104_L279_L0.7070.93
43_Y60_A0.7020.93
66_E277_F0.7000.93
129_A238_S0.6980.93
137_V175_I0.6970.93
64_G67_Q0.6970.93
287_A290_I0.6950.93
55_Q60_A0.6920.93
305_L309_L0.6900.93
45_V57_L0.6900.93
178_I182_S0.6890.92
77_G286_S0.6890.92
180_F221_L0.6790.92
173_E214_T0.6780.92
317_D320_C0.6770.92
289_D296_A0.6760.92
341_R346_V0.6750.92
181_E215_E0.6750.92
349_D352_G0.6700.91
20_M23_A0.6680.91
228_N264_M0.6680.91
165_E249_F0.6680.91
280_Y311_Y0.6670.91
75_S301_Q0.6660.91
229_A240_F0.6660.91
319_A323_E0.6650.91
71_V279_L0.6640.91
47_G77_G0.6630.91
175_I244_L0.6600.91
45_V93_G0.6550.90
22_Q302_I0.6540.90
118_E130_N0.6520.90
178_I274_E0.6510.90
194_D225_Y0.6500.90
329_A351_M0.6490.90
172_I208_I0.6490.90
78_G93_G0.6490.90
313_L316_D0.6480.90
27_L40_T0.6480.90
56_P88_Q0.6460.90
302_I322_I0.6460.90
270_A307_L0.6430.90
113_L118_E0.6430.90
48_A51_D0.6430.90
163_D203_I0.6420.90
206_R210_N0.6400.89
232_Q235_K0.6400.89
159_E162_F0.6360.89
155_S160_K0.6350.89
337_G342_G0.6330.89
92_R97_P0.6330.89
213_A221_L0.6330.89
122_P129_A0.6320.89
283_A300_A0.6310.89
7_I38_I0.6310.89
30_V321_A0.6300.89
155_S158_Y0.6300.89
263_S300_A0.6290.89
57_L98_L0.6260.88
327_N331_E0.6240.88
89_Q93_G0.6230.88
89_Q92_R0.6230.88
266_M339_L0.6210.88
114_Y303_L0.6200.88
277_F280_Y0.6190.88
335_R339_L0.6170.88
25_K28_D0.6150.87
74_G301_Q0.6140.87
151_G154_G0.6130.87
172_I246_S0.6130.87
195_K227_D0.6110.87
59_P97_P0.6110.87
136_C243_L0.6100.87
342_G346_V0.6090.87
20_M24_L0.6080.87
192_S205_W0.6070.87
44_D60_A0.6020.86
27_L322_I0.6020.86
272_L311_Y0.6020.86
193_I245_C0.6000.86
103_K273_N0.5950.86
149_P162_F0.5950.86
234_I260_I0.5930.86
135_L242_V0.5900.85
10_L43_Y0.5900.85
213_A217_P0.5880.85
103_K170_F0.5860.85
133_D187_R0.5840.85
296_A337_G0.5830.85
149_P152_R0.5810.84
148_Q166_V0.5800.84
50_I88_Q0.5800.84
135_L186_R0.5780.84
7_I308_L0.5780.84
151_G200_Q0.5760.84
336_T339_L0.5750.84
116_G119_A0.5730.84
309_L322_I0.5720.83
268_P300_A0.5720.83
183_A190_V0.5700.83
321_A325_A0.5690.83
171_E174_R0.5670.83
334_I351_M0.5670.83
178_I181_E0.5670.83
317_D321_A0.5660.83
202_S246_S0.5650.83
65_C68_A0.5640.83
91_E94_A0.5620.82
49_A57_L0.5610.82
42_H48_A0.5600.82
229_A253_L0.5590.82
188_H218_D0.5560.82
309_L319_A0.5550.82
228_N239_Q0.5540.82
7_I40_T0.5540.82
38_I318_A0.5520.81
61_T94_A0.5500.81
49_A52_N0.5490.81
145_Y281_E0.5480.81
17_P48_A0.5480.81
103_K142_G0.5470.81
132_F238_S0.5460.81
302_I352_G0.5440.80
158_Y168_H0.5430.80
20_M72_L0.5430.80
105_F175_I0.5380.80
159_E207_E0.5380.80
43_Y58_P0.5380.80
86_P203_I0.5380.80
65_C98_L0.5370.79
152_R207_E0.5370.79
21_T24_L0.5370.79
135_L187_R0.5350.79
246_S249_F0.5340.79
272_L278_G0.5340.79
278_G311_Y0.5340.79
228_N240_F0.5300.79
335_R346_V0.5290.78
38_I315_A0.5280.78
8_A41_S0.5250.78
148_Q164_T0.5230.78
334_I358_Y0.5230.78
118_E129_A0.5230.78
334_I357_R0.5220.78
73_F98_L0.5220.78
184_R190_V0.5180.77
18_E294_N0.5180.77
118_E339_L0.5170.77
323_E327_N0.5150.77
105_F178_I0.5140.76
184_R217_P0.5130.76
263_S266_M0.5130.76
163_D199_L0.5120.76
335_R351_M0.5120.76
23_A26_V0.5120.76
57_L61_T0.5120.76
251_D255_D0.5120.76
113_L131_G0.5110.76
65_C102_F0.5110.76
176_A208_I0.5110.76
57_L97_P0.5100.76
273_N278_G0.5100.76
45_V61_T0.5090.76
79_P283_A0.5070.76
30_V302_I0.5050.75
137_V179_A0.5040.75
210_N214_T0.5030.75
76_V286_S0.5010.75
62_V97_P0.5000.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1cnzA 2 0.9945 100 0.029 Contact Map
3uduA 3 0.978 100 0.032 Contact Map
3vmkA 2 0.9945 100 0.036 Contact Map
1a05A 2 0.978 100 0.038 Contact Map
2y3zA 2 0.9614 100 0.04 Contact Map
3u1hA 2 0.9614 100 0.041 Contact Map
3r8wA 2 0.978 100 0.046 Contact Map
1vlcA 2 0.9835 100 0.05 Contact Map
4xxvA 2 0.9669 100 0.057 Contact Map
3flkA 3 0.9614 100 0.058 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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